AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63000


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63000.t1 MTR_1g030440 92.857 154 10 1 1 154 15 167 3.27e-97 277
MS.gene63000.t1 MTR_3g088655 64.968 157 48 3 1 150 1 157 6.78e-58 177
MS.gene63000.t1 MTR_4g067270 38.255 149 73 4 7 147 4 141 1.21e-21 85.5
MS.gene63000.t1 MTR_8g105230 38.462 143 71 3 8 144 36 167 3.75e-21 85.1
MS.gene63000.t1 MTR_7g074240 35.333 150 88 3 9 150 25 173 1.32e-20 83.6
MS.gene63000.t1 MTR_3g067610 34.667 150 77 3 4 147 1 135 2.10e-20 82.0
MS.gene63000.t1 MTR_1g046950 37.415 147 77 3 7 147 3 140 4.17e-19 78.6
MS.gene63000.t1 MTR_5g079340 36.054 147 76 3 7 147 3 137 1.15e-18 77.4
MS.gene63000.t1 MTR_6g023460 37.063 143 72 3 12 148 12 142 1.18e-17 74.7
MS.gene63000.t1 MTR_2g098890 36.913 149 77 3 7 147 63 202 2.71e-17 75.5
MS.gene63000.t1 MTR_1g032070 35.374 147 78 3 8 148 46 181 2.91e-17 75.1
MS.gene63000.t1 MTR_1g047100 34.014 147 81 2 7 147 3 139 4.25e-17 73.6
MS.gene63000.t1 MTR_7g089760 34.868 152 80 3 4 147 1 141 6.07e-16 70.5
MS.gene63000.t1 MTR_6g079570 35.065 154 79 5 7 152 25 165 9.29e-16 70.9
MS.gene63000.t1 MTR_4g086260 31.081 148 95 1 7 154 56 196 9.31e-16 71.2
MS.gene63000.t1 MTR_4g115170 35.669 157 84 3 6 147 62 216 1.85e-15 70.9
MS.gene63000.t1 MTR_8g066630 32.432 148 84 3 7 147 16 154 1.99e-15 69.7
MS.gene63000.t1 MTR_8g107110 33.333 147 80 3 7 147 23 157 3.63e-15 68.9
MS.gene63000.t1 MTR_5g092810 32.450 151 83 4 9 153 398 535 5.30e-15 71.2
MS.gene63000.t1 MTR_7g075040 37.086 151 79 6 7 147 46 190 1.17e-14 68.2
MS.gene63000.t1 MTR_4g112460 31.250 144 90 2 7 147 10 147 2.29e-14 67.0
MS.gene63000.t1 MTR_8g036075 33.117 154 88 4 8 152 49 196 5.07e-14 66.6
MS.gene63000.t1 MTR_0028s0170 31.034 145 81 4 9 147 384 515 5.51e-14 68.6
MS.gene63000.t1 MTR_5g009830 32.414 145 79 3 9 147 372 503 7.03e-14 68.2
MS.gene63000.t1 MTR_4g132070 31.724 145 80 4 9 147 397 528 9.47e-14 67.8
MS.gene63000.t1 MTR_8g099095 32.414 145 79 4 9 147 372 503 1.70e-13 67.0
MS.gene63000.t1 MTR_4g103650 31.507 146 82 3 8 147 2 135 1.94e-13 63.5
MS.gene63000.t1 MTR_5g017510 33.103 145 88 4 6 148 46 183 2.35e-13 64.7
MS.gene63000.t1 MTR_5g017560 33.103 145 88 4 6 148 46 183 2.35e-13 64.7
MS.gene63000.t1 MTR_5g017550 30.872 149 85 5 6 148 46 182 4.05e-13 63.9
MS.gene63000.t1 MTR_3g083470 31.469 143 83 4 7 149 92 219 5.16e-13 64.3
MS.gene63000.t1 MTR_5g089320 30.345 145 82 4 9 147 374 505 8.96e-13 65.1
MS.gene63000.t1 MTR_3g051770 31.126 151 80 4 9 147 389 527 1.02e-12 64.7
MS.gene63000.t1 MTR_1g054865 31.690 142 83 3 9 147 467 597 1.37e-12 64.7
MS.gene63000.t1 MTR_4g103655 32.143 140 89 1 8 147 6 139 1.42e-12 61.6
MS.gene63000.t1 MTR_4g103630 30.137 146 84 3 8 147 6 139 3.48e-12 60.5
MS.gene63000.t1 MTR_7g090450 34.615 156 80 6 8 153 73 216 4.10e-12 61.6
MS.gene63000.t1 MTR_1g076650 29.577 142 83 3 13 148 17 147 7.72e-12 59.7
MS.gene63000.t1 MTR_6g025320 29.333 150 85 5 7 148 11 147 8.96e-12 59.7
MS.gene63000.t1 MTR_5g088320 29.333 150 85 5 7 148 11 147 1.01e-11 59.7
MS.gene63000.t1 MTR_1g026190 29.496 139 91 2 9 147 388 519 1.09e-11 62.0
MS.gene63000.t1 MTR_7g087610 29.333 150 85 5 7 148 11 147 1.35e-11 59.3
MS.gene63000.t1 MTR_8g069915 30.714 140 94 2 8 147 12 148 1.50e-11 59.3
MS.gene63000.t1 MTR_1g041285 30.872 149 82 4 8 148 12 147 1.88e-11 58.9
MS.gene63000.t1 MTR_3g098090 31.655 139 87 2 9 147 338 468 4.37e-11 60.1
MS.gene63000.t1 MTR_4g132040 32.414 145 79 4 9 147 401 532 6.17e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63000.t1 AT1G21550 46.358 151 72 5 4 148 6 153 1.41e-32 113
MS.gene63000.t1 AT4G20780 40.909 154 76 4 9 147 30 183 9.11e-26 97.1
MS.gene63000.t1 AT2G43290 36.913 149 80 3 7 147 64 206 6.20e-22 87.8
MS.gene63000.t1 AT1G24620 38.158 152 77 3 8 153 37 177 1.83e-21 85.9
MS.gene63000.t1 AT5G44460 41.781 146 78 3 9 147 29 174 8.32e-21 84.0
MS.gene63000.t1 AT1G05990 35.570 149 78 3 7 147 4 142 6.35e-19 78.6
MS.gene63000.t1 AT3G59440 35.135 148 76 3 8 147 51 186 7.76e-18 76.6
MS.gene63000.t1 AT5G37770 32.237 152 90 2 2 147 11 155 7.87e-18 75.9
MS.gene63000.t1 AT5G37770 32.237 152 90 2 2 147 11 155 7.87e-18 75.9
MS.gene63000.t1 AT1G76640 34.932 146 80 2 8 147 22 158 4.46e-17 73.9
MS.gene63000.t1 AT4G03290 33.557 149 83 3 7 147 4 144 7.90e-17 73.2
MS.gene63000.t1 AT4G12860 32.895 152 83 3 4 147 1 141 9.65e-17 72.8
MS.gene63000.t1 AT1G66400 30.323 155 93 2 2 150 9 154 2.22e-16 72.0
MS.gene63000.t1 AT1G73630 32.414 145 89 1 3 147 15 150 2.23e-16 72.0
MS.gene63000.t1 AT4G04710 34.483 145 76 4 9 147 338 469 1.27e-15 73.2
MS.gene63000.t1 AT4G04710 34.483 145 76 4 9 147 324 455 1.34e-15 73.2
MS.gene63000.t1 AT4G04710 34.483 145 76 4 9 147 351 482 1.34e-15 73.2
MS.gene63000.t1 AT1G32250 32.653 147 88 2 7 147 15 156 1.60e-15 70.1
MS.gene63000.t1 AT4G04710 34.014 147 76 4 9 147 351 484 2.75e-15 72.4
MS.gene63000.t1 AT3G07490 34.211 152 81 3 4 147 1 141 3.40e-15 68.9
MS.gene63000.t1 AT5G12180 32.414 145 79 4 9 147 379 510 3.69e-15 72.0
MS.gene63000.t1 AT4G23650 35.172 145 75 4 9 147 384 515 4.06e-15 71.6
MS.gene63000.t1 AT5G42380 36.111 144 88 1 4 147 45 184 5.47e-15 68.9
MS.gene63000.t1 AT5G19360 31.724 145 80 4 9 147 374 505 5.90e-15 71.2
MS.gene63000.t1 AT1G18530 34.028 144 84 3 8 147 7 143 1.85e-14 67.0
MS.gene63000.t1 AT1G76650 35.616 146 76 3 8 147 43 176 2.12e-14 67.4
MS.gene63000.t1 AT1G76650 35.616 146 76 3 8 147 43 176 2.12e-14 67.4
MS.gene63000.t1 AT5G17470 32.394 142 81 4 12 147 6 138 4.16e-14 65.9
MS.gene63000.t1 AT4G04695 32.414 145 79 4 9 147 337 468 5.63e-14 68.6
MS.gene63000.t1 AT4G04700 33.103 145 78 4 9 147 337 468 8.78e-14 67.8
MS.gene63000.t1 AT2G15680 31.206 141 90 1 7 147 49 182 1.27e-13 65.5
MS.gene63000.t1 AT2G41100 30.070 143 87 3 13 143 17 158 4.37e-13 65.5
MS.gene63000.t1 AT2G41100 30.070 143 87 3 13 143 17 158 4.37e-13 65.5
MS.gene63000.t1 AT1G18210 31.081 148 77 2 8 147 23 153 7.05e-13 63.2
MS.gene63000.t1 AT1G18210 31.081 148 77 2 8 147 23 153 7.05e-13 63.2
MS.gene63000.t1 AT1G76650 35.000 140 75 3 8 147 43 166 7.89e-13 62.8
MS.gene63000.t1 AT4G04720 31.206 141 86 3 9 147 385 516 1.12e-12 64.7
MS.gene63000.t1 AT4G04720 31.206 141 86 3 9 147 385 516 1.12e-12 64.7
MS.gene63000.t1 AT4G21940 31.724 145 80 4 9 147 407 538 1.68e-12 64.3
MS.gene63000.t1 AT4G21940 31.724 145 80 4 9 147 407 538 1.68e-12 64.3
MS.gene63000.t1 AT1G50700 28.966 145 84 4 9 147 379 510 2.28e-12 63.9
MS.gene63000.t1 AT4G04740 31.206 141 86 3 9 147 374 505 4.25e-12 63.2
MS.gene63000.t1 AT4G04740 31.206 141 86 3 9 147 374 505 4.25e-12 63.2
MS.gene63000.t1 AT4G04740 31.206 141 86 3 9 147 374 505 4.25e-12 63.2
MS.gene63000.t1 AT4G04740 31.206 141 86 3 9 147 374 505 4.25e-12 63.2
MS.gene63000.t1 AT4G04740 31.206 141 86 3 9 147 374 505 4.25e-12 63.2
MS.gene63000.t1 AT4G04740 31.206 141 86 3 9 147 374 505 4.25e-12 63.2
MS.gene63000.t1 AT1G35670 32.414 145 78 4 9 147 332 462 4.86e-12 62.8
MS.gene63000.t1 AT2G36180 32.867 143 80 4 12 147 4 137 5.59e-12 60.1
MS.gene63000.t1 AT2G38910 31.655 139 87 2 9 147 440 570 6.03e-12 62.8
MS.gene63000.t1 AT3G50770 31.333 150 93 4 5 149 61 205 7.65e-12 60.8
MS.gene63000.t1 AT3G20410 29.655 145 83 4 9 147 397 528 8.01e-12 62.4
MS.gene63000.t1 AT3G20410 29.655 145 83 4 9 147 397 528 8.01e-12 62.4
MS.gene63000.t1 AT3G43810 29.333 150 85 5 7 148 11 147 9.38e-12 59.7
MS.gene63000.t1 AT3G56800 29.333 150 85 5 7 148 11 147 1.21e-11 59.3
MS.gene63000.t1 AT2G27030 29.333 150 85 5 7 148 11 147 1.21e-11 59.3
MS.gene63000.t1 AT2G41110 29.333 150 85 5 7 148 11 147 1.21e-11 59.3
MS.gene63000.t1 AT5G21274 29.333 150 85 5 7 148 11 147 1.26e-11 59.3
MS.gene63000.t1 AT2G27030 29.333 150 85 5 7 148 11 147 1.43e-11 59.7
MS.gene63000.t1 AT5G37780 26.351 148 92 2 7 148 11 147 1.43e-11 59.3
MS.gene63000.t1 AT1G66410 26.351 148 92 2 7 148 11 147 1.43e-11 59.3
MS.gene63000.t1 AT3G43810 28.667 150 86 4 7 148 11 147 1.70e-11 60.1
MS.gene63000.t1 AT4G09570 32.414 145 78 4 9 147 331 461 2.27e-11 60.8
MS.gene63000.t1 AT3G51850 31.507 146 82 3 8 147 359 492 3.25e-11 60.5
MS.gene63000.t1 AT1G61950 30.345 145 82 4 9 147 405 536 5.77e-11 59.7
MS.gene63000.t1 AT1G50700 28.302 159 93 5 9 147 379 536 7.40e-11 59.7
MS.gene63000.t1 AT1G76040 29.577 142 87 3 9 147 417 548 8.51e-11 59.3

Find 25 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATATGATATTAATTTAGA+TGG 0.283864 1.3:-16331158 MS.gene63000:CDS
TTCAAGGTGTTTGATTTAGA+TGG 0.287480 1.3:-16331272 MS.gene63000:CDS
TCCCCTAACCCGCAATGAAT+TGG 0.338232 1.3:-16331537 MS.gene63000:CDS
CTCCAATTCATTGCGGGTTA+GGG 0.340726 1.3:+16331535 None:intergenic
GCTCCAATTCATTGCGGGTT+AGG 0.348062 1.3:+16331534 None:intergenic
AATCAATTGTTAGTGAGAAC+AGG 0.403944 1.3:-16331455 MS.gene63000:CDS
AGATGGCAAGCTTGATTTCA+AGG 0.404957 1.3:-16331141 MS.gene63000:CDS
TATCAGCTCCAATTCATTGC+GGG 0.425822 1.3:+16331529 None:intergenic
TGGTATGTGGGATGAAGAAA+AGG 0.481114 1.3:-16331201 MS.gene63000:CDS
GTGTTTGATTTAGATGGAGA+TGG 0.487484 1.3:-16331266 MS.gene63000:CDS
ACACCTTGAAAGCCTTCAAA+AGG 0.524154 1.3:+16331285 None:intergenic
ATTCATCACTAGTCAAGAGC+TGG 0.543089 1.3:-16331243 MS.gene63000:CDS
ACAATTGATTCAGCTCCTCA+AGG 0.548233 1.3:+16331468 None:intergenic
AAACTAGACATAAATAGTGA+TGG 0.557379 1.3:-16331500 MS.gene63000:CDS
TGTTGAAGAGACTTGGTATG+TGG 0.566871 1.3:-16331214 MS.gene63000:CDS
TCCAATTCATTGCGGGTTAG+GGG 0.573279 1.3:+16331536 None:intergenic
CAATTGATTCAGCTCCTCAA+GGG 0.587040 1.3:+16331469 None:intergenic
GTTGAAGAGACTTGGTATGT+GGG 0.589453 1.3:-16331213 MS.gene63000:CDS
AGAGGAGCTTGAATATCTAG+TGG 0.598842 1.3:-16331417 MS.gene63000:CDS
TGATGGTGGTGATGAGCTTG+AGG 0.604530 1.3:-16331327 MS.gene63000:CDS
AGGTTTCAAGTATAGCATAG+AGG 0.617626 1.3:-16331435 MS.gene63000:CDS
GAGGAGCTTGAATATCTAGT+GGG 0.620423 1.3:-16331416 MS.gene63000:CDS
GAATGTGTGTTGAAGAGACT+TGG 0.625496 1.3:-16331221 MS.gene63000:CDS
ATATCAGCTCCAATTCATTG+CGG 0.652735 1.3:+16331528 None:intergenic
TGGTGGTGATGAGCTTGAGG+AGG 0.681686 1.3:-16331324 MS.gene63000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATATGATATTAATTTAGA+TGG - chr1.3:16331484-16331503 MS.gene63000:CDS 15.0%
!! TCAATATTGAATCACAAAAA+TGG - chr1.3:16331292-16331311 MS.gene63000:CDS 20.0%
! AAACTAGACATAAATAGTGA+TGG - chr1.3:16331142-16331161 MS.gene63000:CDS 25.0%
AATCAATTGTTAGTGAGAAC+AGG - chr1.3:16331187-16331206 MS.gene63000:CDS 30.0%
TTGAATCACAAAAATGGTGA+TGG - chr1.3:16331298-16331317 MS.gene63000:CDS 30.0%
! ATTAGAAAGTGACCTTTTGA+AGG - chr1.3:16331345-16331364 MS.gene63000:CDS 30.0%
! TTCAAGGTGTTTGATTTAGA+TGG - chr1.3:16331370-16331389 MS.gene63000:CDS 30.0%
ATATCAGCTCCAATTCATTG+CGG + chr1.3:16331117-16331136 None:intergenic 35.0%
! AGGTTTCAAGTATAGCATAG+AGG - chr1.3:16331207-16331226 MS.gene63000:CDS 35.0%
! GTGTTTGATTTAGATGGAGA+TGG - chr1.3:16331376-16331395 MS.gene63000:CDS 35.0%
! TGATGGAATTTTATCCCTTG+AGG - chr1.3:16331159-16331178 MS.gene63000:CDS 35.0%
!! AATCACAAAAATGGTGATGG+TGG - chr1.3:16331301-16331320 MS.gene63000:CDS 35.0%
ACACCTTGAAAGCCTTCAAA+AGG + chr1.3:16331360-16331379 None:intergenic 40.0%
AGAGGAGCTTGAATATCTAG+TGG - chr1.3:16331225-16331244 MS.gene63000:CDS 40.0%
AGATGGCAAGCTTGATTTCA+AGG - chr1.3:16331501-16331520 MS.gene63000:CDS 40.0%
ATTCATCACTAGTCAAGAGC+TGG - chr1.3:16331399-16331418 MS.gene63000:CDS 40.0%
GAATGTGTGTTGAAGAGACT+TGG - chr1.3:16331421-16331440 MS.gene63000:CDS 40.0%
GAGGAGCTTGAATATCTAGT+GGG - chr1.3:16331226-16331245 MS.gene63000:CDS 40.0%
TATCAGCTCCAATTCATTGC+GGG + chr1.3:16331116-16331135 None:intergenic 40.0%
TGGTATGTGGGATGAAGAAA+AGG - chr1.3:16331441-16331460 MS.gene63000:CDS 40.0%
! ACAATTGATTCAGCTCCTCA+AGG + chr1.3:16331177-16331196 None:intergenic 40.0%
! CAATTGATTCAGCTCCTCAA+GGG + chr1.3:16331176-16331195 None:intergenic 40.0%
! GTTGAAGAGACTTGGTATGT+GGG - chr1.3:16331429-16331448 MS.gene63000:CDS 40.0%
! TGTTGAAGAGACTTGGTATG+TGG - chr1.3:16331428-16331447 MS.gene63000:CDS 40.0%
!! TGACCTTTTGAAGGCTTTCA+AGG - chr1.3:16331354-16331373 MS.gene63000:CDS 40.0%
! CTCCAATTCATTGCGGGTTA+GGG + chr1.3:16331110-16331129 None:intergenic 45.0%
!! TCCAATTCATTGCGGGTTAG+GGG + chr1.3:16331109-16331128 None:intergenic 45.0%
GCTCCAATTCATTGCGGGTT+AGG + chr1.3:16331111-16331130 None:intergenic 50.0%
TCCCCTAACCCGCAATGAAT+TGG - chr1.3:16331105-16331124 MS.gene63000:CDS 50.0%
!! TGATGGTGGTGATGAGCTTG+AGG - chr1.3:16331315-16331334 MS.gene63000:CDS 50.0%
! TGGTGGTGATGAGCTTGAGG+AGG - chr1.3:16331318-16331337 MS.gene63000:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 16331100 16331564 16331100 ID=MS.gene63000
chr1.3 mRNA 16331100 16331564 16331100 ID=MS.gene63000.t1;Parent=MS.gene63000
chr1.3 exon 16331100 16331564 16331100 ID=MS.gene63000.t1.exon1;Parent=MS.gene63000.t1
chr1.3 CDS 16331100 16331564 16331100 ID=cds.MS.gene63000.t1;Parent=MS.gene63000.t1
Gene Sequence

>MS.gene63000

ATGTGTCCCCTAACCCGCAATGAATTGGAGCTGATATTTAAGAAACTAGACATAAATAGTGATGGAATTTTATCCCTTGAGGAGCTGAATCAATTGTTAGTGAGAACAGGTTTCAAGTATAGCATAGAGGAGCTTGAATATCTAGTGGGAAAGAAGTGTCTAGACTTGAGTGAGTTCTTGTGCTTTTATGACTCAATATTGAATCACAAAAATGGTGATGGTGGTGATGAGCTTGAGGAGGTAGAATTAGAAAGTGACCTTTTGAAGGCTTTCAAGGTGTTTGATTTAGATGGAGATGGATTCATCACTAGTCAAGAGCTGGAATGTGTGTTGAAGAGACTTGGTATGTGGGATGAAGAAAAGGATTGTAGATCAATGATTTACTCATATGATATTAATTTAGATGGCAAGCTTGATTTCAAGGAATTCAAGAACATGATGTTGCTTACACTACAACGTGTTTAG

Protein sequence

>MS.gene63000.t1

MCPLTRNELELIFKKLDINSDGILSLEELNQLLVRTGFKYSIEELEYLVGKKCLDLSEFLCFYDSILNHKNGDGGDELEEVELESDLLKAFKVFDLDGDGFITSQELECVLKRLGMWDEEKDCRSMIYSYDINLDGKLDFKEFKNMMLLTLQRV