AlfalfaGEDB | MS.gene011400

M. sativa cultivar XinJiangDaYe / MS.gene011400

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene011400.t1	XP_003608847.1	95.1	41	2	0	1	41	1	41	1.80E-12	82.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene011400.t1	G7JLF5	95.1	41	2	0	1	41	1	41	1.3e-12	82.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene011400	MS.gene64327	PPI
MS.gene011400	MS.gene28338	PPI
MS.gene011400	MS.gene87673	PPI
MS.gene011400	MS.gene53659	PPI
MS.gene64324	MS.gene011400	PPI
MS.gene011400	MS.gene061768	PPI
MS.gene011400	MS.gene89216	PPI
MS.gene011400	MS.gene64308	PPI
MS.gene011400	MS.gene009021	PPI
MS.gene011400	MS.gene47195	PPI
MS.gene011400	MS.gene017446	PPI
MS.gene011400	MS.gene96914	PPI
MS.gene011400	MS.gene066862	PPI
MS.gene011400	MS.gene96913	PPI
MS.gene80011	MS.gene011400	PPI
MS.gene070168	MS.gene011400	PPI
MS.gene003880	MS.gene011400	PPI
MS.gene011400	MS.gene07422	PPI
MS.gene011400	MS.gene062239	PPI
MS.gene011400	MS.gene015658	PPI
MS.gene011400	MS.gene64313	PPI
MS.gene011400	MS.gene80012	PPI
MS.gene011400	MS.gene061261	PPI
MS.gene011400	MS.gene036930	PPI
MS.gene004899	MS.gene011400	PPI
MS.gene011400	MS.gene21932	PPI
MS.gene011400	MS.gene052672	PPI
MS.gene011400	MS.gene026618	PPI
MS.gene011400	MS.gene53648	PPI
MS.gene011400	MS.gene015470	PPI
MS.gene011400	MS.gene008831	PPI
MS.gene011400	MS.gene55545	PPI
MS.gene011400	MS.gene056461	PPI
MS.gene070167	MS.gene011400	PPI
MS.gene011400	MS.gene98972	PPI
MS.gene011400	MS.gene29459	PPI
MS.gene011400	MS.gene71207	PPI
MS.gene011400	MS.gene92775	PPI
MS.gene011400	MS.gene21155	PPI
MS.gene011400	MS.gene53662	PPI
MS.gene011400	MS.gene63281	PPI
MS.gene011400	MS.gene020515	PPI
MS.gene68345	MS.gene011400	PPI
MS.gene011400	MS.gene013748	PPI
MS.gene011400	MS.gene008225	PPI
MS.gene011400	MS.gene21696	PPI
MS.gene011400	MS.gene64316	PPI
MS.gene011400	MS.gene05284	PPI
MS.gene011400	MS.gene22553	PPI
MS.gene038456	MS.gene011400	PPI
MS.gene011400	MS.gene074045	PPI
MS.gene011400	MS.gene03228	PPI
MS.gene011400	MS.gene000738	PPI
MS.gene011400	MS.gene46237	PPI
MS.gene011400	MS.gene056383	PPI
MS.gene011400	MS.gene64317	PPI
MS.gene011400	MS.gene025777	PPI
MS.gene011400	MS.gene63000	PPI
MS.gene011400	MS.gene93087	PPI
MS.gene011400	MS.gene64310	PPI
MS.gene011400	MS.gene046988	PPI
MS.gene58860	MS.gene011400	PPI
MS.gene011400	MS.gene057293	PPI
MS.gene011400	MS.gene09934	PPI
MS.gene011400	MS.gene58906	PPI
MS.gene011400	MS.gene79028	PPI
MS.gene011400	MS.gene64311	PPI
MS.gene011400	MS.gene053319	PPI
MS.gene011400	MS.gene000724	PPI
MS.gene011400	MS.gene65954	PPI
MS.gene62258	MS.gene011400	PPI
MS.gene011400	MS.gene001246	PPI
MS.gene011400	MS.gene041795	PPI
MS.gene011400	MS.gene85596	PPI
MS.gene011400	MS.gene96922	PPI
MS.gene011400	MS.gene25186	PPI
MS.gene011400	MS.gene80013	PPI
MS.gene011400	MS.gene025797	PPI
MS.gene011400	MS.gene21931	PPI
MS.gene011400	MS.gene27516	PPI
MS.gene011400	MS.gene64314	PPI
MS.gene011400	MS.gene040127	PPI
MS.gene011400	MS.gene09960	PPI
MS.gene011400	MS.gene94551	PPI
MS.gene83068	MS.gene011400	PPI
MS.gene011400	MS.gene53650	PPI
MS.gene011400	MS.gene066863	PPI
MS.gene011400	MS.gene02545	PPI
MS.gene011400	MS.gene04915	PPI
MS.gene62689	MS.gene011400	PPI
MS.gene97948	MS.gene011400	PPI
MS.gene54637	MS.gene011400	PPI
MS.gene011400	MS.gene61009	PPI
MS.gene67281	MS.gene011400	PPI
MS.gene04917	MS.gene011400	PPI
MS.gene011400	MS.gene93763	PPI
MS.gene46886	MS.gene011400	PPI
MS.gene026530	MS.gene011400	PPI
MS.gene011400	MS.gene64307	PPI
MS.gene011400	MS.gene026940	PPI
MS.gene011400	MS.gene024717	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene011400.t1	MTR_4g103630	95.714	140	6	0	1	140	1	140	1.89e-91	261
MS.gene011400.t1	MTR_4g103655	95.000	140	7	0	1	140	1	140	1.08e-90	259
MS.gene011400.t1	MTR_4g103650	96.324	136	5	0	5	140	1	136	1.96e-88	253
MS.gene011400.t1	MTR_3g089070	73.571	140	37	0	1	140	1	140	9.95e-69	204
MS.gene011400.t1	MTR_3g089090	74.453	137	35	0	4	140	3	139	3.18e-68	202
MS.gene011400.t1	MTR_6g023460	59.701	134	54	0	6	139	8	141	1.10e-54	168
MS.gene011400.t1	MTR_8g078270	54.135	133	56	2	10	139	57	187	7.90e-38	127
MS.gene011400.t1	MTR_3g067610	42.537	134	74	1	6	139	5	135	1.73e-30	107
MS.gene011400.t1	MTR_8g107110	39.259	135	82	0	6	140	24	158	1.35e-27	100
MS.gene011400.t1	MTR_8g105230	43.182	132	74	1	6	136	36	167	1.92e-27	100
MS.gene011400.t1	MTR_5g079340	38.806	134	82	0	6	139	4	137	6.79e-27	97.8
MS.gene011400.t1	MTR_4g067270	39.130	138	81	2	6	140	5	142	1.96e-25	94.7
MS.gene011400.t1	MTR_1g032070	40.741	135	79	1	6	139	46	180	1.68e-24	93.6
MS.gene011400.t1	MTR_7g075040	38.000	150	83	3	1	140	42	191	1.91e-23	90.9
MS.gene011400.t1	MTR_1g046950	34.752	141	81	2	6	139	4	140	2.28e-23	89.0
MS.gene011400.t1	MTR_1g047100	34.559	136	87	1	6	139	4	139	6.68e-23	87.8
MS.gene011400.t1	MTR_6g079570	37.037	135	84	1	6	139	26	160	3.61e-22	87.0
MS.gene011400.t1	MTR_3g083470	38.519	135	72	3	6	139	93	217	4.34e-21	85.5
MS.gene011400.t1	MTR_7g087610	35.000	140	81	3	6	139	12	147	7.52e-21	82.8
MS.gene011400.t1	MTR_6g025320	35.714	140	80	3	6	139	12	147	8.55e-21	82.8
MS.gene011400.t1	MTR_5g088320	35.714	140	80	3	6	139	12	147	9.32e-21	82.4
MS.gene011400.t1	MTR_4g086260	35.821	134	85	1	6	139	57	189	1.09e-20	83.6
MS.gene011400.t1	MTR_1g076650	34.286	140	83	2	5	139	11	146	7.79e-20	80.1
MS.gene011400.t1	MTR_8g036075	35.417	144	84	3	6	140	49	192	3.62e-19	79.7
MS.gene011400.t1	MTR_7g089760	34.783	138	87	2	6	140	5	142	4.91e-19	78.2
MS.gene011400.t1	MTR_7g090450	33.793	145	82	4	6	140	73	213	6.10e-19	79.3
MS.gene011400.t1	MTR_1g041285	33.824	136	89	1	5	139	11	146	1.11e-18	77.4
MS.gene011400.t1	MTR_8g066630	32.847	137	82	2	6	135	17	150	3.99e-18	76.3
MS.gene011400.t1	MTR_2g098890	37.143	140	83	2	6	140	64	203	7.10e-18	76.6
MS.gene011400.t1	MTR_1g071150	32.593	135	90	1	6	139	12	146	7.36e-18	75.1
MS.gene011400.t1	MTR_5g017510	33.333	135	89	1	6	139	48	182	7.72e-18	75.9
MS.gene011400.t1	MTR_5g017560	33.333	135	89	1	6	139	48	182	7.72e-18	75.9
MS.gene011400.t1	MTR_7g074240	32.667	150	88	2	3	139	21	170	4.91e-17	73.6
MS.gene011400.t1	MTR_4g115170	33.766	154	83	3	6	140	64	217	3.33e-16	72.4
MS.gene011400.t1	MTR_5g017550	32.353	136	88	2	6	139	48	181	4.28e-16	71.2
MS.gene011400.t1	MTR_5g027000	32.031	128	86	1	14	140	30	157	3.66e-15	68.6
MS.gene011400.t1	MTR_5g009830	36.220	127	76	2	15	139	380	503	4.89e-15	70.9
MS.gene011400.t1	MTR_5g008705	31.538	130	82	2	14	139	2	128	7.96e-15	66.6
MS.gene011400.t1	MTR_4g112460	29.927	137	93	1	6	139	11	147	1.08e-14	67.4
MS.gene011400.t1	MTR_3g088655	32.667	150	88	4	3	139	5	154	3.98e-14	65.5
MS.gene011400.t1	MTR_8g099095	33.858	127	79	2	15	139	380	503	6.59e-14	67.8
MS.gene011400.t1	MTR_5g092810	32.576	132	84	2	10	139	401	529	9.41e-14	67.4
MS.gene011400.t1	MTR_6g007613	31.111	135	92	1	6	139	29	163	1.30e-13	64.7
MS.gene011400.t1	MTR_1g026190	28.148	135	92	2	7	139	388	519	2.31e-13	66.2
MS.gene011400.t1	MTR_5g008695	33.043	115	66	2	31	138	2	112	3.54e-13	62.0
MS.gene011400.t1	MTR_0168s0120	33.043	115	67	3	31	139	1	111	3.88e-13	62.0
MS.gene011400.t1	MTR_6g025320	33.043	115	67	3	31	139	1	111	3.88e-13	62.0
MS.gene011400.t1	MTR_3g051770	30.882	136	88	2	10	139	392	527	4.13e-13	65.5
MS.gene011400.t1	MTR_4g132070	32.283	127	81	2	15	139	405	528	1.38e-12	63.9
MS.gene011400.t1	MTR_1g030440	30.345	145	84	3	6	139	22	160	1.42e-12	61.6
MS.gene011400.t1	MTR_7g074020	30.370	135	93	1	6	139	30	164	1.44e-12	61.6
MS.gene011400.t1	MTR_8g069915	26.277	137	98	1	6	139	12	148	4.41e-12	60.5
MS.gene011400.t1	MTR_5g089320	28.467	137	93	2	5	139	372	505	5.97e-12	62.0
MS.gene011400.t1	MTR_0028s0170	29.921	127	84	2	15	139	392	515	9.52e-12	61.6
MS.gene011400.t1	MTR_4g066660	31.387	137	87	3	7	140	378	510	1.31e-11	61.2
MS.gene011400.t1	MTR_7g115040	31.852	135	91	1	6	139	12	146	1.31e-11	58.9
MS.gene011400.t1	MTR_1g052530	31.746	126	86	0	15	140	366	491	2.33e-11	60.5
MS.gene011400.t1	MTR_7g068710	29.078	141	97	1	3	140	355	495	8.42e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene011400.t1	AT1G76650	47.794	136	71	0	4	139	41	176	5.23e-37	125
MS.gene011400.t1	AT1G76650	47.794	136	71	0	4	139	41	176	5.23e-37	125
MS.gene011400.t1	AT5G42380	47.794	136	69	1	6	139	49	184	4.75e-34	117
MS.gene011400.t1	AT1G76650	46.324	136	63	1	4	139	41	166	3.93e-33	114
MS.gene011400.t1	AT1G76640	44.604	139	74	1	4	139	20	158	1.73e-32	112
MS.gene011400.t1	AT3G50770	44.755	143	73	2	3	139	61	203	9.09e-32	112
MS.gene011400.t1	AT1G24620	45.185	135	73	1	6	139	37	171	7.72e-31	109
MS.gene011400.t1	AT3G01830	43.885	139	71	4	6	140	11	146	2.06e-28	102
MS.gene011400.t1	AT1G73630	36.496	137	84	1	3	139	17	150	5.37e-26	96.3
MS.gene011400.t1	AT1G18210	38.129	139	83	1	1	139	18	153	9.46e-26	95.9
MS.gene011400.t1	AT1G18210	38.129	139	83	1	1	139	18	153	9.46e-26	95.9
MS.gene011400.t1	AT5G37770	35.971	139	84	1	6	139	17	155	1.02e-25	95.5
MS.gene011400.t1	AT5G37770	35.971	139	84	1	6	139	17	155	1.02e-25	95.5
MS.gene011400.t1	AT1G66400	33.577	137	88	1	6	139	15	151	8.32e-24	90.5
MS.gene011400.t1	AT4G03290	39.007	141	80	2	6	140	5	145	5.21e-23	88.6
MS.gene011400.t1	AT1G05990	38.129	139	82	2	6	140	5	143	8.86e-22	85.1
MS.gene011400.t1	AT3G59440	35.714	140	88	1	3	140	48	187	1.83e-21	85.5
MS.gene011400.t1	AT4G12860	37.956	137	82	2	6	139	5	141	2.20e-21	84.3
MS.gene011400.t1	AT5G37780	36.429	140	79	3	6	139	12	147	3.78e-21	83.6
MS.gene011400.t1	AT1G66410	36.429	140	79	3	6	139	12	147	3.78e-21	83.6
MS.gene011400.t1	AT5G21274	36.429	140	79	3	6	139	12	147	3.91e-21	83.6
MS.gene011400.t1	AT3G56800	35.714	140	80	3	6	139	12	147	6.35e-21	83.2
MS.gene011400.t1	AT2G27030	35.714	140	80	3	6	139	12	147	6.35e-21	83.2
MS.gene011400.t1	AT2G41110	35.714	140	80	3	6	139	12	147	6.35e-21	83.2
MS.gene011400.t1	AT3G43810	35.714	140	80	3	6	139	12	147	8.96e-21	82.8
MS.gene011400.t1	AT2G43290	34.722	144	84	3	6	140	65	207	1.40e-20	84.0
MS.gene011400.t1	AT2G27030	35.714	140	80	3	6	139	12	147	1.46e-20	82.8
MS.gene011400.t1	AT3G22930	34.307	137	88	2	5	139	34	170	2.53e-20	82.0
MS.gene011400.t1	AT5G17470	35.294	136	81	2	7	139	7	138	3.13e-20	81.3
MS.gene011400.t1	AT3G43810	35.714	140	80	3	6	139	12	147	3.57e-20	82.8
MS.gene011400.t1	AT2G15680	37.778	135	81	2	6	139	50	182	4.12e-20	82.0
MS.gene011400.t1	AT3G07490	36.957	138	84	2	6	140	5	142	6.21e-20	80.5
MS.gene011400.t1	AT4G14640	34.532	139	82	2	6	139	13	147	1.29e-18	77.0
MS.gene011400.t1	AT2G36180	34.328	134	84	2	10	139	4	137	1.60e-18	76.6
MS.gene011400.t1	AT3G10190	32.143	140	93	2	3	140	67	206	2.47e-18	77.8
MS.gene011400.t1	AT2G41110	32.895	152	80	4	6	139	12	159	3.82e-18	76.3
MS.gene011400.t1	AT2G41100	28.947	152	90	1	6	139	66	217	1.86e-17	76.6
MS.gene011400.t1	AT2G41100	28.947	152	90	1	6	139	66	217	1.86e-17	76.6
MS.gene011400.t1	AT2G41100	28.947	152	90	1	6	139	66	217	1.86e-17	76.6
MS.gene011400.t1	AT2G41100	28.947	152	90	1	6	139	101	252	2.95e-17	76.6
MS.gene011400.t1	AT2G41100	27.152	151	93	1	6	139	12	162	3.46e-14	68.2
MS.gene011400.t1	AT2G41100	28.947	152	90	1	6	139	101	252	2.95e-17	76.6
MS.gene011400.t1	AT2G41100	27.152	151	93	1	6	139	12	162	3.46e-14	68.2
MS.gene011400.t1	AT5G37780	32.903	155	79	4	6	139	12	162	4.47e-17	73.6
MS.gene011400.t1	AT3G50360	31.852	135	91	1	6	139	27	161	1.21e-15	69.7
MS.gene011400.t1	AT4G23650	34.884	129	79	2	14	140	391	516	2.07e-15	72.0
MS.gene011400.t1	AT5G37780	30.723	166	79	4	6	139	12	173	2.43e-15	69.3
MS.gene011400.t1	AT3G51920	31.852	135	91	1	6	139	12	146	4.32e-15	68.2
MS.gene011400.t1	AT4G20780	30.769	156	87	3	5	139	28	183	6.47e-15	68.6
MS.gene011400.t1	AT3G03400	33.088	136	82	4	3	132	2	134	8.59e-15	67.0
MS.gene011400.t1	AT5G37780	33.858	127	72	3	20	139	13	134	8.86e-15	67.0
MS.gene011400.t1	AT1G66410	34.127	126	73	3	20	139	36	157	9.86e-15	67.4
MS.gene011400.t1	AT1G18530	29.197	137	94	1	6	139	7	143	1.22e-14	67.0
MS.gene011400.t1	AT4G37010	28.676	136	96	1	6	140	31	166	2.21e-14	66.6
MS.gene011400.t1	AT4G37010	28.676	136	96	1	6	140	27	162	2.75e-14	66.2
MS.gene011400.t1	AT4G04695	31.111	135	88	2	7	139	337	468	2.96e-14	68.9
MS.gene011400.t1	AT4G04700	31.852	135	87	2	7	139	337	468	6.61e-14	67.8
MS.gene011400.t1	AT2G41090	31.111	135	90	2	6	139	12	144	1.10e-13	65.1
MS.gene011400.t1	AT1G66410	33.913	115	66	3	31	139	1	111	1.75e-13	62.8
MS.gene011400.t1	AT1G66410	33.913	115	66	3	31	139	1	111	1.75e-13	62.8
MS.gene011400.t1	AT4G14640	32.800	125	75	2	20	139	9	129	2.52e-13	63.2
MS.gene011400.t1	AT1G62820	35.714	126	72	4	14	135	21	141	3.67e-13	62.8
MS.gene011400.t1	AT3G43810	33.043	115	67	3	31	139	1	111	4.06e-13	62.0
MS.gene011400.t1	AT3G43810	33.043	115	67	3	31	139	1	111	4.06e-13	62.0
MS.gene011400.t1	AT1G12310	35.714	126	72	4	14	135	21	141	5.27e-13	62.4
MS.gene011400.t1	AT3G51850	30.952	126	87	0	15	140	368	493	6.53e-13	65.1
MS.gene011400.t1	AT2G41410	31.884	138	90	3	6	139	70	207	6.53e-13	63.5
MS.gene011400.t1	AT1G32250	29.286	140	86	2	6	135	16	152	1.12e-12	62.0
MS.gene011400.t1	AT5G44460	29.530	149	92	3	5	140	27	175	1.14e-12	62.4
MS.gene011400.t1	AT1G76040	33.071	127	80	2	15	139	187	310	1.25e-12	63.9
MS.gene011400.t1	AT1G76040	33.071	127	80	2	15	139	187	310	1.25e-12	63.9
MS.gene011400.t1	AT2G31500	34.014	147	85	5	3	140	365	508	1.47e-12	63.9
MS.gene011400.t1	AT4G04720	31.061	132	86	2	10	139	388	516	1.59e-12	63.9
MS.gene011400.t1	AT4G04720	31.061	132	86	2	10	139	388	516	1.59e-12	63.9
MS.gene011400.t1	AT1G76040	33.071	127	80	2	15	139	425	548	1.73e-12	63.9
MS.gene011400.t1	AT1G50700	30.303	132	87	2	10	139	382	510	1.78e-12	63.5
MS.gene011400.t1	AT5G19360	32.283	127	81	2	15	139	382	505	2.27e-12	63.5
MS.gene011400.t1	AT3G01830	41.509	106	55	4	6	107	11	113	2.32e-12	60.5
MS.gene011400.t1	AT3G25600	27.737	137	96	1	6	139	12	148	2.35e-12	61.2
MS.gene011400.t1	AT1G74740	29.545	132	91	2	10	140	368	498	3.39e-12	62.8
MS.gene011400.t1	AT3G20410	31.061	132	86	2	10	139	400	528	4.03e-12	62.8
MS.gene011400.t1	AT3G20410	31.061	132	86	2	10	139	400	528	4.03e-12	62.8
MS.gene011400.t1	AT3G03000	30.303	132	90	1	6	135	20	151	4.22e-12	60.5
MS.gene011400.t1	AT4G21940	32.283	127	81	2	15	139	415	538	4.29e-12	62.8
MS.gene011400.t1	AT4G21940	32.283	127	81	2	15	139	415	538	4.34e-12	62.8
MS.gene011400.t1	AT5G12180	31.496	127	82	2	15	139	387	510	1.49e-11	61.2
MS.gene011400.t1	AT5G12480	31.496	127	86	1	15	140	279	405	3.74e-11	59.7
MS.gene011400.t1	AT2G41100	29.630	108	58	1	50	139	20	127	6.82e-11	57.8
MS.gene011400.t1	AT1G61950	30.075	133	88	2	10	140	408	537	7.11e-11	58.9

Find 35 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGGATTTAATAACGCTTA+TGG	0.268235	8.3:+18725098	MS.gene011400:CDS
AACATCCTCTTCAAACTCTT+TGG	0.271094	8.3:-18725204	None:intergenic
CATCACTTTACATTCTTCAA+TGG	0.327118	8.3:-18725250	None:intergenic
GATGATTGGAAGGTTTGATT+TGG	0.354750	8.3:+18725269	MS.gene011400:CDS
TTAATAACGCTTATGGAATC+CGG	0.394530	8.3:+18725105	MS.gene011400:CDS
AGATTTCACCGAACGAGTTA+AGG	0.395728	8.3:+18724980	MS.gene011400:CDS
AAGAAAAGTTGAAGGATTTG+AGG	0.418092	8.3:+18725136	MS.gene011400:CDS
GGAAATTGCTATTGAGGCAT+TGG	0.422915	8.3:+18725047	MS.gene011400:CDS
TGGTGATGGGCTATTGAGTT+TGG	0.423743	8.3:+18725077	MS.gene011400:CDS
AGAAAAGTTGAAGGATTTGA+GGG	0.426919	8.3:+18725137	MS.gene011400:CDS
AGGAGTCGAATGACGAAGAT+TGG	0.473202	8.3:+18725000	MS.gene011400:CDS
ATTCGACTCCTTAACTCGTT+CGG	0.474759	8.3:-18724988	None:intergenic
GAAGAATGTAAAGTGATGAT+TGG	0.486801	8.3:+18725255	MS.gene011400:CDS
TAATAACGCTTATGGAATCC+GGG	0.494077	8.3:+18725106	MS.gene011400:CDS
AGAAGTGGAAATTGCTATTG+AGG	0.499700	8.3:+18725041	MS.gene011400:CDS
AATGTAAAGTGATGATTGGA+AGG	0.500397	8.3:+18725259	MS.gene011400:CDS
AGAGTTTCAATTGAAAGAAG+TGG	0.502556	8.3:+18725026	MS.gene011400:CDS
TGAAGAATTCAGAATCATGA+TGG	0.506151	8.3:+18725314	MS.gene011400:CDS
AGGTTTGATTTGGATGGTGA+TGG	0.507936	8.3:+18725279	MS.gene011400:CDS
ATTGGAAGGTTTGATTTGGA+TGG	0.510943	8.3:+18725273	MS.gene011400:CDS
TGATGGGCTATTGAGTTTGG+AGG	0.520299	8.3:+18725080	MS.gene011400:CDS
TTTGAAATGTATGATAATGA+AGG	0.534288	8.3:+18725165	MS.gene011400:CDS
ATGTATGATAATGAAGGATG+TGG	0.537649	8.3:+18725171	MS.gene011400:CDS
GCATTGGATTCTGATGGTGA+TGG	0.540839	8.3:+18725063	MS.gene011400:CDS
TACTTTGATGAAGACGGTGA+TGG	0.542295	8.3:+18724955	MS.gene011400:CDS
GGGAGAAGAAGAAAAGTTGA+AGG	0.545380	8.3:+18725128	MS.gene011400:CDS
CTAAGTTACTTTGATGAAGA+CGG	0.574980	8.3:+18724949	MS.gene011400:CDS
AGTCGAATGACGAAGATTGG+CGG	0.582253	8.3:+18725003	MS.gene011400:CDS
CATTGGATTCTGATGGTGAT+GGG	0.600002	8.3:+18725064	MS.gene011400:CDS
TAACACCAAAGAGTTTGAAG+AGG	0.608054	8.3:+18725199	MS.gene011400:CDS
AATTAACATGAGGAGTAACA+TGG	0.611175	8.3:+18724912	None:intergenic
ATTGAGGCATTGGATTCTGA+TGG	0.616237	8.3:+18725057	MS.gene011400:CDS
TGTATGATAATGAAGGATGT+GGG	0.638642	8.3:+18725172	MS.gene011400:CDS
AATAACGCTTATGGAATCCG+GGG	0.638897	8.3:+18725107	MS.gene011400:CDS
ATAACGCTTATGGAATCCGG+GGG	0.690847	8.3:+18725108	MS.gene011400:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGAAATGTATGATAATGA+AGG	+	chr8.3:18725165-18725184	MS.gene011400:CDS	20.0%
	AAGAAAAGTTGAAGGATTTG+AGG	+	chr8.3:18725136-18725155	MS.gene011400:CDS	30.0%
	AAGAGGATGTTGAAAAAGTT+GGG	+	chr8.3:18725216-18725235	MS.gene011400:CDS	30.0%
	AATGTAAAGTGATGATTGGA+AGG	+	chr8.3:18725259-18725278	MS.gene011400:CDS	30.0%
	AGAAAAGTTGAAGGATTTGA+GGG	+	chr8.3:18725137-18725156	MS.gene011400:CDS	30.0%
	AGAGTTTCAATTGAAAGAAG+TGG	+	chr8.3:18725026-18725045	MS.gene011400:CDS	30.0%
	CATCACTTTACATTCTTCAA+TGG	-	chr8.3:18725253-18725272	None:intergenic	30.0%
	GAAGAATGTAAAGTGATGAT+TGG	+	chr8.3:18725255-18725274	MS.gene011400:CDS	30.0%
	TGAAGAATTCAGAATCATGA+TGG	+	chr8.3:18725314-18725333	MS.gene011400:CDS	30.0%
	TTAATAACGCTTATGGAATC+CGG	+	chr8.3:18725105-18725124	MS.gene011400:CDS	30.0%
!	ATGTATGATAATGAAGGATG+TGG	+	chr8.3:18725171-18725190	MS.gene011400:CDS	30.0%
!	CTAAGTTACTTTGATGAAGA+CGG	+	chr8.3:18724949-18724968	MS.gene011400:CDS	30.0%
!	TGTATGATAATGAAGGATGT+GGG	+	chr8.3:18725172-18725191	MS.gene011400:CDS	30.0%
	AACATCCTCTTCAAACTCTT+TGG	-	chr8.3:18725207-18725226	None:intergenic	35.0%
	AGAGGATGTTGAAAAAGTTG+GGG	+	chr8.3:18725217-18725236	MS.gene011400:CDS	35.0%
	GAAGAGGATGTTGAAAAAGT+TGG	+	chr8.3:18725215-18725234	MS.gene011400:CDS	35.0%
	GATGATTGGAAGGTTTGATT+TGG	+	chr8.3:18725269-18725288	MS.gene011400:CDS	35.0%
	GGAGGATTTAATAACGCTTA+TGG	+	chr8.3:18725098-18725117	MS.gene011400:CDS	35.0%
	TAACACCAAAGAGTTTGAAG+AGG	+	chr8.3:18725199-18725218	MS.gene011400:CDS	35.0%
	TAATAACGCTTATGGAATCC+GGG	+	chr8.3:18725106-18725125	MS.gene011400:CDS	35.0%
!	AGAAGTGGAAATTGCTATTG+AGG	+	chr8.3:18725041-18725060	MS.gene011400:CDS	35.0%
!!	ATTGGAAGGTTTGATTTGGA+TGG	+	chr8.3:18725273-18725292	MS.gene011400:CDS	35.0%
	AATAACGCTTATGGAATCCG+GGG	+	chr8.3:18725107-18725126	MS.gene011400:CDS	40.0%
	AGATTTCACCGAACGAGTTA+AGG	+	chr8.3:18724980-18724999	MS.gene011400:CDS	40.0%
	ATTCGACTCCTTAACTCGTT+CGG	-	chr8.3:18724991-18725010	None:intergenic	40.0%
	GAGGATGTTGAAAAAGTTGG+GGG	+	chr8.3:18725218-18725237	MS.gene011400:CDS	40.0%
	GGGAGAAGAAGAAAAGTTGA+AGG	+	chr8.3:18725128-18725147	MS.gene011400:CDS	40.0%
	TACTTTGATGAAGACGGTGA+TGG	+	chr8.3:18724955-18724974	MS.gene011400:CDS	40.0%
!	CATTGGATTCTGATGGTGAT+GGG	+	chr8.3:18725064-18725083	MS.gene011400:CDS	40.0%
!	GGAAATTGCTATTGAGGCAT+TGG	+	chr8.3:18725047-18725066	MS.gene011400:CDS	40.0%
!!	AGGTTTGATTTGGATGGTGA+TGG	+	chr8.3:18725279-18725298	MS.gene011400:CDS	40.0%
!!	ATTGAGGCATTGGATTCTGA+TGG	+	chr8.3:18725057-18725076	MS.gene011400:CDS	40.0%
	AGGAGTCGAATGACGAAGAT+TGG	+	chr8.3:18725000-18725019	MS.gene011400:CDS	45.0%
	AGTCGAATGACGAAGATTGG+CGG	+	chr8.3:18725003-18725022	MS.gene011400:CDS	45.0%
	ATAACGCTTATGGAATCCGG+GGG	+	chr8.3:18725108-18725127	MS.gene011400:CDS	45.0%
!	CAACTTTTCTTCTTCTCCCC+CGG	-	chr8.3:18725127-18725146	None:intergenic	45.0%
!	GCATTGGATTCTGATGGTGA+TGG	+	chr8.3:18725063-18725082	MS.gene011400:CDS	45.0%
!!	TGATGGGCTATTGAGTTTGG+AGG	+	chr8.3:18725080-18725099	MS.gene011400:CDS	45.0%
!!	TGGTGATGGGCTATTGAGTT+TGG	+	chr8.3:18725077-18725096	MS.gene011400:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	18724919	18725341	18724919	ID=MS.gene011400
chr8.3	mRNA	18724919	18725341	18724919	ID=MS.gene011400.t1;Parent=MS.gene011400
chr8.3	exon	18724919	18725341	18724919	ID=MS.gene011400.t1.exon1;Parent=MS.gene011400.t1
chr8.3	CDS	18724919	18725341	18724919	ID=cds.MS.gene011400.t1;Parent=MS.gene011400.t1

Gene Sequence

>MS.gene011400

ATGAGGAGTAACATGGAATTTGAGCGTGTACTAAGTTACTTTGATGAAGACGGTGATGGAAAGATTTCACCGAACGAGTTAAGGAGTCGAATGACGAAGATTGGCGGAGAGTTTCAATTGAAAGAAGTGGAAATTGCTATTGAGGCATTGGATTCTGATGGTGATGGGCTATTGAGTTTGGAGGATTTAATAACGCTTATGGAATCCGGGGGAGAAGAAGAAAAGTTGAAGGATTTGAGGGAAGCTTTTGAAATGTATGATAATGAAGGATGTGGGTTCATAACACCAAAGAGTTTGAAGAGGATGTTGAAAAAGTTGGGGGATTCTAAGTCCATTGAAGAATGTAAAGTGATGATTGGAAGGTTTGATTTGGATGGTGATGGTGTGCTTAGTTTTGAAGAATTCAGAATCATGATGGAGTGA

Protein sequence

>MS.gene011400.t1

MRSNMEFERVLSYFDEDGDGKISPNELRSRMTKIGGEFQLKEVEIAIEALDSDGDGLLSLEDLITLMESGGEEEKLKDLREAFEMYDNEGCGFITPKSLKRMLKKLGDSKSIEECKVMIGRFDLDGDGVLSFEEFRIMME