AlfalfaGEDB | MS.gene21155

M. sativa cultivar XinJiangDaYe / MS.gene21155

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF00036.35 PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21155.t1	XP_003611244.1	97.8	135	3	0	1	135	13	147	5.60E-68	266.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21155.t1	Q94AZ4	86.7	135	18	0	1	135	14	148	4.6e-63	241.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21155.t1	B7FNA2	97.8	135	3	0	1	135	13	147	4.0e-68	266.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene21155	MS.gene62540	PPI
MS.gene68345	MS.gene21155	PPI
MS.gene21155	MS.gene046988	PPI
MS.gene21155	MS.gene074100	PPI
MS.gene21155	MS.gene53650	PPI
MS.gene21155	MS.gene02545	PPI
MS.gene21155	MS.gene04915	PPI
MS.gene62258	MS.gene21155	PPI
MS.gene21155	MS.gene61009	PPI
MS.gene21155	MS.gene58906	PPI
MS.gene21155	MS.gene35563	PPI
MS.gene21155	MS.gene64311	PPI
MS.gene21155	MS.gene026940	PPI
MS.gene21155	MS.gene98295	PPI
MS.gene21155	MS.gene64327	PPI
MS.gene21155	MS.gene28338	PPI
MS.gene21155	MS.gene041795	PPI
MS.gene58860	MS.gene21155	PPI
MS.gene21155	MS.gene21931	PPI
MS.gene21155	MS.gene033361	PPI
MS.gene21155	MS.gene87673	PPI
MS.gene97948	MS.gene21155	PPI
MS.gene21155	MS.gene27516	PPI
MS.gene21155	MS.gene040127	PPI
MS.gene21155	MS.gene53659	PPI
MS.gene21155	MS.gene09960	PPI
MS.gene04917	MS.gene21155	PPI
MS.gene21155	MS.gene061768	PPI
MS.gene21155	MS.gene89216	PPI
MS.gene21155	MS.gene64308	PPI
MS.gene21155	MS.gene47195	PPI
MS.gene21155	MS.gene47134	PPI
MS.gene21155	MS.gene017446	PPI
MS.gene21155	MS.gene96914	PPI
MS.gene21155	MS.gene96913	PPI
MS.gene21155	MS.gene057167	PPI
MS.gene21155	MS.gene93763	PPI
MS.gene21155	MS.gene64313	PPI
MS.gene21155	MS.gene49270	PPI
MS.gene21155	MS.gene64307	PPI
MS.gene64324	MS.gene21155	PPI
MS.gene21155	MS.gene024717	PPI
MS.gene011400	MS.gene21155	PPI
MS.gene21155	MS.gene036930	PPI
MS.gene21155	MS.gene21932	PPI
MS.gene21155	MS.gene026618	PPI
MS.gene21155	MS.gene29794	PPI
MS.gene21155	MS.gene53648	PPI
MS.gene21155	MS.gene008831	PPI
MS.gene21155	MS.gene55545	PPI
MS.gene21155	MS.gene71511	PPI
MS.gene21155	MS.gene056461	PPI
MS.gene003880	MS.gene21155	PPI
MS.gene54637	MS.gene21155	PPI
MS.gene67281	MS.gene21155	PPI
MS.gene21155	MS.gene29459	PPI
MS.gene21155	MS.gene35562	PPI
MS.gene21155	MS.gene009021	PPI
MS.gene46886	MS.gene21155	PPI
MS.gene21155	MS.gene63281	PPI
MS.gene21155	MS.gene060446	PPI
MS.gene21155	MS.gene07422	PPI
MS.gene21155	MS.gene062239	PPI
MS.gene21155	MS.gene013748	PPI
MS.gene21155	MS.gene64316	PPI
MS.gene21155	MS.gene29793	PPI
MS.gene21155	MS.gene98294	PPI
MS.gene21155	MS.gene05284	PPI
MS.gene21155	MS.gene22553	PPI
MS.gene21155	MS.gene061261	PPI
MS.gene21155	MS.gene03228	PPI
MS.gene21155	MS.gene000738	PPI
MS.gene022455	MS.gene21155	PPI
MS.gene21155	MS.gene46237	PPI
MS.gene21155	MS.gene64317	PPI
MS.gene21155	MS.gene040331	PPI
MS.gene21155	MS.gene015470	PPI
MS.gene21155	MS.gene63000	PPI
MS.gene21155	MS.gene93087	PPI
MS.gene21155	MS.gene64310	PPI
MS.gene21155	MS.gene057293	PPI
MS.gene21155	MS.gene71207	PPI
MS.gene21155	MS.gene92775	PPI
MS.gene21155	MS.gene74452	PPI
MS.gene21155	MS.gene09934	PPI
MS.gene038456	MS.gene21155	PPI
MS.gene21155	MS.gene79028	PPI
MS.gene21155	MS.gene53662	PPI
MS.gene004899	MS.gene21155	PPI
MS.gene21155	MS.gene49271	PPI
MS.gene21155	MS.gene053319	PPI
MS.gene21155	MS.gene008225	PPI
MS.gene21155	MS.gene000724	PPI
MS.gene21155	MS.gene65954	PPI
MS.gene21155	MS.gene21696	PPI
MS.gene21155	MS.gene022456	PPI
MS.gene21155	MS.gene001246	PPI
MS.gene21155	MS.gene074045	PPI
MS.gene21155	MS.gene85596	PPI
MS.gene21155	MS.gene96922	PPI
MS.gene21155	MS.gene55496	PPI
MS.gene21155	MS.gene025797	PPI
MS.gene21155	MS.gene056383	PPI
MS.gene21155	MS.gene64314	PPI
MS.gene21155	MS.gene025777	PPI
MS.gene21155	MS.gene94551	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21155.t1	MTR_5g011850	97.778	135	3	0	1	135	13	147	8.70e-95	270
MS.gene21155.t1	MTR_5g088320	50.735	136	65	1	2	135	14	149	7.78e-44	141
MS.gene21155.t1	MTR_6g025320	50.735	136	65	1	2	135	14	149	1.06e-43	141
MS.gene21155.t1	MTR_7g087610	50.000	136	66	1	2	135	14	149	1.64e-43	140
MS.gene21155.t1	MTR_1g041285	42.647	136	76	1	1	134	13	148	4.91e-36	122
MS.gene21155.t1	MTR_1g076650	40.741	135	78	1	2	134	14	148	1.18e-34	118
MS.gene21155.t1	MTR_0168s0120	48.673	113	56	1	25	135	1	113	1.70e-33	114
MS.gene21155.t1	MTR_6g025320	48.673	113	56	1	25	135	1	113	1.70e-33	114
MS.gene21155.t1	MTR_1g071150	35.714	140	82	3	3	140	15	148	2.35e-26	97.4
MS.gene21155.t1	MTR_7g115040	37.313	134	82	1	3	134	15	148	1.78e-22	87.4
MS.gene21155.t1	MTR_8g066630	34.586	133	82	3	1	128	18	150	1.83e-21	85.1
MS.gene21155.t1	MTR_1g032070	33.824	136	88	1	1	134	47	182	9.65e-20	81.6
MS.gene21155.t1	MTR_8g105230	33.846	130	84	1	1	128	37	166	8.04e-19	79.0
MS.gene21155.t1	MTR_7g074020	31.343	134	90	1	1	132	31	164	1.81e-18	77.4
MS.gene21155.t1	MTR_6g007613	31.343	134	90	1	1	132	30	163	6.10e-18	76.3
MS.gene21155.t1	MTR_5g079340	33.824	136	85	3	1	133	5	138	4.82e-17	73.2
MS.gene21155.t1	MTR_4g107490	33.582	134	86	2	1	132	379	511	7.36e-17	76.6
MS.gene21155.t1	MTR_4g112460	31.618	136	89	2	1	132	12	147	1.22e-16	72.8
MS.gene21155.t1	MTR_7g074020	31.356	118	79	1	1	116	31	148	2.00e-16	72.0
MS.gene21155.t1	MTR_8g107110	33.588	131	82	2	1	128	25	153	3.22e-16	71.6
MS.gene21155.t1	MTR_6g007613	31.356	118	79	1	1	116	30	147	6.03e-16	70.9
MS.gene21155.t1	MTR_1g096490	30.597	134	88	3	1	132	336	466	2.66e-15	72.0
MS.gene21155.t1	MTR_3g055570	36.842	133	63	3	1	129	53	168	5.85e-15	68.6
MS.gene21155.t1	MTR_1g046950	30.657	137	91	2	1	133	5	141	6.29e-15	67.8
MS.gene21155.t1	MTR_6g079570	32.624	141	85	4	1	135	27	163	7.70e-15	68.2
MS.gene21155.t1	MTR_1g047100	29.197	137	94	2	1	134	5	141	1.54e-14	66.6
MS.gene21155.t1	MTR_1g055255	32.836	134	85	3	1	132	430	560	3.66e-14	68.9
MS.gene21155.t1	MTR_7g106710	29.851	134	89	3	1	132	441	571	4.68e-14	68.6
MS.gene21155.t1	MTR_3g051770	34.286	140	83	3	1	132	389	527	9.63e-14	67.8
MS.gene21155.t1	MTR_5g027000	27.737	137	95	2	3	137	25	159	9.83e-14	65.1
MS.gene21155.t1	MTR_3g067610	29.231	130	86	2	1	128	6	131	2.94e-13	63.2
MS.gene21155.t1	MTR_7g068710	31.343	134	89	2	1	132	362	494	3.30e-13	66.2
MS.gene21155.t1	MTR_4g067270	30.882	136	90	2	1	132	6	141	4.96e-13	62.8
MS.gene21155.t1	MTR_1g041150	30.597	134	88	3	1	132	424	554	6.47e-13	65.5
MS.gene21155.t1	MTR_7g075040	31.690	142	86	3	2	132	49	190	1.95e-12	62.4
MS.gene21155.t1	MTR_1g054865	31.343	134	87	3	1	132	467	597	2.11e-12	63.9
MS.gene21155.t1	MTR_8g069915	25.161	155	109	3	1	148	13	167	3.18e-12	61.2
MS.gene21155.t1	MTR_8g095440	28.358	134	91	3	1	132	336	466	3.63e-12	63.2
MS.gene21155.t1	MTR_3g098070	27.612	134	92	3	1	132	335	465	4.22e-12	62.8
MS.gene21155.t1	MTR_3g098090	27.612	134	92	3	1	132	338	468	5.28e-12	62.8
MS.gene21155.t1	MTR_8g036075	31.915	141	86	3	2	132	51	191	6.56e-12	60.8
MS.gene21155.t1	MTR_1g052530	29.412	136	93	2	1	134	358	492	8.99e-12	62.0
MS.gene21155.t1	MTR_5g092810	31.429	140	92	3	1	138	398	535	1.09e-11	61.6
MS.gene21155.t1	MTR_3g083470	31.250	128	80	2	1	128	94	213	1.18e-11	60.5
MS.gene21155.t1	MTR_5g099240	28.358	134	91	3	1	132	402	532	1.89e-11	61.2
MS.gene21155.t1	MTR_7g091890	30.147	136	92	2	1	134	364	498	2.04e-11	60.8
MS.gene21155.t1	MTR_5g099240	28.358	134	91	3	1	132	330	460	2.08e-11	60.8
MS.gene21155.t1	MTR_4g103650	31.538	130	80	4	4	128	6	131	5.55e-11	57.0
MS.gene21155.t1	MTR_4g103655	31.538	130	80	4	4	128	10	135	5.57e-11	57.4
MS.gene21155.t1	MTR_4g132070	32.090	134	87	3	1	132	397	528	5.78e-11	59.7
MS.gene21155.t1	MTR_5g008705	26.923	130	93	1	7	134	1	130	7.19e-11	56.6
MS.gene21155.t1	MTR_4g115170	30.405	148	83	3	1	128	65	212	8.99e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21155.t1	AT1G12310	86.667	135	18	0	1	135	14	148	1.65e-84	244
MS.gene21155.t1	AT1G62820	83.704	135	22	0	1	135	14	148	7.25e-83	240
MS.gene21155.t1	AT5G21274	50.735	136	65	1	2	135	14	149	7.64e-44	141
MS.gene21155.t1	AT3G43810	50.735	136	65	1	2	135	14	149	1.11e-43	141
MS.gene21155.t1	AT5G37780	50.735	136	65	1	2	135	14	149	2.30e-43	140
MS.gene21155.t1	AT1G66410	50.735	136	65	1	2	135	14	149	2.30e-43	140
MS.gene21155.t1	AT3G56800	50.000	136	66	1	2	135	14	149	3.33e-43	140
MS.gene21155.t1	AT2G27030	50.000	136	66	1	2	135	14	149	3.33e-43	140
MS.gene21155.t1	AT2G41110	50.000	136	66	1	2	135	14	149	3.33e-43	140
MS.gene21155.t1	AT2G27030	50.000	136	66	1	2	135	14	149	6.30e-43	140
MS.gene21155.t1	AT3G43810	49.275	138	68	1	2	137	14	151	1.88e-42	140
MS.gene21155.t1	AT2G41110	45.946	148	66	2	2	135	14	161	1.42e-39	131
MS.gene21155.t1	AT5G37780	45.695	151	65	2	2	135	14	164	4.18e-39	130
MS.gene21155.t1	AT5G37780	42.593	162	65	2	2	135	14	175	4.21e-37	125
MS.gene21155.t1	AT1G66410	47.260	146	65	2	2	135	14	159	6.23e-37	124
MS.gene21155.t1	AT5G37780	48.387	124	62	1	14	135	13	136	2.24e-36	122
MS.gene21155.t1	AT3G22930	41.791	134	76	1	2	133	37	170	8.81e-36	122
MS.gene21155.t1	AT4G14640	40.299	134	78	1	2	133	15	148	1.74e-34	118
MS.gene21155.t1	AT3G43810	48.673	113	56	1	25	135	1	113	1.79e-33	114
MS.gene21155.t1	AT3G43810	48.673	113	56	1	25	135	1	113	1.79e-33	114
MS.gene21155.t1	AT1G66410	48.673	113	56	1	25	135	1	113	4.09e-33	113
MS.gene21155.t1	AT1G66410	48.673	113	56	1	25	135	1	113	4.09e-33	113
MS.gene21155.t1	AT4G14640	36.885	122	75	1	14	133	9	130	1.43e-27	100
MS.gene21155.t1	AT2G41100	35.714	168	86	4	2	150	68	232	5.94e-25	97.1
MS.gene21155.t1	AT2G41100	29.688	128	72	3	24	133	1	128	2.56e-11	60.5
MS.gene21155.t1	AT2G41100	35.714	168	86	4	2	150	68	232	5.94e-25	97.1
MS.gene21155.t1	AT2G41100	29.688	128	72	3	24	133	1	128	2.56e-11	60.5
MS.gene21155.t1	AT2G41100	35.714	168	86	4	2	150	68	232	5.94e-25	97.1
MS.gene21155.t1	AT2G41100	29.688	128	72	3	24	133	1	128	2.56e-11	60.5
MS.gene21155.t1	AT2G41100	35.714	168	86	4	2	150	103	267	9.63e-25	97.1
MS.gene21155.t1	AT2G41100	32.667	150	83	3	2	133	14	163	2.36e-19	82.8
MS.gene21155.t1	AT2G41100	35.714	168	86	4	2	150	103	267	9.63e-25	97.1
MS.gene21155.t1	AT2G41100	32.667	150	83	3	2	133	14	163	2.36e-19	82.8
MS.gene21155.t1	AT2G41090	36.765	136	81	2	2	135	14	146	8.56e-22	86.7
MS.gene21155.t1	AT4G14640	42.553	94	52	1	2	93	15	108	2.01e-21	84.0
MS.gene21155.t1	AT3G22930	41.935	93	52	1	2	92	37	129	1.05e-20	82.4
MS.gene21155.t1	AT3G50360	31.343	134	90	1	1	132	28	161	1.40e-19	80.5
MS.gene21155.t1	AT4G37010	32.836	134	88	1	1	132	28	161	8.06e-19	78.6
MS.gene21155.t1	AT4G37010	32.836	134	88	1	1	132	32	165	8.08e-19	78.6
MS.gene21155.t1	AT2G41100	34.483	145	73	4	25	150	1	142	2.75e-18	77.8
MS.gene21155.t1	AT3G51920	31.343	134	90	1	3	134	15	148	5.58e-18	75.9
MS.gene21155.t1	AT1G24620	32.867	143	94	1	1	141	38	180	6.28e-18	76.6
MS.gene21155.t1	AT1G32250	30.882	136	86	3	1	128	17	152	1.83e-17	74.7
MS.gene21155.t1	AT1G74740	35.766	137	83	4	1	134	365	499	3.11e-16	74.7
MS.gene21155.t1	AT1G18530	32.374	139	89	3	1	135	8	145	3.76e-15	68.6
MS.gene21155.t1	AT1G35670	32.090	134	86	3	1	132	332	462	5.01e-15	71.2
MS.gene21155.t1	AT1G76040	33.582	134	85	3	1	132	179	310	5.31e-15	70.9
MS.gene21155.t1	AT1G76040	33.582	134	85	3	1	132	179	310	5.31e-15	70.9
MS.gene21155.t1	AT3G03000	32.847	137	89	2	1	134	21	157	6.22e-15	68.2
MS.gene21155.t1	AT1G76040	33.582	134	85	3	1	132	417	548	6.26e-15	71.2
MS.gene21155.t1	AT4G09570	31.343	134	87	3	1	132	331	461	2.40e-14	69.3
MS.gene21155.t1	AT1G18210	29.323	133	92	1	1	133	24	154	3.27e-14	66.6
MS.gene21155.t1	AT1G18210	29.323	133	92	1	1	133	24	154	3.27e-14	66.6
MS.gene21155.t1	AT1G66400	30.657	137	91	2	1	133	16	152	4.04e-14	65.9
MS.gene21155.t1	AT5G23580	29.851	134	89	3	1	132	328	458	3.67e-13	65.9
MS.gene21155.t1	AT4G38230	29.104	134	90	3	1	132	360	490	7.57e-13	65.1
MS.gene21155.t1	AT4G38230	29.104	134	90	3	1	132	330	460	7.69e-13	65.1
MS.gene21155.t1	AT4G38230	29.104	134	90	3	1	132	330	460	7.69e-13	65.1
MS.gene21155.t1	AT4G38230	28.378	148	97	4	1	142	186	330	9.07e-13	64.7
MS.gene21155.t1	AT3G10660	31.343	134	87	3	1	132	492	622	1.15e-12	64.7
MS.gene21155.t1	AT3G10660	31.343	134	87	3	1	132	492	622	1.15e-12	64.7
MS.gene21155.t1	AT1G05990	30.827	133	87	3	1	128	6	138	1.26e-12	62.0
MS.gene21155.t1	AT3G59440	31.884	138	85	4	1	132	52	186	1.59e-12	62.4
MS.gene21155.t1	AT2G43290	29.577	142	89	4	1	132	66	206	3.08e-12	62.0
MS.gene21155.t1	AT3G20410	31.343	134	88	3	1	132	397	528	5.51e-12	62.8
MS.gene21155.t1	AT3G20410	31.343	134	88	3	1	132	397	528	5.51e-12	62.8
MS.gene21155.t1	AT2G17290	28.358	134	91	3	1	132	391	521	5.63e-12	62.8
MS.gene21155.t1	AT2G17290	28.358	134	91	3	1	132	391	521	5.63e-12	62.8
MS.gene21155.t1	AT2G15680	28.358	134	92	2	1	132	51	182	6.43e-12	60.8
MS.gene21155.t1	AT5G04870	29.851	134	89	3	1	132	456	586	7.52e-12	62.4
MS.gene21155.t1	AT2G38910	29.851	134	89	3	1	132	440	570	7.61e-12	62.4
MS.gene21155.t1	AT2G41860	30.597	134	90	2	1	132	360	492	8.07e-12	62.0
MS.gene21155.t1	AT4G03290	30.370	135	87	3	1	128	6	140	8.30e-12	59.7
MS.gene21155.t1	AT2G41860	30.597	134	90	2	1	132	360	492	8.47e-12	62.0
MS.gene21155.t1	AT1G73630	26.923	130	89	2	1	128	21	146	9.27e-12	59.7
MS.gene21155.t1	AT4G21940	32.090	134	87	3	1	132	407	538	1.14e-11	61.6
MS.gene21155.t1	AT4G21940	32.090	134	87	3	1	132	407	538	1.24e-11	61.6
MS.gene21155.t1	AT4G35310	29.104	134	90	3	1	132	403	533	2.64e-11	60.8
MS.gene21155.t1	AT4G35310	29.104	134	90	3	1	132	403	533	2.64e-11	60.8
MS.gene21155.t1	AT3G57530	30.935	139	82	4	1	132	369	500	3.24e-11	60.5
MS.gene21155.t1	AT1G18890	29.927	137	91	4	1	134	369	503	3.61e-11	60.5
MS.gene21155.t1	AT2G41100	32.500	80	51	1	71	150	12	88	3.88e-11	57.8
MS.gene21155.t1	AT4G23650	30.597	134	89	3	1	132	384	515	5.87e-11	59.7
MS.gene21155.t1	AT3G25600	26.846	149	102	3	1	142	13	161	6.65e-11	57.4
MS.gene21155.t1	AT5G12480	29.286	140	89	4	1	134	365	500	8.15e-11	59.3

Find 36 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTTCAGGTTTCATGTGTT+TGG	0.324663	5.1:+5541726	None:intergenic
CACATTGCATTGCATGTACT+TGG	0.334219	5.1:+5541504	None:intergenic
CTGAGTTTGATGAATGGATT+AGG	0.334899	5.1:-5541588	MS.gene21155:CDS
AGTTGATGATCGAACGGTTC+AGG	0.354968	5.1:+5541712	None:intergenic
TCAGATTTCTCAACAGAAAC+AGG	0.371452	5.1:-5540667	MS.gene21155:intron
GAAGTTGATGTTGGGTTTGA+TGG	0.388552	5.1:-5541565	MS.gene21155:CDS
CGATGGATTTGAGCTGAGCT+TGG	0.412141	5.1:+5541806	None:intergenic
TGCGATCTTGCCGTCGTTGT+CGG	0.415377	5.1:+5541876	None:intergenic
GGATTAGGGAAGTTGATGTT+GGG	0.442492	5.1:-5541573	MS.gene21155:CDS
TGGATTAGGGAAGTTGATGT+TGG	0.452504	5.1:-5541574	MS.gene21155:CDS
GTTCTCGATAAAGATGCAAC+TGG	0.460205	5.1:-5541673	MS.gene21155:CDS
ATTTGAGCTGAGCTTGGGTC+GGG	0.477294	5.1:+5541812	None:intergenic
AGCCTGCTGAGTTTGATGAA+TGG	0.478011	5.1:-5541594	MS.gene21155:CDS
TGAGTTTGATGAATGGATTA+GGG	0.481850	5.1:-5541587	MS.gene21155:CDS
AACCGTTCGATCATCAACTA+AGG	0.488078	5.1:-5541708	MS.gene21155:CDS
GATGGATTTGAGCTGAGCTT+GGG	0.498752	5.1:+5541807	None:intergenic
TGAAGATTTCATCGCTAGAA+TGG	0.506739	5.1:-5541530	MS.gene21155:CDS
AGGGGAGGTTAGATTCTCTT+CGG	0.513129	5.1:+5541780	None:intergenic
ATCCATTCATCAAACTCAGC+AGG	0.514616	5.1:+5541592	None:intergenic
TAGATTCTCTTCGGCGACGA+TGG	0.518627	5.1:+5541789	None:intergenic
GATTTGAGCTGAGCTTGGGT+CGG	0.523421	5.1:+5541811	None:intergenic
GGAATCCTCATGCGTTCTCT+CGG	0.535690	5.1:-5541841	MS.gene21155:CDS
AAGTTGATGTTGGGTTTGAT+GGG	0.546897	5.1:-5541564	MS.gene21155:CDS
CGACGGCAAGATCGCACCAT+CGG	0.559292	5.1:-5541869	MS.gene21155:CDS
CTCAACAGAAACAGGACAAG+TGG	0.561226	5.1:-5540659	MS.gene21155:CDS
GAAATCGAGAGAAATCGAAA+GGG	0.564111	5.1:+5541761	None:intergenic
AAGATCGCACCATCGGAGCT+CGG	0.573545	5.1:-5541862	MS.gene21155:CDS
AGAAATCGAGAGAAATCGAA+AGG	0.584540	5.1:+5541760	None:intergenic
TCCCTTAGTTGATGATCGAA+CGG	0.595563	5.1:+5541706	None:intergenic
ATGAGGATTCCGAGCTCCGA+TGG	0.601818	5.1:+5541853	None:intergenic
ATCCTCATGCGTTCTCTCGG+CGG	0.614038	5.1:-5541838	MS.gene21155:CDS
ACCGTTCGATCATCAACTAA+GGG	0.620555	5.1:-5541707	MS.gene21155:CDS
TACCGCCGAGAGAACGCATG+AGG	0.654227	5.1:+5541836	None:intergenic
CTCTTCGACACCGACAACGA+CGG	0.661849	5.1:-5541886	MS.gene21155:CDS
TCGAGAGAAATCGAAAGGGG+AGG	0.668937	5.1:+5541765	None:intergenic
AAATCGAGAGAAATCGAAAG+GGG	0.678072	5.1:+5541762	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTTAATGATATTTAATAAA+AGG	+	chr5.1:5541189-5541208	None:intergenic	10.0%
!!	ATTAAAAAAAAAGTGATAAT+CGG	+	chr5.1:5541565-5541584	None:intergenic	10.0%
!!	AATAAAAAAAAGGAAAATCT+AGG	+	chr5.1:5541412-5541431	None:intergenic	15.0%
!!!	ATTTTTGACAAAATAATAGT+TGG	-	chr5.1:5541262-5541281	MS.gene21155:intron	15.0%
!!!	CATAAATATCAATTCTAAAA+TGG	-	chr5.1:5541708-5541727	MS.gene21155:CDS	15.0%
!!	TTTATTCGAGATAGAAAAAT+TGG	+	chr5.1:5541231-5541250	None:intergenic	20.0%
!!!	AGCTTTAAATTTTTGTTTCA+TGG	-	chr5.1:5541342-5541361	MS.gene21155:intron	20.0%
!!!	ATTTGTTTGTGTTTTATACT+TGG	-	chr5.1:5541442-5541461	MS.gene21155:intron	20.0%
!	AAAAAAGGAAAATCTAGGTT+AGG	+	chr5.1:5541407-5541426	None:intergenic	25.0%
!	AAAAAGGAAAATCTAGGTTA+GGG	+	chr5.1:5541406-5541425	None:intergenic	25.0%
!	AAATTATGGGACCAATTTAA+CGG	-	chr5.1:5541473-5541492	MS.gene21155:intron	25.0%
!	ACTCAAAATTATTGAGTATC+AGG	-	chr5.1:5541758-5541777	MS.gene21155:CDS	25.0%
!	CTATCTCGAATAAATAGTTA+AGG	-	chr5.1:5541237-5541256	MS.gene21155:intron	25.0%
!	CTCAAAATTATTGAGTATCA+GGG	-	chr5.1:5541759-5541778	MS.gene21155:CDS	25.0%
!	CTTGGTGAAATAGAAATTAT+GGG	-	chr5.1:5541460-5541479	MS.gene21155:intron	25.0%
!	TAAAGCTTAAAACCTTATCT+TGG	+	chr5.1:5541329-5541348	None:intergenic	25.0%
!	TCAAAATTATTGAGTATCAG+GGG	-	chr5.1:5541760-5541779	MS.gene21155:CDS	25.0%
!	TTAGTATGAAAGATGAAAGT+AGG	-	chr5.1:5541672-5541691	MS.gene21155:CDS	25.0%
!!	ACTTGGTGAAATAGAAATTA+TGG	-	chr5.1:5541459-5541478	MS.gene21155:intron	25.0%
!!!	AATATGCACCCTTTTAAAAA+GGG	-	chr5.1:5541504-5541523	MS.gene21155:intron	25.0%
!!!	CTCAATAATTTTGAGTTTGA+CGG	+	chr5.1:5541754-5541773	None:intergenic	25.0%
!!!	TAATATGCACCCTTTTAAAA+AGG	-	chr5.1:5541503-5541522	MS.gene21155:intron	25.0%
!!!	TTTTAAAAGGGTGCATATTA+GGG	+	chr5.1:5541503-5541522	None:intergenic	25.0%
!!!	TTTTTAAAAGGGTGCATATT+AGG	+	chr5.1:5541504-5541523	None:intergenic	25.0%
	AAAACACAAACAAATTGCAC+TGG	+	chr5.1:5541437-5541456	None:intergenic	30.0%
	AATCTGCATCGAGATAAAAT+CGG	+	chr5.1:5541649-5541668	None:intergenic	30.0%
	AATTATGGGACCAATTTAAC+GGG	-	chr5.1:5541474-5541493	MS.gene21155:intron	30.0%
	ATAAAATCGGGACAATATTG+TGG	+	chr5.1:5541636-5541655	None:intergenic	30.0%
	CAAAAATAATCACAGACCAA+TGG	-	chr5.1:5541833-5541852	MS.gene21155:CDS	30.0%
	GCATTCATCTTAACTATTTG+TGG	+	chr5.1:5541368-5541387	None:intergenic	30.0%
!	TGCACTGGTCAATAAAAAAA+AGG	+	chr5.1:5541422-5541441	None:intergenic	30.0%
!	TTTAAAAGGGTGCATATTAG+GGG	+	chr5.1:5541502-5541521	None:intergenic	30.0%
!!	AAGATGAAAGTAGGAGTATT+TGG	-	chr5.1:5541681-5541700	MS.gene21155:CDS	30.0%
!!	CATTGGTCTGTGATTATTTT+TGG	+	chr5.1:5541835-5541854	None:intergenic	30.0%
!!	TGAGTTTGATGAATGGATTA+GGG	-	chr5.1:5540970-5540989	MS.gene21155:intron	30.0%
!!	TTGGTGTTGCATTCAATTAT+CGG	-	chr5.1:5541281-5541300	MS.gene21155:intron	30.0%
	AAAAATGAATGGTCCATGCT+TGG	+	chr5.1:5541531-5541550	None:intergenic	35.0%
	AAATCGAGAGAAATCGAAAG+GGG	+	chr5.1:5540798-5540817	None:intergenic	35.0%
	AAATTATTGAGTATCAGGGG+TGG	-	chr5.1:5541763-5541782	MS.gene21155:CDS	35.0%
	AAGTACATGCAATGCAATGT+GGG	-	chr5.1:5541055-5541074	MS.gene21155:intron	35.0%
	AGAAATCGAGAGAAATCGAA+AGG	+	chr5.1:5540800-5540819	None:intergenic	35.0%
	ATCCATGTACATAACACCAT+TGG	+	chr5.1:5541852-5541871	None:intergenic	35.0%
	ATCTGCATCGAGATAAAATC+GGG	+	chr5.1:5541648-5541667	None:intergenic	35.0%
	CTACTGTGGGTAAAAATGAA+TGG	+	chr5.1:5541542-5541561	None:intergenic	35.0%
	GAAATCGAGAGAAATCGAAA+GGG	+	chr5.1:5540799-5540818	None:intergenic	35.0%
	GTTGCATTCAATTATCGGAA+CGG	-	chr5.1:5541286-5541305	MS.gene21155:intron	35.0%
	TCAGATTTCTCAACAGAAAC+AGG	-	chr5.1:5541890-5541909	MS.gene21155:CDS	35.0%
	TGAAGATTTCATCGCTAGAA+TGG	-	chr5.1:5541027-5541046	MS.gene21155:intron	35.0%
!	ATGCTTGGACCCTTTTTAAA+AGG	+	chr5.1:5541516-5541535	None:intergenic	35.0%
!	CTGAGTTTGATGAATGGATT+AGG	-	chr5.1:5540969-5540988	MS.gene21155:intron	35.0%
!	TGGAACAAGATGTTCCATTT+TGG	+	chr5.1:5541815-5541834	None:intergenic	35.0%
!!	AAGTTGATGTTGGGTTTGAT+GGG	-	chr5.1:5540993-5541012	MS.gene21155:intron	35.0%
!!	GGTGTTGTTTGAAACCAAAA+TGG	-	chr5.1:5541798-5541817	MS.gene21155:CDS	35.0%
!!	TGCTTGGACCCTTTTTAAAA+GGG	+	chr5.1:5541515-5541534	None:intergenic	35.0%
!!	TTTTAAAAAGGGTCCAAGCA+TGG	-	chr5.1:5541515-5541534	MS.gene21155:CDS	35.0%
!!!	CGTCATATTTTGACGAGTAT+TGG	-	chr5.1:5540929-5540948	MS.gene21155:intron	35.0%
	AAAGTGATAATCGGCTACTG+TGG	+	chr5.1:5541556-5541575	None:intergenic	40.0%
	AACCGTTCGATCATCAACTA+AGG	-	chr5.1:5540849-5540868	MS.gene21155:intron	40.0%
	AAGTGATAATCGGCTACTGT+GGG	+	chr5.1:5541555-5541574	None:intergenic	40.0%
	ACCGTTCGATCATCAACTAA+GGG	-	chr5.1:5540850-5540869	MS.gene21155:intron	40.0%
	ATCCATTCATCAAACTCAGC+AGG	+	chr5.1:5540968-5540987	None:intergenic	40.0%
	ATCTTGGACTAATTGACCCT+TGG	+	chr5.1:5541313-5541332	None:intergenic	40.0%
	ATTAGGGGAACCCGTTAAAT+TGG	+	chr5.1:5541487-5541506	None:intergenic	40.0%
	CAAGTACATGCAATGCAATG+TGG	-	chr5.1:5541054-5541073	MS.gene21155:intron	40.0%
	CACATTGCATTGCATGTACT+TGG	+	chr5.1:5541056-5541075	None:intergenic	40.0%
	GGGTCAATTAGTCCAAGATA+AGG	-	chr5.1:5541314-5541333	MS.gene21155:intron	40.0%
	GTTCTCGATAAAGATGCAAC+TGG	-	chr5.1:5540884-5540903	MS.gene21155:intron	40.0%
!!	GAAGTTGATGTTGGGTTTGA+TGG	-	chr5.1:5540992-5541011	MS.gene21155:intron	40.0%
!!	GACCAATGGTGTTATGTACA+TGG	-	chr5.1:5541847-5541866	MS.gene21155:CDS	40.0%
!!	GGATTAGGGAAGTTGATGTT+GGG	-	chr5.1:5540984-5541003	MS.gene21155:intron	40.0%
!!	TCCCTTAGTTGATGATCGAA+CGG	+	chr5.1:5540854-5540873	None:intergenic	40.0%
!!	TGGATTAGGGAAGTTGATGT+TGG	-	chr5.1:5540983-5541002	MS.gene21155:intron	40.0%
	AGCCTGCTGAGTTTGATGAA+TGG	-	chr5.1:5540963-5540982	MS.gene21155:intron	45.0%
	AGGGGAGGTTAGATTCTCTT+CGG	+	chr5.1:5540780-5540799	None:intergenic	45.0%
	CAATTATCGGAACGGTCCAA+GGG	-	chr5.1:5541294-5541313	MS.gene21155:intron	45.0%
	CTCAACAGAAACAGGACAAG+TGG	-	chr5.1:5541898-5541917	MS.gene21155:CDS	45.0%
	TCAATTATCGGAACGGTCCA+AGG	-	chr5.1:5541293-5541312	MS.gene21155:intron	45.0%
!	CGGTTCAGGTTTCATGTGTT+TGG	+	chr5.1:5540834-5540853	None:intergenic	45.0%
!	GATGGATTTGAGCTGAGCTT+GGG	+	chr5.1:5540753-5540772	None:intergenic	45.0%
!!	AGTTGATGATCGAACGGTTC+AGG	+	chr5.1:5540848-5540867	None:intergenic	45.0%
	ATTTGAGCTGAGCTTGGGTC+GGG	+	chr5.1:5540748-5540767	None:intergenic	50.0%
	GATTTGAGCTGAGCTTGGGT+CGG	+	chr5.1:5540749-5540768	None:intergenic	50.0%
	GGAATCCTCATGCGTTCTCT+CGG	-	chr5.1:5540716-5540735	MS.gene21155:intron	50.0%
	GTTAGGGAAGACATGACACG+AGG	+	chr5.1:5541390-5541409	None:intergenic	50.0%
	TAGATTCTCTTCGGCGACGA+TGG	+	chr5.1:5540771-5540790	None:intergenic	50.0%
	TCGAGAGAAATCGAAAGGGG+AGG	+	chr5.1:5540795-5540814	None:intergenic	50.0%
!	CGATGGATTTGAGCTGAGCT+TGG	+	chr5.1:5540754-5540773	None:intergenic	50.0%
	AAGATCGCACCATCGGAGCT+CGG	-	chr5.1:5540695-5540714	MS.gene21155:intron	55.0%
	ATCCTCATGCGTTCTCTCGG+CGG	-	chr5.1:5540719-5540738	MS.gene21155:intron	55.0%
	ATGAGGATTCCGAGCTCCGA+TGG	+	chr5.1:5540707-5540726	None:intergenic	55.0%
	CTCTTCGACACCGACAACGA+CGG	-	chr5.1:5540671-5540690	MS.gene21155:intron	55.0%
	TGCGATCTTGCCGTCGTTGT+CGG	+	chr5.1:5540684-5540703	None:intergenic	55.0%
	CAGGGGTGGCACTCGAGAAT+CGG	-	chr5.1:5541777-5541796	MS.gene21155:CDS	60.0%
	CGACGGCAAGATCGCACCAT+CGG	-	chr5.1:5540688-5540707	MS.gene21155:intron	60.0%
	TACCGCCGAGAGAACGCATG+AGG	+	chr5.1:5540724-5540743	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	5540653	5541926	5540653	ID=MS.gene21155
chr5.1	mRNA	5540653	5541926	5540653	ID=MS.gene21155.t1;Parent=MS.gene21155
chr5.1	exon	5541507	5541926	5541507	ID=MS.gene21155.t1.exon1;Parent=MS.gene21155.t1
chr5.1	CDS	5541507	5541926	5541507	ID=cds.MS.gene21155.t1;Parent=MS.gene21155.t1
chr5.1	exon	5540653	5540685	5540653	ID=MS.gene21155.t1.exon2;Parent=MS.gene21155.t1
chr5.1	CDS	5540653	5540685	5540653	ID=cds.MS.gene21155.t1;Parent=MS.gene21155.t1

Gene Sequence

>MS.gene21155

ATGAAAGAAGCATTCACACTCTTCGACACCGACAACGACGGCAAGATCGCACCATCGGAGCTCGGAATCCTCATGCGTTCTCTCGGCGGTAACCCGACCCAAGCTCAGCTCAAATCCATCGTCGCCGAAGAGAATCTAACCTCCCCTTTCGATTTCTCTCGATTTCTCGATCTCATGTCCAAACACATGAAACCTGAACCGTTCGATCATCAACTAAGGGATGCGTTCAAAGTTCTCGATAAAGATGCAACTGGATTTGTTTCTGTGAATGAGCTTCGTCATATTTTGACGAGTATTGGTGAGAAGCTTGAGCCTGCTGAGTTTGATGAATGGATTAGGGAAGTTGATGTTGGGTTTGATGGGAAGATTCGTTATGAAGATTTCATCGCTAGAATGGTCGCCAAGTACATGCAATGCAATGTGGGAATGCTTTTTTATTTCTCTAAAATTATATTTTGTTTCTTTTTAACTGTCGTTTTTAGTTTATCTAAACATATACAAAGATCTAATAATATTTTTTTTGTTATTATTTGCCTTTTATTAAATATCATTAACTATATATTTTCTTATTTTTACCAATTTTTCTATCTCGAATAAATAGTTAAGGAGATTTTTGACAAAATAATAGTTGGTGTTGCATTCAATTATCGGAACGGTCCAAGGGTCAATTAGTCCAAGATAAGGTTTTAAGCTTTAAATTTTTGTTTCATGGCCACAAATAGTTAAGATGAATGCCTCGTGTCATGTCTTCCCTAACCTAGATTTTCCTTTTTTTTATTGACCAGTGCAATTTGTTTGTGTTTTATACTTGGTGAAATAGAAATTATGGGACCAATTTAACGGGTTCCCCTAATATGCACCCTTTTAAAAAGGGTCCAAGCATGGACCATTCATTTTTACCCACAGTAGCCGATTATCACTTTTTTTTTAATAATTGTTTTAAGTTCATTTTTTATTAAATACATCTCTCTCTCTCTCAACCACAATATTGTCCCGATTTTATCTCGATGCAGATTATTTTAGTATGAAAGATGAAAGTAGGAGTATTTGGTTATCATAAATATCAATTCTAAAATGGATTGTCAAATTTTTTGTTTACCGTCAAACTCAAAATTATTGAGTATCAGGGGTGGCACTCGAGAATCGGTGTTGTTTGAAACCAAAATGGAACATCTTGTTCCAAAAATAATCACAGACCAATGGTGTTATGTACATGGATAAAAGAACTGATTTTGTTTCAGATTTCTCAACAGAAACAGGACAAGTGGAAGTGA

Protein sequence

>MS.gene21155.t1

MKEAFTLFDTDNDGKIAPSELGILMRSLGGNPTQAQLKSIVAEENLTSPFDFSRFLDLMSKHMKPEPFDHQLRDAFKVLDKDATGFVSVNELRHILTSIGEKLEPAEFDEWIREVDVGFDGKIRYEDFIARMVAKYMQCNISQQKQDKWK