AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0738570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0738570 MtrunA17_Chr5g0438021 95.625 160 7 0 1 160 1 160 8.07e-111 318
Msa0738570 MtrunA17_Chr3g0129051 70.513 156 42 1 5 160 6 157 1.40e-73 224
Msa0738570 MtrunA17_Chr3g0129061 75.641 156 34 2 5 160 6 157 3.72e-73 223
Msa0738570 MtrunA17_Chr3g0129041 75.000 156 35 2 5 160 6 157 1.01e-72 221
Msa0738570 MtrunA17_Chr3g0106921 69.014 142 44 0 18 159 12 153 1.60e-69 213
Msa0738570 MtrunA17_Chr5g0432441 62.745 153 57 0 6 158 5 157 7.19e-63 196
Msa0738570 MtrunA17_Chr2g0320061 61.745 149 56 1 12 160 7 154 8.22e-63 196
Msa0738570 MtrunA17_Chr2g0320071 64.430 149 49 1 12 160 10 154 1.19e-62 196
Msa0738570 MtrunA17_Chr3g0114451 64.516 155 53 1 8 160 1 155 1.41e-62 195
Msa0738570 MtrunA17_Chr2g0320101 60.625 160 57 2 1 160 1 154 1.78e-62 195
Msa0738570 MtrunA17_Chr2g0320051 62.416 149 55 1 12 160 7 154 1.85e-62 195
Msa0738570 MtrunA17_Chr7g0244451 67.407 135 44 0 24 158 17 151 5.10e-61 191
Msa0738570 MtrunA17_Chr2g0320091 59.375 160 59 2 1 160 1 154 4.61e-60 189
Msa0738570 MtrunA17_Chr4g0038151 61.268 142 55 0 19 160 13 154 3.26e-59 187
Msa0738570 MtrunA17_Chr2g0320031 57.047 149 64 0 12 160 7 155 1.26e-58 185
Msa0738570 MtrunA17_Chr7g0244461 60.714 140 55 0 19 158 8 147 2.17e-58 184
Msa0738570 MtrunA17_Chr5g0432401 59.748 159 60 3 6 160 3 161 3.77e-57 181
Msa0738570 MtrunA17_Chr4g0043391 57.051 156 63 2 7 160 3 156 7.31e-57 181
Msa0738570 MtrunA17_Chr4g0038161 57.931 145 59 1 16 160 11 153 2.13e-56 179
Msa0738570 MtrunA17_Chr4g0013171 58.784 148 59 2 16 161 11 158 5.50e-56 179
Msa0738570 MtrunA17_Chr1g0213061 60.839 143 55 1 18 159 13 155 2.87e-55 177
Msa0738570 MtrunA17_Chr4g0013181 66.406 128 42 1 34 160 62 189 3.54e-55 177
Msa0738570 MtrunA17_Chr4g0038171 58.333 144 58 1 17 160 12 153 4.77e-55 176
Msa0738570 MtrunA17_Chr5g0432331 58.491 159 62 3 6 160 30 188 9.17e-54 174
Msa0738570 MtrunA17_Chr4g0013201 54.305 151 68 1 10 159 24 174 1.41e-53 173
Msa0738570 MtrunA17_Chr4g0068621 52.410 166 67 1 7 160 2 167 6.05e-53 171
Msa0738570 MtrunA17_Chr5g0404721 63.200 125 46 0 31 155 30 154 6.98e-53 171
Msa0738570 MtrunA17_Chr3g0106901 61.333 150 57 1 11 160 12 160 2.41e-52 169
Msa0738570 MtrunA17_Chr4g0070951 63.359 131 46 1 29 159 26 154 5.81e-52 169
Msa0738570 MtrunA17_Chr7g0224371 53.503 157 68 3 7 158 2 158 5.97e-52 168
Msa0738570 MtrunA17_Chr2g0299691 55.556 153 63 2 13 162 35 185 1.78e-51 168
Msa0738570 MtrunA17_Chr5g0432351 63.704 135 46 2 29 160 23 157 2.37e-51 167
Msa0738570 MtrunA17_Chr8g0372601 62.400 125 47 0 31 155 30 154 5.62e-51 166
Msa0738570 MtrunA17_Chr1g0175561 53.548 155 68 2 7 160 3 154 1.95e-50 164
Msa0738570 MtrunA17_Chr5g0432341 56.688 157 64 3 8 160 1 157 2.31e-50 164
Msa0738570 MtrunA17_Chr5g0402571 52.564 156 72 1 5 160 3 156 6.34e-50 163
Msa0738570 MtrunA17_Chr3g0114491 70.370 108 32 0 31 138 18 125 3.95e-49 155
Msa0738570 MtrunA17_Chr7g0258811 52.055 146 69 1 16 160 54 199 5.37e-49 162
Msa0738570 MtrunA17_Chr5g0402581 56.818 132 57 0 29 160 25 156 9.55e-49 160
Msa0738570 MtrunA17_Chr1g0155611 56.250 128 56 0 31 158 46 173 1.52e-48 160
Msa0738570 MtrunA17_Chr4g0013191 56.000 125 55 0 35 159 50 174 2.48e-48 159
Msa0738570 MtrunA17_Chr5g0400331 59.200 125 51 0 31 155 26 150 7.29e-47 155
Msa0738570 MtrunA17_Chr4g0013821 54.198 131 60 0 30 160 27 157 7.38e-47 155
Msa0738570 MtrunA17_Chr3g0107881 56.296 135 56 2 27 158 23 157 2.49e-46 154
Msa0738570 MtrunA17_Chr4g0013721 51.449 138 67 0 23 160 92 229 4.86e-46 155
Msa0738570 MtrunA17_Chr2g0292241 49.645 141 71 0 26 166 24 164 4.15e-45 151
Msa0738570 MtrunA17_Chr2g0292211 49.286 140 71 0 27 166 36 175 1.11e-44 150
Msa0738570 MtrunA17_Chr2g0292271 47.403 154 78 1 16 166 11 164 3.66e-44 149
Msa0738570 MtrunA17_Chr2g0292151 51.064 141 69 0 26 166 24 164 6.05e-44 148
Msa0738570 MtrunA17_Chr2g0292091 47.097 155 80 1 12 166 7 159 7.13e-44 148
Msa0738570 MtrunA17_Chr2g0292101 47.297 148 78 0 12 159 6 153 4.34e-43 146
Msa0738570 MtrunA17_Chr4g0024121 50.694 144 66 2 12 155 12 150 1.01e-42 144
Msa0738570 MtrunA17_Chr2g0292181 44.311 167 89 2 1 166 10 173 1.40e-42 145
Msa0738570 MtrunA17_Chr6g0477071 45.695 151 81 1 6 156 29 178 1.66e-42 144
Msa0738570 MtrunA17_Chr2g0291751 45.181 166 89 1 1 166 1 164 6.12e-42 143
Msa0738570 MtrunA17_Chr2g0292111 47.518 141 74 0 26 166 24 164 1.56e-41 142
Msa0738570 MtrunA17_Chr2g0292261 46.875 160 81 2 1 159 1 157 1.61e-41 142
Msa0738570 MtrunA17_Chr2g0292251 46.099 141 76 0 26 166 23 163 3.51e-41 141
Msa0738570 MtrunA17_Chr5g0421411 58.333 108 45 0 51 158 1 108 4.29e-41 139
Msa0738570 MtrunA17_Chr2g0292291 46.667 135 72 0 26 160 25 159 3.07e-40 139
Msa0738570 MtrunA17_Chr1g0157051 44.898 147 80 1 8 154 1 146 1.95e-39 136
Msa0738570 MtrunA17_Chr4g0063671 47.297 148 73 3 12 158 1 144 2.22e-39 135
Msa0738570 MtrunA17_Chr2g0322861 45.283 159 84 3 2 159 57 213 7.44e-39 136
Msa0738570 MtrunA17_Chr5g0405991 44.898 147 80 1 12 158 4 149 1.33e-37 131
Msa0738570 MtrunA17_Chr1g0204011 44.218 147 76 2 12 158 13 153 5.42e-37 129
Msa0738570 MtrunA17_Chr2g0316191 50.388 129 61 2 29 155 74 201 9.30e-37 131
Msa0738570 MtrunA17_Chr1g0188431 49.206 126 63 1 31 156 39 163 9.79e-37 129
Msa0738570 MtrunA17_Chr3g0128061 46.528 144 74 1 16 156 8 151 1.02e-36 129
Msa0738570 MtrunA17_Chr8g0371381 46.565 131 69 1 26 156 21 150 6.33e-36 127
Msa0738570 MtrunA17_Chr2g0316811 47.727 132 66 2 29 158 48 178 7.87e-36 127
Msa0738570 MtrunA17_Chr2g0316481 49.612 129 62 2 29 155 74 201 8.14e-36 128
Msa0738570 MtrunA17_Chr2g0316791 49.612 129 62 2 29 155 74 201 1.09e-35 128
Msa0738570 MtrunA17_Chr7g0254201 45.522 134 72 1 27 160 16 148 1.69e-35 125
Msa0738570 MtrunA17_Chr6g0460711 42.177 147 84 1 13 159 3 148 2.83e-35 125
Msa0738570 MtrunA17_Chr2g0316831 48.837 129 63 2 29 155 110 237 3.77e-35 127
Msa0738570 MtrunA17_Chr7g0268501 41.290 155 87 3 8 161 1 152 1.95e-34 123
Msa0738570 MtrunA17_Chr7g0268521 41.290 155 87 3 8 161 1 152 1.95e-34 123
Msa0738570 MtrunA17_Chr2g0296621 47.656 128 63 2 31 158 50 173 3.40e-34 123
Msa0738570 MtrunA17_Chr2g0279041 43.537 147 76 3 12 158 11 150 5.24e-34 122
Msa0738570 MtrunA17_Chr2g0278671 42.466 146 81 2 24 169 17 159 5.60e-34 122
Msa0738570 MtrunA17_Chr1g0197411 42.177 147 83 2 14 158 11 157 2.18e-33 120
Msa0738570 MtrunA17_Chr7g0254231 44.882 127 69 1 29 155 20 145 2.28e-33 120
Msa0738570 MtrunA17_Chr4g0075241 42.000 150 83 1 13 158 7 156 4.61e-33 119
Msa0738570 MtrunA17_Chr3g0136751 39.456 147 85 1 9 155 3 145 6.44e-33 119
Msa0738570 MtrunA17_Chr7g0225221 42.446 139 78 2 18 155 12 149 6.64e-32 116
Msa0738570 MtrunA17_Chr4g0076211 42.143 140 71 4 35 169 45 179 1.29e-31 116
Msa0738570 MtrunA17_Chr1g0194271 40.385 156 88 2 8 158 4 159 3.43e-31 115
Msa0738570 MtrunA17_Chr1g0194151 40.385 156 88 2 8 158 4 159 5.59e-31 114
Msa0738570 MtrunA17_Chr8g0364291 43.972 141 78 1 16 155 8 148 5.61e-31 114
Msa0738570 MtrunA17_Chr4g0046131 46.154 130 67 2 30 156 41 170 9.72e-30 111
Msa0738570 MtrunA17_Chr6g0467251 39.623 159 84 3 7 155 5 161 1.14e-29 111
Msa0738570 MtrunA17_Chr1g0181511 40.909 132 75 2 29 159 25 154 1.43e-29 110
Msa0738570 MtrunA17_Chr2g0311241 50.000 110 53 2 29 138 58 165 2.06e-29 111
Msa0738570 MtrunA17_Chr1g0181481 40.972 144 80 3 29 168 25 167 5.91e-29 108
Msa0738570 MtrunA17_Chr1g0181431 40.441 136 79 2 29 163 25 159 8.89e-29 108
Msa0738570 MtrunA17_Chr1g0164891 46.457 127 65 2 35 158 93 219 9.28e-29 109
Msa0738570 MtrunA17_Chr5g0401431 40.000 160 89 4 13 165 6 165 1.84e-28 107
Msa0738570 MtrunA17_Chr6g0451571 38.298 141 85 2 20 158 23 163 2.01e-28 107
Msa0738570 MtrunA17_Chr4g0074851 39.189 148 87 2 13 158 12 158 3.00e-28 107
Msa0738570 MtrunA17_Chr2g0329641 40.667 150 80 4 12 155 11 157 6.08e-28 106
Msa0738570 MtrunA17_Chr2g0311251 45.802 131 63 4 29 155 9 135 2.25e-27 105
Msa0738570 MtrunA17_Chr1g0148021 39.873 158 90 4 12 166 9 164 2.54e-27 104
Msa0738570 MtrunA17_Chr4g0053871 40.769 130 74 2 29 155 25 154 2.21e-25 99.4
Msa0738570 MtrunA17_Chr4g0072091 40.152 132 71 3 31 158 24 151 7.67e-25 97.8
Msa0738570 MtrunA17_Chr6g0467161 36.628 172 93 4 1 159 5 173 2.65e-24 96.7
Msa0738570 MtrunA17_Chr6g0467121 39.370 127 71 3 31 155 74 196 3.05e-24 97.1
Msa0738570 MtrunA17_Chr1g0181491 38.938 113 67 2 29 141 10 120 9.27e-22 85.9
Msa0738570 MtrunA17_Chr2g0279061 52.381 84 36 2 75 158 12 91 2.77e-20 84.7
Msa0738570 MtrunA17_Chr6g0468731 55.556 72 28 2 70 141 22 89 5.92e-20 79.7
Msa0738570 MtrunA17_Chr5g0413051 38.406 138 72 4 25 155 67 198 7.33e-19 82.4
Msa0738570 MtrunA17_Chr2g0316141 50.617 81 37 2 77 155 11 90 1.03e-18 80.9
Msa0738570 MtrunA17_Chr2g0316181 50.617 81 37 2 77 155 11 90 1.03e-18 80.9
Msa0738570 MtrunA17_Chr1g0196191 57.576 66 27 1 73 138 27 91 3.53e-16 71.6
Msa0738570 MtrunA17_Chr7g0254181 48.333 60 31 0 103 162 31 90 1.36e-13 64.7
Msa0738570 MtrunA17_Chr7g0254171 37.500 72 44 1 77 148 4 74 2.22e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0738570 AT5G05340.1 75.000 132 33 0 29 160 28 159 1.59e-70 216
Msa0738570 AT5G58400.1 67.832 143 45 1 18 159 16 158 2.99e-63 197
Msa0738570 AT5G58390.1 64.865 148 52 0 12 159 2 149 5.42e-63 197
Msa0738570 AT5G06720.1 55.034 149 65 1 13 159 12 160 8.61e-55 176
Msa0738570 AT5G06730.1 52.174 161 75 1 1 159 1 161 7.16e-53 172
Msa0738570 AT5G19890.1 59.060 149 56 2 12 160 13 156 1.04e-51 168
Msa0738570 AT4G36430.1 55.172 145 64 1 14 158 14 157 1.95e-51 167
Msa0738570 AT1G44970.1 57.463 134 57 0 25 158 39 172 2.09e-51 168
Msa0738570 AT4G16270.1 61.600 125 48 0 36 160 55 179 4.61e-51 167
Msa0738570 AT5G66390.1 53.797 158 70 2 13 169 14 169 5.75e-51 166
Msa0738570 AT2G18140.1 55.396 139 62 0 23 161 27 165 6.16e-51 166
Msa0738570 AT1G49570.1 59.091 132 54 0 29 160 46 177 1.43e-50 166
Msa0738570 AT3G50990.1 57.353 136 58 0 26 161 36 171 3.79e-50 164
Msa0738570 AT1G14550.1 53.741 147 66 1 12 158 9 153 1.07e-49 163
Msa0738570 AT1G68850.1 46.497 157 79 2 4 159 6 158 1.74e-49 162
Msa0738570 AT2G18150.1 55.970 134 59 0 25 158 30 163 1.79e-49 162
Msa0738570 AT2G38380.1 50.649 154 72 1 5 158 9 158 6.60e-49 161
Msa0738570 AT4G08770.1 55.224 134 60 0 22 155 15 148 9.97e-49 161
Msa0738570 AT2G38390.1 48.052 154 76 1 5 158 9 158 1.75e-48 160
Msa0738570 AT1G14540.1 52.518 139 66 0 27 165 17 155 2.88e-48 159
Msa0738570 AT3G32980.1 54.610 141 62 1 18 158 20 158 3.98e-48 159
Msa0738570 AT4G08780.1 56.589 129 56 0 27 155 20 148 4.93e-48 159
Msa0738570 AT2G22420.1 48.701 154 76 1 7 160 2 152 1.31e-46 155
Msa0738570 AT2G35380.1 52.318 151 67 1 14 159 8 158 8.22e-46 153
Msa0738570 AT3G49120.1 52.381 147 69 1 12 158 14 159 9.39e-46 153
Msa0738570 AT3G49110.1 50.000 146 66 1 20 158 15 160 3.11e-45 152
Msa0738570 AT3G01190.1 49.669 151 74 2 9 159 4 152 1.08e-43 147
Msa0738570 AT5G19880.1 51.351 148 69 2 14 158 7 154 3.49e-43 146
Msa0738570 AT3G21770.1 46.324 136 71 2 26 161 24 157 5.51e-39 135
Msa0738570 AT1G05260.1 47.059 153 75 4 11 159 2 152 7.43e-39 135
Msa0738570 AT4G11290.1 47.945 146 71 3 18 159 9 153 1.27e-38 134
Msa0738570 AT5G42180.1 44.304 158 85 3 8 165 1 155 3.48e-38 133
Msa0738570 AT1G05240.1 48.413 126 63 2 31 156 26 149 9.47e-38 132
Msa0738570 AT1G05250.1 48.413 126 63 2 31 156 26 149 9.47e-38 132
Msa0738570 AT2G41480.1 48.630 146 70 3 14 158 11 152 5.65e-37 130
Msa0738570 AT5G15180.1 42.500 160 89 3 1 159 1 158 1.29e-36 129
Msa0738570 AT5G17820.1 46.575 146 73 3 17 161 9 150 2.24e-36 128
Msa0738570 AT5G64120.1 43.506 154 76 4 12 158 9 158 4.16e-36 128
Msa0738570 AT3G03670.1 45.070 142 77 1 18 159 10 150 5.76e-36 127
Msa0738570 AT5G17820.2 46.575 146 73 3 17 161 62 203 8.76e-36 128
Msa0738570 AT4G31760.1 46.043 139 71 1 18 156 16 150 9.25e-36 127
Msa0738570 AT4G25980.2 43.662 142 77 2 19 160 59 197 1.25e-35 125
Msa0738570 AT5G51890.1 43.624 149 83 1 7 155 2 149 2.08e-35 126
Msa0738570 AT4G33420.1 48.413 126 64 1 31 156 37 161 2.70e-35 125
Msa0738570 AT5G14130.1 43.056 144 80 2 12 154 13 155 5.46e-35 125
Msa0738570 AT4G33420.2 48.413 126 64 1 31 156 84 208 6.40e-35 125
Msa0738570 AT5G14130.2 42.759 145 81 2 12 155 54 197 1.04e-34 125
Msa0738570 AT4G25980.1 43.972 141 76 2 19 159 14 151 1.12e-34 124
Msa0738570 AT2G24800.2 46.923 130 65 1 29 158 29 154 3.40e-34 120
Msa0738570 AT5G39580.2 47.581 124 61 2 35 158 29 148 2.48e-33 118
Msa0738570 AT5G40150.1 41.045 134 78 1 26 158 27 160 4.38e-33 120
Msa0738570 AT1G30870.1 45.070 142 69 2 31 167 51 188 4.73e-33 120
Msa0738570 AT4G33870.1 43.750 128 71 1 31 158 68 194 5.22e-33 121
Msa0738570 AT2G24800.1 46.923 130 65 1 29 158 29 154 6.74e-33 119
Msa0738570 AT5G39580.1 47.581 124 61 2 35 158 29 148 7.99e-33 119
Msa0738570 AT5G64100.1 48.000 125 61 2 35 159 41 161 1.07e-32 119
Msa0738570 AT5G47000.1 44.218 147 78 3 12 155 13 158 1.35e-32 119
Msa0738570 AT4G26010.1 43.284 134 75 1 26 159 17 149 3.61e-32 117
Msa0738570 AT5G64110.1 40.351 171 87 4 10 169 5 171 4.54e-32 117
Msa0738570 AT3G28200.1 39.552 134 80 1 26 158 16 149 5.34e-32 117
Msa0738570 AT4G26010.2 43.284 134 75 1 26 159 45 177 5.70e-32 117
Msa0738570 AT4G17690.1 43.750 128 71 1 29 155 24 151 7.86e-32 117
Msa0738570 AT1G77100.1 45.255 137 73 2 23 159 16 150 9.99e-32 116
Msa0738570 AT1G71695.1 42.063 126 72 1 31 155 44 169 1.60e-31 116
Msa0738570 AT1G34510.1 41.912 136 78 1 24 159 15 149 6.01e-30 111
Msa0738570 AT1G24110.1 41.667 156 84 5 16 165 4 158 7.90e-30 111
Msa0738570 AT2G39040.1 43.382 136 74 3 25 158 40 174 1.47e-29 111
Msa0738570 AT2G34060.1 46.099 141 69 5 27 161 38 177 7.36e-29 109
Msa0738570 AT4G37530.2 41.912 136 76 2 23 155 19 154 8.77e-29 107
Msa0738570 AT4G21960.1 39.850 133 79 1 23 155 23 154 1.82e-28 108
Msa0738570 AT5G22410.1 42.857 147 77 5 19 162 15 157 2.01e-28 108
Msa0738570 AT2G43480.1 40.426 141 78 3 22 160 26 162 2.02e-28 108
Msa0738570 AT4G37530.1 41.176 136 77 2 23 155 19 154 2.93e-28 107
Msa0738570 AT3G49960.1 40.816 147 84 2 12 155 8 154 7.28e-28 106
Msa0738570 AT5G24070.1 43.750 128 65 3 35 159 38 161 8.82e-28 106
Msa0738570 AT4G37520.1 40.769 130 74 2 29 155 25 154 1.96e-27 105
Msa0738570 AT5G67400.1 41.333 150 77 3 16 154 4 153 4.21e-27 104
Msa0738570 AT2G37130.1 39.695 131 77 2 27 156 26 155 8.12e-27 103
Msa0738570 AT2G18980.1 41.259 143 77 3 17 156 11 149 1.17e-26 103
Msa0738570 AT2G37130.2 40.323 124 72 2 34 156 2 124 9.19e-26 100
Msa0738570 AT4G37520.2 40.000 130 72 3 29 155 25 151 3.02e-25 99.4
Msa0738570 AT3G17070.1 35.948 153 94 1 7 155 10 162 1.13e-24 98.2
Msa0738570 AT4G30170.1 38.971 136 74 3 29 160 25 155 1.05e-23 95.5
Msa0738570 AT2G35380.2 63.636 66 24 0 93 158 4 69 4.29e-22 89.7
Msa0738570 AT3G17070.2 37.349 83 51 1 73 155 18 99 3.82e-11 60.5

Find 36 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTACAATTAGCCGTTTA+AGG 0.161616 5_1:-56339794 None:intergenic
TTCACAGTGTCAAAGAGTTT+TGG 0.311726 5_1:+56342307 None:intergenic
CAAGTTTCACATCCCATGTT+GGG 0.349153 5_1:+56340379 None:intergenic
TGACTCTTTGAATCCTTAAA+CGG 0.401571 5_1:+56339781 None:intergenic
TGCCAATCCCAACAGAAATT+CGG 0.407832 5_1:-56340746 Msa0738570:CDS
CATGCGGGTCTCCTTTGATA+TGG 0.427995 5_1:+56342267 None:intergenic
CCACGGGCCGAATTTCTGTT+GGG 0.448742 5_1:+56340739 None:intergenic
CCAAGTTTCACATCCCATGT+TGG 0.448886 5_1:+56340378 None:intergenic
GGCCGAATTTCTGTTGGGAT+TGG 0.459419 5_1:+56340744 None:intergenic
ATCTTTCGAAAGATTAGCAT+TGG 0.480051 5_1:+56342348 None:intergenic
GCTTAAATTTCCCAGCTTGG+AGG 0.480636 5_1:-56340402 Msa0738570:intron
CCAACATGGGATGTGAAACT+TGG 0.494973 5_1:-56340378 Msa0738570:CDS
TCCACGGGCCGAATTTCTGT+TGG 0.507509 5_1:+56340738 None:intergenic
CCCAGCTTGGAGGCCCAACA+TGG 0.520178 5_1:-56340392 Msa0738570:intron
GTCGATCACTTCGAATCCAC+GGG 0.532124 5_1:+56340723 None:intergenic
GATGACACATCAAGCTTCAC+CGG 0.536161 5_1:-56340778 Msa0738570:CDS
AATTGATACAAGAACCAAAA+GGG 0.548391 5_1:+56342378 None:intergenic
ATAACATTGCAGGGATGTGA+TGG 0.563333 5_1:-56340814 Msa0738570:intron
AACAGAGTCTCTAGCAGTGA+TGG 0.565223 5_1:+56340639 None:intergenic
TAGAAGAGAAGCACCCATGC+GGG 0.569776 5_1:+56342252 None:intergenic
TGTCGATCACTTCGAATCCA+CGG 0.573405 5_1:+56340722 None:intergenic
CAATTGATACAAGAACCAAA+AGG 0.573924 5_1:+56342377 None:intergenic
CATATCAAAGGAGACCCGCA+TGG 0.578197 5_1:-56342266 Msa0738570:CDS
CAGATCAAGTTGTAGAGGGA+AGG 0.578669 5_1:-56339823 Msa0738570:CDS
CCCAACAGAAATTCGGCCCG+TGG 0.584922 5_1:-56340739 Msa0738570:CDS
GCAGTGGAGAAAGTATGTCC+AGG 0.603574 5_1:-56340688 Msa0738570:CDS
AGTTCAATCTGCCATATCAA+AGG 0.604429 5_1:-56342278 Msa0738570:CDS
TCAGCACATGAAACTGCACC+TGG 0.605024 5_1:+56340670 None:intergenic
CCAGCTTGGAGGCCCAACAT+GGG 0.607919 5_1:-56340391 Msa0738570:intron
ATATTCAGATCAAGTTGTAG+AGG 0.608283 5_1:-56339828 Msa0738570:intron
CGACAAAATCAAATCAGCAG+TGG 0.609985 5_1:-56340704 Msa0738570:CDS
AGTCTCTAGCAGTGATGGCG+AGG 0.612615 5_1:+56340644 None:intergenic
GTACATTAACAAAGCAATCG+TGG 0.614906 5_1:+56342218 None:intergenic
TATTCAGATCAAGTTGTAGA+GGG 0.619848 5_1:-56339827 Msa0738570:intron
ATATCAAAGGAGACCCGCAT+GGG 0.639894 5_1:-56342265 Msa0738570:CDS
GTAGAAGAGAAGCACCCATG+CGG 0.717536 5_1:+56342251 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATATA+TGG - chr5_1:56340308-56340327 Msa0738570:intron 0.0%
!! ATATATATATATATATATAT+GGG - chr5_1:56340084-56340103 Msa0738570:intron 0.0%
!!! TTTATTTTGTCATATTTATA+AGG + chr5_1:56340867-56340886 None:intergenic 10.0%
!! TTAAAAAAAAAGTTAAAAGT+AGG + chr5_1:56340933-56340952 None:intergenic 10.0%
!! ATTTAGTGTTATATTGTATT+GGG + chr5_1:56341070-56341089 None:intergenic 15.0%
!! TATTTAGTGTTATATTGTAT+TGG + chr5_1:56341071-56341090 None:intergenic 15.0%
!!! TATTTAACAATATTGACTTA+TGG - chr5_1:56341211-56341230 Msa0738570:intron 15.0%
!! TAAAAAATTAATGTGATCAT+AGG - chr5_1:56341315-56341334 Msa0738570:intron 15.0%
!!! TATTTTTAATATAACATTGC+AGG - chr5_1:56341399-56341418 Msa0738570:intron 15.0%
!!! ATTTTTAATATAACATTGCA+GGG - chr5_1:56341400-56341419 Msa0738570:intron 15.0%
!!! TTTATTCTCTAAAATGTATA+GGG + chr5_1:56341691-56341710 None:intergenic 15.0%
!! TATATATATATATCTGTGTG+TGG - chr5_1:56340091-56340110 Msa0738570:intron 20.0%
!! AAATAGTTAAATACACCTTA+AGG + chr5_1:56340409-56340428 None:intergenic 20.0%
!!! GACACTTTTTTTTAGAAAAT+AGG + chr5_1:56341001-56341020 None:intergenic 20.0%
!! GTTGTATTGAAAATTGAAAT+GGG + chr5_1:56341037-56341056 None:intergenic 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG + chr5_1:56341038-56341057 None:intergenic 20.0%
!! AAAAGTGGAATATATTGTAT+TGG + chr5_1:56341112-56341131 None:intergenic 20.0%
!!! AAGAAAAGTGTATAAATGAA+GGG + chr5_1:56341135-56341154 None:intergenic 20.0%
!!! TAAGAAAAGTGTATAAATGA+AGG + chr5_1:56341136-56341155 None:intergenic 20.0%
!!! TGATTATTATGATGCAATTT+TGG + chr5_1:56341627-56341646 None:intergenic 20.0%
!!! CTTTATTCTCTAAAATGTAT+AGG + chr5_1:56341692-56341711 None:intergenic 20.0%
!! TTTATATTTGTATTCACTCA+CGG + chr5_1:56341771-56341790 None:intergenic 20.0%
!!! TTCTCTTTTTGAATCTATTA+TGG - chr5_1:56342257-56342276 Msa0738570:CDS 20.0%
!!! ATTGATTTGTTTTTCCCTTT+TGG - chr5_1:56339831-56339850 Msa0738570:intron 25.0%
! AATTGATACAAGAACCAAAA+GGG + chr5_1:56339848-56339867 None:intergenic 25.0%
!!! TTTTGGTTCTTGTATCAATT+GGG - chr5_1:56339848-56339867 Msa0738570:intron 25.0%
!!! CACAGTTGACAAATTTTAAT+TGG - chr5_1:56340159-56340178 Msa0738570:intron 25.0%
!! ACACCTTCTAAAAAATAAGT+GGG - chr5_1:56340451-56340470 Msa0738570:intron 25.0%
! ACACCTTCAAAAAAATAAGT+GGG + chr5_1:56340516-56340535 None:intergenic 25.0%
! AAGTTGTTAAATACACCTTA+AGG + chr5_1:56340555-56340574 None:intergenic 25.0%
! ACACCTTAATAAAAACTAGT+GGG - chr5_1:56340598-56340617 Msa0738570:intron 25.0%
! AATTAATGTGATCATAGGAT+TGG - chr5_1:56341320-56341339 Msa0738570:intron 25.0%
!! TTATTATGATGCAATTTTGG+TGG + chr5_1:56341624-56341643 None:intergenic 25.0%
! NNNNNNNNCTTGAGATGAGT+TGG + chr5_1:56341943-56341962 None:intergenic 25.0%
! AAAAAGAGAAAATACTTGTG+TGG + chr5_1:56342247-56342266 None:intergenic 25.0%
CAATTGATACAAGAACCAAA+AGG + chr5_1:56339849-56339868 None:intergenic 30.0%
!!! CTTTTGGTTCTTGTATCAAT+TGG - chr5_1:56339847-56339866 Msa0738570:intron 30.0%
ATCTTTCGAAAGATTAGCAT+TGG + chr5_1:56339878-56339897 None:intergenic 30.0%
! ACACCCACTAATTTTTATGT+GGG - chr5_1:56340364-56340383 Msa0738570:CDS 30.0%
ACTCCCACATAAAAATTAGT+GGG + chr5_1:56340370-56340389 None:intergenic 30.0%
! TTTTAGCAAACTACACCTTA+AGG - chr5_1:56340391-56340410 Msa0738570:intron 30.0%
! CACACCTTCTAAAAAATAAG+TGG - chr5_1:56340450-56340469 Msa0738570:intron 30.0%
!!! ACACCCACTTATTTTTTAGA+AGG + chr5_1:56340457-56340476 None:intergenic 30.0%
!!! ACACCCACTTATTTTTTTGA+AGG - chr5_1:56340510-56340529 Msa0738570:intron 30.0%
CACACCTTCAAAAAAATAAG+TGG + chr5_1:56340517-56340536 None:intergenic 30.0%
! TTTTAGCAACATTCACCTTA+AGG - chr5_1:56340537-56340556 Msa0738570:intron 30.0%
CACACCTTAATAAAAACTAG+TGG - chr5_1:56340597-56340616 Msa0738570:intron 30.0%
!!! ACACCCACTAGTTTTTATTA+AGG + chr5_1:56340604-56340623 None:intergenic 30.0%
TAGACATACACATACATACA+GGG + chr5_1:56340639-56340658 None:intergenic 30.0%
ATAGACATACACATACATAC+AGG + chr5_1:56340640-56340659 None:intergenic 30.0%
AAAGAATGATTTCCCACTAA+CGG - chr5_1:56340898-56340917 Msa0738570:intron 30.0%
AGAAAATAGGTCAGTTATTC+CGG + chr5_1:56340988-56341007 None:intergenic 30.0%
!! TGAAATGGGTCAGTTATTTT+GGG + chr5_1:56341023-56341042 None:intergenic 30.0%
! TTGAAATGGGTCAGTTATTT+TGG + chr5_1:56341024-56341043 None:intergenic 30.0%
TGTATAAATGAAGGGAAAAG+TGG + chr5_1:56341127-56341146 None:intergenic 30.0%
TCATCCTAATTAAACTAGCA+TGG + chr5_1:56341260-56341279 None:intergenic 30.0%
GGAGCAAAAATATAAATGAG+AGG - chr5_1:56342278-56342297 Msa0738570:CDS 30.0%
! ATATTCAGATCAAGTTGTAG+AGG - chr5_1:56342395-56342414 Msa0738570:CDS 30.0%
! TATTCAGATCAAGTTGTAGA+GGG - chr5_1:56342396-56342415 Msa0738570:CDS 30.0%
! TTCACAGTGTCAAAGAGTTT+TGG + chr5_1:56339919-56339938 None:intergenic 35.0%
AGTTCAATCTGCCATATCAA+AGG - chr5_1:56339945-56339964 Msa0738570:intron 35.0%
GTACATTAACAAAGCAATCG+TGG + chr5_1:56340008-56340027 None:intergenic 35.0%
! CACACCCACTAATTTTTATG+TGG - chr5_1:56340363-56340382 Msa0738570:CDS 35.0%
CACTCCCACATAAAAATTAG+TGG + chr5_1:56340371-56340390 None:intergenic 35.0%
TGTATGTTAGCAACTCCTAT+AGG + chr5_1:56340493-56340512 None:intergenic 35.0%
AGACATACACATACATACAG+GGG + chr5_1:56340638-56340657 None:intergenic 35.0%
TAAACAAAAGTCGATGCATG+TGG - chr5_1:56340772-56340791 Msa0738570:CDS 35.0%
GAAAATAGGTCAGTTATTCC+GGG + chr5_1:56340987-56341006 None:intergenic 35.0%
TCTGTGTGAAAAACCAAAGT+AGG - chr5_1:56341157-56341176 Msa0738570:intron 35.0%
CCAAAGTAGGTCAATTATAC+CGG - chr5_1:56341170-56341189 Msa0738570:intron 35.0%
CAAAGTAGGTCAATTATACC+GGG - chr5_1:56341171-56341190 Msa0738570:intron 35.0%
TGACTTATGGTTTCTTTGTC+TGG - chr5_1:56341224-56341243 Msa0738570:intron 35.0%
! GATGCCATGCTAGTTTAATT+AGG - chr5_1:56341253-56341272 Msa0738570:intron 35.0%
AAAAGCAAGTAACATCGAAG+AGG + chr5_1:56341292-56341311 None:intergenic 35.0%
TATGCTTAAATTTCCCAGCT+TGG - chr5_1:56341818-56341837 Msa0738570:intron 35.0%
AGTAAATCAGCTGCAAACAA+TGG - chr5_1:56341890-56341909 Msa0738570:intron 35.0%
TTTGAAGAGAAAGACTTGTG+TGG - chr5_1:56342070-56342089 Msa0738570:intron 35.0%
AAGAGAGAAGAGAGAGAAAA+TGG + chr5_1:56342166-56342185 None:intergenic 35.0%
TCTCTTCTCTCTCATTTCTT+TGG - chr5_1:56342189-56342208 Msa0738570:intron 35.0%
TCATTTCTTTGGTTGTGACT+AGG - chr5_1:56342200-56342219 Msa0738570:intron 35.0%
TTTGGTTGTGACTAGGAAAT+GGG - chr5_1:56342207-56342226 Msa0738570:intron 35.0%
GGAAGTCTTAGTTAGTTAGT+AGG - chr5_1:56342299-56342318 Msa0738570:CDS 35.0%
! TCTATTCTAGCTACTACATC+CGG - chr5_1:56342341-56342360 Msa0738570:CDS 35.0%
TGTGGCTGAAAAATAGGAAG+TGG + chr5_1:56340198-56340217 None:intergenic 40.0%
CTATTTGCCACAATTCAAGG+AGG + chr5_1:56340221-56340240 None:intergenic 40.0%
GCACTATTTGCCACAATTCA+AGG + chr5_1:56340224-56340243 None:intergenic 40.0%
GGCAAATAGTGCATTGTGAT+TGG - chr5_1:56340232-56340251 Msa0738570:intron 40.0%
GTATCCTAGCAAATGCCTAT+AGG - chr5_1:56340475-56340494 Msa0738570:intron 40.0%
AACTCCTATAGGCATTTGCT+AGG + chr5_1:56340482-56340501 None:intergenic 40.0%
ATGATTTCCCACTAACGGTT+TGG - chr5_1:56340903-56340922 Msa0738570:intron 40.0%
AGGTAAACCAAACCGTTAGT+GGG + chr5_1:56340913-56340932 None:intergenic 40.0%
TAGGTAAACCAAACCGTTAG+TGG + chr5_1:56340914-56340933 None:intergenic 40.0%
CCGGTATAATTGACCTACTT+TGG + chr5_1:56341173-56341192 None:intergenic 40.0%
ATAACATTGCAGGGATGTGA+TGG - chr5_1:56341409-56341428 Msa0738570:intron 40.0%
TGCCAATCCCAACAGAAATT+CGG - chr5_1:56341477-56341496 Msa0738570:intron 40.0%
CGACAAAATCAAATCAGCAG+TGG - chr5_1:56341519-56341538 Msa0738570:intron 40.0%
CAAGTTTCACATCCCATGTT+GGG + chr5_1:56341847-56341866 None:intergenic 40.0%
!! NNCTTGAGATGAGTTGGTTG+AGG + chr5_1:56341937-56341956 None:intergenic 40.0%
TGGATCAAGACCACACAATT+GGG - chr5_1:56342090-56342109 Msa0738570:intron 40.0%
CTTTGGTTGTGACTAGGAAA+TGG - chr5_1:56342206-56342225 Msa0738570:intron 40.0%
TTGGTTGTGACTAGGAAATG+GGG - chr5_1:56342208-56342227 Msa0738570:intron 40.0%
GAAAATACTTGTGTGGACCA+GGG + chr5_1:56342240-56342259 None:intergenic 40.0%
AGAAAATACTTGTGTGGACC+AGG + chr5_1:56342241-56342260 None:intergenic 40.0%
GTCGTTTATGTAGTAGCATC+CGG + chr5_1:56342363-56342382 None:intergenic 40.0%
ATATCAAAGGAGACCCGCAT+GGG - chr5_1:56339958-56339977 Msa0738570:intron 45.0%
! AGGAGGTGTGGCTGAAAAAT+AGG + chr5_1:56340204-56340223 None:intergenic 45.0%
! CATACATACAGGGGTTTGCT+AGG + chr5_1:56340629-56340648 None:intergenic 45.0%
ATAGGTCAGTTATTCCGGGA+CGG + chr5_1:56340983-56341002 None:intergenic 45.0%
GTAGGTCAATTATACCGGGA+CGG - chr5_1:56341175-56341194 Msa0738570:intron 45.0%
AAATTATACTCCCTCCGTCC+CGG + chr5_1:56341192-56341211 None:intergenic 45.0%
GATGACACATCAAGCTTCAC+CGG - chr5_1:56341445-56341464 Msa0738570:intron 45.0%
TGTCGATCACTTCGAATCCA+CGG + chr5_1:56341504-56341523 None:intergenic 45.0%
AACAGAGTCTCTAGCAGTGA+TGG + chr5_1:56341587-56341606 None:intergenic 45.0%
GCTTAAATTTCCCAGCTTGG+AGG - chr5_1:56341821-56341840 Msa0738570:intron 45.0%
CCAAGTTTCACATCCCATGT+TGG + chr5_1:56341848-56341867 None:intergenic 45.0%
CCAACATGGGATGTGAAACT+TGG - chr5_1:56341845-56341864 Msa0738570:intron 45.0%
AGTTGGTTGAGGCTTGAAGT+GGG + chr5_1:56341926-56341945 None:intergenic 45.0%
GTGGATCAAGACCACACAAT+TGG - chr5_1:56342089-56342108 Msa0738570:intron 45.0%
CAAGACCACACAATTGGGTT+TGG - chr5_1:56342095-56342114 Msa0738570:intron 45.0%
TGTGACCAAACCCAATTGTG+TGG + chr5_1:56342103-56342122 None:intergenic 45.0%
TGTGACTAGGAAATGGGGTT+TGG - chr5_1:56342213-56342232 Msa0738570:intron 45.0%
! CAGATCAAGTTGTAGAGGGA+AGG - chr5_1:56342400-56342419 Msa0738570:CDS 45.0%
CATGCGGGTCTCCTTTGATA+TGG + chr5_1:56339959-56339978 None:intergenic 50.0%
CATATCAAAGGAGACCCGCA+TGG - chr5_1:56339957-56339976 Msa0738570:intron 50.0%
!! TAGAAGAGAAGCACCCATGC+GGG + chr5_1:56339974-56339993 None:intergenic 50.0%
!! GTAGAAGAGAAGCACCCATG+CGG + chr5_1:56339975-56339994 None:intergenic 50.0%
AGCCACACCTCCTTGAATTG+TGG - chr5_1:56340211-56340230 Msa0738570:intron 50.0%
TGCCACAATTCAAGGAGGTG+TGG + chr5_1:56340216-56340235 None:intergenic 50.0%
TCTCAATACTACCTCCGTCC+CGG - chr5_1:56340966-56340985 Msa0738570:intron 50.0%
GTCAATTATACCGGGACGGA+GGG - chr5_1:56341179-56341198 Msa0738570:intron 50.0%
!! GGGATTGGCAGTTTTCTCTC+CGG + chr5_1:56341467-56341486 None:intergenic 50.0%
GGCCGAATTTCTGTTGGGAT+TGG + chr5_1:56341482-56341501 None:intergenic 50.0%
GTCGATCACTTCGAATCCAC+GGG + chr5_1:56341503-56341522 None:intergenic 50.0%
! GCAGTGGAGAAAGTATGTCC+AGG - chr5_1:56341535-56341554 Msa0738570:intron 50.0%
TCAGCACATGAAACTGCACC+TGG + chr5_1:56341556-56341575 None:intergenic 50.0%
! GAGTTGGTTGAGGCTTGAAG+TGG + chr5_1:56341927-56341946 None:intergenic 50.0%
GGTCAATTATACCGGGACGG+AGG - chr5_1:56341178-56341197 Msa0738570:intron 55.0%
CCACGGGCCGAATTTCTGTT+GGG + chr5_1:56341487-56341506 None:intergenic 55.0%
TCCACGGGCCGAATTTCTGT+TGG + chr5_1:56341488-56341507 None:intergenic 55.0%
! AGTCTCTAGCAGTGATGGCG+AGG + chr5_1:56341582-56341601 None:intergenic 55.0%
TGAGGCTTGAAGTGGGTGCT+GGG + chr5_1:56341919-56341938 None:intergenic 55.0%
TTGAGGCTTGAAGTGGGTGC+TGG + chr5_1:56341920-56341939 None:intergenic 55.0%
! AGGAAATGGGGTTTGGTCCC+TGG - chr5_1:56342220-56342239 Msa0738570:intron 55.0%
GGTCAGTTATTCCGGGACGG+AGG + chr5_1:56340980-56340999 None:intergenic 60.0%
CCCAACAGAAATTCGGCCCG+TGG - chr5_1:56341484-56341503 Msa0738570:intron 60.0%
CCATGTTGGGCCTCCAAGCT+GGG + chr5_1:56341834-56341853 None:intergenic 60.0%
CCAGCTTGGAGGCCCAACAT+GGG - chr5_1:56341832-56341851 Msa0738570:intron 60.0%
CCCAGCTTGGAGGCCCAACA+TGG - chr5_1:56341831-56341850 Msa0738570:intron 65.0%
CCCATGTTGGGCCTCCAAGC+TGG + chr5_1:56341835-56341854 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 56339796 56342449 56339796 ID=Msa0738570;Name=Msa0738570
chr5_1 mRNA 56339796 56342449 56339796 ID=Msa0738570-mRNA-1;Parent=Msa0738570;Name=Msa0738570-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0|0|0.75|1|1|4|0|173
chr5_1 exon 56342222 56342449 56342222 ID=Msa0738570-mRNA-1:exon:15985;Parent=Msa0738570-mRNA-1
chr5_1 exon 56340633 56340824 56340633 ID=Msa0738570-mRNA-1:exon:15984;Parent=Msa0738570-mRNA-1
chr5_1 exon 56340355 56340409 56340355 ID=Msa0738570-mRNA-1:exon:15983;Parent=Msa0738570-mRNA-1
chr5_1 exon 56339796 56339842 56339796 ID=Msa0738570-mRNA-1:exon:15982;Parent=Msa0738570-mRNA-1
chr5_1 CDS 56342222 56342449 56342222 ID=Msa0738570-mRNA-1:cds;Parent=Msa0738570-mRNA-1
chr5_1 CDS 56340633 56340824 56340633 ID=Msa0738570-mRNA-1:cds;Parent=Msa0738570-mRNA-1
chr5_1 CDS 56340355 56340409 56340355 ID=Msa0738570-mRNA-1:cds;Parent=Msa0738570-mRNA-1
chr5_1 CDS 56339796 56339842 56339796 ID=Msa0738570-mRNA-1:cds;Parent=Msa0738570-mRNA-1
Gene Sequence

>Msa0738570

ATGGCTTCGTTTTGTTCTAGCATGACAAGTAATTTATTGATTTGTTTTTCCCTTTTGGTTCTTGTATCAATTGGGAGTGCCAATGCTAATCTTTCGAAAGATTATTACTATAGTTCTTGTCCAAAACTCTTTGACACTGTGAAGTGTGAAGTTCAATCTGCCATATCAAAGGAGACCCGCATGGGTGCTTCTCTTCTACGTTTGTTCTTCCACGATTGCTTTGTTAATGGATGTGATGGTTCAATTCTTCTCGATGACACATCAAGCTTCACCGGAGAGAAAACTGCCAATCCCAACAGAAATTCGGCCCGTGGATTCGAAGTGATCGACAAAATCAAATCAGCAGTGGAGAAAGTATGTCCAGGTGCAGTTTCATGTGCTGATATCCTCGCCATCACTGCTAGAGACTCTGTTGAGATCCTTGGAGGCCCAACATGGGATGTGAAACTTGGAAGAAGAGATGCTAGAACAGCTAATCAAGTTGTAGAGGGAAGGCACGATGATTTACAATTAGCCGTTTAA
Protein sequence

>Msa0738570

MASFCSSMTSNLLICFSLLVLVSIGSANANLSKDYYYSSCPKLFDTVKCEVQSAISKETRMGASLLRLFFHDCFVNGCDGSILLDDTSSFTGEKTANPNRNSARGFEVIDKIKSAVEKVCPGAVSCADILAITARDSVEILGGPTWDVKLGRRDARTANQVVEGRHDDLQLAV*