AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1457850


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1457850 MtrunA17_Chr6g0461381 93.220 59 4 0 1 59 1 59 1.13e-21 79.0
Msa1457850 MtrunA17_Chr7g0253381 69.492 59 18 0 1 59 1 59 1.79e-21 78.2
Msa1457850 MtrunA17_Chr6g0461421 94.915 59 3 0 1 59 1 59 3.77e-21 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 7 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGTAGTAGCATCTGATGT+AGG 0.452027 tig0039241:-19301 Msa1457850:intergenic
ACAAAGGAAGCAATTATTGT+TGG 0.473703 tig0039241:-19331 Msa1457850:intergenic
GTAGCATCTGATGTAGGGCT+TGG 0.485712 tig0039241:-19295 Msa1457850:intergenic
GAGTAGTAGCATCTGATGTA+GGG 0.524730 tig0039241:-19300 Msa1457850:intergenic
GGTGGTGGTGGCCATGATGA+TGG 0.538657 tig0039241:+19237 Msa1457850:CDS
ACTTGATGCTGCCATCATCA+TGG 0.566779 tig0039241:-19248 Msa1457840:intergenic
TCTGATGTAGGGCTTGGAGC+TGG 0.570335 tig0039241:-19289 Msa1457840:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATGAATCAAATTTTTGCTA+TGG + tig0039241:19216-19235 Msa1457850:CDS 25.0%
!! GAATCAAATTTTTGCTATGG+TGG + tig0039241:19219-19238 Msa1457850:CDS 30.0%
ACAAAGGAAGCAATTATTGT+TGG - tig0039241:19334-19353 Msa1457850:intergenic 30.0%
!! TCAAATTTTTGCTATGGTGG+TGG + tig0039241:19222-19241 Msa1457850:CDS 35.0%
!!! TCCTTTGTTGCTCTTGTTTT+TGG + tig0039241:19346-19365 Msa1457850:CDS 35.0%
TCCAAAAACAAGAGCAACAA+AGG - tig0039241:19350-19369 Msa1457850:intergenic 35.0%
!!! AATTTTTGCTATGGTGGTGG+TGG + tig0039241:19225-19244 Msa1457850:CDS 40.0%
GAGTAGTAGCATCTGATGTA+GGG - tig0039241:19303-19322 Msa1457850:intergenic 40.0%
AGAGTAGTAGCATCTGATGT+AGG - tig0039241:19304-19323 Msa1457850:intergenic 40.0%
ACTTGATGCTGCCATCATCA+TGG - tig0039241:19251-19270 Msa1457850:intergenic 45.0%
GTAGCATCTGATGTAGGGCT+TGG - tig0039241:19298-19317 Msa1457850:intergenic 50.0%
TCTGATGTAGGGCTTGGAGC+TGG - tig0039241:19292-19311 Msa1457850:intergenic 55.0%
!! GGTGGTGGTGGCCATGATGA+TGG + tig0039241:19237-19256 Msa1457850:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0039241 gene 19202 19381 19202 ID=Msa1457850;Name=Msa1457850
tig0039241 mRNA 19202 19381 19202 ID=Msa1457850-mRNA-1;Parent=Msa1457850;Name=Msa1457850-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|59
tig0039241 exon 19202 19381 19202 ID=Msa1457850-mRNA-1:exon:21434;Parent=Msa1457850-mRNA-1
tig0039241 CDS 19202 19381 19202 ID=Msa1457850-mRNA-1:cds;Parent=Msa1457850-mRNA-1
Gene Sequence

>Msa1457850

ATGGAGGCATTGAAGATGAATCAAATTTTTGCTATGGTGGTGGTGGCCATGATGATGGCAGCATCAAGTGTTTCAGCTGCAGATGCACCAGCTCCAAGCCCTACATCAGATGCTACTACTCTCTTTGTGCCAACAATAATTGCTTCCTTTGTTGCTCTTGTTTTTGGATATCTTTTCTAA

Protein sequence

>Msa1457850

MEALKMNQIFAMVVVAMMMAASSVSAADAPAPSPTSDATTLFVPTIIASFVALVFGYLF*