Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
Msa1457850 | GAU50027.1 | 83.051 | 59 | 10 | 0 | 1 | 59 | 1 | 59 | 9.91e-25 | 96.7 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
Msa1457850 | A0A2Z6PTB5 | 83.051 | 59 | 10 | 0 | 1 | 59 | 1 | 59 | 4.73e-25 | 96.7 |
Gene ID | Type | Classification |
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Gene ID | Type | Classification |
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Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
Msa0004590 | Msa1457850 | 0.808779 | 2.618504e-50 | -8.615850e-47 |
Msa0009530 | Msa1457850 | -0.810162 | 1.321925e-50 | -8.615850e-47 |
Msa0014590 | Msa1457850 | 0.802222 | 6.213945e-49 | -8.615850e-47 |
Msa0032390 | Msa1457850 | 0.806340 | 8.627244e-50 | -8.615850e-47 |
Msa0057860 | Msa1457850 | 0.815874 | 7.391908e-52 | -8.615850e-47 |
Msa0092220 | Msa1457850 | 0.802313 | 5.953354e-49 | -8.615850e-47 |
Msa0102920 | Msa1457850 | 0.806059 | 9.885232e-50 | -8.615850e-47 |
Msa1370970 | Msa1457850 | 0.800732 | 1.255271e-48 | -8.615850e-47 |
Msa1380740 | Msa1457850 | 0.806536 | 7.845242e-50 | -8.615850e-47 |
Msa1395050 | Msa1457850 | 0.818905 | 1.535625e-52 | -8.615850e-47 |
Msa1413020 | Msa1457850 | 0.801858 | 7.382525e-49 | -8.615850e-47 |
Msa1447900 | Msa1457850 | 0.805272 | 1.445918e-49 | -8.615850e-47 |
Msa0609460 | Msa1457850 | 0.801468 | 8.876104e-49 | -8.615850e-47 |
Msa0623310 | Msa1457850 | 0.802985 | 4.326427e-49 | -8.615850e-47 |
Msa0624930 | Msa1457850 | 0.815990 | 6.963854e-52 | -8.615850e-47 |
Msa0631700 | Msa1457850 | 0.806179 | 9.328667e-50 | -8.615850e-47 |
Msa0643190 | Msa1457850 | 0.804979 | 1.665642e-49 | -8.615850e-47 |
Msa0650100 | Msa1457850 | 0.803390 | 3.566892e-49 | -8.615850e-47 |
Msa0658120 | Msa1457850 | 0.818978 | 1.478359e-52 | -8.615850e-47 |
Msa0670360 | Msa1457850 | 0.801587 | 8.392185e-49 | -8.615850e-47 |
Msa0671400 | Msa1457850 | 0.824302 | 8.689609e-54 | -8.615850e-47 |
Msa0702970 | Msa1457850 | 0.807133 | 5.866269e-50 | -8.615850e-47 |
Msa0716700 | Msa1457850 | 0.808420 | 3.124704e-50 | -8.615850e-47 |
Msa0742200 | Msa1457850 | 0.826948 | 2.050121e-54 | -8.615850e-47 |
Msa0804170 | Msa1457850 | 0.802638 | 5.101613e-49 | -8.615850e-47 |
Msa0806930 | Msa1457850 | 0.801421 | 9.077641e-49 | -8.615850e-47 |
Msa0814160 | Msa1457850 | 0.800703 | 1.272596e-48 | -8.615850e-47 |
Msa0823380 | Msa1457850 | 0.823117 | 1.646001e-53 | -8.615850e-47 |
Msa0147680 | Msa1457850 | 0.834699 | 2.577604e-56 | -8.615850e-47 |
Msa0190890 | Msa1457850 | 0.833435 | 5.342351e-56 | -8.615850e-47 |
Msa0191100 | Msa1457850 | 0.800210 | 1.604083e-48 | -8.615850e-47 |
Msa0195580 | Msa1457850 | 0.802639 | 5.100512e-49 | -8.615850e-47 |
Msa0220090 | Msa1457850 | 0.814693 | 1.352726e-51 | -8.615850e-47 |
Msa0225070 | Msa1457850 | -0.800799 | 1.216539e-48 | -8.615850e-47 |
Msa0226410 | Msa1457850 | 0.804486 | 2.111378e-49 | -8.615850e-47 |
Msa0233210 | Msa1457850 | 0.817509 | 3.177990e-52 | -8.615850e-47 |
Msa0238110 | Msa1457850 | 0.815496 | 8.972850e-52 | -8.615850e-47 |
Msa1225890 | Msa1457850 | 0.846318 | 2.342012e-59 | -8.615850e-47 |
Msa1281780 | Msa1457850 | 0.811041 | 8.538508e-51 | -8.615850e-47 |
Msa1323260 | Msa1457850 | -0.810095 | 1.366776e-50 | -8.615850e-47 |
Msa1343590 | Msa1457850 | 0.802868 | 4.573435e-49 | -8.615850e-47 |
Msa0379480 | Msa1457850 | 0.803799 | 2.933690e-49 | -8.615850e-47 |
Msa0452770 | Msa1457850 | 0.815725 | 7.978227e-52 | -8.615850e-47 |
Msa0472390 | Msa1457850 | 0.806070 | 9.833947e-50 | -8.615850e-47 |
Msa1015560 | Msa1457850 | 0.803239 | 3.832309e-49 | -8.615850e-47 |
Msa1061760 | Msa1457850 | 0.807585 | 4.703545e-50 | -8.615850e-47 |
Msa1066150 | Msa1457850 | 0.821883 | 3.186409e-53 | -8.615850e-47 |
Msa1078590 | Msa1457850 | -0.800008 | 1.762500e-48 | -8.615850e-47 |
Msa1098100 | Msa1457850 | 0.805068 | 1.595970e-49 | -8.615850e-47 |
Msa0248320 | Msa1457850 | 0.800085 | 1.700373e-48 | -8.615850e-47 |
Msa0261400 | Msa1457850 | 0.802997 | 4.300829e-49 | -8.615850e-47 |
Msa0267710 | Msa1457850 | 0.814506 | 1.488180e-51 | -8.615850e-47 |
Msa0311540 | Msa1457850 | 0.811011 | 8.663841e-51 | -8.615850e-47 |
Msa0349930 | Msa1457850 | 0.808509 | 2.990166e-50 | -8.615850e-47 |
Msa0358470 | Msa1457850 | 0.810835 | 9.461129e-51 | -8.615850e-47 |
Msa1107330 | Msa1457850 | 0.832204 | 1.080269e-55 | -8.615850e-47 |
Msa1130770 | Msa1457850 | 0.803081 | 4.131720e-49 | -8.615850e-47 |
Msa1138110 | Msa1457850 | 0.813834 | 2.094646e-51 | -8.615850e-47 |
Msa1150860 | Msa1457850 | 0.809551 | 1.789594e-50 | -8.615850e-47 |
Msa1186260 | Msa1457850 | 0.812747 | 3.629452e-51 | -8.615850e-47 |
Msa0515150 | Msa1457850 | 0.800958 | 1.128811e-48 | -8.615850e-47 |
Msa0529360 | Msa1457850 | 0.813264 | 2.795258e-51 | -8.615850e-47 |
Msa0535570 | Msa1457850 | 0.802133 | 6.482006e-49 | -8.615850e-47 |
Msa0565700 | Msa1457850 | 0.800488 | 1.407907e-48 | -8.615850e-47 |
Msa0566460 | Msa1457850 | 0.826802 | 2.221723e-54 | -8.615850e-47 |
Msa0566930 | Msa1457850 | 0.817432 | 3.308454e-52 | -8.615850e-47 |
Msa0583390 | Msa1457850 | 0.846820 | 1.707859e-59 | -8.615850e-47 |
Msa0591490 | Msa1457850 | 0.807574 | 4.729738e-50 | -8.615850e-47 |
Msa0602050 | Msa1457850 | 0.817987 | 2.479550e-52 | -8.615850e-47 |
Msa0880910 | Msa1457850 | 0.805848 | 1.095018e-49 | -8.615850e-47 |
Msa0907840 | Msa1457850 | 0.990411 | 1.657794e-182 | -8.615850e-47 |
Msa0932140 | Msa1457850 | 0.820287 | 7.428497e-53 | -8.615850e-47 |
Msa0932150 | Msa1457850 | 0.804892 | 1.736619e-49 | -8.615850e-47 |
Msa0940780 | Msa1457850 | 0.800552 | 1.365900e-48 | -8.615850e-47 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
Msa1457850 | MtrunA17_Chr6g0461381 | 93.220 | 59 | 4 | 0 | 1 | 59 | 1 | 59 | 1.13e-21 | 79.0 |
Msa1457850 | MtrunA17_Chr7g0253381 | 69.492 | 59 | 18 | 0 | 1 | 59 | 1 | 59 | 1.79e-21 | 78.2 |
Msa1457850 | MtrunA17_Chr6g0461421 | 94.915 | 59 | 3 | 0 | 1 | 59 | 1 | 59 | 3.77e-21 | 77.4 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Find 7 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
AGAGTAGTAGCATCTGATGT+AGG | 0.452027 | tig0039241:-19301 | Msa1457850:intergenic |
ACAAAGGAAGCAATTATTGT+TGG | 0.473703 | tig0039241:-19331 | Msa1457850:intergenic |
GTAGCATCTGATGTAGGGCT+TGG | 0.485712 | tig0039241:-19295 | Msa1457850:intergenic |
GAGTAGTAGCATCTGATGTA+GGG | 0.524730 | tig0039241:-19300 | Msa1457850:intergenic |
GGTGGTGGTGGCCATGATGA+TGG | 0.538657 | tig0039241:+19237 | Msa1457850:CDS |
ACTTGATGCTGCCATCATCA+TGG | 0.566779 | tig0039241:-19248 | Msa1457840:intergenic |
TCTGATGTAGGGCTTGGAGC+TGG | 0.570335 | tig0039241:-19289 | Msa1457840:intergenic |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
!! | GATGAATCAAATTTTTGCTA+TGG | + | tig0039241:19216-19235 | Msa1457850:CDS | 25.0% |
!! | GAATCAAATTTTTGCTATGG+TGG | + | tig0039241:19219-19238 | Msa1457850:CDS | 30.0% |
ACAAAGGAAGCAATTATTGT+TGG | - | tig0039241:19334-19353 | Msa1457850:intergenic | 30.0% | |
!! | TCAAATTTTTGCTATGGTGG+TGG | + | tig0039241:19222-19241 | Msa1457850:CDS | 35.0% |
!!! | TCCTTTGTTGCTCTTGTTTT+TGG | + | tig0039241:19346-19365 | Msa1457850:CDS | 35.0% |
TCCAAAAACAAGAGCAACAA+AGG | - | tig0039241:19350-19369 | Msa1457850:intergenic | 35.0% | |
!!! | AATTTTTGCTATGGTGGTGG+TGG | + | tig0039241:19225-19244 | Msa1457850:CDS | 40.0% |
GAGTAGTAGCATCTGATGTA+GGG | - | tig0039241:19303-19322 | Msa1457850:intergenic | 40.0% | |
AGAGTAGTAGCATCTGATGT+AGG | - | tig0039241:19304-19323 | Msa1457850:intergenic | 40.0% | |
ACTTGATGCTGCCATCATCA+TGG | - | tig0039241:19251-19270 | Msa1457850:intergenic | 45.0% | |
GTAGCATCTGATGTAGGGCT+TGG | - | tig0039241:19298-19317 | Msa1457850:intergenic | 50.0% | |
TCTGATGTAGGGCTTGGAGC+TGG | - | tig0039241:19292-19311 | Msa1457850:intergenic | 55.0% | |
!! | GGTGGTGGTGGCCATGATGA+TGG | + | tig0039241:19237-19256 | Msa1457850:CDS | 60.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
tig0039241 | gene | 19202 | 19381 | 19202 | ID=Msa1457850;Name=Msa1457850 |
tig0039241 | mRNA | 19202 | 19381 | 19202 | ID=Msa1457850-mRNA-1;Parent=Msa1457850;Name=Msa1457850-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|59 |
tig0039241 | exon | 19202 | 19381 | 19202 | ID=Msa1457850-mRNA-1:exon:21434;Parent=Msa1457850-mRNA-1 |
tig0039241 | CDS | 19202 | 19381 | 19202 | ID=Msa1457850-mRNA-1:cds;Parent=Msa1457850-mRNA-1 |
Gene Sequence |
Protein sequence |