Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0313760 | XP_003593755.1 | 69.841 | 315 | 91 | 3 | 1 | 314 | 1 | 312 | 1.08e-142 | 415 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0313760 | sp|Q9FVS1|FBK23_ARATH | 29.500 | 200 | 129 | 6 | 6 | 197 | 2 | 197 | 1.30e-14 | 77.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016570 | Msa0313760 | 0.807629 | 4.603119e-50 | -8.615850e-47 |
| Msa0102070 | Msa0313760 | 0.817346 | 3.459453e-52 | -8.615850e-47 |
| Msa0107580 | Msa0313760 | 0.801143 | 1.034883e-48 | -8.615850e-47 |
| Msa0111510 | Msa0313760 | 0.831183 | 1.928950e-55 | -8.615850e-47 |
| Msa0123810 | Msa0313760 | 0.809514 | 1.822669e-50 | -8.615850e-47 |
| Msa0142220 | Msa0313760 | 0.813877 | 2.049238e-51 | -8.615850e-47 |
| Msa0145750 | Msa0313760 | 0.837760 | 4.298117e-57 | -8.615850e-47 |
| Msa0153910 | Msa0313760 | 0.839034 | 2.016611e-57 | -8.615850e-47 |
| Msa0159720 | Msa0313760 | 0.805005 | 1.645014e-49 | -8.615850e-47 |
| Msa0165190 | Msa0313760 | 0.801705 | 7.936647e-49 | -8.615850e-47 |
| Msa0188460 | Msa0313760 | 0.825389 | 4.815581e-54 | -8.615850e-47 |
| Msa0192970 | Msa0313760 | 0.858085 | 1.049513e-62 | -8.615850e-47 |
| Msa0227480 | Msa0313760 | 0.819873 | 9.241153e-53 | -8.615850e-47 |
| Msa0231000 | Msa0313760 | 0.838151 | 3.410570e-57 | -8.615850e-47 |
| Msa0235140 | Msa0313760 | 0.940600 | 1.767397e-100 | -8.615850e-47 |
| Msa0267000 | Msa0313760 | 0.843830 | 1.101007e-58 | -8.615850e-47 |
| Msa0267010 | Msa0313760 | 0.835724 | 1.420535e-56 | -8.615850e-47 |
| Msa0270520 | Msa0313760 | 0.854729 | 1.013994e-61 | -8.615850e-47 |
| Msa0309020 | Msa0313760 | 0.846749 | 1.786555e-59 | -8.615850e-47 |
| Msa0313760 | Msa0337900 | 0.807745 | 4.349695e-50 | -8.615850e-47 |
| Msa0313760 | Msa0342240 | 0.835722 | 1.422005e-56 | -8.615850e-47 |
| Msa0313760 | Msa0355220 | 0.805302 | 1.425377e-49 | -8.615850e-47 |
| Msa0313760 | Msa0360510 | 0.843527 | 1.326728e-58 | -8.615850e-47 |
| Msa0313760 | Msa0365500 | 0.811256 | 7.668276e-51 | -8.615850e-47 |
| Msa0313760 | Msa0366580 | 0.805867 | 1.084987e-49 | -8.615850e-47 |
| Msa0313760 | Msa0384800 | 0.868074 | 8.555950e-66 | -8.615850e-47 |
| Msa0313760 | Msa0394970 | 0.807971 | 3.894699e-50 | -8.615850e-47 |
| Msa0313760 | Msa0402290 | 0.810885 | 9.228107e-51 | -8.615850e-47 |
| Msa0313760 | Msa0406120 | 0.814727 | 1.329638e-51 | -8.615850e-47 |
| Msa0313760 | Msa0414410 | 0.858854 | 6.187732e-63 | -8.615850e-47 |
| Msa0313760 | Msa0414420 | 0.865117 | 7.449484e-65 | -8.615850e-47 |
| Msa0313760 | Msa0423020 | 0.803912 | 2.779098e-49 | -8.615850e-47 |
| Msa0313760 | Msa0436780 | 0.811333 | 7.379141e-51 | -8.615850e-47 |
| Msa0313760 | Msa0436800 | 0.813113 | 3.016579e-51 | -8.615850e-47 |
| Msa0313760 | Msa0438620 | 0.805815 | 1.112347e-49 | -8.615850e-47 |
| Msa0313760 | Msa0450000 | 0.824583 | 7.462554e-54 | -8.615850e-47 |
| Msa0313760 | Msa0452900 | 0.850904 | 1.256013e-60 | -8.615850e-47 |
| Msa0313760 | Msa0453120 | 0.814364 | 1.599655e-51 | -8.615850e-47 |
| Msa0313760 | Msa0454910 | 0.816744 | 4.722330e-52 | -8.615850e-47 |
| Msa0313760 | Msa0459160 | 0.856846 | 2.441248e-62 | -8.615850e-47 |
| Msa0313760 | Msa0459170 | 0.857693 | 1.371809e-62 | -8.615850e-47 |
| Msa0313760 | Msa0479340 | 0.801011 | 1.101243e-48 | -8.615850e-47 |
| Msa0313760 | Msa0506270 | 0.808673 | 2.759818e-50 | -8.615850e-47 |
| Msa0313760 | Msa0564890 | 0.878201 | 3.402402e-69 | -8.615850e-47 |
| Msa0313760 | Msa0596150 | 0.804030 | 2.627125e-49 | -8.615850e-47 |
| Msa0313760 | Msa0598780 | 0.831412 | 1.694321e-55 | -8.615850e-47 |
| Msa0313760 | Msa0636170 | 0.800727 | 1.258145e-48 | -8.615850e-47 |
| Msa0313760 | Msa0678930 | 0.842948 | 1.892602e-58 | -8.615850e-47 |
| Msa0313760 | Msa0694370 | 0.826701 | 2.348000e-54 | -8.615850e-47 |
| Msa0313760 | Msa0697060 | 0.803450 | 3.466210e-49 | -8.615850e-47 |
| Msa0313760 | Msa0726140 | 0.877081 | 8.364701e-69 | -8.615850e-47 |
| Msa0313760 | Msa0743810 | 0.844884 | 5.731612e-59 | -8.615850e-47 |
| Msa0313760 | Msa0745060 | 0.833335 | 5.658883e-56 | -8.615850e-47 |
| Msa0313760 | Msa0766630 | 0.820840 | 5.544700e-53 | -8.615850e-47 |
| Msa0313760 | Msa0767720 | 0.817511 | 3.175714e-52 | -8.615850e-47 |
| Msa0313760 | Msa0783160 | 0.846753 | 1.782037e-59 | -8.615850e-47 |
| Msa0313760 | Msa0787600 | 0.843087 | 1.737978e-58 | -8.615850e-47 |
| Msa0313760 | Msa0789250 | 0.804109 | 2.529338e-49 | -8.615850e-47 |
| Msa0313760 | Msa0808010 | 0.806645 | 7.440389e-50 | -8.615850e-47 |
| Msa0313760 | Msa0811080 | 0.802437 | 5.613418e-49 | -8.615850e-47 |
| Msa0313760 | Msa0825860 | 0.841458 | 4.693591e-58 | -8.615850e-47 |
| Msa0313760 | Msa0833290 | 0.802747 | 4.844666e-49 | -8.615850e-47 |
| Msa0313760 | Msa0846210 | 0.831984 | 1.224636e-55 | -8.615850e-47 |
| Msa0313760 | Msa0846330 | 0.828263 | 9.907220e-55 | -8.615850e-47 |
| Msa0313760 | Msa0846350 | 0.842271 | 2.863748e-58 | -8.615850e-47 |
| Msa0313760 | Msa0865320 | 0.818243 | 2.169935e-52 | -8.615850e-47 |
| Msa0313760 | Msa0885280 | 0.844512 | 7.221995e-59 | -8.615850e-47 |
| Msa0313760 | Msa0885290 | 0.860873 | 1.524153e-63 | -8.615850e-47 |
| Msa0313760 | Msa0887020 | 0.806330 | 8.666600e-50 | -8.615850e-47 |
| Msa0313760 | Msa0887990 | 0.816650 | 4.956908e-52 | -8.615850e-47 |
| Msa0313760 | Msa0888370 | 0.810809 | 9.582011e-51 | -8.615850e-47 |
| Msa0313760 | Msa0907260 | 0.813624 | 2.329423e-51 | -8.615850e-47 |
| Msa0313760 | Msa0922720 | 0.811414 | 7.084320e-51 | -8.615850e-47 |
| Msa0313760 | Msa0935910 | 0.811788 | 5.876171e-51 | -8.615850e-47 |
| Msa0313760 | Msa0954910 | 0.816295 | 5.953371e-52 | -8.615850e-47 |
| Msa0313760 | Msa0956270 | 0.809479 | 1.854402e-50 | -8.615850e-47 |
| Msa0313760 | Msa0981370 | 0.831416 | 1.690203e-55 | -8.615850e-47 |
| Msa0313760 | Msa0989060 | 0.828364 | 9.369265e-55 | -8.615850e-47 |
| Msa0313760 | Msa0998690 | 0.814312 | 1.642849e-51 | -8.615850e-47 |
| Msa0313760 | Msa1016280 | 0.817802 | 2.730067e-52 | -8.615850e-47 |
| Msa0313760 | Msa1023010 | 0.804285 | 2.324416e-49 | -8.615850e-47 |
| Msa0313760 | Msa1024240 | 0.815998 | 6.934575e-52 | -8.615850e-47 |
| Msa0313760 | Msa1025360 | 0.846734 | 1.803247e-59 | -8.615850e-47 |
| Msa0313760 | Msa1025370 | 0.841886 | 3.620466e-58 | -8.615850e-47 |
| Msa0313760 | Msa1044830 | 0.847334 | 1.234636e-59 | -8.615850e-47 |
| Msa0313760 | Msa1044840 | 0.850176 | 2.010984e-60 | -8.615850e-47 |
| Msa0313760 | Msa1060840 | 0.807460 | 5.001796e-50 | -8.615850e-47 |
| Msa0313760 | Msa1092990 | 0.818168 | 2.256298e-52 | -8.615850e-47 |
| Msa0313760 | Msa1100980 | 0.834508 | 2.877624e-56 | -8.615850e-47 |
| Msa0313760 | Msa1109440 | 0.836216 | 1.065450e-56 | -8.615850e-47 |
| Msa0313760 | Msa1121200 | 0.829434 | 5.158661e-55 | -8.615850e-47 |
| Msa0313760 | Msa1135740 | 0.828396 | 9.204834e-55 | -8.615850e-47 |
| Msa0313760 | Msa1136090 | 0.868565 | 5.943083e-66 | -8.615850e-47 |
| Msa0313760 | Msa1149360 | 0.808667 | 2.767826e-50 | -8.615850e-47 |
| Msa0313760 | Msa1150370 | 0.834526 | 2.848949e-56 | -8.615850e-47 |
| Msa0313760 | Msa1160670 | 0.832951 | 7.049485e-56 | -8.615850e-47 |
| Msa0313760 | Msa1173920 | 0.814718 | 1.335443e-51 | -8.615850e-47 |
| Msa0313760 | Msa1187940 | 0.823323 | 1.473578e-53 | -8.615850e-47 |
| Msa0313760 | Msa1187950 | 0.829182 | 5.940563e-55 | -8.615850e-47 |
| Msa0313760 | Msa1192640 | 0.858526 | 7.756175e-63 | -8.615850e-47 |
| Msa0313760 | Msa1260100 | 0.800805 | 1.213346e-48 | -8.615850e-47 |
| Msa0313760 | Msa1269670 | 0.832985 | 6.915140e-56 | -8.615850e-47 |
| Msa0313760 | Msa1270230 | 0.841924 | 3.536172e-58 | -8.615850e-47 |
| Msa0313760 | Msa1270240 | 0.826493 | 2.632455e-54 | -8.615850e-47 |
| Msa0313760 | Msa1270270 | 0.803242 | 3.828173e-49 | -8.615850e-47 |
| Msa0313760 | Msa1283330 | 0.836917 | 7.065636e-57 | -8.615850e-47 |
| Msa0313760 | Msa1285930 | 0.875530 | 2.866328e-68 | -8.615850e-47 |
| Msa0313760 | Msa1287800 | 0.853614 | 2.126860e-61 | -8.615850e-47 |
| Msa0313760 | Msa1287810 | 0.854584 | 1.116417e-61 | -8.615850e-47 |
| Msa0313760 | Msa1287820 | 0.816802 | 4.583419e-52 | -8.615850e-47 |
| Msa0313760 | Msa1287830 | 0.837174 | 6.072485e-57 | -8.615850e-47 |
| Msa0313760 | Msa1289430 | 0.807085 | 6.004809e-50 | -8.615850e-47 |
| Msa0313760 | Msa1297210 | 0.808042 | 3.760981e-50 | -8.615850e-47 |
| Msa0313760 | Msa1307890 | 0.818759 | 1.657578e-52 | -8.615850e-47 |
| Msa0313760 | Msa1320300 | 0.804148 | 2.482244e-49 | -8.615850e-47 |
| Msa0313760 | Msa1342200 | 0.800848 | 1.188927e-48 | -8.615850e-47 |
| Msa0313760 | Msa1369430 | 0.815374 | 9.550643e-52 | -8.615850e-47 |
| Msa0313760 | Msa1397580 | 0.804213 | 2.406165e-49 | -8.615850e-47 |
| Msa0313760 | Msa1397610 | 0.800343 | 1.506788e-48 | -8.615850e-47 |
| Msa0313760 | Msa1404000 | 0.852798 | 3.644522e-61 | -8.615850e-47 |
| Msa0313760 | Msa1415310 | 0.815461 | 9.137413e-52 | -8.615850e-47 |
| Msa0313760 | Msa1422180 | 0.840445 | 8.657204e-58 | -8.615850e-47 |
| Msa0313760 | Msa1433120 | 0.821773 | 3.378227e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0313760 | MtrunA17_Chr2g0283611 | 69.841 | 315 | 91 | 3 | 1 | 314 | 1 | 312 | 9.92e-147 | 415 |
| Msa0313760 | MtrunA17_Chr2g0283601 | 89.130 | 46 | 5 | 0 | 1 | 46 | 1 | 46 | 1.33e-21 | 87.0 |
| Msa0313760 | MtrunA17_Chr2g0283601 | 75.556 | 45 | 11 | 0 | 270 | 314 | 42 | 86 | 1.73e-17 | 76.3 |
| Msa0313760 | MtrunA17_Chr1g0178491 | 33.742 | 163 | 99 | 4 | 17 | 176 | 36 | 192 | 1.64e-21 | 93.6 |
| Msa0313760 | MtrunA17_Chr7g0266441 | 28.402 | 169 | 106 | 4 | 17 | 176 | 32 | 194 | 1.28e-13 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0313760 | AT1G57790.1 | 29.500 | 200 | 129 | 6 | 6 | 197 | 2 | 197 | 1.32e-15 | 77.0 |
| Msa0313760 | AT4G10660.1 | 30.909 | 165 | 97 | 6 | 82 | 237 | 38 | 194 | 1.67e-15 | 76.3 |
| Msa0313760 | AT4G10695.2 | 25.217 | 230 | 152 | 8 | 43 | 262 | 15 | 234 | 3.06e-15 | 74.3 |
| Msa0313760 | AT4G10695.1 | 25.217 | 230 | 152 | 8 | 43 | 262 | 15 | 234 | 3.06e-15 | 74.3 |
| Msa0313760 | AT1G49360.1 | 32.328 | 232 | 128 | 13 | 9 | 222 | 100 | 320 | 5.92e-15 | 75.5 |
| Msa0313760 | AT1G49360.5 | 32.328 | 232 | 128 | 13 | 9 | 222 | 77 | 297 | 6.02e-15 | 75.5 |
| Msa0313760 | AT3G18720.1 | 28.520 | 277 | 173 | 15 | 13 | 271 | 44 | 313 | 8.44e-15 | 74.7 |
| Msa0313760 | AT1G49360.3 | 32.579 | 221 | 120 | 13 | 20 | 222 | 8 | 217 | 1.09e-14 | 74.3 |
| Msa0313760 | AT1G49360.2 | 32.579 | 221 | 120 | 13 | 20 | 222 | 8 | 217 | 1.09e-14 | 74.3 |
| Msa0313760 | AT1G49360.4 | 32.579 | 221 | 120 | 13 | 20 | 222 | 8 | 217 | 1.09e-14 | 74.3 |
| Msa0313760 | AT3G56470.1 | 28.022 | 182 | 122 | 5 | 19 | 196 | 31 | 207 | 2.67e-13 | 70.1 |
| Msa0313760 | AT4G12370.1 | 44.444 | 90 | 44 | 4 | 83 | 170 | 18 | 103 | 3.10e-13 | 69.3 |
| Msa0313760 | AT4G00893.1 | 31.034 | 203 | 114 | 9 | 10 | 197 | 38 | 229 | 1.50e-12 | 68.2 |
| Msa0313760 | AT4G12382.1 | 33.333 | 129 | 74 | 4 | 8 | 130 | 1 | 123 | 4.02e-11 | 60.5 |
| Msa0313760 | AT4G12382.2 | 33.333 | 129 | 74 | 4 | 8 | 130 | 1 | 123 | 4.02e-11 | 60.5 |
Find 64 sgRNAs with CRISPR-Local
Find 140 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGATGATCATCTCCTTAA+AGG | 0.136143 | 2_4:+9214731 | Msa0313760:CDS |
| GCACGATCCATACTCCTTTA+AGG | 0.163291 | 2_4:-9214745 | None:intergenic |
| ATAATTTGTTTCTCTTTAAA+AGG | 0.183643 | 2_4:+9214399 | Msa0313760:CDS |
| TATATTCATGGTGGTAAAAT+AGG | 0.210484 | 2_4:-9214379 | None:intergenic |
| ATGGAGCGAGTGTAGTTTAT+TGG | 0.249967 | 2_4:+9214502 | Msa0313760:CDS |
| ACTCTATGATGCTCAAAATT+TGG | 0.258194 | 2_4:+9214676 | Msa0313760:CDS |
| CAAGTTGACCCAGCCAGTTC+TGG | 0.303744 | 2_4:-9214113 | None:intergenic |
| AGAGAAACAAATTATATTCA+TGG | 0.329456 | 2_4:-9214391 | None:intergenic |
| GATTCTGTGATAGGACAATT+TGG | 0.336565 | 2_4:-9214224 | None:intergenic |
| ATCATCTCCTTAAAGGAGTA+TGG | 0.340645 | 2_4:+9214738 | Msa0313760:CDS |
| TCTTAGTGTTTATGATATCT+TGG | 0.361386 | 2_4:-9214767 | None:intergenic |
| TGCTGTCAAATATTGCAAAT+AGG | 0.363050 | 2_4:+9213913 | Msa0313760:CDS |
| GCGCTCTGCGACTGAACCTT+TGG | 0.370991 | 2_4:+9213983 | Msa0313760:CDS |
| TTGGTTGACGCTAAATTTAG+TGG | 0.396967 | 2_4:+9214002 | Msa0313760:CDS |
| TTCATCTGCTCCCACGTCTC+AGG | 0.396997 | 2_4:+9214268 | Msa0313760:CDS |
| CAGTCATTCGATTCTGTGAT+AGG | 0.398376 | 2_4:-9214233 | None:intergenic |
| ACTACACTCGCTCCATTGTT+TGG | 0.401720 | 2_4:-9214495 | None:intergenic |
| TTCATCATGTGTGCAAAGAT+TGG | 0.403215 | 2_4:+9213961 | Msa0313760:CDS |
| TATAACTACAAGTTTATTGC+TGG | 0.411591 | 2_4:-9214456 | None:intergenic |
| TGAAAGGAAAACTAGGACTT+GGG | 0.413009 | 2_4:+9214624 | Msa0313760:CDS |
| CACTATTGAAATTCCAGAAC+TGG | 0.420244 | 2_4:+9214100 | Msa0313760:CDS |
| TTTGTCGGGGAGATAATTCA+TGG | 0.421163 | 2_4:+9214348 | Msa0313760:CDS |
| AGAACTGCACACGCTTTGTC+GGG | 0.421785 | 2_4:+9214334 | Msa0313760:CDS |
| CTGAAAGGAAAACTAGGACT+TGG | 0.430022 | 2_4:+9214623 | Msa0313760:CDS |
| TGGTGTGGGGTGATTACATT+TGG | 0.434879 | 2_4:+9214696 | Msa0313760:CDS |
| GATCCATGGTTTCTGTTGTA+TGG | 0.439332 | 2_4:+9214029 | Msa0313760:CDS |
| GTCAAATATTGCAAATAGGC+TGG | 0.448250 | 2_4:+9213917 | Msa0313760:CDS |
| AGGTGTGCATTCTTTATTGA+AGG | 0.450535 | 2_4:+9214419 | Msa0313760:CDS |
| TAGAACTGCACACGCTTTGT+CGG | 0.462441 | 2_4:+9214333 | Msa0313760:CDS |
| GCCTTGCATCCTACGACGGA+TGG | 0.482267 | 2_4:+9214135 | Msa0313760:CDS |
| TTTATCATCCTTGTTTAGCA+TGG | 0.488027 | 2_4:-9214072 | None:intergenic |
| ATATTTGACAGCAGATCATA+AGG | 0.498174 | 2_4:-9213903 | None:intergenic |
| AGTATTGAAATGTAGATTGA+GGG | 0.511372 | 2_4:+9214553 | Msa0313760:CDS |
| CAGTTCTAATTTATATTGAG+TGG | 0.524994 | 2_4:-9214318 | None:intergenic |
| GGTGTGCATTCTTTATTGAA+GGG | 0.534190 | 2_4:+9214420 | Msa0313760:CDS |
| CAGTATTGAAATGTAGATTG+AGG | 0.535602 | 2_4:+9214552 | Msa0313760:CDS |
| ATGTTGCAATAGATACTTGT+GGG | 0.537656 | 2_4:+9214651 | Msa0313760:CDS |
| ATTGAAATTCCAGAACTGGC+TGG | 0.538308 | 2_4:+9214104 | Msa0313760:CDS |
| AAGGATCTGAAAGGAAAACT+AGG | 0.543003 | 2_4:+9214617 | Msa0313760:CDS |
| TTGAAATTCCAGAACTGGCT+GGG | 0.548029 | 2_4:+9214105 | Msa0313760:CDS |
| AATTTAGTGGATGTGATCCA+TGG | 0.549686 | 2_4:+9214015 | Msa0313760:CDS |
| GTGTGCATTCTTTATTGAAG+GGG | 0.552789 | 2_4:+9214421 | Msa0313760:CDS |
| GCCATCCGTCGTAGGATGCA+AGG | 0.563825 | 2_4:-9214136 | None:intergenic |
| TGATGCTCAAAATTTGGTGT+GGG | 0.569401 | 2_4:+9214682 | Msa0313760:CDS |
| TTTCTGTTGTATGGTGAAGA+AGG | 0.575330 | 2_4:+9214038 | Msa0313760:CDS |
| AAAGGGAAAGCATCGATCAA+AGG | 0.578404 | 2_4:+9214598 | Msa0313760:CDS |
| ATGATGCTCAAAATTTGGTG+TGG | 0.583531 | 2_4:+9214681 | Msa0313760:CDS |
| CTAAATTTAGCGTCAACCAA+AGG | 0.595406 | 2_4:-9213999 | None:intergenic |
| GAGAAGCAGCCATCCGTCGT+AGG | 0.605702 | 2_4:-9214144 | None:intergenic |
| ACAATGCAATCCTGAGACGT+GGG | 0.606280 | 2_4:-9214278 | None:intergenic |
| AACAATGCAATCCTGAGACG+TGG | 0.609541 | 2_4:-9214279 | None:intergenic |
| TCGTTGTTCCATGCTAAACA+AGG | 0.611298 | 2_4:+9214064 | Msa0313760:CDS |
| TTGAGTGGCGTGATTAATCA+CGG | 0.617271 | 2_4:-9214303 | None:intergenic |
| TCACCATACAACAGAAACCA+TGG | 0.617900 | 2_4:-9214032 | None:intergenic |
| CATCGATCAAAGGATCTGAA+AGG | 0.623852 | 2_4:+9214608 | Msa0313760:CDS |
| AAAAGAAAAGCAAAGGAGAG+TGG | 0.624016 | 2_4:+9213871 | Msa0313760:CDS |
| ACTTGCCTTGCATCCTACGA+CGG | 0.635214 | 2_4:+9214131 | Msa0313760:CDS |
| GAACTGCACACGCTTTGTCG+GGG | 0.640853 | 2_4:+9214335 | Msa0313760:CDS |
| GAAAGGAAAACTAGGACTTG+GGG | 0.666358 | 2_4:+9214625 | Msa0313760:CDS |
| AATGTTGCAATAGATACTTG+TGG | 0.675405 | 2_4:+9214650 | Msa0313760:CDS |
| GAAACAAATTATATTCATGG+TGG | 0.683671 | 2_4:-9214388 | None:intergenic |
| ACGTTGCTAATACCAAACAA+TGG | 0.689270 | 2_4:+9214483 | Msa0313760:CDS |
| GATGCTCAAAATTTGGTGTG+GGG | 0.729523 | 2_4:+9214683 | Msa0313760:CDS |
| TTGTTTAGCATGGAACAACG+AGG | 0.763244 | 2_4:-9214062 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATTTGTTTCTCTTTAAA+AGG | + | chr2_4:9214399-9214418 | Msa0313760:CDS | 15.0% |
| !!! | ATAATTTGTTTCTCTTTAAA+AGG | + | chr2_4:9214399-9214418 | Msa0313760:CDS | 15.0% |
| !! | AGAGAAACAAATTATATTCA+TGG | - | chr2_4:9214394-9214413 | None:intergenic | 20.0% |
| !! | AGAGAAACAAATTATATTCA+TGG | - | chr2_4:9214394-9214413 | None:intergenic | 20.0% |
| !!! | TTTGCTTTTCTTTTTGCATA+TGG | - | chr2_4:9213865-9213884 | None:intergenic | 25.0% |
| ! | ATATGCAAAAAGAAAAGCAA+AGG | + | chr2_4:9213864-9213883 | Msa0313760:CDS | 25.0% |
| !!! | TGTATTTTTTCTCTAGAGAA+GGG | - | chr2_4:9214200-9214219 | None:intergenic | 25.0% |
| !!! | ATGTATTTTTTCTCTAGAGA+AGG | - | chr2_4:9214201-9214220 | None:intergenic | 25.0% |
| !! | CAGTTCTAATTTATATTGAG+TGG | - | chr2_4:9214321-9214340 | None:intergenic | 25.0% |
| ! | TATATTCATGGTGGTAAAAT+AGG | - | chr2_4:9214382-9214401 | None:intergenic | 25.0% |
| ! | GAAACAAATTATATTCATGG+TGG | - | chr2_4:9214391-9214410 | None:intergenic | 25.0% |
| ! | TATAACTACAAGTTTATTGC+TGG | - | chr2_4:9214459-9214478 | None:intergenic | 25.0% |
| !! | AGTATTGAAATGTAGATTGA+GGG | + | chr2_4:9214553-9214572 | Msa0313760:CDS | 25.0% |
| ! | ATTGTCAAAGAAAGCAAAAA+AGG | + | chr2_4:9214580-9214599 | Msa0313760:CDS | 25.0% |
| ! | TTGTCAAAGAAAGCAAAAAA+GGG | + | chr2_4:9214581-9214600 | Msa0313760:CDS | 25.0% |
| !!! | TTTGCTTTTCTTTTTGCATA+TGG | - | chr2_4:9213865-9213884 | None:intergenic | 25.0% |
| ! | ATATGCAAAAAGAAAAGCAA+AGG | + | chr2_4:9213864-9213883 | Msa0313760:CDS | 25.0% |
| !!! | TGTATTTTTTCTCTAGAGAA+GGG | - | chr2_4:9214200-9214219 | None:intergenic | 25.0% |
| !!! | ATGTATTTTTTCTCTAGAGA+AGG | - | chr2_4:9214201-9214220 | None:intergenic | 25.0% |
| !! | CAGTTCTAATTTATATTGAG+TGG | - | chr2_4:9214321-9214340 | None:intergenic | 25.0% |
| ! | TATATTCATGGTGGTAAAAT+AGG | - | chr2_4:9214382-9214401 | None:intergenic | 25.0% |
| ! | GAAACAAATTATATTCATGG+TGG | - | chr2_4:9214391-9214410 | None:intergenic | 25.0% |
| ! | TATAACTACAAGTTTATTGC+TGG | - | chr2_4:9214459-9214478 | None:intergenic | 25.0% |
| !! | AGTATTGAAATGTAGATTGA+GGG | + | chr2_4:9214553-9214572 | Msa0313760:CDS | 25.0% |
| ! | ATTGTCAAAGAAAGCAAAAA+AGG | + | chr2_4:9214580-9214599 | Msa0313760:CDS | 25.0% |
| ! | TTGTCAAAGAAAGCAAAAAA+GGG | + | chr2_4:9214581-9214600 | Msa0313760:CDS | 25.0% |
| ATATTTGACAGCAGATCATA+AGG | - | chr2_4:9213906-9213925 | None:intergenic | 30.0% | |
| TGCTGTCAAATATTGCAAAT+AGG | + | chr2_4:9213913-9213932 | Msa0313760:CDS | 30.0% | |
| ! | TTTATCATCCTTGTTTAGCA+TGG | - | chr2_4:9214075-9214094 | None:intergenic | 30.0% |
| !!! | GTATTTTTTCTCTAGAGAAG+GGG | - | chr2_4:9214199-9214218 | None:intergenic | 30.0% |
| ! | CAGTATTGAAATGTAGATTG+AGG | + | chr2_4:9214552-9214571 | Msa0313760:CDS | 30.0% |
| AATGTTGCAATAGATACTTG+TGG | + | chr2_4:9214650-9214669 | Msa0313760:CDS | 30.0% | |
| ATGTTGCAATAGATACTTGT+GGG | + | chr2_4:9214651-9214670 | Msa0313760:CDS | 30.0% | |
| ACTCTATGATGCTCAAAATT+TGG | + | chr2_4:9214676-9214695 | Msa0313760:CDS | 30.0% | |
| ! | TTTGATGATCATCTCCTTAA+AGG | + | chr2_4:9214731-9214750 | Msa0313760:CDS | 30.0% |
| ATATTTGACAGCAGATCATA+AGG | - | chr2_4:9213906-9213925 | None:intergenic | 30.0% | |
| TGCTGTCAAATATTGCAAAT+AGG | + | chr2_4:9213913-9213932 | Msa0313760:CDS | 30.0% | |
| ! | TTTATCATCCTTGTTTAGCA+TGG | - | chr2_4:9214075-9214094 | None:intergenic | 30.0% |
| !!! | GTATTTTTTCTCTAGAGAAG+GGG | - | chr2_4:9214199-9214218 | None:intergenic | 30.0% |
| ! | CAGTATTGAAATGTAGATTG+AGG | + | chr2_4:9214552-9214571 | Msa0313760:CDS | 30.0% |
| AATGTTGCAATAGATACTTG+TGG | + | chr2_4:9214650-9214669 | Msa0313760:CDS | 30.0% | |
| ATGTTGCAATAGATACTTGT+GGG | + | chr2_4:9214651-9214670 | Msa0313760:CDS | 30.0% | |
| ACTCTATGATGCTCAAAATT+TGG | + | chr2_4:9214676-9214695 | Msa0313760:CDS | 30.0% | |
| ! | TTTGATGATCATCTCCTTAA+AGG | + | chr2_4:9214731-9214750 | Msa0313760:CDS | 30.0% |
| AAAAGAAAAGCAAAGGAGAG+TGG | + | chr2_4:9213871-9213890 | Msa0313760:CDS | 35.0% | |
| GTCAAATATTGCAAATAGGC+TGG | + | chr2_4:9213917-9213936 | Msa0313760:CDS | 35.0% | |
| TTCATCATGTGTGCAAAGAT+TGG | + | chr2_4:9213961-9213980 | Msa0313760:CDS | 35.0% | |
| CTAAATTTAGCGTCAACCAA+AGG | - | chr2_4:9214002-9214021 | None:intergenic | 35.0% | |
| TTGGTTGACGCTAAATTTAG+TGG | + | chr2_4:9214002-9214021 | Msa0313760:CDS | 35.0% | |
| ! | AATTTAGTGGATGTGATCCA+TGG | + | chr2_4:9214015-9214034 | Msa0313760:CDS | 35.0% |
| TTTCTGTTGTATGGTGAAGA+AGG | + | chr2_4:9214038-9214057 | Msa0313760:CDS | 35.0% | |
| CACTATTGAAATTCCAGAAC+TGG | + | chr2_4:9214100-9214119 | Msa0313760:CDS | 35.0% | |
| GATTCTGTGATAGGACAATT+TGG | - | chr2_4:9214227-9214246 | None:intergenic | 35.0% | |
| AGGTGTGCATTCTTTATTGA+AGG | + | chr2_4:9214419-9214438 | Msa0313760:CDS | 35.0% | |
| GGTGTGCATTCTTTATTGAA+GGG | + | chr2_4:9214420-9214439 | Msa0313760:CDS | 35.0% | |
| GTGTGCATTCTTTATTGAAG+GGG | + | chr2_4:9214421-9214440 | Msa0313760:CDS | 35.0% | |
| ACGTTGCTAATACCAAACAA+TGG | + | chr2_4:9214483-9214502 | Msa0313760:CDS | 35.0% | |
| AAGGATCTGAAAGGAAAACT+AGG | + | chr2_4:9214617-9214636 | Msa0313760:CDS | 35.0% | |
| TGAAAGGAAAACTAGGACTT+GGG | + | chr2_4:9214624-9214643 | Msa0313760:CDS | 35.0% | |
| ! | ATGATGCTCAAAATTTGGTG+TGG | + | chr2_4:9214681-9214700 | Msa0313760:CDS | 35.0% |
| !! | TGATGCTCAAAATTTGGTGT+GGG | + | chr2_4:9214682-9214701 | Msa0313760:CDS | 35.0% |
| ATCATCTCCTTAAAGGAGTA+TGG | + | chr2_4:9214738-9214757 | Msa0313760:CDS | 35.0% | |
| AAAAGAAAAGCAAAGGAGAG+TGG | + | chr2_4:9213871-9213890 | Msa0313760:CDS | 35.0% | |
| GTCAAATATTGCAAATAGGC+TGG | + | chr2_4:9213917-9213936 | Msa0313760:CDS | 35.0% | |
| TTCATCATGTGTGCAAAGAT+TGG | + | chr2_4:9213961-9213980 | Msa0313760:CDS | 35.0% | |
| CTAAATTTAGCGTCAACCAA+AGG | - | chr2_4:9214002-9214021 | None:intergenic | 35.0% | |
| TTGGTTGACGCTAAATTTAG+TGG | + | chr2_4:9214002-9214021 | Msa0313760:CDS | 35.0% | |
| ! | AATTTAGTGGATGTGATCCA+TGG | + | chr2_4:9214015-9214034 | Msa0313760:CDS | 35.0% |
| TTTCTGTTGTATGGTGAAGA+AGG | + | chr2_4:9214038-9214057 | Msa0313760:CDS | 35.0% | |
| CACTATTGAAATTCCAGAAC+TGG | + | chr2_4:9214100-9214119 | Msa0313760:CDS | 35.0% | |
| GATTCTGTGATAGGACAATT+TGG | - | chr2_4:9214227-9214246 | None:intergenic | 35.0% | |
| AGGTGTGCATTCTTTATTGA+AGG | + | chr2_4:9214419-9214438 | Msa0313760:CDS | 35.0% | |
| GGTGTGCATTCTTTATTGAA+GGG | + | chr2_4:9214420-9214439 | Msa0313760:CDS | 35.0% | |
| GTGTGCATTCTTTATTGAAG+GGG | + | chr2_4:9214421-9214440 | Msa0313760:CDS | 35.0% | |
| ACGTTGCTAATACCAAACAA+TGG | + | chr2_4:9214483-9214502 | Msa0313760:CDS | 35.0% | |
| AAGGATCTGAAAGGAAAACT+AGG | + | chr2_4:9214617-9214636 | Msa0313760:CDS | 35.0% | |
| TGAAAGGAAAACTAGGACTT+GGG | + | chr2_4:9214624-9214643 | Msa0313760:CDS | 35.0% | |
| ! | ATGATGCTCAAAATTTGGTG+TGG | + | chr2_4:9214681-9214700 | Msa0313760:CDS | 35.0% |
| !! | TGATGCTCAAAATTTGGTGT+GGG | + | chr2_4:9214682-9214701 | Msa0313760:CDS | 35.0% |
| ATCATCTCCTTAAAGGAGTA+TGG | + | chr2_4:9214738-9214757 | Msa0313760:CDS | 35.0% | |
| GATCCATGGTTTCTGTTGTA+TGG | + | chr2_4:9214029-9214048 | Msa0313760:CDS | 40.0% | |
| TCACCATACAACAGAAACCA+TGG | - | chr2_4:9214035-9214054 | None:intergenic | 40.0% | |
| TTGTTTAGCATGGAACAACG+AGG | - | chr2_4:9214065-9214084 | None:intergenic | 40.0% | |
| TCGTTGTTCCATGCTAAACA+AGG | + | chr2_4:9214064-9214083 | Msa0313760:CDS | 40.0% | |
| ATTGAAATTCCAGAACTGGC+TGG | + | chr2_4:9214104-9214123 | Msa0313760:CDS | 40.0% | |
| TTGAAATTCCAGAACTGGCT+GGG | + | chr2_4:9214105-9214124 | Msa0313760:CDS | 40.0% | |
| CAGTCATTCGATTCTGTGAT+AGG | - | chr2_4:9214236-9214255 | None:intergenic | 40.0% | |
| TTGAGTGGCGTGATTAATCA+CGG | - | chr2_4:9214306-9214325 | None:intergenic | 40.0% | |
| TTTGTCGGGGAGATAATTCA+TGG | + | chr2_4:9214348-9214367 | Msa0313760:CDS | 40.0% | |
| ATGGAGCGAGTGTAGTTTAT+TGG | + | chr2_4:9214502-9214521 | Msa0313760:CDS | 40.0% | |
| !! | AAAGGGAAAGCATCGATCAA+AGG | + | chr2_4:9214598-9214617 | Msa0313760:CDS | 40.0% |
| CATCGATCAAAGGATCTGAA+AGG | + | chr2_4:9214608-9214627 | Msa0313760:CDS | 40.0% | |
| CTGAAAGGAAAACTAGGACT+TGG | + | chr2_4:9214623-9214642 | Msa0313760:CDS | 40.0% | |
| GAAAGGAAAACTAGGACTTG+GGG | + | chr2_4:9214625-9214644 | Msa0313760:CDS | 40.0% | |
| !! | GATGCTCAAAATTTGGTGTG+GGG | + | chr2_4:9214683-9214702 | Msa0313760:CDS | 40.0% |
| GATCCATGGTTTCTGTTGTA+TGG | + | chr2_4:9214029-9214048 | Msa0313760:CDS | 40.0% | |
| TCACCATACAACAGAAACCA+TGG | - | chr2_4:9214035-9214054 | None:intergenic | 40.0% | |
| TTGTTTAGCATGGAACAACG+AGG | - | chr2_4:9214065-9214084 | None:intergenic | 40.0% | |
| TCGTTGTTCCATGCTAAACA+AGG | + | chr2_4:9214064-9214083 | Msa0313760:CDS | 40.0% | |
| ATTGAAATTCCAGAACTGGC+TGG | + | chr2_4:9214104-9214123 | Msa0313760:CDS | 40.0% | |
| TTGAAATTCCAGAACTGGCT+GGG | + | chr2_4:9214105-9214124 | Msa0313760:CDS | 40.0% | |
| CAGTCATTCGATTCTGTGAT+AGG | - | chr2_4:9214236-9214255 | None:intergenic | 40.0% | |
| TTGAGTGGCGTGATTAATCA+CGG | - | chr2_4:9214306-9214325 | None:intergenic | 40.0% | |
| TTTGTCGGGGAGATAATTCA+TGG | + | chr2_4:9214348-9214367 | Msa0313760:CDS | 40.0% | |
| ATGGAGCGAGTGTAGTTTAT+TGG | + | chr2_4:9214502-9214521 | Msa0313760:CDS | 40.0% | |
| !! | AAAGGGAAAGCATCGATCAA+AGG | + | chr2_4:9214598-9214617 | Msa0313760:CDS | 40.0% |
| CATCGATCAAAGGATCTGAA+AGG | + | chr2_4:9214608-9214627 | Msa0313760:CDS | 40.0% | |
| CTGAAAGGAAAACTAGGACT+TGG | + | chr2_4:9214623-9214642 | Msa0313760:CDS | 40.0% | |
| GAAAGGAAAACTAGGACTTG+GGG | + | chr2_4:9214625-9214644 | Msa0313760:CDS | 40.0% | |
| !! | GATGCTCAAAATTTGGTGTG+GGG | + | chr2_4:9214683-9214702 | Msa0313760:CDS | 40.0% |
| ACAATGCAATCCTGAGACGT+GGG | - | chr2_4:9214281-9214300 | None:intergenic | 45.0% | |
| AACAATGCAATCCTGAGACG+TGG | - | chr2_4:9214282-9214301 | None:intergenic | 45.0% | |
| TAGAACTGCACACGCTTTGT+CGG | + | chr2_4:9214333-9214352 | Msa0313760:CDS | 45.0% | |
| ACTACACTCGCTCCATTGTT+TGG | - | chr2_4:9214498-9214517 | None:intergenic | 45.0% | |
| TGGTGTGGGGTGATTACATT+TGG | + | chr2_4:9214696-9214715 | Msa0313760:CDS | 45.0% | |
| GCACGATCCATACTCCTTTA+AGG | - | chr2_4:9214748-9214767 | None:intergenic | 45.0% | |
| ACAATGCAATCCTGAGACGT+GGG | - | chr2_4:9214281-9214300 | None:intergenic | 45.0% | |
| AACAATGCAATCCTGAGACG+TGG | - | chr2_4:9214282-9214301 | None:intergenic | 45.0% | |
| TAGAACTGCACACGCTTTGT+CGG | + | chr2_4:9214333-9214352 | Msa0313760:CDS | 45.0% | |
| ACTACACTCGCTCCATTGTT+TGG | - | chr2_4:9214498-9214517 | None:intergenic | 45.0% | |
| TGGTGTGGGGTGATTACATT+TGG | + | chr2_4:9214696-9214715 | Msa0313760:CDS | 45.0% | |
| GCACGATCCATACTCCTTTA+AGG | - | chr2_4:9214748-9214767 | None:intergenic | 45.0% | |
| ACTTGCCTTGCATCCTACGA+CGG | + | chr2_4:9214131-9214150 | Msa0313760:CDS | 50.0% | |
| AGAACTGCACACGCTTTGTC+GGG | + | chr2_4:9214334-9214353 | Msa0313760:CDS | 50.0% | |
| ACTTGCCTTGCATCCTACGA+CGG | + | chr2_4:9214131-9214150 | Msa0313760:CDS | 50.0% | |
| AGAACTGCACACGCTTTGTC+GGG | + | chr2_4:9214334-9214353 | Msa0313760:CDS | 50.0% | |
| ! | CAAGTTGACCCAGCCAGTTC+TGG | - | chr2_4:9214116-9214135 | None:intergenic | 55.0% |
| TTCATCTGCTCCCACGTCTC+AGG | + | chr2_4:9214268-9214287 | Msa0313760:CDS | 55.0% | |
| GAACTGCACACGCTTTGTCG+GGG | + | chr2_4:9214335-9214354 | Msa0313760:CDS | 55.0% | |
| ! | CAAGTTGACCCAGCCAGTTC+TGG | - | chr2_4:9214116-9214135 | None:intergenic | 55.0% |
| TTCATCTGCTCCCACGTCTC+AGG | + | chr2_4:9214268-9214287 | Msa0313760:CDS | 55.0% | |
| GAACTGCACACGCTTTGTCG+GGG | + | chr2_4:9214335-9214354 | Msa0313760:CDS | 55.0% | |
| GCGCTCTGCGACTGAACCTT+TGG | + | chr2_4:9213983-9214002 | Msa0313760:CDS | 60.0% | |
| GCCTTGCATCCTACGACGGA+TGG | + | chr2_4:9214135-9214154 | Msa0313760:CDS | 60.0% | |
| GCCATCCGTCGTAGGATGCA+AGG | - | chr2_4:9214139-9214158 | None:intergenic | 60.0% | |
| GAGAAGCAGCCATCCGTCGT+AGG | - | chr2_4:9214147-9214166 | None:intergenic | 60.0% | |
| GCGCTCTGCGACTGAACCTT+TGG | + | chr2_4:9213983-9214002 | Msa0313760:CDS | 60.0% | |
| GCCTTGCATCCTACGACGGA+TGG | + | chr2_4:9214135-9214154 | Msa0313760:CDS | 60.0% | |
| GCCATCCGTCGTAGGATGCA+AGG | - | chr2_4:9214139-9214158 | None:intergenic | 60.0% | |
| GAGAAGCAGCCATCCGTCGT+AGG | - | chr2_4:9214147-9214166 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 9213843 | 9214787 | 9213843 | ID=Msa0313760;Name=Msa0313760 |
| chr2_4 | mRNA | 9213843 | 9214787 | 9213843 | ID=Msa0313760-mRNA-1;Parent=Msa0313760;Name=Msa0313760-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|314 |
| chr2_4 | exon | 9213843 | 9214787 | 9213843 | ID=Msa0313760-mRNA-1:exon:3559;Parent=Msa0313760-mRNA-1 |
| chr2_4 | CDS | 9213843 | 9214787 | 9213843 | ID=Msa0313760-mRNA-1:cds;Parent=Msa0313760-mRNA-1 |
| Gene Sequence |
| Protein sequence |
