AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0921330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0921330 MtrunA17_Chr6g0478021 97.273 110 3 0 1 110 1 110 4.53e-77 222
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0921330 AT5G51960.2 59.091 110 38 1 1 110 1 103 1.55e-41 132
Msa0921330 AT5G51960.1 59.091 110 38 1 1 110 1 103 1.55e-41 132

Find 40 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTTGTAGGACTTTGTTTC+AGG 0.194152 6_2:+58592072 Msa0921330:three_prime_UTR
GATTATACTTACATGCTTAA+TGG 0.209140 6_2:+58591271 Msa0921330:CDS
AAATATCTACTGAATGAAAA+TGG 0.280545 6_2:+58591916 Msa0921330:three_prime_UTR
AAGGCGGTCAAGAAACATAT+TGG 0.364646 6_2:+58591139 Msa0921330:CDS
GGCGATAGATGTTAACAGTT+TGG 0.372669 6_2:-58591108 None:intergenic
TCCAATCAAACAAAATCAAC+TGG 0.387618 6_2:-58591894 None:intergenic
GGTTTATCAATCATAATGAT+TGG 0.401642 6_2:+58591788 Msa0921330:exon
AAGTAAGGATCTTTCCTTTG+AGG 0.413087 6_2:+58588963 Msa0921330:five_prime_UTR
TTTGTACTTGTGCAAAGAAA+AGG 0.429721 6_2:+58591843 Msa0921330:three_prime_UTR
TCTTGACCGCCTTGAGAAGA+TGG 0.432707 6_2:-58591129 None:intergenic
AATAGCGGCAGAAAACAAAT+AGG 0.434385 6_2:+58588904 Msa0921330:five_prime_UTR
AATGTTGAATCATGTTCATA+AGG 0.448220 6_2:+58592133 Msa0921330:three_prime_UTR
TCTACTGAATGAAAATGGTT+TGG 0.454953 6_2:+58591921 Msa0921330:three_prime_UTR
AACTATTGCCACTGTGTTGT+AGG 0.455204 6_2:+58592059 Msa0921330:three_prime_UTR
CCTCAGAGTTCCACAAGAAT+CGG 0.468221 6_2:+58591200 Msa0921330:CDS
AGGCGGTCAAGAAACATATT+GGG 0.477390 6_2:+58591140 Msa0921330:CDS
CCCTTGCAGGAAGTAGGTTG+TGG 0.510387 6_2:+58591052 Msa0921330:intron
ATCGTGAGGAACAAGAAGTA+AGG 0.516190 6_2:+58588948 Msa0921330:five_prime_UTR
ATAGATGTTAACAGTTTGGA+AGG 0.532971 6_2:-58591104 None:intergenic
CATTATGATTGATAAACCTC+GGG 0.535278 6_2:-58591783 None:intergenic
ACAGTGGCAATAGTTCCTAC+TGG 0.535562 6_2:-58592051 None:intergenic
AATAAGAACGGGCACCTCAA+AGG 0.545731 6_2:-58588977 None:intergenic
GTAAGAAATTAGTCGCTACA+TGG 0.550523 6_2:-58592106 None:intergenic
GGAAAATCTGCTGCAAGTGT+TGG 0.560044 6_2:+58591750 Msa0921330:CDS
CAGACAGATTCCGATTCTTG+TGG 0.562091 6_2:-58591210 None:intergenic
TCATTATGATTGATAAACCT+CGG 0.563220 6_2:-58591784 None:intergenic
CATCTATCGCCATCTTCTCA+AGG 0.575149 6_2:+58591120 Msa0921330:CDS
AATGAAGTGAAAAGAACACT+TGG 0.586563 6_2:+58591729 Msa0921330:CDS
TCGGAATCTGTCTGATGGTG+TGG 0.587780 6_2:+58591219 Msa0921330:CDS
CCGATTCTTGTGGAACTCTG+AGG 0.596469 6_2:-58591200 None:intergenic
AACAAAGCAATACCTTGTGA+TGG 0.597418 6_2:-58591300 None:intergenic
AAGAATCGGAATCTGTCTGA+TGG 0.612539 6_2:+58591214 Msa0921330:CDS
TAATGGTGTGCACCATCACA+AGG 0.617495 6_2:+58591288 Msa0921330:CDS
TGCCATCCCTTGCAGGAAGT+AGG 0.636656 6_2:+58591046 Msa0921330:intron
GATAAACCTCGGGAAGCTGA+AGG 0.641065 6_2:-58591773 None:intergenic
TGTTGGCCTTCAGCTTCCCG+AGG 0.654165 6_2:+58591767 Msa0921330:CDS
AGTAAACAGAATCCTAATAG+CGG 0.674851 6_2:+58588889 None:intergenic
ACAGAGACAGTGAAATCGTG+AGG 0.690419 6_2:+58588934 Msa0921330:five_prime_UTR
CTATCGCCATCTTCTCAAGG+CGG 0.739647 6_2:+58591123 Msa0921330:CDS
AACAAAGTCCTACAACACAG+TGG 0.789806 6_2:-58592067 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAAAAAATAAGAAC+GGG - chr6_2:58588991-58589010 None:intergenic 10.0%
!! CTAAAAAAAAAAAATAAGAA+CGG - chr6_2:58588992-58589011 None:intergenic 10.0%
!!! TATTTTACTTTATTTTTGAA+TGG - chr6_2:58589420-58589439 None:intergenic 10.0%
!!! AGTTTTTTTTATTTTGTTGT+GGG + chr6_2:58589055-58589074 Msa0921330:intron 15.0%
!!! AAATTCTAAAAAATGTTTCT+TGG - chr6_2:58591176-58591195 None:intergenic 15.0%
!!! CAGTTTTTTTTATTTTGTTG+TGG + chr6_2:58589054-58589073 Msa0921330:intron 20.0%
!! ACCTAGTGAAAAATTATTAT+GGG + chr6_2:58589478-58589497 Msa0921330:intron 20.0%
!! ACATGATTAATAAAATGTTG+AGG + chr6_2:58589507-58589526 Msa0921330:intron 20.0%
!! CATGATTAATAAAATGTTGA+GGG + chr6_2:58589508-58589527 Msa0921330:intron 20.0%
!!! TGTAATTTTCTGCATTATTT+TGG + chr6_2:58589637-58589656 Msa0921330:intron 20.0%
!! TAGATACTTATAGATTTCAT+TGG + chr6_2:58589809-58589828 Msa0921330:intron 20.0%
!!! TATGACACTATTTTAGAATT+TGG + chr6_2:58590030-58590049 Msa0921330:intron 20.0%
!!! ATGACACTATTTTAGAATTT+GGG + chr6_2:58590031-58590050 Msa0921330:intron 20.0%
!!! CTAACTGATATTATTAGTTT+TGG + chr6_2:58590758-58590777 Msa0921330:intron 20.0%
!! AAACTAAGAACCTAAAAAAT+GGG - chr6_2:58590977-58590996 None:intergenic 20.0%
!! AAAACTAAGAACCTAAAAAA+TGG - chr6_2:58590978-58590997 None:intergenic 20.0%
!!! TTTGGAATAGAACAAAATTT+AGG - chr6_2:58591024-58591043 None:intergenic 20.0%
!! AAAATTAGCAAAACAAAATG+TGG - chr6_2:58591638-58591657 None:intergenic 20.0%
!! AAATATCTACTGAATGAAAA+TGG + chr6_2:58591916-58591935 Msa0921330:three_prime_UTR 20.0%
!! TGAAAACTTCAAATAAAAAG+AGG + chr6_2:58591961-58591980 Msa0921330:three_prime_UTR 20.0%
! GACCTAGTGAAAAATTATTA+TGG + chr6_2:58589477-58589496 Msa0921330:intron 25.0%
! TTAATAAAATGTTGAGGGAA+TGG + chr6_2:58589513-58589532 Msa0921330:intron 25.0%
!! AATTCTACCTCTATTCTTTT+CGG + chr6_2:58589710-58589729 Msa0921330:intron 25.0%
! AAAAATTCCGAAAAGAATAG+AGG - chr6_2:58589720-58589739 None:intergenic 25.0%
! AAAATACGTCTCTTAGAATT+TGG + chr6_2:58589869-58589888 Msa0921330:intron 25.0%
! AAATACGTCTCTTAGAATTT+GGG + chr6_2:58589870-58589889 Msa0921330:intron 25.0%
!!! ACTATTTTAGAATTTGGGTT+GGG + chr6_2:58590036-58590055 Msa0921330:intron 25.0%
!!! ATCTCTAAAATTGCTTTTGT+AGG - chr6_2:58590162-58590181 None:intergenic 25.0%
! ACATGTTGATGTTTATTGAT+GGG - chr6_2:58590416-58590435 None:intergenic 25.0%
! AATCACGAAACTTAAAATAG+AGG + chr6_2:58590534-58590553 Msa0921330:intron 25.0%
! TACTCACTTCAATTATGTTT+GGG - chr6_2:58590717-58590736 None:intergenic 25.0%
! TTACTCACTTCAATTATGTT+TGG - chr6_2:58590718-58590737 None:intergenic 25.0%
! AAAGAAAGATTCACATAGTA+AGG - chr6_2:58590810-58590829 None:intergenic 25.0%
! AGAAGAATTGTAAGGAAAAA+AGG - chr6_2:58590874-58590893 None:intergenic 25.0%
! AAATTGTATTTCTTCCATCT+TGG + chr6_2:58590932-58590951 Msa0921330:intron 25.0%
!!! TCTTAGTTTTTCTTTTTCTC+CGG + chr6_2:58590988-58591007 Msa0921330:intron 25.0%
! GATTATACTTACATGCTTAA+TGG + chr6_2:58591271-58591290 Msa0921330:CDS 25.0%
! AGTTTATCTCATTAATGTAC+AGG - chr6_2:58591368-58591387 None:intergenic 25.0%
! AGATTAATGACAATGATTCA+TGG - chr6_2:58591665-58591684 None:intergenic 25.0%
! TCATTATGATTGATAAACCT+CGG - chr6_2:58591787-58591806 None:intergenic 25.0%
! GGTTTATCAATCATAATGAT+TGG + chr6_2:58591788-58591807 Msa0921330:exon 25.0%
!! TGTGTTAATTTTTGCATGTA+TGG + chr6_2:58591812-58591831 Msa0921330:three_prime_UTR 25.0%
! AATGTTGAATCATGTTCATA+AGG + chr6_2:58592133-58592152 Msa0921330:three_prime_UTR 25.0%
!! CAACCTTCATAAGTTGTTTT+CGG - chr6_2:58589246-58589265 None:intergenic 30.0%
AAGCTGAAAACAACTTATGA+AGG + chr6_2:58589311-58589330 Msa0921330:intron 30.0%
! GCCCATAATAATTTTTCACT+AGG - chr6_2:58589482-58589501 None:intergenic 30.0%
!! GTATGAAATTTGGTACTGAT+TGG + chr6_2:58589537-58589556 Msa0921330:intron 30.0%
! AACTAAAGCATCTCATTTGA+GGG - chr6_2:58589576-58589595 None:intergenic 30.0%
! ATTGACTCAAAGTAAATGCA+TGG + chr6_2:58589666-58589685 Msa0921330:intron 30.0%
!!! CACTATTTTAGAATTTGGGT+TGG + chr6_2:58590035-58590054 Msa0921330:intron 30.0%
ATGAACACGTGTTTATAAGT+GGG - chr6_2:58590107-58590126 None:intergenic 30.0%
!! TAAAATTGCTTTTGTAGGAC+AGG - chr6_2:58590157-58590176 None:intergenic 30.0%
CACATGTTGATGTTTATTGA+TGG - chr6_2:58590417-58590436 None:intergenic 30.0%
AATAAACATCAACATGTGCT+TGG + chr6_2:58590419-58590438 Msa0921330:intron 30.0%
AGAAAGAAGTAGAAGATACA+AGG + chr6_2:58590473-58590492 Msa0921330:intron 30.0%
AGAAACCAGATATCTATATG+TGG + chr6_2:58590561-58590580 Msa0921330:intron 30.0%
TATGTAATGAGCTGATTGAA+TGG + chr6_2:58590654-58590673 Msa0921330:intron 30.0%
ACTCACTTCAATTATGTTTG+GGG - chr6_2:58590716-58590735 None:intergenic 30.0%
!!! GTTTTGGAATCCAATATAAG+AGG + chr6_2:58590774-58590793 Msa0921330:intron 30.0%
GAGATCAAAGAAGAATTGTA+AGG - chr6_2:58590882-58590901 None:intergenic 30.0%
AAATTTAGGAACCTTGTTAC+CGG - chr6_2:58591010-58591029 None:intergenic 30.0%
ATAGATGTTAACAGTTTGGA+AGG - chr6_2:58591107-58591126 None:intergenic 30.0%
AATACAAGTGACTGTTTGAA+GGG - chr6_2:58591339-58591358 None:intergenic 30.0%
TGTTGCGAAATAAAAGTAAG+AGG + chr6_2:58591444-58591463 Msa0921330:intron 30.0%
TAAGACAATCTTCAGAGTAT+TGG - chr6_2:58591540-58591559 None:intergenic 30.0%
ATCATTGTCATTAATCTTGC+AGG + chr6_2:58591668-58591687 Msa0921330:intron 30.0%
AATGAAGTGAAAAGAACACT+TGG + chr6_2:58591729-58591748 Msa0921330:CDS 30.0%
CATTATGATTGATAAACCTC+GGG - chr6_2:58591786-58591805 None:intergenic 30.0%
! TTTTCTTTGCACAAGTACAA+AGG - chr6_2:58591844-58591863 None:intergenic 30.0%
TTTGTACTTGTGCAAAGAAA+AGG + chr6_2:58591843-58591862 Msa0921330:three_prime_UTR 30.0%
! AAAAGGTTTTCGTGCTTTAT+TGG + chr6_2:58591860-58591879 Msa0921330:three_prime_UTR 30.0%
!! ACCAGTTGATTTTGTTTGAT+TGG + chr6_2:58591893-58591912 Msa0921330:three_prime_UTR 30.0%
TCCAATCAAACAAAATCAAC+TGG - chr6_2:58591897-58591916 None:intergenic 30.0%
TCTACTGAATGAAAATGGTT+TGG + chr6_2:58591921-58591940 Msa0921330:three_prime_UTR 30.0%
!!! GTTTTTTCTTTTTCTCCAGT+AGG + chr6_2:58592036-58592055 Msa0921330:three_prime_UTR 30.0%
! TCTTTTACAGATGTTTGCAA+TGG + chr6_2:58592188-58592207 Msa0921330:three_prime_UTR 30.0%
!! ATTTGTTTTCTGCCGCTATT+AGG - chr6_2:58588904-58588923 None:intergenic 35.0%
AATAGCGGCAGAAAACAAAT+AGG + chr6_2:58588904-58588923 Msa0921330:five_prime_UTR 35.0%
AAGTAAGGATCTTTCCTTTG+AGG + chr6_2:58588963-58588982 Msa0921330:five_prime_UTR 35.0%
TAAGCGTGCATCATGATAAA+TGG + chr6_2:58589123-58589142 Msa0921330:intron 35.0%
AAGCGTGCATCATGATAAAT+GGG + chr6_2:58589124-58589143 Msa0921330:intron 35.0%
AAGCCGAAAACAACTTATGA+AGG + chr6_2:58589240-58589259 Msa0921330:intron 35.0%
GTTATCTCAAACAGTCTAAC+AGG + chr6_2:58589275-58589294 Msa0921330:intron 35.0%
AACAGTCTAACGAGTGTTTA+TGG + chr6_2:58589364-58589383 Msa0921330:intron 35.0%
ATCAATCTAAACATGCGTCA+AGG + chr6_2:58589442-58589461 Msa0921330:intron 35.0%
AGGGAATGGTGTATGAAATT+TGG + chr6_2:58589527-58589546 Msa0921330:intron 35.0%
! CAACTAAAGCATCTCATTTG+AGG - chr6_2:58589577-58589596 None:intergenic 35.0%
TTTAGTTGCATGATCGTTGT+TGG + chr6_2:58589589-58589608 Msa0921330:intron 35.0%
TAAATGCATGGACTTGATGT+TGG + chr6_2:58589678-58589697 Msa0921330:intron 35.0%
TGGTGAACATTGTATGATGA+TGG + chr6_2:58589763-58589782 Msa0921330:intron 35.0%
ACATTTGTAGGGATTGAGTT+AGG - chr6_2:58589901-58589920 None:intergenic 35.0%
TAACTCAATCCCTACAAATG+TGG + chr6_2:58589900-58589919 Msa0921330:intron 35.0%
AACTCAATCCCTACAAATGT+GGG + chr6_2:58589901-58589920 Msa0921330:intron 35.0%
TTAAGAGTCCCACATTTGTA+GGG - chr6_2:58589912-58589931 None:intergenic 35.0%
TGTTATCAAGTTACCGCTAT+GGG - chr6_2:58589984-58590003 None:intergenic 35.0%
ATGTTATCAAGTTACCGCTA+TGG - chr6_2:58589985-58590004 None:intergenic 35.0%
TGAACACGTGTTTATAAGTG+GGG - chr6_2:58590106-58590125 None:intergenic 35.0%
CATGAACACGTGTTTATAAG+TGG - chr6_2:58590108-58590127 None:intergenic 35.0%
TGTCATATCTCATCCAATGT+AGG + chr6_2:58590126-58590145 Msa0921330:intron 35.0%
CCACTGTTCATTCAATTCTA+TGG - chr6_2:58590213-58590232 None:intergenic 35.0%
CCATAGAATTGAATGAACAG+TGG + chr6_2:58590210-58590229 Msa0921330:intron 35.0%
GATTAACTATTTGGGTGCAT+GGG - chr6_2:58590369-58590388 None:intergenic 35.0%
TGATTAACTATTTGGGTGCA+TGG - chr6_2:58590370-58590389 None:intergenic 35.0%
! CGGCTACTGATTAACTATTT+GGG - chr6_2:58590377-58590396 None:intergenic 35.0%
GAACTCCACATATAGATATC+TGG - chr6_2:58590569-58590588 None:intergenic 35.0%
AACAAAAAATCCTCCATCCA+CGG - chr6_2:58590600-58590619 None:intergenic 35.0%
ATCATCCACTCCTCTTATAT+TGG - chr6_2:58590787-58590806 None:intergenic 35.0%
GAACCTAAAAAATGGGAGAA+GGG - chr6_2:58590970-58590989 None:intergenic 35.0%
AGAACCTAAAAAATGGGAGA+AGG - chr6_2:58590971-58590990 None:intergenic 35.0%
! TTTCTTTTTCTCCGGTAACA+AGG + chr6_2:58590996-58591015 Msa0921330:intron 35.0%
!!! GAGCAAGCTTTATTTTCTGT+TGG - chr6_2:58591249-58591268 None:intergenic 35.0%
AACAAAGCAATACCTTGTGA+TGG - chr6_2:58591303-58591322 None:intergenic 35.0%
CAATACAAGTGACTGTTTGA+AGG - chr6_2:58591340-58591359 None:intergenic 35.0%
! AAAAGTAAGAGGTGTAGAAG+TGG + chr6_2:58591455-58591474 Msa0921330:intron 35.0%
! CTCTGAAGATTGTCTTACAA+TGG + chr6_2:58591543-58591562 Msa0921330:intron 35.0%
CTGTAGCGTAAATCATTACT+AGG + chr6_2:58591605-58591624 Msa0921330:intron 35.0%
GTAAGAAATTAGTCGCTACA+TGG - chr6_2:58592109-58592128 None:intergenic 35.0%
ATCGTGAGGAACAAGAAGTA+AGG + chr6_2:58588948-58588967 Msa0921330:five_prime_UTR 40.0%
! AACATGCGTCAAGGATTTTG+TGG + chr6_2:58589451-58589470 Msa0921330:intron 40.0%
! CTTTTCGGAATTTTTCGCGA+AGG + chr6_2:58589725-58589744 Msa0921330:intron 40.0%
CATACAATGTTCACCACTAG+AGG - chr6_2:58589759-58589778 None:intergenic 40.0%
! CGTCTCTTAGAATTTGGGTT+TGG + chr6_2:58589875-58589894 Msa0921330:intron 40.0%
GTTAAGAGTCCCACATTTGT+AGG - chr6_2:58589913-58589932 None:intergenic 40.0%
! AGCGGTAACTTGATAACATG+TGG + chr6_2:58589986-58590005 Msa0921330:intron 40.0%
! CTTGATAACATGTGGCTCAA+CGG + chr6_2:58589994-58590013 Msa0921330:intron 40.0%
!! GGTTTTGTAAGGGTTGAGTT+CGG - chr6_2:58590062-58590081 None:intergenic 40.0%
GAACTCAACCCTTACAAAAC+CGG + chr6_2:58590061-58590080 Msa0921330:intron 40.0%
AACTCAACCCTTACAAAACC+GGG + chr6_2:58590062-58590081 Msa0921330:intron 40.0%
!! CATACAACCCGGTTTTGTAA+GGG - chr6_2:58590072-58590091 None:intergenic 40.0%
CTTACAAAACCGGGTTGTAT+GGG + chr6_2:58590071-58590090 Msa0921330:intron 40.0%
GAACACGTGTTTATAAGTGG+GGG - chr6_2:58590105-58590124 None:intergenic 40.0%
GAGATCTTTCCTGTCCTTAA+AGG + chr6_2:58590177-58590196 Msa0921330:intron 40.0%
! ATGGACTGATCAAGCCTTTA+AGG - chr6_2:58590194-58590213 None:intergenic 40.0%
! GCGGCTACTGATTAACTATT+TGG - chr6_2:58590378-58590397 None:intergenic 40.0%
AATAGTTAATCAGTAGCCGC+AGG + chr6_2:58590378-58590397 Msa0921330:intron 40.0%
GAAGTAGAAGATACAAGGAG+TGG + chr6_2:58590478-58590497 Msa0921330:intron 40.0%
ACAAAAAATCCTCCATCCAC+GGG - chr6_2:58590599-58590618 None:intergenic 40.0%
! CAATTATGTTTGGGGTAAGC+AGG - chr6_2:58590708-58590727 None:intergenic 40.0%
GGAATCCAATATAAGAGGAG+TGG + chr6_2:58590779-58590798 Msa0921330:intron 40.0%
!!! ACACCCTTCTCCCATTTTTT+AGG + chr6_2:58590964-58590983 Msa0921330:intron 40.0%
GGCGATAGATGTTAACAGTT+TGG - chr6_2:58591111-58591130 None:intergenic 40.0%
AAGGCGGTCAAGAAACATAT+TGG + chr6_2:58591139-58591158 Msa0921330:CDS 40.0%
AGGCGGTCAAGAAACATATT+GGG + chr6_2:58591140-58591159 Msa0921330:CDS 40.0%
AAGAATCGGAATCTGTCTGA+TGG + chr6_2:58591214-58591233 Msa0921330:CDS 40.0%
!! CAATCTTCAGAGTATTGGCT+TGG - chr6_2:58591535-58591554 None:intergenic 40.0%
AACTATTGCCACTGTGTTGT+AGG + chr6_2:58592059-58592078 Msa0921330:three_prime_UTR 40.0%
AACAAAGTCCTACAACACAG+TGG - chr6_2:58592070-58592089 None:intergenic 40.0%
! GTGTTGTAGGACTTTGTTTC+AGG + chr6_2:58592072-58592091 Msa0921330:three_prime_UTR 40.0%
ACAGAGACAGTGAAATCGTG+AGG + chr6_2:58588934-58588953 Msa0921330:five_prime_UTR 45.0%
AATAAGAACGGGCACCTCAA+AGG - chr6_2:58588980-58588999 None:intergenic 45.0%
TGGCTCAACGGATCTTGAAT+GGG + chr6_2:58590006-58590025 Msa0921330:intron 45.0%
!! CCATACAACCCGGTTTTGTA+AGG - chr6_2:58590073-58590092 None:intergenic 45.0%
CCTTACAAAACCGGGTTGTA+TGG + chr6_2:58590070-58590089 Msa0921330:intron 45.0%
GTTATCCTACCCATACAACC+CGG - chr6_2:58590083-58590102 None:intergenic 45.0%
AGGACAGGAAAGTCCTACAT+TGG - chr6_2:58590142-58590161 None:intergenic 45.0%
! CTGATCAAGCCTTTAAGGAC+AGG - chr6_2:58590189-58590208 None:intergenic 45.0%
CTTGGATAGCAGAGCTAGTT+TGG + chr6_2:58590437-58590456 Msa0921330:intron 45.0%
CATCTATCGCCATCTTCTCA+AGG + chr6_2:58591120-58591139 Msa0921330:CDS 45.0%
CCTCAGAGTTCCACAAGAAT+CGG + chr6_2:58591200-58591219 Msa0921330:CDS 45.0%
CAGACAGATTCCGATTCTTG+TGG - chr6_2:58591213-58591232 None:intergenic 45.0%
TAATGGTGTGCACCATCACA+AGG + chr6_2:58591288-58591307 Msa0921330:CDS 45.0%
GCAATTCAGCATAGCCAAGT+TGG + chr6_2:58591507-58591526 Msa0921330:intron 45.0%
!! GTATTGGCTTGGATCCAACT+TGG - chr6_2:58591524-58591543 None:intergenic 45.0%
GGAAAATCTGCTGCAAGTGT+TGG + chr6_2:58591750-58591769 Msa0921330:CDS 45.0%
ACAGTGGCAATAGTTCCTAC+TGG - chr6_2:58592054-58592073 None:intergenic 45.0%
GAAGGTTTGAGTGCCTCTAG+TGG + chr6_2:58589743-58589762 Msa0921330:intron 50.0%
TCTAGTCCTGGGCATGAAGA+GGG - chr6_2:58589939-58589958 None:intergenic 50.0%
! CGCTCTAGATGTCTAGTCCT+GGG - chr6_2:58589950-58589969 None:intergenic 50.0%
! ACGCTCTAGATGTCTAGTCC+TGG - chr6_2:58589951-58589970 None:intergenic 50.0%
! CTAGACATCTAGAGCGTGAC+CGG + chr6_2:58589954-58589973 Msa0921330:intron 50.0%
! TAGACATCTAGAGCGTGACC+GGG + chr6_2:58589955-58589974 Msa0921330:intron 50.0%
GTGGCTCAACGGATCTTGAA+TGG + chr6_2:58590005-58590024 Msa0921330:intron 50.0%
CAAAACCGGGTTGTATGGGT+AGG + chr6_2:58590075-58590094 Msa0921330:intron 50.0%
AACTATTTGGGTGCATGGGC+AGG - chr6_2:58590365-58590384 None:intergenic 50.0%
AGATACAAGGAGTGGTGCTC+TGG + chr6_2:58590486-58590505 Msa0921330:intron 50.0%
AAAATCCTCCATCCACGGGA+TGG - chr6_2:58590595-58590614 None:intergenic 50.0%
GGTGTTCTCTCTCACCAAGA+TGG - chr6_2:58590949-58590968 None:intergenic 50.0%
CCTGCAAGGGATGGCATATT+TGG - chr6_2:58591042-58591061 None:intergenic 50.0%
CCAAATATGCCATCCCTTGC+AGG + chr6_2:58591039-58591058 Msa0921330:intron 50.0%
AACCTACTTCCTGCAAGGGA+TGG - chr6_2:58591051-58591070 None:intergenic 50.0%
CCACAACCTACTTCCTGCAA+GGG - chr6_2:58591055-58591074 None:intergenic 50.0%
TCCACAACCTACTTCCTGCA+AGG - chr6_2:58591056-58591075 None:intergenic 50.0%
CTATCGCCATCTTCTCAAGG+CGG + chr6_2:58591123-58591142 Msa0921330:CDS 50.0%
TCTTGACCGCCTTGAGAAGA+TGG - chr6_2:58591132-58591151 None:intergenic 50.0%
CCGATTCTTGTGGAACTCTG+AGG - chr6_2:58591203-58591222 None:intergenic 50.0%
! TCGGAATCTGTCTGATGGTG+TGG + chr6_2:58591219-58591238 Msa0921330:CDS 50.0%
GATAAACCTCGGGAAGCTGA+AGG - chr6_2:58591776-58591795 None:intergenic 50.0%
AACACACCCTCTTCATGCCC+AGG + chr6_2:58589930-58589949 Msa0921330:intron 55.0%
GTCTAGTCCTGGGCATGAAG+AGG - chr6_2:58589940-58589959 None:intergenic 55.0%
AGTTACCGCTATGGGTGACC+CGG - chr6_2:58589976-58589995 None:intergenic 55.0%
!! TGGGAGCATCAAGAAGCCTG+CGG - chr6_2:58590397-58590416 None:intergenic 55.0%
! AGTTCTGACCATCCCGTGGA+TGG + chr6_2:58590584-58590603 Msa0921330:intron 55.0%
TGCCATCCCTTGCAGGAAGT+AGG + chr6_2:58591046-58591065 Msa0921330:intron 55.0%
CCCTTGCAGGAAGTAGGTTG+TGG + chr6_2:58591052-58591071 Msa0921330:intron 55.0%
! GTGGAGTTCTGACCATCCCG+TGG + chr6_2:58590580-58590599 Msa0921330:intron 60.0%
TCTGACCATCCCGTGGATGG+AGG + chr6_2:58590587-58590606 Msa0921330:intron 60.0%
!! TGTTGGCCTTCAGCTTCCCG+AGG + chr6_2:58591767-58591786 Msa0921330:CDS 60.0%
CGTGACCGGGTCACCCATAG+CGG + chr6_2:58589968-58589987 Msa0921330:intron 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 58588895 58592226 58588895 ID=Msa0921330;Name=Msa0921330
chr6_2 mRNA 58588895 58592226 58588895 ID=Msa0921330-mRNA-1;Parent=Msa0921330;Name=Msa0921330-mRNA-1;_AED=0.02;_eAED=0.02;_QI=120|1|1|1|0.5|0.33|3|423|110
chr6_2 exon 58588895 58588984 58588895 ID=Msa0921330-mRNA-1:exon:8717;Parent=Msa0921330-mRNA-1
chr6_2 exon 58591061 58591309 58591061 ID=Msa0921330-mRNA-1:exon:8718;Parent=Msa0921330-mRNA-1
chr6_2 exon 58591690 58592226 58591690 ID=Msa0921330-mRNA-1:exon:8719;Parent=Msa0921330-mRNA-1
chr6_2 five_prime_UTR 58588895 58588984 58588895 ID=Msa0921330-mRNA-1:five_prime_utr;Parent=Msa0921330-mRNA-1
chr6_2 five_prime_UTR 58591061 58591090 58591061 ID=Msa0921330-mRNA-1:five_prime_utr;Parent=Msa0921330-mRNA-1
chr6_2 CDS 58591091 58591309 58591091 ID=Msa0921330-mRNA-1:cds;Parent=Msa0921330-mRNA-1
chr6_2 CDS 58591690 58591803 58591690 ID=Msa0921330-mRNA-1:cds;Parent=Msa0921330-mRNA-1
chr6_2 three_prime_UTR 58591804 58592226 58591804 ID=Msa0921330-mRNA-1:three_prime_utr;Parent=Msa0921330-mRNA-1
Gene Sequence

>Msa0921330

ATGAGAACTGAAGCCTTCCAAACTGTTAACATCTATCGCCATCTTCTCAAGGCGGTCAAGAAACATATTGGGAAAGATGAGACCAAGAAACATTTTTTAGAATTTGTAACCTCAGAGTTCCACAAGAATCGGAATCTGTCTGATGGTGTGGCTGTCCAACAGAAAATAAAGCTTGCTCGTGATTATACTTACATGCTTAATGGTGTGCACCATCACAAGGATTTGCTTTTCTCATATAACATTGCAATTGATAGATCAAATGAAGTGAAAAGAACACTTGGAAAATCTGCTGCAAGTGTTGGCCTTCAGCTTCCCGAGGTTTATCAATCATAA
Protein sequence

>Msa0921330

MRTEAFQTVNIYRHLLKAVKKHIGKDETKKHFLEFVTSEFHKNRNLSDGVAVQQKIKLARDYTYMLNGVHHHKDLLFSYNIAIDRSNEVKRTLGKSAASVGLQLPEVYQS*