Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0067150 | XP_013467320.1 | 85.097 | 463 | 11 | 3 | 1 | 462 | 1 | 406 | 0.0 | 775 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0067150 | sp|Q9FNB2|RRL_ARATH | 77.632 | 228 | 51 | 0 | 231 | 458 | 174 | 401 | 5.08e-126 | 374 |
| Msa0067150 | sp|Q9FNB2|RRL_ARATH | 69.811 | 53 | 16 | 0 | 113 | 165 | 113 | 165 | 3.38e-16 | 83.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0067150 | A0A072VHW2 | 85.097 | 463 | 11 | 3 | 1 | 462 | 1 | 406 | 0.0 | 775 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000490 | Msa0067150 | -0.837826 | 4.133613e-57 | -8.615850e-47 |
| Msa0003470 | Msa0067150 | 0.816921 | 4.310578e-52 | -8.615850e-47 |
| Msa0006700 | Msa0067150 | 0.811942 | 5.439186e-51 | -8.615850e-47 |
| Msa0006820 | Msa0067150 | 0.802337 | 5.885889e-49 | -8.615850e-47 |
| Msa0008850 | Msa0067150 | 0.871502 | 6.508702e-67 | -8.615850e-47 |
| Msa0013050 | Msa0067150 | -0.801440 | 8.995183e-49 | -8.615850e-47 |
| Msa0014410 | Msa0067150 | 0.809248 | 2.077921e-50 | -8.615850e-47 |
| Msa0018470 | Msa0067150 | 0.860425 | 2.084962e-63 | -8.615850e-47 |
| Msa0020630 | Msa0067150 | 0.839879 | 1.217070e-57 | -8.615850e-47 |
| Msa0027540 | Msa0067150 | 0.849362 | 3.394171e-60 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0067150 | MtrunA17_Chr1g0170791 | 85.097 | 463 | 11 | 3 | 1 | 462 | 1 | 406 | 0.0 | 775 |
| Msa0067150 | MtrunA17_Chr5g0418301 | 51.200 | 375 | 119 | 6 | 87 | 458 | 95 | 408 | 2.73e-118 | 352 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0067150 | AT5G13610.1 | 77.632 | 228 | 51 | 0 | 231 | 458 | 174 | 401 | 5.17e-127 | 374 |
| Msa0067150 | AT5G13610.1 | 69.811 | 53 | 16 | 0 | 113 | 165 | 113 | 165 | 3.43e-17 | 83.6 |
| Msa0067150 | AT1G69380.1 | 65.487 | 226 | 78 | 0 | 234 | 459 | 144 | 369 | 9.13e-104 | 314 |
Find 93 sgRNAs with CRISPR-Local
Find 658 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAACGAAGGAGTGAGG+TGG | 0.673089 | 1_2:+31863285 | None:intergenic |
| AAAGAGTGAGATTGTGATTT+TGG | 0.142702 | 1_2:+31863187 | None:intergenic |
| AACGCTCTAACAGGAATTGC+AGG | 0.396685 | 1_2:+31862974 | None:intergenic |
| AATCGTGAGATGGAAGCGAC+TGG | 0.588881 | 1_2:-31854822 | Msa0067150:CDS |
| AATTATGTAGTTCTCAAGTT+TGG | 0.333870 | 1_2:-31860548 | Msa0067150:CDS |
| AATTCATTTGAATCATCCAG+TGG | 0.652066 | 1_2:+31859578 | None:intergenic |
| AATTTGCCTTGCCCACCAAC+TGG | 0.414032 | 1_2:+31854769 | None:intergenic |
| ACCATATTTGGCATCTTTCC+AGG | 0.288418 | 1_2:+31853891 | None:intergenic |
| ACGCAAACATGGGAAGAAGA+AGG | 0.603139 | 1_2:+31863373 | None:intergenic |
| ACTAAACTACTAAAGAACCG+AGG | 0.718924 | 1_2:+31853209 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGAAAAAAGGGTTTAGCAT+AGG | - | chr1_2:31853186-31853205 | Msa0067150:three_prime_UTR | 30.0% | |
| AGTAAAATTTAACCGAGTCA+GGG | + | chr1_2:31854294-31854313 | None:intergenic | 30.0% | |
| GTCATTTGATTGTAATGTTC+GGG | - | chr1_2:31854321-31854340 | Msa0067150:intron | 30.0% | |
| TTGACCGTTTAATAGAATGA+GGG | - | chr1_2:31854446-31854465 | Msa0067150:intron | 30.0% | |
| AATTTGTCTGTGACTTTGTA+AGG | + | chr1_2:31854516-31854535 | None:intergenic | 30.0% | |
| TACAAAGTCACAGACAAATT+TGG | - | chr1_2:31854516-31854535 | Msa0067150:intron | 30.0% | |
| TCAATTGTGGTAGATATATC+TGG | - | chr1_2:31854658-31854677 | Msa0067150:intron | 30.0% | |
| GTAAATCTTATTAGTGCCTT+AGG | - | chr1_2:31855294-31855313 | Msa0067150:intron | 30.0% | |
| CTATTATTTAAACCACTCCT+CGG | - | chr1_2:31855499-31855518 | Msa0067150:intron | 30.0% | |
| AAATATCACGTATTTGCCTA+TGG | - | chr1_2:31855554-31855573 | Msa0067150:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 31853133 | 31863494 | 31853133 | ID=Msa0067150;Name=Msa0067150 |
| chr1_2 | mRNA | 31853133 | 31863494 | 31853133 | ID=Msa0067150-mRNA-1;Parent=Msa0067150;Name=Msa0067150-mRNA-1;_AED=0.20;_eAED=0.20;_QI=144|0.71|0.75|0.87|1|1|8|430|462 |
| chr1_2 | exon | 31853133 | 31853700 | 31853133 | ID=Msa0067150-mRNA-1:exon:10469;Parent=Msa0067150-mRNA-1 |
| chr1_2 | exon | 31853810 | 31853924 | 31853810 | ID=Msa0067150-mRNA-1:exon:10468;Parent=Msa0067150-mRNA-1 |
| chr1_2 | exon | 31854726 | 31854877 | 31854726 | ID=Msa0067150-mRNA-1:exon:10467;Parent=Msa0067150-mRNA-1 |
| chr1_2 | exon | 31854959 | 31855107 | 31854959 | ID=Msa0067150-mRNA-1:exon:10466;Parent=Msa0067150-mRNA-1 |
| chr1_2 | exon | 31859333 | 31859627 | 31859333 | ID=Msa0067150-mRNA-1:exon:10465;Parent=Msa0067150-mRNA-1 |
| chr1_2 | exon | 31860381 | 31860419 | 31860381 | ID=Msa0067150-mRNA-1:exon:10464;Parent=Msa0067150-mRNA-1 |
| chr1_2 | exon | 31860529 | 31860637 | 31860529 | ID=Msa0067150-mRNA-1:exon:10463;Parent=Msa0067150-mRNA-1 |
| chr1_2 | exon | 31862959 | 31863494 | 31862959 | ID=Msa0067150-mRNA-1:exon:10462;Parent=Msa0067150-mRNA-1 |
| chr1_2 | five_prime_UTR | 31863351 | 31863494 | 31863351 | ID=Msa0067150-mRNA-1:five_prime_utr;Parent=Msa0067150-mRNA-1 |
| chr1_2 | CDS | 31862959 | 31863350 | 31862959 | ID=Msa0067150-mRNA-1:cds;Parent=Msa0067150-mRNA-1 |
| chr1_2 | CDS | 31860529 | 31860637 | 31860529 | ID=Msa0067150-mRNA-1:cds;Parent=Msa0067150-mRNA-1 |
| chr1_2 | CDS | 31860381 | 31860419 | 31860381 | ID=Msa0067150-mRNA-1:cds;Parent=Msa0067150-mRNA-1 |
| chr1_2 | CDS | 31859333 | 31859627 | 31859333 | ID=Msa0067150-mRNA-1:cds;Parent=Msa0067150-mRNA-1 |
| chr1_2 | CDS | 31854959 | 31855107 | 31854959 | ID=Msa0067150-mRNA-1:cds;Parent=Msa0067150-mRNA-1 |
| chr1_2 | CDS | 31854726 | 31854877 | 31854726 | ID=Msa0067150-mRNA-1:cds;Parent=Msa0067150-mRNA-1 |
| chr1_2 | CDS | 31853810 | 31853924 | 31853810 | ID=Msa0067150-mRNA-1:cds;Parent=Msa0067150-mRNA-1 |
| chr1_2 | CDS | 31853563 | 31853700 | 31853563 | ID=Msa0067150-mRNA-1:cds;Parent=Msa0067150-mRNA-1 |
| chr1_2 | three_prime_UTR | 31853133 | 31853562 | 31853133 | ID=Msa0067150-mRNA-1:three_prime_utr;Parent=Msa0067150-mRNA-1 |
| Gene Sequence |
>Msa0067150 ATGTTACGAAGCATAGAAACCACTCATCACCTCACTGTTAGAACCACCTCACTCCTTCGTTCTTTTCTCTTCTCCTCTCCTTCACCAAACACTTCTTCTTATTCTTCTTTCACTTTCAAAACATTCACCACTCTCTCTAAACCAAAATCACAATCTCACTCTTTTCTTCTTCAGCAACGAGAACGCGCTTCTTCCTTCATCACTCGTCGTTTTCATTTCGCTTCTTATGTTAAACGCCGCGTTTCGTCATCGTCGTTTCCTGCACTTGATTGGAACGACGCCGTTTCATGCTCTGAGGTTGATGTTGATGCTGATAATGTTAATGATAATGGAACAATTGATCAGGATTCTAAACCTGCAATTCCTGTTAGAGCGTTCTTCTTCTCCACCAGTGTTGATTTAAAAAGCTTGGTGGAGCAGAACAAACCAAACTTTGTCACACCATCATCAAGGATGACTAATTATGTAGTTCTCAAGTTTGGCAATCTTGGCGATTCCAAGTTCTATATATGTCCTGTAAGACGCGGAGTGAATAAAGATGCTGTTATATCTGTGAAAAGGTTTCATCCACTGGATGATTCAAATGAATTGTCTGCCTCTTTCTCAGCTGTTACATATAATTTTGAAGTCATAGGTCTGTTGATACTAATTGCAAAATTCTTTTTGTTTCAGGGTCCTGGTTCTTCTTTCTTGAATGGAACAAATGGCTGTTACATGGTAGTTTTTCAGTATGGCTCCATTGTATTGTTTAATGTCCTCGAACATGAGGTTGATGGCTATTTGAAAATTGTTAGGAAGCATGCATCTGGTTTGCTTCCTGAAATGAGAAAGGATGAGTATGAAGTACGAGAAAAACCAGCTTTGAGCACATGGATGCAAGGAGGACTGGATTACATAATGTTACAATACTTGGATGTTGATGGAATTCGAACTATTGGTAGTGTACTAGGACAAAGTATTGCTCTTGATTACTATGGCCGGCAGGTTGATGGAATGGTTGCAGAATTTACAGACATAAATCGTGAGATGGAAGCGACTGGAAAATTTAAGATGCAAAGGAAAAAACTTTTCCAGTTGGTGGGCAAGGCAAATTCAAATCTTGCTGATGTGATTCTAAAGCTCGGACTATTTGAGAGATCAGATATCGCCTGGAAAGATGCCAAATATGGTCAGATATGGGAGTATCTCAGGGACGAATTTGAGTTAACTCAGAGATTTGCAAGTCTTGATTTCAAGTTGAAATTTGTGGAGCACAATATTCGATTTCTACAAGAAATTCTTCAAAACAGGAAATCTGATTTCCTTGAGTGGCTCATTATTGCATTGATTGGTGCTGAAATTCTGCTGTCTCTATATGACATTGTTCAAAGGTCTGCGATGAATCTTTAA |
| Protein sequence |
>Msa0067150 MLRSIETTHHLTVRTTSLLRSFLFSSPSPNTSSYSSFTFKTFTTLSKPKSQSHSFLLQQRERASSFITRRFHFASYVKRRVSSSSFPALDWNDAVSCSEVDVDADNVNDNGTIDQDSKPAIPVRAFFFSTSVDLKSLVEQNKPNFVTPSSRMTNYVVLKFGNLGDSKFYICPVRRGVNKDAVISVKRFHPLDDSNELSASFSAVTYNFEVIGLLILIAKFFLFQGPGSSFLNGTNGCYMVVFQYGSIVLFNVLEHEVDGYLKIVRKHASGLLPEMRKDEYEVREKPALSTWMQGGLDYIMLQYLDVDGIRTIGSVLGQSIALDYYGRQVDGMVAEFTDINREMEATGKFKMQRKKLFQLVGKANSNLADVILKLGLFERSDIAWKDAKYGQIWEYLRDEFELTQRFASLDFKLKFVEHNIRFLQEILQNRKSDFLEWLIIALIGAEILLSLYDIVQRSAMNL* |
