AlfalfaGEDB | MS.gene03228

M. sativa cultivar XinJiangDaYe / MS.gene03228

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13202.9 PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03228.t1	KEH35357.1	97.2	142	4	0	1	142	1	142	2.10E-69	271.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03228.t1	Q9LPK5	39.4	142	80	3	2	142	4	140	2.3e-19	96.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03228.t1	A0A072V0Q8	97.2	142	4	0	1	142	1	142	1.5e-69	271.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene03228	MS.gene09960	PPI
MS.gene074100	MS.gene03228	PPI
MS.gene03228	MS.gene53650	PPI
MS.gene63281	MS.gene03228	PPI
MS.gene68345	MS.gene03228	PPI
MS.gene066863	MS.gene03228	PPI
MS.gene03228	MS.gene057167	PPI
MS.gene001246	MS.gene03228	PPI
MS.gene03228	MS.gene93763	PPI
MS.gene040127	MS.gene03228	PPI
MS.gene62258	MS.gene03228	PPI
MS.gene03228	MS.gene80012	PPI
MS.gene55545	MS.gene03228	PPI
MS.gene000724	MS.gene03228	PPI
MS.gene052672	MS.gene03228	PPI
MS.gene74452	MS.gene03228	PPI
MS.gene64311	MS.gene03228	PPI
MS.gene03228	MS.gene29794	PPI
MS.gene040331	MS.gene03228	PPI
MS.gene03228	MS.gene71511	PPI
MS.gene58906	MS.gene03228	PPI
MS.gene03228	MS.gene033361	PPI
MS.gene020515	MS.gene03228	PPI
MS.gene03228	MS.gene98972	PPI
MS.gene036915	MS.gene03228	PPI
MS.gene58860	MS.gene03228	PPI
MS.gene64313	MS.gene03228	PPI
MS.gene83068	MS.gene03228	PPI
MS.gene03228	MS.gene061768	PPI
MS.gene03228	MS.gene89216	PPI
MS.gene27516	MS.gene03228	PPI
MS.gene053319	MS.gene03228	PPI
MS.gene04915	MS.gene03228	PPI
MS.gene49270	MS.gene03228	PPI
MS.gene97948	MS.gene03228	PPI
MS.gene61009	MS.gene03228	PPI
MS.gene64327	MS.gene03228	PPI
MS.gene000738	MS.gene03228	PPI
MS.gene64307	MS.gene03228	PPI
MS.gene061261	MS.gene03228	PPI
MS.gene04917	MS.gene03228	PPI
MS.gene03228	MS.gene07422	PPI
MS.gene026530	MS.gene03228	PPI
MS.gene47195	MS.gene03228	PPI
MS.gene92789	MS.gene03228	PPI
MS.gene036930	MS.gene03228	PPI
MS.gene03228	MS.gene64316	PPI
MS.gene03228	MS.gene98294	PPI
MS.gene28338	MS.gene03228	PPI
MS.gene008225	MS.gene03228	PPI
MS.gene64324	MS.gene03228	PPI
MS.gene011400	MS.gene03228	PPI
MS.gene015470	MS.gene03228	PPI
MS.gene066862	MS.gene03228	PPI
MS.gene03228	MS.gene056461	PPI
MS.gene015658	MS.gene03228	PPI
MS.gene024717	MS.gene03228	PPI
MS.gene03228	MS.gene64310	PPI
MS.gene64308	MS.gene03228	PPI
MS.gene03228	MS.gene29459	PPI
MS.gene53662	MS.gene03228	PPI
MS.gene03228	MS.gene71207	PPI
MS.gene03228	MS.gene35562	PPI
MS.gene62689	MS.gene03228	PPI
MS.gene35563	MS.gene03228	PPI
MS.gene55496	MS.gene03228	PPI
MS.gene003880	MS.gene03228	PPI
MS.gene54637	MS.gene03228	PPI
MS.gene49271	MS.gene03228	PPI
MS.gene67281	MS.gene03228	PPI
MS.gene62540	MS.gene03228	PPI
MS.gene025777	MS.gene03228	PPI
MS.gene022456	MS.gene03228	PPI
MS.gene062239	MS.gene03228	PPI
MS.gene46886	MS.gene03228	PPI
MS.gene03228	MS.gene21696	PPI
MS.gene21155	MS.gene03228	PPI
MS.gene92775	MS.gene03228	PPI
MS.gene03228	MS.gene29793	PPI
MS.gene03228	MS.gene074045	PPI
MS.gene09934	MS.gene03228	PPI
MS.gene03228	MS.gene85596	PPI
MS.gene21932	MS.gene03228	PPI
MS.gene94551	MS.gene03228	PPI
MS.gene03228	MS.gene64317	PPI
MS.gene47134	MS.gene03228	PPI
MS.gene056383	MS.gene03228	PPI
MS.gene022455	MS.gene03228	PPI
MS.gene87673	MS.gene03228	PPI
MS.gene05284	MS.gene03228	PPI
MS.gene041795	MS.gene03228	PPI
MS.gene03228	MS.gene93087	PPI
MS.gene27529	MS.gene03228	PPI
MS.gene046988	MS.gene03228	PPI
MS.gene03228	MS.gene057293	PPI
MS.gene22553	MS.gene03228	PPI
MS.gene80011	MS.gene03228	PPI
MS.gene96914	MS.gene03228	PPI
MS.gene53648	MS.gene03228	PPI
MS.gene070168	MS.gene03228	PPI
MS.gene017446	MS.gene03228	PPI
MS.gene03228	MS.gene79028	PPI
MS.gene016268	MS.gene03228	PPI
MS.gene038456	MS.gene03228	PPI
MS.gene03228	MS.gene026940	PPI
MS.gene060446	MS.gene03228	PPI
MS.gene004899	MS.gene03228	PPI
MS.gene03228	MS.gene98295	PPI
MS.gene80013	MS.gene03228	PPI
MS.gene25186	MS.gene03228	PPI
MS.gene03228	MS.gene96922	PPI
MS.gene008831	MS.gene03228	PPI
MS.gene96913	MS.gene03228	PPI
MS.gene009021	MS.gene03228	PPI
MS.gene03228	MS.gene025797	PPI
MS.gene03228	MS.gene21931	PPI
MS.gene03228	MS.gene64314	PPI
MS.gene03228	MS.gene53659	PPI
MS.gene070167	MS.gene03228	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03228.t1	MTR_3g088655	97.183	142	4	0	1	142	1	142	4.02e-94	269
MS.gene03228.t1	MTR_1g030440	59.859	142	49	3	1	142	15	148	1.83e-45	146
MS.gene03228.t1	MTR_4g067270	31.724	145	77	3	4	142	1	129	3.90e-13	62.8
MS.gene03228.t1	MTR_7g074240	31.034	145	88	4	4	142	20	158	4.17e-13	63.5
MS.gene03228.t1	MTR_8g036075	35.862	145	73	6	6	142	47	179	9.27e-13	62.8
MS.gene03228.t1	MTR_5g017510	30.496	141	74	5	8	142	48	170	1.14e-12	62.4
MS.gene03228.t1	MTR_5g017560	30.496	141	74	5	8	142	48	170	1.14e-12	62.4
MS.gene03228.t1	MTR_8g105230	32.117	137	81	4	6	142	34	158	6.58e-12	60.5
MS.gene03228.t1	MTR_7g089760	32.653	147	73	4	4	142	1	129	2.43e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03228.t1	AT1G21550	39.437	142	80	3	2	142	4	140	2.02e-27	100
MS.gene03228.t1	AT4G20780	36.667	150	81	3	4	142	25	171	1.00e-18	78.6
MS.gene03228.t1	AT5G44460	34.932	146	81	4	4	142	24	162	2.08e-15	69.7
MS.gene03228.t1	AT1G66400	32.639	144	75	5	5	142	12	139	7.93e-14	65.1
MS.gene03228.t1	AT2G43290	30.986	142	87	2	4	142	61	194	1.34e-13	65.5
MS.gene03228.t1	AT1G24620	34.058	138	73	5	8	142	37	159	6.41e-13	63.2
MS.gene03228.t1	AT3G59440	33.333	144	73	3	5	142	48	174	1.70e-12	62.0
MS.gene03228.t1	AT1G05990	31.724	145	78	3	4	142	1	130	1.25e-11	58.9
MS.gene03228.t1	AT5G37770	31.034	145	78	4	5	142	14	143	1.77e-11	58.9
MS.gene03228.t1	AT5G37770	31.034	145	78	4	5	142	14	143	1.77e-11	58.9

Find 20 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTATTATTGCATATTCTT+TGG	0.260505	3.4:+81654077	None:intergenic
TTCAAAGTGTTTGATTTAGA+TGG	0.287480	3.4:-81653890	MS.gene03228:CDS
GTTGAAGAGACTTGGATTCT+TGG	0.328203	3.4:-81653831	MS.gene03228:CDS
ATCAAGCCTACCATCCGAAT+TGG	0.406165	3.4:+81653760	None:intergenic
ACAAGAATTCATTGAAGTCT+AGG	0.438772	3.4:+81654008	None:intergenic
GGGATTGTAGAAGTATGATT+CGG	0.454094	3.4:-81653793	MS.gene03228:CDS
TCAACACACACTCAAGCTCT+TGG	0.480809	3.4:+81653849	None:intergenic
GTGTTTGATTTAGATGGAGA+TGG	0.487484	3.4:-81653884	MS.gene03228:CDS
TGAGAATGATGAGTTGGAGA+GGG	0.503791	3.4:-81653927	MS.gene03228:CDS
GAGAACAATGAAGAGAATAG+AGG	0.509945	3.4:-81653962	MS.gene03228:CDS
CTTGGATGAAAGTAGTGGGA+GGG	0.521104	3.4:-81653813	MS.gene03228:CDS
ATGAGAATGATGAGTTGGAG+AGG	0.535268	3.4:-81653928	MS.gene03228:CDS
TGAAGATGAGAATGATGAGT+TGG	0.536288	3.4:-81653933	MS.gene03228:CDS
GAGTGTGTGTTGAAGAGACT+TGG	0.561588	3.4:-81653839	MS.gene03228:CDS
GGATTCTTGGATGAAAGTAG+TGG	0.564575	3.4:-81653818	MS.gene03228:CDS
AACAATGAAGAGAATAGAGG+AGG	0.573830	3.4:-81653959	MS.gene03228:CDS
GATTCTTGGATGAAAGTAGT+GGG	0.605078	3.4:-81653817	MS.gene03228:CDS
GCACAGAGTTTAGCTCCTCA+AGG	0.608778	3.4:+81654101	None:intergenic
AAGCTAGATACAAATTGTGA+CGG	0.623579	3.4:-81654133	MS.gene03228:CDS
TCTTGGATGAAAGTAGTGGG+AGG	0.636199	3.4:-81653814	MS.gene03228:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGCTATTATTGCATATTCTT+TGG	+	chr3.4:81653872-81653891	None:intergenic	25.0%
!!	CTTTTACAACTCAATATCAA+AGG	-	chr3.4:81653962-81653981	MS.gene03228:CDS	25.0%
!!	TTCAAAGTGTTTGATTTAGA+TGG	-	chr3.4:81654056-81654075	MS.gene03228:CDS	25.0%
	AAGCTAGATACAAATTGTGA+CGG	-	chr3.4:81653813-81653832	MS.gene03228:CDS	30.0%
	ACAAGAATTCATTGAAGTCT+AGG	+	chr3.4:81653941-81653960	None:intergenic	30.0%
!!	TCGGTTTTATGATACCAATT+CGG	-	chr3.4:81654172-81654191	MS.gene03228:CDS	30.0%
	AACAATGAAGAGAATAGAGG+AGG	-	chr3.4:81653987-81654006	MS.gene03228:CDS	35.0%
	GAGAACAATGAAGAGAATAG+AGG	-	chr3.4:81653984-81654003	MS.gene03228:CDS	35.0%
	GATTCTTGGATGAAAGTAGT+GGG	-	chr3.4:81654129-81654148	MS.gene03228:CDS	35.0%
	TGAAGATGAGAATGATGAGT+TGG	-	chr3.4:81654013-81654032	MS.gene03228:CDS	35.0%
!	GGGATTGTAGAAGTATGATT+CGG	-	chr3.4:81654153-81654172	MS.gene03228:CDS	35.0%
!	GTGTTTGATTTAGATGGAGA+TGG	-	chr3.4:81654062-81654081	MS.gene03228:CDS	35.0%
	GGATTCTTGGATGAAAGTAG+TGG	-	chr3.4:81654128-81654147	MS.gene03228:CDS	40.0%
	GTTGAAGAGACTTGGATTCT+TGG	-	chr3.4:81654115-81654134	MS.gene03228:CDS	40.0%
!	ATGAGAATGATGAGTTGGAG+AGG	-	chr3.4:81654018-81654037	MS.gene03228:CDS	40.0%
!	TGAGAATGATGAGTTGGAGA+GGG	-	chr3.4:81654019-81654038	MS.gene03228:CDS	40.0%
	GAGTGTGTGTTGAAGAGACT+TGG	-	chr3.4:81654107-81654126	MS.gene03228:CDS	45.0%
	TCAACACACACTCAAGCTCT+TGG	+	chr3.4:81654100-81654119	None:intergenic	45.0%
!	CTTGGATGAAAGTAGTGGGA+GGG	-	chr3.4:81654133-81654152	MS.gene03228:CDS	45.0%
!	TCTTGGATGAAAGTAGTGGG+AGG	-	chr3.4:81654132-81654151	MS.gene03228:CDS	45.0%
!	GCACAGAGTTTAGCTCCTCA+AGG	+	chr3.4:81653848-81653867	None:intergenic	50.0%
!!	TGACGGTTTTGTGAGCCTTG+AGG	-	chr3.4:81653830-81653849	MS.gene03228:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	81653771	81654197	81653771	ID=MS.gene03228
chr3.4	mRNA	81653771	81654197	81653771	ID=MS.gene03228.t1;Parent=MS.gene03228
chr3.4	exon	81653771	81654197	81653771	ID=MS.gene03228.t1.exon1;Parent=MS.gene03228.t1
chr3.4	CDS	81653771	81654197	81653771	ID=cds.MS.gene03228.t1;Parent=MS.gene03228.t1

Gene Sequence

>MS.gene03228

ATGTCTATTCTATCTAGCACTGATTTGCATCGTATTTTTGAGAAGCTAGATACAAATTGTGACGGTTTTGTGAGCCTTGAGGAGCTAAACTCTGTGCTCCAAAGAATATGCAATAATAGCTCACAATTTAGCTTAGAAGAACTTGAATCTCTTGTAGAAAAGAAGAGCCTAGACTTCAATGAATTCTTGTTCTTTTACAACTCAATATCAAAGGAGAACAATGAAGAGAATAGAGGAGGTGATGAAGATGAGAATGATGAGTTGGAGAGGGATCTTGTGAAGACTTTCAAAGTGTTTGATTTAGATGGAGATGGATTCATTACAAGCCAAGAGCTTGAGTGTGTGTTGAAGAGACTTGGATTCTTGGATGAAAGTAGTGGGAGGGATTGTAGAAGTATGATTCGGTTTTATGATACCAATTCGGATG

Protein sequence

>MS.gene03228.t1

MSILSSTDLHRIFEKLDTNCDGFVSLEELNSVLQRICNNSSQFSLEELESLVEKKSLDFNEFLFFYNSISKENNEENRGGDEDENDELERDLVKTFKVFDLDGDGFITSQELECVLKRLGFLDESSGRDCRSMIRFYDTNSD