AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene026618


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026618.t1 MTR_1g030440 93.548 155 7 2 1 154 15 167 5.78e-98 279
MS.gene026618.t1 MTR_3g088655 64.331 157 49 3 1 150 1 157 4.70e-57 176
MS.gene026618.t1 MTR_7g074240 36.000 150 87 3 9 150 25 173 1.91e-21 85.5
MS.gene026618.t1 MTR_3g067610 34.667 150 77 3 4 147 1 135 6.36e-20 80.5
MS.gene026618.t1 MTR_8g105230 38.889 144 71 4 7 144 35 167 1.07e-19 81.3
MS.gene026618.t1 MTR_4g067270 36.842 152 77 4 4 147 1 141 2.89e-19 79.3
MS.gene026618.t1 MTR_1g046950 36.735 147 78 3 7 147 3 140 2.24e-18 76.6
MS.gene026618.t1 MTR_6g023460 37.762 143 71 3 12 148 12 142 3.13e-18 76.3
MS.gene026618.t1 MTR_1g032070 35.374 147 78 3 8 148 46 181 5.09e-18 77.0
MS.gene026618.t1 MTR_5g079340 35.374 147 77 3 7 147 3 137 1.03e-17 75.1
MS.gene026618.t1 MTR_1g047100 34.014 147 81 2 7 147 3 139 5.21e-17 73.2
MS.gene026618.t1 MTR_8g066630 33.784 148 82 3 7 147 16 154 1.09e-16 72.8
MS.gene026618.t1 MTR_6g079570 35.714 154 78 5 7 152 25 165 1.17e-16 73.2
MS.gene026618.t1 MTR_8g107110 34.014 147 79 3 7 147 23 157 7.04e-16 70.9
MS.gene026618.t1 MTR_2g098890 36.242 149 78 3 7 147 63 202 9.85e-16 71.2
MS.gene026618.t1 MTR_7g089760 34.868 152 80 3 4 147 1 141 3.43e-15 68.6
MS.gene026618.t1 MTR_4g112460 31.250 144 90 2 7 147 10 147 5.88e-15 68.6
MS.gene026618.t1 MTR_4g086260 29.730 148 97 2 7 154 56 196 7.11e-15 68.9
MS.gene026618.t1 MTR_3g083470 33.566 143 80 4 7 149 92 219 9.44e-15 68.9
MS.gene026618.t1 MTR_8g036075 34.416 154 86 4 8 152 49 196 1.38e-14 68.2
MS.gene026618.t1 MTR_7g075040 37.086 151 79 6 7 147 46 190 1.42e-14 68.2
MS.gene026618.t1 MTR_5g092810 31.788 151 84 4 9 153 398 535 1.49e-14 70.1
MS.gene026618.t1 MTR_0028s0170 31.034 145 81 4 9 147 384 515 4.05e-14 68.9
MS.gene026618.t1 MTR_4g115170 34.395 157 86 3 6 147 62 216 4.43e-14 67.4
MS.gene026618.t1 MTR_4g103650 32.192 146 81 3 8 147 2 135 7.24e-14 64.7
MS.gene026618.t1 MTR_5g009830 33.103 145 78 4 9 147 372 503 7.74e-14 68.2
MS.gene026618.t1 MTR_8g099095 32.414 145 79 4 9 147 372 503 1.55e-13 67.0
MS.gene026618.t1 MTR_5g017550 31.818 154 77 6 6 148 46 182 2.36e-13 64.7
MS.gene026618.t1 MTR_4g103655 32.192 146 81 3 8 147 6 139 4.21e-13 62.8
MS.gene026618.t1 MTR_4g132070 31.034 145 81 4 9 147 397 528 4.60e-13 65.9
MS.gene026618.t1 MTR_3g051770 31.126 151 80 4 9 147 389 527 5.57e-13 65.5
MS.gene026618.t1 MTR_1g054865 31.915 141 84 3 9 147 467 597 1.19e-12 64.7
MS.gene026618.t1 MTR_4g103630 30.822 146 83 3 8 147 6 139 1.32e-12 61.6
MS.gene026618.t1 MTR_5g017510 32.414 145 89 4 6 148 46 183 1.68e-12 62.4
MS.gene026618.t1 MTR_5g017560 32.414 145 89 4 6 148 46 183 1.68e-12 62.4
MS.gene026618.t1 MTR_5g089320 30.345 145 82 4 9 147 374 505 1.69e-12 64.3
MS.gene026618.t1 MTR_8g069915 30.714 140 94 2 8 147 12 148 3.56e-12 61.2
MS.gene026618.t1 MTR_7g090450 35.065 154 82 5 8 153 73 216 4.27e-12 61.6
MS.gene026618.t1 MTR_1g076650 29.577 142 83 3 13 148 17 147 1.23e-11 59.3
MS.gene026618.t1 MTR_1g026190 28.777 139 92 2 9 147 388 519 2.51e-11 60.8
MS.gene026618.t1 MTR_1g041285 30.872 149 82 4 8 148 12 147 2.66e-11 58.5
MS.gene026618.t1 MTR_3g098090 30.935 139 88 2 9 147 338 468 3.89e-11 60.5
MS.gene026618.t1 MTR_6g025320 28.667 150 86 5 7 148 11 147 3.97e-11 57.8
MS.gene026618.t1 MTR_5g088320 28.667 150 86 5 7 148 11 147 4.01e-11 57.8
MS.gene026618.t1 MTR_3g089070 32.414 145 80 4 9 147 7 139 7.35e-11 57.0
MS.gene026618.t1 MTR_7g087610 28.667 150 86 5 7 148 11 147 7.92e-11 57.0
MS.gene026618.t1 MTR_3g089090 32.414 145 80 4 9 147 6 138 8.70e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026618.t1 AT1G21550 46.358 151 72 5 4 148 6 153 2.27e-32 113
MS.gene026618.t1 AT4G20780 41.558 154 75 4 9 147 30 183 8.02e-27 99.8
MS.gene026618.t1 AT5G44460 40.136 147 79 3 9 147 29 174 1.10e-21 86.3
MS.gene026618.t1 AT1G24620 38.158 152 77 3 8 153 37 177 1.96e-21 85.9
MS.gene026618.t1 AT2G43290 36.242 149 81 3 7 147 64 206 1.85e-19 81.3
MS.gene026618.t1 AT5G37770 32.237 152 90 2 2 147 11 155 1.38e-17 75.1
MS.gene026618.t1 AT5G37770 32.237 152 90 2 2 147 11 155 1.38e-17 75.1
MS.gene026618.t1 AT1G05990 34.228 149 80 3 7 147 4 142 1.66e-16 72.0
MS.gene026618.t1 AT1G73630 32.414 145 89 1 3 147 15 150 1.92e-16 72.4
MS.gene026618.t1 AT1G66400 30.323 155 93 2 2 150 9 154 2.58e-16 71.6
MS.gene026618.t1 AT1G76640 34.247 146 81 2 8 147 22 158 2.64e-16 72.0
MS.gene026618.t1 AT4G12860 33.121 157 76 3 4 147 1 141 5.30e-16 70.9
MS.gene026618.t1 AT1G32250 34.722 144 89 2 7 147 15 156 1.13e-15 70.5
MS.gene026618.t1 AT3G59440 34.459 148 77 3 8 147 51 186 1.41e-15 70.9
MS.gene026618.t1 AT5G19360 31.034 145 81 3 9 147 374 505 1.68e-15 72.8
MS.gene026618.t1 AT4G23650 35.862 145 74 4 9 147 384 515 1.95e-15 72.8
MS.gene026618.t1 AT1G18530 34.722 144 83 3 8 147 7 143 4.63e-15 68.6
MS.gene026618.t1 AT4G04710 33.793 145 77 4 9 147 324 455 5.72e-15 71.2
MS.gene026618.t1 AT4G04710 33.793 145 77 4 9 147 338 469 6.24e-15 71.2
MS.gene026618.t1 AT4G04710 33.793 145 77 4 9 147 351 482 6.67e-15 71.2
MS.gene026618.t1 AT3G07490 34.868 152 80 3 4 147 1 141 1.06e-14 67.4
MS.gene026618.t1 AT4G03290 32.215 149 85 3 7 147 4 144 1.21e-14 67.4
MS.gene026618.t1 AT4G04710 33.333 147 77 4 9 147 351 484 1.25e-14 70.5
MS.gene026618.t1 AT5G12180 30.345 145 82 3 9 147 379 510 1.99e-14 69.7
MS.gene026618.t1 AT5G42380 34.722 144 90 1 4 147 45 184 2.28e-14 67.4
MS.gene026618.t1 AT5G17470 31.690 142 82 4 12 147 6 138 6.28e-14 65.5
MS.gene026618.t1 AT1G76650 34.932 146 77 3 8 147 43 176 6.95e-14 65.9
MS.gene026618.t1 AT1G76650 34.932 146 77 3 8 147 43 176 6.95e-14 65.9
MS.gene026618.t1 AT1G18210 31.757 148 76 3 8 147 23 153 1.02e-13 65.5
MS.gene026618.t1 AT1G18210 31.757 148 76 3 8 147 23 153 1.02e-13 65.5
MS.gene026618.t1 AT4G04695 31.724 145 80 4 9 147 337 468 3.13e-13 66.2
MS.gene026618.t1 AT2G15680 30.496 141 91 1 7 147 49 182 3.65e-13 64.3
MS.gene026618.t1 AT4G04700 32.414 145 79 4 9 147 337 468 4.39e-13 65.9
MS.gene026618.t1 AT1G50700 29.655 145 83 4 9 147 379 510 5.80e-13 65.5
MS.gene026618.t1 AT2G41100 29.371 143 88 3 13 143 17 158 8.71e-13 64.7
MS.gene026618.t1 AT2G41100 29.371 143 88 3 13 143 17 158 8.71e-13 64.7
MS.gene026618.t1 AT1G76650 30.667 150 68 2 8 147 43 166 1.48e-12 62.0
MS.gene026618.t1 AT1G35670 33.103 145 77 4 9 147 332 462 2.04e-12 63.9
MS.gene026618.t1 AT3G20410 30.345 145 82 4 9 147 397 528 2.04e-12 63.9
MS.gene026618.t1 AT3G20410 30.345 145 82 4 9 147 397 528 2.04e-12 63.9
MS.gene026618.t1 AT4G09570 33.099 142 81 3 9 147 331 461 2.79e-12 63.5
MS.gene026618.t1 AT4G04720 30.496 141 87 3 9 147 385 516 3.20e-12 63.5
MS.gene026618.t1 AT4G04720 30.496 141 87 3 9 147 385 516 3.20e-12 63.5
MS.gene026618.t1 AT2G38910 31.915 141 84 3 9 147 440 570 3.80e-12 63.2
MS.gene026618.t1 AT3G50770 31.126 151 94 4 4 149 60 205 3.92e-12 61.6
MS.gene026618.t1 AT4G21940 31.034 145 81 4 9 147 407 538 4.89e-12 62.8
MS.gene026618.t1 AT4G21940 31.034 145 81 4 9 147 407 538 5.32e-12 62.8
MS.gene026618.t1 AT1G50700 29.560 159 91 5 9 147 379 536 6.50e-12 62.8
MS.gene026618.t1 AT2G36180 32.168 143 81 4 12 147 4 137 8.79e-12 59.7
MS.gene026618.t1 AT4G04740 30.496 141 87 3 9 147 374 505 1.11e-11 62.0
MS.gene026618.t1 AT4G04740 30.496 141 87 3 9 147 374 505 1.11e-11 62.0
MS.gene026618.t1 AT4G04740 30.496 141 87 3 9 147 374 505 1.11e-11 62.0
MS.gene026618.t1 AT4G04740 30.496 141 87 3 9 147 374 505 1.11e-11 62.0
MS.gene026618.t1 AT4G04740 30.496 141 87 3 9 147 374 505 1.11e-11 62.0
MS.gene026618.t1 AT4G04740 30.496 141 87 3 9 147 374 505 1.11e-11 62.0
MS.gene026618.t1 AT2G41090 29.054 148 81 4 13 152 17 148 3.14e-11 58.9
MS.gene026618.t1 AT3G43810 28.667 150 86 5 7 148 11 147 4.16e-11 57.8
MS.gene026618.t1 AT5G21274 28.667 150 86 5 7 148 11 147 5.07e-11 57.8
MS.gene026618.t1 AT3G56800 28.667 150 86 5 7 148 11 147 5.69e-11 57.8
MS.gene026618.t1 AT2G27030 28.667 150 86 5 7 148 11 147 5.69e-11 57.8
MS.gene026618.t1 AT2G41110 28.667 150 86 5 7 148 11 147 5.69e-11 57.8
MS.gene026618.t1 AT3G43810 28.000 150 87 4 7 148 11 147 6.36e-11 58.5
MS.gene026618.t1 AT2G27030 28.667 150 86 5 7 148 11 147 6.47e-11 58.2
MS.gene026618.t1 AT5G37780 25.676 148 93 2 7 148 11 147 7.55e-11 57.4
MS.gene026618.t1 AT1G66410 25.676 148 93 2 7 148 11 147 7.55e-11 57.4

Find 25 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATATGATATTAATTTAGA+TGG 0.283864 1.1:-16126491 MS.gene026618:CDS
TTCAAGGTGTTTGATTTAGA+TGG 0.287480 1.1:-16126605 MS.gene026618:CDS
TCCCCTAACCCGCAATGAAT+TGG 0.338232 1.1:-16126870 MS.gene026618:CDS
CTCCAATTCATTGCGGGTTA+GGG 0.340726 1.1:+16126868 None:intergenic
GCTCCAATTCATTGCGGGTT+AGG 0.348062 1.1:+16126867 None:intergenic
AATCAATTGTTAGTGAGAAC+AGG 0.403944 1.1:-16126788 MS.gene026618:CDS
AGATGGCAAGCTTGATTTCA+AGG 0.404957 1.1:-16126474 MS.gene026618:CDS
TATCAGCTCCAATTCATTGC+GGG 0.425822 1.1:+16126862 None:intergenic
GTGTTTGATTTAGATGGAGA+TGG 0.487484 1.1:-16126599 MS.gene026618:CDS
TGGCATGTGGGATGAAGAAA+AGG 0.494222 1.1:-16126534 MS.gene026618:CDS
AAACTAGACATAAATAATGA+TGG 0.499886 1.1:-16126833 MS.gene026618:CDS
ACACCTTGAAAGCCTTCAAA+AGG 0.524154 1.1:+16126618 None:intergenic
ATTCATCACTAGTAAAGAGC+TGG 0.543089 1.1:-16126576 MS.gene026618:CDS
ACAATTGATTCAGCTCCTCA+AGG 0.548233 1.1:+16126801 None:intergenic
TCCAATTCATTGCGGGTTAG+GGG 0.573279 1.1:+16126869 None:intergenic
TGATGGTGGTGATGAGATTG+AGG 0.579256 1.1:-16126660 MS.gene026618:CDS
CAATTGATTCAGCTCCTCAA+GGG 0.587040 1.1:+16126802 None:intergenic
AGAGGAGCTTGAATATCTAG+TGG 0.598842 1.1:-16126750 MS.gene026618:CDS
GAATGTGTGTTGAAGAGACT+TGG 0.603486 1.1:-16126554 MS.gene026618:CDS
AGGTTTCAAGTATAGCATAG+AGG 0.617626 1.1:-16126768 MS.gene026618:CDS
GAGGAGCTTGAATATCTAGT+GGG 0.620423 1.1:-16126749 MS.gene026618:CDS
TGTTGAAGAGACTTGGCATG+TGG 0.646557 1.1:-16126547 MS.gene026618:CDS
GTTGAAGAGACTTGGCATGT+GGG 0.652246 1.1:-16126546 MS.gene026618:CDS
ATATCAGCTCCAATTCATTG+CGG 0.652735 1.1:+16126861 None:intergenic
TGGTGGTGATGAGATTGAGG+AGG 0.715134 1.1:-16126657 MS.gene026618:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATATGATATTAATTTAGA+TGG - chr1.1:16126817-16126836 MS.gene026618:CDS 15.0%
!! AAACTAGACATAAATAATGA+TGG - chr1.1:16126475-16126494 MS.gene026618:CDS 20.0%
! TCCATATTGAATCACAAAAA+TGG - chr1.1:16126625-16126644 MS.gene026618:CDS 25.0%
!!! ACCATTTTTGTGATTCAATA+TGG + chr1.1:16126629-16126648 None:intergenic 25.0%
AATCAATTGTTAGTGAGAAC+AGG - chr1.1:16126520-16126539 MS.gene026618:CDS 30.0%
TTGAATCACAAAAATGGTGA+TGG - chr1.1:16126631-16126650 MS.gene026618:CDS 30.0%
! ATTAGAAAGTGACCTTTTGA+AGG - chr1.1:16126678-16126697 MS.gene026618:CDS 30.0%
! TTCAAGGTGTTTGATTTAGA+TGG - chr1.1:16126703-16126722 MS.gene026618:CDS 30.0%
ATATCAGCTCCAATTCATTG+CGG + chr1.1:16126450-16126469 None:intergenic 35.0%
ATTCATCACTAGTAAAGAGC+TGG - chr1.1:16126732-16126751 MS.gene026618:CDS 35.0%
! AGGTTTCAAGTATAGCATAG+AGG - chr1.1:16126540-16126559 MS.gene026618:CDS 35.0%
! GTGTTTGATTTAGATGGAGA+TGG - chr1.1:16126709-16126728 MS.gene026618:CDS 35.0%
! TGATGGAATTTTATCCCTTG+AGG - chr1.1:16126492-16126511 MS.gene026618:CDS 35.0%
!! AATCACAAAAATGGTGATGG+TGG - chr1.1:16126634-16126653 MS.gene026618:CDS 35.0%
ACACCTTGAAAGCCTTCAAA+AGG + chr1.1:16126693-16126712 None:intergenic 40.0%
AGAGGAGCTTGAATATCTAG+TGG - chr1.1:16126558-16126577 MS.gene026618:CDS 40.0%
AGATGGCAAGCTTGATTTCA+AGG - chr1.1:16126834-16126853 MS.gene026618:CDS 40.0%
GAATGTGTGTTGAAGAGACT+TGG - chr1.1:16126754-16126773 MS.gene026618:CDS 40.0%
GAGGAGCTTGAATATCTAGT+GGG - chr1.1:16126559-16126578 MS.gene026618:CDS 40.0%
TATCAGCTCCAATTCATTGC+GGG + chr1.1:16126449-16126468 None:intergenic 40.0%
! ACAATTGATTCAGCTCCTCA+AGG + chr1.1:16126510-16126529 None:intergenic 40.0%
! CAATTGATTCAGCTCCTCAA+GGG + chr1.1:16126509-16126528 None:intergenic 40.0%
!! TGACCTTTTGAAGGCTTTCA+AGG - chr1.1:16126687-16126706 MS.gene026618:CDS 40.0%
GTTGAAGAGACTTGGCATGT+GGG - chr1.1:16126762-16126781 MS.gene026618:CDS 45.0%
TGGCATGTGGGATGAAGAAA+AGG - chr1.1:16126774-16126793 MS.gene026618:CDS 45.0%
TGTTGAAGAGACTTGGCATG+TGG - chr1.1:16126761-16126780 MS.gene026618:CDS 45.0%
! CTCCAATTCATTGCGGGTTA+GGG + chr1.1:16126443-16126462 None:intergenic 45.0%
!! TCCAATTCATTGCGGGTTAG+GGG + chr1.1:16126442-16126461 None:intergenic 45.0%
!! TGATGGTGGTGATGAGATTG+AGG - chr1.1:16126648-16126667 MS.gene026618:CDS 45.0%
GCTCCAATTCATTGCGGGTT+AGG + chr1.1:16126444-16126463 None:intergenic 50.0%
TCCCCTAACCCGCAATGAAT+TGG - chr1.1:16126438-16126457 MS.gene026618:CDS 50.0%
! TGGTGGTGATGAGATTGAGG+AGG - chr1.1:16126651-16126670 MS.gene026618:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 16126433 16126897 16126433 ID=MS.gene026618
chr1.1 mRNA 16126433 16126897 16126433 ID=MS.gene026618.t1;Parent=MS.gene026618
chr1.1 exon 16126433 16126897 16126433 ID=MS.gene026618.t1.exon1;Parent=MS.gene026618.t1
chr1.1 CDS 16126433 16126897 16126433 ID=cds.MS.gene026618.t1;Parent=MS.gene026618.t1
Gene Sequence

>MS.gene026618

ATGTGTCCCCTAACCCGCAATGAATTGGAGCTGATATTTAAGAAACTAGACATAAATAATGATGGAATTTTATCCCTTGAGGAGCTGAATCAATTGTTAGTGAGAACAGGTTTCAAGTATAGCATAGAGGAGCTTGAATATCTAGTGGGAAAGAAGAGTCTAAACTTGAGTGAGTTCTTATGCTTTTATGACTCCATATTGAATCACAAAAATGGTGATGGTGGTGATGAGATTGAGGAGGTAGAATTAGAAAGTGACCTTTTGAAGGCTTTCAAGGTGTTTGATTTAGATGGAGATGGATTCATCACTAGTAAAGAGCTGGAATGTGTGTTGAAGAGACTTGGCATGTGGGATGAAGAAAAGGATTGTAGATCAATGATTTATTCATATGATATTAATTTAGATGGCAAGCTTGATTTCAAGGAATTTAAGAACATGATGTTGCTTACACTACAACGTGTTTAG

Protein sequence

>MS.gene026618.t1

MCPLTRNELELIFKKLDINNDGILSLEELNQLLVRTGFKYSIEELEYLVGKKSLNLSEFLCFYDSILNHKNGDGGDEIEEVELESDLLKAFKVFDLDGDGFITSKELECVLKRLGMWDEEKDCRSMIYSYDINLDGKLDFKEFKNMMLLTLQRV