AlfalfaGEDB | MS.gene64324

M. sativa cultivar XinJiangDaYe / MS.gene64324

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64324.t1	XP_013467196.1	68.3	142	42	1	1	139	1	142	5.90E-40	173.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64324.t1	Q9M7R0	48.2	139	68	1	3	137	19	157	2.4e-29	129.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64324.t1	A0A072VHE0	68.3	142	42	1	1	139	1	142	4.3e-40	173.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene64324	MS.gene64327	PPI
MS.gene64324	MS.gene62258	PPI
MS.gene64324	MS.gene28338	PPI
MS.gene64324	MS.gene041795	PPI
MS.gene64324	MS.gene55496	PPI
MS.gene64324	MS.gene025797	PPI
MS.gene64324	MS.gene21931	PPI
MS.gene64324	MS.gene001381	PPI
MS.gene64324	MS.gene87673	PPI
MS.gene64324	MS.gene27516	PPI
MS.gene64324	MS.gene040127	PPI
MS.gene64324	MS.gene53659	PPI
MS.gene64324	MS.gene09960	PPI
MS.gene64324	MS.gene44354	PPI
MS.gene64324	MS.gene011400	PPI
MS.gene64324	MS.gene061768	PPI
MS.gene64324	MS.gene62540	PPI
MS.gene64324	MS.gene47134	PPI
MS.gene64324	MS.gene017446	PPI
MS.gene64324	MS.gene80714	PPI
MS.gene64324	MS.gene96914	PPI
MS.gene64324	MS.gene02545	PPI
MS.gene64324	MS.gene96913	PPI
MS.gene64324	MS.gene61009	PPI
MS.gene64324	MS.gene35563	PPI
MS.gene64324	MS.gene057167	PPI
MS.gene038456	MS.gene64324	PPI
MS.gene64324	MS.gene93763	PPI
MS.gene64324	MS.gene64313	PPI
MS.gene64324	MS.gene49270	PPI
MS.gene004899	MS.gene64324	PPI
MS.gene64324	MS.gene64307	PPI
MS.gene64324	MS.gene027655	PPI
MS.gene64324	MS.gene024717	PPI
MS.gene64324	MS.gene036930	PPI
MS.gene64324	MS.gene21932	PPI
MS.gene64324	MS.gene026618	PPI
MS.gene64324	MS.gene58860	PPI
MS.gene64324	MS.gene29794	PPI
MS.gene64324	MS.gene065998	PPI
MS.gene64324	MS.gene53648	PPI
MS.gene64324	MS.gene008831	PPI
MS.gene64324	MS.gene71511	PPI
MS.gene64324	MS.gene033361	PPI
MS.gene64324	MS.gene056461	PPI
MS.gene64324	MS.gene036602	PPI
MS.gene64324	MS.gene89216	PPI
MS.gene64324	MS.gene35562	PPI
MS.gene64324	MS.gene64308	PPI
MS.gene64324	MS.gene009021	PPI
MS.gene64324	MS.gene47195	PPI
MS.gene68345	MS.gene64324	PPI
MS.gene64324	MS.gene21155	PPI
MS.gene64324	MS.gene060446	PPI
MS.gene64324	MS.gene07422	PPI
MS.gene62258	MS.gene64324	PPI
MS.gene64324	MS.gene023648	PPI
MS.gene64324	MS.gene062239	PPI
MS.gene64324	MS.gene013748	PPI
MS.gene64324	MS.gene64316	PPI
MS.gene64324	MS.gene98294	PPI
MS.gene64324	MS.gene22553	PPI
MS.gene64324	MS.gene061261	PPI
MS.gene64324	MS.gene03228	PPI
MS.gene64324	MS.gene46237	PPI
MS.gene64324	MS.gene64317	PPI
MS.gene64324	MS.gene46886	PPI
MS.gene64324	MS.gene040331	PPI
MS.gene58860	MS.gene64324	PPI
MS.gene64324	MS.gene015470	PPI
MS.gene64324	MS.gene55545	PPI
MS.gene64324	MS.gene63000	PPI
MS.gene97948	MS.gene64324	PPI
MS.gene64324	MS.gene29459	PPI
MS.gene64324	MS.gene71207	PPI
MS.gene64324	MS.gene67281	PPI
MS.gene64324	MS.gene92775	PPI
MS.gene04917	MS.gene64324	PPI
MS.gene64324	MS.gene74452	PPI
MS.gene64324	MS.gene09934	PPI
MS.gene64324	MS.gene27879	PPI
MS.gene64324	MS.gene53662	PPI
MS.gene64324	MS.gene63281	PPI
MS.gene64324	MS.gene68345	PPI
MS.gene64324	MS.gene003880	PPI
MS.gene64324	MS.gene008225	PPI
MS.gene64324	MS.gene000724	PPI
MS.gene64324	MS.gene97948	PPI
MS.gene64324	MS.gene21696	PPI
MS.gene64324	MS.gene64310	PPI
MS.gene64324	MS.gene29793	PPI
MS.gene64324	MS.gene022456	PPI
MS.gene64324	MS.gene05284	PPI
MS.gene64324	MS.gene001246	PPI
MS.gene64324	MS.gene53465	PPI
MS.gene64324	MS.gene074045	PPI
MS.gene64324	MS.gene000738	PPI
MS.gene64324	MS.gene85596	PPI
MS.gene64324	MS.gene96922	PPI
MS.gene64324	MS.gene04917	PPI
MS.gene64324	MS.gene056383	PPI
MS.gene64324	MS.gene64314	PPI
MS.gene64324	MS.gene025777	PPI
MS.gene64324	MS.gene94551	PPI
MS.gene64324	MS.gene022455	PPI
MS.gene64324	MS.gene038456	PPI
MS.gene64324	MS.gene93087	PPI
MS.gene003880	MS.gene64324	PPI
MS.gene64324	MS.gene046988	PPI
MS.gene54637	MS.gene64324	PPI
MS.gene64324	MS.gene074100	PPI
MS.gene64324	MS.gene057293	PPI
MS.gene64324	MS.gene53650	PPI
MS.gene64324	MS.gene04915	PPI
MS.gene46886	MS.gene64324	PPI
MS.gene64324	MS.gene54637	PPI
MS.gene64324	MS.gene58906	PPI
MS.gene64324	MS.gene79028	PPI
MS.gene64324	MS.gene004899	PPI
MS.gene64324	MS.gene64311	PPI
MS.gene64324	MS.gene49271	PPI
MS.gene64324	MS.gene053319	PPI
MS.gene64324	MS.gene026940	PPI
MS.gene64324	MS.gene65954	PPI
MS.gene64324	MS.gene98295	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64324.t1	MTR_1g046950	74.648	142	33	1	1	139	1	142	2.80e-70	207
MS.gene64324.t1	MTR_1g047100	74.286	140	34	1	1	138	1	140	6.79e-66	196
MS.gene64324.t1	MTR_5g079340	70.504	139	41	0	1	139	1	139	1.28e-65	196
MS.gene64324.t1	MTR_3g067610	64.964	137	45	1	3	139	4	137	1.36e-60	183
MS.gene64324.t1	MTR_5g008705	67.969	128	40	1	12	138	2	129	1.48e-52	162
MS.gene64324.t1	MTR_5g008695	68.696	115	33	1	28	139	1	115	6.27e-48	150
MS.gene64324.t1	MTR_8g107110	55.556	135	60	0	3	137	23	157	4.07e-45	145
MS.gene64324.t1	MTR_6g079570	51.095	137	66	1	3	138	25	161	2.13e-40	133
MS.gene64324.t1	MTR_1g032070	44.853	136	74	1	3	137	45	180	2.23e-35	121
MS.gene64324.t1	MTR_4g067270	46.043	139	70	3	3	137	4	141	1.06e-32	113
MS.gene64324.t1	MTR_8g105230	43.182	132	74	1	4	134	36	167	4.30e-32	112
MS.gene64324.t1	MTR_7g089760	45.985	137	71	2	4	137	5	141	1.43e-31	110
MS.gene64324.t1	MTR_7g075040	47.297	148	66	4	1	137	44	190	4.95e-31	110
MS.gene64324.t1	MTR_6g023460	41.985	131	76	0	7	137	11	141	2.35e-30	107
MS.gene64324.t1	MTR_8g036075	48.252	143	65	3	4	137	49	191	7.86e-30	107
MS.gene64324.t1	MTR_7g087610	38.519	135	82	1	4	137	12	146	1.74e-27	99.8
MS.gene64324.t1	MTR_7g087610	45.312	64	35	0	76	139	12	75	2.08e-12	60.8
MS.gene64324.t1	MTR_5g088320	39.259	135	81	1	4	137	12	146	1.99e-27	99.8
MS.gene64324.t1	MTR_5g088320	44.118	68	38	0	72	139	8	75	1.72e-12	61.2
MS.gene64324.t1	MTR_6g025320	39.259	135	81	1	4	137	12	146	2.08e-27	99.8
MS.gene64324.t1	MTR_6g025320	45.312	64	35	0	76	139	12	75	2.29e-12	60.8
MS.gene64324.t1	MTR_3g089090	38.931	131	80	0	7	137	8	138	4.84e-27	98.2
MS.gene64324.t1	MTR_3g089070	38.931	131	80	0	7	137	9	139	5.37e-27	98.2
MS.gene64324.t1	MTR_1g041285	40.000	135	80	1	4	137	12	146	5.41e-27	98.6
MS.gene64324.t1	MTR_1g076650	40.602	133	78	1	6	137	14	146	1.10e-26	97.8
MS.gene64324.t1	MTR_5g017510	37.500	136	84	1	3	137	47	182	1.47e-26	98.6
MS.gene64324.t1	MTR_5g017560	37.500	136	84	1	3	137	47	182	1.47e-26	98.6
MS.gene64324.t1	MTR_5g017550	37.037	135	85	0	3	137	47	181	1.06e-25	96.3
MS.gene64324.t1	MTR_4g103650	38.806	134	82	0	4	137	2	135	1.26e-25	94.7
MS.gene64324.t1	MTR_4g086260	38.686	137	83	1	3	139	56	191	2.69e-25	95.5
MS.gene64324.t1	MTR_4g103655	38.060	134	83	0	4	137	6	139	1.41e-24	92.0
MS.gene64324.t1	MTR_4g103630	38.060	134	83	0	4	137	6	139	2.18e-24	91.7
MS.gene64324.t1	MTR_7g090450	37.857	140	81	3	4	137	73	212	3.14e-23	90.5
MS.gene64324.t1	MTR_7g074240	42.177	147	70	5	5	137	25	170	8.08e-23	88.6
MS.gene64324.t1	MTR_3g083470	36.957	138	76	2	1	137	90	217	1.46e-22	89.4
MS.gene64324.t1	MTR_2g098890	40.714	140	78	2	3	137	63	202	1.56e-22	88.6
MS.gene64324.t1	MTR_4g112460	33.571	140	90	1	3	139	10	149	3.24e-22	86.7
MS.gene64324.t1	MTR_8g066630	32.609	138	89	1	4	137	17	154	1.07e-21	85.5
MS.gene64324.t1	MTR_0168s0120	38.182	110	67	1	29	137	1	110	6.81e-21	82.0
MS.gene64324.t1	MTR_6g025320	38.182	110	67	1	29	137	1	110	6.81e-21	82.0
MS.gene64324.t1	MTR_4g115170	38.312	154	76	3	3	137	63	216	2.46e-20	83.2
MS.gene64324.t1	MTR_1g071150	37.209	129	81	0	9	137	17	145	5.49e-20	80.5
MS.gene64324.t1	MTR_8g069915	29.496	139	95	1	4	139	12	150	1.80e-19	79.7
MS.gene64324.t1	MTR_5g092810	37.500	136	78	2	5	137	398	529	9.21e-19	81.6
MS.gene64324.t1	MTR_8g078270	33.333	135	89	1	5	138	54	188	9.63e-19	78.2
MS.gene64324.t1	MTR_4g132070	35.294	136	81	2	5	137	397	528	3.91e-18	79.7
MS.gene64324.t1	MTR_5g009830	34.783	138	83	2	5	139	372	505	3.93e-18	79.7
MS.gene64324.t1	MTR_6g007613	34.058	138	88	2	4	139	29	165	4.69e-18	76.3
MS.gene64324.t1	MTR_6g007613	37.500	80	47	1	59	138	15	91	5.50e-12	60.1
MS.gene64324.t1	MTR_1g026190	34.058	138	84	2	5	139	388	521	5.57e-18	79.3
MS.gene64324.t1	MTR_8g099095	34.058	138	84	2	5	139	372	505	6.51e-18	79.3
MS.gene64324.t1	MTR_7g115040	35.385	130	83	1	9	137	17	146	8.30e-18	75.1
MS.gene64324.t1	MTR_7g074020	33.088	136	88	2	4	137	30	164	1.98e-17	74.3
MS.gene64324.t1	MTR_7g074020	40.299	67	40	0	72	138	26	92	7.49e-12	59.7
MS.gene64324.t1	MTR_3g051770	33.793	145	87	2	2	137	383	527	2.96e-17	77.4
MS.gene64324.t1	MTR_5g027000	33.071	127	84	1	9	134	27	153	2.59e-16	71.2
MS.gene64324.t1	MTR_1g030440	33.562	146	80	2	3	137	21	160	9.25e-16	70.1
MS.gene64324.t1	MTR_5g089320	30.370	135	93	1	5	139	374	507	6.79e-15	70.5
MS.gene64324.t1	MTR_5g011850	31.469	143	91	4	2	139	7	147	1.21e-14	66.6
MS.gene64324.t1	MTR_4g107490	33.582	134	87	2	5	137	379	511	1.75e-14	69.3
MS.gene64324.t1	MTR_5g079470	51.471	68	32	1	7	74	13	79	3.34e-14	63.9
MS.gene64324.t1	MTR_3g055570	30.370	135	80	1	4	138	52	172	5.81e-14	65.5
MS.gene64324.t1	MTR_3g088655	32.468	154	77	5	4	137	8	154	1.00e-13	64.7
MS.gene64324.t1	MTR_7g074020	30.894	123	82	2	4	124	30	151	1.02e-13	64.7
MS.gene64324.t1	MTR_7g074020	40.299	67	40	0	72	138	26	92	7.62e-12	59.7
MS.gene64324.t1	MTR_0028s0170	29.710	138	90	2	5	139	384	517	1.07e-13	67.0
MS.gene64324.t1	MTR_6g007613	31.707	123	81	2	4	124	29	150	1.23e-13	64.3
MS.gene64324.t1	MTR_6g007613	37.500	80	47	1	59	138	15	91	4.45e-12	60.1
MS.gene64324.t1	MTR_4g132040	31.579	133	90	1	5	137	401	532	2.12e-13	66.2
MS.gene64324.t1	MTR_7g068710	31.852	135	92	0	3	137	360	494	4.44e-13	65.5
MS.gene64324.t1	MTR_1g046960	62.264	53	18	1	28	78	1	53	5.67e-13	61.2
MS.gene64324.t1	MTR_7g091890	34.109	129	85	0	9	137	368	496	4.18e-12	62.4
MS.gene64324.t1	MTR_1g054865	29.851	134	90	2	5	137	467	597	4.19e-12	62.4
MS.gene64324.t1	MTR_1g096490	30.657	137	89	2	5	139	336	468	9.48e-12	61.6
MS.gene64324.t1	MTR_8g096050	27.941	136	93	1	2	137	53	183	4.72e-11	59.7
MS.gene64324.t1	MTR_4g066660	31.387	137	89	2	3	137	376	509	6.58e-11	58.9
MS.gene64324.t1	MTR_3g098460	30.534	131	86	1	4	134	33	158	6.82e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64324.t1	AT1G18210	50.370	135	64	2	3	137	22	153	2.97e-40	132
MS.gene64324.t1	AT1G18210	50.370	135	64	2	3	137	22	153	2.97e-40	132
MS.gene64324.t1	AT1G73630	48.905	137	67	2	2	138	18	151	7.65e-39	129
MS.gene64324.t1	AT5G37770	46.809	141	70	1	3	138	16	156	8.13e-39	129
MS.gene64324.t1	AT5G37770	46.809	141	70	1	3	138	16	156	8.13e-39	129
MS.gene64324.t1	AT1G66400	45.652	138	72	1	3	137	14	151	1.42e-38	128
MS.gene64324.t1	AT5G17470	44.526	137	73	1	5	138	3	139	1.81e-34	117
MS.gene64324.t1	AT1G24620	44.118	136	75	1	4	138	37	172	4.16e-33	115
MS.gene64324.t1	AT2G15680	43.382	136	76	1	3	138	49	183	1.02e-32	114
MS.gene64324.t1	AT3G07490	47.445	137	69	2	4	137	5	141	3.93e-32	111
MS.gene64324.t1	AT2G36180	46.269	134	68	1	8	137	4	137	8.02e-32	110
MS.gene64324.t1	AT1G05990	44.928	138	72	2	4	137	5	142	3.78e-30	106
MS.gene64324.t1	AT4G03290	40.845	142	78	2	4	139	5	146	2.12e-28	102
MS.gene64324.t1	AT5G37780	40.000	135	80	1	4	137	12	146	3.96e-28	101
MS.gene64324.t1	AT5G37780	42.647	68	39	0	72	139	8	75	6.20e-12	59.7
MS.gene64324.t1	AT1G66410	40.000	135	80	1	4	137	12	146	3.96e-28	101
MS.gene64324.t1	AT1G66410	42.647	68	39	0	72	139	8	75	6.20e-12	59.7
MS.gene64324.t1	AT3G59440	42.754	138	73	3	4	137	51	186	6.65e-28	102
MS.gene64324.t1	AT5G21274	40.000	135	80	1	4	137	12	146	8.65e-28	100
MS.gene64324.t1	AT5G21274	45.312	64	35	0	76	139	12	75	2.45e-12	60.8
MS.gene64324.t1	AT3G43810	39.259	135	81	1	4	137	12	146	2.17e-27	99.8
MS.gene64324.t1	AT3G43810	45.312	64	35	0	76	139	12	75	2.40e-12	60.8
MS.gene64324.t1	AT3G56800	39.259	135	81	1	4	137	12	146	2.37e-27	99.4
MS.gene64324.t1	AT3G56800	45.312	64	35	0	76	139	12	75	2.37e-12	60.8
MS.gene64324.t1	AT2G27030	39.259	135	81	1	4	137	12	146	2.37e-27	99.4
MS.gene64324.t1	AT2G27030	45.312	64	35	0	76	139	12	75	2.37e-12	60.8
MS.gene64324.t1	AT2G41110	39.259	135	81	1	4	137	12	146	2.37e-27	99.4
MS.gene64324.t1	AT2G41110	45.312	64	35	0	76	139	12	75	2.37e-12	60.8
MS.gene64324.t1	AT2G43290	40.141	142	77	2	4	137	65	206	4.01e-27	100
MS.gene64324.t1	AT2G27030	39.259	135	81	1	4	137	12	146	5.21e-27	99.4
MS.gene64324.t1	AT2G27030	45.312	64	35	0	76	139	12	75	6.20e-12	60.5
MS.gene64324.t1	AT3G43810	39.259	135	81	1	4	137	12	146	1.36e-26	99.4
MS.gene64324.t1	AT3G43810	45.312	64	35	0	76	139	12	75	1.01e-11	60.5
MS.gene64324.t1	AT3G10190	41.176	136	78	2	4	137	70	205	1.57e-26	99.0
MS.gene64324.t1	AT4G12860	42.336	137	76	2	4	137	5	141	1.62e-26	97.4
MS.gene64324.t1	AT4G37010	39.130	138	81	2	4	139	27	163	8.95e-26	95.9
MS.gene64324.t1	AT4G37010	39.130	138	81	2	4	139	31	167	1.10e-25	95.9
MS.gene64324.t1	AT3G22930	39.259	135	81	1	4	137	35	169	2.12e-25	95.1
MS.gene64324.t1	AT3G03400	37.209	129	78	1	5	130	6	134	2.54e-25	94.0
MS.gene64324.t1	AT2G41110	36.054	147	81	2	4	137	12	158	4.13e-24	91.7
MS.gene64324.t1	AT5G37780	36.000	150	80	2	4	137	12	161	6.36e-24	91.3
MS.gene64324.t1	AT4G14640	37.778	135	83	1	4	137	13	147	1.24e-23	90.1
MS.gene64324.t1	AT3G50360	36.957	138	84	2	4	139	27	163	1.34e-23	90.5
MS.gene64324.t1	AT3G50360	40.845	71	42	0	68	138	19	89	1.18e-12	62.0
MS.gene64324.t1	AT5G37780	38.843	121	73	1	18	137	13	133	7.71e-23	87.4
MS.gene64324.t1	AT1G66410	38.843	121	73	1	18	137	36	156	1.25e-22	87.8
MS.gene64324.t1	AT3G50770	35.664	143	84	3	3	138	63	204	1.52e-22	88.6
MS.gene64324.t1	AT5G37780	38.843	121	73	1	18	137	52	172	1.90e-22	87.8
MS.gene64324.t1	AT1G32250	34.028	144	84	2	3	137	15	156	2.17e-22	87.4
MS.gene64324.t1	AT1G76650	35.821	134	86	0	4	137	43	176	5.47e-22	86.7
MS.gene64324.t1	AT1G76650	35.821	134	86	0	4	137	43	176	5.47e-22	86.7
MS.gene64324.t1	AT1G66410	39.091	110	66	1	29	137	1	110	1.15e-21	84.0
MS.gene64324.t1	AT1G66410	39.091	110	66	1	29	137	1	110	1.15e-21	84.0
MS.gene64324.t1	AT1G18530	32.414	145	92	2	1	139	1	145	4.70e-21	83.6
MS.gene64324.t1	AT3G43810	38.182	110	67	1	29	137	1	110	7.13e-21	82.0
MS.gene64324.t1	AT3G43810	38.182	110	67	1	29	137	1	110	7.13e-21	82.0
MS.gene64324.t1	AT2G41410	35.971	139	85	3	4	138	70	208	2.73e-20	83.2
MS.gene64324.t1	AT2G41100	33.333	156	81	2	4	139	12	164	1.20e-19	83.2
MS.gene64324.t1	AT2G41100	32.903	155	82	2	4	138	101	253	7.83e-19	80.9
MS.gene64324.t1	AT2G41100	33.333	156	81	2	4	139	12	164	1.20e-19	83.2
MS.gene64324.t1	AT2G41100	32.903	155	82	2	4	138	101	253	7.83e-19	80.9
MS.gene64324.t1	AT4G20780	34.416	154	80	3	5	137	30	183	1.24e-19	80.9
MS.gene64324.t1	AT2G41090	34.815	135	84	2	4	137	12	143	2.72e-19	79.7
MS.gene64324.t1	AT4G14640	36.364	121	76	1	18	137	9	129	2.98e-19	78.2
MS.gene64324.t1	AT5G42380	34.307	137	88	1	3	137	48	184	3.45e-19	79.3
MS.gene64324.t1	AT2G41100	32.903	155	82	2	4	138	66	218	4.20e-19	81.3
MS.gene64324.t1	AT2G41100	29.545	132	70	2	28	139	1	129	9.16e-12	61.2
MS.gene64324.t1	AT2G41100	32.903	155	82	2	4	138	66	218	4.20e-19	81.3
MS.gene64324.t1	AT2G41100	29.545	132	70	2	28	139	1	129	9.16e-12	61.2
MS.gene64324.t1	AT2G41100	32.903	155	82	2	4	138	66	218	4.20e-19	81.3
MS.gene64324.t1	AT2G41100	29.545	132	70	2	28	139	1	129	9.16e-12	61.2
MS.gene64324.t1	AT1G76650	35.821	134	76	2	4	137	43	166	6.44e-19	78.2
MS.gene64324.t1	AT4G04700	35.338	133	85	1	5	137	337	468	1.04e-18	81.3
MS.gene64324.t1	AT3G25600	30.935	139	93	1	4	139	12	150	1.37e-18	77.4
MS.gene64324.t1	AT1G61950	35.294	136	81	2	5	137	405	536	2.53e-18	80.5
MS.gene64324.t1	AT4G04695	34.559	136	82	2	5	137	337	468	5.09e-18	79.3
MS.gene64324.t1	AT3G20410	36.765	136	79	2	5	137	397	528	5.32e-18	79.3
MS.gene64324.t1	AT3G20410	36.765	136	79	2	5	137	397	528	5.32e-18	79.3
MS.gene64324.t1	AT4G23650	33.333	138	85	2	5	139	384	517	1.74e-17	78.2
MS.gene64324.t1	AT3G03410	32.331	133	82	2	5	137	3	127	2.15e-17	73.6
MS.gene64324.t1	AT4G04740	35.294	136	81	2	5	137	374	505	2.19e-17	77.8
MS.gene64324.t1	AT4G04740	35.294	136	81	2	5	137	374	505	2.19e-17	77.8
MS.gene64324.t1	AT4G04740	35.294	136	81	2	5	137	374	505	2.19e-17	77.8
MS.gene64324.t1	AT4G04740	35.294	136	81	2	5	137	374	505	2.19e-17	77.8
MS.gene64324.t1	AT4G04740	35.294	136	81	2	5	137	374	505	2.19e-17	77.8
MS.gene64324.t1	AT4G04740	35.294	136	81	2	5	137	374	505	2.19e-17	77.8
MS.gene64324.t1	AT1G50700	35.294	136	81	2	5	137	379	510	2.30e-17	77.8
MS.gene64324.t1	AT4G21940	35.294	136	81	2	5	137	407	538	3.68e-17	77.0
MS.gene64324.t1	AT3G01830	35.000	140	82	4	3	137	10	145	3.72e-17	73.2
MS.gene64324.t1	AT4G21940	35.294	136	81	2	5	137	407	538	3.80e-17	77.0
MS.gene64324.t1	AT1G74740	39.706	136	76	3	5	137	365	497	5.77e-17	76.6
MS.gene64324.t1	AT4G04720	34.559	136	82	2	5	137	385	516	6.52e-17	76.3
MS.gene64324.t1	AT4G04720	34.559	136	82	2	5	137	385	516	6.52e-17	76.3
MS.gene64324.t1	AT3G51920	31.429	140	92	2	2	137	7	146	8.99e-17	72.4
MS.gene64324.t1	AT1G76640	35.075	134	82	2	8	137	26	158	9.84e-17	72.4
MS.gene64324.t1	AT1G21550	36.667	150	74	4	3	137	9	152	1.61e-16	71.6
MS.gene64324.t1	AT1G76040	34.783	138	83	2	5	139	179	312	1.80e-16	74.3
MS.gene64324.t1	AT1G76040	34.783	138	83	2	5	139	179	312	1.80e-16	74.3
MS.gene64324.t1	AT5G44460	34.459	148	80	3	5	137	29	174	1.87e-16	72.4
MS.gene64324.t1	AT3G10660	29.630	135	93	1	5	139	492	624	3.08e-16	74.3
MS.gene64324.t1	AT3G10660	29.630	135	93	1	5	139	492	624	3.08e-16	74.3
MS.gene64324.t1	AT1G76040	34.783	138	83	2	5	139	417	550	3.23e-16	74.3
MS.gene64324.t1	AT3G51850	32.593	135	91	0	3	137	358	492	4.70e-15	70.9
MS.gene64324.t1	AT1G18890	36.029	136	81	3	5	137	369	501	6.63e-15	70.5
MS.gene64324.t1	AT5G19360	31.852	135	91	1	5	139	374	507	6.98e-15	70.5
MS.gene64324.t1	AT5G17480	53.731	67	30	1	8	74	13	78	7.72e-15	65.5
MS.gene64324.t1	AT4G04710	33.824	136	83	2	5	137	338	469	8.90e-15	70.1
MS.gene64324.t1	AT4G04710	33.824	136	83	2	5	137	324	455	8.91e-15	70.1
MS.gene64324.t1	AT1G12310	32.168	143	90	4	2	139	8	148	9.73e-15	67.0
MS.gene64324.t1	AT3G03000	33.088	136	89	1	4	137	20	155	1.11e-14	67.4
MS.gene64324.t1	AT4G04710	33.824	136	83	2	5	137	351	482	1.16e-14	70.1
MS.gene64324.t1	AT1G62820	31.387	137	90	3	5	139	14	148	1.54e-14	66.6
MS.gene64324.t1	AT5G12180	31.111	135	92	1	5	139	379	512	4.22e-14	68.2
MS.gene64324.t1	AT5G04870	29.927	137	90	2	5	139	456	588	9.56e-14	67.4
MS.gene64324.t1	AT4G04710	33.333	138	83	3	5	137	351	484	1.62e-13	66.6
MS.gene64324.t1	AT3G03430	47.761	67	34	1	8	74	13	78	4.99e-13	60.8
MS.gene64324.t1	AT3G22930	35.417	96	61	1	4	98	35	130	2.12e-12	60.5
MS.gene64324.t1	AT2G41860	32.331	133	90	0	5	137	360	492	9.57e-12	61.6
MS.gene64324.t1	AT2G41860	32.331	133	90	0	5	137	360	492	1.00e-11	61.6
MS.gene64324.t1	AT5G66210	31.915	141	83	5	4	137	369	503	1.53e-11	60.8
MS.gene64324.t1	AT5G66210	31.915	141	83	5	4	137	369	503	1.53e-11	60.8
MS.gene64324.t1	AT1G35670	30.657	137	89	2	5	139	332	464	1.88e-11	60.8
MS.gene64324.t1	AT4G09570	30.657	137	89	2	5	139	331	463	2.28e-11	60.5
MS.gene64324.t1	AT5G23580	28.148	135	95	1	5	139	328	460	3.44e-11	60.1
MS.gene64324.t1	AT3G57530	33.582	134	86	2	5	137	369	500	3.89e-11	59.7
MS.gene64324.t1	AT5G07320	28.571	133	90	1	5	137	38	165	4.32e-11	59.7
MS.gene64324.t1	AT2G17890	30.496	141	85	5	4	137	415	549	5.46e-11	59.3
MS.gene64324.t1	AT2G41100	29.688	128	68	2	31	138	3	128	6.50e-11	57.8
MS.gene64324.t1	AT5G12480	31.343	134	91	1	5	137	365	498	8.92e-11	58.9
MS.gene64324.t1	AT4G36070	29.577	142	87	5	3	137	377	512	9.45e-11	58.5
MS.gene64324.t1	AT5G12480	31.343	134	91	1	5	137	271	404	9.45e-11	58.5

Find 28 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACATAGTTCTTAATAAACT+TGG	0.359582	1.3:-28730561	MS.gene64324:CDS
AGAACTATGTGCAATTCTTT+CGG	0.363167	1.3:+28730573	None:intergenic
AAAGATATCGTACTCGGAAC+TGG	0.436060	1.3:-28730799	MS.gene64324:CDS
AGCACCGGTGCAGTAGAAAT+CGG	0.483491	1.3:+28730668	None:intergenic
GAGGAATTGCTTTCAACACT+TGG	0.485807	1.3:-28730777	MS.gene64324:CDS
TGATGAAGTAATAAAGATGA+TGG	0.523203	1.3:-28730739	MS.gene64324:CDS
CGTCCTTGTCGACGTCGTAA+AGG	0.532322	1.3:+28730607	None:intergenic
AAGTTCGACAAGAACGGTGA+TGG	0.542287	1.3:-28730822	MS.gene64324:CDS
AGAAGATGATGGCTCGCTGA+TGG	0.550707	1.3:-28730452	None:intergenic
CGAGGAGTTCAAGAAGATGA+TGG	0.561757	1.3:-28730463	MS.gene64324:CDS
CGACCTTTACGACGTCGACA+AGG	0.572934	1.3:-28730610	MS.gene64324:CDS
GTTGCGCACCTCGTCATCCA+TGG	0.582107	1.3:+28730854	None:intergenic
ACGTTGATGCTGATGGTGAC+GGG	0.590220	1.3:-28730497	MS.gene64324:CDS
TTTGCCGATTTCTACTGCAC+CGG	0.595871	1.3:-28730672	MS.gene64324:CDS
GACGTTGATGCTGATGGTGA+CGG	0.609410	1.3:-28730498	MS.gene64324:CDS
TGACGGGAAAGTCAACTTCG+AGG	0.611813	1.3:-28730481	MS.gene64324:CDS
TTCAACAAGTTCGACAAGAA+CGG	0.613960	1.3:-28730828	MS.gene64324:CDS
CTTGGTGAGAAGTACTCCCT+CGG	0.619034	1.3:-28730543	MS.gene64324:CDS
CATTCTCTCACAGTCACCGA+GGG	0.627783	1.3:+28730527	None:intergenic
ATGGAAGATATTGATCAGAA+CGG	0.635154	1.3:-28730720	MS.gene64324:CDS
CTTTACGACGTCGACAAGGA+CGG	0.635439	1.3:-28730606	MS.gene64324:CDS
GATATCGTACTCGGAACTGG+AGG	0.635943	1.3:-28730796	MS.gene64324:CDS
GATATTGATCAGAACGGCCA+CGG	0.641453	1.3:-28730714	MS.gene64324:CDS
AAACTCTGCCATGGATGACG+AGG	0.643225	1.3:-28730862	None:intergenic
TGATGGAAAGATATCGTACT+CGG	0.658411	1.3:-28730805	MS.gene64324:CDS
ATATGCGACGTTGATGCTGA+TGG	0.659619	1.3:-28730504	MS.gene64324:CDS
CTTTAAGATCAACGTAACCG+TGG	0.696038	1.3:+28730697	None:intergenic
TCATTCTCTCACAGTCACCG+AGG	0.702111	1.3:+28730526	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CACATAGTTCTTAATAAACT+TGG	-	chr1.3:28730746-28730765	MS.gene64324:CDS	25.0%
!	TGATGAAGTAATAAAGATGA+TGG	-	chr1.3:28730568-28730587	MS.gene64324:CDS	25.0%
	AGAACTATGTGCAATTCTTT+CGG	+	chr1.3:28730737-28730756	None:intergenic	30.0%
	ATGGAAGATATTGATCAGAA+CGG	-	chr1.3:28730587-28730606	MS.gene64324:CDS	30.0%
	TGATGGAAAGATATCGTACT+CGG	-	chr1.3:28730502-28730521	MS.gene64324:CDS	35.0%
	TTCAACAAGTTCGACAAGAA+CGG	-	chr1.3:28730479-28730498	MS.gene64324:CDS	35.0%
	AAAGATATCGTACTCGGAAC+TGG	-	chr1.3:28730508-28730527	MS.gene64324:CDS	40.0%
	GAGGAATTGCTTTCAACACT+TGG	-	chr1.3:28730530-28730549	MS.gene64324:CDS	40.0%
!!	CTTTAAGATCAACGTAACCG+TGG	+	chr1.3:28730613-28730632	None:intergenic	40.0%
	AAGTTCGACAAGAACGGTGA+TGG	-	chr1.3:28730485-28730504	MS.gene64324:CDS	45.0%
	ATATGCGACGTTGATGCTGA+TGG	-	chr1.3:28730803-28730822	MS.gene64324:CDS	45.0%
	CGAGGAGTTCAAGAAGATGA+TGG	-	chr1.3:28730844-28730863	MS.gene64324:CDS	45.0%
	GATATTGATCAGAACGGCCA+CGG	-	chr1.3:28730593-28730612	MS.gene64324:CDS	45.0%
!!	TTTGCCGATTTCTACTGCAC+CGG	-	chr1.3:28730635-28730654	MS.gene64324:CDS	45.0%
	CATTCTCTCACAGTCACCGA+GGG	+	chr1.3:28730783-28730802	None:intergenic	50.0%
	CTTTACGACGTCGACAAGGA+CGG	-	chr1.3:28730701-28730720	MS.gene64324:CDS	50.0%
	GATATCGTACTCGGAACTGG+AGG	-	chr1.3:28730511-28730530	MS.gene64324:CDS	50.0%
	TCATTCTCTCACAGTCACCG+AGG	+	chr1.3:28730784-28730803	None:intergenic	50.0%
	TGACGGGAAAGTCAACTTCG+AGG	-	chr1.3:28730826-28730845	MS.gene64324:CDS	50.0%
	TGCTGCAACCGAAAAAGACG+AGG	-	chr1.3:28730658-28730677	MS.gene64324:CDS	50.0%
!	ACGTTGATGCTGATGGTGAC+GGG	-	chr1.3:28730810-28730829	MS.gene64324:CDS	50.0%
!	GACGTTGATGCTGATGGTGA+CGG	-	chr1.3:28730809-28730828	MS.gene64324:CDS	50.0%
!	GCGAAGCTCCTCGTCTTTTT+CGG	+	chr1.3:28730669-28730688	None:intergenic	50.0%
!!	AGCACCGGTGCAGTAGAAAT+CGG	+	chr1.3:28730642-28730661	None:intergenic	50.0%
!!	CTTGGTGAGAAGTACTCCCT+CGG	-	chr1.3:28730764-28730783	MS.gene64324:CDS	50.0%
!!!	GTCTTTTTCGGTTGCAGCAC+CGG	+	chr1.3:28730657-28730676	None:intergenic	50.0%
	CGACCTTTACGACGTCGACA+AGG	-	chr1.3:28730697-28730716	MS.gene64324:CDS	55.0%
	CGTCCTTGTCGACGTCGTAA+AGG	+	chr1.3:28730703-28730722	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	28730455	28730874	28730455	ID=MS.gene64324
chr1.3	mRNA	28730455	28730874	28730455	ID=MS.gene64324.t1;Parent=MS.gene64324
chr1.3	exon	28730455	28730874	28730455	ID=MS.gene64324.t1.exon1;Parent=MS.gene64324.t1
chr1.3	CDS	28730455	28730874	28730455	ID=cds.MS.gene64324.t1;Parent=MS.gene64324.t1

Gene Sequence

>MS.gene64324

ATGGATGACGAGGTGCGCAACATCTTCAACAAGTTCGACAAGAACGGTGATGGAAAGATATCGTACTCGGAACTGGAGGAATTGCTTTCAACACTTGGAAGCAAAACAACATTTGATGAAGTAATAAAGATGATGGAAGATATTGATCAGAACGGCCACGGTTACGTTGATCTTAAAGAATTTGCCGATTTCTACTGCACCGGTGCTGCAACCGAAAAAGACGAGGAGCTTCGCTATGCTTTCGACCTTTACGACGTCGACAAGGACGGTCTCATCTCTCCGAAAGAATTGCACATAGTTCTTAATAAACTTGGTGAGAAGTACTCCCTCGGTGACTGTGAGAGAATGATATGCGACGTTGATGCTGATGGTGACGGGAAAGTCAACTTCGAGGAGTTCAAGAAGATGATGGCTCGCTGA

Protein sequence

>MS.gene64324.t1

MDDEVRNIFNKFDKNGDGKISYSELEELLSTLGSKTTFDEVIKMMEDIDQNGHGYVDLKEFADFYCTGAATEKDEELRYAFDLYDVDKDGLISPKELHIVLNKLGEKYSLGDCERMICDVDADGDGKVNFEEFKKMMAR