AlfalfaGEDB | MS.gene025797

M. sativa cultivar XinJiangDaYe / MS.gene025797

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF00036.35 PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025797.t1	XP_003611244.1	97.8	135	3	0	1	135	13	147	5.60E-68	266.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025797.t1	Q94AZ4	86.7	135	18	0	1	135	14	148	4.6e-63	241.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025797.t1	B7FNA2	97.8	135	3	0	1	135	13	147	4.0e-68	266.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene056383	MS.gene025797	PPI
MS.gene64324	MS.gene025797	PPI
MS.gene022455	MS.gene025797	PPI
MS.gene015470	MS.gene025797	PPI
MS.gene041795	MS.gene025797	PPI
MS.gene63000	MS.gene025797	PPI
MS.gene22553	MS.gene025797	PPI
MS.gene53662	MS.gene025797	PPI
MS.gene025797	MS.gene64317	PPI
MS.gene057293	MS.gene025797	PPI
MS.gene96914	MS.gene025797	PPI
MS.gene53648	MS.gene025797	PPI
MS.gene033361	MS.gene025797	PPI
MS.gene025797	MS.gene29459	PPI
MS.gene53659	MS.gene025797	PPI
MS.gene038456	MS.gene025797	PPI
MS.gene004899	MS.gene025797	PPI
MS.gene96922	MS.gene025797	PPI
MS.gene009021	MS.gene025797	PPI
MS.gene47134	MS.gene025797	PPI
MS.gene46237	MS.gene025797	PPI
MS.gene025797	MS.gene21696	PPI
MS.gene87673	MS.gene025797	PPI
MS.gene98295	MS.gene025797	PPI
MS.gene29793	MS.gene025797	PPI
MS.gene05284	MS.gene025797	PPI
MS.gene21931	MS.gene025797	PPI
MS.gene074100	MS.gene025797	PPI
MS.gene046988	MS.gene025797	PPI
MS.gene025797	MS.gene64314	PPI
MS.gene64316	MS.gene025797	PPI
MS.gene63281	MS.gene025797	PPI
MS.gene29794	MS.gene025797	PPI
MS.gene68345	MS.gene025797	PPI
MS.gene017446	MS.gene025797	PPI
MS.gene89216	MS.gene025797	PPI
MS.gene001246	MS.gene025797	PPI
MS.gene040127	MS.gene025797	PPI
MS.gene62258	MS.gene025797	PPI
MS.gene64310	MS.gene025797	PPI
MS.gene000724	MS.gene025797	PPI
MS.gene74452	MS.gene025797	PPI
MS.gene060446	MS.gene025797	PPI
MS.gene025797	MS.gene79028	PPI
MS.gene64311	MS.gene025797	PPI
MS.gene008831	MS.gene025797	PPI
MS.gene96913	MS.gene025797	PPI
MS.gene040331	MS.gene025797	PPI
MS.gene58906	MS.gene025797	PPI
MS.gene58860	MS.gene025797	PPI
MS.gene98294	MS.gene025797	PPI
MS.gene27516	MS.gene025797	PPI
MS.gene02545	MS.gene025797	PPI
MS.gene053319	MS.gene025797	PPI
MS.gene04915	MS.gene025797	PPI
MS.gene49270	MS.gene025797	PPI
MS.gene61009	MS.gene025797	PPI
MS.gene64327	MS.gene025797	PPI
MS.gene000738	MS.gene025797	PPI
MS.gene64307	MS.gene025797	PPI
MS.gene061261	MS.gene025797	PPI
MS.gene026618	MS.gene025797	PPI
MS.gene04917	MS.gene025797	PPI
MS.gene025797	MS.gene09960	PPI
MS.gene074045	MS.gene025797	PPI
MS.gene47195	MS.gene025797	PPI
MS.gene025797	MS.gene061768	PPI
MS.gene036930	MS.gene025797	PPI
MS.gene013748	MS.gene025797	PPI
MS.gene025797	MS.gene53650	PPI
MS.gene55545	MS.gene025797	PPI
MS.gene65954	MS.gene025797	PPI
MS.gene008225	MS.gene025797	PPI
MS.gene025797	MS.gene057167	PPI
MS.gene025797	MS.gene93763	PPI
MS.gene011400	MS.gene025797	PPI
MS.gene09960	MS.gene025797	PPI
MS.gene024717	MS.gene025797	PPI
MS.gene64308	MS.gene025797	PPI
MS.gene64313	MS.gene025797	PPI
MS.gene93087	MS.gene025797	PPI
MS.gene35563	MS.gene025797	PPI
MS.gene97948	MS.gene025797	PPI
MS.gene55496	MS.gene025797	PPI
MS.gene003880	MS.gene025797	PPI
MS.gene54637	MS.gene025797	PPI
MS.gene49271	MS.gene025797	PPI
MS.gene67281	MS.gene025797	PPI
MS.gene025797	MS.gene71511	PPI
MS.gene62540	MS.gene025797	PPI
MS.gene025777	MS.gene025797	PPI
MS.gene025797	MS.gene056461	PPI
MS.gene022456	MS.gene025797	PPI
MS.gene062239	MS.gene025797	PPI
MS.gene46886	MS.gene025797	PPI
MS.gene025797	MS.gene35562	PPI
MS.gene026940	MS.gene025797	PPI
MS.gene21155	MS.gene025797	PPI
MS.gene71207	MS.gene025797	PPI
MS.gene92775	MS.gene025797	PPI
MS.gene07422	MS.gene025797	PPI
MS.gene03228	MS.gene025797	PPI
MS.gene09934	MS.gene025797	PPI
MS.gene21932	MS.gene025797	PPI
MS.gene28338	MS.gene025797	PPI
MS.gene85596	MS.gene025797	PPI
MS.gene94551	MS.gene025797	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025797.t1	MTR_5g011850	97.778	135	3	0	1	135	13	147	8.70e-95	270
MS.gene025797.t1	MTR_5g088320	50.735	136	65	1	2	135	14	149	7.78e-44	141
MS.gene025797.t1	MTR_6g025320	50.735	136	65	1	2	135	14	149	1.06e-43	141
MS.gene025797.t1	MTR_7g087610	50.000	136	66	1	2	135	14	149	1.64e-43	140
MS.gene025797.t1	MTR_1g041285	42.647	136	76	1	1	134	13	148	4.91e-36	122
MS.gene025797.t1	MTR_1g076650	40.741	135	78	1	2	134	14	148	1.18e-34	118
MS.gene025797.t1	MTR_0168s0120	48.673	113	56	1	25	135	1	113	1.70e-33	114
MS.gene025797.t1	MTR_6g025320	48.673	113	56	1	25	135	1	113	1.70e-33	114
MS.gene025797.t1	MTR_1g071150	35.714	140	82	3	3	140	15	148	2.35e-26	97.4
MS.gene025797.t1	MTR_7g115040	37.313	134	82	1	3	134	15	148	1.78e-22	87.4
MS.gene025797.t1	MTR_8g066630	34.586	133	82	3	1	128	18	150	1.83e-21	85.1
MS.gene025797.t1	MTR_1g032070	33.824	136	88	1	1	134	47	182	9.65e-20	81.6
MS.gene025797.t1	MTR_8g105230	33.846	130	84	1	1	128	37	166	8.04e-19	79.0
MS.gene025797.t1	MTR_7g074020	31.343	134	90	1	1	132	31	164	1.81e-18	77.4
MS.gene025797.t1	MTR_6g007613	31.343	134	90	1	1	132	30	163	6.10e-18	76.3
MS.gene025797.t1	MTR_5g079340	33.824	136	85	3	1	133	5	138	4.82e-17	73.2
MS.gene025797.t1	MTR_4g107490	33.582	134	86	2	1	132	379	511	7.36e-17	76.6
MS.gene025797.t1	MTR_4g112460	31.618	136	89	2	1	132	12	147	1.22e-16	72.8
MS.gene025797.t1	MTR_7g074020	31.356	118	79	1	1	116	31	148	2.00e-16	72.0
MS.gene025797.t1	MTR_8g107110	33.588	131	82	2	1	128	25	153	3.22e-16	71.6
MS.gene025797.t1	MTR_6g007613	31.356	118	79	1	1	116	30	147	6.03e-16	70.9
MS.gene025797.t1	MTR_1g096490	30.597	134	88	3	1	132	336	466	2.66e-15	72.0
MS.gene025797.t1	MTR_3g055570	36.842	133	63	3	1	129	53	168	5.85e-15	68.6
MS.gene025797.t1	MTR_1g046950	30.657	137	91	2	1	133	5	141	6.29e-15	67.8
MS.gene025797.t1	MTR_6g079570	32.624	141	85	4	1	135	27	163	7.70e-15	68.2
MS.gene025797.t1	MTR_1g047100	29.197	137	94	2	1	134	5	141	1.54e-14	66.6
MS.gene025797.t1	MTR_1g055255	32.836	134	85	3	1	132	430	560	3.66e-14	68.9
MS.gene025797.t1	MTR_7g106710	29.851	134	89	3	1	132	441	571	4.68e-14	68.6
MS.gene025797.t1	MTR_3g051770	34.286	140	83	3	1	132	389	527	9.63e-14	67.8
MS.gene025797.t1	MTR_5g027000	27.737	137	95	2	3	137	25	159	9.83e-14	65.1
MS.gene025797.t1	MTR_3g067610	29.231	130	86	2	1	128	6	131	2.94e-13	63.2
MS.gene025797.t1	MTR_7g068710	31.343	134	89	2	1	132	362	494	3.30e-13	66.2
MS.gene025797.t1	MTR_4g067270	30.882	136	90	2	1	132	6	141	4.96e-13	62.8
MS.gene025797.t1	MTR_1g041150	30.597	134	88	3	1	132	424	554	6.47e-13	65.5
MS.gene025797.t1	MTR_7g075040	31.690	142	86	3	2	132	49	190	1.95e-12	62.4
MS.gene025797.t1	MTR_1g054865	31.343	134	87	3	1	132	467	597	2.11e-12	63.9
MS.gene025797.t1	MTR_8g069915	25.161	155	109	3	1	148	13	167	3.18e-12	61.2
MS.gene025797.t1	MTR_8g095440	28.358	134	91	3	1	132	336	466	3.63e-12	63.2
MS.gene025797.t1	MTR_3g098070	27.612	134	92	3	1	132	335	465	4.22e-12	62.8
MS.gene025797.t1	MTR_3g098090	27.612	134	92	3	1	132	338	468	5.28e-12	62.8
MS.gene025797.t1	MTR_8g036075	31.915	141	86	3	2	132	51	191	6.56e-12	60.8
MS.gene025797.t1	MTR_1g052530	29.412	136	93	2	1	134	358	492	8.99e-12	62.0
MS.gene025797.t1	MTR_5g092810	31.429	140	92	3	1	138	398	535	1.09e-11	61.6
MS.gene025797.t1	MTR_3g083470	31.250	128	80	2	1	128	94	213	1.18e-11	60.5
MS.gene025797.t1	MTR_5g099240	28.358	134	91	3	1	132	402	532	1.89e-11	61.2
MS.gene025797.t1	MTR_7g091890	30.147	136	92	2	1	134	364	498	2.04e-11	60.8
MS.gene025797.t1	MTR_5g099240	28.358	134	91	3	1	132	330	460	2.08e-11	60.8
MS.gene025797.t1	MTR_4g103650	31.538	130	80	4	4	128	6	131	5.55e-11	57.0
MS.gene025797.t1	MTR_4g103655	31.538	130	80	4	4	128	10	135	5.57e-11	57.4
MS.gene025797.t1	MTR_4g132070	32.090	134	87	3	1	132	397	528	5.78e-11	59.7
MS.gene025797.t1	MTR_5g008705	26.923	130	93	1	7	134	1	130	7.19e-11	56.6
MS.gene025797.t1	MTR_4g115170	30.405	148	83	3	1	128	65	212	8.99e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025797.t1	AT1G12310	86.667	135	18	0	1	135	14	148	1.65e-84	244
MS.gene025797.t1	AT1G62820	83.704	135	22	0	1	135	14	148	7.25e-83	240
MS.gene025797.t1	AT5G21274	50.735	136	65	1	2	135	14	149	7.64e-44	141
MS.gene025797.t1	AT3G43810	50.735	136	65	1	2	135	14	149	1.11e-43	141
MS.gene025797.t1	AT5G37780	50.735	136	65	1	2	135	14	149	2.30e-43	140
MS.gene025797.t1	AT1G66410	50.735	136	65	1	2	135	14	149	2.30e-43	140
MS.gene025797.t1	AT3G56800	50.000	136	66	1	2	135	14	149	3.33e-43	140
MS.gene025797.t1	AT2G27030	50.000	136	66	1	2	135	14	149	3.33e-43	140
MS.gene025797.t1	AT2G41110	50.000	136	66	1	2	135	14	149	3.33e-43	140
MS.gene025797.t1	AT2G27030	50.000	136	66	1	2	135	14	149	6.30e-43	140
MS.gene025797.t1	AT3G43810	49.275	138	68	1	2	137	14	151	1.88e-42	140
MS.gene025797.t1	AT2G41110	45.946	148	66	2	2	135	14	161	1.42e-39	131
MS.gene025797.t1	AT5G37780	45.695	151	65	2	2	135	14	164	4.18e-39	130
MS.gene025797.t1	AT5G37780	42.593	162	65	2	2	135	14	175	4.21e-37	125
MS.gene025797.t1	AT1G66410	47.260	146	65	2	2	135	14	159	6.23e-37	124
MS.gene025797.t1	AT5G37780	48.387	124	62	1	14	135	13	136	2.24e-36	122
MS.gene025797.t1	AT3G22930	41.791	134	76	1	2	133	37	170	8.81e-36	122
MS.gene025797.t1	AT4G14640	40.299	134	78	1	2	133	15	148	1.74e-34	118
MS.gene025797.t1	AT3G43810	48.673	113	56	1	25	135	1	113	1.79e-33	114
MS.gene025797.t1	AT3G43810	48.673	113	56	1	25	135	1	113	1.79e-33	114
MS.gene025797.t1	AT1G66410	48.673	113	56	1	25	135	1	113	4.09e-33	113
MS.gene025797.t1	AT1G66410	48.673	113	56	1	25	135	1	113	4.09e-33	113
MS.gene025797.t1	AT4G14640	36.885	122	75	1	14	133	9	130	1.43e-27	100
MS.gene025797.t1	AT2G41100	35.714	168	86	4	2	150	68	232	5.94e-25	97.1
MS.gene025797.t1	AT2G41100	29.688	128	72	3	24	133	1	128	2.56e-11	60.5
MS.gene025797.t1	AT2G41100	35.714	168	86	4	2	150	68	232	5.94e-25	97.1
MS.gene025797.t1	AT2G41100	29.688	128	72	3	24	133	1	128	2.56e-11	60.5
MS.gene025797.t1	AT2G41100	35.714	168	86	4	2	150	68	232	5.94e-25	97.1
MS.gene025797.t1	AT2G41100	29.688	128	72	3	24	133	1	128	2.56e-11	60.5
MS.gene025797.t1	AT2G41100	35.714	168	86	4	2	150	103	267	9.63e-25	97.1
MS.gene025797.t1	AT2G41100	32.667	150	83	3	2	133	14	163	2.36e-19	82.8
MS.gene025797.t1	AT2G41100	35.714	168	86	4	2	150	103	267	9.63e-25	97.1
MS.gene025797.t1	AT2G41100	32.667	150	83	3	2	133	14	163	2.36e-19	82.8
MS.gene025797.t1	AT2G41090	36.765	136	81	2	2	135	14	146	8.56e-22	86.7
MS.gene025797.t1	AT4G14640	42.553	94	52	1	2	93	15	108	2.01e-21	84.0
MS.gene025797.t1	AT3G22930	41.935	93	52	1	2	92	37	129	1.05e-20	82.4
MS.gene025797.t1	AT3G50360	31.343	134	90	1	1	132	28	161	1.40e-19	80.5
MS.gene025797.t1	AT4G37010	32.836	134	88	1	1	132	28	161	8.06e-19	78.6
MS.gene025797.t1	AT4G37010	32.836	134	88	1	1	132	32	165	8.08e-19	78.6
MS.gene025797.t1	AT2G41100	34.483	145	73	4	25	150	1	142	2.75e-18	77.8
MS.gene025797.t1	AT3G51920	31.343	134	90	1	3	134	15	148	5.58e-18	75.9
MS.gene025797.t1	AT1G24620	32.867	143	94	1	1	141	38	180	6.28e-18	76.6
MS.gene025797.t1	AT1G32250	30.882	136	86	3	1	128	17	152	1.83e-17	74.7
MS.gene025797.t1	AT1G74740	35.766	137	83	4	1	134	365	499	3.11e-16	74.7
MS.gene025797.t1	AT1G18530	32.374	139	89	3	1	135	8	145	3.76e-15	68.6
MS.gene025797.t1	AT1G35670	32.090	134	86	3	1	132	332	462	5.01e-15	71.2
MS.gene025797.t1	AT1G76040	33.582	134	85	3	1	132	179	310	5.31e-15	70.9
MS.gene025797.t1	AT1G76040	33.582	134	85	3	1	132	179	310	5.31e-15	70.9
MS.gene025797.t1	AT3G03000	32.847	137	89	2	1	134	21	157	6.22e-15	68.2
MS.gene025797.t1	AT1G76040	33.582	134	85	3	1	132	417	548	6.26e-15	71.2
MS.gene025797.t1	AT4G09570	31.343	134	87	3	1	132	331	461	2.40e-14	69.3
MS.gene025797.t1	AT1G18210	29.323	133	92	1	1	133	24	154	3.27e-14	66.6
MS.gene025797.t1	AT1G18210	29.323	133	92	1	1	133	24	154	3.27e-14	66.6
MS.gene025797.t1	AT1G66400	30.657	137	91	2	1	133	16	152	4.04e-14	65.9
MS.gene025797.t1	AT5G23580	29.851	134	89	3	1	132	328	458	3.67e-13	65.9
MS.gene025797.t1	AT4G38230	29.104	134	90	3	1	132	360	490	7.57e-13	65.1
MS.gene025797.t1	AT4G38230	29.104	134	90	3	1	132	330	460	7.69e-13	65.1
MS.gene025797.t1	AT4G38230	29.104	134	90	3	1	132	330	460	7.69e-13	65.1
MS.gene025797.t1	AT4G38230	28.378	148	97	4	1	142	186	330	9.07e-13	64.7
MS.gene025797.t1	AT3G10660	31.343	134	87	3	1	132	492	622	1.15e-12	64.7
MS.gene025797.t1	AT3G10660	31.343	134	87	3	1	132	492	622	1.15e-12	64.7
MS.gene025797.t1	AT1G05990	30.827	133	87	3	1	128	6	138	1.26e-12	62.0
MS.gene025797.t1	AT3G59440	31.884	138	85	4	1	132	52	186	1.59e-12	62.4
MS.gene025797.t1	AT2G43290	29.577	142	89	4	1	132	66	206	3.08e-12	62.0
MS.gene025797.t1	AT3G20410	31.343	134	88	3	1	132	397	528	5.51e-12	62.8
MS.gene025797.t1	AT3G20410	31.343	134	88	3	1	132	397	528	5.51e-12	62.8
MS.gene025797.t1	AT2G17290	28.358	134	91	3	1	132	391	521	5.63e-12	62.8
MS.gene025797.t1	AT2G17290	28.358	134	91	3	1	132	391	521	5.63e-12	62.8
MS.gene025797.t1	AT2G15680	28.358	134	92	2	1	132	51	182	6.43e-12	60.8
MS.gene025797.t1	AT5G04870	29.851	134	89	3	1	132	456	586	7.52e-12	62.4
MS.gene025797.t1	AT2G38910	29.851	134	89	3	1	132	440	570	7.61e-12	62.4
MS.gene025797.t1	AT2G41860	30.597	134	90	2	1	132	360	492	8.07e-12	62.0
MS.gene025797.t1	AT4G03290	30.370	135	87	3	1	128	6	140	8.30e-12	59.7
MS.gene025797.t1	AT2G41860	30.597	134	90	2	1	132	360	492	8.47e-12	62.0
MS.gene025797.t1	AT1G73630	26.923	130	89	2	1	128	21	146	9.27e-12	59.7
MS.gene025797.t1	AT4G21940	32.090	134	87	3	1	132	407	538	1.14e-11	61.6
MS.gene025797.t1	AT4G21940	32.090	134	87	3	1	132	407	538	1.24e-11	61.6
MS.gene025797.t1	AT4G35310	29.104	134	90	3	1	132	403	533	2.64e-11	60.8
MS.gene025797.t1	AT4G35310	29.104	134	90	3	1	132	403	533	2.64e-11	60.8
MS.gene025797.t1	AT3G57530	30.935	139	82	4	1	132	369	500	3.24e-11	60.5
MS.gene025797.t1	AT1G18890	29.927	137	91	4	1	134	369	503	3.61e-11	60.5
MS.gene025797.t1	AT2G41100	32.500	80	51	1	71	150	12	88	3.88e-11	57.8
MS.gene025797.t1	AT4G23650	30.597	134	89	3	1	132	384	515	5.87e-11	59.7
MS.gene025797.t1	AT3G25600	26.846	149	102	3	1	142	13	161	6.65e-11	57.4
MS.gene025797.t1	AT5G12480	29.286	140	89	4	1	134	365	500	8.15e-11	59.3

Find 36 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTTCAGGTTTCATGTGTT+TGG	0.324663	5.3:+5437870	None:intergenic
CACATTGCATTGCATGTACT+TGG	0.334219	5.3:+5437648	None:intergenic
CTGAGTTTGATGAATGGATT+AGG	0.334899	5.3:-5437732	MS.gene025797:CDS
AGTTGATGATCGAACGGTTC+AGG	0.354968	5.3:+5437856	None:intergenic
TCAGATTTCTCAACAGAAAC+AGG	0.371452	5.3:-5436811	MS.gene025797:intron
GAAGTTGATGTTGGGTTTGA+TGG	0.388552	5.3:-5437709	MS.gene025797:CDS
CGATGGATTTGAGCTGAGCT+TGG	0.412141	5.3:+5437950	None:intergenic
TGCGATCTTGCCGTCGTTGT+CGG	0.415377	5.3:+5438020	None:intergenic
GGATTAGGGAAGTTGATGTT+GGG	0.442492	5.3:-5437717	MS.gene025797:CDS
TGGATTAGGGAAGTTGATGT+TGG	0.452504	5.3:-5437718	MS.gene025797:CDS
GTTCTCGATAAAGATGCAAC+TGG	0.460205	5.3:-5437817	MS.gene025797:CDS
ATTTGAGCTGAGCTTGGGTC+GGG	0.477294	5.3:+5437956	None:intergenic
AGCCTGCTGAGTTTGATGAA+TGG	0.478011	5.3:-5437738	MS.gene025797:CDS
TGAGTTTGATGAATGGATTA+GGG	0.481850	5.3:-5437731	MS.gene025797:CDS
AACCGTTCGATCATCAACTA+AGG	0.488078	5.3:-5437852	MS.gene025797:CDS
GATGGATTTGAGCTGAGCTT+GGG	0.498752	5.3:+5437951	None:intergenic
TGAAGATTTCATCGCTAGAA+TGG	0.506739	5.3:-5437674	MS.gene025797:CDS
AGGGGAGGTTAGATTCTCTT+CGG	0.513129	5.3:+5437924	None:intergenic
ATCCATTCATCAAACTCAGC+AGG	0.514616	5.3:+5437736	None:intergenic
TAGATTCTCTTCGGCGACGA+TGG	0.518627	5.3:+5437933	None:intergenic
GATTTGAGCTGAGCTTGGGT+CGG	0.523421	5.3:+5437955	None:intergenic
GGAATCCTCATGCGTTCTCT+CGG	0.535690	5.3:-5437985	MS.gene025797:CDS
AAGTTGATGTTGGGTTTGAT+GGG	0.546897	5.3:-5437708	MS.gene025797:CDS
CGACGGCAAGATCGCACCAT+CGG	0.559292	5.3:-5438013	MS.gene025797:CDS
CTCAACAGAAACAGGACAAG+TGG	0.561226	5.3:-5436803	MS.gene025797:CDS
GAAATCGAGAGAAATCGAAA+GGG	0.564111	5.3:+5437905	None:intergenic
AAGATCGCACCATCGGAGCT+CGG	0.573545	5.3:-5438006	MS.gene025797:CDS
AGAAATCGAGAGAAATCGAA+AGG	0.584540	5.3:+5437904	None:intergenic
TCCCTTAGTTGATGATCGAA+CGG	0.595563	5.3:+5437850	None:intergenic
ATGAGGATTCCGAGCTCCGA+TGG	0.601818	5.3:+5437997	None:intergenic
ATCCTCATGCGTTCTCTCGG+CGG	0.614038	5.3:-5437982	MS.gene025797:CDS
ACCGTTCGATCATCAACTAA+GGG	0.620555	5.3:-5437851	MS.gene025797:CDS
TACCGCCGAGAGAACGCATG+AGG	0.654227	5.3:+5437980	None:intergenic
CTCTTCGACACCGACAACGA+CGG	0.661849	5.3:-5438030	MS.gene025797:CDS
TCGAGAGAAATCGAAAGGGG+AGG	0.668937	5.3:+5437909	None:intergenic
AAATCGAGAGAAATCGAAAG+GGG	0.678072	5.3:+5437906	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTTAATGATATTTAATAAA+AGG	+	chr5.3:5437333-5437352	None:intergenic	10.0%
!!	ATTAAAAAAAAAGTGATAAT+CGG	+	chr5.3:5437709-5437728	None:intergenic	10.0%
!!	AATAAAAAAAAGGAAAATCT+AGG	+	chr5.3:5437556-5437575	None:intergenic	15.0%
!!!	ATTTTTGACAAAATAATAGT+TGG	-	chr5.3:5437406-5437425	MS.gene025797:intron	15.0%
!!!	CATAAATATCAATTCTAAAA+TGG	-	chr5.3:5437852-5437871	MS.gene025797:CDS	15.0%
!!	TTTATTCGAGATAGAAAAAT+TGG	+	chr5.3:5437375-5437394	None:intergenic	20.0%
!!!	AGCTTTAAATTTTTGTTTCA+TGG	-	chr5.3:5437486-5437505	MS.gene025797:intron	20.0%
!!!	ATTTGTTTGTGTTTTATACT+TGG	-	chr5.3:5437586-5437605	MS.gene025797:intron	20.0%
!	AAAAAAGGAAAATCTAGGTT+AGG	+	chr5.3:5437551-5437570	None:intergenic	25.0%
!	AAAAAGGAAAATCTAGGTTA+GGG	+	chr5.3:5437550-5437569	None:intergenic	25.0%
!	AAATTATGGGACCAATTTAA+CGG	-	chr5.3:5437617-5437636	MS.gene025797:intron	25.0%
!	ACTCAAAATTATTGAGTATC+AGG	-	chr5.3:5437902-5437921	MS.gene025797:CDS	25.0%
!	CTATCTCGAATAAATAGTTA+AGG	-	chr5.3:5437381-5437400	MS.gene025797:intron	25.0%
!	CTCAAAATTATTGAGTATCA+GGG	-	chr5.3:5437903-5437922	MS.gene025797:CDS	25.0%
!	CTTGGTGAAATAGAAATTAT+GGG	-	chr5.3:5437604-5437623	MS.gene025797:intron	25.0%
!	TAAAGCTTAAAACCTTATCT+TGG	+	chr5.3:5437473-5437492	None:intergenic	25.0%
!	TCAAAATTATTGAGTATCAG+GGG	-	chr5.3:5437904-5437923	MS.gene025797:CDS	25.0%
!	TTAGTATGAAAGATGAAAGT+AGG	-	chr5.3:5437816-5437835	MS.gene025797:CDS	25.0%
!!	ACTTGGTGAAATAGAAATTA+TGG	-	chr5.3:5437603-5437622	MS.gene025797:intron	25.0%
!!!	AATATGCACCCTTTTAAAAA+GGG	-	chr5.3:5437648-5437667	MS.gene025797:intron	25.0%
!!!	CTCAATAATTTTGAGTTTGA+CGG	+	chr5.3:5437898-5437917	None:intergenic	25.0%
!!!	TAATATGCACCCTTTTAAAA+AGG	-	chr5.3:5437647-5437666	MS.gene025797:intron	25.0%
!!!	TTTTAAAAGGGTGCATATTA+GGG	+	chr5.3:5437647-5437666	None:intergenic	25.0%
!!!	TTTTTAAAAGGGTGCATATT+AGG	+	chr5.3:5437648-5437667	None:intergenic	25.0%
	AAAACACAAACAAATTGCAC+TGG	+	chr5.3:5437581-5437600	None:intergenic	30.0%
	AATCTGCATCGAGATAAAAT+CGG	+	chr5.3:5437793-5437812	None:intergenic	30.0%
	AATTATGGGACCAATTTAAC+GGG	-	chr5.3:5437618-5437637	MS.gene025797:intron	30.0%
	ATAAAATCGGGACAATATTG+TGG	+	chr5.3:5437780-5437799	None:intergenic	30.0%
	CAAAAATAATCACAGACCAA+TGG	-	chr5.3:5437977-5437996	MS.gene025797:CDS	30.0%
	GCATTCATCTTAACTATTTG+TGG	+	chr5.3:5437512-5437531	None:intergenic	30.0%
!	TGCACTGGTCAATAAAAAAA+AGG	+	chr5.3:5437566-5437585	None:intergenic	30.0%
!	TTTAAAAGGGTGCATATTAG+GGG	+	chr5.3:5437646-5437665	None:intergenic	30.0%
!!	AAGATGAAAGTAGGAGTATT+TGG	-	chr5.3:5437825-5437844	MS.gene025797:CDS	30.0%
!!	CATTGGTCTGTGATTATTTT+TGG	+	chr5.3:5437979-5437998	None:intergenic	30.0%
!!	TGAGTTTGATGAATGGATTA+GGG	-	chr5.3:5437114-5437133	MS.gene025797:intron	30.0%
!!	TTGGTGTTGCATTCAATTAT+CGG	-	chr5.3:5437425-5437444	MS.gene025797:intron	30.0%
	AAAAATGAATGGTCCATGCT+TGG	+	chr5.3:5437675-5437694	None:intergenic	35.0%
	AAATCGAGAGAAATCGAAAG+GGG	+	chr5.3:5436942-5436961	None:intergenic	35.0%
	AAATTATTGAGTATCAGGGG+TGG	-	chr5.3:5437907-5437926	MS.gene025797:CDS	35.0%
	AAGTACATGCAATGCAATGT+GGG	-	chr5.3:5437199-5437218	MS.gene025797:intron	35.0%
	AGAAATCGAGAGAAATCGAA+AGG	+	chr5.3:5436944-5436963	None:intergenic	35.0%
	ATCCATGTACATAACACCAT+TGG	+	chr5.3:5437996-5438015	None:intergenic	35.0%
	ATCTGCATCGAGATAAAATC+GGG	+	chr5.3:5437792-5437811	None:intergenic	35.0%
	CTACTGTGGGTAAAAATGAA+TGG	+	chr5.3:5437686-5437705	None:intergenic	35.0%
	GAAATCGAGAGAAATCGAAA+GGG	+	chr5.3:5436943-5436962	None:intergenic	35.0%
	GTTGCATTCAATTATCGGAA+CGG	-	chr5.3:5437430-5437449	MS.gene025797:intron	35.0%
	TCAGATTTCTCAACAGAAAC+AGG	-	chr5.3:5438034-5438053	MS.gene025797:CDS	35.0%
	TGAAGATTTCATCGCTAGAA+TGG	-	chr5.3:5437171-5437190	MS.gene025797:intron	35.0%
!	ATGCTTGGACCCTTTTTAAA+AGG	+	chr5.3:5437660-5437679	None:intergenic	35.0%
!	CTGAGTTTGATGAATGGATT+AGG	-	chr5.3:5437113-5437132	MS.gene025797:intron	35.0%
!	TGGAACAAGATGTTCCATTT+TGG	+	chr5.3:5437959-5437978	None:intergenic	35.0%
!!	AAGTTGATGTTGGGTTTGAT+GGG	-	chr5.3:5437137-5437156	MS.gene025797:intron	35.0%
!!	GGTGTTGTTTGAAACCAAAA+TGG	-	chr5.3:5437942-5437961	MS.gene025797:CDS	35.0%
!!	TGCTTGGACCCTTTTTAAAA+GGG	+	chr5.3:5437659-5437678	None:intergenic	35.0%
!!	TTTTAAAAAGGGTCCAAGCA+TGG	-	chr5.3:5437659-5437678	MS.gene025797:CDS	35.0%
!!!	CGTCATATTTTGACGAGTAT+TGG	-	chr5.3:5437073-5437092	MS.gene025797:intron	35.0%
	AAAGTGATAATCGGCTACTG+TGG	+	chr5.3:5437700-5437719	None:intergenic	40.0%
	AACCGTTCGATCATCAACTA+AGG	-	chr5.3:5436993-5437012	MS.gene025797:intron	40.0%
	AAGTGATAATCGGCTACTGT+GGG	+	chr5.3:5437699-5437718	None:intergenic	40.0%
	ACCGTTCGATCATCAACTAA+GGG	-	chr5.3:5436994-5437013	MS.gene025797:intron	40.0%
	ATCCATTCATCAAACTCAGC+AGG	+	chr5.3:5437112-5437131	None:intergenic	40.0%
	ATCTTGGACTAATTGACCCT+TGG	+	chr5.3:5437457-5437476	None:intergenic	40.0%
	CAAGTACATGCAATGCAATG+TGG	-	chr5.3:5437198-5437217	MS.gene025797:intron	40.0%
	CACATTGCATTGCATGTACT+TGG	+	chr5.3:5437200-5437219	None:intergenic	40.0%
	GGGTCAATTAGTCCAAGATA+AGG	-	chr5.3:5437458-5437477	MS.gene025797:intron	40.0%
	GTTCTCGATAAAGATGCAAC+TGG	-	chr5.3:5437028-5437047	MS.gene025797:intron	40.0%
!!	GAAGTTGATGTTGGGTTTGA+TGG	-	chr5.3:5437136-5437155	MS.gene025797:intron	40.0%
!!	GACCAATGGTGTTATGTACA+TGG	-	chr5.3:5437991-5438010	MS.gene025797:CDS	40.0%
!!	GGATTAGGGAAGTTGATGTT+GGG	-	chr5.3:5437128-5437147	MS.gene025797:intron	40.0%
!!	TCCCTTAGTTGATGATCGAA+CGG	+	chr5.3:5436998-5437017	None:intergenic	40.0%
!!	TGGATTAGGGAAGTTGATGT+TGG	-	chr5.3:5437127-5437146	MS.gene025797:intron	40.0%
	AGCCTGCTGAGTTTGATGAA+TGG	-	chr5.3:5437107-5437126	MS.gene025797:intron	45.0%
	AGGGGAGGTTAGATTCTCTT+CGG	+	chr5.3:5436924-5436943	None:intergenic	45.0%
	CAATTATCGGAACGGTCCAA+GGG	-	chr5.3:5437438-5437457	MS.gene025797:intron	45.0%
	CTCAACAGAAACAGGACAAG+TGG	-	chr5.3:5438042-5438061	MS.gene025797:CDS	45.0%
	TCAATTATCGGAACGGTCCA+AGG	-	chr5.3:5437437-5437456	MS.gene025797:intron	45.0%
!	CGGTTCAGGTTTCATGTGTT+TGG	+	chr5.3:5436978-5436997	None:intergenic	45.0%
!	GATGGATTTGAGCTGAGCTT+GGG	+	chr5.3:5436897-5436916	None:intergenic	45.0%
!!	AGTTGATGATCGAACGGTTC+AGG	+	chr5.3:5436992-5437011	None:intergenic	45.0%
!!	ATTAGGGGCACCCGTTAAAT+TGG	+	chr5.3:5437631-5437650	None:intergenic	45.0%
	ATTTGAGCTGAGCTTGGGTC+GGG	+	chr5.3:5436892-5436911	None:intergenic	50.0%
	GATTTGAGCTGAGCTTGGGT+CGG	+	chr5.3:5436893-5436912	None:intergenic	50.0%
	GGAATCCTCATGCGTTCTCT+CGG	-	chr5.3:5436860-5436879	MS.gene025797:intron	50.0%
	GTTAGGGAAGACATGACACG+AGG	+	chr5.3:5437534-5437553	None:intergenic	50.0%
	TAGATTCTCTTCGGCGACGA+TGG	+	chr5.3:5436915-5436934	None:intergenic	50.0%
	TCGAGAGAAATCGAAAGGGG+AGG	+	chr5.3:5436939-5436958	None:intergenic	50.0%
!	CGATGGATTTGAGCTGAGCT+TGG	+	chr5.3:5436898-5436917	None:intergenic	50.0%
	AAGATCGCACCATCGGAGCT+CGG	-	chr5.3:5436839-5436858	MS.gene025797:intron	55.0%
	ATCCTCATGCGTTCTCTCGG+CGG	-	chr5.3:5436863-5436882	MS.gene025797:intron	55.0%
	ATGAGGATTCCGAGCTCCGA+TGG	+	chr5.3:5436851-5436870	None:intergenic	55.0%
	CTCTTCGACACCGACAACGA+CGG	-	chr5.3:5436815-5436834	MS.gene025797:intron	55.0%
	TGCGATCTTGCCGTCGTTGT+CGG	+	chr5.3:5436828-5436847	None:intergenic	55.0%
	CAGGGGTGGCACTCGAGAAT+CGG	-	chr5.3:5437921-5437940	MS.gene025797:CDS	60.0%
	CGACGGCAAGATCGCACCAT+CGG	-	chr5.3:5436832-5436851	MS.gene025797:intron	60.0%
	TACCGCCGAGAGAACGCATG+AGG	+	chr5.3:5436868-5436887	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	5436797	5438070	5436797	ID=MS.gene025797
chr5.3	mRNA	5436797	5438070	5436797	ID=MS.gene025797.t1;Parent=MS.gene025797
chr5.3	exon	5437651	5438070	5437651	ID=MS.gene025797.t1.exon1;Parent=MS.gene025797.t1
chr5.3	CDS	5437651	5438070	5437651	ID=cds.MS.gene025797.t1;Parent=MS.gene025797.t1
chr5.3	exon	5436797	5436829	5436797	ID=MS.gene025797.t1.exon2;Parent=MS.gene025797.t1
chr5.3	CDS	5436797	5436829	5436797	ID=cds.MS.gene025797.t1;Parent=MS.gene025797.t1

Gene Sequence

>MS.gene025797

ATGAAAGAAGCATTCACACTCTTCGACACCGACAACGACGGCAAGATCGCACCATCGGAGCTCGGAATCCTCATGCGTTCTCTCGGCGGTAACCCGACCCAAGCTCAGCTCAAATCCATCGTCGCCGAAGAGAATCTAACCTCCCCTTTCGATTTCTCTCGATTTCTCGATCTCATGTCCAAACACATGAAACCTGAACCGTTCGATCATCAACTAAGGGATGCGTTCAAAGTTCTCGATAAAGATGCAACTGGATTTGTTTCTGTGAATGAGCTTCGTCATATTTTGACGAGTATTGGTGAGAAGCTTGAGCCTGCTGAGTTTGATGAATGGATTAGGGAAGTTGATGTTGGGTTTGATGGGAAGATTCGTTATGAAGATTTCATCGCTAGAATGGTCGCCAAGTACATGCAATGCAATGTGGGAATGCTTTTTTATTTCTCTAAAATTATATTTTGTTTCTTTTTAACTGTCGTTTTTAGTTTATCTAAACATATACAAAGATCTAATAATATTTTTTTTGTTATTATTTGCCTTTTATTAAATATCATTAACTATATATTTTCTTATTTTTACCAATTTTTCTATCTCGAATAAATAGTTAAGGAGATTTTTGACAAAATAATAGTTGGTGTTGCATTCAATTATCGGAACGGTCCAAGGGTCAATTAGTCCAAGATAAGGTTTTAAGCTTTAAATTTTTGTTTCATGGCCACAAATAGTTAAGATGAATGCCTCGTGTCATGTCTTCCCTAACCTAGATTTTCCTTTTTTTTATTGACCAGTGCAATTTGTTTGTGTTTTATACTTGGTGAAATAGAAATTATGGGACCAATTTAACGGGTGCCCCTAATATGCACCCTTTTAAAAAGGGTCCAAGCATGGACCATTCATTTTTACCCACAGTAGCCGATTATCACTTTTTTTTTAATAATTGTTTTAAGTTCATTTTTTATTAAATACATCTCTCTCTCTCTCAACCACAATATTGTCCCGATTTTATCTCGATGCAGATTATTTTAGTATGAAAGATGAAAGTAGGAGTATTTGGTTATCATAAATATCAATTCTAAAATGGATTGTCAAATTTTTTGTTTACCGTCAAACTCAAAATTATTGAGTATCAGGGGTGGCACTCGAGAATCGGTGTTGTTTGAAACCAAAATGGAACATCTTGTTCCAAAAATAATCACAGACCAATGGTGTTATGTACATGGATAAAAGAACTGATTTTGTTTCAGATTTCTCAACAGAAACAGGACAAGTGGAAGTGA

Protein sequence

>MS.gene025797.t1

MKEAFTLFDTDNDGKIAPSELGILMRSLGGNPTQAQLKSIVAEENLTSPFDFSRFLDLMSKHMKPEPFDHQLRDAFKVLDKDATGFVSVNELRHILTSIGEKLEPAEFDEWIREVDVGFDGKIRYEDFIARMVAKYMQCNISQQKQDKWK