AlfalfaGEDB | MS.gene09960

M. sativa cultivar XinJiangDaYe / MS.gene09960

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF00036.35 PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09960.t1	XP_003611244.1	97.8	135	3	0	1	135	13	147	5.60E-68	266.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09960.t1	Q94AZ4	86.7	135	18	0	1	135	14	148	4.6e-63	241.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09960.t1	B7FNA2	97.8	135	3	0	1	135	13	147	4.0e-68	266.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene03228	MS.gene09960	PPI
MS.gene28338	MS.gene09960	PPI
MS.gene85596	MS.gene09960	PPI
MS.gene94551	MS.gene09960	PPI
MS.gene65954	MS.gene09960	PPI
MS.gene008225	MS.gene09960	PPI
MS.gene056383	MS.gene09960	PPI
MS.gene09960	MS.gene71511	PPI
MS.gene64324	MS.gene09960	PPI
MS.gene015470	MS.gene09960	PPI
MS.gene024717	MS.gene09960	PPI
MS.gene09960	MS.gene061768	PPI
MS.gene22553	MS.gene09960	PPI
MS.gene53662	MS.gene09960	PPI
MS.gene35563	MS.gene09960	PPI
MS.gene55496	MS.gene09960	PPI
MS.gene003880	MS.gene09960	PPI
MS.gene54637	MS.gene09960	PPI
MS.gene49271	MS.gene09960	PPI
MS.gene67281	MS.gene09960	PPI
MS.gene025777	MS.gene09960	PPI
MS.gene026940	MS.gene09960	PPI
MS.gene21155	MS.gene09960	PPI
MS.gene71207	MS.gene09960	PPI
MS.gene92775	MS.gene09960	PPI
MS.gene004899	MS.gene09960	PPI
MS.gene07422	MS.gene09960	PPI
MS.gene09934	MS.gene09960	PPI
MS.gene96922	MS.gene09960	PPI
MS.gene21932	MS.gene09960	PPI
MS.gene47134	MS.gene09960	PPI
MS.gene09960	MS.gene056461	PPI
MS.gene022455	MS.gene09960	PPI
MS.gene87673	MS.gene09960	PPI
MS.gene29793	MS.gene09960	PPI
MS.gene05284	MS.gene09960	PPI
MS.gene21931	MS.gene09960	PPI
MS.gene041795	MS.gene09960	PPI
MS.gene09960	MS.gene29459	PPI
MS.gene046988	MS.gene09960	PPI
MS.gene63000	MS.gene09960	PPI
MS.gene09960	MS.gene35562	PPI
MS.gene057293	MS.gene09960	PPI
MS.gene63281	MS.gene09960	PPI
MS.gene96914	MS.gene09960	PPI
MS.gene29794	MS.gene09960	PPI
MS.gene53648	MS.gene09960	PPI
MS.gene017446	MS.gene09960	PPI
MS.gene033361	MS.gene09960	PPI
MS.gene040127	MS.gene09960	PPI
MS.gene64310	MS.gene09960	PPI
MS.gene53659	MS.gene09960	PPI
MS.gene038456	MS.gene09960	PPI
MS.gene000724	MS.gene09960	PPI
MS.gene09960	MS.gene21696	PPI
MS.gene74452	MS.gene09960	PPI
MS.gene060446	MS.gene09960	PPI
MS.gene008831	MS.gene09960	PPI
MS.gene96913	MS.gene09960	PPI
MS.gene09960	MS.gene64317	PPI
MS.gene009021	MS.gene09960	PPI
MS.gene46237	MS.gene09960	PPI
MS.gene58860	MS.gene09960	PPI
MS.gene98294	MS.gene09960	PPI
MS.gene98295	MS.gene09960	PPI
MS.gene02545	MS.gene09960	PPI
MS.gene053319	MS.gene09960	PPI
MS.gene04915	MS.gene09960	PPI
MS.gene61009	MS.gene09960	PPI
MS.gene64327	MS.gene09960	PPI
MS.gene074100	MS.gene09960	PPI
MS.gene025797	MS.gene09960	PPI
MS.gene026618	MS.gene09960	PPI
MS.gene64316	MS.gene09960	PPI
MS.gene09960	MS.gene79028	PPI
MS.gene074045	MS.gene09960	PPI
MS.gene68345	MS.gene09960	PPI
MS.gene036930	MS.gene09960	PPI
MS.gene89216	MS.gene09960	PPI
MS.gene001246	MS.gene09960	PPI
MS.gene62258	MS.gene09960	PPI
MS.gene55545	MS.gene09960	PPI
MS.gene64311	MS.gene09960	PPI
MS.gene09960	MS.gene025797	PPI
MS.gene011400	MS.gene09960	PPI
MS.gene040331	MS.gene09960	PPI
MS.gene09960	MS.gene64314	PPI
MS.gene58906	MS.gene09960	PPI
MS.gene64308	MS.gene09960	PPI
MS.gene64313	MS.gene09960	PPI
MS.gene93087	MS.gene09960	PPI
MS.gene27516	MS.gene09960	PPI
MS.gene49270	MS.gene09960	PPI
MS.gene97948	MS.gene09960	PPI
MS.gene09960	MS.gene53650	PPI
MS.gene000738	MS.gene09960	PPI
MS.gene64307	MS.gene09960	PPI
MS.gene62540	MS.gene09960	PPI
MS.gene061261	MS.gene09960	PPI
MS.gene022456	MS.gene09960	PPI
MS.gene04917	MS.gene09960	PPI
MS.gene062239	MS.gene09960	PPI
MS.gene46886	MS.gene09960	PPI
MS.gene09960	MS.gene057167	PPI
MS.gene47195	MS.gene09960	PPI
MS.gene09960	MS.gene93763	PPI
MS.gene013748	MS.gene09960	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09960.t1	MTR_5g011850	97.778	135	3	0	1	135	13	147	8.70e-95	270
MS.gene09960.t1	MTR_5g088320	50.735	136	65	1	2	135	14	149	7.78e-44	141
MS.gene09960.t1	MTR_6g025320	50.735	136	65	1	2	135	14	149	1.06e-43	141
MS.gene09960.t1	MTR_7g087610	50.000	136	66	1	2	135	14	149	1.64e-43	140
MS.gene09960.t1	MTR_1g041285	42.647	136	76	1	1	134	13	148	4.91e-36	122
MS.gene09960.t1	MTR_1g076650	40.741	135	78	1	2	134	14	148	1.18e-34	118
MS.gene09960.t1	MTR_0168s0120	48.673	113	56	1	25	135	1	113	1.70e-33	114
MS.gene09960.t1	MTR_6g025320	48.673	113	56	1	25	135	1	113	1.70e-33	114
MS.gene09960.t1	MTR_1g071150	35.714	140	82	3	3	140	15	148	2.35e-26	97.4
MS.gene09960.t1	MTR_7g115040	37.313	134	82	1	3	134	15	148	1.78e-22	87.4
MS.gene09960.t1	MTR_8g066630	34.586	133	82	3	1	128	18	150	1.83e-21	85.1
MS.gene09960.t1	MTR_1g032070	33.824	136	88	1	1	134	47	182	9.65e-20	81.6
MS.gene09960.t1	MTR_8g105230	33.846	130	84	1	1	128	37	166	8.04e-19	79.0
MS.gene09960.t1	MTR_7g074020	31.343	134	90	1	1	132	31	164	1.81e-18	77.4
MS.gene09960.t1	MTR_6g007613	31.343	134	90	1	1	132	30	163	6.10e-18	76.3
MS.gene09960.t1	MTR_5g079340	33.824	136	85	3	1	133	5	138	4.82e-17	73.2
MS.gene09960.t1	MTR_4g107490	33.582	134	86	2	1	132	379	511	7.36e-17	76.6
MS.gene09960.t1	MTR_4g112460	31.618	136	89	2	1	132	12	147	1.22e-16	72.8
MS.gene09960.t1	MTR_7g074020	31.356	118	79	1	1	116	31	148	2.00e-16	72.0
MS.gene09960.t1	MTR_8g107110	33.588	131	82	2	1	128	25	153	3.22e-16	71.6
MS.gene09960.t1	MTR_6g007613	31.356	118	79	1	1	116	30	147	6.03e-16	70.9
MS.gene09960.t1	MTR_1g096490	30.597	134	88	3	1	132	336	466	2.66e-15	72.0
MS.gene09960.t1	MTR_3g055570	36.842	133	63	3	1	129	53	168	5.85e-15	68.6
MS.gene09960.t1	MTR_1g046950	30.657	137	91	2	1	133	5	141	6.29e-15	67.8
MS.gene09960.t1	MTR_6g079570	32.624	141	85	4	1	135	27	163	7.70e-15	68.2
MS.gene09960.t1	MTR_1g047100	29.197	137	94	2	1	134	5	141	1.54e-14	66.6
MS.gene09960.t1	MTR_1g055255	32.836	134	85	3	1	132	430	560	3.66e-14	68.9
MS.gene09960.t1	MTR_7g106710	29.851	134	89	3	1	132	441	571	4.68e-14	68.6
MS.gene09960.t1	MTR_3g051770	34.286	140	83	3	1	132	389	527	9.63e-14	67.8
MS.gene09960.t1	MTR_5g027000	27.737	137	95	2	3	137	25	159	9.83e-14	65.1
MS.gene09960.t1	MTR_3g067610	29.231	130	86	2	1	128	6	131	2.94e-13	63.2
MS.gene09960.t1	MTR_7g068710	31.343	134	89	2	1	132	362	494	3.30e-13	66.2
MS.gene09960.t1	MTR_4g067270	30.882	136	90	2	1	132	6	141	4.96e-13	62.8
MS.gene09960.t1	MTR_1g041150	30.597	134	88	3	1	132	424	554	6.47e-13	65.5
MS.gene09960.t1	MTR_7g075040	31.690	142	86	3	2	132	49	190	1.95e-12	62.4
MS.gene09960.t1	MTR_1g054865	31.343	134	87	3	1	132	467	597	2.11e-12	63.9
MS.gene09960.t1	MTR_8g069915	25.161	155	109	3	1	148	13	167	3.18e-12	61.2
MS.gene09960.t1	MTR_8g095440	28.358	134	91	3	1	132	336	466	3.63e-12	63.2
MS.gene09960.t1	MTR_3g098070	27.612	134	92	3	1	132	335	465	4.22e-12	62.8
MS.gene09960.t1	MTR_3g098090	27.612	134	92	3	1	132	338	468	5.28e-12	62.8
MS.gene09960.t1	MTR_8g036075	31.915	141	86	3	2	132	51	191	6.56e-12	60.8
MS.gene09960.t1	MTR_1g052530	29.412	136	93	2	1	134	358	492	8.99e-12	62.0
MS.gene09960.t1	MTR_5g092810	31.429	140	92	3	1	138	398	535	1.09e-11	61.6
MS.gene09960.t1	MTR_3g083470	31.250	128	80	2	1	128	94	213	1.18e-11	60.5
MS.gene09960.t1	MTR_5g099240	28.358	134	91	3	1	132	402	532	1.89e-11	61.2
MS.gene09960.t1	MTR_7g091890	30.147	136	92	2	1	134	364	498	2.04e-11	60.8
MS.gene09960.t1	MTR_5g099240	28.358	134	91	3	1	132	330	460	2.08e-11	60.8
MS.gene09960.t1	MTR_4g103650	31.538	130	80	4	4	128	6	131	5.55e-11	57.0
MS.gene09960.t1	MTR_4g103655	31.538	130	80	4	4	128	10	135	5.57e-11	57.4
MS.gene09960.t1	MTR_4g132070	32.090	134	87	3	1	132	397	528	5.78e-11	59.7
MS.gene09960.t1	MTR_5g008705	26.923	130	93	1	7	134	1	130	7.19e-11	56.6
MS.gene09960.t1	MTR_4g115170	30.405	148	83	3	1	128	65	212	8.99e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09960.t1	AT1G12310	86.667	135	18	0	1	135	14	148	1.65e-84	244
MS.gene09960.t1	AT1G62820	83.704	135	22	0	1	135	14	148	7.25e-83	240
MS.gene09960.t1	AT5G21274	50.735	136	65	1	2	135	14	149	7.64e-44	141
MS.gene09960.t1	AT3G43810	50.735	136	65	1	2	135	14	149	1.11e-43	141
MS.gene09960.t1	AT5G37780	50.735	136	65	1	2	135	14	149	2.30e-43	140
MS.gene09960.t1	AT1G66410	50.735	136	65	1	2	135	14	149	2.30e-43	140
MS.gene09960.t1	AT3G56800	50.000	136	66	1	2	135	14	149	3.33e-43	140
MS.gene09960.t1	AT2G27030	50.000	136	66	1	2	135	14	149	3.33e-43	140
MS.gene09960.t1	AT2G41110	50.000	136	66	1	2	135	14	149	3.33e-43	140
MS.gene09960.t1	AT2G27030	50.000	136	66	1	2	135	14	149	6.30e-43	140
MS.gene09960.t1	AT3G43810	49.275	138	68	1	2	137	14	151	1.88e-42	140
MS.gene09960.t1	AT2G41110	45.946	148	66	2	2	135	14	161	1.42e-39	131
MS.gene09960.t1	AT5G37780	45.695	151	65	2	2	135	14	164	4.18e-39	130
MS.gene09960.t1	AT5G37780	42.593	162	65	2	2	135	14	175	4.21e-37	125
MS.gene09960.t1	AT1G66410	47.260	146	65	2	2	135	14	159	6.23e-37	124
MS.gene09960.t1	AT5G37780	48.387	124	62	1	14	135	13	136	2.24e-36	122
MS.gene09960.t1	AT3G22930	41.791	134	76	1	2	133	37	170	8.81e-36	122
MS.gene09960.t1	AT4G14640	40.299	134	78	1	2	133	15	148	1.74e-34	118
MS.gene09960.t1	AT3G43810	48.673	113	56	1	25	135	1	113	1.79e-33	114
MS.gene09960.t1	AT3G43810	48.673	113	56	1	25	135	1	113	1.79e-33	114
MS.gene09960.t1	AT1G66410	48.673	113	56	1	25	135	1	113	4.09e-33	113
MS.gene09960.t1	AT1G66410	48.673	113	56	1	25	135	1	113	4.09e-33	113
MS.gene09960.t1	AT4G14640	36.885	122	75	1	14	133	9	130	1.43e-27	100
MS.gene09960.t1	AT2G41100	35.714	168	86	4	2	150	68	232	5.94e-25	97.1
MS.gene09960.t1	AT2G41100	29.688	128	72	3	24	133	1	128	2.56e-11	60.5
MS.gene09960.t1	AT2G41100	35.714	168	86	4	2	150	68	232	5.94e-25	97.1
MS.gene09960.t1	AT2G41100	29.688	128	72	3	24	133	1	128	2.56e-11	60.5
MS.gene09960.t1	AT2G41100	35.714	168	86	4	2	150	68	232	5.94e-25	97.1
MS.gene09960.t1	AT2G41100	29.688	128	72	3	24	133	1	128	2.56e-11	60.5
MS.gene09960.t1	AT2G41100	35.714	168	86	4	2	150	103	267	9.63e-25	97.1
MS.gene09960.t1	AT2G41100	32.667	150	83	3	2	133	14	163	2.36e-19	82.8
MS.gene09960.t1	AT2G41100	35.714	168	86	4	2	150	103	267	9.63e-25	97.1
MS.gene09960.t1	AT2G41100	32.667	150	83	3	2	133	14	163	2.36e-19	82.8
MS.gene09960.t1	AT2G41090	36.765	136	81	2	2	135	14	146	8.56e-22	86.7
MS.gene09960.t1	AT4G14640	42.553	94	52	1	2	93	15	108	2.01e-21	84.0
MS.gene09960.t1	AT3G22930	41.935	93	52	1	2	92	37	129	1.05e-20	82.4
MS.gene09960.t1	AT3G50360	31.343	134	90	1	1	132	28	161	1.40e-19	80.5
MS.gene09960.t1	AT4G37010	32.836	134	88	1	1	132	28	161	8.06e-19	78.6
MS.gene09960.t1	AT4G37010	32.836	134	88	1	1	132	32	165	8.08e-19	78.6
MS.gene09960.t1	AT2G41100	34.483	145	73	4	25	150	1	142	2.75e-18	77.8
MS.gene09960.t1	AT3G51920	31.343	134	90	1	3	134	15	148	5.58e-18	75.9
MS.gene09960.t1	AT1G24620	32.867	143	94	1	1	141	38	180	6.28e-18	76.6
MS.gene09960.t1	AT1G32250	30.882	136	86	3	1	128	17	152	1.83e-17	74.7
MS.gene09960.t1	AT1G74740	35.766	137	83	4	1	134	365	499	3.11e-16	74.7
MS.gene09960.t1	AT1G18530	32.374	139	89	3	1	135	8	145	3.76e-15	68.6
MS.gene09960.t1	AT1G35670	32.090	134	86	3	1	132	332	462	5.01e-15	71.2
MS.gene09960.t1	AT1G76040	33.582	134	85	3	1	132	179	310	5.31e-15	70.9
MS.gene09960.t1	AT1G76040	33.582	134	85	3	1	132	179	310	5.31e-15	70.9
MS.gene09960.t1	AT3G03000	32.847	137	89	2	1	134	21	157	6.22e-15	68.2
MS.gene09960.t1	AT1G76040	33.582	134	85	3	1	132	417	548	6.26e-15	71.2
MS.gene09960.t1	AT4G09570	31.343	134	87	3	1	132	331	461	2.40e-14	69.3
MS.gene09960.t1	AT1G18210	29.323	133	92	1	1	133	24	154	3.27e-14	66.6
MS.gene09960.t1	AT1G18210	29.323	133	92	1	1	133	24	154	3.27e-14	66.6
MS.gene09960.t1	AT1G66400	30.657	137	91	2	1	133	16	152	4.04e-14	65.9
MS.gene09960.t1	AT5G23580	29.851	134	89	3	1	132	328	458	3.67e-13	65.9
MS.gene09960.t1	AT4G38230	29.104	134	90	3	1	132	360	490	7.57e-13	65.1
MS.gene09960.t1	AT4G38230	29.104	134	90	3	1	132	330	460	7.69e-13	65.1
MS.gene09960.t1	AT4G38230	29.104	134	90	3	1	132	330	460	7.69e-13	65.1
MS.gene09960.t1	AT4G38230	28.378	148	97	4	1	142	186	330	9.07e-13	64.7
MS.gene09960.t1	AT3G10660	31.343	134	87	3	1	132	492	622	1.15e-12	64.7
MS.gene09960.t1	AT3G10660	31.343	134	87	3	1	132	492	622	1.15e-12	64.7
MS.gene09960.t1	AT1G05990	30.827	133	87	3	1	128	6	138	1.26e-12	62.0
MS.gene09960.t1	AT3G59440	31.884	138	85	4	1	132	52	186	1.59e-12	62.4
MS.gene09960.t1	AT2G43290	29.577	142	89	4	1	132	66	206	3.08e-12	62.0
MS.gene09960.t1	AT3G20410	31.343	134	88	3	1	132	397	528	5.51e-12	62.8
MS.gene09960.t1	AT3G20410	31.343	134	88	3	1	132	397	528	5.51e-12	62.8
MS.gene09960.t1	AT2G17290	28.358	134	91	3	1	132	391	521	5.63e-12	62.8
MS.gene09960.t1	AT2G17290	28.358	134	91	3	1	132	391	521	5.63e-12	62.8
MS.gene09960.t1	AT2G15680	28.358	134	92	2	1	132	51	182	6.43e-12	60.8
MS.gene09960.t1	AT5G04870	29.851	134	89	3	1	132	456	586	7.52e-12	62.4
MS.gene09960.t1	AT2G38910	29.851	134	89	3	1	132	440	570	7.61e-12	62.4
MS.gene09960.t1	AT2G41860	30.597	134	90	2	1	132	360	492	8.07e-12	62.0
MS.gene09960.t1	AT4G03290	30.370	135	87	3	1	128	6	140	8.30e-12	59.7
MS.gene09960.t1	AT2G41860	30.597	134	90	2	1	132	360	492	8.47e-12	62.0
MS.gene09960.t1	AT1G73630	26.923	130	89	2	1	128	21	146	9.27e-12	59.7
MS.gene09960.t1	AT4G21940	32.090	134	87	3	1	132	407	538	1.14e-11	61.6
MS.gene09960.t1	AT4G21940	32.090	134	87	3	1	132	407	538	1.24e-11	61.6
MS.gene09960.t1	AT4G35310	29.104	134	90	3	1	132	403	533	2.64e-11	60.8
MS.gene09960.t1	AT4G35310	29.104	134	90	3	1	132	403	533	2.64e-11	60.8
MS.gene09960.t1	AT3G57530	30.935	139	82	4	1	132	369	500	3.24e-11	60.5
MS.gene09960.t1	AT1G18890	29.927	137	91	4	1	134	369	503	3.61e-11	60.5
MS.gene09960.t1	AT2G41100	32.500	80	51	1	71	150	12	88	3.88e-11	57.8
MS.gene09960.t1	AT4G23650	30.597	134	89	3	1	132	384	515	5.87e-11	59.7
MS.gene09960.t1	AT3G25600	26.846	149	102	3	1	142	13	161	6.65e-11	57.4
MS.gene09960.t1	AT5G12480	29.286	140	89	4	1	134	365	500	8.15e-11	59.3

Find 36 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTTCAGGTTTCATGTGTT+TGG	0.324663	5.2:+4642248	None:intergenic
CACATTGCATTGCATGTACT+TGG	0.334219	5.2:+4642026	None:intergenic
CTGAGTTTGATGAATGGATT+AGG	0.334899	5.2:-4642110	MS.gene09960:CDS
AGTTGATGATCGAACGGTTC+AGG	0.354968	5.2:+4642234	None:intergenic
TCAGATTTCTCAACAGAAAC+AGG	0.371452	5.2:-4641188	MS.gene09960:intron
GAAGTTGATGTTGGGTTTGA+TGG	0.388552	5.2:-4642087	MS.gene09960:CDS
CGATGGATTTGAGCTGAGCT+TGG	0.412141	5.2:+4642328	None:intergenic
TGCGATCTTGCCGTCGTTGT+CGG	0.415377	5.2:+4642398	None:intergenic
GGATTAGGGAAGTTGATGTT+GGG	0.442492	5.2:-4642095	MS.gene09960:CDS
TGGATTAGGGAAGTTGATGT+TGG	0.452504	5.2:-4642096	MS.gene09960:CDS
GTTCTCGATAAAGATGCAAC+TGG	0.460205	5.2:-4642195	MS.gene09960:CDS
ATTTGAGCTGAGCTTGGGTC+GGG	0.477294	5.2:+4642334	None:intergenic
AGCCTGCTGAGTTTGATGAA+TGG	0.478011	5.2:-4642116	MS.gene09960:CDS
TGAGTTTGATGAATGGATTA+GGG	0.481850	5.2:-4642109	MS.gene09960:CDS
AACCGTTCGATCATCAACTA+AGG	0.488078	5.2:-4642230	MS.gene09960:CDS
GATGGATTTGAGCTGAGCTT+GGG	0.498752	5.2:+4642329	None:intergenic
TGAAGATTTCATCGCTAGAA+TGG	0.506739	5.2:-4642052	MS.gene09960:CDS
AGGGGAGGTTAGATTCTCTT+CGG	0.513129	5.2:+4642302	None:intergenic
ATCCATTCATCAAACTCAGC+AGG	0.514616	5.2:+4642114	None:intergenic
TAGATTCTCTTCGGCGACGA+TGG	0.518627	5.2:+4642311	None:intergenic
GATTTGAGCTGAGCTTGGGT+CGG	0.523421	5.2:+4642333	None:intergenic
GGAATCCTCATGCGTTCTCT+CGG	0.535690	5.2:-4642363	MS.gene09960:CDS
AAGTTGATGTTGGGTTTGAT+GGG	0.546897	5.2:-4642086	MS.gene09960:CDS
CGACGGCAAGATCGCACCAT+CGG	0.559292	5.2:-4642391	MS.gene09960:CDS
CTCAACAGAAACAGGACAAG+TGG	0.561226	5.2:-4641180	MS.gene09960:CDS
GAAATCGAGAGAAATCGAAA+GGG	0.564111	5.2:+4642283	None:intergenic
AAGATCGCACCATCGGAGCT+CGG	0.573545	5.2:-4642384	MS.gene09960:CDS
AGAAATCGAGAGAAATCGAA+AGG	0.584540	5.2:+4642282	None:intergenic
TCCCTTAGTTGATGATCGAA+CGG	0.595563	5.2:+4642228	None:intergenic
ATGAGGATTCCGAGCTCCGA+TGG	0.601818	5.2:+4642375	None:intergenic
ATCCTCATGCGTTCTCTCGG+CGG	0.614038	5.2:-4642360	MS.gene09960:CDS
ACCGTTCGATCATCAACTAA+GGG	0.620555	5.2:-4642229	MS.gene09960:CDS
TACCGCCGAGAGAACGCATG+AGG	0.654227	5.2:+4642358	None:intergenic
CTCTTCGACACCGACAACGA+CGG	0.661849	5.2:-4642408	MS.gene09960:CDS
TCGAGAGAAATCGAAAGGGG+AGG	0.668937	5.2:+4642287	None:intergenic
AAATCGAGAGAAATCGAAAG+GGG	0.678072	5.2:+4642284	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTTAATGATATTTAATAAA+AGG	+	chr5.2:4641711-4641730	None:intergenic	10.0%
!!	ATTAAAAAAAAAGTGATAAT+CGG	+	chr5.2:4642087-4642106	None:intergenic	10.0%
!!	AATAAAAAAAAGGAAAATCT+AGG	+	chr5.2:4641934-4641953	None:intergenic	15.0%
!!!	ATTTTTGACAAAATAATAGT+TGG	-	chr5.2:4641784-4641803	MS.gene09960:intron	15.0%
!!!	CATAAATATCAATTCTAAAA+TGG	-	chr5.2:4642230-4642249	MS.gene09960:CDS	15.0%
!!	TTTATTCGAGATAGAAAAAT+TGG	+	chr5.2:4641753-4641772	None:intergenic	20.0%
!!!	AGCTTTAAATTTTTGTTTCA+TGG	-	chr5.2:4641864-4641883	MS.gene09960:intron	20.0%
!!!	ATTTGTTTGTGTTTTATACT+TGG	-	chr5.2:4641964-4641983	MS.gene09960:intron	20.0%
!	AAAAAAGGAAAATCTAGGTT+AGG	+	chr5.2:4641929-4641948	None:intergenic	25.0%
!	AAAAAGGAAAATCTAGGTTA+GGG	+	chr5.2:4641928-4641947	None:intergenic	25.0%
!	AAATTATGGGACCAATTTAA+CGG	-	chr5.2:4641995-4642014	MS.gene09960:intron	25.0%
!	ACTCAAAATTATTGAGTATC+AGG	-	chr5.2:4642280-4642299	MS.gene09960:CDS	25.0%
!	CTATCTCGAATAAATAGTTA+AGG	-	chr5.2:4641759-4641778	MS.gene09960:intron	25.0%
!	CTCAAAATTATTGAGTATCA+GGG	-	chr5.2:4642281-4642300	MS.gene09960:CDS	25.0%
!	CTTGGTGAAATAGAAATTAT+GGG	-	chr5.2:4641982-4642001	MS.gene09960:intron	25.0%
!	TAAAGCTTAAAACCTTATCT+TGG	+	chr5.2:4641851-4641870	None:intergenic	25.0%
!	TCAAAATTATTGAGTATCAG+GGG	-	chr5.2:4642282-4642301	MS.gene09960:CDS	25.0%
!	TTAGTATGAAAGATGAAAGT+AGG	-	chr5.2:4642194-4642213	MS.gene09960:CDS	25.0%
!!	ACTTGGTGAAATAGAAATTA+TGG	-	chr5.2:4641981-4642000	MS.gene09960:intron	25.0%
!!!	AATATGCACCCTTTTAAAAA+GGG	-	chr5.2:4642026-4642045	MS.gene09960:intron	25.0%
!!!	CTCAATAATTTTGAGTTTGA+CGG	+	chr5.2:4642276-4642295	None:intergenic	25.0%
!!!	TAATATGCACCCTTTTAAAA+AGG	-	chr5.2:4642025-4642044	MS.gene09960:intron	25.0%
!!!	TTTTAAAAGGGTGCATATTA+GGG	+	chr5.2:4642025-4642044	None:intergenic	25.0%
!!!	TTTTTAAAAGGGTGCATATT+AGG	+	chr5.2:4642026-4642045	None:intergenic	25.0%
	AAAACACAAACAAATTGCAC+TGG	+	chr5.2:4641959-4641978	None:intergenic	30.0%
	AATCTGCATCGAGATAAAAT+CGG	+	chr5.2:4642171-4642190	None:intergenic	30.0%
	AATTATGGGACCAATTTAAC+GGG	-	chr5.2:4641996-4642015	MS.gene09960:intron	30.0%
	ATAAAATCGGGACAATATTG+TGG	+	chr5.2:4642158-4642177	None:intergenic	30.0%
	CAAAAATAATCACAGACCAA+TGG	-	chr5.2:4642355-4642374	MS.gene09960:CDS	30.0%
	GCATTCATCTTAACTATTTG+TGG	+	chr5.2:4641890-4641909	None:intergenic	30.0%
!	TGCACTGGTCAATAAAAAAA+AGG	+	chr5.2:4641944-4641963	None:intergenic	30.0%
!	TTTAAAAGGGTGCATATTAG+GGG	+	chr5.2:4642024-4642043	None:intergenic	30.0%
!!	AAGATGAAAGTAGGAGTATT+TGG	-	chr5.2:4642203-4642222	MS.gene09960:CDS	30.0%
!!	CATTGGTCTGTGATTATTTT+TGG	+	chr5.2:4642357-4642376	None:intergenic	30.0%
!!	TGAGTTTGATGAATGGATTA+GGG	-	chr5.2:4641491-4641510	MS.gene09960:intron	30.0%
!!	TTGGTGTTGCATTCAATTAT+CGG	-	chr5.2:4641803-4641822	MS.gene09960:intron	30.0%
	AAAAATGAATGGTCCATGCT+TGG	+	chr5.2:4642053-4642072	None:intergenic	35.0%
	AAATCGAGAGAAATCGAAAG+GGG	+	chr5.2:4641319-4641338	None:intergenic	35.0%
	AAATTATTGAGTATCAGGGG+TGG	-	chr5.2:4642285-4642304	MS.gene09960:CDS	35.0%
	AAGTACATGCAATGCAATGT+GGG	-	chr5.2:4641576-4641595	MS.gene09960:intron	35.0%
	AGAAATCGAGAGAAATCGAA+AGG	+	chr5.2:4641321-4641340	None:intergenic	35.0%
	ATCCATGTACATAACACCAT+TGG	+	chr5.2:4642374-4642393	None:intergenic	35.0%
	ATCTGCATCGAGATAAAATC+GGG	+	chr5.2:4642170-4642189	None:intergenic	35.0%
	CTACTGTGGGTAAAAATGAA+TGG	+	chr5.2:4642064-4642083	None:intergenic	35.0%
	GAAATCGAGAGAAATCGAAA+GGG	+	chr5.2:4641320-4641339	None:intergenic	35.0%
	GTTGCATTCAATTATCGGAA+CGG	-	chr5.2:4641808-4641827	MS.gene09960:intron	35.0%
	TCAGATTTCTCAACAGAAAC+AGG	-	chr5.2:4642412-4642431	MS.gene09960:CDS	35.0%
	TGAAGATTTCATCGCTAGAA+TGG	-	chr5.2:4641548-4641567	MS.gene09960:intron	35.0%
!	ATGCTTGGACCCTTTTTAAA+AGG	+	chr5.2:4642038-4642057	None:intergenic	35.0%
!	CTGAGTTTGATGAATGGATT+AGG	-	chr5.2:4641490-4641509	MS.gene09960:intron	35.0%
!	TGGAACAAGATGTTCCATTT+TGG	+	chr5.2:4642337-4642356	None:intergenic	35.0%
!!	AAGTTGATGTTGGGTTTGAT+GGG	-	chr5.2:4641514-4641533	MS.gene09960:intron	35.0%
!!	GGTGTTGTTTGAAACCAAAA+TGG	-	chr5.2:4642320-4642339	MS.gene09960:CDS	35.0%
!!	TGCTTGGACCCTTTTTAAAA+GGG	+	chr5.2:4642037-4642056	None:intergenic	35.0%
!!	TTTTAAAAAGGGTCCAAGCA+TGG	-	chr5.2:4642037-4642056	MS.gene09960:CDS	35.0%
!!!	CGTCATATTTTGACGAGTAT+TGG	-	chr5.2:4641450-4641469	MS.gene09960:intron	35.0%
	AAAGTGATAATCGGCTACTG+TGG	+	chr5.2:4642078-4642097	None:intergenic	40.0%
	AACCGTTCGATCATCAACTA+AGG	-	chr5.2:4641370-4641389	MS.gene09960:intron	40.0%
	AAGTGATAATCGGCTACTGT+GGG	+	chr5.2:4642077-4642096	None:intergenic	40.0%
	ACCGTTCGATCATCAACTAA+GGG	-	chr5.2:4641371-4641390	MS.gene09960:intron	40.0%
	ATCCATTCATCAAACTCAGC+AGG	+	chr5.2:4641489-4641508	None:intergenic	40.0%
	ATCTTGGACTAATTGACCCT+TGG	+	chr5.2:4641835-4641854	None:intergenic	40.0%
	CAAGTACATGCAATGCAATG+TGG	-	chr5.2:4641575-4641594	MS.gene09960:intron	40.0%
	CACATTGCATTGCATGTACT+TGG	+	chr5.2:4641577-4641596	None:intergenic	40.0%
	GGGTCAATTAGTCCAAGATA+AGG	-	chr5.2:4641836-4641855	MS.gene09960:intron	40.0%
	GTTCTCGATAAAGATGCAAC+TGG	-	chr5.2:4641405-4641424	MS.gene09960:intron	40.0%
!!	GAAGTTGATGTTGGGTTTGA+TGG	-	chr5.2:4641513-4641532	MS.gene09960:intron	40.0%
!!	GACCAATGGTGTTATGTACA+TGG	-	chr5.2:4642369-4642388	MS.gene09960:CDS	40.0%
!!	GGATTAGGGAAGTTGATGTT+GGG	-	chr5.2:4641505-4641524	MS.gene09960:intron	40.0%
!!	TCCCTTAGTTGATGATCGAA+CGG	+	chr5.2:4641375-4641394	None:intergenic	40.0%
!!	TGGATTAGGGAAGTTGATGT+TGG	-	chr5.2:4641504-4641523	MS.gene09960:intron	40.0%
	AGCCTGCTGAGTTTGATGAA+TGG	-	chr5.2:4641484-4641503	MS.gene09960:intron	45.0%
	AGGGGAGGTTAGATTCTCTT+CGG	+	chr5.2:4641301-4641320	None:intergenic	45.0%
	CAATTATCGGAACGGTCCAA+GGG	-	chr5.2:4641816-4641835	MS.gene09960:intron	45.0%
	CTCAACAGAAACAGGACAAG+TGG	-	chr5.2:4642420-4642439	MS.gene09960:CDS	45.0%
	TCAATTATCGGAACGGTCCA+AGG	-	chr5.2:4641815-4641834	MS.gene09960:intron	45.0%
!	CGGTTCAGGTTTCATGTGTT+TGG	+	chr5.2:4641355-4641374	None:intergenic	45.0%
!	GATGGATTTGAGCTGAGCTT+GGG	+	chr5.2:4641274-4641293	None:intergenic	45.0%
!!	AGTTGATGATCGAACGGTTC+AGG	+	chr5.2:4641369-4641388	None:intergenic	45.0%
!!	ATTAGGGGCACCCGTTAAAT+TGG	+	chr5.2:4642009-4642028	None:intergenic	45.0%
	ATTTGAGCTGAGCTTGGGTC+GGG	+	chr5.2:4641269-4641288	None:intergenic	50.0%
	GATTTGAGCTGAGCTTGGGT+CGG	+	chr5.2:4641270-4641289	None:intergenic	50.0%
	GGAATCCTCATGCGTTCTCT+CGG	-	chr5.2:4641237-4641256	MS.gene09960:intron	50.0%
	GTTAGGGAAGACATGACACG+AGG	+	chr5.2:4641912-4641931	None:intergenic	50.0%
	TAGATTCTCTTCGGCGACGA+TGG	+	chr5.2:4641292-4641311	None:intergenic	50.0%
	TCGAGAGAAATCGAAAGGGG+AGG	+	chr5.2:4641316-4641335	None:intergenic	50.0%
!	CGATGGATTTGAGCTGAGCT+TGG	+	chr5.2:4641275-4641294	None:intergenic	50.0%
	AAGATCGCACCATCGGAGCT+CGG	-	chr5.2:4641216-4641235	MS.gene09960:intron	55.0%
	ATCCTCATGCGTTCTCTCGG+CGG	-	chr5.2:4641240-4641259	MS.gene09960:intron	55.0%
	ATGAGGATTCCGAGCTCCGA+TGG	+	chr5.2:4641228-4641247	None:intergenic	55.0%
	CTCTTCGACACCGACAACGA+CGG	-	chr5.2:4641192-4641211	MS.gene09960:intron	55.0%
	TGCGATCTTGCCGTCGTTGT+CGG	+	chr5.2:4641205-4641224	None:intergenic	55.0%
	CAGGGGTGGCACTCGAGAAT+CGG	-	chr5.2:4642299-4642318	MS.gene09960:CDS	60.0%
	CGACGGCAAGATCGCACCAT+CGG	-	chr5.2:4641209-4641228	MS.gene09960:intron	60.0%
	TACCGCCGAGAGAACGCATG+AGG	+	chr5.2:4641245-4641264	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	4641174	4642448	4641174	ID=MS.gene09960
chr5.2	mRNA	4641174	4642448	4641174	ID=MS.gene09960.t1;Parent=MS.gene09960
chr5.2	exon	4642029	4642448	4642029	ID=MS.gene09960.t1.exon1;Parent=MS.gene09960.t1
chr5.2	CDS	4642029	4642448	4642029	ID=cds.MS.gene09960.t1;Parent=MS.gene09960.t1
chr5.2	exon	4641174	4641206	4641174	ID=MS.gene09960.t1.exon2;Parent=MS.gene09960.t1
chr5.2	CDS	4641174	4641206	4641174	ID=cds.MS.gene09960.t1;Parent=MS.gene09960.t1

Gene Sequence

>MS.gene09960

ATGAAAGAAGCATTCACACTCTTCGACACCGACAACGACGGCAAGATCGCACCATCGGAGCTCGGAATCCTCATGCGTTCTCTCGGCGGTAACCCGACCCAAGCTCAGCTCAAATCCATCGTCGCCGAAGAGAATCTAACCTCCCCTTTCGATTTCTCTCGATTTCTCGATCTCATGTCCAAACACATGAAACCTGAACCGTTCGATCATCAACTAAGGGATGCGTTCAAAGTTCTCGATAAAGATGCAACTGGATTTGTTTCTGTGAATGAGCTTCGTCATATTTTGACGAGTATTGGTGAGAAGCTTGAGCCTGCTGAGTTTGATGAATGGATTAGGGAAGTTGATGTTGGGTTTGATGGGAAGATTCGTTATGAAGATTTCATCGCTAGAATGGTCGCCAAGTACATGCAATGCAATGTGGGAATGCTTTTTTATTTCTCTAAAATTATATTTTGTTTCTTTTTAACTGTCGTTTTTAGTTTATCTAAACATATACAAAGATCTAATAATATTTTTTTTTGTTATTATTTGCCTTTTATTAAATATCATTAACTATATATTTTCTTATTTTTACCAATTTTTCTATCTCGAATAAATAGTTAAGGAGATTTTTGACAAAATAATAGTTGGTGTTGCATTCAATTATCGGAACGGTCCAAGGGTCAATTAGTCCAAGATAAGGTTTTAAGCTTTAAATTTTTGTTTCATGGCCACAAATAGTTAAGATGAATGCCTCGTGTCATGTCTTCCCTAACCTAGATTTTCCTTTTTTTTATTGACCAGTGCAATTTGTTTGTGTTTTATACTTGGTGAAATAGAAATTATGGGACCAATTTAACGGGTGCCCCTAATATGCACCCTTTTAAAAAGGGTCCAAGCATGGACCATTCATTTTTACCCACAGTAGCCGATTATCACTTTTTTTTTAATAATTGTTTTAAGTTCATTTTTTATTAAATACATCTCTCTCTCTCTCAACCACAATATTGTCCCGATTTTATCTCGATGCAGATTATTTTAGTATGAAAGATGAAAGTAGGAGTATTTGGTTATCATAAATATCAATTCTAAAATGGATTGTCAAATTTTTTGTTTACCGTCAAACTCAAAATTATTGAGTATCAGGGGTGGCACTCGAGAATCGGTGTTGTTTGAAACCAAAATGGAACATCTTGTTCCAAAAATAATCACAGACCAATGGTGTTATGTACATGGATAAAAGAACTGATTTTGTTTCAGATTTCTCAACAGAAACAGGACAAGTGGAAGTGA

Protein sequence

>MS.gene09960.t1

MKEAFTLFDTDNDGKIAPSELGILMRSLGGNPTQAQLKSIVAEENLTSPFDFSRFLDLMSKHMKPEPFDHQLRDAFKVLDKDATGFVSVNELRHILTSIGEKLEPAEFDEWIREVDVGFDGKIRYEDFIARMVAKYMQCNISQQKQDKWK