AlfalfaGEDB | Msa1358710

M. sativa cultivar zhongmu-4 / Msa1358710

KEGG: K02898
GO: GO:0002181 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010467 GO:0015934 GO:0016020 GO:0019538 GO:0022613 GO:0022625 GO:0022626 GO:0031090 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0034645 GO:0042254 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0050896 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0098588 GO:0098805 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF16906.8

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1358710	XP_003611586.2	100.000	146	0	0	1	146	1	146	2.75e-99	291

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1358710	sp\|Q9FJX2\|RL262_ARATH	86.301	146	20	0	1	146	1	146	2.49e-90	261

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1358710	G7K2J3	100.000	146	0	0	1	146	1	146	1.31e-99	291

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001760	Msa1358710	0.848972	4.358011e-60	-8.615850e-47
Msa0027590	Msa1358710	0.863817	1.898053e-64	-8.615850e-47
Msa0075350	Msa1358710	0.819133	1.362762e-52	-8.615850e-47
Msa1351020	Msa1358710	0.827642	1.397624e-54	-8.615850e-47
Msa1358710	Msa1385480	0.846797	1.732874e-59	-8.615850e-47
Msa1358710	Msa1390760	0.827894	1.215773e-54	-8.615850e-47
Msa1358710	Msa1432310	0.825061	5.754303e-54	-8.615850e-47
Msa1358710	Msa1463570	0.827045	1.943003e-54	-8.615850e-47
Msa1358710	Msa1465700	0.831569	1.549718e-55	-8.615850e-47
Msa0602900	Msa1358710	0.816114	6.533141e-52	-8.615850e-47
Msa0605890	Msa1358710	0.816071	6.678627e-52	-8.615850e-47
Msa0619230	Msa1358710	0.829147	6.056297e-55	-8.615850e-47
Msa0643960	Msa1358710	0.817273	3.592110e-52	-8.615850e-47
Msa0694400	Msa1358710	0.866646	2.448383e-65	-8.615850e-47
Msa0704860	Msa1358710	0.833928	4.023034e-56	-8.615850e-47
Msa0708630	Msa1358710	0.859310	4.519253e-63	-8.615850e-47
Msa0128330	Msa1358710	0.830701	2.531777e-55	-8.615850e-47
Msa0132460	Msa1358710	0.860587	1.861729e-63	-8.615850e-47
Msa0134580	Msa1358710	0.816165	6.365988e-52	-8.615850e-47
Msa0167860	Msa1358710	0.818406	1.992660e-52	-8.615850e-47
Msa0168320	Msa1358710	0.815022	1.143551e-51	-8.615850e-47
Msa0174580	Msa1358710	0.808681	2.748409e-50	-8.615850e-47
Msa0182050	Msa1358710	0.862970	3.474817e-64	-8.615850e-47
Msa0182100	Msa1358710	0.823298	1.493314e-53	-8.615850e-47
Msa0191450	Msa1358710	0.803185	3.933582e-49	-8.615850e-47
Msa0191550	Msa1358710	0.813908	2.017353e-51	-8.615850e-47
Msa0192300	Msa1358710	0.818366	2.034993e-52	-8.615850e-47
Msa1235950	Msa1358710	0.836249	1.045165e-56	-8.615850e-47
Msa1235980	Msa1358710	0.839191	1.836237e-57	-8.615850e-47
Msa1237040	Msa1358710	0.820347	7.197060e-53	-8.615850e-47
Msa1316100	Msa1358710	0.827223	1.761847e-54	-8.615850e-47
Msa1317390	Msa1358710	0.845787	3.265850e-59	-8.615850e-47
Msa0369410	Msa1358710	0.816964	4.214131e-52	-8.615850e-47
Msa0371490	Msa1358710	0.816670	4.907160e-52	-8.615850e-47
Msa0377520	Msa1358710	0.831626	1.500679e-55	-8.615850e-47
Msa0426090	Msa1358710	0.840981	6.266374e-58	-8.615850e-47
Msa0426250	Msa1358710	0.816283	5.989629e-52	-8.615850e-47
Msa0472480	Msa1358710	0.844956	5.482968e-59	-8.615850e-47
Msa0473390	Msa1358710	0.800827	1.200612e-48	-8.615850e-47
Msa1040160	Msa1358710	0.825462	4.626703e-54	-8.615850e-47
Msa1041680	Msa1358710	0.815177	1.056479e-51	-8.615850e-47
Msa1083370	Msa1358710	0.817180	3.769750e-52	-8.615850e-47
Msa0264640	Msa1358710	0.820017	8.563596e-53	-8.615850e-47
Msa0277600	Msa1358710	0.807667	4.519244e-50	-8.615850e-47
Msa0301220	Msa1358710	0.817016	4.103082e-52	-8.615850e-47
Msa0301250	Msa1358710	0.819056	1.418731e-52	-8.615850e-47
Msa0313060	Msa1358710	0.830180	3.395728e-55	-8.615850e-47
Msa0340560	Msa1358710	0.808390	3.170568e-50	-8.615850e-47
Msa1166570	Msa1358710	0.817107	3.915025e-52	-8.615850e-47
Msa0511760	Msa1358710	0.814230	1.712541e-51	-8.615850e-47
Msa0517480	Msa1358710	0.836671	8.162048e-57	-8.615850e-47
Msa0540480	Msa1358710	0.801746	7.783969e-49	-8.615850e-47
Msa0547920	Msa1358710	0.821406	4.105746e-53	-8.615850e-47
Msa0573550	Msa1358710	0.833554	4.989298e-56	-8.615850e-47
Msa0597240	Msa1358710	0.822652	2.112316e-53	-8.615850e-47
Msa0929680	Msa1358710	0.847401	1.183554e-59	-8.615850e-47
Msa0929700	Msa1358710	0.835912	1.273134e-56	-8.615850e-47
Msa0930360	Msa1358710	0.809351	1.975306e-50	-8.615850e-47
Msa0930790	Msa1358710	0.824386	8.300482e-54	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1358710	MtrunA17_Chr5g0401191	100.000	146	0	0	1	146	1	146	2.53e-103	291
Msa1358710	MtrunA17_Chr2g0281331	95.205	146	7	0	1	146	1	146	2.14e-90	259
Msa1358710	MtrunA17_Chr5g0447011	91.781	146	12	0	1	146	1	146	6.44e-88	253
Msa1358710	MtrunA17_Chr8g0370201	60.465	86	7	1	30	115	1	59	7.01e-25	90.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1358710	AT5G67510.1	86.301	146	20	0	1	146	1	146	2.54e-91	261
Msa1358710	AT3G49910.1	85.616	146	21	0	1	146	1	146	2.54e-91	261

Find 47 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCCCTTCACGTCCCTTGA+AGG	0.334674	tig0016374:-31137	None:intergenic
TGCAAGTTTATCGCAGGAAA+TGG	0.344368	tig0016374:+31164	Msa1358710:CDS
TACCAAGATTCGTTTGGATA+AGG	0.385625	tig0016374:+31270	Msa1358710:CDS
ATCCTTATCCAAACGAATCT+TGG	0.396970	tig0016374:-31272	None:intergenic
AAAACACGACGGACGCTTGA+TGG	0.398070	tig0016374:-31019	None:intergenic
ATTACACGTGAGAAGGTTAA+TGG	0.405029	tig0016374:+31205	Msa1358710:CDS
TGTGATTACCAAGATTCGTT+TGG	0.408401	tig0016374:+31264	Msa1358710:CDS
TTGGTAATCACAACCTTTGA+AGG	0.416639	tig0016374:-31253	None:intergenic
GGGTCGTGCCGCTGCTGATA+AGG	0.421050	tig0016374:+31324	Msa1358710:CDS
TCGTAAGGATGATGAAGTTC+AGG	0.424559	tig0016374:+31102	Msa1358710:CDS
CGACGGCTGCTTGAAACCCT+AGG	0.429571	tig0016374:-30968	None:intergenic
TGCCGCTGCTGATAAGGAAA+AGG	0.460452	tig0016374:+31330	Msa1358710:CDS
AAACACGACGGACGCTTGAT+GGG	0.465655	tig0016374:-31018	None:intergenic
GCGTTCTCTTCCCGTTCGTA+AGG	0.475960	tig0016374:+31087	Msa1358710:CDS
AATGTCTTCAGGAGCAAACT+TGG	0.478032	tig0016374:-31356	None:intergenic
GCAAGTTTATCGCAGGAAAT+GGG	0.492572	tig0016374:+31165	Msa1358710:CDS
GAAAAGCTTGCTTGATCGTA+AGG	0.501897	tig0016374:+31297	Msa1358710:CDS
ACTTCATCATCCTTACGAAC+GGG	0.506464	tig0016374:-31097	None:intergenic
TTGATGAGCGCACCTCTCTC+CGG	0.508962	tig0016374:+31040	Msa1358710:CDS
ACACAATGAAGTTCAACCCT+AGG	0.518183	tig0016374:+30951	None:intergenic
GGTAGTGAGAGGAACCTTCA+AGG	0.522192	tig0016374:+31123	Msa1358710:CDS
GTACTTTGAACGGAGGTCAC+CGG	0.530458	tig0016374:-31059	None:intergenic
CAGCCGTCGCAAGAGCCGCA+AGG	0.548428	tig0016374:+30982	Msa1358710:CDS
CACAATGAAGTTCAACCCTA+GGG	0.551275	tig0016374:+30952	None:intergenic
GCCGCTGCTGATAAGGAAAA+GGG	0.554219	tig0016374:+31331	Msa1358710:CDS
AATGGGTGATTCATATTGAG+AGG	0.559538	tig0016374:+31182	Msa1358710:CDS
AACTTCATCATCCTTACGAA+CGG	0.563073	tig0016374:-31098	None:intergenic
GGAACCTTCAAGGGACGTGA+AGG	0.563323	tig0016374:+31133	Msa1358710:CDS
GACGGCTGCTTGAAACCCTA+GGG	0.565492	tig0016374:-30967	None:intergenic
AGGTTGTGCAAGTTTATCGC+AGG	0.567275	tig0016374:+31158	Msa1358710:CDS
TGTTGGTGTTGATCCTTCAA+AGG	0.568879	tig0016374:+31240	Msa1358710:CDS
GAGCCTTGCGGCTCTTGCGA+CGG	0.572981	tig0016374:-30985	None:intergenic
CTTGCTTGATCGTAAGGCGA+AGG	0.575754	tig0016374:+31303	Msa1358710:CDS
AACGCACGTTGTACTTTGAA+CGG	0.578356	tig0016374:-31069	None:intergenic
AGATTCGTTTGGATAAGGAT+AGG	0.578817	tig0016374:+31275	Msa1358710:CDS
AACACGACGGACGCTTGATG+GGG	0.578903	tig0016374:-31017	None:intergenic
TGAGAGGATTACACGTGAGA+AGG	0.580146	tig0016374:+31198	Msa1358710:CDS
GATGAAGTTCAGGTAGTGAG+AGG	0.591688	tig0016374:+31112	Msa1358710:CDS
CTTCAAGGGACGTGAAGGGA+AGG	0.593141	tig0016374:+31138	Msa1358710:CDS
AATGGTCAAACTGTGAATGT+TGG	0.612366	tig0016374:+31223	Msa1358710:CDS
TTGCTTGATCGTAAGGCGAA+GGG	0.621055	tig0016374:+31304	Msa1358710:CDS
GAACGGAGGTCACCGGAGAG+AGG	0.625041	tig0016374:-31052	None:intergenic
GTAGTGAGAGGAACCTTCAA+GGG	0.634832	tig0016374:+31124	Msa1358710:CDS
GAACCTTCAAGGGACGTGAA+GGG	0.643186	tig0016374:+31134	Msa1358710:CDS
GCACGTTGTACTTTGAACGG+AGG	0.673729	tig0016374:-31066	None:intergenic
GGGCGGTGAAATGAGCCTTG+CGG	0.681827	tig0016374:-30997	None:intergenic
GTGCGCTCATCAAAACACGA+CGG	0.772319	tig0016374:-31030	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACTTCATCATCCTTACGAA+CGG	-	tig0016374:31101-31120	None:intergenic	35.0%
	AATGGGTGATTCATATTGAG+AGG	+	tig0016374:31182-31201	Msa1358710:CDS	35.0%
	ATTACACGTGAGAAGGTTAA+TGG	+	tig0016374:31205-31224	Msa1358710:CDS	35.0%
	AATGGTCAAACTGTGAATGT+TGG	+	tig0016374:31223-31242	Msa1358710:CDS	35.0%
!	TTGGTAATCACAACCTTTGA+AGG	-	tig0016374:31256-31275	None:intergenic	35.0%
	TGTGATTACCAAGATTCGTT+TGG	+	tig0016374:31264-31283	Msa1358710:CDS	35.0%
	TACCAAGATTCGTTTGGATA+AGG	+	tig0016374:31270-31289	Msa1358710:CDS	35.0%
	ATCCTTATCCAAACGAATCT+TGG	-	tig0016374:31275-31294	None:intergenic	35.0%
	AGATTCGTTTGGATAAGGAT+AGG	+	tig0016374:31275-31294	Msa1358710:CDS	35.0%
!	ACGTTTTGCATAATGTCTTC+AGG	-	tig0016374:31370-31389	None:intergenic	35.0%
	AACGCACGTTGTACTTTGAA+CGG	-	tig0016374:31072-31091	None:intergenic	40.0%
	ACTTCATCATCCTTACGAAC+GGG	-	tig0016374:31100-31119	None:intergenic	40.0%
	TCGTAAGGATGATGAAGTTC+AGG	+	tig0016374:31102-31121	Msa1358710:CDS	40.0%
	TGCAAGTTTATCGCAGGAAA+TGG	+	tig0016374:31164-31183	Msa1358710:CDS	40.0%
	GCAAGTTTATCGCAGGAAAT+GGG	+	tig0016374:31165-31184	Msa1358710:CDS	40.0%
!!	TGTTGGTGTTGATCCTTCAA+AGG	+	tig0016374:31240-31259	Msa1358710:CDS	40.0%
!	GAAAAGCTTGCTTGATCGTA+AGG	+	tig0016374:31297-31316	Msa1358710:CDS	40.0%
	AATGTCTTCAGGAGCAAACT+TGG	-	tig0016374:31359-31378	None:intergenic	40.0%
	GATGAAGTTCAGGTAGTGAG+AGG	+	tig0016374:31112-31131	Msa1358710:CDS	45.0%
	GTAGTGAGAGGAACCTTCAA+GGG	+	tig0016374:31124-31143	Msa1358710:CDS	45.0%
	AGGTTGTGCAAGTTTATCGC+AGG	+	tig0016374:31158-31177	Msa1358710:CDS	45.0%
	TGAGAGGATTACACGTGAGA+AGG	+	tig0016374:31198-31217	Msa1358710:CDS	45.0%
	TTGCTTGATCGTAAGGCGAA+GGG	+	tig0016374:31304-31323	Msa1358710:CDS	45.0%
!	ACCCTTTTCCTTATCAGCAG+CGG	-	tig0016374:31335-31354	None:intergenic	45.0%
	AAACACGACGGACGCTTGAT+GGG	-	tig0016374:31021-31040	None:intergenic	50.0%
	AAAACACGACGGACGCTTGA+TGG	-	tig0016374:31022-31041	None:intergenic	50.0%
	GTGCGCTCATCAAAACACGA+CGG	-	tig0016374:31033-31052	None:intergenic	50.0%
	GTACTTTGAACGGAGGTCAC+CGG	-	tig0016374:31062-31081	None:intergenic	50.0%
	GCACGTTGTACTTTGAACGG+AGG	-	tig0016374:31069-31088	None:intergenic	50.0%
	GGTAGTGAGAGGAACCTTCA+AGG	+	tig0016374:31123-31142	Msa1358710:CDS	50.0%
	GAACCTTCAAGGGACGTGAA+GGG	+	tig0016374:31134-31153	Msa1358710:CDS	50.0%
	CTTGCTTGATCGTAAGGCGA+AGG	+	tig0016374:31303-31322	Msa1358710:CDS	50.0%
	TGCCGCTGCTGATAAGGAAA+AGG	+	tig0016374:31330-31349	Msa1358710:CDS	50.0%
	GCCGCTGCTGATAAGGAAAA+GGG	+	tig0016374:31331-31350	Msa1358710:CDS	50.0%
	GACGGCTGCTTGAAACCCTA+GGG	-	tig0016374:30970-30989	None:intergenic	55.0%
	AACACGACGGACGCTTGATG+GGG	-	tig0016374:31020-31039	None:intergenic	55.0%
	TTGATGAGCGCACCTCTCTC+CGG	+	tig0016374:31040-31059	Msa1358710:CDS	55.0%
	GCGTTCTCTTCCCGTTCGTA+AGG	+	tig0016374:31087-31106	Msa1358710:CDS	55.0%
	GGAACCTTCAAGGGACGTGA+AGG	+	tig0016374:31133-31152	Msa1358710:CDS	55.0%
	CTTCCCTTCACGTCCCTTGA+AGG	-	tig0016374:31140-31159	None:intergenic	55.0%
	CTTCAAGGGACGTGAAGGGA+AGG	+	tig0016374:31138-31157	Msa1358710:CDS	55.0%
	CGACGGCTGCTTGAAACCCT+AGG	-	tig0016374:30971-30990	None:intergenic	60.0%
	GGGCGGTGAAATGAGCCTTG+CGG	-	tig0016374:31000-31019	None:intergenic	60.0%
	GAGCCTTGCGGCTCTTGCGA+CGG	-	tig0016374:30988-31007	None:intergenic	65.0%
	ACGACGGACGCTTGATGGGG+CGG	-	tig0016374:31017-31036	None:intergenic	65.0%
	GAACGGAGGTCACCGGAGAG+AGG	-	tig0016374:31055-31074	None:intergenic	65.0%
!	GGGTCGTGCCGCTGCTGATA+AGG	+	tig0016374:31324-31343	Msa1358710:CDS	65.0%
	CAGCCGTCGCAAGAGCCGCA+AGG	+	tig0016374:30982-31001	Msa1358710:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
tig0016374	gene	30956	31396	30956	ID=Msa1358710;Name=Msa1358710
tig0016374	mRNA	30956	31396	30956	ID=Msa1358710-mRNA-1;Parent=Msa1358710;Name=Msa1358710-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|146
tig0016374	exon	30956	31396	30956	ID=Msa1358710-mRNA-1:exon:10982;Parent=Msa1358710-mRNA-1
tig0016374	CDS	30956	31396	30956	ID=Msa1358710-mRNA-1:cds;Parent=Msa1358710-mRNA-1

Gene Sequence

>Msa1358710

ATGAAGTTCAACCCTAGGGTTTCAAGCAGCCGTCGCAAGAGCCGCAAGGCTCATTTCACCGCCCCATCAAGCGTCCGTCGTGTTTTGATGAGCGCACCTCTCTCCGGTGACCTCCGTTCAAAGTACAACGTGCGTTCTCTTCCCGTTCGTAAGGATGATGAAGTTCAGGTAGTGAGAGGAACCTTCAAGGGACGTGAAGGGAAGGTTGTGCAAGTTTATCGCAGGAAATGGGTGATTCATATTGAGAGGATTACACGTGAGAAGGTTAATGGTCAAACTGTGAATGTTGGTGTTGATCCTTCAAAGGTTGTGATTACCAAGATTCGTTTGGATAAGGATAGGAAAAGCTTGCTTGATCGTAAGGCGAAGGGTCGTGCCGCTGCTGATAAGGAAAAGGGTACCAAGTTTGCTCCTGAAGACATTATGCAAAACGTTGATTAG

Protein sequence

>Msa1358710

MKFNPRVSSSRRKSRKAHFTAPSSVRRVLMSAPLSGDLRSKYNVRSLPVRKDDEVQVVRGTFKGREGKVVQVYRRKWVIHIERITREKVNGQTVNVGVDPSKVVITKIRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQNVD*