AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0809770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0809770 MtrunA17_Chr5g0425531 95.288 191 9 0 1 191 595 785 2.88e-119 355
Msa0809770 MtrunA17_Chr5g0425471 95.288 191 9 0 1 191 592 782 2.13e-118 353
Msa0809770 MtrunA17_Chr5g0425451 94.241 191 11 0 1 191 592 782 2.09e-117 351
Msa0809770 MtrunA17_Chr5g0425431 93.194 191 13 0 1 191 590 780 8.60e-116 346
Msa0809770 MtrunA17_Chr3g0115601 64.398 191 68 0 1 191 586 776 2.30e-90 280
Msa0809770 MtrunA17_Chr5g0411571 39.899 198 110 2 1 191 587 782 3.11e-44 156
Msa0809770 MtrunA17_Chr2g0294751 37.766 188 105 4 2 187 575 752 4.60e-37 135
Msa0809770 MtrunA17_Chr2g0278761 35.751 193 114 4 1 191 591 775 2.73e-36 133
Msa0809770 MtrunA17_Chr1g0207281 35.577 208 108 5 1 192 594 791 5.69e-35 130
Msa0809770 MtrunA17_Chr4g0050591 35.354 198 108 5 1 191 590 774 9.28e-34 126
Msa0809770 MtrunA17_Chr1g0194841 34.848 198 107 5 1 189 571 755 1.04e-31 120
Msa0809770 MtrunA17_Chr7g0269871 35.354 198 107 7 1 189 576 761 5.37e-29 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0809770 AT1G78060.1 51.813 193 86 4 1 191 580 767 1.54e-62 206
Msa0809770 AT5G10560.1 38.021 192 115 1 2 189 599 790 5.37e-41 147
Msa0809770 AT5G09700.1 38.974 195 110 4 1 191 337 526 3.27e-34 127
Msa0809770 AT5G09730.1 38.071 197 109 5 1 191 584 773 1.24e-33 126
Msa0809770 AT3G19620.1 38.172 186 101 5 2 181 581 758 2.63e-32 122
Msa0809770 AT1G02640.1 35.263 190 113 4 1 189 578 758 4.41e-32 122
Msa0809770 AT5G64570.1 37.879 198 109 4 1 191 594 784 7.35e-32 121
Msa0809770 AT5G64570.2 37.879 198 109 4 1 191 436 626 8.28e-32 120
Msa0809770 AT5G49360.1 37.056 197 104 8 1 187 582 768 1.78e-31 120

Find 53 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCATTTCTACCTTTCTT+TGG 0.223144 5_3:+37015130 None:intergenic
TTTGACTGAAATGAAGTTGT+AGG 0.288846 5_3:+37015339 None:intergenic
TTTGAGAGTGTGACGGTAGA+AGG 0.295260 5_3:-37015085 Msa0809770:CDS
TACAAACTTGTTTCAGAGAT+AGG 0.351981 5_3:-37015250 Msa0809770:CDS
AGCAAATGAGAGTGGTGTTA+AGG 0.359751 5_3:-37015008 Msa0809770:CDS
GGAGGTGGAAAGGGTGAAGT+TGG 0.369858 5_3:-37015064 Msa0809770:CDS
ACATTGGGAATTACAAATAC+TGG 0.373679 5_3:-37015193 Msa0809770:CDS
GGTCCAAAGGTCTACGAATT+CGG 0.387773 5_3:-37015388 Msa0809770:CDS
ACAAATACTGGAGGCATGGC+TGG 0.398922 5_3:-37015181 Msa0809770:CDS
AGGAATAATTTGAGTAGCTT+AGG 0.401616 5_3:+37015359 None:intergenic
TAGCCGAATTCGTAGACCTT+TGG 0.407677 5_3:+37015385 None:intergenic
AACCCTACCAACTGTTTCAC+TGG 0.418928 5_3:+37015106 None:intergenic
AGGTGGTTATTTGTTGGTTG+TGG 0.420306 5_3:-37014975 Msa0809770:CDS
TCTCTGAAACAAGTTTGTAA+TGG 0.433264 5_3:+37015254 None:intergenic
AAGCCAAAGAAAGGTAGAAA+TGG 0.439382 5_3:-37015133 Msa0809770:CDS
AATTCCCAATGTGACAGAAA+TGG 0.462642 5_3:+37015204 None:intergenic
ATGTCCATTTCTGTCACATT+GGG 0.462961 5_3:-37015208 Msa0809770:CDS
CTCATTCTCATGTCAGTCAT+TGG 0.465198 5_3:+37015460 None:intergenic
TTATTTGTTGGTTGTGGGAG+AGG 0.471909 5_3:-37014969 Msa0809770:CDS
ACGGTAGAAGGAGGTGGAAA+GGG 0.488877 5_3:-37015073 Msa0809770:CDS
GGTGGTTATTTGTTGGTTGT+GGG 0.491601 5_3:-37014974 Msa0809770:CDS
CATGTCCATTTCTGTCACAT+TGG 0.509436 5_3:-37015209 Msa0809770:CDS
ATGAGGGCTGATCCATCATC+AGG 0.511303 5_3:-37015442 Msa0809770:CDS
GTAGAAACGGTAGGTTCTAC+CGG 0.511605 5_3:+37015414 None:intergenic
TACTCAATAAATATCACTGT+TGG 0.517582 5_3:-37014941 Msa0809770:CDS
CTACCGGGATAGCCTGATGA+TGG 0.517596 5_3:+37015430 None:intergenic
GACGGTAGAAGGAGGTGGAA+AGG 0.524657 5_3:-37015074 Msa0809770:CDS
TTGTTGATTGATTGATGTGA+AGG 0.527129 5_3:+37015308 None:intergenic
TGAAGAAGGTGGTTATTTGT+TGG 0.528173 5_3:-37014981 Msa0809770:CDS
AAGGTCTACGAATTCGGCTA+TGG 0.539382 5_3:-37015382 Msa0809770:CDS
CCTTTGGACCTGTGTAGAAA+CGG 0.540578 5_3:+37015401 None:intergenic
GATCCATCATCAGGCTATCC+CGG 0.540808 5_3:-37015433 Msa0809770:CDS
ATCCAGTGAAACAGTTGGTA+GGG 0.542668 5_3:-37015108 Msa0809770:CDS
TTGTTTGTGAAGCCAAAGAA+AGG 0.545721 5_3:-37015142 Msa0809770:CDS
AGTGTGACGGTAGAAGGAGG+TGG 0.547541 5_3:-37015079 Msa0809770:CDS
AATCCAGTGAAACAGTTGGT+AGG 0.561271 5_3:-37015109 Msa0809770:CDS
GAGAGTGTGACGGTAGAAGG+AGG 0.561859 5_3:-37015082 Msa0809770:CDS
CAAATACTGGAGGCATGGCT+GGG 0.567905 5_3:-37015180 Msa0809770:CDS
CAATGACTGACATGAGAATG+AGG 0.574292 5_3:-37015459 Msa0809770:CDS
GGTAGGGTTTGAGAGTGTGA+CGG 0.580407 5_3:-37015092 Msa0809770:CDS
AATTACAAATACTGGAGGCA+TGG 0.594236 5_3:-37015185 Msa0809770:CDS
CAATGTGACAGAAATGGACA+TGG 0.599069 5_3:+37015210 None:intergenic
GGTGTTAAGGTGATTGAAGA+AGG 0.603997 5_3:-37014995 Msa0809770:CDS
CCGTTTCTACACAGGTCCAA+AGG 0.609130 5_3:-37015401 Msa0809770:CDS
GGCTTCACAAACAACAACAC+AGG 0.609698 5_3:+37015151 None:intergenic
AGAACCTACCGTTTCTACAC+AGG 0.612051 5_3:-37015409 Msa0809770:CDS
GTTAAGGTGATTGAAGAAGG+TGG 0.615321 5_3:-37014992 Msa0809770:CDS
TGGAAATCCAGTGAAACAGT+TGG 0.620701 5_3:-37015113 Msa0809770:CDS
TGGACCTGTGTAGAAACGGT+AGG 0.625209 5_3:+37015405 None:intergenic
TAGAAACGGTAGGTTCTACC+GGG 0.648311 5_3:+37015415 None:intergenic
CTTAGTAGAGCAAATGAGAG+TGG 0.648348 5_3:-37015016 Msa0809770:CDS
AATGACTGACATGAGAATGA+GGG 0.678215 5_3:-37015458 Msa0809770:CDS
TTGGGAATTACAAATACTGG+AGG 0.696508 5_3:-37015190 Msa0809770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATTTTGATGAAATCTTTT+GGG + chr5_3:37014951-37014970 None:intergenic 20.0%
!!! GGTATTTTGATGAAATCTTT+TGG + chr5_3:37014952-37014971 None:intergenic 25.0%
! TACTCAATAAATATCACTGT+TGG - chr5_3:37015489-37015508 Msa0809770:CDS 25.0%
AGGAATAATTTGAGTAGCTT+AGG + chr5_3:37015074-37015093 None:intergenic 30.0%
TTTGACTGAAATGAAGTTGT+AGG + chr5_3:37015094-37015113 None:intergenic 30.0%
! TTGTTGATTGATTGATGTGA+AGG + chr5_3:37015125-37015144 None:intergenic 30.0%
!! TCTCTGAAACAAGTTTGTAA+TGG + chr5_3:37015179-37015198 None:intergenic 30.0%
! TACAAACTTGTTTCAGAGAT+AGG - chr5_3:37015180-37015199 Msa0809770:CDS 30.0%
ACATTGGGAATTACAAATAC+TGG - chr5_3:37015237-37015256 Msa0809770:CDS 30.0%
TTTCCATTTCTACCTTTCTT+TGG + chr5_3:37015303-37015322 None:intergenic 30.0%
AATGACTGACATGAGAATGA+GGG - chr5_3:37014972-37014991 Msa0809770:CDS 35.0%
ATGTCCATTTCTGTCACATT+GGG - chr5_3:37015222-37015241 Msa0809770:CDS 35.0%
AATTCCCAATGTGACAGAAA+TGG + chr5_3:37015229-37015248 None:intergenic 35.0%
TTGGGAATTACAAATACTGG+AGG - chr5_3:37015240-37015259 Msa0809770:CDS 35.0%
AATTACAAATACTGGAGGCA+TGG - chr5_3:37015245-37015264 Msa0809770:CDS 35.0%
TTGTTTGTGAAGCCAAAGAA+AGG - chr5_3:37015288-37015307 Msa0809770:CDS 35.0%
AAGCCAAAGAAAGGTAGAAA+TGG - chr5_3:37015297-37015316 Msa0809770:CDS 35.0%
TGAAGAAGGTGGTTATTTGT+TGG - chr5_3:37015449-37015468 Msa0809770:CDS 35.0%
CTCATTCTCATGTCAGTCAT+TGG + chr5_3:37014973-37014992 None:intergenic 40.0%
CAATGACTGACATGAGAATG+AGG - chr5_3:37014971-37014990 Msa0809770:CDS 40.0%
CAATGTGACAGAAATGGACA+TGG + chr5_3:37015223-37015242 None:intergenic 40.0%
CATGTCCATTTCTGTCACAT+TGG - chr5_3:37015221-37015240 Msa0809770:CDS 40.0%
TGGAAATCCAGTGAAACAGT+TGG - chr5_3:37015317-37015336 Msa0809770:CDS 40.0%
AATCCAGTGAAACAGTTGGT+AGG - chr5_3:37015321-37015340 Msa0809770:CDS 40.0%
! ATCCAGTGAAACAGTTGGTA+GGG - chr5_3:37015322-37015341 Msa0809770:CDS 40.0%
CTTAGTAGAGCAAATGAGAG+TGG - chr5_3:37015414-37015433 Msa0809770:CDS 40.0%
! AGCAAATGAGAGTGGTGTTA+AGG - chr5_3:37015422-37015441 Msa0809770:CDS 40.0%
GGTGTTAAGGTGATTGAAGA+AGG - chr5_3:37015435-37015454 Msa0809770:CDS 40.0%
GTTAAGGTGATTGAAGAAGG+TGG - chr5_3:37015438-37015457 Msa0809770:CDS 40.0%
! AGGTGGTTATTTGTTGGTTG+TGG - chr5_3:37015455-37015474 Msa0809770:CDS 40.0%
! GGTGGTTATTTGTTGGTTGT+GGG - chr5_3:37015456-37015475 Msa0809770:CDS 40.0%
! TTATTTGTTGGTTGTGGGAG+AGG - chr5_3:37015461-37015480 Msa0809770:CDS 40.0%
TAGAAACGGTAGGTTCTACC+GGG + chr5_3:37015018-37015037 None:intergenic 45.0%
GTAGAAACGGTAGGTTCTAC+CGG + chr5_3:37015019-37015038 None:intergenic 45.0%
AGAACCTACCGTTTCTACAC+AGG - chr5_3:37015021-37015040 Msa0809770:CDS 45.0%
CCTTTGGACCTGTGTAGAAA+CGG + chr5_3:37015032-37015051 None:intergenic 45.0%
GGTCCAAAGGTCTACGAATT+CGG - chr5_3:37015042-37015061 Msa0809770:CDS 45.0%
TAGCCGAATTCGTAGACCTT+TGG + chr5_3:37015048-37015067 None:intergenic 45.0%
AAGGTCTACGAATTCGGCTA+TGG - chr5_3:37015048-37015067 Msa0809770:CDS 45.0%
GGCTTCACAAACAACAACAC+AGG + chr5_3:37015282-37015301 None:intergenic 45.0%
AACCCTACCAACTGTTTCAC+TGG + chr5_3:37015327-37015346 None:intergenic 45.0%
! TTTGAGAGTGTGACGGTAGA+AGG - chr5_3:37015345-37015364 Msa0809770:CDS 45.0%
ATGAGGGCTGATCCATCATC+AGG - chr5_3:37014988-37015007 Msa0809770:CDS 50.0%
GATCCATCATCAGGCTATCC+CGG - chr5_3:37014997-37015016 Msa0809770:CDS 50.0%
TGGACCTGTGTAGAAACGGT+AGG + chr5_3:37015028-37015047 None:intergenic 50.0%
CCGTTTCTACACAGGTCCAA+AGG - chr5_3:37015029-37015048 Msa0809770:CDS 50.0%
! ACAAATACTGGAGGCATGGC+TGG - chr5_3:37015249-37015268 Msa0809770:CDS 50.0%
! CAAATACTGGAGGCATGGCT+GGG - chr5_3:37015250-37015269 Msa0809770:CDS 50.0%
! GGTAGGGTTTGAGAGTGTGA+CGG - chr5_3:37015338-37015357 Msa0809770:CDS 50.0%
ACGGTAGAAGGAGGTGGAAA+GGG - chr5_3:37015357-37015376 Msa0809770:CDS 50.0%
CTACCGGGATAGCCTGATGA+TGG + chr5_3:37015003-37015022 None:intergenic 55.0%
! GAGAGTGTGACGGTAGAAGG+AGG - chr5_3:37015348-37015367 Msa0809770:CDS 55.0%
AGTGTGACGGTAGAAGGAGG+TGG - chr5_3:37015351-37015370 Msa0809770:CDS 55.0%
GACGGTAGAAGGAGGTGGAA+AGG - chr5_3:37015356-37015375 Msa0809770:CDS 55.0%
GGAGGTGGAAAGGGTGAAGT+TGG - chr5_3:37015366-37015385 Msa0809770:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 37014937 37015515 37014937 ID=Msa0809770;Name=Msa0809770
chr5_3 mRNA 37014937 37015515 37014937 ID=Msa0809770-mRNA-1;Parent=Msa0809770;Name=Msa0809770-mRNA-1;_AED=0.46;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|192
chr5_3 exon 37014937 37015515 37014937 ID=Msa0809770-mRNA-1:exon:11507;Parent=Msa0809770-mRNA-1
chr5_3 CDS 37014937 37015515 37014937 ID=Msa0809770-mRNA-1:cds;Parent=Msa0809770-mRNA-1
Gene Sequence

>Msa0809770

ATGACATGGTACCCAAAAGATTTCATCAAAATACCAATGACTGACATGAGAATGAGGGCTGATCCATCATCAGGCTATCCCGGTAGAACCTACCGTTTCTACACAGGTCCAAAGGTCTACGAATTCGGCTATGGCCTAAGCTACTCAAATTATTCCTACAACTTCATTTCAGTCAAAAATAACAACCTTCACATCAATCAATCAACAACATATTCGATACTCGAAAAATCTCATACAATCCATTACAAACTTGTTTCAGAGATAGGCGAAAAAACATGTAAAACCATGTCCATTTCTGTCACATTGGGAATTACAAATACTGGAGGCATGGCTGGGAAACATCCTGTGTTGTTGTTTGTGAAGCCAAAGAAAGGTAGAAATGGAAATCCAGTGAAACAGTTGGTAGGGTTTGAGAGTGTGACGGTAGAAGGAGGTGGAAAGGGTGAAGTTGGATTCGAAGTTAGTGTTTGTGAGCATCTTAGTAGAGCAAATGAGAGTGGTGTTAAGGTGATTGAAGAAGGTGGTTATTTGTTGGTTGTGGGAGAGGAAGAGTACTCAATAAATATCACTGTTGGATGA

Protein sequence

>Msa0809770

MTWYPKDFIKIPMTDMRMRADPSSGYPGRTYRFYTGPKVYEFGYGLSYSNYSYNFISVKNNNLHINQSTTYSILEKSHTIHYKLVSEIGEKTCKTMSISVTLGITNTGGMAGKHPVLLFVKPKKGRNGNPVKQLVGFESVTVEGGGKGEVGFEVSVCEHLSRANESGVKVIEEGGYLLVVGEEEYSINITVG*