AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0737730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0737730 MtrunA17_Chr8g0342381 87.245 196 24 1 1 195 1 196 7.34e-73 218
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0737730 AT1G55080.1 65.979 97 33 0 98 194 147 243 1.91e-40 137
Msa0737730 AT1G55080.2 63.934 61 22 0 98 158 147 207 4.40e-21 87.4
Msa0737730 AT1G29580.1 42.857 98 27 2 98 195 21 89 2.45e-12 60.8

Find 69 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTTGGGGATTGGAATT+AGG 0.122258 5_1:-55173682 None:intergenic
GAATTTGGGGATTGGAATTA+GGG 0.195514 5_1:-55173681 None:intergenic
ATTGAGATTGAGATTGAATT+TGG 0.237671 5_1:-55173696 None:intergenic
ATTATGGAAAGAACGGAATT+TGG 0.239104 5_1:+55177129 Msa0737730:three_prime_UTR
CTGTAGAAGTTGGGCAATTA+TGG 0.241267 5_1:+55177113 Msa0737730:three_prime_UTR
TTGAGATTGAGATTGAATTT+GGG 0.281993 5_1:-55173695 None:intergenic
GGTAATTTGAAGGGTAATAA+TGG 0.289807 5_1:+55177150 Msa0737730:three_prime_UTR
TTGTGATTTGGGATCGATTT+GGG 0.306504 5_1:-55172596 None:intergenic
GTTCGTTGAATTTGTGATTT+GGG 0.310001 5_1:-55172607 None:intergenic
AGTTCGTTGAATTTGTGATT+TGG 0.327762 5_1:-55172608 None:intergenic
CGAAAATGGATTATACGAAT+TGG 0.328441 5_1:-55172637 None:intergenic
AAAAGAATGACAGGCTCTTA+TGG 0.329781 5_1:-55176270 None:intergenic
TGACGAGAGGAGTACGAAAA+TGG 0.332284 5_1:-55172651 None:intergenic
GCTGCTGACACTTCTCAAAA+TGG 0.340809 5_1:-55174343 None:intergenic
GTTACCTAAAATAAAGTTAA+GGG 0.352129 5_1:-55177324 None:intergenic
GAAAATGGATTATACGAATT+GGG 0.354385 5_1:-55172636 None:intergenic
AATGCATCAGATTGCTGATC+TGG 0.355987 5_1:-55174098 None:intergenic
ACTTTAAACTTCCCAGTTCT+CGG 0.367408 5_1:+55176954 Msa0737730:three_prime_UTR
AGTTACCTAAAATAAAGTTA+AGG 0.385388 5_1:-55177325 None:intergenic
CCAGTTCTCGGAGAATCGCT+TGG 0.393069 5_1:+55176966 Msa0737730:three_prime_UTR
CCAAGCGATTCTCCGAGAAC+TGG 0.396456 5_1:-55176966 None:intergenic
GATAAAGTTGTTGTTGTTGT+TGG 0.407429 5_1:-55173825 None:intergenic
AACGGAATTTGGTAATTTGA+AGG 0.417164 5_1:+55177140 Msa0737730:three_prime_UTR
CAGTTCTCGGAGAATCGCTT+GGG 0.432041 5_1:+55176967 Msa0737730:three_prime_UTR
TTGAGATTGAATTTGGGGAT+TGG 0.432517 5_1:-55173689 None:intergenic
GGGGATTGGAATTAGGGGTT+GGG 0.433474 5_1:-55173675 None:intergenic
TTTGTGATTTGGGATCGATT+TGG 0.440842 5_1:-55172597 None:intergenic
GTTGTTTGAGGTTGTTGAAA+AGG 0.447188 5_1:-55173790 None:intergenic
TGCTTAATCCAGACTGTTGA+GGG 0.453838 5_1:+55176068 Msa0737730:intron
TTGTGCTTTAATTAATCCAT+TGG 0.455549 5_1:+55177191 Msa0737730:three_prime_UTR
TGGGGATTGGAATTAGGGGT+TGG 0.459404 5_1:-55173676 None:intergenic
AATTGAGATTGTGTTGTTTG+AGG 0.469049 5_1:-55173802 None:intergenic
CTACTTGAGTCTGTAGAAGT+TGG 0.475403 5_1:+55177103 Msa0737730:three_prime_UTR
TACTTGAGTCTGTAGAAGTT+GGG 0.476025 5_1:+55177104 Msa0737730:three_prime_UTR
TGGATTAATTAAAGCACAAA+GGG 0.481696 5_1:-55177187 None:intergenic
ACCTGTCTGAAAAGAATGAC+AGG 0.497342 5_1:-55176279 None:intergenic
AGATTCTGCTGTTGCTGTGG+AGG 0.503588 5_1:-55173901 None:intergenic
ACGGAATTTGGTAATTTGAA+GGG 0.505089 5_1:+55177141 Msa0737730:three_prime_UTR
TTCTATTAGCACCAAGGCTA+TGG 0.509576 5_1:+55174385 Msa0737730:CDS
ATGCATCAGATTGCTGATCT+GGG 0.510707 5_1:-55174097 None:intergenic
TTTCGTACTCCTCTCGTCAC+AGG 0.515867 5_1:+55172655 Msa0737730:five_prime_UTR
ATGGAGCATTACTCTTCTCC+TGG 0.537394 5_1:+55173634 Msa0737730:CDS
GTTTCCATGTTCAACAACTT+CGG 0.540555 5_1:-55174075 None:intergenic
CAAGCGATTCTCCGAGAACT+GGG 0.542801 5_1:-55176965 None:intergenic
AAGGCTCTTAATTAGACCAA+TGG 0.543615 5_1:-55177207 None:intergenic
AGATCAGCAATCTGATGCAT+TGG 0.543969 5_1:+55174100 Msa0737730:CDS
TTAGCCGAAGTTGTTGAACA+TGG 0.546188 5_1:+55174071 Msa0737730:CDS
TGAAGATTCTGCTGTTGCTG+TGG 0.547880 5_1:-55173904 None:intergenic
AGCAATTGCTAAATCAAAGA+AGG 0.550496 5_1:+55176117 Msa0737730:CDS
AGAGATAGATACCATAGCCT+TGG 0.550759 5_1:-55174396 None:intergenic
TGAGATTGAGATTGAATTTG+GGG 0.552572 5_1:-55173694 None:intergenic
TTGCTTAATCCAGACTGTTG+AGG 0.552674 5_1:+55176067 Msa0737730:intron
ACTCTTCTCCTGGTGGAACA+TGG 0.560525 5_1:+55173644 Msa0737730:CDS
GGGATCGATTTGGGCTTCAG+AGG 0.560901 5_1:-55172587 None:intergenic
TTGGGCAATTATGGAAAGAA+CGG 0.563893 5_1:+55177122 Msa0737730:three_prime_UTR
AAAATGGATTATACGAATTG+GGG 0.567140 5_1:-55172635 None:intergenic
GTAGAGCCAACAGAAGAAAG+TGG 0.570239 5_1:-55177084 None:intergenic
CACAGTTGTTAAAATTCCGT+AGG 0.571627 5_1:+55177013 Msa0737730:three_prime_UTR
TTACCTAAAATAAAGTTAAG+GGG 0.574712 5_1:-55177323 None:intergenic
ATGGATTAATTAAAGCACAA+AGG 0.586226 5_1:-55177188 None:intergenic
ATCATCGTCCATGTTCCACC+AGG 0.591081 5_1:-55173652 None:intergenic
AGTAACTTGCAGAATACCTA+CGG 0.604437 5_1:-55177029 None:intergenic
CAATTACACAAAATCTGTAG+AGG 0.606651 5_1:+55176230 Msa0737730:CDS
CTCTGCTTCTATTAGCACCA+AGG 0.627673 5_1:+55174379 Msa0737730:CDS
GAGGGATCGAATTGAAGCGA+AGG 0.634046 5_1:+55173596 Msa0737730:five_prime_UTR
AATTTGGGGATTGGAATTAG+GGG 0.642153 5_1:-55173680 None:intergenic
GAGCATTACTCTTCTCCTGG+TGG 0.662821 5_1:+55173637 Msa0737730:CDS
ATGAGCATACCTGTGACGAG+AGG 0.671781 5_1:-55172664 None:intergenic
AAGGTGTAAAGATAGAACAA+TGG 0.674984 5_1:+55173615 Msa0737730:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAATAGTTAAAAGA+GGG + chr5_1:55173578-55173597 Msa0737730:intron 10.0%
!! AATAATAGAAATGAAAATTA+GGG - chr5_1:55173989-55174008 None:intergenic 10.0%
!! AAATAATAGAAATGAAAATT+AGG - chr5_1:55173990-55174009 None:intergenic 10.0%
!!! TTTAATCAATTTTTTTTTAC+CGG + chr5_1:55174181-55174200 Msa0737730:intron 10.0%
!! AAGTAATTTGATATTATATA+GGG - chr5_1:55176834-55176853 None:intergenic 10.0%
!! AAAGTAATTTGATATTATAT+AGG - chr5_1:55176835-55176854 None:intergenic 10.0%
!!! AGTATTTATTTTTATGAGAA+TGG + chr5_1:55173248-55173267 Msa0737730:intron 15.0%
!!! GTATTTATTTTTATGAGAAT+GGG + chr5_1:55173249-55173268 Msa0737730:intron 15.0%
!!! TATATTATTTTTGATAGACT+AGG - chr5_1:55173301-55173320 None:intergenic 15.0%
!! AAAATAATATAAAGCAAACA+CGG + chr5_1:55173335-55173354 Msa0737730:intron 15.0%
!! AAAAACATGAATATCATTAA+AGG - chr5_1:55173401-55173420 None:intergenic 15.0%
!!! TAATTTCTTTTTGTTTTTGT+TGG + chr5_1:55173555-55173574 Msa0737730:intron 15.0%
!! GTTAAAAAAATAGTTAAAAG+AGG + chr5_1:55173577-55173596 Msa0737730:intron 15.0%
!!! TTATTTTTGTTTGATTTTGT+TGG + chr5_1:55174297-55174316 Msa0737730:intron 15.0%
!! TTAACCAATAACAAATTTAA+AGG - chr5_1:55174617-55174636 None:intergenic 15.0%
!!! TGTGTTTTTAATATTATTTC+AGG + chr5_1:55175740-55175759 Msa0737730:intron 15.0%
!! AACAAATAAAATCTCATTTA+AGG - chr5_1:55176311-55176330 None:intergenic 15.0%
!!! TTTTATTTGTTAATAGATTG+CGG + chr5_1:55176320-55176339 Msa0737730:intron 15.0%
!!! TTCATTTCATCTAAATTTTA+AGG - chr5_1:55176383-55176402 None:intergenic 15.0%
!! TGGTATGAAATTTAATAAAA+AGG - chr5_1:55176754-55176773 None:intergenic 15.0%
!! AAATAAAAAACTACAACAAA+AGG - chr5_1:55177229-55177248 None:intergenic 15.0%
!! AATGGAAATAATACAACAAA+GGG - chr5_1:55172838-55172857 None:intergenic 20.0%
!! AAATGGAAATAATACAACAA+AGG - chr5_1:55172839-55172858 None:intergenic 20.0%
!!! CTTTTTATTAATTCATCTCA+TGG + chr5_1:55174239-55174258 Msa0737730:intron 20.0%
!! AAAATAGTCAAAAGTTGTAT+TGG + chr5_1:55174489-55174508 Msa0737730:intron 20.0%
!!! TTGACCTTTAAATTTGTTAT+TGG + chr5_1:55174610-55174629 Msa0737730:intron 20.0%
!!! TTTTTTAAAACCATAATCGA+TGG - chr5_1:55174773-55174792 None:intergenic 20.0%
!! ATTAGATTAAAAGAAAACAG+TGG + chr5_1:55175603-55175622 Msa0737730:intron 20.0%
!! TTAGATTAAAAGAAAACAGT+GGG + chr5_1:55175604-55175623 Msa0737730:intron 20.0%
!!! TGAATTGTATATTTGCATTT+TGG + chr5_1:55176631-55176650 Msa0737730:intron 20.0%
!! ATTTAATAAAAAGGACCATT+AGG - chr5_1:55176745-55176764 None:intergenic 20.0%
!! TTACCTAAAATAAAGTTAAG+GGG - chr5_1:55177326-55177345 None:intergenic 20.0%
!! GTTACCTAAAATAAAGTTAA+GGG - chr5_1:55177327-55177346 None:intergenic 20.0%
!! AGTTACCTAAAATAAAGTTA+AGG - chr5_1:55177328-55177347 None:intergenic 20.0%
!!! AAAATGGATTATACGAATTG+GGG - chr5_1:55172638-55172657 None:intergenic 25.0%
!!! GAAAATGGATTATACGAATT+GGG - chr5_1:55172639-55172658 None:intergenic 25.0%
! TGTTGTATTATTTCCATTTC+TGG + chr5_1:55172840-55172859 Msa0737730:intron 25.0%
! GTTGTATTATTTCCATTTCT+GGG + chr5_1:55172841-55172860 Msa0737730:intron 25.0%
!!! TGTATTGTTTTGTATCAATC+TGG + chr5_1:55173116-55173135 Msa0737730:intron 25.0%
!! GGTTAACTTATACTCTTTTT+AGG + chr5_1:55173156-55173175 Msa0737730:intron 25.0%
!!! ACTCTTTTTAGGTATAAAGT+TGG + chr5_1:55173167-55173186 Msa0737730:intron 25.0%
! TTGAGATTGAGATTGAATTT+GGG - chr5_1:55173698-55173717 None:intergenic 25.0%
! ATTGAGATTGAGATTGAATT+TGG - chr5_1:55173699-55173718 None:intergenic 25.0%
!! TGAAAATTAGGGTTTTTGAA+GGG - chr5_1:55173978-55173997 None:intergenic 25.0%
!! ATGAAAATTAGGGTTTTTGA+AGG - chr5_1:55173979-55173998 None:intergenic 25.0%
! TAATTGAAGATTTCTTCTTG+TGG + chr5_1:55174030-55174049 Msa0737730:intron 25.0%
! TACCATTAAATTACCATCAA+AGG + chr5_1:55174724-55174743 Msa0737730:intron 25.0%
! TTGATCTTACCACAAAAAAT+TGG + chr5_1:55175250-55175269 Msa0737730:intron 25.0%
! TGATCTTACCACAAAAAATT+GGG + chr5_1:55175251-55175270 Msa0737730:intron 25.0%
!! TTTTCACATCATTATCTCAA+TGG + chr5_1:55175342-55175361 Msa0737730:intron 25.0%
! ATACATAGACTCAAGAAATA+AGG + chr5_1:55175440-55175459 Msa0737730:intron 25.0%
! ATTCTTTAACAAATGCCTTA+GGG - chr5_1:55175784-55175803 None:intergenic 25.0%
! ATTATTGTTTATCATGCACA+GGG - chr5_1:55175920-55175939 None:intergenic 25.0%
! AATTATTGTTTATCATGCAC+AGG - chr5_1:55175921-55175940 None:intergenic 25.0%
!!! AACTCTTTGAAGTATTAATC+AGG - chr5_1:55176441-55176460 None:intergenic 25.0%
!!! TTGTATATTTGCATTTTGGT+TGG + chr5_1:55176635-55176654 Msa0737730:intron 25.0%
! ATTAGGAAACCATCTTATAT+GGG - chr5_1:55176728-55176747 None:intergenic 25.0%
! TGGATTAATTAAAGCACAAA+GGG - chr5_1:55177190-55177209 None:intergenic 25.0%
!! ATGGATTAATTAAAGCACAA+AGG - chr5_1:55177191-55177210 None:intergenic 25.0%
! TTGTGCTTTAATTAATCCAT+TGG + chr5_1:55177191-55177210 Msa0737730:three_prime_UTR 25.0%
!! GTTTTGTAACAGAAAAACTT+TGG + chr5_1:55177283-55177302 Msa0737730:three_prime_UTR 25.0%
GTTCGTTGAATTTGTGATTT+GGG - chr5_1:55172610-55172629 None:intergenic 30.0%
AGTTCGTTGAATTTGTGATT+TGG - chr5_1:55172611-55172630 None:intergenic 30.0%
!! CGAAAATGGATTATACGAAT+TGG - chr5_1:55172640-55172659 None:intergenic 30.0%
! CTTATAGCTTTTCGATTTCT+GGG + chr5_1:55172747-55172766 Msa0737730:intron 30.0%
TTTCGATTTCAAATCCACAA+AGG + chr5_1:55172788-55172807 Msa0737730:intron 30.0%
! TTTTGTATGAAACCCAGAAA+TGG - chr5_1:55172856-55172875 None:intergenic 30.0%
TTTCTGGGTTTCATACAAAA+AGG + chr5_1:55172856-55172875 Msa0737730:intron 30.0%
!!! TTTCTTGTTTTGCTTGTTTC+TGG + chr5_1:55172941-55172960 Msa0737730:intron 30.0%
!! CTGGTAATGTCATGTATTTT+AGG + chr5_1:55173135-55173154 Msa0737730:intron 30.0%
ACTAAGTAGGCTTATATATG+AGG - chr5_1:55173425-55173444 None:intergenic 30.0%
AAAGTTTGAGTATCATTAGC+TGG + chr5_1:55173485-55173504 Msa0737730:intron 30.0%
AAGGTGTAAAGATAGAACAA+TGG + chr5_1:55173615-55173634 Msa0737730:exon 30.0%
TGAGATTGAGATTGAATTTG+GGG - chr5_1:55173697-55173716 None:intergenic 30.0%
!! AATTGAGATTGTGTTGTTTG+AGG - chr5_1:55173805-55173824 None:intergenic 30.0%
! GATAAAGTTGTTGTTGTTGT+TGG - chr5_1:55173828-55173847 None:intergenic 30.0%
!! GAAAATTAGGGTTTTTGAAG+GGG - chr5_1:55173977-55173996 None:intergenic 30.0%
TTTCTTCTTGTGGATATAGT+TGG + chr5_1:55174040-55174059 Msa0737730:intron 30.0%
! TCATACTACATTGATGATCA+AGG - chr5_1:55174470-55174489 None:intergenic 30.0%
AATCGAAGTATATGAAGAGA+AGG + chr5_1:55174543-55174562 Msa0737730:intron 30.0%
ATCGAAGTATATGAAGAGAA+GGG + chr5_1:55174544-55174563 Msa0737730:intron 30.0%
!! ATACTTACTTCTAGAGTCAA+CGG - chr5_1:55174572-55174591 None:intergenic 30.0%
! TGATGGTGAAAAAGTCAAAT+TGG + chr5_1:55174815-55174834 Msa0737730:intron 30.0%
GATGGTGAAAAAGTCAAATT+GGG + chr5_1:55174816-55174835 Msa0737730:intron 30.0%
CACTGAATTTCGTTATTCTA+CGG + chr5_1:55175182-55175201 Msa0737730:intron 30.0%
ATGATGTGAAAACAACTACT+AGG - chr5_1:55175334-55175353 None:intergenic 30.0%
CATACCTTGACAATTAGAAA+AGG + chr5_1:55175386-55175405 Msa0737730:intron 30.0%
! CATTCCTTTTCTAATTGTCA+AGG - chr5_1:55175393-55175412 None:intergenic 30.0%
TGTAAAGTAACAGAAGAACT+AGG + chr5_1:55175502-55175521 Msa0737730:intron 30.0%
ACAAAATCACACACATATCT+AGG - chr5_1:55175663-55175682 None:intergenic 30.0%
! TGTGTGATTTTGTGTCTAAA+TGG + chr5_1:55175670-55175689 Msa0737730:intron 30.0%
! CTGTTAATACTGTTTAGTTC+TGG + chr5_1:55175698-55175717 Msa0737730:intron 30.0%
GATTCTTTAACAAATGCCTT+AGG - chr5_1:55175785-55175804 None:intergenic 30.0%
GGGCATGTTATGAAATATAA+AGG - chr5_1:55175900-55175919 None:intergenic 30.0%
!! CTTAACGTTTTAATGGTTTG+AGG - chr5_1:55175993-55176012 None:intergenic 30.0%
! CGAAAGACTTAACGTTTTAA+TGG - chr5_1:55176000-55176019 None:intergenic 30.0%
CAACCAAAAAGCTATAAGTT+AGG - chr5_1:55176050-55176069 None:intergenic 30.0%
AGCAATTGCTAAATCAAAGA+AGG + chr5_1:55176117-55176136 Msa0737730:CDS 30.0%
TTGTATTAGACATCTCTGTT+TGG + chr5_1:55176150-55176169 Msa0737730:intron 30.0%
! CTCTACAGATTTTGTGTAAT+TGG - chr5_1:55176232-55176251 None:intergenic 30.0%
CAATTACACAAAATCTGTAG+AGG + chr5_1:55176230-55176249 Msa0737730:CDS 30.0%
AATCCACATCAAGTAAAACA+TGG - chr5_1:55176349-55176368 None:intergenic 30.0%
CATTATCTGCGTATGTTAAA+GGG + chr5_1:55176479-55176498 Msa0737730:intron 30.0%
TTAGGAAACCATCTTATATG+GGG - chr5_1:55176727-55176746 None:intergenic 30.0%
CATTAGGAAACCATCTTATA+TGG - chr5_1:55176729-55176748 None:intergenic 30.0%
CATATAAGATGGTTTCCTAA+TGG + chr5_1:55176727-55176746 Msa0737730:intron 30.0%
GTCAAAACTAAGTCAGAAAA+AGG - chr5_1:55176902-55176921 None:intergenic 30.0%
!! GGAATTTTAACAACTGTGAA+AGG - chr5_1:55177011-55177030 None:intergenic 30.0%
ATTATGGAAAGAACGGAATT+TGG + chr5_1:55177129-55177148 Msa0737730:three_prime_UTR 30.0%
! AACGGAATTTGGTAATTTGA+AGG + chr5_1:55177140-55177159 Msa0737730:three_prime_UTR 30.0%
! ACGGAATTTGGTAATTTGAA+GGG + chr5_1:55177141-55177160 Msa0737730:three_prime_UTR 30.0%
GGTAATTTGAAGGGTAATAA+TGG + chr5_1:55177150-55177169 Msa0737730:three_prime_UTR 30.0%
GGGTAATAATGGTGTAAAAA+TGG + chr5_1:55177161-55177180 Msa0737730:three_prime_UTR 30.0%
!!! TAGCCCCTTAACTTTATTTT+AGG + chr5_1:55177320-55177339 Msa0737730:three_prime_UTR 30.0%
! TTGTGATTTGGGATCGATTT+GGG - chr5_1:55172599-55172618 None:intergenic 35.0%
! TTTGTGATTTGGGATCGATT+TGG - chr5_1:55172600-55172619 None:intergenic 35.0%
! GCTTATAGCTTTTCGATTTC+TGG + chr5_1:55172746-55172765 Msa0737730:intron 35.0%
ATAGAATATGCTCACATAGC+AGG + chr5_1:55173044-55173063 Msa0737730:intron 35.0%
GTCCTTAAGATTAGCAATGT+TGG + chr5_1:55173456-55173475 Msa0737730:intron 35.0%
AGCCAACATTGCTAATCTTA+AGG - chr5_1:55173461-55173480 None:intergenic 35.0%
AATTTGGGGATTGGAATTAG+GGG - chr5_1:55173683-55173702 None:intergenic 35.0%
GAATTTGGGGATTGGAATTA+GGG - chr5_1:55173684-55173703 None:intergenic 35.0%
TGAATTTGGGGATTGGAATT+AGG - chr5_1:55173685-55173704 None:intergenic 35.0%
TTGAGATTGAATTTGGGGAT+TGG - chr5_1:55173692-55173711 None:intergenic 35.0%
! GTTGTTTGAGGTTGTTGAAA+AGG - chr5_1:55173793-55173812 None:intergenic 35.0%
GTTTCCATGTTCAACAACTT+CGG - chr5_1:55174078-55174097 None:intergenic 35.0%
TTCAACGTAATCACATGTGT+CGG - chr5_1:55174153-55174172 None:intergenic 35.0%
TAAAATCACAGCGCAATGAA+AGG - chr5_1:55174431-55174450 None:intergenic 35.0%
! GGCCTTTGATGGTAATTTAA+TGG - chr5_1:55174729-55174748 None:intergenic 35.0%
TTGTCAAATAGCTGCTATTG+CGG - chr5_1:55174929-55174948 None:intergenic 35.0%
ACGAAATTCAGTGTTGTCTA+TGG - chr5_1:55175175-55175194 None:intergenic 35.0%
!! CAAGTCAAAGTGTTGTCAAA+TGG - chr5_1:55175219-55175238 None:intergenic 35.0%
!!! CCAGAATACCCAATTTTTTG+TGG - chr5_1:55175262-55175281 None:intergenic 35.0%
CCACAAAAAATTGGGTATTC+TGG + chr5_1:55175259-55175278 Msa0737730:intron 35.0%
! ATTCTGGAGATTATAGCATC+TGG + chr5_1:55175275-55175294 Msa0737730:intron 35.0%
TCTCAATGGACATAAACACA+TGG + chr5_1:55175356-55175375 Msa0737730:intron 35.0%
AAGTAACAGAAGAACTAGGA+AGG + chr5_1:55175506-55175525 Msa0737730:intron 35.0%
! TTTCGCTAGTAATTTGAAGC+TGG + chr5_1:55176015-55176034 Msa0737730:intron 35.0%
! TTCGCTAGTAATTTGAAGCT+GGG + chr5_1:55176016-55176035 Msa0737730:intron 35.0%
!! TAATTTGAAGCTGGGGTTTT+TGG + chr5_1:55176024-55176043 Msa0737730:intron 35.0%
! TGGCCTAACTTATAGCTTTT+TGG + chr5_1:55176044-55176063 Msa0737730:intron 35.0%
AAAAGAATGACAGGCTCTTA+TGG - chr5_1:55176273-55176292 None:intergenic 35.0%
! CTTCCATGTTTTACTTGATG+TGG + chr5_1:55176343-55176362 Msa0737730:intron 35.0%
CTTCAAAGAGTTATGTCTCA+AGG + chr5_1:55176449-55176468 Msa0737730:intron 35.0%
CCTTTAACATACGCAGATAA+TGG - chr5_1:55176481-55176500 None:intergenic 35.0%
CCATTATCTGCGTATGTTAA+AGG + chr5_1:55176478-55176497 Msa0737730:intron 35.0%
ATCTGTAGTTTCTAAGTCTC+GGG - chr5_1:55176550-55176569 None:intergenic 35.0%
CATCTGTAGTTTCTAAGTCT+CGG - chr5_1:55176551-55176570 None:intergenic 35.0%
CAATTCAGACTTAATGACTC+TGG - chr5_1:55176618-55176637 None:intergenic 35.0%
TAGGAAACCATCTTATATGG+GGG - chr5_1:55176726-55176745 None:intergenic 35.0%
ACTTTAAACTTCCCAGTTCT+CGG + chr5_1:55176954-55176973 Msa0737730:three_prime_UTR 35.0%
CACAGTTGTTAAAATTCCGT+AGG + chr5_1:55177013-55177032 Msa0737730:three_prime_UTR 35.0%
! AGTAACTTGCAGAATACCTA+CGG - chr5_1:55177032-55177051 None:intergenic 35.0%
!!! TTTTTGCCACTTTCTTCTGT+TGG + chr5_1:55177078-55177097 Msa0737730:three_prime_UTR 35.0%
TACTTGAGTCTGTAGAAGTT+GGG + chr5_1:55177104-55177123 Msa0737730:three_prime_UTR 35.0%
TTGGGCAATTATGGAAAGAA+CGG + chr5_1:55177122-55177141 Msa0737730:three_prime_UTR 35.0%
AAGGCTCTTAATTAGACCAA+TGG - chr5_1:55177210-55177229 None:intergenic 35.0%
CAAATCCACAAAGGTAACAC+TGG + chr5_1:55172797-55172816 Msa0737730:intron 40.0%
!!! TGTTTTGCTTGTTTCTGGAC+TGG + chr5_1:55172946-55172965 Msa0737730:intron 40.0%
ACGCTGCAACATTACTAAGT+AGG - chr5_1:55173438-55173457 None:intergenic 40.0%
CTTCTTGTGGATATAGTTGG+TGG + chr5_1:55174043-55174062 Msa0737730:intron 40.0%
TTAGCCGAAGTTGTTGAACA+TGG + chr5_1:55174071-55174090 Msa0737730:CDS 40.0%
ATGCATCAGATTGCTGATCT+GGG - chr5_1:55174100-55174119 None:intergenic 40.0%
AATGCATCAGATTGCTGATC+TGG - chr5_1:55174101-55174120 None:intergenic 40.0%
AGATCAGCAATCTGATGCAT+TGG + chr5_1:55174100-55174119 Msa0737730:CDS 40.0%
TTCTATTAGCACCAAGGCTA+TGG + chr5_1:55174385-55174404 Msa0737730:CDS 40.0%
AGAGATAGATACCATAGCCT+TGG - chr5_1:55174399-55174418 None:intergenic 40.0%
!! TAGATAGAACTGGCCTTTGA+TGG - chr5_1:55174740-55174759 None:intergenic 40.0%
GCCAGTTCTATCTATACTCA+CGG + chr5_1:55174746-55174765 Msa0737730:intron 40.0%
CCAGTTCTATCTATACTCAC+GGG + chr5_1:55174747-55174766 Msa0737730:intron 40.0%
CACACTCTTCGTAATCATGA+TGG + chr5_1:55174798-55174817 Msa0737730:intron 40.0%
GATTCCAGTGTTGTCAATCT+CGG + chr5_1:55174850-55174869 Msa0737730:intron 40.0%
TATTCCGAGATTGACAACAC+TGG - chr5_1:55174857-55174876 None:intergenic 40.0%
GTGTTGTCAATCTCGGAATA+CGG + chr5_1:55174857-55174876 Msa0737730:intron 40.0%
CTGATATTCGATAACACAGG+CGG - chr5_1:55175002-55175021 None:intergenic 40.0%
TGGCTGATATTCGATAACAC+AGG - chr5_1:55175005-55175024 None:intergenic 40.0%
GTGTTATCGAATATCAGCCA+TGG + chr5_1:55175005-55175024 Msa0737730:intron 40.0%
TTATCGAATATCAGCCATGG+AGG + chr5_1:55175008-55175027 Msa0737730:intron 40.0%
! AGGAAATCAATGGCGGTTTT+TGG + chr5_1:55175028-55175047 Msa0737730:intron 40.0%
! GGAAATCAATGGCGGTTTTT+GGG + chr5_1:55175029-55175048 Msa0737730:intron 40.0%
ATGCCCAATTTCGACCAATA+TGG + chr5_1:55175061-55175080 Msa0737730:intron 40.0%
! TTTTCCGCCATAATCTGATG+TGG + chr5_1:55175089-55175108 Msa0737730:intron 40.0%
AGGCTCTCATAAATTTCTGC+TGG + chr5_1:55175460-55175479 Msa0737730:intron 40.0%
CATCTCAAAAGCTTCACTTC+TGG + chr5_1:55175860-55175879 Msa0737730:intron 40.0%
GAGGACAATCACATTGAGAA+AGG - chr5_1:55175974-55175993 None:intergenic 40.0%
! TCGCTAGTAATTTGAAGCTG+GGG + chr5_1:55176017-55176036 Msa0737730:intron 40.0%
TTGCTTAATCCAGACTGTTG+AGG + chr5_1:55176067-55176086 Msa0737730:intron 40.0%
TGCTTAATCCAGACTGTTGA+GGG + chr5_1:55176068-55176087 Msa0737730:intron 40.0%
ACCTGTCTGAAAAGAATGAC+AGG - chr5_1:55176282-55176301 None:intergenic 40.0%
TGACTCTGGATGTCTCAAAA+GGG - chr5_1:55176604-55176623 None:intergenic 40.0%
ATGACTCTGGATGTCTCAAA+AGG - chr5_1:55176605-55176624 None:intergenic 40.0%
AGGAAACCATCTTATATGGG+GGG - chr5_1:55176725-55176744 None:intergenic 40.0%
CTACTTGAGTCTGTAGAAGT+TGG + chr5_1:55177103-55177122 Msa0737730:three_prime_UTR 40.0%
CTGTAGAAGTTGGGCAATTA+TGG + chr5_1:55177113-55177132 Msa0737730:three_prime_UTR 40.0%
! TGACGAGAGGAGTACGAAAA+TGG - chr5_1:55172654-55172673 None:intergenic 45.0%
CAGGTATGCTCATTCTACTG+TGG + chr5_1:55172674-55172693 Msa0737730:intron 45.0%
ATCCACAAAGGTAACACTGG+TGG + chr5_1:55172800-55172819 Msa0737730:intron 45.0%
AGCAGGTGACTGCATAAAGT+TGG + chr5_1:55173061-55173080 Msa0737730:intron 45.0%
! ATGGAGCATTACTCTTCTCC+TGG + chr5_1:55173634-55173653 Msa0737730:CDS 45.0%
! GTTGTTGTTGTTGTTGCTGC+TGG - chr5_1:55173882-55173901 None:intergenic 45.0%
TGAAGATTCTGCTGTTGCTG+TGG - chr5_1:55173907-55173926 None:intergenic 45.0%
GCTGCTGACACTTCTCAAAA+TGG - chr5_1:55174346-55174365 None:intergenic 45.0%
CTCTGCTTCTATTAGCACCA+AGG + chr5_1:55174379-55174398 Msa0737730:CDS 45.0%
TGCACATGTGAGATGGCTTA+GGG + chr5_1:55174685-55174704 Msa0737730:intron 45.0%
GAGATGGCTTAGGGTAATGA+TGG + chr5_1:55174694-55174713 Msa0737730:intron 45.0%
CCCGTGAGTATAGATAGAAC+TGG - chr5_1:55174750-55174769 None:intergenic 45.0%
TCAGCCATGGAGGAAATCAA+TGG + chr5_1:55175018-55175037 Msa0737730:intron 45.0%
! TTGGTCGAAATTGGGCATTG+CGG - chr5_1:55175059-55175078 None:intergenic 45.0%
CCGCCATATTGGTCGAAATT+GGG - chr5_1:55175067-55175086 None:intergenic 45.0%
CCCAATTTCGACCAATATGG+CGG + chr5_1:55175064-55175083 Msa0737730:intron 45.0%
CCAATTTCGACCAATATGGC+GGG + chr5_1:55175065-55175084 Msa0737730:intron 45.0%
TCAGTGTTGTCTATGGCAGA+TGG - chr5_1:55175168-55175187 None:intergenic 45.0%
TTCAGGTCCTTCTAGTAACC+AGG + chr5_1:55175757-55175776 Msa0737730:intron 45.0%
TTAGGGTCCTGGTTACTAGA+AGG - chr5_1:55175767-55175786 None:intergenic 45.0%
TTCTAGTAACCAGGACCCTA+AGG + chr5_1:55175766-55175785 Msa0737730:intron 45.0%
TAACAAATGCCTTAGGGTCC+TGG - chr5_1:55175778-55175797 None:intergenic 45.0%
! TTTTCTGTCCCTCAACAGTC+TGG - chr5_1:55176079-55176098 None:intergenic 45.0%
TGAGGGACAGAAAAAGAAGC+TGG + chr5_1:55176085-55176104 Msa0737730:CDS 45.0%
! GCCTGTCATTCTTTTCAGAC+AGG + chr5_1:55176278-55176297 Msa0737730:three_prime_UTR 45.0%
GCGTATGTTAAAGGGTGAAC+AGG + chr5_1:55176487-55176506 Msa0737730:intron 45.0%
GTTAAAGGGTGAACAGGTGT+AGG + chr5_1:55176493-55176512 Msa0737730:intron 45.0%
GTAGTTTCTAAGTCTCGGGA+AGG - chr5_1:55176546-55176565 None:intergenic 45.0%
TATGGGGGGGGATTAAAAGA+CGG - chr5_1:55176711-55176730 None:intergenic 45.0%
TAATCCCCCCCCATATAAGA+TGG + chr5_1:55176716-55176735 Msa0737730:intron 45.0%
GAAACCATCTTATATGGGGG+GGG - chr5_1:55176723-55176742 None:intergenic 45.0%
GGAAACCATCTTATATGGGG+GGG - chr5_1:55176724-55176743 None:intergenic 45.0%
ACAAAAGATGCAGAGTCCAC+CGG + chr5_1:55176773-55176792 Msa0737730:intron 45.0%
CGAGCAAGATCTGCAATGTA+TGG - chr5_1:55176924-55176943 None:intergenic 45.0%
GTAGAGCCAACAGAAGAAAG+TGG - chr5_1:55177087-55177106 None:intergenic 45.0%
!! TTAAAAATTAAAAATTTGAA+AGG - chr5_1:55173531-55173550 None:intergenic 5.0%
!!! TATTTTCTTTAAAAAAAAAT+AGG + chr5_1:55177244-55177263 Msa0737730:three_prime_UTR 5.0%
TTTCGTACTCCTCTCGTCAC+AGG + chr5_1:55172655-55172674 Msa0737730:five_prime_UTR 50.0%
ATGAGCATACCTGTGACGAG+AGG - chr5_1:55172667-55172686 None:intergenic 50.0%
CACCACCAGTGTTACCTTTG+TGG - chr5_1:55172805-55172824 None:intergenic 50.0%
GAGGGATCGAATTGAAGCGA+AGG + chr5_1:55173596-55173615 Msa0737730:five_prime_UTR 50.0%
GAGCATTACTCTTCTCCTGG+TGG + chr5_1:55173637-55173656 Msa0737730:CDS 50.0%
ACTCTTCTCCTGGTGGAACA+TGG + chr5_1:55173644-55173663 Msa0737730:CDS 50.0%
ATCATCGTCCATGTTCCACC+AGG - chr5_1:55173655-55173674 None:intergenic 50.0%
GGGGATTGGAATTAGGGGTT+GGG - chr5_1:55173678-55173697 None:intergenic 50.0%
TGGGGATTGGAATTAGGGGT+TGG - chr5_1:55173679-55173698 None:intergenic 50.0%
AGATTCTGCTGTTGCTGTGG+AGG - chr5_1:55173904-55173923 None:intergenic 50.0%
TTTACCGGTGTAGACGTGTC+AGG + chr5_1:55174196-55174215 Msa0737730:intron 50.0%
TTACCGGTGTAGACGTGTCA+GGG + chr5_1:55174197-55174216 Msa0737730:intron 50.0%
CTAAGCCATCTCACATGTGC+AGG - chr5_1:55174686-55174705 None:intergenic 50.0%
CTGCACATGTGAGATGGCTT+AGG + chr5_1:55174684-55174703 Msa0737730:intron 50.0%
! GGCTTAGGGTAATGATGGAG+AGG + chr5_1:55174699-55174718 Msa0737730:intron 50.0%
! TACTCACGGGCCATCGATTA+TGG + chr5_1:55174760-55174779 Msa0737730:intron 50.0%
GCTATTGCGGCGTTATAGCT+CGG - chr5_1:55174916-55174935 None:intergenic 50.0%
GCCATGGAGGAAATCAATGG+CGG + chr5_1:55175021-55175040 Msa0737730:intron 50.0%
!! ACCGCCATTGATTTCCTCCA+TGG - chr5_1:55175025-55175044 None:intergenic 50.0%
CCCGCCATATTGGTCGAAAT+TGG - chr5_1:55175068-55175087 None:intergenic 50.0%
TCCGCCATAATCTGATGTGG+CGG + chr5_1:55175092-55175111 Msa0737730:intron 50.0%
GATCCAAAATCCCGCCATAC+CGG - chr5_1:55175131-55175150 None:intergenic 50.0%
CTATGGCAGATGGCAGTACA+TGG - chr5_1:55175158-55175177 None:intergenic 50.0%
GGGACAGAAAAAGAAGCTGG+AGG + chr5_1:55176088-55176107 Msa0737730:CDS 50.0%
ATGGGGGGGGATTAAAAGAC+GGG - chr5_1:55176710-55176729 None:intergenic 50.0%
!! GGTGGACTCTGCATCTTTTG+TGG - chr5_1:55176774-55176793 None:intergenic 50.0%
CGTGTGCTCTCATTGTATGC+CGG - chr5_1:55176795-55176814 None:intergenic 50.0%
CAAGCGATTCTCCGAGAACT+GGG - chr5_1:55176968-55176987 None:intergenic 50.0%
CAGTTCTCGGAGAATCGCTT+GGG + chr5_1:55176967-55176986 Msa0737730:three_prime_UTR 50.0%
!! GGGATCGATTTGGGCTTCAG+AGG - chr5_1:55172590-55172609 None:intergenic 55.0%
TGACCCTGACACGTCTACAC+CGG - chr5_1:55174203-55174222 None:intergenic 55.0%
ATCTCACATGTGCAGGCTCG+AGG - chr5_1:55174679-55174698 None:intergenic 55.0%
TCGAGCCTGCACATGTGAGA+TGG + chr5_1:55174678-55174697 Msa0737730:intron 55.0%
GGCGGAAAATCCCGCCATAT+TGG - chr5_1:55175078-55175097 None:intergenic 55.0%
GCCGCCACATCAGATTATGG+CGG - chr5_1:55175096-55175115 None:intergenic 55.0%
! GGTGCCGCCACATCAGATTA+TGG - chr5_1:55175099-55175118 None:intergenic 55.0%
TGGCAGATGGCAGTACATGG+CGG - chr5_1:55175155-55175174 None:intergenic 55.0%
GGGGGATTAAAAGACGGGTC+AGG - chr5_1:55176705-55176724 None:intergenic 55.0%
GTGCTCTCATTGTATGCCGG+TGG - chr5_1:55176792-55176811 None:intergenic 55.0%
CCAAGCGATTCTCCGAGAAC+TGG - chr5_1:55176969-55176988 None:intergenic 55.0%
CCAGTTCTCGGAGAATCGCT+TGG + chr5_1:55176966-55176985 Msa0737730:three_prime_UTR 55.0%
GATGTGGCGGCACCACAGAG+CGG + chr5_1:55175105-55175124 Msa0737730:intron 65.0%
!! CGGCCGGTATGGCGGGATTT+TGG + chr5_1:55175125-55175144 Msa0737730:intron 65.0%
GCACCACAGAGCGGCCGGTA+TGG + chr5_1:55175114-55175133 Msa0737730:intron 70.0%
CCACAGAGCGGCCGGTATGG+CGG + chr5_1:55175117-55175136 Msa0737730:intron 70.0%
CACAGAGCGGCCGGTATGGC+GGG + chr5_1:55175118-55175137 Msa0737730:intron 70.0%
TGGCGGCACCACAGAGCGGC+CGG + chr5_1:55175109-55175128 Msa0737730:intron 75.0%
CCGCCATACCGGCCGCTCTG+TGG - chr5_1:55175120-55175139 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 55172568 55177418 55172568 ID=Msa0737730;Name=Msa0737730
chr5_1 mRNA 55172568 55177418 55172568 ID=Msa0737730-mRNA-1;Parent=Msa0737730;Name=Msa0737730-mRNA-1;_AED=0.06;_eAED=0.06;_QI=153|1|1|1|0.66|0.42|7|509|195
chr5_1 exon 55172568 55172676 55172568 ID=Msa0737730-mRNA-1:exon:15522;Parent=Msa0737730-mRNA-1
chr5_1 exon 55173590 55173960 55173590 ID=Msa0737730-mRNA-1:exon:15523;Parent=Msa0737730-mRNA-1
chr5_1 exon 55174059 55174121 55174059 ID=Msa0737730-mRNA-1:exon:15524;Parent=Msa0737730-mRNA-1
chr5_1 exon 55174326 55174406 55174326 ID=Msa0737730-mRNA-1:exon:15525;Parent=Msa0737730-mRNA-1
chr5_1 exon 55176080 55176138 55176080 ID=Msa0737730-mRNA-1:exon:15526;Parent=Msa0737730-mRNA-1
chr5_1 exon 55176218 55176299 55176218 ID=Msa0737730-mRNA-1:exon:15527;Parent=Msa0737730-mRNA-1
chr5_1 exon 55176934 55177418 55176934 ID=Msa0737730-mRNA-1:exon:15528;Parent=Msa0737730-mRNA-1
chr5_1 five_prime_UTR 55172568 55172676 55172568 ID=Msa0737730-mRNA-1:five_prime_utr;Parent=Msa0737730-mRNA-1
chr5_1 five_prime_UTR 55173590 55173633 55173590 ID=Msa0737730-mRNA-1:five_prime_utr;Parent=Msa0737730-mRNA-1
chr5_1 CDS 55173634 55173960 55173634 ID=Msa0737730-mRNA-1:cds;Parent=Msa0737730-mRNA-1
chr5_1 CDS 55174059 55174121 55174059 ID=Msa0737730-mRNA-1:cds;Parent=Msa0737730-mRNA-1
chr5_1 CDS 55174326 55174406 55174326 ID=Msa0737730-mRNA-1:cds;Parent=Msa0737730-mRNA-1
chr5_1 CDS 55176080 55176138 55176080 ID=Msa0737730-mRNA-1:cds;Parent=Msa0737730-mRNA-1
chr5_1 CDS 55176218 55176275 55176218 ID=Msa0737730-mRNA-1:cds;Parent=Msa0737730-mRNA-1
chr5_1 three_prime_UTR 55176276 55176299 55176276 ID=Msa0737730-mRNA-1:three_prime_utr;Parent=Msa0737730-mRNA-1
chr5_1 three_prime_UTR 55176934 55177418 55176934 ID=Msa0737730-mRNA-1:three_prime_utr;Parent=Msa0737730-mRNA-1
Gene Sequence

>Msa0737730

ATGGAGCATTACTCTTCTCCTGGTGGAACATGGACGATGATCCCAACCCCTAATTCCAATCCCCAAATTCAATCTCAATCTCAATCTCAATCAAATCAAGACTCAAATCTTTATCTTCAACAGCAACAACAACAATTTCTTCAACAACAACAACAACCTTTTCAACAACCTCAAACAACACAATCTCAATTCCAACAACAACAACAACTTTATCAACAACAACAACAACAACGTATTCTGCAACACCAGCAGCAACAACAACAACAACCTCCACAGCAACAGCAGAATCTTCATCAATCACTCACTTCTCACTTTCATCTCTTAAACTTGGTGGAGAATTTAGCCGAAGTTGTTGAACATGGAAACCCAGATCAGCAATCTGATGCATTGATCACTGAACTGAGCAACCATTTTGAGAAGTGTCAGCAGCTGTTAAACTCAATCTCTGCTTCTATTAGCACCAAGGCTATGACTGTTGAGGGACAGAAAAAGAAGCTGGAGGAAAGCGAGCAATTGCTAAATCAAAGAAGAGATTTGATTTCCAATTACACAAAATCTGTAGAGGAGCTTGTCAGATCTGAGCCATAA
Protein sequence

>Msa0737730

MEHYSSPGGTWTMIPTPNSNPQIQSQSQSQSNQDSNLYLQQQQQQFLQQQQQPFQQPQTTQSQFQQQQQLYQQQQQQRILQHQQQQQQQPPQQQQNLHQSLTSHFHLLNLVENLAEVVEHGNPDQQSDALITELSNHFEKCQQLLNSISASISTKAMTVEGQKKKLEESEQLLNQRRDLISNYTKSVEELVRSEP*