AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1463820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1463820 MtrunA17_Chr5g0394991 87.736 212 6 1 1 192 1 212 1.70e-125 352
Msa1463820 MtrunA17_Chr1g0205171 49.541 218 83 4 1 192 1 217 2.02e-58 182
Msa1463820 MtrunA17_Chr1g0185311 46.809 141 68 7 1 137 1 138 2.58e-30 109
Msa1463820 MtrunA17_Chr7g0260071 40.876 137 77 3 8 142 7 141 1.21e-16 74.7
Msa1463820 MtrunA17_Chr5g0395001 34.959 123 69 6 1 121 1 114 5.39e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1463820 AT3G22620.1 42.574 202 94 6 10 192 5 203 3.24e-35 123
Msa1463820 AT1G05450.2 44.751 181 74 5 26 188 27 199 1.37e-34 121
Msa1463820 AT2G48140.2 54.310 116 47 3 12 124 7 119 2.63e-34 118
Msa1463820 AT2G48140.1 41.294 201 88 7 12 190 7 199 6.11e-34 119
Msa1463820 AT3G22600.1 34.815 135 69 6 1 125 1 126 2.60e-12 62.8
Msa1463820 AT2G48130.2 32.124 193 101 10 4 184 3 177 3.87e-12 62.4
Msa1463820 AT2G48130.6 32.124 193 101 10 4 184 3 177 3.87e-12 62.4
Msa1463820 AT2G48130.4 32.124 193 101 10 4 184 3 177 3.87e-12 62.4
Msa1463820 AT2G48130.5 32.124 193 101 10 4 184 3 177 3.87e-12 62.4
Msa1463820 AT2G48130.1 32.124 193 101 10 4 184 3 177 3.87e-12 62.4
Msa1463820 AT2G48130.3 32.124 193 101 10 4 184 3 177 3.87e-12 62.4

Find 74 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATTGTTTGGACCCATAT+TGG 0.216171 tig0043655:+2065 Msa1463820:three_prime_UTR
AAACCAATACCACCCCAATA+TGG 0.274848 tig0043655:-2079 Msa1463820:intergenic
GACCCATATTGGGGTGGTAT+TGG 0.297176 tig0043655:+2076 Msa1463820:three_prime_UTR
AAATTGTTTGGACCCATATT+GGG 0.299454 tig0043655:+2066 Msa1463820:three_prime_UTR
CACAACAATACAAATGTCTT+TGG 0.304309 tig0043655:-2105 Msa1463820:intergenic
CAGATCAGAGCGGCCACTTC+CGG 0.318856 tig0043655:-1866 Msa1463820:intergenic
TAGTCTCCTACTATCTGTTT+TGG 0.329233 tig0043655:+1918 Msa1463820:CDS
TTAAAACACTACTAGGTTAC+TGG 0.335706 tig0043655:+1958 Msa1463820:exon
CTCTATAACGTAAACATAGA+TGG 0.351516 tig0043655:+840 Msa1463820:exon
AATATGTTTGGCTATATTGC+AGG 0.361101 tig0043655:+2030 Msa1463820:three_prime_UTR
GGCGTTGCAGATGGAATATC+AGG 0.363139 tig0043655:-1844 Msa1463820:intergenic
ATGTTTACGTTATAGAGTTT+TGG 0.368636 tig0043655:-834 Msa1463820:intergenic
ATAGGTAGTTGCAATATGTT+TGG 0.375312 tig0043655:+2018 Msa1463820:three_prime_UTR
GGGTTCGCTGATGGAGATAA+TGG 0.387464 tig0043655:-1823 Msa1463820:intergenic
ATAGCTAAGGTTCTATTGAT+TGG 0.389392 tig0043655:-1126 Msa1463820:intergenic
TTCTATATTAAAACACTACT+AGG 0.400865 tig0043655:+1951 Msa1463820:CDS
GGCTCACCGCTTCCTGCTCC+AGG 0.422121 tig0043655:+1324 Msa1463820:CDS
GATCTGACTCCATCATCTGC+TGG 0.433930 tig0043655:+1883 Msa1463820:CDS
GAAACTCAAGCAAGGGCTAA+TGG 0.441100 tig0043655:-977 Msa1463820:intergenic
CCTCGTGCTTGTAACTTGCC+TGG 0.444455 tig0043655:+1156 Msa1463820:CDS
AACCAATACCACCCCAATAT+GGG 0.447031 tig0043655:-2078 Msa1463820:intergenic
CCACTTCCGGGCGTTGCAGA+TGG 0.451493 tig0043655:-1853 Msa1463820:intergenic
CTATTGATTGGTAATGCAAA+AGG 0.452479 tig0043655:-1114 Msa1463820:intergenic
GTTAGCCGTGGCTTCGATCA+TGG 0.462490 tig0043655:+897 Msa1463820:CDS
TGATACATTAAAATCAATAG+AGG 0.466281 tig0043655:-1990 Msa1463820:intergenic
CTTACCATTGATATGTTACA+TGG 0.470781 tig0043655:-949 Msa1463820:intergenic
CAATGACCAAAACAGATAGT+AGG 0.505531 tig0043655:-1924 Msa1463820:intergenic
GATAATGGATTGGTTGTCTC+AGG 0.511804 tig0043655:-1808 Msa1463820:intergenic
TTTGTTGTGTTTAAGAGTGA+AGG 0.513809 tig0043655:-788 Msa1463820:intergenic
ATCCTTGCTTCCACTTGTGA+GGG 0.516520 tig0043655:-1061 Msa1463820:intergenic
CCATCTGCAACGCCCGGAAG+TGG 0.516819 tig0043655:+1853 Msa1463820:CDS
GATGATGATCCAGCAGATGA+TGG 0.519019 tig0043655:-1892 Msa1463820:intergenic
AATCCTTGCTTCCACTTGTG+AGG 0.527769 tig0043655:-1062 Msa1463820:intergenic
GGAATATCAGGGTTCGCTGA+TGG 0.537454 tig0043655:-1832 Msa1463820:intergenic
AGAGAAGGTGTCACTGGTGA+GGG 0.537488 tig0043655:-1775 Msa1463820:intergenic
CCACGGCTAACACCACAGTT+AGG 0.542248 tig0043655:-885 Msa1463820:intergenic
CGCTGATGGAGATAATGGAT+TGG 0.542515 tig0043655:-1818 Msa1463820:intergenic
GATATTCCATCTGCAACGCC+CGG 0.544541 tig0043655:+1847 Msa1463820:CDS
CTCACAAATAGTAGTGGCAA+TGG 0.545441 tig0043655:+1000 Msa1463820:CDS
CGTCGCGGTTTACCTGGAGC+AGG 0.545706 tig0043655:-1336 Msa1463820:intergenic
GACCCTCACAAGTGGAAGCA+AGG 0.549047 tig0043655:+1059 Msa1463820:CDS
CATTGCCACTACTATTTGTG+AGG 0.549899 tig0043655:-999 Msa1463820:intergenic
GTCGTGATTTGAGCGTAGGC+TGG 0.551456 tig0043655:-925 Msa1463820:intergenic
GTTTGGCTATATTGCAGGGA+GGG 0.557553 tig0043655:+2035 Msa1463820:three_prime_UTR
TGTATGTGTCTTATTGCCAC+TGG 0.565408 tig0043655:+1084 Msa1463820:CDS
GGATTGGTTGTCTCAGGCTG+TGG 0.565431 tig0043655:-1802 Msa1463820:intergenic
GGTTTACCTGGAGCAGGAAG+CGG 0.566105 tig0043655:-1330 Msa1463820:intergenic
GACGCCATGTAACATATCAA+TGG 0.568392 tig0043655:+945 Msa1463820:CDS
AATTGTTTGGACCCATATTG+GGG 0.569765 tig0043655:+2067 Msa1463820:three_prime_UTR
TTGCATTGAAGTGGAACACC+AGG 0.570114 tig0043655:-1174 Msa1463820:intergenic
GCAGCAATCAGCAGTGGGTG+AGG 0.573593 tig0043655:-1025 Msa1463820:intergenic
TGGCGTCGTGATTTGAGCGT+AGG 0.576088 tig0043655:-929 Msa1463820:intergenic
GCAATCAAGACCCTCACAAG+TGG 0.578959 tig0043655:+1051 Msa1463820:CDS
CAGAGAAGGTGTCACTGGTG+AGG 0.580166 tig0043655:-1776 Msa1463820:intergenic
GTATTGTTGTGTATTATGTG+TGG 0.581727 tig0043655:+2117 Msa1463820:three_prime_UTR
ATATGTTTGGCTATATTGCA+GGG 0.593280 tig0043655:+2031 Msa1463820:three_prime_UTR
AGTTTCCTCACAAATAGTAG+TGG 0.606601 tig0043655:+994 Msa1463820:CDS
TGCAGCCATGATCGAAGCCA+CGG 0.611380 tig0043655:-902 Msa1463820:intergenic
CCTAACTGTGGTGTTAGCCG+TGG 0.613375 tig0043655:+885 Msa1463820:CDS
GGTGACAGAGAAGGTGTCAC+TGG 0.619445 tig0043655:-1781 Msa1463820:intergenic
TGTTTGGACCCATATTGGGG+TGG 0.623606 tig0043655:+2070 Msa1463820:three_prime_UTR
TTGTGAGGAAACTCAAGCAA+GGG 0.634401 tig0043655:-984 Msa1463820:intergenic
ACGAGGAAGAGAGATAGCTA+AGG 0.635982 tig0043655:-1139 Msa1463820:intergenic
GCATTGCAGCAATCAGCAGT+GGG 0.640694 tig0043655:-1030 Msa1463820:intergenic
TTTGTGAGGAAACTCAAGCA+AGG 0.643831 tig0043655:-985 Msa1463820:intergenic
GTCTCTCTACCGCCTAACTG+TGG 0.648382 tig0043655:+873 Msa1463820:CDS
TCAGGCTGTGGTGACAGAGA+AGG 0.649180 tig0043655:-1790 Msa1463820:intergenic
TGCATTGCAGCAATCAGCAG+TGG 0.654060 tig0043655:-1031 Msa1463820:intergenic
GCGTTGCAGATGGAATATCA+GGG 0.683696 tig0043655:-1843 Msa1463820:intergenic
CCAGGCAAGTTACAAGCACG+AGG 0.683819 tig0043655:-1156 Msa1463820:intergenic
TGCAAAAGGAACATTGCCAG+TGG 0.683851 tig0043655:-1100 Msa1463820:intergenic
CGGCTAACACCACAGTTAGG+CGG 0.689758 tig0043655:-882 Msa1463820:intergenic
TGTTTGGCTATATTGCAGGG+AGG 0.699618 tig0043655:+2034 Msa1463820:three_prime_UTR
ATGATGGAGTCAGATCAGAG+CGG 0.809779 tig0043655:-1876 Msa1463810:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAAAATAATAGTAATTTTT+AGG - tig0043655:1377-1396 Msa1463820:intergenic 10.0%
!!! TTTTAATGTATCAAATTAAT+AGG + tig0043655:2000-2019 Msa1463820:three_prime_UTR 10.0%
!!! GAAAAATAATAGTAATTTTT+AGG - tig0043655:1377-1396 Msa1463820:intergenic 10.0%
!!! TTTTAATGTATCAAATTAAT+AGG + tig0043655:2000-2019 Msa1463820:three_prime_UTR 10.0%
!! AAAATAAAGAATGAAGATTT+AGG - tig0043655:1211-1230 Msa1463820:intergenic 15.0%
!!! ATAAATATGGTTTTAAATTG+CGG - tig0043655:1675-1694 Msa1463820:intergenic 15.0%
!! AAAATAAAGAATGAAGATTT+AGG - tig0043655:1211-1230 Msa1463820:intergenic 15.0%
!!! ATAAATATGGTTTTAAATTG+CGG - tig0043655:1675-1694 Msa1463820:intergenic 15.0%
!!! TTATAGAGTTTTGGTTTTTT+GGG - tig0043655:828-847 None:intergenic 20.0%
!! TTAGTGATCAACTATAAATA+TGG - tig0043655:1688-1707 Msa1463820:intergenic 20.0%
!! TTCTATATTAAAACACTACT+AGG + tig0043655:1951-1970 Msa1463820:CDS 20.0%
!! TGATACATTAAAATCAATAG+AGG - tig0043655:1993-2012 Msa1463820:intergenic 20.0%
!!! TTATAGAGTTTTGGTTTTTT+GGG - tig0043655:828-847 Msa1463820:intergenic 20.0%
!! TTAGTGATCAACTATAAATA+TGG - tig0043655:1688-1707 Msa1463820:intergenic 20.0%
!! TTCTATATTAAAACACTACT+AGG + tig0043655:1951-1970 Msa1463820:CDS 20.0%
!! TGATACATTAAAATCAATAG+AGG - tig0043655:1993-2012 Msa1463820:intergenic 20.0%
!!! TATAGAGTTTTGGTTTTTTG+GGG - tig0043655:827-846 None:intergenic 25.0%
!!! GTTATAGAGTTTTGGTTTTT+TGG - tig0043655:829-848 None:intergenic 25.0%
!! ATGTTTACGTTATAGAGTTT+TGG - tig0043655:837-856 None:intergenic 25.0%
! AGAGTAATTGACAAATAGAA+TGG - tig0043655:1273-1292 Msa1463820:intergenic 25.0%
!!! TCATTTTGACTTATATTGAC+AGG + tig0043655:1417-1436 Msa1463820:intron 25.0%
!!! GTGAAAAATTGTTTTTGATG+CGG + tig0043655:1575-1594 Msa1463820:intron 25.0%
!!! TATAGAGTTTTGGTTTTTTG+GGG - tig0043655:827-846 Msa1463820:intergenic 25.0%
!!! GTTATAGAGTTTTGGTTTTT+TGG - tig0043655:829-848 Msa1463820:intergenic 25.0%
!! ATGTTTACGTTATAGAGTTT+TGG - tig0043655:837-856 Msa1463820:intergenic 25.0%
! AGAGTAATTGACAAATAGAA+TGG - tig0043655:1273-1292 Msa1463820:intergenic 25.0%
!!! TCATTTTGACTTATATTGAC+AGG + tig0043655:1417-1436 Msa1463820:intron 25.0%
!!! GTGAAAAATTGTTTTTGATG+CGG + tig0043655:1575-1594 Msa1463820:intron 25.0%
! TTTGTTGTGTTTAAGAGTGA+AGG - tig0043655:791-810 None:intergenic 30.0%
CTCTATAACGTAAACATAGA+TGG + tig0043655:840-859 Msa1463820:exon 30.0%
CTTACCATTGATATGTTACA+TGG - tig0043655:952-971 Msa1463820:intergenic 30.0%
!! CTATTGATTGGTAATGCAAA+AGG - tig0043655:1117-1136 Msa1463820:intergenic 30.0%
ATAGCTAAGGTTCTATTGAT+TGG - tig0043655:1129-1148 Msa1463820:intergenic 30.0%
CTCAAGGTACATATTACTTA+CGG + tig0043655:1493-1512 Msa1463820:intron 30.0%
!!! TCACCTTTTTTTCATTGAAC+AGG + tig0043655:1739-1758 Msa1463820:intron 30.0%
GAACCTGTTCAATGAAAAAA+AGG - tig0043655:1745-1764 Msa1463820:intergenic 30.0%
TTAAAACACTACTAGGTTAC+TGG + tig0043655:1958-1977 Msa1463820:exon 30.0%
ATAGGTAGTTGCAATATGTT+TGG + tig0043655:2018-2037 Msa1463820:three_prime_UTR 30.0%
AATATGTTTGGCTATATTGC+AGG + tig0043655:2030-2049 Msa1463820:three_prime_UTR 30.0%
ATATGTTTGGCTATATTGCA+GGG + tig0043655:2031-2050 Msa1463820:three_prime_UTR 30.0%
AAATTGTTTGGACCCATATT+GGG + tig0043655:2066-2085 Msa1463820:three_prime_UTR 30.0%
CACAACAATACAAATGTCTT+TGG - tig0043655:2108-2127 Msa1463820:intergenic 30.0%
GTATTGTTGTGTATTATGTG+TGG + tig0043655:2117-2136 Msa1463820:three_prime_UTR 30.0%
! TTTGTTGTGTTTAAGAGTGA+AGG - tig0043655:791-810 Msa1463820:intergenic 30.0%
CTCTATAACGTAAACATAGA+TGG + tig0043655:840-859 Msa1463820:exon 30.0%
CTTACCATTGATATGTTACA+TGG - tig0043655:952-971 Msa1463820:intergenic 30.0%
!! CTATTGATTGGTAATGCAAA+AGG - tig0043655:1117-1136 Msa1463820:intergenic 30.0%
ATAGCTAAGGTTCTATTGAT+TGG - tig0043655:1129-1148 Msa1463820:intergenic 30.0%
CTCAAGGTACATATTACTTA+CGG + tig0043655:1493-1512 Msa1463820:intron 30.0%
!!! TCACCTTTTTTTCATTGAAC+AGG + tig0043655:1739-1758 Msa1463820:intron 30.0%
GAACCTGTTCAATGAAAAAA+AGG - tig0043655:1745-1764 Msa1463820:intergenic 30.0%
TTAAAACACTACTAGGTTAC+TGG + tig0043655:1958-1977 Msa1463820:exon 30.0%
ATAGGTAGTTGCAATATGTT+TGG + tig0043655:2018-2037 Msa1463820:three_prime_UTR 30.0%
AATATGTTTGGCTATATTGC+AGG + tig0043655:2030-2049 Msa1463820:three_prime_UTR 30.0%
ATATGTTTGGCTATATTGCA+GGG + tig0043655:2031-2050 Msa1463820:three_prime_UTR 30.0%
AAATTGTTTGGACCCATATT+GGG + tig0043655:2066-2085 Msa1463820:three_prime_UTR 30.0%
CACAACAATACAAATGTCTT+TGG - tig0043655:2108-2127 Msa1463820:intergenic 30.0%
GTATTGTTGTGTATTATGTG+TGG + tig0043655:2117-2136 Msa1463820:three_prime_UTR 30.0%
AGTTTCCTCACAAATAGTAG+TGG + tig0043655:994-1013 Msa1463820:CDS 35.0%
!! AGAGTACTTTTGCATTGAAG+TGG - tig0043655:1186-1205 Msa1463820:intergenic 35.0%
TTGACTTATATTGACAGGAC+CGG + tig0043655:1422-1441 Msa1463820:intron 35.0%
CGTAAGTAATATGTACCTTG+AGG - tig0043655:1495-1514 Msa1463820:intergenic 35.0%
GTACATATTACTTACGGTCA+AGG + tig0043655:1499-1518 Msa1463820:intron 35.0%
CTTCTGTAACCTTTACATTG+TGG + tig0043655:1630-1649 Msa1463820:intron 35.0%
! TAGTCTCCTACTATCTGTTT+TGG + tig0043655:1918-1937 Msa1463820:CDS 35.0%
CAATGACCAAAACAGATAGT+AGG - tig0043655:1927-1946 Msa1463820:intergenic 35.0%
!!! CTACTATCTGTTTTGGTCAT+TGG + tig0043655:1925-1944 Msa1463820:CDS 35.0%
! AGGGTTTTGCTCAAATTGTT+TGG + tig0043655:2054-2073 Msa1463820:three_prime_UTR 35.0%
CAAATTGTTTGGACCCATAT+TGG + tig0043655:2065-2084 Msa1463820:three_prime_UTR 35.0%
AATTGTTTGGACCCATATTG+GGG + tig0043655:2067-2086 Msa1463820:three_prime_UTR 35.0%
AGTTTCCTCACAAATAGTAG+TGG + tig0043655:994-1013 Msa1463820:CDS 35.0%
!! AGAGTACTTTTGCATTGAAG+TGG - tig0043655:1186-1205 Msa1463820:intergenic 35.0%
TTGACTTATATTGACAGGAC+CGG + tig0043655:1422-1441 Msa1463820:intron 35.0%
CGTAAGTAATATGTACCTTG+AGG - tig0043655:1495-1514 Msa1463820:intergenic 35.0%
GTACATATTACTTACGGTCA+AGG + tig0043655:1499-1518 Msa1463820:intron 35.0%
CTTCTGTAACCTTTACATTG+TGG + tig0043655:1630-1649 Msa1463820:intron 35.0%
! TAGTCTCCTACTATCTGTTT+TGG + tig0043655:1918-1937 Msa1463820:CDS 35.0%
CAATGACCAAAACAGATAGT+AGG - tig0043655:1927-1946 Msa1463820:intergenic 35.0%
!!! CTACTATCTGTTTTGGTCAT+TGG + tig0043655:1925-1944 Msa1463820:CDS 35.0%
! AGGGTTTTGCTCAAATTGTT+TGG + tig0043655:2054-2073 Msa1463820:three_prime_UTR 35.0%
CAAATTGTTTGGACCCATAT+TGG + tig0043655:2065-2084 Msa1463820:three_prime_UTR 35.0%
AATTGTTTGGACCCATATTG+GGG + tig0043655:2067-2086 Msa1463820:three_prime_UTR 35.0%
GACGCCATGTAACATATCAA+TGG + tig0043655:945-964 Msa1463820:CDS 40.0%
TTGTGAGGAAACTCAAGCAA+GGG - tig0043655:987-1006 Msa1463820:intergenic 40.0%
TTTGTGAGGAAACTCAAGCA+AGG - tig0043655:988-1007 Msa1463820:intergenic 40.0%
CATTGCCACTACTATTTGTG+AGG - tig0043655:1002-1021 Msa1463820:intergenic 40.0%
CTCACAAATAGTAGTGGCAA+TGG + tig0043655:1000-1019 Msa1463820:CDS 40.0%
! TGTATGTGTCTTATTGCCAC+TGG + tig0043655:1084-1103 Msa1463820:CDS 40.0%
!! TTTGTTGTTCTCTGCAGCTT+CGG + tig0043655:1302-1321 Msa1463820:intron 40.0%
! TAAGAGATGGTGTCAATGCA+GGG - tig0043655:1470-1489 Msa1463820:intergenic 40.0%
! AAGGTTCTAACTTGTAGTCG+CGG + tig0043655:1518-1537 Msa1463820:intron 40.0%
!! GATAATGGATTGGTTGTCTC+AGG - tig0043655:1811-1830 Msa1463820:intergenic 40.0%
AACCAATACCACCCCAATAT+GGG - tig0043655:2081-2100 Msa1463820:intergenic 40.0%
AAACCAATACCACCCCAATA+TGG - tig0043655:2082-2101 Msa1463820:intergenic 40.0%
GACGCCATGTAACATATCAA+TGG + tig0043655:945-964 Msa1463820:CDS 40.0%
TTGTGAGGAAACTCAAGCAA+GGG - tig0043655:987-1006 Msa1463820:intergenic 40.0%
TTTGTGAGGAAACTCAAGCA+AGG - tig0043655:988-1007 Msa1463820:intergenic 40.0%
CATTGCCACTACTATTTGTG+AGG - tig0043655:1002-1021 Msa1463820:intergenic 40.0%
CTCACAAATAGTAGTGGCAA+TGG + tig0043655:1000-1019 Msa1463820:CDS 40.0%
! TGTATGTGTCTTATTGCCAC+TGG + tig0043655:1084-1103 Msa1463820:CDS 40.0%
!! TTTGTTGTTCTCTGCAGCTT+CGG + tig0043655:1302-1321 Msa1463820:intron 40.0%
! TAAGAGATGGTGTCAATGCA+GGG - tig0043655:1470-1489 Msa1463820:intergenic 40.0%
! AAGGTTCTAACTTGTAGTCG+CGG + tig0043655:1518-1537 Msa1463820:intron 40.0%
!! GATAATGGATTGGTTGTCTC+AGG - tig0043655:1811-1830 Msa1463820:intergenic 40.0%
AACCAATACCACCCCAATAT+GGG - tig0043655:2081-2100 Msa1463820:intergenic 40.0%
AAACCAATACCACCCCAATA+TGG - tig0043655:2082-2101 Msa1463820:intergenic 40.0%
GAAACTCAAGCAAGGGCTAA+TGG - tig0043655:980-999 Msa1463820:intergenic 45.0%
ATCCTTGCTTCCACTTGTGA+GGG - tig0043655:1064-1083 Msa1463820:intergenic 45.0%
AATCCTTGCTTCCACTTGTG+AGG - tig0043655:1065-1084 Msa1463820:intergenic 45.0%
TGCAAAAGGAACATTGCCAG+TGG - tig0043655:1103-1122 Msa1463820:intergenic 45.0%
ACGAGGAAGAGAGATAGCTA+AGG - tig0043655:1142-1161 Msa1463820:intergenic 45.0%
TTGCATTGAAGTGGAACACC+AGG - tig0043655:1177-1196 Msa1463820:intergenic 45.0%
! TTGTTGTTCTCTGCAGCTTC+GGG + tig0043655:1303-1322 Msa1463820:intron 45.0%
!! CATAATAGAGTGTTGCGTCG+CGG - tig0043655:1354-1373 Msa1463820:intergenic 45.0%
! AAGAGATGGTGTCAATGCAG+GGG - tig0043655:1469-1488 Msa1463820:intergenic 45.0%
! CTAAGAGATGGTGTCAATGC+AGG - tig0043655:1471-1490 Msa1463820:intergenic 45.0%
ACACCATCTCTTAGTCCTCA+AGG + tig0043655:1477-1496 Msa1463820:intron 45.0%
GTACCTTGAGGACTAAGAGA+TGG - tig0043655:1483-1502 Msa1463820:intergenic 45.0%
! TTTTGATGCGGCCACAATTG+TGG + tig0043655:1587-1606 Msa1463820:intron 45.0%
!! CGCTGATGGAGATAATGGAT+TGG - tig0043655:1821-1840 Msa1463820:intergenic 45.0%
GCGTTGCAGATGGAATATCA+GGG - tig0043655:1846-1865 Msa1463820:intergenic 45.0%
! ATGATGGAGTCAGATCAGAG+CGG - tig0043655:1879-1898 Msa1463820:intergenic 45.0%
GATGATGATCCAGCAGATGA+TGG - tig0043655:1895-1914 Msa1463820:intergenic 45.0%
TGTTTGGCTATATTGCAGGG+AGG + tig0043655:2034-2053 Msa1463820:three_prime_UTR 45.0%
GTTTGGCTATATTGCAGGGA+GGG + tig0043655:2035-2054 Msa1463820:three_prime_UTR 45.0%
GAAACTCAAGCAAGGGCTAA+TGG - tig0043655:980-999 Msa1463820:intergenic 45.0%
ATCCTTGCTTCCACTTGTGA+GGG - tig0043655:1064-1083 Msa1463820:intergenic 45.0%
AATCCTTGCTTCCACTTGTG+AGG - tig0043655:1065-1084 Msa1463820:intergenic 45.0%
TGCAAAAGGAACATTGCCAG+TGG - tig0043655:1103-1122 Msa1463820:intergenic 45.0%
ACGAGGAAGAGAGATAGCTA+AGG - tig0043655:1142-1161 Msa1463820:intergenic 45.0%
TTGCATTGAAGTGGAACACC+AGG - tig0043655:1177-1196 Msa1463820:intergenic 45.0%
! TTGTTGTTCTCTGCAGCTTC+GGG + tig0043655:1303-1322 Msa1463820:intron 45.0%
!! CATAATAGAGTGTTGCGTCG+CGG - tig0043655:1354-1373 Msa1463820:intergenic 45.0%
! AAGAGATGGTGTCAATGCAG+GGG - tig0043655:1469-1488 Msa1463820:intergenic 45.0%
! CTAAGAGATGGTGTCAATGC+AGG - tig0043655:1471-1490 Msa1463820:intergenic 45.0%
ACACCATCTCTTAGTCCTCA+AGG + tig0043655:1477-1496 Msa1463820:intron 45.0%
GTACCTTGAGGACTAAGAGA+TGG - tig0043655:1483-1502 Msa1463820:intergenic 45.0%
! TTTTGATGCGGCCACAATTG+TGG + tig0043655:1587-1606 Msa1463820:intron 45.0%
!! CGCTGATGGAGATAATGGAT+TGG - tig0043655:1821-1840 Msa1463820:intergenic 45.0%
GCGTTGCAGATGGAATATCA+GGG - tig0043655:1846-1865 Msa1463820:intergenic 45.0%
! ATGATGGAGTCAGATCAGAG+CGG - tig0043655:1879-1898 Msa1463820:intergenic 45.0%
GATGATGATCCAGCAGATGA+TGG - tig0043655:1895-1914 Msa1463820:intergenic 45.0%
TGTTTGGCTATATTGCAGGG+AGG + tig0043655:2034-2053 Msa1463820:three_prime_UTR 45.0%
GTTTGGCTATATTGCAGGGA+GGG + tig0043655:2035-2054 Msa1463820:three_prime_UTR 45.0%
GCATTGCAGCAATCAGCAGT+GGG - tig0043655:1033-1052 Msa1463820:intergenic 50.0%
TGCATTGCAGCAATCAGCAG+TGG - tig0043655:1034-1053 Msa1463820:intergenic 50.0%
GCAATCAAGACCCTCACAAG+TGG + tig0043655:1051-1070 Msa1463820:CDS 50.0%
GTCAATGCAGGGGAAAGAGA+TGG - tig0043655:1459-1478 Msa1463820:intergenic 50.0%
CTAGAGCTCGACCACAATTG+TGG - tig0043655:1601-1620 Msa1463820:intergenic 50.0%
CTACTGCGGCCACAATGTAA+AGG - tig0043655:1642-1661 Msa1463820:intergenic 50.0%
!! AGAGAAGGTGTCACTGGTGA+GGG - tig0043655:1778-1797 Msa1463820:intergenic 50.0%
GGGTTCGCTGATGGAGATAA+TGG - tig0043655:1826-1845 Msa1463820:intergenic 50.0%
GGAATATCAGGGTTCGCTGA+TGG - tig0043655:1835-1854 Msa1463820:intergenic 50.0%
GGCGTTGCAGATGGAATATC+AGG - tig0043655:1847-1866 Msa1463820:intergenic 50.0%
GATATTCCATCTGCAACGCC+CGG + tig0043655:1847-1866 Msa1463820:CDS 50.0%
GATCTGACTCCATCATCTGC+TGG + tig0043655:1883-1902 Msa1463820:CDS 50.0%
TGTTTGGACCCATATTGGGG+TGG + tig0043655:2070-2089 Msa1463820:three_prime_UTR 50.0%
GACCCATATTGGGGTGGTAT+TGG + tig0043655:2076-2095 Msa1463820:three_prime_UTR 50.0%
GCATTGCAGCAATCAGCAGT+GGG - tig0043655:1033-1052 Msa1463820:intergenic 50.0%
TGCATTGCAGCAATCAGCAG+TGG - tig0043655:1034-1053 Msa1463820:intergenic 50.0%
GCAATCAAGACCCTCACAAG+TGG + tig0043655:1051-1070 Msa1463820:CDS 50.0%
GTCAATGCAGGGGAAAGAGA+TGG - tig0043655:1459-1478 Msa1463820:intergenic 50.0%
CTAGAGCTCGACCACAATTG+TGG - tig0043655:1601-1620 Msa1463820:intergenic 50.0%
CTACTGCGGCCACAATGTAA+AGG - tig0043655:1642-1661 Msa1463820:intergenic 50.0%
!! AGAGAAGGTGTCACTGGTGA+GGG - tig0043655:1778-1797 Msa1463820:intergenic 50.0%
GGGTTCGCTGATGGAGATAA+TGG - tig0043655:1826-1845 Msa1463820:intergenic 50.0%
GGAATATCAGGGTTCGCTGA+TGG - tig0043655:1835-1854 Msa1463820:intergenic 50.0%
GGCGTTGCAGATGGAATATC+AGG - tig0043655:1847-1866 Msa1463820:intergenic 50.0%
GATATTCCATCTGCAACGCC+CGG + tig0043655:1847-1866 Msa1463820:CDS 50.0%
GATCTGACTCCATCATCTGC+TGG + tig0043655:1883-1902 Msa1463820:CDS 50.0%
TGTTTGGACCCATATTGGGG+TGG + tig0043655:2070-2089 Msa1463820:three_prime_UTR 50.0%
GACCCATATTGGGGTGGTAT+TGG + tig0043655:2076-2095 Msa1463820:three_prime_UTR 50.0%
GTCTCTCTACCGCCTAACTG+TGG + tig0043655:873-892 Msa1463820:CDS 55.0%
CGGCTAACACCACAGTTAGG+CGG - tig0043655:885-904 Msa1463820:intergenic 55.0%
CCACGGCTAACACCACAGTT+AGG - tig0043655:888-907 Msa1463820:intergenic 55.0%
! CCTAACTGTGGTGTTAGCCG+TGG + tig0043655:885-904 Msa1463820:CDS 55.0%
GTTAGCCGTGGCTTCGATCA+TGG + tig0043655:897-916 Msa1463820:CDS 55.0%
TGCAGCCATGATCGAAGCCA+CGG - tig0043655:905-924 Msa1463820:intergenic 55.0%
GTCGTGATTTGAGCGTAGGC+TGG - tig0043655:928-947 Msa1463820:intergenic 55.0%
TGGCGTCGTGATTTGAGCGT+AGG - tig0043655:932-951 Msa1463820:intergenic 55.0%
GACCCTCACAAGTGGAAGCA+AGG + tig0043655:1059-1078 Msa1463820:CDS 55.0%
CCAGGCAAGTTACAAGCACG+AGG - tig0043655:1159-1178 Msa1463820:intergenic 55.0%
! CCTCGTGCTTGTAACTTGCC+TGG + tig0043655:1156-1175 Msa1463820:CDS 55.0%
GGTTTACCTGGAGCAGGAAG+CGG - tig0043655:1333-1352 Msa1463820:intergenic 55.0%
TTGACAGGACCGGCGTCTTT+CGG + tig0043655:1432-1451 Msa1463820:intron 55.0%
! CTAACTTGTAGTCGCGGTCG+TGG + tig0043655:1524-1543 Msa1463820:intron 55.0%
GCGGTCAGCAACTACTACTG+CGG - tig0043655:1656-1675 Msa1463820:intergenic 55.0%
!! CAGAGAAGGTGTCACTGGTG+AGG - tig0043655:1779-1798 Msa1463820:intergenic 55.0%
!! GGTGACAGAGAAGGTGTCAC+TGG - tig0043655:1784-1803 Msa1463820:intergenic 55.0%
TCAGGCTGTGGTGACAGAGA+AGG - tig0043655:1793-1812 Msa1463820:intergenic 55.0%
!! GGATTGGTTGTCTCAGGCTG+TGG - tig0043655:1805-1824 Msa1463820:intergenic 55.0%
GTCTCTCTACCGCCTAACTG+TGG + tig0043655:873-892 Msa1463820:CDS 55.0%
CGGCTAACACCACAGTTAGG+CGG - tig0043655:885-904 Msa1463820:intergenic 55.0%
CCACGGCTAACACCACAGTT+AGG - tig0043655:888-907 Msa1463820:intergenic 55.0%
! CCTAACTGTGGTGTTAGCCG+TGG + tig0043655:885-904 Msa1463820:CDS 55.0%
GTTAGCCGTGGCTTCGATCA+TGG + tig0043655:897-916 Msa1463820:CDS 55.0%
TGCAGCCATGATCGAAGCCA+CGG - tig0043655:905-924 Msa1463820:intergenic 55.0%
GTCGTGATTTGAGCGTAGGC+TGG - tig0043655:928-947 Msa1463820:intergenic 55.0%
TGGCGTCGTGATTTGAGCGT+AGG - tig0043655:932-951 Msa1463820:intergenic 55.0%
GACCCTCACAAGTGGAAGCA+AGG + tig0043655:1059-1078 Msa1463820:CDS 55.0%
CCAGGCAAGTTACAAGCACG+AGG - tig0043655:1159-1178 Msa1463820:intergenic 55.0%
! CCTCGTGCTTGTAACTTGCC+TGG + tig0043655:1156-1175 Msa1463820:CDS 55.0%
GGTTTACCTGGAGCAGGAAG+CGG - tig0043655:1333-1352 Msa1463820:intergenic 55.0%
TTGACAGGACCGGCGTCTTT+CGG + tig0043655:1432-1451 Msa1463820:intron 55.0%
! CTAACTTGTAGTCGCGGTCG+TGG + tig0043655:1524-1543 Msa1463820:intron 55.0%
GCGGTCAGCAACTACTACTG+CGG - tig0043655:1656-1675 Msa1463820:intergenic 55.0%
!! CAGAGAAGGTGTCACTGGTG+AGG - tig0043655:1779-1798 Msa1463820:intergenic 55.0%
!! GGTGACAGAGAAGGTGTCAC+TGG - tig0043655:1784-1803 Msa1463820:intergenic 55.0%
TCAGGCTGTGGTGACAGAGA+AGG - tig0043655:1793-1812 Msa1463820:intergenic 55.0%
!! GGATTGGTTGTCTCAGGCTG+TGG - tig0043655:1805-1824 Msa1463820:intergenic 55.0%
GCAGCAATCAGCAGTGGGTG+AGG - tig0043655:1028-1047 Msa1463820:intergenic 60.0%
GTGTTGCGTCGCGGTTTACC+TGG - tig0043655:1345-1364 Msa1463820:intergenic 60.0%
TGACAGGACCGGCGTCTTTC+GGG + tig0043655:1433-1452 Msa1463820:intron 60.0%
AGAGATGGCCCGAAAGACGC+CGG - tig0043655:1444-1463 Msa1463820:intergenic 60.0%
AGATCAGAGCGGCCACTTCC+GGG - tig0043655:1868-1887 Msa1463820:intergenic 60.0%
CAGATCAGAGCGGCCACTTC+CGG - tig0043655:1869-1888 Msa1463820:intergenic 60.0%
GCAGCAATCAGCAGTGGGTG+AGG - tig0043655:1028-1047 Msa1463820:intergenic 60.0%
GTGTTGCGTCGCGGTTTACC+TGG - tig0043655:1345-1364 Msa1463820:intergenic 60.0%
TGACAGGACCGGCGTCTTTC+GGG + tig0043655:1433-1452 Msa1463820:intron 60.0%
AGAGATGGCCCGAAAGACGC+CGG - tig0043655:1444-1463 Msa1463820:intergenic 60.0%
AGATCAGAGCGGCCACTTCC+GGG - tig0043655:1868-1887 Msa1463820:intergenic 60.0%
CAGATCAGAGCGGCCACTTC+CGG - tig0043655:1869-1888 Msa1463820:intergenic 60.0%
CGTCGCGGTTTACCTGGAGC+AGG - tig0043655:1339-1358 Msa1463820:intergenic 65.0%
! CCACTTCCGGGCGTTGCAGA+TGG - tig0043655:1856-1875 Msa1463820:intergenic 65.0%
CCATCTGCAACGCCCGGAAG+TGG + tig0043655:1853-1872 Msa1463820:CDS 65.0%
CGTCGCGGTTTACCTGGAGC+AGG - tig0043655:1339-1358 Msa1463820:intergenic 65.0%
! CCACTTCCGGGCGTTGCAGA+TGG - tig0043655:1856-1875 Msa1463820:intergenic 65.0%
CCATCTGCAACGCCCGGAAG+TGG + tig0043655:1853-1872 Msa1463820:CDS 65.0%
GGCTCACCGCTTCCTGCTCC+AGG + tig0043655:1324-1343 Msa1463820:CDS 70.0%
GGCTCACCGCTTCCTGCTCC+AGG + tig0043655:1324-1343 Msa1463820:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0043655 gene 767 2166 767 ID=Msa1463820;Name=Msa1463820
tig0043655 mRNA 767 2166 767 ID=Msa1463820-mRNA-1;Parent=Msa1463820;Name=Msa1463820-mRNA-1;_AED=0.12;_eAED=0.12;_QI=92|0.5|1|1|1|1|3|194|192
tig0043655 exon 767 1198 767 ID=Msa1463820-mRNA-1:exon:24198;Parent=Msa1463820-mRNA-1
tig0043655 exon 1319 1345 1319 ID=Msa1463820-mRNA-1:exon:24199;Parent=Msa1463820-mRNA-1
tig0043655 exon 1761 2166 1761 ID=Msa1463820-mRNA-1:exon:24200;Parent=Msa1463820-mRNA-1
tig0043655 five_prime_UTR 767 858 767 ID=Msa1463820-mRNA-1:five_prime_utr;Parent=Msa1463820-mRNA-1
tig0043655 CDS 859 1198 859 ID=Msa1463820-mRNA-1:cds;Parent=Msa1463820-mRNA-1
tig0043655 CDS 1319 1345 1319 ID=Msa1463820-mRNA-1:cds;Parent=Msa1463820-mRNA-1
tig0043655 CDS 1761 1972 1761 ID=Msa1463820-mRNA-1:cds;Parent=Msa1463820-mRNA-1
tig0043655 three_prime_UTR 1973 2166 1973 ID=Msa1463820-mRNA-1:three_prime_utr;Parent=Msa1463820-mRNA-1
Gene Sequence

>Msa1463820

ATGGATCATTTCGCGTCTCTCTACCGCCTAACTGTGGTGTTAGCCGTGGCTTCGATCATGGCTGCACCAGCCTACGCTCAAATCACGACGCCATGTAACATATCAATGGTAAGTAGTACCATTAGCCCTTGCTTGAGTTTCCTCACAAATAGTAGTGGCAATGGAACCTCACCCACTGCTGATTGCTGCAATGCAATCAAGACCCTCACAAGTGGAAGCAAGGATTGTATGTGTCTTATTGCCACTGGCAATGTTCCTTTTGCATTACCAATCAATAGAACCTTAGCTATCTCTCTTCCTCGTGCTTGTAACTTGCCTGGTGTTCCACTTCAATGCAAAACTTCGGGCTCACCGCTTCCTGCTCCAGGTTCTTCTATACTTCCCTCACCAGTGACACCTTCTCTGTCACCACAGCCTGAGACAACCAATCCATTATCTCCATCAGCGAACCCTGATATTCCATCTGCAACGCCCGGAAGTGGCCGCTCTGATCTGACTCCATCATCTGCTGGATCATCATCTTATAGTCTCCTACTATCTGTTTTGGTCATTGGATTTTCTATATTAAAACACTACTAG
Protein sequence

>Msa1463820

MDHFASLYRLTVVLAVASIMAAPAYAQITTPCNISMVSSTISPCLSFLTNSSGNGTSPTADCCNAIKTLTSGSKDCMCLIATGNVPFALPINRTLAISLPRACNLPGVPLQCKTSGSPLPAPGSSILPSPVTPSLSPQPETTNPLSPSANPDIPSATPGSGRSDLTPSSAGSSSYSLLLSVLVIGFSILKHY*