AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1325800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1325800 MtrunA17_Chr8g0360351 97.163 282 8 0 1 282 1 282 0.0 562
Msa1325800 MtrunA17_Chr2g0317021 79.505 283 57 1 1 282 51 333 5.19e-165 461
Msa1325800 MtrunA17_Chr4g0050381 65.038 266 92 1 16 281 12 276 1.08e-120 346
Msa1325800 MtrunA17_Chr2g0279151 61.255 271 104 1 11 281 11 280 3.82e-113 327
Msa1325800 MtrunA17_Chr5g0426141 61.887 265 100 1 18 282 19 282 9.93e-112 323
Msa1325800 MtrunA17_Chr3g0115851 59.480 269 108 1 15 282 14 282 3.68e-111 322
Msa1325800 MtrunA17_Chr4g0051661 56.705 261 112 1 21 281 138 397 1.81e-101 301
Msa1325800 MtrunA17_Chr1g0212961 43.233 266 138 7 22 281 79 337 2.47e-65 207
Msa1325800 MtrunA17_Chr1g0150451 43.369 279 142 7 14 281 50 323 2.18e-64 205
Msa1325800 MtrunA17_Chr3g0136711 44.528 265 139 6 20 281 21 280 1.53e-63 201
Msa1325800 MtrunA17_Chr3g0126511 40.784 255 144 5 31 282 91 341 1.59e-54 180
Msa1325800 MtrunA17_Chr3g0105371 37.838 259 143 8 36 282 87 339 1.12e-39 141
Msa1325800 MtrunA17_Chr5g0430481 37.354 257 145 7 35 281 124 374 2.26e-39 141
Msa1325800 MtrunA17_Chr1g0187481 34.766 256 141 6 33 281 41 277 3.43e-39 138
Msa1325800 MtrunA17_Chr1g0166481 37.891 256 129 6 33 281 41 273 5.52e-39 137
Msa1325800 MtrunA17_Chr3g0105351 37.452 259 144 8 36 282 63 315 1.57e-38 137
Msa1325800 MtrunA17_Chr4g0065141 33.607 244 139 6 56 282 114 351 7.29e-34 126
Msa1325800 MtrunA17_Chr5g0407161 33.624 229 134 6 65 281 123 345 1.32e-31 120
Msa1325800 MtrunA17_Chr4g0070871 35.039 254 120 8 66 279 253 501 3.81e-30 118
Msa1325800 MtrunA17_Chr3g0112291 34.559 272 135 7 48 279 105 373 7.73e-30 115
Msa1325800 MtrunA17_Chr3g0138281 33.610 241 138 7 57 282 113 346 1.57e-29 115
Msa1325800 MtrunA17_Chr1g0171571 34.818 247 144 7 47 279 399 642 1.62e-29 117
Msa1325800 MtrunA17_Chr3g0135961 34.375 256 122 7 66 279 286 537 2.59e-29 116
Msa1325800 MtrunA17_Chr5g0396731 34.440 241 120 9 34 249 72 299 1.28e-28 112
Msa1325800 MtrunA17_Chr8g0389921 33.613 238 131 6 66 279 198 432 2.60e-28 112
Msa1325800 MtrunA17_Chr6g0483411 32.222 270 145 11 45 281 54 318 4.60e-28 110
Msa1325800 MtrunA17_Chr2g0293811 31.356 236 134 8 66 279 100 329 5.33e-28 110
Msa1325800 MtrunA17_Chr2g0299981 31.343 335 154 13 17 279 203 533 4.83e-27 110
Msa1325800 MtrunA17_Chr1g0194191 30.380 237 137 9 65 279 117 347 1.65e-26 107
Msa1325800 MtrunA17_Chr3g0144111 32.895 228 114 8 66 281 27 227 2.22e-26 104
Msa1325800 MtrunA17_Chr5g0435721 35.319 235 125 8 35 249 193 420 2.86e-26 107
Msa1325800 MtrunA17_Chr1g0158781 35.409 257 137 8 48 279 125 377 7.24e-25 102
Msa1325800 MtrunA17_Chr1g0150781 33.725 255 125 8 66 279 290 541 7.48e-25 103
Msa1325800 MtrunA17_Chr1g0192421 32.624 282 132 13 52 282 77 351 1.33e-23 98.6
Msa1325800 MtrunA17_Chr3g0127091 28.897 263 128 9 68 278 91 346 5.54e-23 97.1
Msa1325800 MtrunA17_Chr1g0150071 26.959 319 154 12 32 281 21 329 1.45e-22 95.5
Msa1325800 MtrunA17_Chr7g0256771 27.304 293 155 10 19 262 26 309 2.90e-22 94.0
Msa1325800 MtrunA17_Chr7g0225501 35.294 238 128 13 64 279 84 317 1.63e-21 93.6
Msa1325800 MtrunA17_Chr7g0271971 31.780 236 131 10 66 279 68 295 2.17e-21 93.2
Msa1325800 MtrunA17_Chr2g0295401 32.588 313 151 19 22 282 50 354 3.75e-21 91.7
Msa1325800 MtrunA17_Chr1g0211251 27.612 268 141 8 31 250 42 304 4.37e-21 92.0
Msa1325800 MtrunA17_Chr7g0272061 30.741 270 130 12 69 282 94 362 2.94e-20 89.4
Msa1325800 MtrunA17_Chr7g0263281 25.545 321 168 10 29 282 30 346 3.64e-20 89.0
Msa1325800 MtrunA17_Chr4g0001761 34.857 175 89 5 126 281 160 328 1.68e-19 87.0
Msa1325800 MtrunA17_Chr1g0153671 30.584 291 161 14 10 279 22 292 1.69e-19 87.8
Msa1325800 MtrunA17_Chr4g0067711 37.705 183 88 11 124 282 173 353 1.77e-19 87.0
Msa1325800 MtrunA17_Chr3g0136811 32.955 176 92 5 126 281 160 329 1.97e-19 87.0
Msa1325800 MtrunA17_Chr1g0192471 27.897 233 144 7 66 279 68 295 5.32e-19 86.3
Msa1325800 MtrunA17_Chr4g0063111 27.273 275 153 11 39 275 51 316 6.43e-19 86.3
Msa1325800 MtrunA17_Chr3g0133801 31.915 235 132 12 66 279 66 293 8.43e-19 85.5
Msa1325800 MtrunA17_Chr1g0209451 27.451 255 156 10 49 279 46 295 2.11e-18 84.3
Msa1325800 MtrunA17_Chr1g0213351 27.336 289 150 11 29 266 39 318 4.33e-18 83.2
Msa1325800 MtrunA17_Chr7g0250061 26.667 285 153 11 31 266 37 314 5.69e-18 83.2
Msa1325800 MtrunA17_Chr1g0181901 26.766 269 146 10 29 250 35 299 9.75e-18 82.4
Msa1325800 MtrunA17_Chr7g0256751 27.864 323 153 12 29 281 35 347 9.86e-18 82.8
Msa1325800 MtrunA17_Chr4g0066371 27.059 255 145 9 66 280 86 339 1.37e-17 82.0
Msa1325800 MtrunA17_Chr8g0371321 27.143 280 145 10 31 259 40 311 1.45e-17 82.0
Msa1325800 MtrunA17_Chr8g0342431 37.791 172 84 8 133 282 203 373 6.72e-17 80.1
Msa1325800 MtrunA17_Chr7g0221691 37.576 165 69 7 126 266 232 386 8.86e-17 79.7
Msa1325800 MtrunA17_Chr7g0243571 37.179 156 82 6 126 266 188 342 9.33e-17 79.7
Msa1325800 MtrunA17_Chr3g0089721 27.778 306 166 12 6 266 11 306 1.29e-16 79.0
Msa1325800 MtrunA17_Chr3g0089711 27.778 306 166 12 6 266 11 306 1.57e-16 78.6
Msa1325800 MtrunA17_Chr7g0249421 33.854 192 109 7 92 266 172 362 2.47e-16 78.6
Msa1325800 MtrunA17_Chr4g0004971 38.608 158 80 7 125 266 165 321 2.56e-16 78.2
Msa1325800 MtrunA17_Chr4g0022171 35.028 177 97 8 106 265 172 347 1.12e-15 76.6
Msa1325800 MtrunA17_Chr1g0207571 27.018 285 152 11 29 267 28 302 1.28e-15 76.3
Msa1325800 MtrunA17_Chr2g0324061 35.294 187 100 8 96 265 158 340 1.16e-14 73.6
Msa1325800 MtrunA17_Chr4g0063051 28.916 249 145 10 37 266 35 270 1.07e-13 70.1
Msa1325800 MtrunA17_Chr2g0294291 26.596 282 156 12 29 267 42 315 3.61e-13 68.9
Msa1325800 MtrunA17_Chr1g0175381 25.000 316 161 12 29 279 46 350 4.17e-13 68.9
Msa1325800 MtrunA17_Chr2g0295991 25.625 320 133 11 60 279 160 474 1.22e-12 67.8
Msa1325800 MtrunA17_Chr1g0151781 34.247 146 64 6 126 248 152 288 1.54e-12 67.0
Msa1325800 MtrunA17_Chr4g0068971 31.343 134 65 4 173 279 96 229 3.34e-12 65.1
Msa1325800 MtrunA17_Chr7g0238501 25.694 288 150 10 33 267 235 511 4.24e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1325800 AT3G15260.2 74.740 289 66 2 1 282 1 289 1.80e-159 445
Msa1325800 AT3G15260.1 74.740 289 66 2 1 282 1 289 1.80e-159 445
Msa1325800 AT4G28400.1 66.784 283 93 1 1 282 1 283 1.44e-139 395
Msa1325800 AT4G28400.3 68.462 260 82 0 23 282 2 261 3.88e-134 380
Msa1325800 AT2G20630.1 63.121 282 101 1 1 282 1 279 1.59e-131 374
Msa1325800 AT2G20630.2 62.633 281 102 1 1 281 1 278 1.04e-128 367
Msa1325800 AT4G28400.2 65.690 239 81 1 1 238 1 239 1.49e-113 328
Msa1325800 AT1G22280.1 60.800 250 98 0 33 282 32 281 1.03e-111 324
Msa1325800 AT1G34750.2 59.480 269 108 1 14 282 15 282 1.21e-110 321
Msa1325800 AT1G34750.1 59.480 269 108 1 14 282 15 282 1.21e-110 321
Msa1325800 AT1G34750.3 59.480 269 108 1 14 282 15 282 1.21e-110 321
Msa1325800 AT1G22280.3 59.375 256 98 1 33 282 32 287 2.85e-109 318
Msa1325800 AT1G78200.1 54.579 273 120 3 13 282 12 283 1.21e-97 288
Msa1325800 AT1G78200.3 54.579 273 120 3 13 282 12 283 1.21e-97 288
Msa1325800 AT1G78200.2 54.579 273 120 3 13 282 12 283 1.21e-97 288
Msa1325800 AT2G34740.1 58.233 249 100 3 35 281 88 334 1.59e-95 285
Msa1325800 AT1G34750.4 59.111 225 91 1 14 238 15 238 4.04e-90 268
Msa1325800 AT1G78200.5 57.143 238 99 2 48 282 1 238 3.56e-89 265
Msa1325800 AT1G78200.4 57.143 238 99 2 48 282 1 238 3.56e-89 265
Msa1325800 AT2G34740.2 57.143 210 86 3 35 242 102 309 2.16e-79 243
Msa1325800 AT2G34740.3 57.143 210 86 3 35 242 84 291 2.21e-79 243
Msa1325800 AT1G22280.2 61.039 154 60 0 33 186 32 185 5.19e-65 202
Msa1325800 AT5G10740.1 43.774 265 141 6 20 281 20 279 3.88e-64 205
Msa1325800 AT5G10740.2 43.774 265 141 6 20 281 20 279 3.88e-64 205
Msa1325800 AT4G31750.2 43.019 265 143 6 20 281 20 279 1.04e-62 200
Msa1325800 AT4G31750.1 43.019 265 143 6 20 281 20 279 1.53e-62 200
Msa1325800 AT4G31750.3 43.019 265 143 6 20 281 20 279 1.53e-62 200
Msa1325800 AT5G24940.1 43.019 265 143 6 20 281 20 279 2.78e-62 203
Msa1325800 AT1G43900.1 42.460 252 137 6 34 282 123 369 2.75e-61 198
Msa1325800 AT5G53140.1 37.945 253 150 5 33 282 100 348 9.29e-51 172
Msa1325800 AT5G53140.2 37.945 253 150 5 33 282 100 348 9.29e-51 172
Msa1325800 AT1G07160.1 37.906 277 155 7 18 281 104 376 4.36e-42 149
Msa1325800 AT2G30020.1 40.650 246 131 6 45 279 149 390 1.39e-40 145
Msa1325800 AT2G40180.1 35.857 251 140 7 48 282 141 386 3.79e-34 128
Msa1325800 AT3G51470.2 31.765 255 145 7 46 281 39 283 4.58e-33 123
Msa1325800 AT3G51470.1 32.157 255 144 8 46 281 83 327 1.29e-32 123
Msa1325800 AT1G67820.2 37.089 213 124 6 59 266 108 315 9.23e-32 122
Msa1325800 AT1G67820.1 37.089 213 124 6 59 266 144 351 2.28e-31 121
Msa1325800 AT2G25620.1 34.615 234 128 7 65 279 127 354 4.90e-31 119
Msa1325800 AT2G25620.2 34.615 234 128 7 65 279 127 354 4.90e-31 119
Msa1325800 AT3G23360.1 31.225 253 140 6 33 281 37 259 8.45e-31 116
Msa1325800 AT2G33700.1 33.188 229 135 6 65 281 122 344 1.75e-30 118
Msa1325800 AT3G62260.3 32.636 239 134 8 65 281 26 259 3.99e-30 115
Msa1325800 AT5G51760.2 37.175 269 111 9 66 279 114 379 2.23e-29 115
Msa1325800 AT5G51760.1 37.175 269 111 9 66 279 144 409 3.02e-29 115
Msa1325800 AT3G62260.2 32.922 243 128 8 65 281 116 349 3.03e-29 114
Msa1325800 AT3G62260.1 32.922 243 128 8 65 281 115 348 3.05e-29 114
Msa1325800 AT3G11410.1 37.321 209 96 6 66 245 137 339 3.10e-29 115
Msa1325800 AT1G07430.1 36.816 201 106 5 67 249 158 355 2.01e-28 113
Msa1325800 AT5G59220.1 38.776 196 102 5 69 249 151 343 3.04e-28 112
Msa1325800 AT1G48040.1 31.535 241 135 6 65 281 115 349 3.38e-28 112
Msa1325800 AT2G29380.1 33.103 290 140 12 35 279 78 358 3.73e-28 111
Msa1325800 AT3G16800.4 36.680 259 114 14 69 282 1 254 8.35e-28 108
Msa1325800 AT3G16800.6 36.680 259 114 14 69 282 1 254 8.35e-28 108
Msa1325800 AT1G72770.4 34.457 267 117 8 66 279 238 499 1.06e-27 112
Msa1325800 AT1G72770.1 34.457 267 117 8 66 279 238 499 1.06e-27 112
Msa1325800 AT1G72770.3 34.457 267 117 8 66 279 238 499 1.06e-27 112
Msa1325800 AT3G17250.1 31.148 244 136 8 64 281 156 393 1.57e-27 110
Msa1325800 AT4G26080.1 36.078 255 116 11 66 279 172 420 1.97e-27 110
Msa1325800 AT5G57050.2 36.364 253 119 10 66 279 120 369 2.63e-27 109
Msa1325800 AT3G16800.2 35.849 265 120 14 63 282 90 349 2.76e-27 108
Msa1325800 AT3G16800.1 35.849 265 120 14 63 282 90 349 2.76e-27 108
Msa1325800 AT3G16800.3 35.849 265 120 14 63 282 90 349 2.76e-27 108
Msa1325800 AT5G57050.1 36.364 253 119 10 66 279 160 409 4.60e-27 109
Msa1325800 AT2G40860.4 30.342 234 147 6 59 279 318 548 8.78e-25 103
Msa1325800 AT2G40860.1 30.894 246 154 5 47 279 404 646 1.10e-24 103
Msa1325800 AT1G17550.1 32.959 267 122 9 66 279 237 499 3.08e-24 102
Msa1325800 AT1G17550.2 32.959 267 122 9 66 279 237 499 3.08e-24 102
Msa1325800 AT1G09160.2 33.333 234 130 10 66 279 68 295 1.12e-23 100
Msa1325800 AT1G09160.1 33.333 234 130 10 66 279 68 295 1.12e-23 100
Msa1325800 AT1G18030.1 29.151 271 154 10 44 281 83 348 5.99e-23 97.1
Msa1325800 AT2G40860.2 29.362 235 150 6 47 268 404 635 9.01e-22 95.5
Msa1325800 AT4G08260.1 31.092 238 126 7 48 279 1 206 1.51e-21 90.5
Msa1325800 AT5G27930.1 34.749 259 120 15 69 279 95 352 1.61e-21 93.2
Msa1325800 AT5G27930.2 34.749 259 120 15 69 279 95 352 1.61e-21 93.2
Msa1325800 AT3G05640.2 33.852 257 125 13 69 282 96 350 1.82e-21 92.8
Msa1325800 AT3G05640.1 33.852 257 125 13 69 282 96 350 1.82e-21 92.8
Msa1325800 AT3G05640.3 33.852 257 125 13 69 282 96 350 1.82e-21 92.8
Msa1325800 AT4G27800.1 29.900 301 143 12 33 278 58 345 3.11e-21 92.8
Msa1325800 AT3G51370.1 29.010 293 146 11 31 269 40 324 1.35e-20 90.9
Msa1325800 AT3G51370.3 29.010 293 146 11 31 269 40 324 1.35e-20 90.9
Msa1325800 AT1G47380.1 28.947 266 148 12 37 279 44 291 4.75e-20 89.7
Msa1325800 AT5G02760.1 29.167 288 151 11 10 250 12 293 5.13e-20 89.0
Msa1325800 AT4G38520.2 26.498 317 164 10 31 281 42 355 1.89e-19 87.8
Msa1325800 AT4G38520.1 26.498 317 164 10 31 281 42 355 1.89e-19 87.8
Msa1325800 AT4G31860.1 34.463 177 88 7 126 281 160 329 2.45e-19 87.0
Msa1325800 AT1G18030.2 30.942 223 121 8 44 238 83 300 4.81e-19 85.5
Msa1325800 AT1G68410.4 28.125 256 153 11 49 279 51 300 5.86e-19 86.7
Msa1325800 AT1G68410.3 28.125 256 153 11 49 279 51 300 5.86e-19 86.7
Msa1325800 AT1G68410.1 28.125 256 153 11 49 279 51 300 5.86e-19 86.7
Msa1325800 AT1G68410.2 28.125 256 153 11 49 279 51 300 5.86e-19 86.7
Msa1325800 AT2G25070.1 24.913 289 143 8 61 281 47 329 2.02e-18 84.3
Msa1325800 AT2G25070.2 24.913 289 143 8 61 281 47 329 2.02e-18 84.3
Msa1325800 AT3G12620.2 30.000 320 143 15 32 279 45 355 2.71e-18 84.3
Msa1325800 AT3G12620.1 30.000 320 143 15 32 279 45 355 2.71e-18 84.3
Msa1325800 AT3G55050.1 27.358 318 160 10 32 282 46 359 3.56e-18 84.0
Msa1325800 AT3G55050.2 27.358 318 160 10 32 282 46 359 3.56e-18 84.0
Msa1325800 AT2G20050.2 27.559 254 143 9 66 279 143 395 8.07e-18 84.0
Msa1325800 AT2G20050.1 27.559 254 143 9 66 279 143 395 8.30e-18 84.0
Msa1325800 AT4G31860.3 40.000 135 62 6 126 248 160 287 2.89e-17 80.5
Msa1325800 AT1G79630.6 36.709 158 83 6 125 266 111 267 4.70e-17 80.9
Msa1325800 AT1G79630.3 36.709 158 83 6 125 266 111 267 4.70e-17 80.9
Msa1325800 AT1G79630.4 36.709 158 83 6 125 266 111 267 4.70e-17 80.9
Msa1325800 AT1G79630.5 36.709 158 83 6 125 266 111 267 4.70e-17 80.9
Msa1325800 AT1G79630.2 36.709 158 83 6 125 266 151 307 4.71e-17 80.9
Msa1325800 AT1G79630.7 36.709 158 83 6 125 266 151 307 4.71e-17 80.9
Msa1325800 AT1G79630.1 36.943 157 82 6 126 266 222 377 6.87e-17 80.9
Msa1325800 AT1G79630.8 36.943 157 82 6 126 266 218 373 7.16e-17 80.9
Msa1325800 AT5G06750.3 27.037 270 137 11 28 248 48 306 1.01e-16 79.7
Msa1325800 AT5G06750.1 27.037 270 137 11 28 248 48 306 1.01e-16 79.7
Msa1325800 AT3G02750.2 34.184 196 111 8 88 266 177 371 1.29e-16 80.1
Msa1325800 AT3G02750.1 34.184 196 111 8 88 266 177 371 1.29e-16 80.1
Msa1325800 AT5G01700.1 35.450 189 105 8 93 265 78 265 1.47e-16 79.0
Msa1325800 AT5G66080.1 28.082 292 149 11 32 269 43 327 2.15e-16 79.0
Msa1325800 AT5G01700.2 35.450 189 105 8 93 265 127 314 3.98e-16 78.2
Msa1325800 AT4G03415.1 41.007 139 65 6 126 248 195 332 7.23e-16 77.8
Msa1325800 AT4G03415.3 41.007 139 65 6 126 248 195 332 7.23e-16 77.8
Msa1325800 AT4G03415.2 41.007 139 65 6 126 248 195 332 7.23e-16 77.8
Msa1325800 AT4G33920.1 27.097 310 164 12 7 266 5 302 8.29e-16 77.0
Msa1325800 AT4G32950.1 31.765 255 133 12 63 282 69 317 1.07e-15 76.3
Msa1325800 AT1G16220.1 34.857 175 92 7 126 279 199 372 1.61e-15 76.6
Msa1325800 AT3G27140.1 29.778 225 100 6 61 279 14 186 1.94e-15 74.3
Msa1325800 AT5G26010.1 31.818 220 104 10 66 248 73 283 1.49e-14 73.2
Msa1325800 AT5G36250.1 33.516 182 101 8 103 266 183 362 3.46e-14 72.8
Msa1325800 AT3G17090.2 25.379 264 148 9 29 250 51 307 3.54e-14 72.0
Msa1325800 AT4G31860.2 39.837 123 55 6 126 236 160 275 8.22e-14 70.5
Msa1325800 AT3G51370.2 29.907 214 99 7 102 269 31 239 8.56e-14 70.5
Msa1325800 AT3G06270.1 23.828 256 154 8 66 281 88 342 1.56e-13 70.1
Msa1325800 AT5G19280.1 27.881 269 159 12 41 281 316 577 1.72e-13 70.9
Msa1325800 AT1G03590.1 37.410 139 70 6 126 248 185 322 2.10e-13 70.5
Msa1325800 AT5G59220.2 36.242 149 77 5 69 202 151 296 2.45e-13 69.3
Msa1325800 AT5G66080.2 29.907 214 99 7 102 269 15 223 1.69e-12 66.6
Msa1325800 AT5G19280.2 26.882 279 159 13 41 281 316 587 1.37e-11 65.1
Msa1325800 AT4G33920.2 29.327 208 101 7 102 266 15 219 1.41e-11 64.3
Msa1325800 AT2G05050.1 37.500 96 54 1 190 279 92 187 1.72e-11 62.4
Msa1325800 AT5G27930.3 33.493 209 97 13 68 235 94 301 2.95e-11 63.5

Find 83 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTAATGTAGCCTGATTTC+TGG 0.158630 8_4:+38474444 Msa1325800:intron
TGCTTTCTGTTGTTGGTTCA+TGG 0.226794 8_4:-38474667 None:intergenic
TATTAGCCAGTGACGGATTA+TGG 0.240034 8_4:+38475261 Msa1325800:CDS
ATATCACTGCTTTCTGTTGT+TGG 0.245782 8_4:-38474674 None:intergenic
TTTAGAGAAATCAGGTGAAT+TGG 0.295823 8_4:+38474520 Msa1325800:CDS
GTTGGTGCAGGAGAAAGTTT+TGG 0.308161 8_4:+38473711 Msa1325800:intron
AAGCTAGTGGTGGCCAATAT+CGG 0.310892 8_4:+38474590 Msa1325800:CDS
CTTGTGGAGGATTTCTTTGC+CGG 0.314422 8_4:-38472405 None:intergenic
TGCCGGTCATTGTTGAGTTC+TGG 0.327172 8_4:-38472388 None:intergenic
CTCTTTCCTTTCCCAGAGTC+CGG 0.338408 8_4:-38473745 None:intergenic
GGTTTATTTGCAATATTTGA+TGG 0.353198 8_4:+38473880 Msa1325800:CDS
TGGCCAATATCGGGGATTCT+CGG 0.361781 8_4:+38474600 Msa1325800:CDS
CTGAAGTCATGTGTACCTTT+AGG 0.369744 8_4:-38475190 None:intergenic
TTACTGAAGAAGCGCTTAAC+AGG 0.372627 8_4:+38475510 Msa1325800:CDS
AGCCCGAGAATCCCCGATAT+TGG 0.376877 8_4:-38474603 None:intergenic
AGCTAGTGGTGGCCAATATC+GGG 0.378723 8_4:+38474591 Msa1325800:CDS
TTAGAGAAATCAGGTGAATT+GGG 0.378847 8_4:+38474521 Msa1325800:CDS
GGGGATGTTCCACGGGTTGA+TGG 0.383140 8_4:+38475131 Msa1325800:intron
CATGGCTTTCACTTGGTAAA+GGG 0.396679 8_4:+38473790 Msa1325800:CDS
GGACCATCTGATCCGGACTC+TGG 0.401326 8_4:+38473733 Msa1325800:CDS
TTAGCAGTGTCAAGGGCATT+TGG 0.410576 8_4:+38475158 Msa1325800:CDS
CATGTTGATGACAACGAGTT+GGG 0.413559 8_4:+38473859 Msa1325800:CDS
TGCATTCATACAAATTACTT+TGG 0.422352 8_4:-38475776 None:intergenic
ATGCAATAAATATTTAGTAT+TGG 0.427129 8_4:+38475743 Msa1325800:three_prime_UTR
GGCCAATATCGGGGATTCTC+GGG 0.445298 8_4:+38474601 Msa1325800:CDS
TCATGGCTTTCACTTGGTAA+AGG 0.452390 8_4:+38473789 Msa1325800:CDS
GCAGGTTCAGTCCAGAAATC+AGG 0.467118 8_4:-38474455 None:intergenic
AATAAATATTTAGTATTGGA+TGG 0.467470 8_4:+38475747 Msa1325800:three_prime_UTR
TGCTTTCATTCAAACCAACT+TGG 0.468409 8_4:+38475697 Msa1325800:three_prime_UTR
GTTTGATAATATCTTAAAAG+AGG 0.468636 8_4:+38473945 Msa1325800:CDS
CGGGCTGTCTTATCTGAGAA+TGG 0.477872 8_4:+38474620 Msa1325800:CDS
TACCTTCCATAATCCGTCAC+TGG 0.483949 8_4:-38475267 None:intergenic
CGATTCAGGGGATGTTCCAC+GGG 0.498943 8_4:+38475124 Msa1325800:intron
TCGATTCAGGGGATGTTCCA+CGG 0.499734 8_4:+38475123 Msa1325800:intron
AGATGAGAACGCAAGTAATC+CGG 0.512819 8_4:-38473922 None:intergenic
TCTGGAAGGGTGTCTGTAAG+AGG 0.519312 8_4:-38472370 None:intergenic
ATTTCTTCTCTCATACAACT+TGG 0.520072 8_4:+38472315 Msa1325800:five_prime_UTR
AAATCAGGTGAATTGGGTAA+AGG 0.521064 8_4:+38474527 Msa1325800:CDS
GGATGCTATCAAAGATATCA+AGG 0.533539 8_4:+38475463 Msa1325800:CDS
AGAAATCCTCCACAAGATGA+AGG 0.537571 8_4:+38472412 Msa1325800:CDS
GATGATATACCTTCATCTTG+TGG 0.538998 8_4:-38472421 None:intergenic
TTCCAGAACTCAACAATGAC+CGG 0.539258 8_4:+38472386 Msa1325800:exon
GCATGTTGATGACAACGAGT+TGG 0.541511 8_4:+38473858 Msa1325800:CDS
TTCGTTATATTAGCCAGTGA+CGG 0.544773 8_4:+38475254 Msa1325800:CDS
TGAGCAGAATAACACCTCAA+TGG 0.546519 8_4:-38475645 None:intergenic
TTCCCAGAGTCCGGATCAGA+TGG 0.546662 8_4:-38473736 None:intergenic
AAGTTGGTTTGAATGAAAGC+AGG 0.549427 8_4:-38475695 None:intergenic
TCTGATCCGGACTCTGGGAA+AGG 0.550040 8_4:+38473739 Msa1325800:CDS
GTCATTGTTGAGTTCTGGAA+GGG 0.560441 8_4:-38472383 None:intergenic
ATGTCAAAGCATGTAACTCA+TGG 0.561385 8_4:+38473772 Msa1325800:CDS
TGTAACTCATGGCTTTCACT+TGG 0.561706 8_4:+38473783 Msa1325800:CDS
ATGGACAGTTAGCAGTGTCA+AGG 0.584292 8_4:+38475150 Msa1325800:CDS
GCTCTCTTCACACTATCCGC+AGG 0.588529 8_4:-38474473 None:intergenic
GGTCATTGTTGAGTTCTGGA+AGG 0.592802 8_4:-38472384 None:intergenic
AGCAACCACGTAGTCTTCCA+TGG 0.592925 8_4:-38473827 None:intergenic
ACATCTCTTGCGTTGTTGTG+AGG 0.595080 8_4:+38475546 Msa1325800:CDS
GGGTCAATCAAATCATGCCA+TGG 0.600430 8_4:+38473810 Msa1325800:CDS
GGTGTCTGTAAGAGGTGAAG+AGG 0.602162 8_4:-38472362 None:intergenic
ATTTGGCGACAAAAGCCTAA+AGG 0.604211 8_4:+38475175 Msa1325800:CDS
TGGACAGTTAGCAGTGTCAA+GGG 0.607927 8_4:+38475151 Msa1325800:CDS
CATGTCAAATCAAGAAGCAG+TGG 0.611552 8_4:+38475442 Msa1325800:CDS
AGCCAGTGACGGATTATGGA+AGG 0.612138 8_4:+38475265 Msa1325800:CDS
TCAGGTGAATTGGGTAAAGG+AGG 0.612943 8_4:+38474530 Msa1325800:CDS
AAACAACACAGTATCCAAGT+TGG 0.613452 8_4:-38475711 None:intergenic
TCACGCAGGTCATACTGTAC+CGG 0.619609 8_4:+38473903 Msa1325800:CDS
TCAAAGATATCAAGGATGCT+CGG 0.621211 8_4:+38475471 Msa1325800:CDS
TTCAGTAAGATGCTTTGCTG+CGG 0.623020 8_4:-38475495 None:intergenic
GCAATATTTGATGGTCACGC+AGG 0.624491 8_4:+38473889 Msa1325800:CDS
GATATACCTTCATCTTGTGG+AGG 0.629255 8_4:-38472418 None:intergenic
ATCATCTTCACTGTCACATG+CGG 0.637387 8_4:-38475215 None:intergenic
CTGAAGAAGCGCTTAACAGG+AGG 0.643281 8_4:+38475513 Msa1325800:CDS
GCTAGTGGTGGCCAATATCG+GGG 0.654265 8_4:+38474592 Msa1325800:CDS
AATTAATTGTCAGAAGCTAG+TGG 0.661230 8_4:+38474577 Msa1325800:CDS
ATAAGTGTTGATATCCATTG+AGG 0.661954 8_4:+38475631 Msa1325800:three_prime_UTR
TTGTCTTAGAATAGATGATG+AGG 0.666127 8_4:-38472274 None:intergenic
GCTAACTGTCCATCAACCCG+TGG 0.668653 8_4:-38475140 None:intergenic
GGTTCATGGTCAACCGACAG+TGG 0.669690 8_4:-38474653 None:intergenic
TGATTTCTGGACTGAACCTG+CGG 0.674056 8_4:+38474457 Msa1325800:CDS
TGGTGTTGCTATACCACTGT+CGG 0.682597 8_4:+38474640 Msa1325800:CDS
TAATTGTCAGAAGCTAGTGG+TGG 0.692489 8_4:+38474580 Msa1325800:CDS
TCATGCCATGGAAGACTACG+TGG 0.697697 8_4:+38473822 Msa1325800:CDS
GTGAAGATGATAGATGACGG+TGG 0.723275 8_4:+38475227 Msa1325800:CDS
ACAGTGAAGATGATAGATGA+CGG 0.770678 8_4:+38475224 Msa1325800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCAAATATTATAGTTAAT+TGG + chr8_4:38472585-38472604 Msa1325800:intron 10.0%
!! TTGAAATCAAATCAAATATA+AGG - chr8_4:38472507-38472526 None:intergenic 15.0%
!!! CTATTGAATTATTTTTCTTT+CGG - chr8_4:38473355-38473374 None:intergenic 15.0%
!! AAAGAAAAATAATTCAATAG+AGG + chr8_4:38473355-38473374 Msa1325800:intron 15.0%
!!! ATAATTTTTCAGTTTTTGTT+TGG - chr8_4:38474229-38474248 None:intergenic 15.0%
!! AGGTAAAATATTTGATTAAA+TGG + chr8_4:38475285-38475304 Msa1325800:intron 15.0%
!! GGTAAAATATTTGATTAAAT+GGG + chr8_4:38475286-38475305 Msa1325800:intron 15.0%
!!! ATATTCTCATTTGTTATTAT+AGG + chr8_4:38475418-38475437 Msa1325800:intron 15.0%
!! ATGCAATAAATATTTAGTAT+TGG + chr8_4:38475743-38475762 Msa1325800:three_prime_UTR 15.0%
!!! AATAAATATTTAGTATTGGA+TGG + chr8_4:38475747-38475766 Msa1325800:three_prime_UTR 15.0%
!! AGCTAATTGAAAATTAAAGA+TGG - chr8_4:38472530-38472549 None:intergenic 20.0%
!! TTTCAAATTATTAGTTGTGT+CGG + chr8_4:38473076-38473095 Msa1325800:intron 20.0%
!! AAATGTTTCTTATATTAAGG+AGG - chr8_4:38473174-38473193 None:intergenic 20.0%
!! GTGAAATGTTTCTTATATTA+AGG - chr8_4:38473177-38473196 None:intergenic 20.0%
!!! TTTTTAAGTTCATTAATGAC+CGG + chr8_4:38473197-38473216 Msa1325800:intron 20.0%
!!! ATTTTTTCTTATGTTTCAAC+TGG + chr8_4:38473683-38473702 Msa1325800:intron 20.0%
!!! GTTTGATAATATCTTAAAAG+AGG + chr8_4:38473945-38473964 Msa1325800:CDS 20.0%
!! ATTATCAATTTCAATTCAAG+TGG + chr8_4:38474244-38474263 Msa1325800:intron 20.0%
!!! ATTTATCTTTTTTCTACACT+TGG + chr8_4:38474333-38474352 Msa1325800:intron 20.0%
! TATGAAGAGAAAAAAGCAAA+GGG - chr8_4:38472454-38472473 None:intergenic 25.0%
! ATATGAAGAGAAAAAAGCAA+AGG - chr8_4:38472455-38472474 None:intergenic 25.0%
! TAGTTAATTGGTTAATTAGC+AGG + chr8_4:38472597-38472616 Msa1325800:intron 25.0%
! TTTACCAGTTAATTGTTATG+TGG + chr8_4:38472631-38472650 Msa1325800:intron 25.0%
!!! TTTTATTTTGCTTTCGTAAC+CGG + chr8_4:38472793-38472812 Msa1325800:intron 25.0%
! GCAATATCAATCTATCAAAT+TGG + chr8_4:38472927-38472946 Msa1325800:intron 25.0%
! ATATCAATCTATCAAATTGG+TGG + chr8_4:38472930-38472949 Msa1325800:intron 25.0%
! ATAATATAGATCAGATACAC+CGG - chr8_4:38473219-38473238 None:intergenic 25.0%
! TATAATCTAACAGTTGTAAC+AGG + chr8_4:38473468-38473487 Msa1325800:intron 25.0%
! AATATCTTACTAGTTACCAT+TGG + chr8_4:38473584-38473603 Msa1325800:intron 25.0%
! GGTTTATTTGCAATATTTGA+TGG + chr8_4:38473880-38473899 Msa1325800:CDS 25.0%
! ATATCTTAAAAGAGGTAATG+AGG + chr8_4:38473953-38473972 Msa1325800:intron 25.0%
! ACTCTAAGTAATCACAAAAA+CGG - chr8_4:38473983-38474002 None:intergenic 25.0%
!!! TCTACTATTTTAGAGAAATC+AGG + chr8_4:38474512-38474531 Msa1325800:CDS 25.0%
! AAAACATGATATACTATACC+TGG - chr8_4:38474731-38474750 None:intergenic 25.0%
! TCTATATGTTATTCAGTATC+TGG + chr8_4:38474885-38474904 Msa1325800:intron 25.0%
! AAGGATACAGTTTATATCTA+AGG + chr8_4:38475085-38475104 Msa1325800:intron 25.0%
!!! ACATATTTTGTTATCGATTC+AGG + chr8_4:38475110-38475129 Msa1325800:intron 25.0%
!!! CATATTTTGTTATCGATTCA+GGG + chr8_4:38475111-38475130 Msa1325800:intron 25.0%
!!! ATATTTTGTTATCGATTCAG+GGG + chr8_4:38475112-38475131 Msa1325800:intron 25.0%
! TGCATTCATACAAATTACTT+TGG - chr8_4:38475779-38475798 None:intergenic 25.0%
TTGTCTTAGAATAGATGATG+AGG - chr8_4:38472277-38472296 None:intergenic 30.0%
ATTTCTTCTCTCATACAACT+TGG + chr8_4:38472315-38472334 Msa1325800:five_prime_UTR 30.0%
TAATTGGTTAATTAGCAGGA+TGG + chr8_4:38472601-38472620 Msa1325800:intron 30.0%
!! CATTTAATTCTGAAGTTTGC+TGG - chr8_4:38472657-38472676 None:intergenic 30.0%
TAAATGTTAGCCCAATATCA+CGG + chr8_4:38472671-38472690 Msa1325800:intron 30.0%
! ATAATTGTCTTATTCACCAG+AGG + chr8_4:38472848-38472867 Msa1325800:intron 30.0%
TTCAGTGTAATTACAAGTGT+CGG - chr8_4:38473045-38473064 None:intergenic 30.0%
! ATTTTCTCAAGATTTGCCAA+TGG - chr8_4:38473603-38473622 None:intergenic 30.0%
GTGCAATTTATCTATTCATG+TGG - chr8_4:38473627-38473646 None:intergenic 30.0%
CTCTAAGTAATCACAAAAAC+GGG - chr8_4:38473982-38474001 None:intergenic 30.0%
GTGATTTCTCATAATGCATA+GGG - chr8_4:38474099-38474118 None:intergenic 30.0%
! CATAGATTAAGCCTATGTTT+TGG - chr8_4:38474157-38474176 None:intergenic 30.0%
!! TTTCAGTTTTTGTTTGGAAC+CGG - chr8_4:38474223-38474242 None:intergenic 30.0%
AAACTCTTGAAACATGCAAA+AGG - chr8_4:38474305-38474324 None:intergenic 30.0%
!!! TTTTTTCTACACTTGGTGAA+TGG + chr8_4:38474340-38474359 Msa1325800:intron 30.0%
!! GTATTTTTACCGTATTCATC+TGG + chr8_4:38474378-38474397 Msa1325800:intron 30.0%
TTTAGAGAAATCAGGTGAAT+TGG + chr8_4:38474520-38474539 Msa1325800:CDS 30.0%
TTAGAGAAATCAGGTGAATT+GGG + chr8_4:38474521-38474540 Msa1325800:CDS 30.0%
AATTAATTGTCAGAAGCTAG+TGG + chr8_4:38474577-38474596 Msa1325800:CDS 30.0%
AGCAGTGATATAAAAAACAG+AGG + chr8_4:38474686-38474705 Msa1325800:CDS 30.0%
AGTGATATAAAAAACAGAGG+TGG + chr8_4:38474689-38474708 Msa1325800:CDS 30.0%
CAATGATGCATAGATATGAA+AGG - chr8_4:38474837-38474856 None:intergenic 30.0%
GCATCAATACGTGATATTTA+AGG + chr8_4:38474918-38474937 Msa1325800:intron 30.0%
CACTGAATAATTTGAGAAGA+TGG - chr8_4:38474990-38475009 None:intergenic 30.0%
ACCAAACATACATGTTTGAT+TGG + chr8_4:38475368-38475387 Msa1325800:intron 30.0%
TCCAATCAAACATGTATGTT+TGG - chr8_4:38475372-38475391 None:intergenic 30.0%
!! ATAAGTGTTGATATCCATTG+AGG + chr8_4:38475631-38475650 Msa1325800:three_prime_UTR 30.0%
GAAGAGGCGAAAAAATAATG+AGG - chr8_4:38472349-38472368 None:intergenic 35.0%
GATGATATACCTTCATCTTG+TGG - chr8_4:38472424-38472443 None:intergenic 35.0%
AAATGTTAGCCCAATATCAC+GGG + chr8_4:38472672-38472691 Msa1325800:intron 35.0%
AATCACTTTCCCGTGATATT+GGG - chr8_4:38472684-38472703 None:intergenic 35.0%
CAATGATAGCAGTATTGTAC+TGG + chr8_4:38472712-38472731 Msa1325800:intron 35.0%
TATAGAGATGCTATGAAGCA+CGG + chr8_4:38472956-38472975 Msa1325800:intron 35.0%
AGAGATGTCAAAAAGCAAGT+TGG + chr8_4:38473137-38473156 Msa1325800:intron 35.0%
TGACATATGTATGTGAGCTA+TGG + chr8_4:38473381-38473400 Msa1325800:intron 35.0%
CTAAATGCATCAGATACTTG+AGG - chr8_4:38473536-38473555 None:intergenic 35.0%
TGATGCATTTAGCCACTTAT+TGG + chr8_4:38473544-38473563 Msa1325800:intron 35.0%
GATGCATTTAGCCACTTATT+GGG + chr8_4:38473545-38473564 Msa1325800:intron 35.0%
ATGTGTATCTTCCCAATAAG+TGG - chr8_4:38473559-38473578 None:intergenic 35.0%
ATGTTTCAACTGGAGAATGT+TGG + chr8_4:38473693-38473712 Msa1325800:intron 35.0%
ATGTCAAAGCATGTAACTCA+TGG + chr8_4:38473772-38473791 Msa1325800:CDS 35.0%
GGTGATTTCTCATAATGCAT+AGG - chr8_4:38474100-38474119 None:intergenic 35.0%
AAATCACCACCAATTTGCAT+TGG + chr8_4:38474071-38474090 Msa1325800:intron 35.0%
AATCACCACCAATTTGCATT+GGG + chr8_4:38474072-38474091 Msa1325800:intron 35.0%
! TTTTGGCACACTTATGCATA+GGG - chr8_4:38474140-38474159 None:intergenic 35.0%
!!! CTTGGTGAATGGTTTTGATT+TGG + chr8_4:38474351-38474370 Msa1325800:intron 35.0%
AAATCAGGTGAATTGGGTAA+AGG + chr8_4:38474527-38474546 Msa1325800:CDS 35.0%
ATATCACTGCTTTCTGTTGT+TGG - chr8_4:38474677-38474696 None:intergenic 35.0%
!! GGTTTTGTATCTAACTTTCC+AGG + chr8_4:38474710-38474729 Msa1325800:CDS 35.0%
! GAATAGCTGTATTGATGTAC+TGG + chr8_4:38474808-38474827 Msa1325800:intron 35.0%
ATTAAATGTCTCTACACCGA+AGG - chr8_4:38474962-38474981 None:intergenic 35.0%
GTGTCTATTTGTCACTATCA+TGG + chr8_4:38475007-38475026 Msa1325800:intron 35.0%
GTTCCTAAAATATTTGCTCG+TGG + chr8_4:38475060-38475079 Msa1325800:intron 35.0%
! TAGCCACGAGCAAATATTTT+AGG - chr8_4:38475066-38475085 None:intergenic 35.0%
AAAATATTTGCTCGTGGCTA+AGG + chr8_4:38475066-38475085 Msa1325800:intron 35.0%
ACAGTGAAGATGATAGATGA+CGG + chr8_4:38475224-38475243 Msa1325800:CDS 35.0%
TTCGTTATATTAGCCAGTGA+CGG + chr8_4:38475254-38475273 Msa1325800:CDS 35.0%
GGATGCTATCAAAGATATCA+AGG + chr8_4:38475463-38475482 Msa1325800:CDS 35.0%
TCAAAGATATCAAGGATGCT+CGG + chr8_4:38475471-38475490 Msa1325800:CDS 35.0%
!! AAGTTGGTTTGAATGAAAGC+AGG - chr8_4:38475698-38475717 None:intergenic 35.0%
TGCTTTCATTCAAACCAACT+TGG + chr8_4:38475697-38475716 Msa1325800:three_prime_UTR 35.0%
AAACAACACAGTATCCAAGT+TGG - chr8_4:38475714-38475733 None:intergenic 35.0%
!! GTCATTGTTGAGTTCTGGAA+GGG - chr8_4:38472386-38472405 None:intergenic 40.0%
TTCCAGAACTCAACAATGAC+CGG + chr8_4:38472386-38472405 Msa1325800:exon 40.0%
AGAAATCCTCCACAAGATGA+AGG + chr8_4:38472412-38472431 Msa1325800:CDS 40.0%
GATATACCTTCATCTTGTGG+AGG - chr8_4:38472421-38472440 None:intergenic 40.0%
CTGGCCACATAACAATTAAC+TGG - chr8_4:38472638-38472657 None:intergenic 40.0%
GAATCACTTTCCCGTGATAT+TGG - chr8_4:38472685-38472704 None:intergenic 40.0%
TATCACGGGAAAGTGATTCA+TGG + chr8_4:38472686-38472705 Msa1325800:intron 40.0%
ATCACGGGAAAGTGATTCAT+GGG + chr8_4:38472687-38472706 Msa1325800:intron 40.0%
ATCATACTAACCAATGCACC+CGG - chr8_4:38472823-38472842 None:intergenic 40.0%
TCTACTATGATGCACGGATA+CGG - chr8_4:38473120-38473139 None:intergenic 40.0%
GACATCTCTACTATGATGCA+CGG - chr8_4:38473126-38473145 None:intergenic 40.0%
TTGAGAAAAGTTACTCGCTC+AGG - chr8_4:38473299-38473318 None:intergenic 40.0%
GAAAATCAGACGAGTGTGAA+AGG + chr8_4:38473321-38473340 Msa1325800:intron 40.0%
! TTGGTGCAGGAGAAAGTTTT+GGG + chr8_4:38473712-38473731 Msa1325800:intron 40.0%
TGTAACTCATGGCTTTCACT+TGG + chr8_4:38473783-38473802 Msa1325800:CDS 40.0%
TCATGGCTTTCACTTGGTAA+AGG + chr8_4:38473789-38473808 Msa1325800:CDS 40.0%
CATGGCTTTCACTTGGTAAA+GGG + chr8_4:38473790-38473809 Msa1325800:CDS 40.0%
!! CATGTTGATGACAACGAGTT+GGG + chr8_4:38473859-38473878 Msa1325800:CDS 40.0%
AGATGAGAACGCAAGTAATC+CGG - chr8_4:38473925-38473944 None:intergenic 40.0%
TGACGTGTGTTTGATGTGAA+TGG - chr8_4:38474023-38474042 None:intergenic 40.0%
CATAGGGACACAATGCAAAT+TGG - chr8_4:38474083-38474102 None:intergenic 40.0%
!! GTTTTGGCACACTTATGCAT+AGG - chr8_4:38474141-38474160 None:intergenic 40.0%
GCATAAGTGTGCCAAAACAT+AGG + chr8_4:38474143-38474162 Msa1325800:intron 40.0%
CCAACATCTCCAGATGAATA+CGG - chr8_4:38474390-38474409 None:intergenic 40.0%
GTCTAATGTAGCCTGATTTC+TGG + chr8_4:38474444-38474463 Msa1325800:intron 40.0%
TAATTGTCAGAAGCTAGTGG+TGG + chr8_4:38474580-38474599 Msa1325800:CDS 40.0%
!! TGCTTTCTGTTGTTGGTTCA+TGG - chr8_4:38474670-38474689 None:intergenic 40.0%
ATTTGGCGACAAAAGCCTAA+AGG + chr8_4:38475175-38475194 Msa1325800:CDS 40.0%
CTGAAGTCATGTGTACCTTT+AGG - chr8_4:38475193-38475212 None:intergenic 40.0%
ATCATCTTCACTGTCACATG+CGG - chr8_4:38475218-38475237 None:intergenic 40.0%
!! TATTAGCCAGTGACGGATTA+TGG + chr8_4:38475261-38475280 Msa1325800:CDS 40.0%
ATTCCTTTGCACTCACCTTA+TGG + chr8_4:38475330-38475349 Msa1325800:intron 40.0%
TTCCTTTGCACTCACCTTAT+GGG + chr8_4:38475331-38475350 Msa1325800:intron 40.0%
TAGAAGCAAAACTGCCCATA+AGG - chr8_4:38475348-38475367 None:intergenic 40.0%
CATGTCAAATCAAGAAGCAG+TGG + chr8_4:38475442-38475461 Msa1325800:CDS 40.0%
TTCAGTAAGATGCTTTGCTG+CGG - chr8_4:38475498-38475517 None:intergenic 40.0%
!! TTACTGAAGAAGCGCTTAAC+AGG + chr8_4:38475510-38475529 Msa1325800:CDS 40.0%
TGAGCAGAATAACACCTCAA+TGG - chr8_4:38475648-38475667 None:intergenic 40.0%
!! GGTCATTGTTGAGTTCTGGA+AGG - chr8_4:38472387-38472406 None:intergenic 45.0%
CTTGTGGAGGATTTCTTTGC+CGG - chr8_4:38472408-38472427 None:intergenic 45.0%
TCATACTAACCAATGCACCC+GGG - chr8_4:38472822-38472841 None:intergenic 45.0%
! ATTCACCAGAGGCATGTGAA+AGG + chr8_4:38472859-38472878 Msa1325800:intron 45.0%
CAGCTAGCTAAGCTAACAAC+GGG - chr8_4:38472908-38472927 None:intergenic 45.0%
GCAGCTAGCTAAGCTAACAA+CGG - chr8_4:38472909-38472928 None:intergenic 45.0%
! GTTGGTGCAGGAGAAAGTTT+TGG + chr8_4:38473711-38473730 Msa1325800:intron 45.0%
!!! AGTTTTGGGACCATCTGATC+CGG + chr8_4:38473726-38473745 Msa1325800:CDS 45.0%
GGGTCAATCAAATCATGCCA+TGG + chr8_4:38473810-38473829 Msa1325800:CDS 45.0%
!! GCATGTTGATGACAACGAGT+TGG + chr8_4:38473858-38473877 Msa1325800:CDS 45.0%
! GCAATATTTGATGGTCACGC+AGG + chr8_4:38473889-38473908 Msa1325800:CDS 45.0%
AGGGACACAATGCAAATTGG+TGG - chr8_4:38474080-38474099 None:intergenic 45.0%
CATAGGGACCCAATGCAAAT+TGG - chr8_4:38474124-38474143 None:intergenic 45.0%
!! GTTTTTGTTTGGAACCGGCA+TGG - chr8_4:38474218-38474237 None:intergenic 45.0%
CCGTATTCATCTGGAGATGT+TGG + chr8_4:38474387-38474406 Msa1325800:intron 45.0%
!!! CATCTGGAGATGTTGGCTTT+TGG + chr8_4:38474394-38474413 Msa1325800:intron 45.0%
! TGATTTCTGGACTGAACCTG+CGG + chr8_4:38474457-38474476 Msa1325800:CDS 45.0%
TCAGGTGAATTGGGTAAAGG+AGG + chr8_4:38474530-38474549 Msa1325800:CDS 45.0%
AAGCTAGTGGTGGCCAATAT+CGG + chr8_4:38474590-38474609 Msa1325800:CDS 45.0%
!! TGGTGTTGCTATACCACTGT+CGG + chr8_4:38474640-38474659 Msa1325800:CDS 45.0%
TTGTCACTATCATGGTTGCG+TGG + chr8_4:38475015-38475034 Msa1325800:intron 45.0%
ATGGACAGTTAGCAGTGTCA+AGG + chr8_4:38475150-38475169 Msa1325800:CDS 45.0%
TGGACAGTTAGCAGTGTCAA+GGG + chr8_4:38475151-38475170 Msa1325800:CDS 45.0%
!! TTAGCAGTGTCAAGGGCATT+TGG + chr8_4:38475158-38475177 Msa1325800:CDS 45.0%
! GTGAAGATGATAGATGACGG+TGG + chr8_4:38475227-38475246 Msa1325800:CDS 45.0%
TACCTTCCATAATCCGTCAC+TGG - chr8_4:38475270-38475289 None:intergenic 45.0%
ACATCTCTTGCGTTGTTGTG+AGG + chr8_4:38475546-38475565 Msa1325800:CDS 45.0%
GGTGTCTGTAAGAGGTGAAG+AGG - chr8_4:38472365-38472384 None:intergenic 50.0%
!! TCTGGAAGGGTGTCTGTAAG+AGG - chr8_4:38472373-38472392 None:intergenic 50.0%
TGCCGGTCATTGTTGAGTTC+TGG - chr8_4:38472391-38472410 None:intergenic 50.0%
CATACTAACCAATGCACCCG+GGG - chr8_4:38472821-38472840 None:intergenic 50.0%
AACCACCTTTCACATGCCTC+TGG - chr8_4:38472867-38472886 None:intergenic 50.0%
TATGAAGCACGGACACTCCT+CGG + chr8_4:38472967-38472986 Msa1325800:intron 50.0%
!! TACAAGTGTCGGTGTCGTGT+CGG - chr8_4:38473034-38473053 None:intergenic 50.0%
CAACTGGAGAATGTTGGTGC+AGG + chr8_4:38473699-38473718 Msa1325800:intron 50.0%
CTCTTTCCTTTCCCAGAGTC+CGG - chr8_4:38473748-38473767 None:intergenic 50.0%
TCATGCCATGGAAGACTACG+TGG + chr8_4:38473822-38473841 Msa1325800:CDS 50.0%
AGCAACCACGTAGTCTTCCA+TGG - chr8_4:38473830-38473849 None:intergenic 50.0%
TCACGCAGGTCATACTGTAC+CGG + chr8_4:38473903-38473922 Msa1325800:CDS 50.0%
AGGGACCCAATGCAAATTGG+TGG - chr8_4:38474121-38474140 None:intergenic 50.0%
GGCTAGGGGTAATGCATTTC+AGG - chr8_4:38474197-38474216 None:intergenic 50.0%
GCAGGTTCAGTCCAGAAATC+AGG - chr8_4:38474458-38474477 None:intergenic 50.0%
AGCTAGTGGTGGCCAATATC+GGG + chr8_4:38474591-38474610 Msa1325800:CDS 50.0%
TGGCCAATATCGGGGATTCT+CGG + chr8_4:38474600-38474619 Msa1325800:CDS 50.0%
CGGGCTGTCTTATCTGAGAA+TGG + chr8_4:38474620-38474639 Msa1325800:CDS 50.0%
!! TACACCGAAGGTGTTGGACA+CGG - chr8_4:38474950-38474969 None:intergenic 50.0%
! TGTCTCTACACCGAAGGTGT+TGG - chr8_4:38474956-38474975 None:intergenic 50.0%
TCGATTCAGGGGATGTTCCA+CGG + chr8_4:38475123-38475142 Msa1325800:intron 50.0%
!! AGCCAGTGACGGATTATGGA+AGG + chr8_4:38475265-38475284 Msa1325800:CDS 50.0%
TGCCCATAAGGTGAGTGCAA+AGG - chr8_4:38475336-38475355 None:intergenic 50.0%
!! CTGAAGAAGCGCTTAACAGG+AGG + chr8_4:38475513-38475532 Msa1325800:CDS 50.0%
! CACCAGAGGCATGTGAAAGG+TGG + chr8_4:38472862-38472881 Msa1325800:intron 55.0%
GATTAGGCGTGTCCTGGTGT+TGG + chr8_4:38472989-38473008 Msa1325800:intron 55.0%
CACGACACATGTCCAACACC+AGG - chr8_4:38473004-38473023 None:intergenic 55.0%
TGATGCACGGATACGGACAC+CGG - chr8_4:38473113-38473132 None:intergenic 55.0%
GACCATCTGATCCGGACTCT+GGG + chr8_4:38473734-38473753 Msa1325800:CDS 55.0%
TTCCCAGAGTCCGGATCAGA+TGG - chr8_4:38473739-38473758 None:intergenic 55.0%
! TCTGATCCGGACTCTGGGAA+AGG + chr8_4:38473739-38473758 Msa1325800:CDS 55.0%
TGCATTACCCCTAGCCATGC+CGG + chr8_4:38474201-38474220 Msa1325800:intron 55.0%
! TTTGGAACCGGCATGGCTAG+GGG - chr8_4:38474211-38474230 None:intergenic 55.0%
! GTTTGGAACCGGCATGGCTA+GGG - chr8_4:38474212-38474231 None:intergenic 55.0%
! TGTTTGGAACCGGCATGGCT+AGG - chr8_4:38474213-38474232 None:intergenic 55.0%
GCTCTCTTCACACTATCCGC+AGG - chr8_4:38474476-38474495 None:intergenic 55.0%
GCTAGTGGTGGCCAATATCG+GGG + chr8_4:38474592-38474611 Msa1325800:CDS 55.0%
GGCCAATATCGGGGATTCTC+GGG + chr8_4:38474601-38474620 Msa1325800:CDS 55.0%
AGCCCGAGAATCCCCGATAT+TGG - chr8_4:38474606-38474625 None:intergenic 55.0%
GGTTCATGGTCAACCGACAG+TGG - chr8_4:38474656-38474675 None:intergenic 55.0%
TGTCCCGTGTCCAACACCTT+CGG + chr8_4:38474943-38474962 Msa1325800:intron 55.0%
!! ACACCGAAGGTGTTGGACAC+GGG - chr8_4:38474949-38474968 None:intergenic 55.0%
CGATTCAGGGGATGTTCCAC+GGG + chr8_4:38475124-38475143 Msa1325800:intron 55.0%
GCTAACTGTCCATCAACCCG+TGG - chr8_4:38475143-38475162 None:intergenic 55.0%
TGCTTTCGTAACCGGTGCCC+CGG + chr8_4:38472801-38472820 Msa1325800:intron 60.0%
GCACGGACACTCCTCGGATT+AGG + chr8_4:38472973-38472992 Msa1325800:intron 60.0%
! TCCTCGGATTAGGCGTGTCC+TGG + chr8_4:38472983-38473002 Msa1325800:intron 60.0%
ACCAGGACACGCCTAATCCG+AGG - chr8_4:38472987-38473006 None:intergenic 60.0%
!! TGTCGGTGTCGTGTCGGTGT+CGG - chr8_4:38473028-38473047 None:intergenic 60.0%
! TGTGTCGGCGTCTCAGTGTC+CGG + chr8_4:38473091-38473110 Msa1325800:intron 60.0%
GGACCATCTGATCCGGACTC+TGG + chr8_4:38473733-38473752 Msa1325800:CDS 60.0%
! GGGGATGTTCCACGGGTTGA+TGG + chr8_4:38475131-38475150 Msa1325800:intron 60.0%
GCTTTCGTAACCGGTGCCCC+GGG + chr8_4:38472802-38472821 Msa1325800:intron 65.0%
AACCAATGCACCCGGGGCAC+CGG - chr8_4:38472815-38472834 None:intergenic 65.0%
AACCGGTGCCCCGGGTGCAT+TGG + chr8_4:38472810-38472829 Msa1325800:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 38472253 38475831 38472253 ID=Msa1325800;Name=Msa1325800
chr8_4 mRNA 38472253 38475831 38472253 ID=Msa1325800-mRNA-1;Parent=Msa1325800;Name=Msa1325800-mRNA-1;_AED=0.16;_eAED=0.16;_QI=148|1|1|1|1|1|5|254|282
chr8_4 exon 38472253 38472433 38472253 ID=Msa1325800-mRNA-1:exon:11671;Parent=Msa1325800-mRNA-1
chr8_4 exon 38473721 38473966 38473721 ID=Msa1325800-mRNA-1:exon:11672;Parent=Msa1325800-mRNA-1
chr8_4 exon 38474455 38474731 38474455 ID=Msa1325800-mRNA-1:exon:11673;Parent=Msa1325800-mRNA-1
chr8_4 exon 38475132 38475286 38475132 ID=Msa1325800-mRNA-1:exon:11674;Parent=Msa1325800-mRNA-1
chr8_4 exon 38475440 38475831 38475440 ID=Msa1325800-mRNA-1:exon:11675;Parent=Msa1325800-mRNA-1
chr8_4 five_prime_UTR 38472253 38472400 38472253 ID=Msa1325800-mRNA-1:five_prime_utr;Parent=Msa1325800-mRNA-1
chr8_4 CDS 38472401 38472433 38472401 ID=Msa1325800-mRNA-1:cds;Parent=Msa1325800-mRNA-1
chr8_4 CDS 38473721 38473966 38473721 ID=Msa1325800-mRNA-1:cds;Parent=Msa1325800-mRNA-1
chr8_4 CDS 38474455 38474731 38474455 ID=Msa1325800-mRNA-1:cds;Parent=Msa1325800-mRNA-1
chr8_4 CDS 38475132 38475286 38475132 ID=Msa1325800-mRNA-1:cds;Parent=Msa1325800-mRNA-1
chr8_4 CDS 38475440 38475577 38475440 ID=Msa1325800-mRNA-1:cds;Parent=Msa1325800-mRNA-1
chr8_4 three_prime_UTR 38475578 38475831 38475578 ID=Msa1325800-mRNA-1:three_prime_utr;Parent=Msa1325800-mRNA-1
Gene Sequence

>Msa1325800

ATGACCGGCAAAGAAATCCTCCACAAGATGAAGGAGAAAGTTTTGGGACCATCTGATCCGGACTCTGGGAAAGGAAAGAGCAAGATGTCAAAGCATGTAACTCATGGCTTTCACTTGGTAAAGGGTCAATCAAATCATGCCATGGAAGACTACGTGGTTGCTGAATTCAAGCATGTTGATGACAACGAGTTGGGTTTATTTGCAATATTTGATGGTCACGCAGGTCATACTGTACCGGATTACTTGCGTTCTCATCTGTTTGATAATATCTTAAAAGAGCCTGATTTCTGGACTGAACCTGCGGATAGTGTGAAGAGAGCATATAAAGAAACTGATTCTACTATTTTAGAGAAATCAGGTGAATTGGGTAAAGGAGGTTCAACTGCAGTTACAGCAATACTAATTAATTGTCAGAAGCTAGTGGTGGCCAATATCGGGGATTCTCGGGCTGTCTTATCTGAGAATGGTGTTGCTATACCACTGTCGGTTGACCATGAACCAACAACAGAAAGCAGTGATATAAAAAACAGAGGTGGTTTTGTATCTAACTTTCCAGGGGATGTTCCACGGGTTGATGGACAGTTAGCAGTGTCAAGGGCATTTGGCGACAAAAGCCTAAAGGTACACATGACTTCAGAACCGCATGTGACAGTGAAGATGATAGATGACGGTGGAGAGTTCGTTATATTAGCCAGTGACGGATTATGGAAGGTCATGTCAAATCAAGAAGCAGTGGATGCTATCAAAGATATCAAGGATGCTCGGTCCGCAGCAAAGCATCTTACTGAAGAAGCGCTTAACAGGAGGAGCTCAGATGACATCTCTTGCGTTGTTGTGAGGTTTCACTGA
Protein sequence

>Msa1325800

MTGKEILHKMKEKVLGPSDPDSGKGKSKMSKHVTHGFHLVKGQSNHAMEDYVVAEFKHVDDNELGLFAIFDGHAGHTVPDYLRSHLFDNILKEPDFWTEPADSVKRAYKETDSTILEKSGELGKGGSTAVTAILINCQKLVVANIGDSRAVLSENGVAIPLSVDHEPTTESSDIKNRGGFVSNFPGDVPRVDGQLAVSRAFGDKSLKVHMTSEPHVTVKMIDDGGEFVILASDGLWKVMSNQEAVDAIKDIKDARSAAKHLTEEALNRRSSDDISCVVVRFH*