AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0172450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0172450 MtrunA17_Chr1g0190621 100.000 126 0 0 1 126 1 126 5.49e-88 251
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0172450 AT1G08780.1 71.318 129 34 1 1 126 1 129 1.70e-62 187

Find 58 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCAATGTTTCTAACTTTC+CGG 0.166301 1_4:+57507993 Msa0172450:three_prime_UTR
TAACTTATTTATAGATTTCT+AGG 0.180155 1_4:+57507870 Msa0172450:three_prime_UTR
TTTCTCTGCTTCTCAGGTTC+TGG 0.243160 1_4:+57504641 Msa0172450:five_prime_UTR
AAAATTCTGTATGGGAAGTT+TGG 0.251421 1_4:+57507819 Msa0172450:CDS
ACAATAAAGACTCCTTTCTT+TGG 0.267753 1_4:-57508055 None:intergenic
ACAGGAATCAAATGATAATT+TGG 0.284647 1_4:+57507605 Msa0172450:intron
AGAAGCGACAAGCCAGAAAT+TGG 0.310931 1_4:+57507743 Msa0172450:CDS
AACATTTGATACTTATGCTT+TGG 0.331012 1_4:+57508177 Msa0172450:three_prime_UTR
AGGTCGTTCGGTTTCAAATA+GGG 0.342219 1_4:+57507664 Msa0172450:CDS
GAGGTCGTTCGGTTTCAAAT+AGG 0.358066 1_4:+57507663 Msa0172450:CDS
GTTGATTCAAATTCGATGTA+TGG 0.367701 1_4:-57504702 None:intergenic
CTCCCAGGTAACTTCCGTTT+CGG 0.367751 1_4:-57504828 None:intergenic
CAATAAAGACTCCTTTCTTT+GGG 0.368723 1_4:-57508054 None:intergenic
CGAATTTGTTGATGTTCTGC+TGG 0.380247 1_4:-57504853 None:intergenic
AGATTGTGTCTCTTAATTGT+AGG 0.390561 1_4:+57507942 Msa0172450:three_prime_UTR
ACACTCTTTCTCTGCTTCTC+AGG 0.391951 1_4:+57504635 Msa0172450:five_prime_UTR
CGATCCAACCTAGTAATAAC+TGG 0.411872 1_4:+57508100 Msa0172450:three_prime_UTR
GATCCGAAACGGAAGTTACC+TGG 0.424382 1_4:+57504825 Msa0172450:CDS
CAGCAGAACATCAACAAATT+CGG 0.438286 1_4:+57504854 Msa0172450:CDS
GAACTGAAGAAAATTCTGTA+TGG 0.443165 1_4:+57507810 Msa0172450:CDS
TACAATTAAGAGACACAATC+TGG 0.461620 1_4:-57507940 None:intergenic
CTGCTCTCTACTTCATCCTT+AGG 0.477281 1_4:-57507705 None:intergenic
TCACAGATGAAGAGGTCGTT+CGG 0.479003 1_4:+57507652 Msa0172450:CDS
TTGAATCAACAAGATGCAAC+AGG 0.487875 1_4:+57504715 Msa0172450:exon
GATGTTCTGCTGGTCCTCCC+AGG 0.504132 1_4:-57504843 None:intergenic
AAGCAGAGAAAGAGTGTGAT+GGG 0.504996 1_4:-57504629 None:intergenic
GAGAAAGAGTGTGATGGGCC+TGG 0.506330 1_4:-57504624 None:intergenic
TTGACCAGTTATTACTAGGT+TGG 0.511692 1_4:-57508104 None:intergenic
CTTAACATATTTGAGCATGT+TGG 0.513113 1_4:+57508137 Msa0172450:three_prime_UTR
CAGTTCTTAAAATTTGAGCC+AGG 0.529405 1_4:+57504606 Msa0172450:five_prime_UTR
TTGTATTCTATCCCAAAGAA+AGG 0.533087 1_4:+57508043 Msa0172450:three_prime_UTR
GGTATTTGCTCATGTGCCTA+AGG 0.537164 1_4:+57507689 Msa0172450:CDS
AACTGAAGAAAATTCTGTAT+GGG 0.540625 1_4:+57507811 Msa0172450:CDS
TTGTGATGCGTAAAGGGAGG+AGG 0.548135 1_4:+57504803 Msa0172450:intron
GGTCGTTCGGTTTCAAATAG+GGG 0.554503 1_4:+57507665 Msa0172450:CDS
GGAATCAAATGATAATTTGG+AGG 0.556672 1_4:+57507608 Msa0172450:intron
AGCCGGAAAGTTAGAAACAT+TGG 0.556790 1_4:-57507995 None:intergenic
ACCGTTGACCAGTTATTACT+AGG 0.557040 1_4:-57508108 None:intergenic
GAAGCAGAGAAAGAGTGTGA+TGG 0.560791 1_4:-57504630 None:intergenic
TGGAGAATCTATCAATCTTG+AGG 0.571653 1_4:+57507839 Msa0172450:CDS
CTAAGGATGAAGTAGAGAGC+AGG 0.573213 1_4:+57507706 Msa0172450:CDS
GTTAATTCTCACAGATGAAG+AGG 0.578655 1_4:+57507644 Msa0172450:CDS
TGATGAGATCAAAATAGCTA+AGG 0.587658 1_4:+57504907 Msa0172450:CDS
AAATCAGTCAAAGTTCTATG+AGG 0.588949 1_4:-57507914 None:intergenic
ATCTCACAGAATGAACAAGC+CGG 0.592616 1_4:-57508012 None:intergenic
CCAGAAATTGGAAAAGCTTG+AGG 0.599943 1_4:+57507755 Msa0172450:CDS
ATCCGAAACGGAAGTTACCT+GGG 0.600013 1_4:+57504826 Msa0172450:CDS
CATTGGCAAACAGTGAAATG+AGG 0.613129 1_4:-57507978 None:intergenic
GGTTCTGGTCAAGCTTGCAG+TGG 0.615906 1_4:+57504656 Msa0172450:five_prime_UTR
CGTTCGGTTTCAAATAGGGG+AGG 0.636159 1_4:+57507668 Msa0172450:CDS
AGAATCTATCAATCTTGAGG+AGG 0.639907 1_4:+57507842 Msa0172450:CDS
AAAGGGAGGAGGATCCGAAA+CGG 0.642912 1_4:+57504814 Msa0172450:intron
ACCTAGTAATAACTGGTCAA+CGG 0.646427 1_4:+57508107 Msa0172450:three_prime_UTR
AGAACATCAACAAATTCGGA+AGG 0.648692 1_4:+57504858 Msa0172450:CDS
TTCAGACATCTGAGCAACCA+CGG 0.661375 1_4:-57507790 None:intergenic
GAAATTGGAAAAGCTTGAGG+AGG 0.664223 1_4:+57507758 Msa0172450:CDS
TGAGGAGGAGAAACAATCCG+TGG 0.717310 1_4:+57507773 Msa0172450:CDS
CGAAACGGAAGTTACCTGGG+AGG 0.771650 1_4:+57504829 Msa0172450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAAAATTAAAAAATAT+AGG + chr1_4:57506776-57506795 Msa0172450:intron 0.0%
!!! TTAATAACTTTTTTCAATTA+AGG - chr1_4:57505260-57505279 None:intergenic 10.0%
!! TTATTAAAATATGGTATAAA+CGG + chr1_4:57506895-57506914 Msa0172450:intron 10.0%
!! AATCTCAAAAATTCAATTTA+GGG - chr1_4:57504751-57504770 None:intergenic 15.0%
!!! ATTTTAAAGGAAAACAAAAA+TGG + chr1_4:57505466-57505485 Msa0172450:intron 15.0%
!! AAAAACAAAAATACAAAATC+TGG + chr1_4:57505602-57505621 Msa0172450:intron 15.0%
!!! TTTTCAATTTATTTCCATAA+AGG + chr1_4:57505984-57506003 Msa0172450:intron 15.0%
!! ATATATATAATTCTTGTGAA+TGG - chr1_4:57506326-57506345 None:intergenic 15.0%
!! AAAAAAAATATATCAGCAAT+AGG + chr1_4:57506464-57506483 Msa0172450:intron 15.0%
!! AAAAAAATATATCAGCAATA+GGG + chr1_4:57506465-57506484 Msa0172450:intron 15.0%
!!! GTAAATTATTTTGAGAAATA+TGG + chr1_4:57506581-57506600 Msa0172450:intron 15.0%
!!! ATACATGTCTTTTTTTTTTA+AGG - chr1_4:57506752-57506771 None:intergenic 15.0%
!! AATTATGGCTTATTAAAATA+TGG + chr1_4:57506886-57506905 Msa0172450:intron 15.0%
!! AAATAATATTTAGTTGTTTG+AGG + chr1_4:57507465-57507484 Msa0172450:intron 15.0%
!! AACATCAAAATATAAAACAA+TGG - chr1_4:57507562-57507581 None:intergenic 15.0%
!! TAACTTATTTATAGATTTCT+AGG + chr1_4:57507870-57507889 Msa0172450:three_prime_UTR 15.0%
!! CAATCTCAAAAATTCAATTT+AGG - chr1_4:57504752-57504771 None:intergenic 20.0%
!! AAATATAAGCTATAAGCTTA+AGG - chr1_4:57505127-57505146 None:intergenic 20.0%
!!! GAGTGTTTCATTTATTTTAA+AGG + chr1_4:57505453-57505472 Msa0172450:intron 20.0%
!!! TTTGTTTTTTCCATTCAAAT+AGG - chr1_4:57505591-57505610 None:intergenic 20.0%
!!! CTCAACATACATTTTTATTT+TGG + chr1_4:57505636-57505655 Msa0172450:intron 20.0%
!! CTCAAATAATGATAATTATC+TGG - chr1_4:57505889-57505908 None:intergenic 20.0%
!! TATGGAAATAAATTGAAAAC+AGG - chr1_4:57505983-57506002 None:intergenic 20.0%
!! TACAACCAAATTTATTAGAA+AGG - chr1_4:57506445-57506464 None:intergenic 20.0%
!! AACAACTATAAAAATTTACC+TGG + chr1_4:57506527-57506546 Msa0172450:intron 20.0%
!!! ATAAGCACTTGTTATATAAA+AGG - chr1_4:57507112-57507131 None:intergenic 20.0%
!!! TATATTTTGATGTTAATGCA+TGG + chr1_4:57507568-57507587 Msa0172450:intron 20.0%
!!! AAAGGTTTTGGAATAGAAAT+GGG + chr1_4:57504968-57504987 Msa0172450:intron 25.0%
! GGATATAAACTACTCATATA+GGG - chr1_4:57505012-57505031 None:intergenic 25.0%
! AGGATATAAACTACTCATAT+AGG - chr1_4:57505013-57505032 None:intergenic 25.0%
! AATTTATAAGCTCAGATAAG+TGG - chr1_4:57505080-57505099 None:intergenic 25.0%
!!! TTTTTCAATTAAGGCTACAA+TGG - chr1_4:57505251-57505270 None:intergenic 25.0%
! GTAGTGTTTGATAAAATTAG+CGG - chr1_4:57505297-57505316 None:intergenic 25.0%
! TAAAGGAAAACAAAAATGGT+TGG + chr1_4:57505470-57505489 Msa0172450:intron 25.0%
! TGATTCATATTGTATGATTC+TGG - chr1_4:57505558-57505577 None:intergenic 25.0%
! AAACTATAAGAACGATATAG+CGG - chr1_4:57505858-57505877 None:intergenic 25.0%
!! TATCGTTCTTATAGTTTTTG+AGG + chr1_4:57505861-57505880 Msa0172450:intron 25.0%
! TATAAGCTATTTATCCAAAC+GGG - chr1_4:57506011-57506030 None:intergenic 25.0%
! CTATAAGCTATTTATCCAAA+CGG - chr1_4:57506012-57506031 None:intergenic 25.0%
!! CTTACATAATTTGAAGTGTT+TGG + chr1_4:57506129-57506148 Msa0172450:intron 25.0%
! TCTCACCTTTCTAATAAATT+TGG + chr1_4:57506437-57506456 Msa0172450:intron 25.0%
! ACAATACAAATACAAATACC+AGG - chr1_4:57506548-57506567 None:intergenic 25.0%
! CGTGTGTTAATTAAAGAATA+AGG + chr1_4:57506694-57506713 Msa0172450:intron 25.0%
!! AAGAATAAGGTATTCTAGTT+AGG + chr1_4:57506707-57506726 Msa0172450:intron 25.0%
!!! ATATAGGCTTTGAAGAAAAA+AGG + chr1_4:57506792-57506811 Msa0172450:intron 25.0%
!!! TTTGTTTTCATAAGCTATCA+TGG - chr1_4:57507041-57507060 None:intergenic 25.0%
! TAAAGTTGTTTATCCAAACA+AGG + chr1_4:57507147-57507166 Msa0172450:intron 25.0%
! ACAGGAATCAAATGATAATT+TGG + chr1_4:57507605-57507624 Msa0172450:intron 25.0%
! AACTGAAGAAAATTCTGTAT+GGG + chr1_4:57507811-57507830 Msa0172450:CDS 25.0%
!!! GATTTCTAGGTTACTTTTTA+TGG + chr1_4:57507883-57507902 Msa0172450:three_prime_UTR 25.0%
! AACATTTGATACTTATGCTT+TGG + chr1_4:57508177-57508196 Msa0172450:three_prime_UTR 25.0%
!! TGGCTCAAATTTTAAGAACT+GGG - chr1_4:57504607-57504626 None:intergenic 30.0%
! GTTGATTCAAATTCGATGTA+TGG - chr1_4:57504705-57504724 None:intergenic 30.0%
ATTTGTTTGTGATGCGTAAA+GGG + chr1_4:57504797-57504816 Msa0172450:intron 30.0%
TGATGAGATCAAAATAGCTA+AGG + chr1_4:57504907-57504926 Msa0172450:CDS 30.0%
AGCTAAGGTAATCAATCTTT+AGG + chr1_4:57504922-57504941 Msa0172450:intron 30.0%
GCTAAGGTAATCAATCTTTA+GGG + chr1_4:57504923-57504942 Msa0172450:intron 30.0%
CATGTTCCAATCATATTCAA+AGG + chr1_4:57504950-57504969 Msa0172450:intron 30.0%
CCAAAACCTTTGAATATGAT+TGG - chr1_4:57504959-57504978 None:intergenic 30.0%
! CCAATCATATTCAAAGGTTT+TGG + chr1_4:57504956-57504975 Msa0172450:intron 30.0%
!!! CAAAGGTTTTGGAATAGAAA+TGG + chr1_4:57504967-57504986 Msa0172450:intron 30.0%
!! ATAGGGATTCTCTTTGATTT+TGG - chr1_4:57504995-57505014 None:intergenic 30.0%
TATAAGCTATAAGCTTAAGG+TGG - chr1_4:57505124-57505143 None:intergenic 30.0%
TGATGTATACATGTATGTGT+GGG - chr1_4:57505414-57505433 None:intergenic 30.0%
CATACAATATGAATCAGAGA+AGG + chr1_4:57505562-57505581 Msa0172450:intron 30.0%
!! TTATTAGAGAAAGCGTTCAT+CGG + chr1_4:57505804-57505823 Msa0172450:intron 30.0%
TCGGAAGAAAAAGAAATTAG+CGG + chr1_4:57505823-57505842 Msa0172450:intron 30.0%
CATTATTTGAGCTTAACTAC+TGG + chr1_4:57505898-57505917 Msa0172450:intron 30.0%
ATAAGCTATTTATCCAAACG+GGG - chr1_4:57506010-57506029 None:intergenic 30.0%
CTCGATAAGCTATTTATGTT+TGG + chr1_4:57506087-57506106 Msa0172450:intron 30.0%
!! TTTTTACAGCTTTTTAGTCG+TGG - chr1_4:57506221-57506240 None:intergenic 30.0%
ATGTGGTAAGCTCAAAATTA+TGG + chr1_4:57506871-57506890 Msa0172450:intron 30.0%
TCAAAATAAGCCAATCCAAA+CGG + chr1_4:57507004-57507023 Msa0172450:intron 30.0%
!! TTTATTTTCTGCTTGAGTCA+AGG - chr1_4:57507180-57507199 None:intergenic 30.0%
ATCCTCGAAAATCAATTATG+TGG + chr1_4:57507324-57507343 Msa0172450:intron 30.0%
!! AACCACATAATTGATTTTCG+AGG - chr1_4:57507329-57507348 None:intergenic 30.0%
GGAATCAAATGATAATTTGG+AGG + chr1_4:57507608-57507627 Msa0172450:intron 30.0%
GAACTGAAGAAAATTCTGTA+TGG + chr1_4:57507810-57507829 Msa0172450:CDS 30.0%
AAAATTCTGTATGGGAAGTT+TGG + chr1_4:57507819-57507838 Msa0172450:CDS 30.0%
AAATCAGTCAAAGTTCTATG+AGG - chr1_4:57507917-57507936 None:intergenic 30.0%
TACAATTAAGAGACACAATC+TGG - chr1_4:57507943-57507962 None:intergenic 30.0%
AGATTGTGTCTCTTAATTGT+AGG + chr1_4:57507942-57507961 Msa0172450:three_prime_UTR 30.0%
TTGTATTCTATCCCAAAGAA+AGG + chr1_4:57508043-57508062 Msa0172450:three_prime_UTR 30.0%
CAATAAAGACTCCTTTCTTT+GGG - chr1_4:57508057-57508076 None:intergenic 30.0%
ACAATAAAGACTCCTTTCTT+TGG - chr1_4:57508058-57508077 None:intergenic 30.0%
GTCTATCAGTAACAAAAATG+TGG + chr1_4:57508076-57508095 Msa0172450:three_prime_UTR 30.0%
CTTAACATATTTGAGCATGT+TGG + chr1_4:57508137-57508156 Msa0172450:three_prime_UTR 30.0%
!! CTGGCTCAAATTTTAAGAAC+TGG - chr1_4:57504608-57504627 None:intergenic 35.0%
CAGTTCTTAAAATTTGAGCC+AGG + chr1_4:57504606-57504625 Msa0172450:five_prime_UTR 35.0%
TTGAATCAACAAGATGCAAC+AGG + chr1_4:57504715-57504734 Msa0172450:exon 35.0%
CATTTGTTTGTGATGCGTAA+AGG + chr1_4:57504796-57504815 Msa0172450:intron 35.0%
CAGCAGAACATCAACAAATT+CGG + chr1_4:57504854-57504873 Msa0172450:CDS 35.0%
AGAACATCAACAAATTCGGA+AGG + chr1_4:57504858-57504877 Msa0172450:CDS 35.0%
! CAACAAAAAGCATGTAGCAA+AGG - chr1_4:57505033-57505052 None:intergenic 35.0%
TTGAGATAGTGCTATCTGTT+TGG + chr1_4:57505050-57505069 Msa0172450:intron 35.0%
AAATAGCGAATCGCTGATAA+CGG - chr1_4:57505345-57505364 None:intergenic 35.0%
GTGGGAATAAGAATTGTTTG+TGG - chr1_4:57505396-57505415 None:intergenic 35.0%
GTGATGTATACATGTATGTG+TGG - chr1_4:57505415-57505434 None:intergenic 35.0%
ACTCTGTCTTTGTTCATATG+AGG - chr1_4:57505437-57505456 None:intergenic 35.0%
!! TTCTGGTTCCTATTTCCTAT+CGG - chr1_4:57505541-57505560 None:intergenic 35.0%
GAGAAGGTTTCCTATTTGAA+TGG + chr1_4:57505578-57505597 Msa0172450:intron 35.0%
TTGATCAGTTCTATAGTGCT+AGG - chr1_4:57505684-57505703 None:intergenic 35.0%
AGGGGGTCATATATATATAC+TGG + chr1_4:57506829-57506848 Msa0172450:intron 35.0%
TTACCCAAACGCATTTGAAA+AGG + chr1_4:57506950-57506969 Msa0172450:intron 35.0%
! AAGCCTTTTCAAATGCGTTT+GGG - chr1_4:57506956-57506975 None:intergenic 35.0%
! TAAGCCTTTTCAAATGCGTT+TGG - chr1_4:57506957-57506976 None:intergenic 35.0%
CAAAATAAGCCAATCCAAAC+GGG + chr1_4:57507005-57507024 Msa0172450:intron 35.0%
AAAATAAGCCAATCCAAACG+GGG + chr1_4:57507006-57507025 Msa0172450:intron 35.0%
ATAAGCTATCATGGAGAGTT+TGG - chr1_4:57507032-57507051 None:intergenic 35.0%
TGTGATGTCTTTCAACTTCT+CGG + chr1_4:57507435-57507454 Msa0172450:intron 35.0%
TTGCAAAATGCTTAGAAGGA+GGG - chr1_4:57507531-57507550 None:intergenic 35.0%
ATTGCAAAATGCTTAGAAGG+AGG - chr1_4:57507532-57507551 None:intergenic 35.0%
GTCATTGCAAAATGCTTAGA+AGG - chr1_4:57507535-57507554 None:intergenic 35.0%
!! ATGGAGATTTTGTTTGTCAC+AGG + chr1_4:57507587-57507606 Msa0172450:intron 35.0%
GTTAATTCTCACAGATGAAG+AGG + chr1_4:57507644-57507663 Msa0172450:CDS 35.0%
TGGAGAATCTATCAATCTTG+AGG + chr1_4:57507839-57507858 Msa0172450:CDS 35.0%
AGAATCTATCAATCTTGAGG+AGG + chr1_4:57507842-57507861 Msa0172450:CDS 35.0%
! TGCCAATGTTTCTAACTTTC+CGG + chr1_4:57507993-57508012 Msa0172450:three_prime_UTR 35.0%
TTGACCAGTTATTACTAGGT+TGG - chr1_4:57508107-57508126 None:intergenic 35.0%
ACCTAGTAATAACTGGTCAA+CGG + chr1_4:57508107-57508126 Msa0172450:three_prime_UTR 35.0%
! AAGCAGAGAAAGAGTGTGAT+GGG - chr1_4:57504632-57504651 None:intergenic 40.0%
!! CGAATTTGTTGATGTTCTGC+TGG - chr1_4:57504856-57504875 None:intergenic 40.0%
AGAAACTGTACGTCTCTGTT+TGG - chr1_4:57505372-57505391 None:intergenic 40.0%
ATTTCCTATCGGGATGATTC+TGG - chr1_4:57505530-57505549 None:intergenic 40.0%
GAATCATCCCGATAGGAAAT+AGG + chr1_4:57505530-57505549 Msa0172450:intron 40.0%
! TCTGGTTCCTATTTCCTATC+GGG - chr1_4:57505540-57505559 None:intergenic 40.0%
TGCTAGGAGTTCAAAGTAAG+AGG - chr1_4:57505668-57505687 None:intergenic 40.0%
!! AAAAGGTAAGCGAGTATCGA+AGG + chr1_4:57506809-57506828 Msa0172450:intron 40.0%
!! AAAGGTAAGCGAGTATCGAA+GGG + chr1_4:57506810-57506829 Msa0172450:intron 40.0%
TAAGCTCCACATAAGCTATG+TGG + chr1_4:57506854-57506873 Msa0172450:intron 40.0%
AGCTTACCACATAGCTTATG+TGG - chr1_4:57506863-57506882 None:intergenic 40.0%
CTTTGCTAGGTTTGAGGAAA+AGG + chr1_4:57507260-57507279 Msa0172450:intron 40.0%
TTTGAGGAAAAGGATGAGAG+TGG + chr1_4:57507270-57507289 Msa0172450:intron 40.0%
ACACGACACACATGAGAAAA+GGG - chr1_4:57507362-57507381 None:intergenic 40.0%
TGTCTTTCAACTTCTCGGTT+TGG + chr1_4:57507440-57507459 Msa0172450:intron 40.0%
TGCAAAATGCTTAGAAGGAG+GGG - chr1_4:57507530-57507549 None:intergenic 40.0%
AGGTCGTTCGGTTTCAAATA+GGG + chr1_4:57507664-57507683 Msa0172450:CDS 40.0%
! CCTCAAGCTTTTCCAATTTC+TGG - chr1_4:57507758-57507777 None:intergenic 40.0%
CCAGAAATTGGAAAAGCTTG+AGG + chr1_4:57507755-57507774 Msa0172450:CDS 40.0%
GAAATTGGAAAAGCTTGAGG+AGG + chr1_4:57507758-57507777 Msa0172450:CDS 40.0%
CATTGGCAAACAGTGAAATG+AGG - chr1_4:57507981-57508000 None:intergenic 40.0%
AGCCGGAAAGTTAGAAACAT+TGG - chr1_4:57507998-57508017 None:intergenic 40.0%
ATCTCACAGAATGAACAAGC+CGG - chr1_4:57508015-57508034 None:intergenic 40.0%
CGATCCAACCTAGTAATAAC+TGG + chr1_4:57508100-57508119 Msa0172450:three_prime_UTR 40.0%
ACCGTTGACCAGTTATTACT+AGG - chr1_4:57508111-57508130 None:intergenic 40.0%
! GAAGCAGAGAAAGAGTGTGA+TGG - chr1_4:57504633-57504652 None:intergenic 45.0%
ACACTCTTTCTCTGCTTCTC+AGG + chr1_4:57504635-57504654 Msa0172450:five_prime_UTR 45.0%
TTTCTCTGCTTCTCAGGTTC+TGG + chr1_4:57504641-57504660 Msa0172450:five_prime_UTR 45.0%
TGTTTGTGATGCGTAAAGGG+AGG + chr1_4:57504800-57504819 Msa0172450:intron 45.0%
ATCCGAAACGGAAGTTACCT+GGG + chr1_4:57504826-57504845 Msa0172450:CDS 45.0%
! TATCGGGATGATTCTGGTTG+TGG - chr1_4:57505524-57505543 None:intergenic 45.0%
ACAACCAGAATCATCCCGAT+AGG + chr1_4:57505523-57505542 Msa0172450:intron 45.0%
TTCCCGCAAAAGAACATGCT+TGG + chr1_4:57505772-57505791 Msa0172450:intron 45.0%
TCCCGCAAAAGAACATGCTT+GGG + chr1_4:57505773-57505792 Msa0172450:intron 45.0%
TAAGCGCTTGAGAGACTGTT+TGG + chr1_4:57505923-57505942 Msa0172450:intron 45.0%
AAGCGCTTGAGAGACTGTTT+GGG + chr1_4:57505924-57505943 Msa0172450:intron 45.0%
ATTTCCATAAAGGCCCCGTT+TGG + chr1_4:57505994-57506013 Msa0172450:intron 45.0%
TTATCCAAACGGGGCCTTTA+TGG - chr1_4:57506001-57506020 None:intergenic 45.0%
!! AAGGTAAGCGAGTATCGAAG+GGG + chr1_4:57506811-57506830 Msa0172450:intron 45.0%
! TCAAGGAGATCTGCCTTGTT+TGG - chr1_4:57507163-57507182 None:intergenic 45.0%
CAAACCTAGCAAAGAACTCG+AGG - chr1_4:57507254-57507273 None:intergenic 45.0%
CGAGTTCTTTGCTAGGTTTG+AGG + chr1_4:57507254-57507273 Msa0172450:intron 45.0%
CACACGACACACATGAGAAA+AGG - chr1_4:57507363-57507382 None:intergenic 45.0%
TCACAGATGAAGAGGTCGTT+CGG + chr1_4:57507652-57507671 Msa0172450:CDS 45.0%
GAGGTCGTTCGGTTTCAAAT+AGG + chr1_4:57507663-57507682 Msa0172450:CDS 45.0%
GGTCGTTCGGTTTCAAATAG+GGG + chr1_4:57507665-57507684 Msa0172450:CDS 45.0%
GGTATTTGCTCATGTGCCTA+AGG + chr1_4:57507689-57507708 Msa0172450:CDS 45.0%
CTGCTCTCTACTTCATCCTT+AGG - chr1_4:57507708-57507727 None:intergenic 45.0%
CTAAGGATGAAGTAGAGAGC+AGG + chr1_4:57507706-57507725 Msa0172450:CDS 45.0%
AGAAGCGACAAGCCAGAAAT+TGG + chr1_4:57507743-57507762 Msa0172450:CDS 45.0%
TTCAGACATCTGAGCAACCA+CGG - chr1_4:57507793-57507812 None:intergenic 45.0%
TTGTGATGCGTAAAGGGAGG+AGG + chr1_4:57504803-57504822 Msa0172450:intron 50.0%
AAAGGGAGGAGGATCCGAAA+CGG + chr1_4:57504814-57504833 Msa0172450:intron 50.0%
GATCCGAAACGGAAGTTACC+TGG + chr1_4:57504825-57504844 Msa0172450:CDS 50.0%
CTCCCAGGTAACTTCCGTTT+CGG - chr1_4:57504831-57504850 None:intergenic 50.0%
!! GCGAATCGCTGATAACGGAT+AGG - chr1_4:57505340-57505359 None:intergenic 50.0%
! GGGATGATTCTGGTTGTGGT+GGG - chr1_4:57505520-57505539 None:intergenic 50.0%
! CCCCAAGCATGTTCTTTTGC+GGG - chr1_4:57505777-57505796 None:intergenic 50.0%
CCCGCAAAAGAACATGCTTG+GGG + chr1_4:57505774-57505793 Msa0172450:intron 50.0%
! CCCCCAAGCATGTTCTTTTG+CGG - chr1_4:57505778-57505797 None:intergenic 50.0%
CCGCAAAAGAACATGCTTGG+GGG + chr1_4:57505775-57505794 Msa0172450:intron 50.0%
AAGAACATGCTTGGGGGTGA+TGG + chr1_4:57505781-57505800 Msa0172450:intron 50.0%
!! AGGTAAGCGAGTATCGAAGG+GGG + chr1_4:57506812-57506831 Msa0172450:intron 50.0%
AAGAACTCGAGGTAGGCAAC+AGG - chr1_4:57507243-57507262 None:intergenic 50.0%
CCTAGCAAAGAACTCGAGGT+AGG - chr1_4:57507250-57507269 None:intergenic 50.0%
CCTACCTCGAGTTCTTTGCT+AGG + chr1_4:57507247-57507266 Msa0172450:intron 50.0%
CGTTCGGTTTCAAATAGGGG+AGG + chr1_4:57507668-57507687 Msa0172450:CDS 50.0%
TGAGGAGGAGAAACAATCCG+TGG + chr1_4:57507773-57507792 Msa0172450:CDS 50.0%
!! GAGAAAGAGTGTGATGGGCC+TGG - chr1_4:57504627-57504646 None:intergenic 55.0%
! GGTTCTGGTCAAGCTTGCAG+TGG + chr1_4:57504656-57504675 Msa0172450:five_prime_UTR 55.0%
CGAAACGGAAGTTACCTGGG+AGG + chr1_4:57504829-57504848 Msa0172450:CDS 55.0%
! CGGGATGATTCTGGTTGTGG+TGG - chr1_4:57505521-57505540 None:intergenic 55.0%
GAGTTTGGCCCCGTTTGGAT+TGG - chr1_4:57507017-57507036 None:intergenic 55.0%
ATGGAGAGTTTGGCCCCGTT+TGG - chr1_4:57507022-57507041 None:intergenic 55.0%
GTGTGTCGTGTGTGTCTGAG+AGG + chr1_4:57507371-57507390 Msa0172450:intron 55.0%
GATGTTCTGCTGGTCCTCCC+AGG - chr1_4:57504846-57504865 None:intergenic 60.0%
GCGGGAAGTCTAGCACATCG+AGG - chr1_4:57505759-57505778 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 57504594 57508227 57504594 ID=Msa0172450;Name=Msa0172450
chr1_4 mRNA 57504594 57508227 57504594 ID=Msa0172450-mRNA-1;Parent=Msa0172450;Name=Msa0172450-mRNA-1;_AED=0.01;_eAED=0.01;_QI=134|1|1|1|1|1|3|358|126
chr1_4 exon 57504594 57504736 57504594 ID=Msa0172450-mRNA-1:exon:17598;Parent=Msa0172450-mRNA-1
chr1_4 exon 57504818 57504928 57504818 ID=Msa0172450-mRNA-1:exon:17599;Parent=Msa0172450-mRNA-1
chr1_4 exon 57507609 57508227 57507609 ID=Msa0172450-mRNA-1:exon:17600;Parent=Msa0172450-mRNA-1
chr1_4 five_prime_UTR 57504594 57504727 57504594 ID=Msa0172450-mRNA-1:five_prime_utr;Parent=Msa0172450-mRNA-1
chr1_4 CDS 57504728 57504736 57504728 ID=Msa0172450-mRNA-1:cds;Parent=Msa0172450-mRNA-1
chr1_4 CDS 57504818 57504928 57504818 ID=Msa0172450-mRNA-1:cds;Parent=Msa0172450-mRNA-1
chr1_4 CDS 57507609 57507869 57507609 ID=Msa0172450-mRNA-1:cds;Parent=Msa0172450-mRNA-1
chr1_4 three_prime_UTR 57507870 57508227 57507870 ID=Msa0172450-mRNA-1:three_prime_utr;Parent=Msa0172450-mRNA-1
Gene Sequence

>Msa0172450

ATGCAACAGGGAGGAGGATCCGAAACGGAAGTTACCTGGGAGGACCAGCAGAACATCAACAAATTCGGAAGGTTGAATAATCGCTTACACGAGCTACATGATGAGATCAAAATAGCTAAGGAATCAAATGATAATTTGGAGGATGCAAGCAATGAGTTAATTCTCACAGATGAAGAGGTCGTTCGGTTTCAAATAGGGGAGGTATTTGCTCATGTGCCTAAGGATGAAGTAGAGAGCAGGATAGAACAGATGCAAGAAGCGACAAGCCAGAAATTGGAAAAGCTTGAGGAGGAGAAACAATCCGTGGTTGCTCAGATGTCTGAACTGAAGAAAATTCTGTATGGGAAGTTTGGAGAATCTATCAATCTTGAGGAGGATTAG
Protein sequence

>Msa0172450

MQQGGGSETEVTWEDQQNINKFGRLNNRLHELHDEIKIAKESNDNLEDASNELILTDEEVVRFQIGEVFAHVPKDEVESRIEQMQEATSQKLEKLEEEKQSVVAQMSELKKILYGKFGESINLEED*