AlfalfaGEDB | Msa1306580

M. sativa cultivar zhongmu-4 / Msa1306580

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009295 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010020 GO:0010468 GO:0016043 GO:0019222 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044424 GO:0044444 GO:0044464 GO:0048285 GO:0050789 GO:0060255 GO:0065007 GO:0071840
PFAM: PF01225.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1306580	sp\|F4I3P9\|MURE_ARATH	68.509	778	200	11	1	749	1	762	0.0	1006

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1306580	A0A072U5D4	97.680	776	18	0	1	776	1	776	0.0	1554

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa1306580	-0.816200	6.251501e-52	-8.615850e-47
Msa0001950	Msa1306580	0.813214	2.867034e-51	-8.615850e-47
Msa0027540	Msa1306580	0.802374	5.784194e-49	-8.615850e-47
Msa0029620	Msa1306580	0.811626	6.373588e-51	-8.615850e-47
Msa0030380	Msa1306580	0.811744	6.007453e-51	-8.615850e-47
Msa0044710	Msa1306580	0.831779	1.375988e-55	-8.615850e-47
Msa0050720	Msa1306580	0.815089	1.105168e-51	-8.615850e-47
Msa0067150	Msa1306580	0.805648	1.206163e-49	-8.615850e-47
Msa0075310	Msa1306580	0.805578	1.247833e-49	-8.615850e-47
Msa0102900	Msa1306580	0.822985	1.767139e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1306580	MtrunA17_Chr8g0386951	97.864	749	16	0	1	749	1	749	0.0	1500

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1306580	AT1G63680.1	68.509	778	200	11	1	749	1	762	1006
Msa1306580	AT1G63680.3	68.509	778	200	11	1	749	1	762	1006
Msa1306580	AT1G63680.2	68.509	778	200	11	1	749	1	762	1006

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Find 255 sgRNAs with CRISPR-Local

Find 405 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTATGTATGAAGCGAT+GGG	0.554179	8_4:-1542263	Msa1306580:CDS
AAAGAGAGTTCAAACTTCAA+GGG	0.587138	8_4:+1541831	None:intergenic
AAATCCCTGCATGTGAAAGC+TGG	0.432388	8_4:+1538972	None:intergenic
AAATGCTGTTCTTGCTACGT+TGG	0.568754	8_4:-1542375	Msa1306580:CDS
AAATTCCCGTAAACCGAAAC+AGG	0.415050	8_4:+1542602	None:intergenic
AACAGCTTCAATTTCGGATT+TGG	0.263849	8_4:+1542862	None:intergenic
AAGAGGTTGATGCAGTTCCT+GGG	0.546388	8_4:-1541656	Msa1306580:CDS
AAGCCTCCCTGCACTCTTCT+CGG	0.384444	8_4:+1539677	None:intergenic
AAGCGATGGGTTTACGTACC+GGG	0.504543	8_4:-1542250	Msa1306580:CDS
AAGGTTCGTCGGGAGCGTCT+TGG	0.443705	8_4:+1542990	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATGTGAACTGAAGAAGAAA+GGG	+	chr8_4:1538947-1538966	None:intergenic	30.0%
TTATGTGAACTGAAGAAGAA+AGG	+	chr8_4:1538948-1538967	None:intergenic	30.0%
CACATAATATACAGTCTGAA+TGG	-	chr8_4:1538961-1538980	Msa1306580:three_prime_UTR	30.0%
GCTAAACTCATCTTAAACTT+AGG	+	chr8_4:1539661-1539680	None:intergenic	30.0%
ATGGAATTCAACATGATTCT+AGG	-	chr8_4:1539746-1539765	Msa1306580:intron	30.0%
TAAGTATCCTTCGAAAAACA+TGG	-	chr8_4:1539972-1539991	Msa1306580:intron	30.0%
AAAGAGAGTTCAAACTTCAA+GGG	+	chr8_4:1540483-1540502	None:intergenic	30.0%
AGAATCAAGTAATCTAGACA+AGG	+	chr8_4:1540748-1540767	None:intergenic	30.0%
TTTCTCTTAATTGATGTGGA+AGG	-	chr8_4:1540813-1540832	Msa1306580:intron	30.0%
AAAAGAAGAAAAAAGACGGT+CGG	+	chr8_4:1540973-1540992	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	1538912	1543421	1538912	ID=Msa1306580;Name=Msa1306580
chr8_4	mRNA	1538912	1543421	1538912	ID=Msa1306580-mRNA-1;Parent=Msa1306580;Name=Msa1306580-mRNA-1;_AED=0.01;_eAED=0.01;_QI=68\|0.75\|0.8\|1\|1\|1\|5\|469\|776
chr8_4	exon	1538912	1539466	1538912	ID=Msa1306580-mRNA-1:exon:418;Parent=Msa1306580-mRNA-1
chr8_4	exon	1539635	1539764	1539635	ID=Msa1306580-mRNA-1:exon:417;Parent=Msa1306580-mRNA-1
chr8_4	exon	1540896	1541071	1540896	ID=Msa1306580-mRNA-1:exon:416;Parent=Msa1306580-mRNA-1
chr8_4	exon	1541220	1541336	1541220	ID=Msa1306580-mRNA-1:exon:415;Parent=Msa1306580-mRNA-1
chr8_4	exon	1541532	1543421	1541532	ID=Msa1306580-mRNA-1:exon:414;Parent=Msa1306580-mRNA-1
chr8_4	five_prime_UTR	1543354	1543421	1543354	ID=Msa1306580-mRNA-1:five_prime_utr;Parent=Msa1306580-mRNA-1
chr8_4	CDS	1541532	1543353	1541532	ID=Msa1306580-mRNA-1:cds;Parent=Msa1306580-mRNA-1
chr8_4	CDS	1541220	1541336	1541220	ID=Msa1306580-mRNA-1:cds;Parent=Msa1306580-mRNA-1
chr8_4	CDS	1540896	1541071	1540896	ID=Msa1306580-mRNA-1:cds;Parent=Msa1306580-mRNA-1
chr8_4	CDS	1539635	1539764	1539635	ID=Msa1306580-mRNA-1:cds;Parent=Msa1306580-mRNA-1
chr8_4	CDS	1539381	1539466	1539381	ID=Msa1306580-mRNA-1:cds;Parent=Msa1306580-mRNA-1
chr8_4	three_prime_UTR	1538912	1539380	1538912	ID=Msa1306580-mRNA-1:three_prime_utr;Parent=Msa1306580-mRNA-1

Gene Sequence

>Msa1306580

ATGGCTTTGCCATTTGTTACTCTTCCACACTTCATTTCTCCTACAACTATCAAACCCAATTCAACCTTCACCATTCTAAAACCCAAACACTTCCTTCTTCAATTCCCTTCCCGTCCCTTATCTTATCCCTTACTTCCTCTCCGCCCTCCCTCCGCCATCGCACCCGACGGAAAATTCTATCCAAACCCAGCTGACGACGACCCACCCGAAGTAGGCGAAGATTCCGCTCACGGCTTCACCAAATACCAACAAATCCAACTCCAAGCCGACCGCGCTCGCCTTCGTGAAGAAGAAGATTTCAAAAACAATCAAGCCACTTACCTCGCCGCCATCGCCGACTCCCAAGACGCTCCCGACGAACCTTCCGCCACTGATTCCGCCGAAGACGATTTATTCGGCGAAATAGATAAAGCTATCGCTCTTAAACGTAAAGAGTTCGTTAAGCAAGGTCTTCTTAAACCTAATCCCGCCAAATCCGAAATTGAAGCTGTTGAAGAGCTTCAACCTGATGAAGTCGACGATGTAGAAGAAATTGAACGTCTCCGTGAACTCACTGTGAATTCTGATGAATCTCAAGGAGATTTTGAAGAAGATGGTGATTTATCTTTGAAATCCGAATCTTCCTTTGATTTAGATTTTGATAGTTTAGGGAAAAGTAATAAAGCTAGAATTGTTGAACCTAAGTTTAAGATGAGTTTAGCTGAGCTTTTGGATGAGAGTAAAGTGGTTCCTGTTTCGGTTTACGGGAATTTGGAAGTTGAAATTAATGGAATTCAACATGATTCTAGGCTTGTTACTTCTGGTAATTTGTTTGTGTGTTGTGTTGGAAGAAAAAACGATGGACATATGTTTTTGAGTGAGGCTGATAAGAGAGGTGCTATTGCTGTTGTAGCGAGTAAAGAGATTGATATTGAAGATACTTTAGGTTGTAAGGCTTTGGTTATTGTGGAAGATACAAATGCTGTTCTTGCTACGTTGGCTGCGTCGTTTTATAAGTATCCTTCGAAAAACATGGCTCTTATTGGGATAACGGGGACTTATGGTAAGACAACGACGACTTATTTGATTAAAAGTATGTATGAAGCGATGGGTTTACGTACCGGGATGTTTAACTCGATTGCTAGTTATGTTCATGGGGATAATCAGTTGGATTCGTCTTATGGGGTTCTGGATGCTGTTTTGGTTCAGAATTTGATGGCTAAGATGCTTCATAATGGAACTGAATCTGTGGTTTTTGAGGCTTCTTCGCGCGGATTGGCTCAGGGGAAATATGATGAGGTTGATTTTGATGTTGCTGTTTTTACGAATTTGATGGATGAGGAGGACGAGGAAGAGGATAGAGATGCGAAGGCTAAGCTGTTTTCAAGGATGGTGGATCCTGAGCGGCATAGGAAAGTTGTTAACATTGACGATCCAAGTGCGCCTTTGTTCGTTTCAATGGGGAGCCCTGAAGTTCCTGTTGTGACATTTGCGTTGGAGAATAAGAATGCAGATGTTCATCCCTTGAAGTTTGAACTCTCTTTGTTTGAGACACAGGTGTTGGTTAATACACCTGCTGGGATACTGGAAATTTCGTCCGGTTTACTAGGAAGGCACAATGTTTACAACATTCTTGCTGCTGTGGCAGTTGGCATTGCTGTTGGGGCACCCTTGGAGGATATTGTTAGAGGGATTGAAGAGGTTGATGCAGTTCCTGGGAGGTGTGAGTTAATTGACGAGGAACAAGCATTTGGGGTCATAGTGGACTATGCTCGGACTCCTGATGCCTTGTCTAGATTACTTGATTCTGTAAGAGAGCTTCAACCTCGCAGGATTATAACTGTGATTGGTTGCTGTGGTGAGGACGAAAGGGGGAAGAGACCCCTGATGACAAAGATAGCAACAGATAAAAGTGAAGTGACCATGCTGACATCCGACAACCCCAAGGGTGAAGATCCATTGGATATTTTGGACGATATGTTGGCTGGGGTAGGATGGACAATGCAAGAGTATCTGAAATATGGAGAGAATGACTACTATCCTCCTCTTTCAAATGGTCACAGGCTTTTTCTCCATGACATTAGGAGGGTAGCTGTGCGAGCTTCAGTTGCAATGGGGGAGGAGGGTGATGTTGTTGTGATTGCTGGTAAAGGCCATGAAACATATCAAATAGAAGGTGAGAAAAAGGATTTCTTCGATGACCGAGAAGAGTGCAGGGAGGCTTTGCAGTATGTTGACGAGCTTCATCAAGCTGGAATAGATACAAGTTACCAGAGAGCCACTGATACCAAAGGTTGCTGGGCTAACAATAGATCAGCTTCACCTGTTCACCTGAGTACACAAATGAAGTAG

Protein sequence

>Msa1306580

MALPFVTLPHFISPTTIKPNSTFTILKPKHFLLQFPSRPLSYPLLPLRPPSAIAPDGKFYPNPADDDPPEVGEDSAHGFTKYQQIQLQADRARLREEEDFKNNQATYLAAIADSQDAPDEPSATDSAEDDLFGEIDKAIALKRKEFVKQGLLKPNPAKSEIEAVEELQPDEVDDVEEIERLRELTVNSDESQGDFEEDGDLSLKSESSFDLDFDSLGKSNKARIVEPKFKMSLAELLDESKVVPVSVYGNLEVEINGIQHDSRLVTSGNLFVCCVGRKNDGHMFLSEADKRGAIAVVASKEIDIEDTLGCKALVIVEDTNAVLATLAASFYKYPSKNMALIGITGTYGKTTTTYLIKSMYEAMGLRTGMFNSIASYVHGDNQLDSSYGVLDAVLVQNLMAKMLHNGTESVVFEASSRGLAQGKYDEVDFDVAVFTNLMDEEDEEEDRDAKAKLFSRMVDPERHRKVVNIDDPSAPLFVSMGSPEVPVVTFALENKNADVHPLKFELSLFETQVLVNTPAGILEISSGLLGRHNVYNILAAVAVGIAVGAPLEDIVRGIEEVDAVPGRCELIDEEQAFGVIVDYARTPDALSRLLDSVRELQPRRIITVIGCCGEDERGKRPLMTKIATDKSEVTMLTSDNPKGEDPLDILDDMLAGVGWTMQEYLKYGENDYYPPLSNGHRLFLHDIRRVAVRASVAMGEEGDVVVIAGKGHETYQIEGEKKDFFDDREECREALQYVDELHQAGIDTSYQRATDTKGCWANNRSASPVHLSTQMK*