AlfalfaGEDB | Msa0270160

M. sativa cultivar zhongmu-4 / Msa0270160

KEGG: K03233
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0003824 GO:0004364 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006412 GO:0006414 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0010467 GO:0016020 GO:0016740 GO:0016765 GO:0019538 GO:0030054 GO:0030312 GO:0034641 GO:0034645 GO:0042221 GO:0043043 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0050896 GO:0051186 GO:0055044 GO:0071704 GO:0071944 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576
PFAM: PF02798.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0270160	XP_003592901.1	98.565	418	6	0	1	418	1	418	0.0	854

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0270160	sp\|Q9FUM1\|EF1G_PRUAV	78.910	422	82	4	3	418	2	422	0.0	656

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001390	Msa0270160	0.812724	3.671298e-51	-8.615850e-47
Msa0001760	Msa0270160	0.818139	2.290215e-52	-8.615850e-47
Msa0016440	Msa0270160	0.803081	4.131935e-49	-8.615850e-47
Msa0021320	Msa0270160	0.805095	1.574805e-49	-8.615850e-47
Msa0029730	Msa0270160	0.802641	5.095703e-49	-8.615850e-47
Msa0040220	Msa0270160	0.830918	2.240388e-55	-8.615850e-47
Msa0042510	Msa0270160	0.862122	6.335801e-64	-8.615850e-47
Msa0071640	Msa0270160	0.858186	9.789294e-63	-8.615850e-47
Msa0071760	Msa0270160	0.822927	1.823194e-53	-8.615850e-47
Msa0079560	Msa0270160	0.876606	1.221676e-68	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0270160	MtrunA17_Chr2g0276951	98.565	418	6	0	1	418	1	418	0.0	854
Msa0270160	MtrunA17_Chr3g0102331	89.021	419	42	3	3	418	2	419	0.0	769
Msa0270160	MtrunA17_Chr0c12g0493651	83.055	419	67	3	3	418	2	419	0.0	678
Msa0270160	MtrunA17_Chr4g0077001	56.522	322	52	3	13	333	12	246	1.23e-114	335
Msa0270160	MtrunA17_Chr4g0077011	90.769	65	6	0	344	408	1	65	1.11e-38	135
Msa0270160	MtrunA17_Chr3g0109891	30.233	172	107	6	42	203	40	208	1.19e-13	69.7
Msa0270160	MtrunA17_Chr1g0157211	28.221	163	107	4	16	169	16	177	5.34e-12	65.1
Msa0270160	MtrunA17_Chr1g0195831	27.014	211	125	5	13	205	11	210	6.44e-12	64.7
Msa0270160	MtrunA17_Chr3g0095781	25.714	175	113	4	42	204	39	208	8.23e-12	64.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0270160	AT1G09640.1	76.555	418	91	4	3	418	2	414	0.0	641
Msa0270160	AT1G57720.1	74.341	417	101	5	3	418	2	413	0.0	627
Msa0270160	AT1G57720.2	74.341	417	101	5	3	418	2	413	0.0	627
Msa0270160	AT1G09640.2	69.608	204	60	2	3	205	2	204	7.31e-97	291
Msa0270160	AT1G09640.2	88.732	71	7	1	349	418	195	265	1.02e-35	132
Msa0270160	AT2G30870.1	30.075	133	84	3	38	162	35	166	7.82e-13	67.8
Msa0270160	AT2G30860.1	29.371	143	94	2	27	162	24	166	1.71e-12	67.0
Msa0270160	AT3G62760.1	28.141	199	128	6	16	203	14	208	2.44e-12	66.6
Msa0270160	AT5G17220.1	28.655	171	113	3	42	204	40	209	1.30e-11	64.3

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Find 134 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGCCTCTCCCTCAAA+TGG	0.468428	2_3:-1463176	None:intergenic
AAAATGTCGAAATGATGTTT+AGG	0.371746	2_3:+1463467	Msa0270160:three_prime_UTR
AAATATAGAATTTGTAACTC+TGG	0.457667	2_3:-1463377	None:intergenic
AACAGAATGCCCGACCAAGT+AGG	0.668821	2_3:-1462209	None:intergenic
AACCAGAGAGAGTATCCCTC+TGG	0.541067	2_3:-1462861	None:intergenic
AACCCCTTCACCTTAAATGG+AGG	0.653598	2_3:-1463014	None:intergenic
AACGAGAGAGAGCTGCGCGT+TGG	0.552923	2_3:+1460540	Msa0270160:five_prime_UTR
AAGAAGGTCAAGAGGATTCT+TGG	0.332287	2_3:-1462533	None:intergenic
AAGTTGTACCTGCCAAGACT+TGG	0.560946	2_3:+1461609	Msa0270160:CDS
AATCAATCCACTGGTCAATG+TGG	0.571450	2_3:-1461554	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTATCAACATGAACCCTAT+TGG	+	chr2_3:1460797-1460816	Msa0270160:CDS	30.0%
AATCAGAGAAAAAGTAAGTG+TGG	-	chr2_3:1461295-1461314	None:intergenic	30.0%
ATAGATTTAATCTTGGCAGT+TGG	-	chr2_3:1461413-1461432	None:intergenic	30.0%
TATAGAGGGTAAATTTGCAA+AGG	+	chr2_3:1461470-1461489	Msa0270160:intron	30.0%
GGGAAAATATTCAGTAAAAG+GGG	-	chr2_3:1462093-1462112	None:intergenic	30.0%
TGTTGTTATAATGTCAGCAA+GGG	-	chr2_3:1462236-1462255	None:intergenic	30.0%
ATGTTGTTATAATGTCAGCA+AGG	-	chr2_3:1462237-1462256	None:intergenic	30.0%
ACAACATCCAACTTGTATAT+TGG	+	chr2_3:1462250-1462269	Msa0270160:CDS	30.0%
TCATCCAAAATCATCTTACT+TGG	-	chr2_3:1462562-1462581	None:intergenic	30.0%
TGATTGTAGCTTATATCCTT+TGG	+	chr2_3:1462699-1462718	Msa0270160:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	1460487	1463530	1460487	ID=Msa0270160;Name=Msa0270160
chr2_3	mRNA	1460487	1463530	1460487	ID=Msa0270160-mRNA-1;Parent=Msa0270160;Name=Msa0270160-mRNA-1;_AED=0.04;_eAED=0.04;_QI=81\|1\|1\|1\|1\|1\|7\|310\|418
chr2_3	exon	1460487	1460579	1460487	ID=Msa0270160-mRNA-1:exon:579;Parent=Msa0270160-mRNA-1
chr2_3	exon	1460671	1460823	1460671	ID=Msa0270160-mRNA-1:exon:580;Parent=Msa0270160-mRNA-1
chr2_3	exon	1460912	1460972	1460912	ID=Msa0270160-mRNA-1:exon:581;Parent=Msa0270160-mRNA-1
chr2_3	exon	1461066	1461115	1461066	ID=Msa0270160-mRNA-1:exon:582;Parent=Msa0270160-mRNA-1
chr2_3	exon	1461552	1461653	1461552	ID=Msa0270160-mRNA-1:exon:583;Parent=Msa0270160-mRNA-1
chr2_3	exon	1462139	1462637	1462139	ID=Msa0270160-mRNA-1:exon:584;Parent=Msa0270160-mRNA-1
chr2_3	exon	1462841	1463530	1462841	ID=Msa0270160-mRNA-1:exon:585;Parent=Msa0270160-mRNA-1
chr2_3	five_prime_UTR	1460487	1460567	1460487	ID=Msa0270160-mRNA-1:five_prime_utr;Parent=Msa0270160-mRNA-1
chr2_3	CDS	1460568	1460579	1460568	ID=Msa0270160-mRNA-1:cds;Parent=Msa0270160-mRNA-1
chr2_3	CDS	1460671	1460823	1460671	ID=Msa0270160-mRNA-1:cds;Parent=Msa0270160-mRNA-1
chr2_3	CDS	1460912	1460972	1460912	ID=Msa0270160-mRNA-1:cds;Parent=Msa0270160-mRNA-1
chr2_3	CDS	1461066	1461115	1461066	ID=Msa0270160-mRNA-1:cds;Parent=Msa0270160-mRNA-1
chr2_3	CDS	1461552	1461653	1461552	ID=Msa0270160-mRNA-1:cds;Parent=Msa0270160-mRNA-1
chr2_3	CDS	1462139	1462637	1462139	ID=Msa0270160-mRNA-1:cds;Parent=Msa0270160-mRNA-1
chr2_3	CDS	1462841	1463220	1462841	ID=Msa0270160-mRNA-1:cds;Parent=Msa0270160-mRNA-1
chr2_3	three_prime_UTR	1463221	1463530	1463221	ID=Msa0270160-mRNA-1:three_prime_utr;Parent=Msa0270160-mRNA-1

Gene Sequence

>Msa0270160

ATGGCGGCCTTGATCCTTCACGCTCCCAACAACGGAAACAAAAATGTTTACAAAACTCTGATTGCTGCTGAATATGTTGGTGTTCAAGTTCAATTGACTCCTGATTTTCAGATGGGTGTTTCCAACAAAACCCCTCAATTTATCAACATGAACCCTATTGGAAAGGTCCCGGTCTTGGAAACTCCTGATGGTCCTGTCTTTGAGAGTAATGCCATTGCTCGCTATGTTGCCCGATTGGGTCACAACAATTTGTTTGGATCATCTCTTATTGATCAGGCCCACATTGACCAGTGGATTGATTTTTCTTCTCTGGAGATTGATGCCAATATCATGAAGTTGTACCTGCCAAGACTTGGATATGCCGCCTACTTTCCTCCAGTAGAGGAGAATGCAATTTCTTCGCTCAAGAGAGCACTTGAGGCATTGAACACTCATCTTGCTCACAATACCTACTTGGTCGGGCATTCTGTTACCCTTGCTGACATTATAACAACATCCAACTTGTATATTGGTTTCGCTAAACTCTTGGTTAAAAGCTTCACCTCAGAGTTTCCTCATGTTGAGAGATACTTTTGGACCTTGGTCAACCAGCCCAATTTCCGCAAGATATTAGGCCAGGTTAAGCAGACTGAGGCTATGCCACCTATTCCATCTGCTAAGCAGCAGCCTAAGGAGTCTAAGCCCAAAACCAAAGATGAACCAAAGAAAGTGGCTAAATCAGAGCCCGAAAAGCCCAAAGTAGAAGTTGAAGAGGAGGCACCCAAGCCCAAACCCAAGAATCCTCTTGACCTTCTTCCTCCAAGTAAGATGATTTTGGATGACTGGAAAAGACTCTACTCTAACACCAAGAGGAACTTCCGTGAGGTCGCAATCAAAGGATTCTGGGACATGTATGATCCAGAGGGATACTCTCTCTGGTTCTGTGATTACAAATACAATGATGAGAACACTGTTTCTTTTGTGACCCTGAACAAGGTTGGGGGATTTCTGCAGAGGATGGATCTGGCTCGCAAGTATGCTTTTGGAAAGATGCTTATAATTGGATCAGAGCCTCCATTTAAGGTGAAGGGGTTGTGGCTTTTCCGTGGACAAGAGATTCCAAAATTTATCATGGATGAATGCTATGACATGGAGCTCTATGAGTGGACCAAGGTTGACATCTCTGATGAAGTTCAGAAAGAGCGTGCCAGTCAGATGATTGAAGATGCTGAACCATTTGAGGGAGAGGCTCTTTTGGATGCCAAGTGCTTTAAGTGA

Protein sequence

>Msa0270160

MAALILHAPNNGNKNVYKTLIAAEYVGVQVQLTPDFQMGVSNKTPQFINMNPIGKVPVLETPDGPVFESNAIARYVARLGHNNLFGSSLIDQAHIDQWIDFSSLEIDANIMKLYLPRLGYAAYFPPVEENAISSLKRALEALNTHLAHNTYLVGHSVTLADIITTSNLYIGFAKLLVKSFTSEFPHVERYFWTLVNQPNFRKILGQVKQTEAMPPIPSAKQQPKESKPKTKDEPKKVAKSEPEKPKVEVEEEAPKPKPKNPLDLLPPSKMILDDWKRLYSNTKRNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLIIGSEPPFKVKGLWLFRGQEIPKFIMDECYDMELYEWTKVDISDEVQKERASQMIEDAEPFEGEALLDAKCFK*