AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0672780


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0672780 MtrunA17_Chr4g0013381 62.810 121 24 3 1 111 1 110 1.03e-33 114
Msa0672780 MtrunA17_Chr4g0013421 53.390 118 46 2 10 118 277 394 6.90e-18 78.2
Msa0672780 MtrunA17_Chr4g0013421 52.294 109 51 1 10 118 227 334 1.16e-17 77.4
Msa0672780 MtrunA17_Chr4g0013421 50.000 120 51 2 8 118 175 294 3.47e-16 73.6
Msa0672780 MtrunA17_Chr4g0013421 50.000 110 54 1 10 119 87 195 3.02e-15 70.9
Msa0672780 MtrunA17_Chr4g0013421 48.387 124 40 4 21 120 3 126 3.76e-14 67.4
Msa0672780 MtrunA17_Chr4g0013421 40.411 146 58 4 5 126 357 497 7.48e-12 60.8
Msa0672780 MtrunA17_Chr4g0013451 52.294 109 51 1 10 118 192 299 1.21e-17 77.4
Msa0672780 MtrunA17_Chr4g0013451 53.043 115 46 3 10 118 87 199 1.08e-16 74.7
Msa0672780 MtrunA17_Chr4g0013451 46.269 134 47 3 10 118 242 375 6.91e-14 66.6
Msa0672780 MtrunA17_Chr4g0013451 47.967 123 50 3 10 118 137 259 6.98e-14 66.6
Msa0672780 MtrunA17_Chr4g0013451 48.438 128 52 3 5 118 27 154 7.69e-14 66.6
Msa0672780 MtrunA17_Chr4g0013451 47.581 124 41 4 21 120 3 126 1.07e-13 66.2
Msa0672780 MtrunA17_Chr4g0013451 36.810 163 62 5 4 126 307 468 2.67e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 53 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGACTGGAGGTTTCTCAAC+TGG 0.297152 4_4:-18178954 Msa0672780:CDS
AACCGGAGGCTTGTCATAAT+TGG 0.298691 4_4:-18178397 Msa0672780:CDS
GTCGAGAAGCCACCAGTTTA+CGG 0.299440 4_4:+18179002 None:intergenic
TGCTCTCTTTGTCATCCCTC+AGG 0.337569 4_4:+18178368 None:intergenic
ACTACTGGTGGCTTGTAAAC+TGG 0.353333 4_4:-18178654 Msa0672780:CDS
TACACTGGTGGTTGGTAAAC+CGG 0.382977 4_4:-18178414 Msa0672780:CDS
CTCAACTGGAGGCTTGTAAA+CGG 0.405571 4_4:-18178895 Msa0672780:CDS
TCAACTGGTGGCTTATACAC+TGG 0.412257 4_4:-18178429 Msa0672780:CDS
TACACCGGTGGCTTGTAAAC+AGG 0.419007 4_4:-18178579 Msa0672780:CDS
TAGACTGGAGGCTTCTCAAC+TGG 0.423306 4_4:-18178909 Msa0672780:CDS
AGACTGGAGGTTTCTCAACT+GGG 0.425047 4_4:-18178953 Msa0672780:CDS
TGGTGAGGAGGTCCGTAAAC+TGG 0.427954 4_4:-18179014 Msa0672780:CDS
TAAACTGGTGGCTTCTCGAC+TGG 0.430061 4_4:-18178999 Msa0672780:CDS
TCGACTGGAGGCTTGTAAAC+TGG 0.436990 4_4:-18178984 Msa0672780:CDS
TAAACTGGTGGCTTGTAGAC+TGG 0.441265 4_4:-18178969 Msa0672780:CDS
TAAACGGGTGGCTTGTAGAC+TGG 0.441414 4_4:-18178879 Msa0672780:CDS
TAAACAGGTGGCTTGTAGAC+TGG 0.443434 4_4:-18178924 Msa0672780:CDS
TATACCGGAGGCTTGTAAAC+AGG 0.449409 4_4:-18178624 Msa0672780:CDS
TAAACAGGAGGCTTCTCAAC+TGG 0.459812 4_4:-18178609 Msa0672780:CDS
TCAACTGGTGGCTTGTACAC+CGG 0.460751 4_4:-18178594 Msa0672780:CDS
TATACAGGTGGCTTCACTAC+TGG 0.464722 4_4:-18178669 Msa0672780:intron
GCTCTCTTTGTCATCCCTCA+GGG 0.466378 4_4:+18178369 None:intergenic
TAGACTGGAGGCTTGTATAC+AGG 0.470954 4_4:-18178864 Msa0672780:intron
TCAACTGGAGGCTTGTAAAC+GGG 0.476504 4_4:-18178894 Msa0672780:CDS
GCCTCCTGTTTACAAGCCTC+CGG 0.504860 4_4:+18178620 None:intergenic
TGCCAATTATGACAAGCCTC+CGG 0.514734 4_4:+18178395 None:intergenic
TAAACTGGAGGCTTGTATAC+CGG 0.526226 4_4:-18178639 Msa0672780:CDS
GACTGGAGGTTTCTCAACTG+GGG 0.534766 4_4:-18178952 Msa0672780:CDS
GGGAACTATGGGTGGTGAGG+AGG 0.563963 4_4:-18179026 None:intergenic
ACTGGAGGTTTCTCAACTGG+GGG 0.581297 4_4:-18178951 Msa0672780:CDS
CGAGGGAACTATGGGTGGTG+AGG 0.583741 4_4:-18179029 None:intergenic
ACCTCCTGTTTACAAGCCAC+CGG 0.585895 4_4:+18178575 None:intergenic
ACAGGTGGCTTCACTACTGG+TGG 0.613745 4_4:-18178666 Msa0672780:intron
ACTGGTGGCTTCTCGACTGG+AGG 0.616423 4_4:-18178996 Msa0672780:CDS
ACTGGAGGCTTGTAAACTGG+TGG 0.629358 4_4:-18178981 Msa0672780:CDS
ACTGGTGGCTTATACACTGG+TGG 0.631560 4_4:-18178426 Msa0672780:CDS
TCATAATTGGCAAGACCCTG+AGG 0.637663 4_4:-18178384 Msa0672780:CDS
ACCGGTGGCTTGTAAACAGG+AGG 0.639427 4_4:-18178576 Msa0672780:intron
TGAGGAGGTCCGTAAACTGG+TGG 0.639666 4_4:-18179011 Msa0672780:CDS
ACAGGAGGCTTCTCAACTGG+TGG 0.640818 4_4:-18178606 Msa0672780:CDS
ACCGGAGGCTTGTAAACAGG+AGG 0.642069 4_4:-18178621 Msa0672780:CDS
CATAATTGGCAAGACCCTGA+GGG 0.642655 4_4:-18178383 Msa0672780:CDS
ACTGGTGGCTTGTACACCGG+TGG 0.645745 4_4:-18178591 Msa0672780:CDS
ACTGGAGGCTTCTCAACTGG+AGG 0.646341 4_4:-18178906 Msa0672780:CDS
ACTGGAGGCTTGTAAACGGG+TGG 0.650242 4_4:-18178891 Msa0672780:CDS
GTGGCTTATACACTGGTGGT+TGG 0.651466 4_4:-18178422 Msa0672780:CDS
ACTGGTGGCTTGTAGACTGG+AGG 0.655799 4_4:-18178966 Msa0672780:CDS
CTACCCGAGGGAACTATGGG+TGG 0.657162 4_4:-18179034 None:intergenic
ACTGGAGGCTTGTATACCGG+AGG 0.673497 4_4:-18178636 Msa0672780:CDS
ACGGGTGGCTTGTAGACTGG+AGG 0.678574 4_4:-18178876 Msa0672780:CDS
ACTGGTGGCTTGTAAACTGG+AGG 0.680586 4_4:-18178651 Msa0672780:CDS
ACAGGTGGCTTGTAGACTGG+AGG 0.680593 4_4:-18178921 Msa0672780:CDS
ACTGGTGGTTGGTAAACCGG+AGG 0.711189 4_4:-18178411 Msa0672780:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TAAACAGGAGGTTTGTAAAC+CGG - chr4_4:18178834-18178853 Msa0672780:intron 35.0%
TATACAGGTGGCTTAACTAC+TGG - chr4_4:18178549-18178568 Msa0672780:intron 40.0%
TAAACAGGAGGCTTGTAAAC+AGG - chr4_4:18178639-18178658 Msa0672780:CDS 40.0%
TAAACTGGAGGCTTGTATAC+CGG - chr4_4:18178759-18178778 Msa0672780:intron 40.0%
AAACAGGAGGTTTGTAAACC+GGG - chr4_4:18178835-18178854 Msa0672780:intron 40.0%
! TAAACTGGTGGCTTGTAGAC+TGG - chr4_4:18178429-18178448 Msa0672780:CDS 45.0%
TAGACTGGAGGTTTCTCAAC+TGG - chr4_4:18178444-18178463 Msa0672780:CDS 45.0%
AGACTGGAGGTTTCTCAACT+GGG - chr4_4:18178445-18178464 Msa0672780:CDS 45.0%
! TAAACAGGTGGCTTGTAGAC+TGG - chr4_4:18178474-18178493 Msa0672780:intron 45.0%
CTCAACTGGAGGCTTGTAAA+CGG - chr4_4:18178503-18178522 Msa0672780:intron 45.0%
TCAACTGGAGGCTTGTAAAC+GGG - chr4_4:18178504-18178523 Msa0672780:intron 45.0%
TAGACTGGAGGCTTGTATAC+AGG - chr4_4:18178534-18178553 Msa0672780:intron 45.0%
! ACTACTGGTGGCTTGTAAAC+TGG - chr4_4:18178564-18178583 Msa0672780:intron 45.0%
! TAAACTGGTGGCTTGTACAC+CGG - chr4_4:18178579-18178598 Msa0672780:CDS 45.0%
TAAACAGGAGGCTTCTCAAC+TGG - chr4_4:18178609-18178628 Msa0672780:CDS 45.0%
! TCAACTGGTGGCTTGTAAAC+AGG - chr4_4:18178624-18178643 Msa0672780:CDS 45.0%
TAAACAGGAGGCTTGTACAC+CGG - chr4_4:18178654-18178673 Msa0672780:CDS 45.0%
TAGACTGGAGGCTTGTATAC+AGG - chr4_4:18178534-18178553 Msa0672780:intron 45.0%
TATACAGGTGGCTTCACTAC+TGG - chr4_4:18178729-18178748 Msa0672780:intron 45.0%
! ACTACTGGTGGCTTGTAAAC+TGG - chr4_4:18178564-18178583 Msa0672780:intron 45.0%
TATACCGGAGGCTTGTAAAC+AGG - chr4_4:18178774-18178793 Msa0672780:intron 45.0%
TAAACAGGAGGCTTCTCAAC+TGG - chr4_4:18178609-18178628 Msa0672780:CDS 45.0%
AACAGGAGGTTTGTAAACCG+GGG - chr4_4:18178836-18178855 Msa0672780:intron 45.0%
! TACACAGGTGGCTTTTCTAC+CGG - chr4_4:18178879-18178898 Msa0672780:CDS 45.0%
TACACAGGTGGCTTATACAC+AGG - chr4_4:18178909-18178928 Msa0672780:CDS 45.0%
TCAACTGGTGGCTTATACAC+TGG - chr4_4:18178969-18178988 Msa0672780:CDS 45.0%
!! TACACTGGTGGTTGGTAAAC+CGG - chr4_4:18178984-18179003 Msa0672780:CDS 45.0%
AACCGGAGGCTTGTCATAAT+TGG - chr4_4:18179001-18179020 Msa0672780:CDS 45.0%
TGCCAATTATGACAAGCCTC+CGG + chr4_4:18179006-18179025 None:intergenic 45.0%
TCATAATTGGCAAGACCCTG+AGG - chr4_4:18179014-18179033 Msa0672780:CDS 45.0%
CATAATTGGCAAGACCCTGA+GGG - chr4_4:18179015-18179034 Msa0672780:CDS 45.0%
GTCGAGAAGCCACCAGTTTA+CGG + chr4_4:18178399-18178418 None:intergenic 50.0%
TAAACTGGTGGCTTCTCGAC+TGG - chr4_4:18178399-18178418 Msa0672780:CDS 50.0%
TCGACTGGAGGCTTGTAAAC+TGG - chr4_4:18178414-18178433 Msa0672780:CDS 50.0%
ACTGGAGGCTTGTAAACTGG+TGG - chr4_4:18178417-18178436 Msa0672780:CDS 50.0%
GACTGGAGGTTTCTCAACTG+GGG - chr4_4:18178446-18178465 Msa0672780:CDS 50.0%
ACTGGAGGTTTCTCAACTGG+GGG - chr4_4:18178447-18178466 Msa0672780:CDS 50.0%
TCAACTGGGGGCTTGTAAAC+AGG - chr4_4:18178459-18178478 Msa0672780:intron 50.0%
TAGACTGGAGGCTTCTCAAC+TGG - chr4_4:18178489-18178508 Msa0672780:intron 50.0%
! TAAACGGGTGGCTTGTAGAC+TGG - chr4_4:18178519-18178538 Msa0672780:intron 50.0%
ACTGGAGGCTTGTATACAGG+TGG - chr4_4:18178537-18178556 Msa0672780:intron 50.0%
ACAGGTGGCTTAACTACTGG+TGG - chr4_4:18178552-18178571 Msa0672780:intron 50.0%
! ACTGGTGGCTTGTAAACTGG+TGG - chr4_4:18178567-18178586 Msa0672780:intron 50.0%
TACACCGGAGGCTTGTAAAC+AGG - chr4_4:18178594-18178613 Msa0672780:CDS 50.0%
! ACTGGTGGCTTGTAAACAGG+AGG - chr4_4:18178627-18178646 Msa0672780:CDS 50.0%
ACAGGAGGCTTGTAAACAGG+AGG - chr4_4:18178642-18178661 Msa0672780:CDS 50.0%
TAGACTGGAGGCTTCTCAAC+TGG - chr4_4:18178489-18178508 Msa0672780:intron 50.0%
! TCAACTGGTGGCTTGTAGAC+TGG - chr4_4:18178699-18178718 Msa0672780:intron 50.0%
ACTGGAGGCTTGTATACAGG+TGG - chr4_4:18178537-18178556 Msa0672780:intron 50.0%
! ACTGGTGGCTTGTAAACTGG+AGG - chr4_4:18178567-18178586 Msa0672780:intron 50.0%
! TCAACTGGTGGCTTGTACAC+CGG - chr4_4:18178804-18178823 Msa0672780:intron 50.0%
! TACACCGGTGGCTTGTAAAC+AGG - chr4_4:18178819-18178838 Msa0672780:intron 50.0%
ACCTCCTGTTTACAAGCCAC+CGG + chr4_4:18178826-18178845 None:intergenic 50.0%
ACAGGAGGTTTGTAAACCGG+GGG - chr4_4:18178837-18178856 Msa0672780:intron 50.0%
! ACACAGGTGGCTTTTCTACC+GGG - chr4_4:18178880-18178899 Msa0672780:CDS 50.0%
ACAGGTGGCTTATACACAGG+TGG - chr4_4:18178912-18178931 Msa0672780:CDS 50.0%
TACACAGGTGGCTTCTCTAC+AGG - chr4_4:18178924-18178943 Msa0672780:CDS 50.0%
ACACAGGTGGCTTCTCTACA+GGG - chr4_4:18178925-18178944 Msa0672780:CDS 50.0%
TCTACAGGGGGTTTGTAGAC+AGG - chr4_4:18178939-18178958 Msa0672780:CDS 50.0%
CTACAGGGGGTTTGTAGACA+GGG - chr4_4:18178940-18178959 Msa0672780:CDS 50.0%
TACAGGGGGTTTGTAGACAG+GGG - chr4_4:18178941-18178960 Msa0672780:CDS 50.0%
TAGACAGGGGGTTTCTCAAC+TGG - chr4_4:18178954-18178973 Msa0672780:CDS 50.0%
ACTGGTGGCTTATACACTGG+TGG - chr4_4:18178972-18178991 Msa0672780:CDS 50.0%
GTGGCTTATACACTGGTGGT+TGG - chr4_4:18178976-18178995 Msa0672780:CDS 50.0%
TGGTGAGGAGGTCCGTAAAC+TGG - chr4_4:18178384-18178403 Msa0672780:CDS 55.0%
TGAGGAGGTCCGTAAACTGG+TGG - chr4_4:18178387-18178406 Msa0672780:CDS 55.0%
! ACTGGTGGCTTGTAGACTGG+AGG - chr4_4:18178432-18178451 Msa0672780:CDS 55.0%
ACTGGGGGCTTGTAAACAGG+TGG - chr4_4:18178462-18178481 Msa0672780:intron 55.0%
! ACAGGTGGCTTGTAGACTGG+AGG - chr4_4:18178477-18178496 Msa0672780:intron 55.0%
ACTGGAGGCTTCTCAACTGG+AGG - chr4_4:18178492-18178511 Msa0672780:intron 55.0%
ACTGGAGGCTTGTAAACGGG+TGG - chr4_4:18178507-18178526 Msa0672780:intron 55.0%
ACCGGAGGCTTGTAAACAGG+AGG - chr4_4:18178597-18178616 Msa0672780:CDS 55.0%
GCCTCCTGTTTACAAGCCTC+CGG + chr4_4:18178601-18178620 None:intergenic 55.0%
ACAGGAGGCTTCTCAACTGG+TGG - chr4_4:18178612-18178631 Msa0672780:CDS 55.0%
! TACACCGGTGGCTTGTAGAC+TGG - chr4_4:18178669-18178688 Msa0672780:intron 55.0%
ACTGGAGGCTTCTCAACTGG+TGG - chr4_4:18178492-18178511 Msa0672780:intron 55.0%
! ACTGGTGGCTTGTAGACTGG+AGG - chr4_4:18178432-18178451 Msa0672780:CDS 55.0%
ACAGGTGGCTTCACTACTGG+TGG - chr4_4:18178732-18178751 Msa0672780:intron 55.0%
ACTGGAGGCTTGTATACCGG+AGG - chr4_4:18178762-18178781 Msa0672780:intron 55.0%
ACCGGAGGCTTGTAAACAGG+AGG - chr4_4:18178597-18178616 Msa0672780:CDS 55.0%
GCCTCCTGTTTACAAGCCTC+CGG + chr4_4:18178601-18178620 None:intergenic 55.0%
ACAGGAGGCTTCTCAACTGG+TGG - chr4_4:18178612-18178631 Msa0672780:CDS 55.0%
! ACCGGTGGCTTGTAAACAGG+AGG - chr4_4:18178822-18178841 Msa0672780:intron 55.0%
TAAACCGGGGGTTTCACGAC+AGG - chr4_4:18178849-18178868 Msa0672780:intron 55.0%
AAACCGGGGGTTTCACGACA+GGG - chr4_4:18178850-18178869 Msa0672780:intron 55.0%
ACGACAGGGGGTTTGTACAC+AGG - chr4_4:18178864-18178883 Msa0672780:intron 55.0%
ACAGGGGGTTTGTACACAGG+TGG - chr4_4:18178867-18178886 Msa0672780:CDS 55.0%
! CACAGGTGGCTTTTCTACCG+GGG - chr4_4:18178881-18178900 Msa0672780:CDS 55.0%
! ACAGGTGGCTTTTCTACCGG+GGG - chr4_4:18178882-18178901 Msa0672780:CDS 55.0%
CACAGGTGGCTTCTCTACAG+GGG - chr4_4:18178926-18178945 Msa0672780:CDS 55.0%
ACAGGTGGCTTCTCTACAGG+GGG - chr4_4:18178927-18178946 Msa0672780:CDS 55.0%
ACAGGGGGTTTGTAGACAGG+GGG - chr4_4:18178942-18178961 Msa0672780:CDS 55.0%
ACAGGGGGTTTCTCAACTGG+TGG - chr4_4:18178957-18178976 Msa0672780:CDS 55.0%
!! ACTGGTGGTTGGTAAACCGG+AGG - chr4_4:18178987-18179006 Msa0672780:CDS 55.0%
ACTGGTGGCTTCTCGACTGG+AGG - chr4_4:18178402-18178421 Msa0672780:CDS 60.0%
! ACGGGTGGCTTGTAGACTGG+AGG - chr4_4:18178522-18178541 Msa0672780:intron 60.0%
! ACTGGTGGCTTGTACACCGG+AGG - chr4_4:18178582-18178601 Msa0672780:CDS 60.0%
ACAGGAGGCTTGTACACCGG+TGG - chr4_4:18178657-18178676 Msa0672780:CDS 60.0%
! ACCGGTGGCTTGTAGACTGG+AGG - chr4_4:18178672-18178691 Msa0672780:intron 60.0%
GCCTCCAGTCTACAAGCCAC+CGG + chr4_4:18178481-18178500 None:intergenic 60.0%
! ACTGGTGGCTTGTACACCGG+TGG - chr4_4:18178582-18178601 Msa0672780:CDS 60.0%
AACCGGGGGTTTCACGACAG+GGG - chr4_4:18178851-18178870 Msa0672780:intron 60.0%
TCTACCGGGGGCTTGTACAC+AGG - chr4_4:18178894-18178913 Msa0672780:CDS 60.0%
ACCGGGGGTTTCACGACAGG+GGG - chr4_4:18178852-18178871 Msa0672780:intron 65.0%
ACCCCCTGTCGTGAAACCCC+CGG + chr4_4:18178856-18178875 None:intergenic 65.0%
ACCGGGGGCTTGTACACAGG+TGG - chr4_4:18178897-18178916 Msa0672780:CDS 65.0%
GCCACCTGTGTACAAGCCCC+CGG + chr4_4:18178901-18178920 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 18178379 18179041 18178379 ID=Msa0672780;Name=Msa0672780
chr4_4 mRNA 18178379 18179041 18178379 ID=Msa0672780-mRNA-1;Parent=Msa0672780;Name=Msa0672780-mRNA-1;_AED=0.53;_eAED=0.89;_QI=0|0|0|1|0|0|3|0|126
chr4_4 exon 18178865 18179041 18178865 ID=Msa0672780-mRNA-1:exon:5327;Parent=Msa0672780-mRNA-1
chr4_4 exon 18178577 18178684 18178577 ID=Msa0672780-mRNA-1:exon:5326;Parent=Msa0672780-mRNA-1
chr4_4 exon 18178379 18178474 18178379 ID=Msa0672780-mRNA-1:exon:5325;Parent=Msa0672780-mRNA-1
chr4_4 CDS 18178865 18179041 18178865 ID=Msa0672780-mRNA-1:cds;Parent=Msa0672780-mRNA-1
chr4_4 CDS 18178577 18178684 18178577 ID=Msa0672780-mRNA-1:cds;Parent=Msa0672780-mRNA-1
chr4_4 CDS 18178379 18178474 18178379 ID=Msa0672780-mRNA-1:cds;Parent=Msa0672780-mRNA-1
Gene Sequence

>Msa0672780

ATGGGTGGTGAGGAGGTCCGTAAACTGGTGGCTTCTCGACTGGAGGCTTGTAAACTGGTGGCTTGTAGACTGGAGGTTTCTCAACTGGGGGCTTGTAAACAGGTGGCTTGTAGACTGGAGGCTTCTCAACTGGAGGCTTGTAAACGGGTGGCTTGTAGACTGGAGGCTTGTATACAGGTGGCTTCACTACTGGTGGCTTGTAAACTGGAGGCTTGTATACCGGAGGCTTGTAAACAGGAGGCTTCTCAACTGGTGGCTTGTACACCGGTGGCTTGTAAACAGGAGGGGGTTTGTAGACAGGGGGTTTCTCAACTGGTGGCTTATACACTGGTGGTTGGTAAACCGGAGGCTTGTCATAATTGGCAAGACCCTGAGGGATGA

Protein sequence

>Msa0672780

MGGEEVRKLVASRLEACKLVACRLEVSQLGACKQVACRLEASQLEACKRVACRLEACIQVASLLVACKLEACIPEACKQEASQLVACTPVACKQEGVCRQGVSQLVAYTLVVGKPEACHNWQDPEG*