AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65954


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65954.t1 MTR_1g030440 93.548 155 7 2 1 154 15 167 2.09e-97 278
MS.gene65954.t1 MTR_3g088655 64.968 157 48 3 1 150 1 157 2.81e-57 176
MS.gene65954.t1 MTR_7g074240 35.333 150 88 3 9 150 25 173 2.73e-20 82.8
MS.gene65954.t1 MTR_3g067610 34.000 150 78 3 4 147 1 135 9.83e-19 77.4
MS.gene65954.t1 MTR_4g067270 36.913 149 75 4 7 147 4 141 1.75e-18 77.0
MS.gene65954.t1 MTR_8g105230 38.462 143 71 4 8 144 36 167 3.49e-18 77.4
MS.gene65954.t1 MTR_1g046950 36.735 147 78 3 7 147 3 140 1.19e-17 74.7
MS.gene65954.t1 MTR_5g079340 35.374 147 77 3 7 147 3 137 4.28e-17 73.2
MS.gene65954.t1 MTR_6g023460 36.364 143 73 3 12 148 12 142 3.14e-16 71.2
MS.gene65954.t1 MTR_1g032070 34.014 147 80 3 8 148 46 181 8.87e-16 71.2
MS.gene65954.t1 MTR_1g047100 33.333 147 82 2 7 147 3 139 2.69e-15 68.6
MS.gene65954.t1 MTR_6g079570 35.065 154 79 5 7 152 25 165 8.79e-15 68.2
MS.gene65954.t1 MTR_8g066630 32.432 148 84 3 7 147 16 154 9.22e-15 67.8
MS.gene65954.t1 MTR_5g092810 32.450 151 83 4 9 153 398 535 1.40e-14 70.1
MS.gene65954.t1 MTR_4g112460 30.612 147 87 3 7 147 10 147 2.55e-14 66.6
MS.gene65954.t1 MTR_8g107110 31.206 141 91 1 7 147 23 157 2.59e-14 66.6
MS.gene65954.t1 MTR_7g089760 34.211 152 81 3 4 147 1 141 2.68e-14 66.2
MS.gene65954.t1 MTR_2g098890 35.570 149 79 3 7 147 63 202 4.10e-14 67.0
MS.gene65954.t1 MTR_4g086260 29.730 148 97 2 7 154 56 196 4.86e-14 66.6
MS.gene65954.t1 MTR_4g132070 32.414 145 79 4 9 147 397 528 5.47e-14 68.6
MS.gene65954.t1 MTR_0028s0170 31.034 145 81 4 9 147 384 515 7.29e-14 68.2
MS.gene65954.t1 MTR_7g075040 36.424 151 80 6 7 147 46 190 1.78e-13 65.1
MS.gene65954.t1 MTR_8g099095 32.414 145 79 4 9 147 372 503 2.48e-13 66.6
MS.gene65954.t1 MTR_8g036075 32.468 154 89 5 8 152 49 196 9.41e-13 63.2
MS.gene65954.t1 MTR_3g051770 31.126 151 80 4 9 147 389 527 1.10e-12 64.7
MS.gene65954.t1 MTR_1g054865 30.986 142 84 3 9 147 467 597 1.51e-12 64.3
MS.gene65954.t1 MTR_5g009830 33.103 145 78 4 9 147 372 503 2.11e-12 63.9
MS.gene65954.t1 MTR_5g089320 30.345 145 82 4 9 147 374 505 2.90e-12 63.5
MS.gene65954.t1 MTR_3g083470 32.168 143 82 4 7 149 92 219 3.22e-12 62.4
MS.gene65954.t1 MTR_4g115170 33.121 157 88 3 6 147 62 216 3.97e-12 62.0
MS.gene65954.t1 MTR_5g017550 30.872 149 85 5 6 148 46 182 4.52e-12 61.2
MS.gene65954.t1 MTR_4g103650 31.429 140 90 1 8 147 2 135 7.07e-12 59.7
MS.gene65954.t1 MTR_5g017510 32.414 145 89 4 6 148 46 183 7.94e-12 60.5
MS.gene65954.t1 MTR_5g017560 32.414 145 89 4 6 148 46 183 7.94e-12 60.5
MS.gene65954.t1 MTR_5g088320 29.333 150 85 5 7 148 11 147 8.07e-12 59.7
MS.gene65954.t1 MTR_6g025320 29.333 150 85 5 7 148 11 147 8.24e-12 59.7
MS.gene65954.t1 MTR_1g041285 30.872 149 82 4 8 148 12 147 1.39e-11 59.3
MS.gene65954.t1 MTR_8g069915 30.714 140 94 2 8 147 12 148 1.40e-11 59.7
MS.gene65954.t1 MTR_7g087610 29.333 150 85 5 7 148 11 147 1.81e-11 58.9
MS.gene65954.t1 MTR_1g076650 29.577 142 83 3 13 148 17 147 1.92e-11 58.9
MS.gene65954.t1 MTR_4g103655 31.429 140 90 1 8 147 6 139 2.54e-11 58.2
MS.gene65954.t1 MTR_1g026190 29.496 139 91 2 9 147 388 519 4.52e-11 60.1
MS.gene65954.t1 MTR_4g132040 33.103 145 78 4 9 147 401 532 4.90e-11 60.1
MS.gene65954.t1 MTR_3g098090 30.935 139 88 2 9 147 338 468 5.94e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65954.t1 AT1G21550 46.358 151 72 5 4 148 6 153 1.32e-31 111
MS.gene65954.t1 AT4G20780 40.909 154 76 4 9 147 30 183 1.97e-25 96.3
MS.gene65954.t1 AT5G44460 39.456 147 80 3 9 147 29 174 2.72e-20 82.8
MS.gene65954.t1 AT1G24620 36.735 147 86 2 8 153 37 177 1.42e-19 80.9
MS.gene65954.t1 AT2G43290 35.570 149 82 3 7 147 64 206 1.13e-18 79.3
MS.gene65954.t1 AT5G37770 31.579 152 91 2 2 147 11 155 2.42e-16 72.0
MS.gene65954.t1 AT5G37770 31.579 152 91 2 2 147 11 155 2.42e-16 72.0
MS.gene65954.t1 AT4G04710 34.483 145 76 4 9 147 338 469 6.19e-16 73.9
MS.gene65954.t1 AT4G04710 34.483 145 76 4 9 147 324 455 7.42e-16 73.9
MS.gene65954.t1 AT4G04710 34.483 145 76 4 9 147 351 482 8.36e-16 73.9
MS.gene65954.t1 AT1G05990 34.228 149 80 3 7 147 4 142 9.51e-16 70.1
MS.gene65954.t1 AT1G73630 32.414 145 89 1 3 147 15 150 1.03e-15 70.5
MS.gene65954.t1 AT4G04710 34.014 147 76 4 9 147 351 484 1.54e-15 73.2
MS.gene65954.t1 AT5G12180 31.724 145 80 3 9 147 379 510 1.97e-15 72.8
MS.gene65954.t1 AT5G19360 31.034 145 81 3 9 147 374 505 3.09e-15 72.0
MS.gene65954.t1 AT1G76640 34.247 146 81 2 8 147 22 158 3.26e-15 68.9
MS.gene65954.t1 AT4G23650 35.862 145 74 4 9 147 384 515 4.18e-15 71.6
MS.gene65954.t1 AT1G32250 32.653 147 88 2 7 147 15 156 5.74e-15 68.6
MS.gene65954.t1 AT4G12860 32.237 152 84 3 4 147 1 141 5.85e-15 68.2
MS.gene65954.t1 AT3G59440 33.784 148 78 3 8 147 51 186 8.84e-15 68.6
MS.gene65954.t1 AT1G66400 29.677 155 94 2 2 150 9 154 9.11e-15 67.8
MS.gene65954.t1 AT1G18530 34.028 144 84 3 8 147 7 143 3.60e-14 66.2
MS.gene65954.t1 AT5G42380 35.417 144 89 1 4 147 45 184 8.89e-14 65.9
MS.gene65954.t1 AT4G04695 32.414 145 79 4 9 147 337 468 1.10e-13 67.8
MS.gene65954.t1 AT4G03290 32.215 149 85 3 7 147 4 144 1.13e-13 64.7
MS.gene65954.t1 AT4G04700 33.103 145 78 4 9 147 337 468 1.68e-13 67.0
MS.gene65954.t1 AT3G07490 33.553 152 82 3 4 147 1 141 1.98e-13 64.3
MS.gene65954.t1 AT5G17470 31.690 142 82 4 12 147 6 138 5.73e-13 62.8
MS.gene65954.t1 AT1G76650 34.932 146 77 3 8 147 43 176 1.30e-12 62.4
MS.gene65954.t1 AT1G76650 34.932 146 77 3 8 147 43 176 1.30e-12 62.4
MS.gene65954.t1 AT4G04720 31.206 141 86 3 9 147 385 516 1.47e-12 64.3
MS.gene65954.t1 AT4G04720 31.206 141 86 3 9 147 385 516 1.47e-12 64.3
MS.gene65954.t1 AT1G50700 29.655 145 83 4 9 147 379 510 1.55e-12 64.3
MS.gene65954.t1 AT2G41100 29.371 143 88 3 13 143 17 158 1.92e-12 63.9
MS.gene65954.t1 AT2G41100 29.371 143 88 3 13 143 17 158 1.92e-12 63.9
MS.gene65954.t1 AT1G35670 33.103 145 77 4 9 147 332 462 2.50e-12 63.9
MS.gene65954.t1 AT2G15680 30.496 141 91 1 7 147 49 182 2.58e-12 62.0
MS.gene65954.t1 AT4G09570 33.099 142 81 3 9 147 331 461 4.78e-12 62.8
MS.gene65954.t1 AT1G18210 30.000 140 89 1 8 147 23 153 5.30e-12 60.8
MS.gene65954.t1 AT1G18210 30.000 140 89 1 8 147 23 153 5.30e-12 60.8
MS.gene65954.t1 AT4G04740 31.206 141 86 3 9 147 374 505 5.30e-12 62.8
MS.gene65954.t1 AT4G04740 31.206 141 86 3 9 147 374 505 5.30e-12 62.8
MS.gene65954.t1 AT4G04740 31.206 141 86 3 9 147 374 505 5.30e-12 62.8
MS.gene65954.t1 AT4G04740 31.206 141 86 3 9 147 374 505 5.30e-12 62.8
MS.gene65954.t1 AT4G04740 31.206 141 86 3 9 147 374 505 5.30e-12 62.8
MS.gene65954.t1 AT4G04740 31.206 141 86 3 9 147 374 505 5.30e-12 62.8
MS.gene65954.t1 AT3G43810 29.333 150 85 5 7 148 11 147 8.63e-12 59.7
MS.gene65954.t1 AT4G21940 31.724 145 80 4 9 147 407 538 1.14e-11 62.0
MS.gene65954.t1 AT3G56800 29.333 150 85 5 7 148 11 147 1.16e-11 59.3
MS.gene65954.t1 AT2G27030 29.333 150 85 5 7 148 11 147 1.16e-11 59.3
MS.gene65954.t1 AT2G41110 29.333 150 85 5 7 148 11 147 1.16e-11 59.3
MS.gene65954.t1 AT5G21274 29.333 150 85 5 7 148 11 147 1.18e-11 59.3
MS.gene65954.t1 AT4G21940 31.724 145 80 4 9 147 407 538 1.21e-11 62.0
MS.gene65954.t1 AT2G27030 29.333 150 85 5 7 148 11 147 1.46e-11 59.7
MS.gene65954.t1 AT3G20410 30.345 145 82 4 9 147 397 528 1.48e-11 61.6
MS.gene65954.t1 AT3G20410 30.345 145 82 4 9 147 397 528 1.48e-11 61.6
MS.gene65954.t1 AT3G43810 29.333 150 85 5 7 148 11 147 1.72e-11 60.1
MS.gene65954.t1 AT1G50700 29.560 159 91 5 9 147 379 536 1.84e-11 61.2
MS.gene65954.t1 AT3G51850 30.822 146 83 3 8 147 359 492 3.15e-11 60.5
MS.gene65954.t1 AT5G37780 26.351 148 92 2 7 148 11 147 3.59e-11 58.2
MS.gene65954.t1 AT1G66410 26.351 148 92 2 7 148 11 147 3.59e-11 58.2
MS.gene65954.t1 AT1G76650 34.286 140 76 3 8 147 43 166 5.41e-11 58.2
MS.gene65954.t1 AT2G36180 32.168 143 81 4 12 147 4 137 8.39e-11 57.0
MS.gene65954.t1 AT1G61950 30.345 145 82 4 9 147 405 536 9.70e-11 59.3

Find 25 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATATGATATTAATTTAGA+TGG 0.283864 1.2:-15971057 MS.gene65954:CDS
TTCAAGGTGTTTGATTTAGA+TGG 0.287480 1.2:-15971171 MS.gene65954:CDS
TCCCCTAACCCGCAATGAAT+TGG 0.338232 1.2:-15971436 MS.gene65954:CDS
CTCCAATTCATTGCGGGTTA+GGG 0.340726 1.2:+15971434 None:intergenic
GCTCCAATTCATTGCGGGTT+AGG 0.348062 1.2:+15971433 None:intergenic
AATCAATTGTTAGTGAGAAC+AGG 0.403944 1.2:-15971354 MS.gene65954:CDS
AGATGGCAAGCTTGATTTCA+AGG 0.404957 1.2:-15971040 MS.gene65954:CDS
TATCAGCTCCAATTCATTGC+GGG 0.425822 1.2:+15971428 None:intergenic
GTGTTTGATTTAGATGGAGA+TGG 0.487484 1.2:-15971165 MS.gene65954:CDS
TGGCATGTGGGATGAAGAAA+AGG 0.494222 1.2:-15971100 MS.gene65954:CDS
ACACCTTGAAAGCCTTCAAA+AGG 0.524154 1.2:+15971184 None:intergenic
ATTCATCACTAGTCAAGAGC+TGG 0.543089 1.2:-15971142 MS.gene65954:CDS
ACAATTGATTCAGCTCCTCA+AGG 0.548233 1.2:+15971367 None:intergenic
AAACTAGACATAAATAGTGA+TGG 0.557379 1.2:-15971399 MS.gene65954:CDS
TCCAATTCATTGCGGGTTAG+GGG 0.573279 1.2:+15971435 None:intergenic
TGATGGTGGTGATGAGATTG+AGG 0.579256 1.2:-15971226 MS.gene65954:CDS
CAATTGATTCAGCTCCTCAA+GGG 0.587040 1.2:+15971368 None:intergenic
AGAGGAGCTTGAATATCTAG+TGG 0.598842 1.2:-15971316 MS.gene65954:CDS
AGGTTTCAAGTATAGCATAG+AGG 0.617626 1.2:-15971334 MS.gene65954:CDS
GAGGAGCTTGAATATCTAGT+GGG 0.620423 1.2:-15971315 MS.gene65954:CDS
GAATGCGTGTTGAAGAGACT+TGG 0.646626 1.2:-15971120 MS.gene65954:CDS
TGTTGAAGAGACTTGGCATG+TGG 0.651973 1.2:-15971113 MS.gene65954:CDS
ATATCAGCTCCAATTCATTG+CGG 0.652735 1.2:+15971427 None:intergenic
GTTGAAGAGACTTGGCATGT+GGG 0.658068 1.2:-15971112 MS.gene65954:CDS
TGGTGGTGATGAGATTGAGG+AGG 0.715134 1.2:-15971223 MS.gene65954:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATATGATATTAATTTAGA+TGG - chr1.2:15971383-15971402 MS.gene65954:CDS 15.0%
!! TCAATATTGAATCACAAAAA+TGG - chr1.2:15971191-15971210 MS.gene65954:CDS 20.0%
! AAACTAGACATAAATAGTGA+TGG - chr1.2:15971041-15971060 MS.gene65954:CDS 25.0%
AATCAATTGTTAGTGAGAAC+AGG - chr1.2:15971086-15971105 MS.gene65954:CDS 30.0%
TTGAATCACAAAAATGGTGA+TGG - chr1.2:15971197-15971216 MS.gene65954:CDS 30.0%
! ATTAGAAAGTGACCTTTTGA+AGG - chr1.2:15971244-15971263 MS.gene65954:CDS 30.0%
! TTCAAGGTGTTTGATTTAGA+TGG - chr1.2:15971269-15971288 MS.gene65954:CDS 30.0%
ATATCAGCTCCAATTCATTG+CGG + chr1.2:15971016-15971035 None:intergenic 35.0%
! AGGTTTCAAGTATAGCATAG+AGG - chr1.2:15971106-15971125 MS.gene65954:CDS 35.0%
! GTGTTTGATTTAGATGGAGA+TGG - chr1.2:15971275-15971294 MS.gene65954:CDS 35.0%
! TGATGGAATTTTATCCCTTG+AGG - chr1.2:15971058-15971077 MS.gene65954:CDS 35.0%
!! AATCACAAAAATGGTGATGG+TGG - chr1.2:15971200-15971219 MS.gene65954:CDS 35.0%
ACACCTTGAAAGCCTTCAAA+AGG + chr1.2:15971259-15971278 None:intergenic 40.0%
AGAGGAGCTTGAATATCTAG+TGG - chr1.2:15971124-15971143 MS.gene65954:CDS 40.0%
AGATGGCAAGCTTGATTTCA+AGG - chr1.2:15971400-15971419 MS.gene65954:CDS 40.0%
ATTCATCACTAGTCAAGAGC+TGG - chr1.2:15971298-15971317 MS.gene65954:CDS 40.0%
GAGGAGCTTGAATATCTAGT+GGG - chr1.2:15971125-15971144 MS.gene65954:CDS 40.0%
TATCAGCTCCAATTCATTGC+GGG + chr1.2:15971015-15971034 None:intergenic 40.0%
! ACAATTGATTCAGCTCCTCA+AGG + chr1.2:15971076-15971095 None:intergenic 40.0%
! CAATTGATTCAGCTCCTCAA+GGG + chr1.2:15971075-15971094 None:intergenic 40.0%
!! TGACCTTTTGAAGGCTTTCA+AGG - chr1.2:15971253-15971272 MS.gene65954:CDS 40.0%
GAATGCGTGTTGAAGAGACT+TGG - chr1.2:15971320-15971339 MS.gene65954:CDS 45.0%
GTTGAAGAGACTTGGCATGT+GGG - chr1.2:15971328-15971347 MS.gene65954:CDS 45.0%
TGGCATGTGGGATGAAGAAA+AGG - chr1.2:15971340-15971359 MS.gene65954:CDS 45.0%
TGTTGAAGAGACTTGGCATG+TGG - chr1.2:15971327-15971346 MS.gene65954:CDS 45.0%
! CTCCAATTCATTGCGGGTTA+GGG + chr1.2:15971009-15971028 None:intergenic 45.0%
!! TCCAATTCATTGCGGGTTAG+GGG + chr1.2:15971008-15971027 None:intergenic 45.0%
!! TGATGGTGGTGATGAGATTG+AGG - chr1.2:15971214-15971233 MS.gene65954:CDS 45.0%
GCTCCAATTCATTGCGGGTT+AGG + chr1.2:15971010-15971029 None:intergenic 50.0%
TCCCCTAACCCGCAATGAAT+TGG - chr1.2:15971004-15971023 MS.gene65954:CDS 50.0%
! TGGTGGTGATGAGATTGAGG+AGG - chr1.2:15971217-15971236 MS.gene65954:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 15970999 15971463 15970999 ID=MS.gene65954
chr1.2 mRNA 15970999 15971463 15970999 ID=MS.gene65954.t1;Parent=MS.gene65954
chr1.2 exon 15970999 15971463 15970999 ID=MS.gene65954.t1.exon1;Parent=MS.gene65954.t1
chr1.2 CDS 15970999 15971463 15970999 ID=cds.MS.gene65954.t1;Parent=MS.gene65954.t1
Gene Sequence

>MS.gene65954

ATGTGTCCCCTAACCCGCAATGAATTGGAGCTGATATTTAAGAAACTAGACATAAATAGTGATGGAATTTTATCCCTTGAGGAGCTGAATCAATTGTTAGTGAGAACAGGTTTCAAGTATAGCATAGAGGAGCTTGAATATCTAGTGGGAAAGAAGAGTCTAGACTTGAGTGAGTTCTTGTGCTTTTATGACTCAATATTGAATCACAAAAATGGTGATGGTGGTGATGAGATTGAGGAGGTAGAATTAGAAAGTGACCTTTTGAAGGCTTTCAAGGTGTTTGATTTAGATGGAGATGGATTCATCACTAGTCAAGAGCTGGAATGCGTGTTGAAGAGACTTGGCATGTGGGATGAAGAAAAGGATTCAAGATCAATGATTTACTCATATGATATTAATTTAGATGGCAAGCTTGATTTCAAGGAATTTAAGAACATGATGTTGCTTACACTACAACGTGTTTAG

Protein sequence

>MS.gene65954.t1

MCPLTRNELELIFKKLDINSDGILSLEELNQLLVRTGFKYSIEELEYLVGKKSLDLSEFLCFYDSILNHKNGDGGDEIEEVELESDLLKAFKVFDLDGDGFITSQELECVLKRLGMWDEEKDSRSMIYSYDINLDGKLDFKEFKNMMLLTLQRV