AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0629040


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0629040 MtrunA17_Chr4g0005111 97.382 191 5 0 1 191 1 191 5.09e-140 388
Msa0629040 MtrunA17_Chr4g0005091 66.842 190 48 3 3 191 1 176 6.30e-86 250
Msa0629040 MtrunA17_Chr4g0005131 66.477 176 57 1 17 190 16 191 1.37e-82 243
Msa0629040 MtrunA17_Chr4g0005071 60.989 182 66 3 14 190 15 196 1.45e-75 225
Msa0629040 MtrunA17_Chr4g0005081 60.656 183 66 3 14 190 15 197 2.27e-74 222
Msa0629040 MtrunA17_Chr4g0005031 60.870 184 64 4 15 190 38 221 2.20e-72 218
Msa0629040 MtrunA17_Chr7g0243501 59.043 188 74 1 6 190 5 192 6.06e-72 216
Msa0629040 MtrunA17_Chr4g0005041 56.831 183 71 4 15 190 17 198 2.39e-69 209
Msa0629040 MtrunA17_Chr4g0005011 55.435 184 72 5 15 190 16 197 2.57e-65 199
Msa0629040 MtrunA17_Chr1g0175911 46.486 185 94 3 9 190 10 192 7.82e-57 177
Msa0629040 MtrunA17_Chr7g0259071 41.989 181 99 4 13 190 14 191 1.43e-46 151
Msa0629040 MtrunA17_Chr4g0037481 45.098 153 82 1 40 190 40 192 2.03e-46 151
Msa0629040 MtrunA17_Chr7g0259091 39.894 188 110 3 6 190 7 194 6.16e-46 150
Msa0629040 MtrunA17_Chr7g0259101 41.489 188 107 3 6 190 7 194 9.18e-45 152
Msa0629040 MtrunA17_Chr7g0259101 40.571 175 101 3 19 190 219 393 4.02e-42 145
Msa0629040 MtrunA17_Chr1g0177051 43.709 151 84 1 40 189 35 185 3.04e-42 140
Msa0629040 MtrunA17_Chr7g0259121 41.358 162 77 3 41 190 38 193 1.71e-39 134
Msa0629040 MtrunA17_Chr3g0085321 38.095 189 106 4 7 189 5 188 2.21e-39 133
Msa0629040 MtrunA17_Chr5g0446751 37.234 188 107 5 9 190 7 189 4.39e-38 129
Msa0629040 MtrunA17_Chr4g0040551 36.313 179 112 2 14 191 9 186 4.59e-38 129
Msa0629040 MtrunA17_Chr3g0090231 36.667 180 112 2 13 191 8 186 5.17e-38 129
Msa0629040 MtrunA17_Chr3g0136641 41.558 154 83 3 42 190 37 188 9.30e-38 129
Msa0629040 MtrunA17_Chr3g0085291 38.776 147 87 1 43 189 43 186 5.16e-37 127
Msa0629040 MtrunA17_Chr3g0085311 36.364 187 108 4 9 189 7 188 1.16e-36 126
Msa0629040 MtrunA17_Chr3g0136631 37.097 186 108 5 11 190 7 189 1.38e-36 125
Msa0629040 MtrunA17_Chr3g0085301 38.462 156 95 1 34 189 39 193 7.09e-35 121
Msa0629040 MtrunA17_Chr4g0068391 34.211 152 99 1 40 190 29 180 3.25e-25 96.3
Msa0629040 MtrunA17_Chr4g0068381 34.615 156 98 2 36 190 28 180 1.53e-24 94.7
Msa0629040 MtrunA17_Chr4g0024951 31.618 136 92 1 42 176 29 164 5.38e-16 72.4
Msa0629040 MtrunA17_Chr4g0024961 30.496 141 92 2 46 185 33 168 2.11e-15 70.5
Msa0629040 MtrunA17_Chr1g0169281 38.462 91 55 1 77 167 141 230 6.04e-14 68.2
Msa0629040 MtrunA17_Chr1g0169291 39.560 91 54 1 77 167 135 224 2.23e-13 66.6
Msa0629040 MtrunA17_Chr1g0169511 40.000 90 53 1 78 167 136 224 2.27e-13 66.6
Msa0629040 MtrunA17_Chr1g0212861 36.975 119 68 4 78 189 235 353 2.79e-12 64.3
Msa0629040 MtrunA17_Chr8g0387711 32.353 136 68 5 50 169 37 164 1.43e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0629040 AT1G55210.1 51.872 187 85 3 7 190 3 187 1.09e-63 195
Msa0629040 AT1G55210.2 51.872 187 85 3 7 190 3 187 1.09e-63 195
Msa0629040 AT1G58170.1 48.421 190 91 3 3 190 1 185 2.03e-63 194
Msa0629040 AT3G13650.1 53.801 171 76 2 21 190 18 186 4.14e-61 188
Msa0629040 AT5G49040.1 47.727 176 89 2 18 190 16 191 1.37e-55 174
Msa0629040 AT1G65870.1 45.695 151 81 1 40 189 39 189 2.64e-46 151
Msa0629040 AT5G42500.1 47.945 146 71 3 46 189 43 185 1.55e-44 146
Msa0629040 AT3G13660.1 59.829 117 47 0 74 190 9 125 2.40e-44 144
Msa0629040 AT5G42510.1 46.000 150 78 2 41 189 35 182 5.41e-44 145
Msa0629040 AT5G49030.3 45.614 171 88 3 18 185 16 184 6.30e-43 153
Msa0629040 AT3G13662.1 47.445 137 70 1 41 177 38 172 1.49e-41 138
Msa0629040 AT1G22900.1 44.667 150 80 2 41 189 46 193 5.37e-40 135
Msa0629040 AT2G21100.1 40.940 149 87 1 42 189 39 187 7.09e-39 132
Msa0629040 AT2G21100.2 40.940 149 87 1 42 189 39 187 7.09e-39 132
Msa0629040 AT3G58090.1 47.706 109 57 0 81 189 163 271 4.49e-34 122
Msa0629040 AT2G21110.1 37.748 151 89 1 45 190 36 186 3.53e-32 114
Msa0629040 AT4G38700.1 36.842 190 107 6 8 190 7 190 5.54e-31 112
Msa0629040 AT4G23690.1 32.847 137 86 4 37 169 32 166 1.33e-15 71.6
Msa0629040 AT4G11210.1 28.994 169 108 6 5 169 2 162 1.28e-13 66.2
Msa0629040 AT4G11190.1 30.370 135 88 3 39 169 30 162 2.07e-13 65.9
Msa0629040 AT4G11180.1 33.094 139 86 5 36 169 28 164 5.44e-12 62.0

Find 38 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCTTCTTCTGAACAATTT+AGG 0.169887 4_3:-9850097 None:intergenic
ACCTCAATTGAGCTCAAAAT+TGG 0.260472 4_3:+9850264 Msa0629040:CDS
TGGAAGCACAATTACTATCT+TGG 0.342636 4_3:+9850381 Msa0629040:CDS
TTATTTGAAGGGAAATATAA+TGG 0.360119 4_3:+9850361 Msa0629040:CDS
TTAGCCACTTTAGATTCTAT+TGG 0.363260 4_3:+9850125 Msa0629040:CDS
AAGCTCAGTTTGTGCAGTAT+AGG 0.391171 4_3:-9850311 None:intergenic
ATCTATTGAGCCCACGAAAC+CGG 0.426314 4_3:-9850074 None:intergenic
GGAAGCACAATTACTATCTT+GGG 0.437728 4_3:+9850382 Msa0629040:CDS
GGGTGTAACAAGAGAGGAGA+AGG 0.466269 4_3:-9850024 None:intergenic
ATGCAAAATTCATTATCATA+AGG 0.474182 4_3:-9850339 None:intergenic
AAGAAATGATGCTTATGAAA+AGG 0.476165 4_3:+9850405 Msa0629040:CDS
AAATTGGTTGGAAGGGCTCA+AGG 0.505137 4_3:+9850280 Msa0629040:CDS
ATGCCTGTGATTGGTGGAAG+TGG 0.512180 4_3:+9850436 Msa0629040:CDS
ATAGACAACGCTTTGACCTC+AGG 0.512795 4_3:+9850241 Msa0629040:CDS
TGAGCTCAAAATTGGTTGGA+AGG 0.519356 4_3:+9850272 Msa0629040:CDS
CAATTGAGCTCAAAATTGGT+TGG 0.529085 4_3:+9850268 Msa0629040:CDS
GTTGTGTTAAACTTCGGAAG+AGG 0.543099 4_3:-9850196 None:intergenic
CATGCCAATAGAATCTAAAG+TGG 0.545838 4_3:-9850129 None:intergenic
ACAAGATGAGACCGGTTTCG+TGG 0.547240 4_3:+9850063 Msa0629040:CDS
GTTAGAGAAATGCCTGTGAT+TGG 0.553503 4_3:+9850427 Msa0629040:CDS
GTGTTAAACTTCGGAAGAGG+TGG 0.556530 4_3:-9850193 None:intergenic
AAGTTTAACACAACTAACTT+CGG 0.561635 4_3:+9850205 Msa0629040:CDS
AAGTTAGTTGTGTTAAACTT+CGG 0.577434 4_3:-9850202 None:intergenic
GAGCTCAAAATTGGTTGGAA+GGG 0.580534 4_3:+9850273 Msa0629040:CDS
CAAGATGAGACCGGTTTCGT+GGG 0.584549 4_3:+9850064 Msa0629040:CDS
AGTCCACTTCCACCAATCAC+AGG 0.592137 4_3:-9850439 None:intergenic
TGAGCTCAATTGAGGTCCTG+AGG 0.592935 4_3:-9850257 None:intergenic
AGAGAAATGCCTGTGATTGG+TGG 0.594056 4_3:+9850430 Msa0629040:CDS
AATTGGTTGGAAGGGCTCAA+GGG 0.595873 4_3:+9850281 Msa0629040:CDS
CTTGTGCTGTGACGATTGTG+AGG 0.604169 4_3:-9850045 None:intergenic
AGTTGTAGCATCTCCTGTAG+TGG 0.622414 4_3:-9850512 None:intergenic
GTCACAGCACAAGATGAGAC+CGG 0.639527 4_3:+9850055 Msa0629040:CDS
TTGTGAGGGTGTAACAAGAG+AGG 0.652546 4_3:-9850030 None:intergenic
TATTGGCATGACGTAGTCAG+CGG 0.668999 4_3:+9850142 Msa0629040:CDS
AGGTGGAACAATTTCTATCG+CGG 0.671876 4_3:-9850176 None:intergenic
TAACACAACTAACTTCGGTG+AGG 0.681754 4_3:+9850210 Msa0629040:CDS
TGGAACAATTTCTATCGCGG+TGG 0.688468 4_3:-9850173 None:intergenic
TTGTGCTGTGACGATTGTGA+GGG 0.694834 4_3:-9850044 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGCAAAATTCATTATCATA+AGG - chr4_3:9850342-9850361 None:intergenic 20.0%
!!! ATGAATTTTGCATTATTTGA+AGG + chr4_3:9850349-9850368 Msa0629040:CDS 20.0%
!!! TGAATTTTGCATTATTTGAA+GGG + chr4_3:9850350-9850369 Msa0629040:CDS 20.0%
!! TTATTTGAAGGGAAATATAA+TGG + chr4_3:9850361-9850380 Msa0629040:CDS 20.0%
! AAGTTAGTTGTGTTAAACTT+CGG - chr4_3:9850205-9850224 None:intergenic 25.0%
! AAGTTTAACACAACTAACTT+CGG + chr4_3:9850205-9850224 Msa0629040:CDS 25.0%
! AAGAAATGATGCTTATGAAA+AGG + chr4_3:9850405-9850424 Msa0629040:CDS 25.0%
TGTCTTCTTCTGAACAATTT+AGG - chr4_3:9850100-9850119 None:intergenic 30.0%
! TTAGCCACTTTAGATTCTAT+TGG + chr4_3:9850125-9850144 Msa0629040:CDS 30.0%
!!! TGGACTTTTTAGATTTGCTA+AGG + chr4_3:9850456-9850475 Msa0629040:CDS 30.0%
!!! GGACTTTTTAGATTTGCTAA+GGG + chr4_3:9850457-9850476 Msa0629040:CDS 30.0%
!! TACTTTTTGAATACCACTAC+AGG + chr4_3:9850499-9850518 Msa0629040:CDS 30.0%
CATGCCAATAGAATCTAAAG+TGG - chr4_3:9850132-9850151 None:intergenic 35.0%
ACCTCAATTGAGCTCAAAAT+TGG + chr4_3:9850264-9850283 Msa0629040:CDS 35.0%
! ACCAATTTTGAGCTCAATTG+AGG - chr4_3:9850268-9850287 None:intergenic 35.0%
CAATTGAGCTCAAAATTGGT+TGG + chr4_3:9850268-9850287 Msa0629040:CDS 35.0%
! TGGAAGCACAATTACTATCT+TGG + chr4_3:9850381-9850400 Msa0629040:CDS 35.0%
! GGAAGCACAATTACTATCTT+GGG + chr4_3:9850382-9850401 Msa0629040:CDS 35.0%
AGGTGGAACAATTTCTATCG+CGG - chr4_3:9850179-9850198 None:intergenic 40.0%
GTTGTGTTAAACTTCGGAAG+AGG - chr4_3:9850199-9850218 None:intergenic 40.0%
TAACACAACTAACTTCGGTG+AGG + chr4_3:9850210-9850229 Msa0629040:CDS 40.0%
TGAGCTCAAAATTGGTTGGA+AGG + chr4_3:9850272-9850291 Msa0629040:CDS 40.0%
GAGCTCAAAATTGGTTGGAA+GGG + chr4_3:9850273-9850292 Msa0629040:CDS 40.0%
AAGCTCAGTTTGTGCAGTAT+AGG - chr4_3:9850314-9850333 None:intergenic 40.0%
GTTAGAGAAATGCCTGTGAT+TGG + chr4_3:9850427-9850446 Msa0629040:CDS 40.0%
! TTGTGAGGGTGTAACAAGAG+AGG - chr4_3:9850033-9850052 None:intergenic 45.0%
TTGTGCTGTGACGATTGTGA+GGG - chr4_3:9850047-9850066 None:intergenic 45.0%
ATCTATTGAGCCCACGAAAC+CGG - chr4_3:9850077-9850096 None:intergenic 45.0%
TATTGGCATGACGTAGTCAG+CGG + chr4_3:9850142-9850161 Msa0629040:CDS 45.0%
TGGAACAATTTCTATCGCGG+TGG - chr4_3:9850176-9850195 None:intergenic 45.0%
GTGTTAAACTTCGGAAGAGG+TGG - chr4_3:9850196-9850215 None:intergenic 45.0%
ATAGACAACGCTTTGACCTC+AGG + chr4_3:9850241-9850260 Msa0629040:CDS 45.0%
AAATTGGTTGGAAGGGCTCA+AGG + chr4_3:9850280-9850299 Msa0629040:CDS 45.0%
AATTGGTTGGAAGGGCTCAA+GGG + chr4_3:9850281-9850300 Msa0629040:CDS 45.0%
AGAGAAATGCCTGTGATTGG+TGG + chr4_3:9850430-9850449 Msa0629040:CDS 45.0%
AGTTGTAGCATCTCCTGTAG+TGG - chr4_3:9850515-9850534 None:intergenic 45.0%
GGGTGTAACAAGAGAGGAGA+AGG - chr4_3:9850027-9850046 None:intergenic 50.0%
CTTGTGCTGTGACGATTGTG+AGG - chr4_3:9850048-9850067 None:intergenic 50.0%
GTCACAGCACAAGATGAGAC+CGG + chr4_3:9850055-9850074 Msa0629040:CDS 50.0%
! ACAAGATGAGACCGGTTTCG+TGG + chr4_3:9850063-9850082 Msa0629040:CDS 50.0%
! CAAGATGAGACCGGTTTCGT+GGG + chr4_3:9850064-9850083 Msa0629040:CDS 50.0%
TGAGCTCAATTGAGGTCCTG+AGG - chr4_3:9850260-9850279 None:intergenic 50.0%
ATGCCTGTGATTGGTGGAAG+TGG + chr4_3:9850436-9850455 Msa0629040:CDS 50.0%
AGTCCACTTCCACCAATCAC+AGG - chr4_3:9850442-9850461 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 9849992 9850567 9849992 ID=Msa0629040;Name=Msa0629040
chr4_3 mRNA 9849992 9850567 9849992 ID=Msa0629040-mRNA-1;Parent=Msa0629040;Name=Msa0629040-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|191
chr4_3 exon 9849992 9850567 9849992 ID=Msa0629040-mRNA-1:exon:2959;Parent=Msa0629040-mRNA-1
chr4_3 CDS 9849992 9850567 9849992 ID=Msa0629040-mRNA-1:cds;Parent=Msa0629040-mRNA-1
Gene Sequence

>Msa0629040

ATGGCTATAGGTGCTTTCAAAATCTTCTTCTTCCTTCTCCTCTCTTGTTACACCCTCACAATCGTCACAGCACAAGATGAGACCGGTTTCGTGGGCTCAATAGATCCTAAATTGTTCAGAAGAAGACAAAATGTTAGCCACTTTAGATTCTATTGGCATGACGTAGTCAGCGGAGACAACGCCACCGCGATAGAAATTGTTCCACCTCTTCCGAAGTTTAACACAACTAACTTCGGTGAGGTTAAAGTCATAGACAACGCTTTGACCTCAGGACCTCAATTGAGCTCAAAATTGGTTGGAAGGGCTCAAGGGATATATGCCTATACTGCACAAACTGAGCTTAATTTCCTTATGATAATGAATTTTGCATTATTTGAAGGGAAATATAATGGAAGCACAATTACTATCTTGGGAAGAAATGATGCTTATGAAAAGGTTAGAGAAATGCCTGTGATTGGTGGAAGTGGACTTTTTAGATTTGCTAAGGGATATGCTGAACTTACTACTTACTTTTTGAATACCACTACAGGAGATGCTACAACTGAGTACAATATTTATGTTTCTCATTATGTTTAA

Protein sequence

>Msa0629040

MAIGAFKIFFFLLLSCYTLTIVTAQDETGFVGSIDPKLFRRRQNVSHFRFYWHDVVSGDNATAIEIVPPLPKFNTTNFGEVKVIDNALTSGPQLSSKLVGRAQGIYAYTAQTELNFLMIMNFALFEGKYNGSTITILGRNDAYEKVREMPVIGGSGLFRFAKGYAELTTYFLNTTTGDATTEYNIYVSHYV*