AlfalfaGEDB | MS.gene89216

M. sativa cultivar XinJiangDaYe / MS.gene89216

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89216.t1	XP_003616365.1	98.6	138	2	0	1	138	1	138	4.00E-28	134.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89216.t1	Q9C8Y1	65.9	138	44	1	3	137	14	151	7.6e-12	71.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89216.t1	G7K1X5	98.6	138	2	0	1	138	1	138	2.9e-28	134.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049983	MS.gene89216	0.803034	4.23E-49	-1.69E-46
MS.gene052016	MS.gene89216	0.847632	1.02E-59	-1.69E-46
MS.gene052493	MS.gene89216	0.801512	8.69E-49	-1.69E-46
MS.gene054892	MS.gene89216	0.823936	1.06E-53	-1.69E-46
MS.gene054893	MS.gene89216	0.820249	7.58E-53	-1.69E-46
MS.gene057155	MS.gene89216	0.804542	2.06E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene000724	MS.gene89216	PPI
MS.gene000738	MS.gene89216	PPI
MS.gene001246	MS.gene89216	PPI
MS.gene001381	MS.gene89216	PPI
MS.gene003880	MS.gene89216	PPI
MS.gene004899	MS.gene89216	PPI
MS.gene008225	MS.gene89216	PPI
MS.gene008831	MS.gene89216	PPI
MS.gene009021	MS.gene89216	PPI
MS.gene011400	MS.gene89216	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89216.t1	MTR_5g079340	95.683	139	6	0	1	139	1	139	1.51e-93	266
MS.gene89216.t1	MTR_3g067610	74.453	137	32	1	3	139	4	137	8.58e-71	209
MS.gene89216.t1	MTR_1g047100	74.648	142	30	2	1	138	1	140	2.02e-69	206
MS.gene89216.t1	MTR_1g046950	68.750	144	38	2	1	139	1	142	7.91e-66	196
MS.gene89216.t1	MTR_5g008705	70.000	130	34	2	12	138	2	129	5.28e-56	171
MS.gene89216.t1	MTR_5g008695	70.085	117	28	2	28	139	1	115	9.16e-50	155
MS.gene89216.t1	MTR_8g107110	58.519	135	56	0	3	137	23	157	1.58e-49	156
MS.gene89216.t1	MTR_6g079570	56.204	137	59	1	3	138	25	161	2.99e-46	148
MS.gene89216.t1	MTR_1g032070	50.735	136	66	1	3	137	45	180	1.84e-41	137
MS.gene89216.t1	MTR_8g105230	47.727	132	68	1	4	134	36	167	1.92e-38	129

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89216.t1	AT5G37770	55.319	141	58	1	3	138	16	156	3.59e-49	155
MS.gene89216.t1	AT5G37770	55.319	141	58	1	3	138	16	156	3.59e-49	155
MS.gene89216.t1	AT1G66400	55.797	138	58	2	3	137	14	151	1.23e-48	154
MS.gene89216.t1	AT1G18210	54.815	135	58	2	3	137	22	153	3.00e-47	150
MS.gene89216.t1	AT1G18210	54.815	135	58	2	3	137	22	153	3.00e-47	150
MS.gene89216.t1	AT1G73630	53.285	137	61	2	2	138	18	151	4.57e-46	147
MS.gene89216.t1	AT1G24620	49.265	136	68	1	4	138	37	172	8.21e-42	137
MS.gene89216.t1	AT2G15680	48.529	136	69	1	3	138	49	183	8.61e-40	132
MS.gene89216.t1	AT5G17470	46.763	139	67	2	5	138	3	139	7.62e-36	120
MS.gene89216.t1	AT2G36180	48.889	135	65	1	7	137	3	137	2.20e-34	117

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Find 13 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGATGCTGTTAACACT+CGG	0.667064	5.2:-67505510	MS.gene89216:CDS
AATGTTGATGTTGACGGTGA+TGG	0.518258	5.2:-67505231	MS.gene89216:CDS
AGAAGAAGTGAAACGCATGA+TGG	0.605973	5.2:-67505472	MS.gene89216:CDS
AGAAGTGAAACGCATGATGG+AGG	0.624498	5.2:-67505469	MS.gene89216:CDS
AGGTTCGGTGCAGTGAAAAT+CGG	0.467777	5.2:+67505401	None:intergenic
ATCAAAAGCATCACGAAGCT+CGG	0.520647	5.2:+67505362	None:intergenic
ATGGAGGAGCTCGATCAAAA+CGG	0.537572	5.2:-67505453	MS.gene89216:CDS
ATTAGTAATGTTGATGTTGA+CGG	0.468849	5.2:-67505237	MS.gene89216:CDS
CGAAGAGTTCAAGAAGATGA+TGG	0.555496	5.2:-67505196	MS.gene89216:CDS
CTCTACGATCTTGATAAGAA+CGG	0.590565	5.2:-67505339	MS.gene89216:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAGATGCTGTTAACACT+CGG	-	chr5.2:67505263-67505282	MS.gene89216:CDS	35.0%
CTCTACGATCTTGATAAGAA+CGG	-	chr5.2:67505434-67505453	MS.gene89216:CDS	35.0%
TTCAGCAAGTTCGACAAAAA+TGG	-	chr5.2:67505212-67505231	MS.gene89216:CDS	35.0%
AAGTTCGACAAAAATGGCGA+TGG	-	chr5.2:67505218-67505237	MS.gene89216:CDS	40.0%
AATGTTGATGTTGACGGTGA+TGG	-	chr5.2:67505542-67505561	MS.gene89216:CDS	40.0%
AGAAGAAGTGAAACGCATGA+TGG	-	chr5.2:67505301-67505320	MS.gene89216:CDS	40.0%
CGAAGAGTTCAAGAAGATGA+TGG	-	chr5.2:67505577-67505596	MS.gene89216:CDS	40.0%
AGAAGTGAAACGCATGATGG+AGG	-	chr5.2:67505304-67505323	MS.gene89216:CDS	45.0%
ATGGAGGAGCTCGATCAAAA+CGG	-	chr5.2:67505320-67505339	MS.gene89216:CDS	45.0%
CTGCACCGAACCTGGAAAAG+AGG	-	chr5.2:67505382-67505401	MS.gene89216:CDS	55.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	67505188	67505607	67505188	ID=MS.gene89216
chr5.2	mRNA	67505188	67505607	67505188	ID=MS.gene89216.t1;Parent=MS.gene89216
chr5.2	exon	67505188	67505607	67505188	ID=MS.gene89216.t1.exon1;Parent=MS.gene89216.t1
chr5.2	CDS	67505188	67505607	67505188	ID=cds.MS.gene89216.t1;Parent=MS.gene89216.t1

Gene Sequence

>MS.gene89216

ATGGATGATGAAGTGCGCAAGATCTTCAGCAAGTTCGACAAAAATGGCGATGGAAAAATCTCACGTTCAGAACTCAAAGAGATGCTGTTAACACTCGGATCAGAAACTACATCAGAAGAAGTGAAACGCATGATGGAGGAGCTCGATCAAAACGGCGACGGTTTCATTGATCTGAAAGAATTCACCGATTTTCACTGCACCGAACCTGGAAAAGAGGATTCTACCGAGCTTCGTGATGCTTTTGATCTCTACGATCTTGATAAGAACGGTTTGATCTCTGCTAATGAATTGCACGCTGTTTTGAAGAAACTCGGTGAAAAATGCTCGTTGAATGATTGTAAGAAGATGATTAGTAATGTTGATGTTGACGGTGATGGAAATGTTAACTTCGAAGAGTTCAAGAAGATGATGGCTCGCTGA

Protein sequence

>MS.gene89216.t1

MDDEVRKIFSKFDKNGDGKISRSELKEMLLTLGSETTSEEVKRMMEELDQNGDGFIDLKEFTDFHCTEPGKEDSTELRDAFDLYDLDKNGLISANELHAVLKKLGEKCSLNDCKKMISNVDVDGDGNVNFEEFKKMMAR