AlfalfaGEDB | MS.gene53648

M. sativa cultivar XinJiangDaYe / MS.gene53648

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53648.t1	XP_013467196.1	83.6	140	23	0	1	140	1	140	2.90E-42	181.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53648.t1	Q9LE22	47.1	138	67	1	3	140	22	153	5.6e-26	118.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53648.t1	A0A072VHE0	83.6	140	23	0	1	140	1	140	2.1e-42	181.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene036602	MS.gene53648	PPI
MS.gene27879	MS.gene53648	PPI
MS.gene040331	MS.gene53648	PPI
MS.gene58906	MS.gene53648	PPI
MS.gene53648	MS.gene21696	PPI
MS.gene58860	MS.gene53648	PPI
MS.gene53648	MS.gene022456	PPI
MS.gene53648	MS.gene53465	PPI
MS.gene27516	MS.gene53648	PPI
MS.gene53648	MS.gene074045	PPI
MS.gene053319	MS.gene53648	PPI
MS.gene04915	MS.gene53648	PPI
MS.gene53648	MS.gene85596	PPI
MS.gene61009	MS.gene53648	PPI
MS.gene53648	MS.gene96922	PPI
MS.gene64327	MS.gene53648	PPI
MS.gene000738	MS.gene53648	PPI
MS.gene64307	MS.gene53648	PPI
MS.gene53648	MS.gene025797	PPI
MS.gene061261	MS.gene53648	PPI
MS.gene04917	MS.gene53648	PPI
MS.gene53648	MS.gene64314	PPI
MS.gene53648	MS.gene94551	PPI
MS.gene036930	MS.gene53648	PPI
MS.gene013748	MS.gene53648	PPI
MS.gene53648	MS.gene046988	PPI
MS.gene55545	MS.gene53648	PPI
MS.gene53648	MS.gene057293	PPI
MS.gene53648	MS.gene53650	PPI
MS.gene53648	MS.gene02545	PPI
MS.gene008225	MS.gene53648	PPI
MS.gene53648	MS.gene79028	PPI
MS.gene64324	MS.gene53648	PPI
MS.gene011400	MS.gene53648	PPI
MS.gene024717	MS.gene53648	PPI
MS.gene53648	MS.gene026940	PPI
MS.gene53648	MS.gene65954	PPI
MS.gene64308	MS.gene53648	PPI
MS.gene53648	MS.gene98295	PPI
MS.gene64313	MS.gene53648	PPI
MS.gene35563	MS.gene53648	PPI
MS.gene97948	MS.gene53648	PPI
MS.gene55496	MS.gene53648	PPI
MS.gene003880	MS.gene53648	PPI
MS.gene54637	MS.gene53648	PPI
MS.gene49271	MS.gene53648	PPI
MS.gene67281	MS.gene53648	PPI
MS.gene62540	MS.gene53648	PPI
MS.gene53648	MS.gene21931	PPI
MS.gene53648	MS.gene001381	PPI
MS.gene025777	MS.gene53648	PPI
MS.gene53648	MS.gene87673	PPI
MS.gene53648	MS.gene53659	PPI
MS.gene53648	MS.gene09960	PPI
MS.gene062239	MS.gene53648	PPI
MS.gene46886	MS.gene53648	PPI
MS.gene53648	MS.gene061768	PPI
MS.gene53648	MS.gene64308	PPI
MS.gene21155	MS.gene53648	PPI
MS.gene53648	MS.gene47134	PPI
MS.gene92775	MS.gene53648	PPI
MS.gene53648	MS.gene017446	PPI
MS.gene53648	MS.gene80714	PPI
MS.gene53648	MS.gene96914	PPI
MS.gene53648	MS.gene96913	PPI
MS.gene21932	MS.gene53648	PPI
MS.gene28338	MS.gene53648	PPI
MS.gene027655	MS.gene53648	PPI
MS.gene056383	MS.gene53648	PPI
MS.gene53648	MS.gene057167	PPI
MS.gene53648	MS.gene93763	PPI
MS.gene53648	MS.gene49270	PPI
MS.gene022455	MS.gene53648	PPI
MS.gene05284	MS.gene53648	PPI
MS.gene015470	MS.gene53648	PPI
MS.gene041795	MS.gene53648	PPI
MS.gene63000	MS.gene53648	PPI
MS.gene22553	MS.gene53648	PPI
MS.gene53662	MS.gene53648	PPI
MS.gene53648	MS.gene026618	PPI
MS.gene53648	MS.gene29794	PPI
MS.gene53648	MS.gene008831	PPI
MS.gene53648	MS.gene55545	PPI
MS.gene53648	MS.gene71511	PPI
MS.gene53648	MS.gene033361	PPI
MS.gene53648	MS.gene056461	PPI
MS.gene038456	MS.gene53648	PPI
MS.gene53648	MS.gene89216	PPI
MS.gene53648	MS.gene35562	PPI
MS.gene53648	MS.gene47195	PPI
MS.gene004899	MS.gene53648	PPI
MS.gene53648	MS.gene060446	PPI
MS.gene53648	MS.gene07422	PPI
MS.gene009021	MS.gene53648	PPI
MS.gene53648	MS.gene023648	PPI
MS.gene53648	MS.gene64316	PPI
MS.gene53648	MS.gene29793	PPI
MS.gene53648	MS.gene98294	PPI
MS.gene87673	MS.gene53648	PPI
MS.gene53648	MS.gene03228	PPI
MS.gene074100	MS.gene53648	PPI
MS.gene53648	MS.gene46237	PPI
MS.gene53648	MS.gene64317	PPI
MS.gene065998	MS.gene53648	PPI
MS.gene63281	MS.gene53648	PPI
MS.gene68345	MS.gene53648	PPI
MS.gene44354	MS.gene53648	PPI
MS.gene001246	MS.gene53648	PPI
MS.gene53648	MS.gene93087	PPI
MS.gene040127	MS.gene53648	PPI
MS.gene62258	MS.gene53648	PPI
MS.gene53648	MS.gene64310	PPI
MS.gene53648	MS.gene29459	PPI
MS.gene53648	MS.gene71207	PPI
MS.gene000724	MS.gene53648	PPI
MS.gene74452	MS.gene53648	PPI
MS.gene53648	MS.gene74452	PPI
MS.gene53648	MS.gene09934	PPI
MS.gene64311	MS.gene53648	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53648.t1	MTR_1g047100	82.979	141	23	1	1	141	1	140	1.51e-79	231
MS.gene53648.t1	MTR_1g046950	81.690	142	26	0	1	142	1	142	5.53e-79	230
MS.gene53648.t1	MTR_5g079340	72.222	144	33	2	1	142	1	139	3.41e-68	202
MS.gene53648.t1	MTR_3g067610	62.143	140	47	1	3	142	4	137	5.06e-61	184
MS.gene53648.t1	MTR_5g008705	73.077	130	33	1	12	141	2	129	1.17e-60	183
MS.gene53648.t1	MTR_5g008695	72.174	115	32	0	28	142	1	115	5.23e-55	168
MS.gene53648.t1	MTR_8g107110	57.246	138	56	1	3	140	23	157	8.99e-51	159
MS.gene53648.t1	MTR_6g079570	55.000	140	59	2	3	141	25	161	7.35e-49	155
MS.gene53648.t1	MTR_1g032070	47.826	138	70	1	3	140	45	180	3.96e-40	133
MS.gene53648.t1	MTR_4g067270	44.286	140	74	2	3	140	4	141	3.42e-36	122
MS.gene53648.t1	MTR_8g105230	43.284	134	74	1	4	137	36	167	2.62e-35	120
MS.gene53648.t1	MTR_7g089760	46.043	139	71	2	4	140	5	141	7.42e-34	116
MS.gene53648.t1	MTR_7g075040	44.898	147	74	3	1	140	44	190	2.84e-33	115
MS.gene53648.t1	MTR_8g036075	46.853	143	70	3	4	140	49	191	2.91e-33	116
MS.gene53648.t1	MTR_6g023460	42.754	138	68	2	7	140	11	141	5.16e-32	111
MS.gene53648.t1	MTR_1g076650	40.876	137	79	1	4	140	12	146	1.41e-29	105
MS.gene53648.t1	MTR_1g076650	40.299	67	40	0	2	68	83	149	8.70e-11	56.6
MS.gene53648.t1	MTR_4g086260	41.844	141	76	2	3	142	56	191	1.52e-29	106
MS.gene53648.t1	MTR_6g025320	39.416	137	81	1	4	140	12	146	2.67e-29	104
MS.gene53648.t1	MTR_6g025320	45.312	64	35	0	79	142	12	75	4.63e-13	62.8
MS.gene53648.t1	MTR_7g087610	39.568	139	78	2	4	140	12	146	2.97e-29	104
MS.gene53648.t1	MTR_7g087610	45.312	64	35	0	79	142	12	75	5.54e-13	62.4
MS.gene53648.t1	MTR_5g088320	40.288	139	77	2	4	140	12	146	2.97e-29	104
MS.gene53648.t1	MTR_5g088320	45.312	64	35	0	79	142	12	75	4.48e-13	62.8
MS.gene53648.t1	MTR_7g090450	39.286	140	82	3	4	140	73	212	3.69e-29	105
MS.gene53648.t1	MTR_1g041285	40.876	137	79	1	4	140	12	146	3.75e-29	104
MS.gene53648.t1	MTR_2g098890	41.429	140	80	1	3	140	63	202	1.22e-28	104
MS.gene53648.t1	MTR_5g017510	40.845	142	78	3	3	142	47	184	5.28e-28	102
MS.gene53648.t1	MTR_5g017510	40.909	66	39	0	2	67	119	184	5.37e-11	58.2
MS.gene53648.t1	MTR_5g017560	40.845	142	78	3	3	142	47	184	5.28e-28	102
MS.gene53648.t1	MTR_5g017560	40.909	66	39	0	2	67	119	184	5.37e-11	58.2
MS.gene53648.t1	MTR_5g017550	38.571	140	83	1	3	142	47	183	3.83e-27	100
MS.gene53648.t1	MTR_5g017550	42.424	66	38	0	2	67	118	183	4.05e-11	58.2
MS.gene53648.t1	MTR_4g115170	39.103	156	79	3	3	142	63	218	9.98e-26	97.4
MS.gene53648.t1	MTR_8g066630	33.813	139	91	1	4	141	17	155	1.59e-25	95.1
MS.gene53648.t1	MTR_3g089070	37.226	137	77	2	7	140	9	139	3.46e-25	93.6
MS.gene53648.t1	MTR_3g089090	37.226	137	77	2	7	140	8	138	3.88e-25	93.6
MS.gene53648.t1	MTR_4g112460	31.429	140	96	0	3	142	10	149	8.80e-25	93.6
MS.gene53648.t1	MTR_4g103630	37.143	140	79	2	4	140	6	139	1.49e-24	92.0
MS.gene53648.t1	MTR_4g103650	36.429	140	80	2	4	140	2	135	3.37e-24	91.3
MS.gene53648.t1	MTR_7g074240	39.041	146	79	3	5	140	25	170	3.91e-24	92.0
MS.gene53648.t1	MTR_4g103655	36.496	137	84	1	4	140	6	139	4.41e-24	90.9
MS.gene53648.t1	MTR_1g071150	37.226	137	83	1	4	140	12	145	6.67e-24	90.5
MS.gene53648.t1	MTR_1g071150	43.750	64	36	0	1	64	81	144	5.73e-12	59.7
MS.gene53648.t1	MTR_3g083470	35.664	143	74	3	1	140	90	217	8.14e-24	92.4
MS.gene53648.t1	MTR_0168s0120	38.393	112	67	1	29	140	1	110	2.91e-22	85.5
MS.gene53648.t1	MTR_6g025320	38.393	112	67	1	29	140	1	110	2.91e-22	85.5
MS.gene53648.t1	MTR_8g069915	30.769	143	96	1	2	141	7	149	5.59e-21	83.6
MS.gene53648.t1	MTR_1g046960	66.667	60	19	1	28	87	1	59	5.89e-20	79.3
MS.gene53648.t1	MTR_5g089320	35.971	139	81	3	5	141	374	506	6.75e-20	85.1
MS.gene53648.t1	MTR_6g007613	35.036	137	87	1	4	140	29	163	1.67e-19	80.1
MS.gene53648.t1	MTR_8g078270	31.159	138	91	2	5	141	54	188	5.53e-19	79.0
MS.gene53648.t1	MTR_0028s0170	35.507	138	81	3	5	140	384	515	8.43e-19	81.6
MS.gene53648.t1	MTR_1g030440	32.877	146	84	2	3	140	21	160	1.12e-18	77.8
MS.gene53648.t1	MTR_5g009830	36.429	140	77	4	5	140	372	503	1.12e-18	81.6
MS.gene53648.t1	MTR_7g074020	33.577	137	89	1	4	140	30	164	1.37e-18	77.8
MS.gene53648.t1	MTR_7g115040	36.090	133	81	2	9	140	17	146	1.64e-18	77.0
MS.gene53648.t1	MTR_4g132070	35.000	140	79	4	5	140	397	528	1.78e-18	80.9
MS.gene53648.t1	MTR_5g092810	36.170	141	76	4	5	140	398	529	2.02e-18	80.9
MS.gene53648.t1	MTR_3g051770	36.000	150	80	4	2	140	383	527	2.04e-18	80.9
MS.gene53648.t1	MTR_8g099095	35.714	140	78	4	5	140	372	503	5.47e-18	79.3
MS.gene53648.t1	MTR_1g026190	34.286	140	80	4	5	140	388	519	4.70e-17	77.0
MS.gene53648.t1	MTR_5g027000	31.008	129	87	1	9	137	27	153	8.50e-17	72.8
MS.gene53648.t1	MTR_3g088655	30.000	150	89	4	4	140	8	154	1.56e-16	72.0
MS.gene53648.t1	MTR_5g079470	59.322	59	23	1	8	66	14	71	2.55e-16	69.3
MS.gene53648.t1	MTR_6g007613	33.333	123	80	1	4	126	29	149	1.30e-15	69.7
MS.gene53648.t1	MTR_5g011850	30.070	143	93	3	2	141	7	145	5.07e-15	67.8
MS.gene53648.t1	MTR_7g074020	32.231	121	80	1	4	124	30	148	9.35e-15	67.4
MS.gene53648.t1	MTR_4g107490	32.847	137	87	3	5	140	379	511	2.08e-14	69.3
MS.gene53648.t1	MTR_7g091890	33.333	132	85	1	9	140	368	496	3.95e-14	68.6
MS.gene53648.t1	MTR_3g055570	30.435	138	79	1	4	141	52	172	7.12e-14	65.5
MS.gene53648.t1	MTR_4g132040	33.333	141	80	4	5	140	401	532	7.50e-14	67.8
MS.gene53648.t1	MTR_7g068710	30.435	138	93	1	3	140	360	494	8.86e-13	64.7
MS.gene53648.t1	MTR_3g098090	30.000	140	85	4	5	140	338	468	1.69e-12	63.9
MS.gene53648.t1	MTR_3g098070	30.000	140	85	4	5	140	335	465	2.41e-12	63.2
MS.gene53648.t1	MTR_8g096050	28.777	139	91	1	2	140	53	183	2.88e-12	63.2
MS.gene53648.t1	MTR_1g096490	32.374	139	83	4	5	140	336	466	3.84e-12	62.8
MS.gene53648.t1	MTR_4g066660	29.286	140	91	3	3	140	376	509	3.90e-12	62.8
MS.gene53648.t1	MTR_1g054865	31.655	139	84	4	5	140	467	597	7.65e-12	62.0
MS.gene53648.t1	MTR_3g055510	30.702	114	59	2	25	138	38	131	8.13e-12	59.3
MS.gene53648.t1	MTR_5g022030	29.078	141	90	5	4	140	406	540	5.90e-11	59.3
MS.gene53648.t1	MTR_5g099240	30.769	143	80	5	5	140	330	460	6.15e-11	59.3
MS.gene53648.t1	MTR_8g095440	30.000	140	85	4	5	140	336	466	6.88e-11	59.3
MS.gene53648.t1	MTR_5g099240	30.769	143	80	5	5	140	402	532	8.10e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53648.t1	AT1G18210	54.676	139	57	1	3	141	22	154	2.16e-50	159
MS.gene53648.t1	AT1G18210	54.676	139	57	1	3	141	22	154	2.16e-50	159
MS.gene53648.t1	AT1G73630	52.857	140	60	1	2	141	18	151	9.62e-48	152
MS.gene53648.t1	AT1G66400	50.000	138	69	0	3	140	14	151	3.14e-46	147
MS.gene53648.t1	AT5G37770	49.296	142	70	1	3	142	16	157	4.32e-45	145
MS.gene53648.t1	AT5G37770	49.296	142	70	1	3	142	16	157	4.32e-45	145
MS.gene53648.t1	AT1G24620	45.652	138	73	1	4	141	37	172	3.77e-37	125
MS.gene53648.t1	AT3G07490	48.921	139	67	2	4	140	5	141	4.08e-36	122
MS.gene53648.t1	AT2G15680	43.571	140	75	1	3	142	49	184	2.27e-35	121
MS.gene53648.t1	AT1G05990	44.604	139	74	2	4	140	5	142	5.87e-35	119
MS.gene53648.t1	AT5G17470	45.000	140	71	2	5	141	3	139	9.02e-35	118
MS.gene53648.t1	AT4G03290	42.143	140	78	2	4	140	5	144	8.07e-33	113
MS.gene53648.t1	AT3G10190	43.885	139	73	3	4	140	70	205	2.51e-32	114
MS.gene53648.t1	AT3G03400	39.535	129	78	0	5	133	6	134	6.22e-32	110
MS.gene53648.t1	AT2G36180	47.407	135	68	2	8	140	4	137	8.93e-32	110
MS.gene53648.t1	AT2G43290	41.549	142	78	2	4	140	65	206	1.30e-31	112
MS.gene53648.t1	AT4G12860	43.165	139	75	2	4	140	5	141	6.85e-30	106
MS.gene53648.t1	AT5G37780	40.288	139	77	2	4	140	12	146	9.43e-30	105
MS.gene53648.t1	AT5G37780	46.032	63	34	0	79	141	12	74	5.39e-13	62.4
MS.gene53648.t1	AT1G66410	40.288	139	77	2	4	140	12	146	9.43e-30	105
MS.gene53648.t1	AT1G66410	46.032	63	34	0	79	141	12	74	5.39e-13	62.4
MS.gene53648.t1	AT3G59440	40.288	139	78	2	4	140	51	186	1.15e-29	106
MS.gene53648.t1	AT5G21274	40.146	137	80	1	4	140	12	146	1.21e-29	105
MS.gene53648.t1	AT5G21274	45.312	64	35	0	79	142	12	75	4.69e-13	62.8
MS.gene53648.t1	AT3G43810	39.416	137	81	1	4	140	12	146	2.79e-29	104
MS.gene53648.t1	AT3G43810	45.312	64	35	0	79	142	12	75	4.85e-13	62.8
MS.gene53648.t1	AT3G56800	40.288	139	77	2	4	140	12	146	2.85e-29	104
MS.gene53648.t1	AT3G56800	45.312	64	35	0	79	142	12	75	4.74e-13	62.8
MS.gene53648.t1	AT2G27030	40.288	139	77	2	4	140	12	146	2.85e-29	104
MS.gene53648.t1	AT2G27030	45.312	64	35	0	79	142	12	75	4.74e-13	62.8
MS.gene53648.t1	AT2G41110	40.288	139	77	2	4	140	12	146	2.85e-29	104
MS.gene53648.t1	AT2G41110	45.312	64	35	0	79	142	12	75	4.74e-13	62.8
MS.gene53648.t1	AT2G27030	40.288	139	77	2	4	140	12	146	8.21e-29	104
MS.gene53648.t1	AT2G27030	45.312	64	35	0	79	142	12	75	1.06e-12	62.4
MS.gene53648.t1	AT3G43810	40.288	139	77	2	4	140	12	146	1.64e-28	104
MS.gene53648.t1	AT3G43810	46.032	63	34	0	79	141	12	74	2.23e-12	62.0
MS.gene53648.t1	AT3G22930	39.416	137	81	1	4	140	35	169	1.03e-27	101
MS.gene53648.t1	AT4G14640	40.000	140	80	2	4	142	13	149	4.98e-27	99.0
MS.gene53648.t1	AT2G41410	38.028	142	81	4	4	141	70	208	4.19e-26	98.2
MS.gene53648.t1	AT2G41110	37.086	151	77	3	4	140	12	158	6.01e-26	96.3
MS.gene53648.t1	AT5G37780	36.364	154	77	3	4	140	12	161	1.52e-25	95.5
MS.gene53648.t1	AT1G32250	32.394	142	92	1	3	140	15	156	3.76e-25	94.4
MS.gene53648.t1	AT5G42380	36.232	138	87	1	3	140	48	184	4.54e-25	94.7
MS.gene53648.t1	AT2G41100	37.500	152	81	2	4	141	12	163	2.54e-24	95.9
MS.gene53648.t1	AT2G41100	36.601	153	82	2	4	141	101	253	1.95e-22	90.9
MS.gene53648.t1	AT2G41100	37.500	152	81	2	4	141	12	163	2.54e-24	95.9
MS.gene53648.t1	AT2G41100	36.601	153	82	2	4	141	101	253	1.95e-22	90.9
MS.gene53648.t1	AT5G37780	39.200	125	70	2	18	140	13	133	2.55e-24	91.3
MS.gene53648.t1	AT1G66410	39.200	125	70	2	18	140	36	156	4.34e-24	91.7
MS.gene53648.t1	AT5G37780	39.200	125	70	2	18	140	52	172	6.05e-24	91.7
MS.gene53648.t1	AT1G18530	31.250	144	96	1	1	141	1	144	1.09e-23	90.5
MS.gene53648.t1	AT1G66410	39.474	114	63	2	29	140	1	110	9.75e-23	86.7
MS.gene53648.t1	AT1G66410	39.474	114	63	2	29	140	1	110	9.75e-23	86.7
MS.gene53648.t1	AT4G20780	34.416	154	83	2	5	140	30	183	1.01e-22	89.0
MS.gene53648.t1	AT2G41100	36.601	153	82	2	4	141	66	218	1.28e-22	90.5
MS.gene53648.t1	AT2G41100	34.375	128	70	2	28	141	1	128	3.96e-16	73.2
MS.gene53648.t1	AT2G41100	36.601	153	82	2	4	141	66	218	1.28e-22	90.5
MS.gene53648.t1	AT2G41100	34.375	128	70	2	28	141	1	128	3.96e-16	73.2
MS.gene53648.t1	AT2G41100	36.601	153	82	2	4	141	66	218	1.28e-22	90.5
MS.gene53648.t1	AT2G41100	34.375	128	70	2	28	141	1	128	3.96e-16	73.2
MS.gene53648.t1	AT3G43810	38.393	112	67	1	29	140	1	110	3.04e-22	85.5
MS.gene53648.t1	AT3G43810	38.393	112	67	1	29	140	1	110	3.04e-22	85.5
MS.gene53648.t1	AT4G14640	38.889	126	73	2	18	142	9	131	3.24e-22	85.9
MS.gene53648.t1	AT4G37010	36.496	137	85	1	4	140	27	161	4.29e-22	86.7
MS.gene53648.t1	AT4G37010	36.496	137	85	1	4	140	31	165	4.47e-22	86.7
MS.gene53648.t1	AT3G50360	35.036	137	87	1	4	140	27	161	1.68e-21	85.1
MS.gene53648.t1	AT1G21550	36.184	152	79	4	3	142	9	154	3.01e-21	84.0
MS.gene53648.t1	AT2G41090	34.307	137	85	1	4	140	12	143	1.04e-20	83.6
MS.gene53648.t1	AT3G50770	30.986	142	95	2	3	141	63	204	1.19e-20	84.0
MS.gene53648.t1	AT5G44460	34.247	146	86	2	5	140	29	174	1.49e-20	83.2
MS.gene53648.t1	AT3G25600	28.986	138	98	0	4	141	12	149	2.00e-20	82.0
MS.gene53648.t1	AT4G04695	35.714	140	78	4	5	140	337	468	3.69e-20	85.5
MS.gene53648.t1	AT1G76650	34.307	137	87	1	4	140	43	176	1.20e-19	80.5
MS.gene53648.t1	AT1G76650	34.307	137	87	1	4	140	43	176	1.20e-19	80.5
MS.gene53648.t1	AT3G51920	32.168	143	90	3	2	140	7	146	1.61e-19	79.7
MS.gene53648.t1	AT4G23650	36.429	140	77	4	5	140	384	515	3.11e-19	83.2
MS.gene53648.t1	AT4G04700	35.714	140	78	4	5	140	337	468	3.64e-19	82.8
MS.gene53648.t1	AT5G19360	36.957	138	79	3	5	140	374	505	4.31e-19	82.8
MS.gene53648.t1	AT4G21940	37.143	140	76	4	5	140	407	538	1.19e-18	81.6
MS.gene53648.t1	AT4G21940	37.143	140	76	4	5	140	407	538	1.23e-18	81.3
MS.gene53648.t1	AT1G76640	32.847	137	92	0	4	140	22	158	1.32e-18	77.4
MS.gene53648.t1	AT1G50700	37.143	140	76	4	5	140	379	510	1.60e-18	81.3
MS.gene53648.t1	AT4G04720	36.429	140	77	4	5	140	385	516	3.14e-18	80.1
MS.gene53648.t1	AT4G04720	36.429	140	77	4	5	140	385	516	3.14e-18	80.1
MS.gene53648.t1	AT3G20410	37.143	140	76	4	5	140	397	528	5.28e-18	79.7
MS.gene53648.t1	AT3G20410	37.143	140	76	4	5	140	397	528	5.28e-18	79.7
MS.gene53648.t1	AT4G04740	36.429	140	77	4	5	140	374	505	5.58e-18	79.7
MS.gene53648.t1	AT4G04740	36.429	140	77	4	5	140	374	505	5.58e-18	79.7
MS.gene53648.t1	AT4G04740	36.429	140	77	4	5	140	374	505	5.58e-18	79.7
MS.gene53648.t1	AT4G04740	36.429	140	77	4	5	140	374	505	5.58e-18	79.7
MS.gene53648.t1	AT4G04740	36.429	140	77	4	5	140	374	505	5.58e-18	79.7
MS.gene53648.t1	AT4G04740	36.429	140	77	4	5	140	374	505	5.58e-18	79.7
MS.gene53648.t1	AT3G01830	36.170	141	82	5	3	140	10	145	8.34e-18	75.1
MS.gene53648.t1	AT5G12180	35.507	138	81	3	5	140	379	510	8.93e-18	79.0
MS.gene53648.t1	AT1G76650	34.307	137	77	2	4	140	43	166	1.47e-17	74.7
MS.gene53648.t1	AT3G03410	29.630	135	84	1	6	140	4	127	2.31e-17	73.6
MS.gene53648.t1	AT1G76040	35.714	140	78	4	5	140	179	310	7.13e-17	75.5
MS.gene53648.t1	AT1G76040	35.714	140	78	4	5	140	179	310	7.13e-17	75.5
MS.gene53648.t1	AT1G74740	38.849	139	76	4	5	140	365	497	1.13e-16	75.9
MS.gene53648.t1	AT1G76040	35.714	140	78	4	5	140	417	548	1.67e-16	75.5
MS.gene53648.t1	AT3G03000	31.579	133	90	1	4	136	20	151	1.90e-16	72.0
MS.gene53648.t1	AT3G03000	37.838	74	46	0	2	75	92	165	2.38e-11	58.5
MS.gene53648.t1	AT1G61950	35.507	138	81	3	5	140	405	536	2.75e-16	74.7
MS.gene53648.t1	AT5G17480	61.404	57	21	1	8	64	13	68	7.92e-16	68.2
MS.gene53648.t1	AT3G51850	31.159	138	92	1	3	140	358	492	4.97e-15	71.2
MS.gene53648.t1	AT4G04710	34.286	140	80	4	5	140	338	469	5.11e-15	70.9
MS.gene53648.t1	AT4G04710	34.286	140	80	4	5	140	324	455	5.26e-15	70.9
MS.gene53648.t1	AT4G04710	34.286	140	80	4	5	140	351	482	6.13e-15	70.9
MS.gene53648.t1	AT1G62820	31.206	141	85	3	5	141	14	146	6.37e-15	67.4
MS.gene53648.t1	AT1G18890	35.971	139	80	4	5	140	369	501	9.94e-15	70.1
MS.gene53648.t1	AT2G41100	32.000	125	70	1	32	141	4	128	2.24e-14	67.4
MS.gene53648.t1	AT3G22930	36.082	97	60	1	4	100	35	129	2.26e-14	65.9
MS.gene53648.t1	AT1G12310	30.612	147	87	4	2	141	8	146	3.24e-14	65.9
MS.gene53648.t1	AT1G50700	31.325	166	76	5	5	140	379	536	4.12e-14	68.6
MS.gene53648.t1	AT3G03430	56.140	57	24	1	8	64	13	68	4.14e-14	63.9
MS.gene53648.t1	AT4G04710	33.803	142	80	5	5	140	351	484	8.93e-14	67.4
MS.gene53648.t1	AT2G41860	31.618	136	90	1	5	140	360	492	1.17e-13	67.0
MS.gene53648.t1	AT2G17890	30.496	141	88	5	4	140	415	549	1.21e-13	67.0
MS.gene53648.t1	AT2G41860	31.618	136	90	1	5	140	360	492	1.27e-13	67.0
MS.gene53648.t1	AT3G57530	33.577	137	85	3	5	140	369	500	1.32e-13	67.0
MS.gene53648.t1	AT1G35670	32.857	140	81	4	5	140	332	462	2.23e-13	66.2
MS.gene53648.t1	AT5G66210	32.653	147	77	5	4	140	369	503	3.88e-13	65.9
MS.gene53648.t1	AT5G66210	32.653	147	77	5	4	140	369	503	3.88e-13	65.9
MS.gene53648.t1	AT4G09570	32.857	140	81	4	5	140	331	461	5.88e-13	65.1
MS.gene53648.t1	AT4G14640	36.364	99	59	2	4	101	13	108	6.25e-13	61.6
MS.gene53648.t1	AT5G12480	31.387	137	90	2	5	140	271	404	1.31e-12	63.9
MS.gene53648.t1	AT5G12480	31.387	137	90	2	5	140	365	498	1.39e-12	63.9
MS.gene53648.t1	AT4G36070	30.282	142	89	5	3	140	377	512	3.19e-12	63.2
MS.gene53648.t1	AT4G36070	30.282	142	89	5	3	140	377	512	3.52e-12	62.8
MS.gene53648.t1	AT2G17290	31.469	143	79	5	5	140	391	521	4.45e-12	62.8
MS.gene53648.t1	AT2G17290	31.469	143	79	5	5	140	391	521	4.45e-12	62.8
MS.gene53648.t1	AT5G07320	27.206	136	91	1	5	140	38	165	1.10e-11	61.6
MS.gene53648.t1	AT5G23580	28.986	138	89	3	5	140	328	458	2.25e-11	60.5
MS.gene53648.t1	AT5G51050	28.058	139	92	1	2	140	49	179	6.59e-11	59.3

Find 32 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGCTGCTGCTGCCGCTGC+TGG	0.181901	1.1:+29844731	MS.gene53648:CDS
TACTTCATCGGAAGTTGGTT+TGG	0.343185	1.1:-29844630	None:intergenic
GAAGAGATGCTTTCGAAGCT+TGG	0.383501	1.1:+29844605	MS.gene53648:CDS
TTGCTGCTGCTGCCGCTGCT+GGG	0.386164	1.1:+29844732	MS.gene53648:CDS
CACATCGTTCTTAACAAACT+TGG	0.425233	1.1:+29844830	MS.gene53648:CDS
CATCATTTGCTTTACTTCAT+CGG	0.443977	1.1:-29844642	None:intergenic
TGCTGCCGCTGCTGGGAAAG+AGG	0.481306	1.1:+29844739	MS.gene53648:CDS
TGCTTTACTTCATCGGAAGT+TGG	0.497249	1.1:-29844635	None:intergenic
CGATGAAGTAAAGCAAATGA+TGG	0.500040	1.1:+29844643	MS.gene53648:CDS
TTCAATAAGTTTGACAAGAA+CGG	0.516849	1.1:+29844554	MS.gene53648:CDS
TGAGGAGTTCAAGAAGATGA+TGG	0.534080	1.1:+29844928	MS.gene53648:CDS
GTTGCGAATCTCGTCATCCA+TGG	0.537699	1.1:-29844528	None:intergenic
AAGGTTGATGCGGATGGAGA+TGG	0.538408	1.1:+29844893	MS.gene53648:CDS
CGACCTTTACGACCTTGACA+AGG	0.551708	1.1:+29844781	MS.gene53648:CDS
AGATGGAAAAGTTAACTTTG+AGG	0.561239	1.1:+29844910	MS.gene53648:CDS
CGTCCTTGTCAAGGTCGTAA+AGG	0.562736	1.1:-29844784	None:intergenic
ATAAGCAAGGTTGATGCGGA+TGG	0.576850	1.1:+29844887	MS.gene53648:CDS
CATATTGATCAGAACGGCGA+CGG	0.579697	1.1:+29844668	MS.gene53648:CDS
TGCCGCTGCTGGGAAAGAGG+AGG	0.590208	1.1:+29844742	MS.gene53648:CDS
CTCCTCCTCTTTCCCAGCAG+CGG	0.601275	1.1:-29844744	None:intergenic
AGATGAGACCGTCCTTGTCA+AGG	0.613596	1.1:-29844793	None:intergenic
TGACGGAAAGATCTCTGTCT+CGG	0.614544	1.1:+29844577	MS.gene53648:CDS
CAGCAGCAGCAACGTTGCGG+TGG	0.616612	1.1:-29844721	None:intergenic
CATTCTCTGACAATCACTGA+GGG	0.618343	1.1:-29844864	None:intergenic
AATGATAAGCAAGGTTGATG+CGG	0.619331	1.1:+29844883	MS.gene53648:CDS
AAGTTTGACAAGAACGGTGA+CGG	0.620500	1.1:+29844560	MS.gene53648:CDS
CTTTACGACCTTGACAAGGA+CGG	0.640370	1.1:+29844785	MS.gene53648:CDS
CGGCAGCAGCAGCAACGTTG+CGG	0.643733	1.1:-29844724	None:intergenic
ATGGAACATATTGATCAGAA+CGG	0.650158	1.1:+29844662	MS.gene53648:CDS
TTGTCAGAGAATGATAAGCA+AGG	0.687328	1.1:+29844874	MS.gene53648:CDS
AAGAACGATGTGCAGTTCTG+TGG	0.688244	1.1:-29844819	None:intergenic
TCATTCTCTGACAATCACTG+AGG	0.714288	1.1:-29844865	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTCAATAAGTTTGACAAGAA+CGG	+	chr1.1:29844554-29844573	MS.gene53648:CDS	25.0%
	AGATGGAAAAGTTAACTTTG+AGG	+	chr1.1:29844910-29844929	MS.gene53648:CDS	30.0%
	ATGGAACATATTGATCAGAA+CGG	+	chr1.1:29844662-29844681	MS.gene53648:CDS	30.0%
	CATCATTTGCTTTACTTCAT+CGG	-	chr1.1:29844645-29844664	None:intergenic	30.0%
	CACATCGTTCTTAACAAACT+TGG	+	chr1.1:29844830-29844849	MS.gene53648:CDS	35.0%
	CGATGAAGTAAAGCAAATGA+TGG	+	chr1.1:29844643-29844662	MS.gene53648:CDS	35.0%
	TTGTCAGAGAATGATAAGCA+AGG	+	chr1.1:29844874-29844893	MS.gene53648:CDS	35.0%
!!	AATGATAAGCAAGGTTGATG+CGG	+	chr1.1:29844883-29844902	MS.gene53648:CDS	35.0%
	AAGTTTGACAAGAACGGTGA+CGG	+	chr1.1:29844560-29844579	MS.gene53648:CDS	40.0%
	CATTCTCTGACAATCACTGA+GGG	-	chr1.1:29844867-29844886	None:intergenic	40.0%
	TCATTCTCTGACAATCACTG+AGG	-	chr1.1:29844868-29844887	None:intergenic	40.0%
	TGAGGAGTTCAAGAAGATGA+TGG	+	chr1.1:29844928-29844947	MS.gene53648:CDS	40.0%
	TGCTTTACTTCATCGGAAGT+TGG	-	chr1.1:29844638-29844657	None:intergenic	40.0%
!	TACTTCATCGGAAGTTGGTT+TGG	-	chr1.1:29844633-29844652	None:intergenic	40.0%
	AAGAACGATGTGCAGTTCTG+TGG	-	chr1.1:29844822-29844841	None:intergenic	45.0%
	CATATTGATCAGAACGGCGA+CGG	+	chr1.1:29844668-29844687	MS.gene53648:CDS	45.0%
	CTTTACGACCTTGACAAGGA+CGG	+	chr1.1:29844785-29844804	MS.gene53648:CDS	45.0%
	GAAGAGATGCTTTCGAAGCT+TGG	+	chr1.1:29844605-29844624	MS.gene53648:CDS	45.0%
	TGACGGAAAGATCTCTGTCT+CGG	+	chr1.1:29844577-29844596	MS.gene53648:CDS	45.0%
!!	ATAAGCAAGGTTGATGCGGA+TGG	+	chr1.1:29844887-29844906	MS.gene53648:CDS	45.0%
	AGATGAGACCGTCCTTGTCA+AGG	-	chr1.1:29844796-29844815	None:intergenic	50.0%
	CGACCTTTACGACCTTGACA+AGG	+	chr1.1:29844781-29844800	MS.gene53648:CDS	50.0%
	CGTCCTTGTCAAGGTCGTAA+AGG	-	chr1.1:29844787-29844806	None:intergenic	50.0%
!!	AAGGTTGATGCGGATGGAGA+TGG	+	chr1.1:29844893-29844912	MS.gene53648:CDS	50.0%
	CTCCTCCTCTTTCCCAGCAG+CGG	-	chr1.1:29844747-29844766	None:intergenic	60.0%
	CAGCAGCAGCAACGTTGCGG+TGG	-	chr1.1:29844724-29844743	None:intergenic	65.0%
	CGGCAGCAGCAGCAACGTTG+CGG	-	chr1.1:29844727-29844746	None:intergenic	65.0%
	TGCCGCTGCTGGGAAAGAGG+AGG	+	chr1.1:29844742-29844761	MS.gene53648:CDS	65.0%
	TGCTGCCGCTGCTGGGAAAG+AGG	+	chr1.1:29844739-29844758	MS.gene53648:CDS	65.0%
	TTGCTGCTGCTGCCGCTGCT+GGG	+	chr1.1:29844732-29844751	MS.gene53648:CDS	65.0%
	GTTGCTGCTGCTGCCGCTGC+TGG	+	chr1.1:29844731-29844750	MS.gene53648:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	29844530	29844958	29844530	ID=MS.gene53648
chr1.1	mRNA	29844530	29844958	29844530	ID=MS.gene53648.t1;Parent=MS.gene53648
chr1.1	exon	29844530	29844958	29844530	ID=MS.gene53648.t1.exon1;Parent=MS.gene53648.t1
chr1.1	CDS	29844530	29844958	29844530	ID=cds.MS.gene53648.t1;Parent=MS.gene53648.t1

Gene Sequence

>MS.gene53648

ATGGATGACGAGATTCGCAACATCTTCAATAAGTTTGACAAGAACGGTGACGGAAAGATCTCTGTCTCGGAACTAGAAGAGATGCTTTCGAAGCTTGGATCCAAACCAACTTCCGATGAAGTAAAGCAAATGATGGAACATATTGATCAGAACGGCGACGGTTACATTGATCTTGAAGAATTCGCAAAATTCCACCGCAACGTTGCTGCTGCTGCCGCTGCTGGGAAAGAGGAGGAGATTCGTGATGCTTTCGACCTTTACGACCTTGACAAGGACGGTCTCATCTCTACCACAGAACTGCACATCGTTCTTAACAAACTTGGTGAGAAGTGCTCCCTCAGTGATTGTCAGAGAATGATAAGCAAGGTTGATGCGGATGGAGATGGAAAAGTTAACTTTGAGGAGTTCAAGAAGATGATGGCTCACTGA

Protein sequence

>MS.gene53648.t1

MDDEIRNIFNKFDKNGDGKISVSELEEMLSKLGSKPTSDEVKQMMEHIDQNGDGYIDLEEFAKFHRNVAAAAAAGKEEEIRDAFDLYDLDKDGLISTTELHIVLNKLGEKCSLSDCQRMISKVDADGDGKVNFEEFKKMMAH