AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene013748


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013748.t1 MTR_3g088655 97.484 159 4 0 1 159 1 159 4.13e-107 302
MS.gene013748.t1 MTR_1g030440 61.783 157 52 3 1 157 15 163 1.28e-55 172
MS.gene013748.t1 MTR_7g074240 34.568 162 94 4 4 159 20 175 6.31e-22 87.0
MS.gene013748.t1 MTR_8g036075 37.736 159 79 6 6 156 47 193 9.38e-19 79.3
MS.gene013748.t1 MTR_4g067270 33.121 157 83 3 4 154 1 141 2.30e-18 77.0
MS.gene013748.t1 MTR_5g017510 31.818 154 81 5 8 155 48 183 2.60e-17 75.1
MS.gene013748.t1 MTR_5g017560 31.818 154 81 5 8 155 48 183 2.60e-17 75.1
MS.gene013748.t1 MTR_3g067610 31.847 157 79 5 4 154 1 135 4.28e-17 73.6
MS.gene013748.t1 MTR_7g089760 33.962 159 79 4 4 154 1 141 1.63e-16 72.4
MS.gene013748.t1 MTR_8g105230 33.562 146 85 4 6 151 34 167 1.90e-16 73.2
MS.gene013748.t1 MTR_1g032070 35.135 148 84 4 8 155 46 181 2.64e-16 72.8
MS.gene013748.t1 MTR_4g115170 34.615 156 90 3 8 154 64 216 1.40e-15 71.2
MS.gene013748.t1 MTR_5g092810 33.784 148 80 4 9 154 398 529 1.55e-15 73.2
MS.gene013748.t1 MTR_1g046950 32.895 152 80 5 9 154 5 140 1.62e-15 69.3
MS.gene013748.t1 MTR_5g017550 29.605 152 86 4 8 155 48 182 2.51e-15 70.1
MS.gene013748.t1 MTR_4g103630 32.667 150 88 4 5 154 3 139 3.02e-15 68.6
MS.gene013748.t1 MTR_3g083470 30.263 152 84 4 7 158 92 221 3.17e-15 70.5
MS.gene013748.t1 MTR_6g079570 31.973 147 88 3 8 154 26 160 4.92e-15 68.9
MS.gene013748.t1 MTR_4g132070 33.108 148 81 4 9 154 397 528 1.51e-14 70.5
MS.gene013748.t1 MTR_7g075040 33.553 152 92 5 6 154 45 190 1.67e-14 68.2
MS.gene013748.t1 MTR_4g103650 32.653 147 86 4 8 154 2 135 2.46e-14 66.2
MS.gene013748.t1 MTR_4g103655 32.667 150 88 4 5 154 3 139 3.33e-14 65.9
MS.gene013748.t1 MTR_1g047100 29.252 147 93 3 8 154 4 139 6.84e-14 65.1
MS.gene013748.t1 MTR_1g076650 29.605 152 89 3 7 155 11 147 7.68e-14 65.1
MS.gene013748.t1 MTR_5g079340 33.333 153 77 6 8 154 4 137 8.01e-14 65.1
MS.gene013748.t1 MTR_1g041285 33.099 142 77 5 17 155 21 147 9.59e-14 65.1
MS.gene013748.t1 MTR_3g051770 32.895 152 83 4 9 154 389 527 1.64e-13 67.4
MS.gene013748.t1 MTR_1g026190 30.137 146 88 3 9 154 388 519 3.37e-13 66.2
MS.gene013748.t1 MTR_2g098890 34.395 157 83 3 4 154 60 202 6.02e-13 63.9
MS.gene013748.t1 MTR_8g107110 32.026 153 79 5 8 154 24 157 6.70e-13 63.2
MS.gene013748.t1 MTR_8g099095 31.507 146 86 4 9 154 372 503 1.26e-12 64.7
MS.gene013748.t1 MTR_1g055255 30.201 149 83 3 9 154 430 560 1.41e-12 64.7
MS.gene013748.t1 MTR_5g089320 28.289 152 83 4 9 154 374 505 2.86e-12 63.5
MS.gene013748.t1 MTR_1g071150 27.891 147 81 4 16 156 20 147 3.04e-12 60.8
MS.gene013748.t1 MTR_5g088320 26.316 152 94 3 7 155 11 147 5.07e-12 60.5
MS.gene013748.t1 MTR_1g096490 33.557 149 78 4 9 154 336 466 5.44e-12 62.8
MS.gene013748.t1 MTR_6g025320 26.316 152 94 3 7 155 11 147 5.58e-12 60.5
MS.gene013748.t1 MTR_6g023460 32.258 155 80 5 7 155 7 142 7.32e-12 59.7
MS.gene013748.t1 MTR_7g087610 26.316 152 94 3 7 155 11 147 7.72e-12 60.1
MS.gene013748.t1 MTR_5g009830 30.137 146 88 4 9 154 372 503 1.01e-11 62.0
MS.gene013748.t1 MTR_8g066630 30.065 153 96 4 7 159 16 157 1.46e-11 59.7
MS.gene013748.t1 MTR_4g127560 44.444 72 40 0 83 154 122 193 2.27e-11 59.7
MS.gene013748.t1 MTR_7g068710 29.936 157 77 5 8 154 361 494 2.42e-11 61.2
MS.gene013748.t1 MTR_7g011010 43.056 72 41 0 83 154 126 197 5.64e-11 58.5
MS.gene013748.t1 MTR_4g086260 29.252 147 90 4 8 154 57 189 7.93e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013748.t1 AT1G21550 41.935 155 84 3 2 155 4 153 1.75e-34 118
MS.gene013748.t1 AT4G20780 40.123 162 83 3 4 154 25 183 2.51e-26 99.0
MS.gene013748.t1 AT5G44460 38.037 163 87 4 4 159 24 179 2.57e-23 90.9
MS.gene013748.t1 AT1G66400 35.000 160 82 5 5 158 12 155 5.92e-21 84.0
MS.gene013748.t1 AT1G24620 36.364 154 80 5 8 158 37 175 1.14e-19 81.3
MS.gene013748.t1 AT2G43290 32.468 154 93 2 4 154 61 206 6.85e-19 80.1
MS.gene013748.t1 AT3G59440 34.615 156 79 3 5 154 48 186 1.21e-17 76.3
MS.gene013748.t1 AT1G05990 33.121 157 84 3 4 154 1 142 1.28e-17 75.1
MS.gene013748.t1 AT5G37770 33.121 157 83 4 5 154 14 155 1.61e-16 72.8
MS.gene013748.t1 AT5G37770 33.121 157 83 4 5 154 14 155 1.61e-16 72.8
MS.gene013748.t1 AT4G03290 33.121 157 86 3 4 154 1 144 8.57e-16 70.5
MS.gene013748.t1 AT4G04710 33.784 148 80 4 9 154 324 455 2.29e-15 72.8
MS.gene013748.t1 AT4G04710 33.784 148 80 4 9 154 351 482 2.47e-15 72.4
MS.gene013748.t1 AT4G04710 33.784 148 80 4 9 154 338 469 2.57e-15 72.4
MS.gene013748.t1 AT4G04740 32.877 146 84 3 9 154 374 505 2.81e-15 72.4
MS.gene013748.t1 AT4G04740 32.877 146 84 3 9 154 374 505 2.81e-15 72.4
MS.gene013748.t1 AT4G04740 32.877 146 84 3 9 154 374 505 2.81e-15 72.4
MS.gene013748.t1 AT4G04740 32.877 146 84 3 9 154 374 505 2.81e-15 72.4
MS.gene013748.t1 AT4G04740 32.877 146 84 3 9 154 374 505 2.81e-15 72.4
MS.gene013748.t1 AT4G04740 32.877 146 84 3 9 154 374 505 2.81e-15 72.4
MS.gene013748.t1 AT3G51850 33.758 157 71 5 8 154 359 492 4.71e-15 71.6
MS.gene013748.t1 AT4G04720 34.247 146 82 4 9 154 385 516 5.14e-15 71.6
MS.gene013748.t1 AT4G04720 34.247 146 82 4 9 154 385 516 5.14e-15 71.6
MS.gene013748.t1 AT1G76640 32.680 153 81 4 8 154 22 158 6.69e-15 68.2
MS.gene013748.t1 AT5G19360 29.530 149 85 3 9 154 374 505 8.45e-15 71.2
MS.gene013748.t1 AT5G12180 29.530 149 85 3 9 154 379 510 1.02e-14 70.9
MS.gene013748.t1 AT3G51920 30.137 146 84 4 16 158 20 150 1.44e-14 67.4
MS.gene013748.t1 AT3G07490 33.758 157 82 3 4 154 1 141 1.93e-14 67.0
MS.gene013748.t1 AT5G17470 30.405 148 89 5 9 154 3 138 2.19e-14 66.6
MS.gene013748.t1 AT4G04710 34.000 150 79 5 9 154 351 484 4.69e-14 68.9
MS.gene013748.t1 AT1G32250 31.293 147 95 2 8 154 16 156 8.24e-14 65.5
MS.gene013748.t1 AT1G73630 31.613 155 88 5 2 154 12 150 8.73e-14 65.5
MS.gene013748.t1 AT4G04700 34.932 146 81 4 9 154 337 468 1.07e-13 67.8
MS.gene013748.t1 AT4G04695 33.784 148 80 4 9 154 337 468 1.81e-13 67.4
MS.gene013748.t1 AT4G12860 29.221 154 93 2 4 154 1 141 2.03e-13 64.3
MS.gene013748.t1 AT1G35670 30.921 152 78 4 9 154 332 462 2.27e-13 67.0
MS.gene013748.t1 AT4G21940 32.192 146 85 4 9 154 407 538 2.83e-13 66.6
MS.gene013748.t1 AT4G21940 32.192 146 85 4 9 154 407 538 3.02e-13 66.6
MS.gene013748.t1 AT2G36180 29.677 155 82 5 9 154 1 137 4.65e-13 63.2
MS.gene013748.t1 AT5G42380 34.211 152 87 5 4 154 45 184 4.95e-13 63.9
MS.gene013748.t1 AT4G09570 31.579 152 77 4 9 154 331 461 1.96e-12 64.3
MS.gene013748.t1 AT5G37780 26.316 152 94 3 7 155 11 147 1.98e-12 61.6
MS.gene013748.t1 AT1G66410 26.316 152 94 3 7 155 11 147 1.98e-12 61.6
MS.gene013748.t1 AT4G23650 32.192 146 85 4 9 154 384 515 2.34e-12 63.9
MS.gene013748.t1 AT5G21274 26.974 152 93 3 7 155 11 147 2.69e-12 61.2
MS.gene013748.t1 AT1G18530 29.139 151 97 2 9 159 8 148 2.79e-12 61.2
MS.gene013748.t1 AT4G14640 28.188 149 95 2 7 155 12 148 2.79e-12 61.2
MS.gene013748.t1 AT3G47480 41.096 73 41 2 83 154 107 178 5.62e-12 61.2
MS.gene013748.t1 AT3G43810 26.316 152 94 3 7 155 11 147 5.84e-12 60.5
MS.gene013748.t1 AT3G43810 27.333 150 91 3 13 159 17 151 7.25e-12 61.2
MS.gene013748.t1 AT2G15680 29.333 150 92 4 5 154 47 182 1.06e-11 60.5
MS.gene013748.t1 AT2G38910 28.947 152 81 4 9 154 440 570 1.30e-11 62.0
MS.gene013748.t1 AT3G56800 25.658 152 95 3 7 155 11 147 1.77e-11 58.9
MS.gene013748.t1 AT2G27030 25.658 152 95 3 7 155 11 147 1.77e-11 58.9
MS.gene013748.t1 AT2G41110 25.658 152 95 3 7 155 11 147 1.77e-11 58.9
MS.gene013748.t1 AT1G50700 28.082 146 91 3 9 154 379 510 2.53e-11 61.2
MS.gene013748.t1 AT4G14640 28.358 134 84 2 22 155 9 130 3.40e-11 58.2
MS.gene013748.t1 AT1G18210 26.174 149 90 4 8 154 23 153 3.90e-11 58.5
MS.gene013748.t1 AT1G18210 26.174 149 90 4 8 154 23 153 3.90e-11 58.5
MS.gene013748.t1 AT2G27030 25.658 152 95 3 7 155 11 147 5.42e-11 58.5
MS.gene013748.t1 AT5G23580 29.530 149 84 3 9 154 328 458 6.14e-11 60.1
MS.gene013748.t1 AT5G37780 29.197 137 79 5 22 155 13 134 6.92e-11 57.4
MS.gene013748.t1 AT3G20410 28.082 146 91 3 9 154 397 528 7.46e-11 59.7
MS.gene013748.t1 AT3G20410 28.082 146 91 3 9 154 397 528 7.46e-11 59.7
MS.gene013748.t1 AT3G50770 29.299 157 94 5 5 156 61 205 7.52e-11 58.5

Find 21 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTATTATTGCATATTCTT+TGG 0.260505 3.1:-71700409 None:intergenic
TTCAAAGTGTTTGATTTAGA+TGG 0.287480 3.1:+71700596 MS.gene013748:CDS
GTTGAAGAGACTTGGATTCT+TGG 0.328203 3.1:+71700655 MS.gene013748:CDS
ATCAAGCCTACCATCCGAAT+TGG 0.406165 3.1:-71700726 None:intergenic
ACAAGAATTCATTGAAGTCT+AGG 0.438772 3.1:-71700478 None:intergenic
GGGATTGTAGAAGTATGATT+CGG 0.454094 3.1:+71700693 MS.gene013748:CDS
ATGATACCAATTCGGATGGT+AGG 0.471644 3.1:+71700720 MS.gene013748:CDS
TCAACACACACTCAAGCTCT+TGG 0.480809 3.1:-71700637 None:intergenic
GTGTTTGATTTAGATGGAGA+TGG 0.487484 3.1:+71700602 MS.gene013748:CDS
TGAGAATGATGAGTTGGAGA+GGG 0.503791 3.1:+71700559 MS.gene013748:CDS
GAGAACAATGAAGAGAATAG+AGG 0.509945 3.1:+71700524 MS.gene013748:CDS
CTTGGATGAAAGTAGTGGGA+GGG 0.521104 3.1:+71700673 MS.gene013748:CDS
ATGAGAATGATGAGTTGGAG+AGG 0.535268 3.1:+71700558 MS.gene013748:CDS
TGAAGATGAGAATGATGAGT+TGG 0.536288 3.1:+71700553 MS.gene013748:CDS
GAGTGTGTGTTGAAGAGACT+TGG 0.561588 3.1:+71700647 MS.gene013748:CDS
GGATTCTTGGATGAAAGTAG+TGG 0.564575 3.1:+71700668 MS.gene013748:CDS
AACAATGAAGAGAATAGAGG+AGG 0.573830 3.1:+71700527 MS.gene013748:CDS
GATTCTTGGATGAAAGTAGT+GGG 0.605078 3.1:+71700669 MS.gene013748:CDS
GCACAGAGTTTAGCTCCTCA+AGG 0.608778 3.1:-71700385 None:intergenic
AAGCTAGATACAAATTGTGA+CGG 0.623579 3.1:+71700353 MS.gene013748:CDS
TCTTGGATGAAAGTAGTGGG+AGG 0.636199 3.1:+71700672 MS.gene013748:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGCTATTATTGCATATTCTT+TGG - chr3.1:71700412-71700431 None:intergenic 25.0%
!! CTTTTACAACTCAATATCAA+AGG + chr3.1:71700502-71700521 MS.gene013748:CDS 25.0%
!! TTCAAAGTGTTTGATTTAGA+TGG + chr3.1:71700596-71700615 MS.gene013748:CDS 25.0%
AAGCTAGATACAAATTGTGA+CGG + chr3.1:71700353-71700372 MS.gene013748:CDS 30.0%
ACAAGAATTCATTGAAGTCT+AGG - chr3.1:71700481-71700500 None:intergenic 30.0%
! TTTTATGATACCAATTCGGA+TGG + chr3.1:71700716-71700735 MS.gene013748:CDS 30.0%
!! TCGGTTTTATGATACCAATT+CGG + chr3.1:71700712-71700731 MS.gene013748:CDS 30.0%
AACAATGAAGAGAATAGAGG+AGG + chr3.1:71700527-71700546 MS.gene013748:CDS 35.0%
GAGAACAATGAAGAGAATAG+AGG + chr3.1:71700524-71700543 MS.gene013748:CDS 35.0%
GATTCTTGGATGAAAGTAGT+GGG + chr3.1:71700669-71700688 MS.gene013748:CDS 35.0%
TGAAGATGAGAATGATGAGT+TGG + chr3.1:71700553-71700572 MS.gene013748:CDS 35.0%
! GGGATTGTAGAAGTATGATT+CGG + chr3.1:71700693-71700712 MS.gene013748:CDS 35.0%
! GTGTTTGATTTAGATGGAGA+TGG + chr3.1:71700602-71700621 MS.gene013748:CDS 35.0%
ATGATACCAATTCGGATGGT+AGG + chr3.1:71700720-71700739 MS.gene013748:CDS 40.0%
GGATTCTTGGATGAAAGTAG+TGG + chr3.1:71700668-71700687 MS.gene013748:CDS 40.0%
GTTGAAGAGACTTGGATTCT+TGG + chr3.1:71700655-71700674 MS.gene013748:CDS 40.0%
! ATGAGAATGATGAGTTGGAG+AGG + chr3.1:71700558-71700577 MS.gene013748:CDS 40.0%
! TGAGAATGATGAGTTGGAGA+GGG + chr3.1:71700559-71700578 MS.gene013748:CDS 40.0%
ATCAAGCCTACCATCCGAAT+TGG - chr3.1:71700729-71700748 None:intergenic 45.0%
GAGTGTGTGTTGAAGAGACT+TGG + chr3.1:71700647-71700666 MS.gene013748:CDS 45.0%
TCAACACACACTCAAGCTCT+TGG - chr3.1:71700640-71700659 None:intergenic 45.0%
! CTTGGATGAAAGTAGTGGGA+GGG + chr3.1:71700673-71700692 MS.gene013748:CDS 45.0%
! GGATGGTAGGCTTGATTTTC+AGG + chr3.1:71700733-71700752 MS.gene013748:CDS 45.0%
! TCTTGGATGAAAGTAGTGGG+AGG + chr3.1:71700672-71700691 MS.gene013748:CDS 45.0%
! GCACAGAGTTTAGCTCCTCA+AGG - chr3.1:71700388-71700407 None:intergenic 50.0%
!! TGACGGTTTTGTGAGCCTTG+AGG + chr3.1:71700370-71700389 MS.gene013748:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 71700311 71700790 71700311 ID=MS.gene013748
chr3.1 mRNA 71700311 71700790 71700311 ID=MS.gene013748.t1;Parent=MS.gene013748
chr3.1 exon 71700311 71700790 71700311 ID=MS.gene013748.t1.exon1;Parent=MS.gene013748.t1
chr3.1 CDS 71700311 71700790 71700311 ID=cds.MS.gene013748.t1;Parent=MS.gene013748.t1
Gene Sequence

>MS.gene013748

ATGTCTATTCTATCTAGCACTGATTTGCATCGTATTTTTGAGAAGCTAGATACAAATTGTGACGGTTTTGTGAGCCTTGAGGAGCTAAACTCTGTGCTCCAAAGAATATGCAATAATAGCTCACAATTTAGCTTAGAAGAACTTGAATCTCTTGTAGAAAAGAAGAGCCTAGACTTCAATGAATTCTTGTTCTTTTACAACTCAATATCAAAGGAGAACAATGAAGAGAATAGAGGAGGTGATGAAGATGAGAATGATGAGTTGGAGAGGGATCTTGTGAAGACTTTCAAAGTGTTTGATTTAGATGGAGATGGATTCATTACAAGCCAAGAGCTTGAGTGTGTGTTGAAGAGACTTGGATTCTTGGATGAAAGTAGTGGGAGGGATTGTAGAAGTATGATTCGGTTTTATGATACCAATTCGGATGGTAGGCTTGATTTTCAGGAATTCAAGAACATGATGTTGATTACAAGTGTATGA

Protein sequence

>MS.gene013748.t1

MSILSSTDLHRIFEKLDTNCDGFVSLEELNSVLQRICNNSSQFSLEELESLVEKKSLDFNEFLFFYNSISKENNEENRGGDEDENDELERDLVKTFKVFDLDGDGFITSQELECVLKRLGFLDESSGRDCRSMIRFYDTNSDGRLDFQEFKNMMLITSV