AlfalfaGEDB | MS.gene033361

M. sativa cultivar XinJiangDaYe / MS.gene033361

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene033361.t1	XP_003608847.1	95.1	41	2	0	1	41	1	41	1.80E-12	82.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene033361.t1	G7JLF5	95.1	41	2	0	1	41	1	41	1.3e-12	82.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene033361	MS.gene79028	PPI
MS.gene066862	MS.gene033361	PPI
MS.gene015658	MS.gene033361	PPI
MS.gene033361	MS.gene21696	PPI
MS.gene024717	MS.gene033361	PPI
MS.gene64308	MS.gene033361	PPI
MS.gene64313	MS.gene033361	PPI
MS.gene83068	MS.gene033361	PPI
MS.gene93087	MS.gene033361	PPI
MS.gene62689	MS.gene033361	PPI
MS.gene97948	MS.gene033361	PPI
MS.gene003880	MS.gene033361	PPI
MS.gene54637	MS.gene033361	PPI
MS.gene000738	MS.gene033361	PPI
MS.gene033361	MS.gene025797	PPI
MS.gene67281	MS.gene033361	PPI
MS.gene061261	MS.gene033361	PPI
MS.gene033361	MS.gene64314	PPI
MS.gene025777	MS.gene033361	PPI
MS.gene062239	MS.gene033361	PPI
MS.gene46886	MS.gene033361	PPI
MS.gene026530	MS.gene033361	PPI
MS.gene21155	MS.gene033361	PPI
MS.gene71207	MS.gene033361	PPI
MS.gene033361	MS.gene53650	PPI
MS.gene07422	MS.gene033361	PPI
MS.gene03228	MS.gene033361	PPI
MS.gene21932	MS.gene033361	PPI
MS.gene28338	MS.gene033361	PPI
MS.gene85596	MS.gene033361	PPI
MS.gene94551	MS.gene033361	PPI
MS.gene65954	MS.gene033361	PPI
MS.gene008225	MS.gene033361	PPI
MS.gene056383	MS.gene033361	PPI
MS.gene64324	MS.gene033361	PPI
MS.gene015470	MS.gene033361	PPI
MS.gene63000	MS.gene033361	PPI
MS.gene22553	MS.gene033361	PPI
MS.gene53662	MS.gene033361	PPI
MS.gene80011	MS.gene033361	PPI
MS.gene96914	MS.gene033361	PPI
MS.gene070168	MS.gene033361	PPI
MS.gene033361	MS.gene21931	PPI
MS.gene033361	MS.gene53659	PPI
MS.gene033361	MS.gene09960	PPI
MS.gene033361	MS.gene061768	PPI
MS.gene033361	MS.gene89216	PPI
MS.gene026940	MS.gene033361	PPI
MS.gene92775	MS.gene033361	PPI
MS.gene004899	MS.gene033361	PPI
MS.gene80013	MS.gene033361	PPI
MS.gene09934	MS.gene033361	PPI
MS.gene96922	MS.gene033361	PPI
MS.gene009021	MS.gene033361	PPI
MS.gene46237	MS.gene033361	PPI
MS.gene070167	MS.gene033361	PPI
MS.gene033361	MS.gene93763	PPI
MS.gene033361	MS.gene80012	PPI
MS.gene87673	MS.gene033361	PPI
MS.gene05284	MS.gene033361	PPI
MS.gene21931	MS.gene033361	PPI
MS.gene041795	MS.gene033361	PPI
MS.gene046988	MS.gene033361	PPI
MS.gene057293	MS.gene033361	PPI
MS.gene63281	MS.gene033361	PPI
MS.gene53648	MS.gene033361	PPI
MS.gene68345	MS.gene033361	PPI
MS.gene017446	MS.gene033361	PPI
MS.gene066863	MS.gene033361	PPI
MS.gene033361	MS.gene056461	PPI
MS.gene033361	MS.gene98972	PPI
MS.gene89216	MS.gene033361	PPI
MS.gene001246	MS.gene033361	PPI
MS.gene040127	MS.gene033361	PPI
MS.gene62258	MS.gene033361	PPI
MS.gene033361	MS.gene29459	PPI
MS.gene64310	MS.gene033361	PPI
MS.gene53659	MS.gene033361	PPI
MS.gene038456	MS.gene033361	PPI
MS.gene000724	MS.gene033361	PPI
MS.gene052672	MS.gene033361	PPI
MS.gene64311	MS.gene033361	PPI
MS.gene25186	MS.gene033361	PPI
MS.gene008831	MS.gene033361	PPI
MS.gene96913	MS.gene033361	PPI
MS.gene58906	MS.gene033361	PPI
MS.gene020515	MS.gene033361	PPI
MS.gene033361	MS.gene64316	PPI
MS.gene58860	MS.gene033361	PPI
MS.gene27516	MS.gene033361	PPI
MS.gene02545	MS.gene033361	PPI
MS.gene053319	MS.gene033361	PPI
MS.gene04915	MS.gene033361	PPI
MS.gene61009	MS.gene033361	PPI
MS.gene64327	MS.gene033361	PPI
MS.gene64307	MS.gene033361	PPI
MS.gene033361	MS.gene64317	PPI
MS.gene026618	MS.gene033361	PPI
MS.gene04917	MS.gene033361	PPI
MS.gene64316	MS.gene033361	PPI
MS.gene074045	MS.gene033361	PPI
MS.gene47195	MS.gene033361	PPI
MS.gene036930	MS.gene033361	PPI
MS.gene033361	MS.gene64310	PPI
MS.gene013748	MS.gene033361	PPI
MS.gene55545	MS.gene033361	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene033361.t1	MTR_4g103630	95.714	140	6	0	1	140	1	140	1.89e-91	261
MS.gene033361.t1	MTR_4g103655	95.000	140	7	0	1	140	1	140	1.08e-90	259
MS.gene033361.t1	MTR_4g103650	96.324	136	5	0	5	140	1	136	1.96e-88	253
MS.gene033361.t1	MTR_3g089070	73.571	140	37	0	1	140	1	140	9.95e-69	204
MS.gene033361.t1	MTR_3g089090	74.453	137	35	0	4	140	3	139	3.18e-68	202
MS.gene033361.t1	MTR_6g023460	59.701	134	54	0	6	139	8	141	1.10e-54	168
MS.gene033361.t1	MTR_8g078270	54.135	133	56	2	10	139	57	187	7.90e-38	127
MS.gene033361.t1	MTR_3g067610	42.537	134	74	1	6	139	5	135	1.73e-30	107
MS.gene033361.t1	MTR_8g107110	39.259	135	82	0	6	140	24	158	1.35e-27	100
MS.gene033361.t1	MTR_8g105230	43.182	132	74	1	6	136	36	167	1.92e-27	100
MS.gene033361.t1	MTR_5g079340	38.806	134	82	0	6	139	4	137	6.79e-27	97.8
MS.gene033361.t1	MTR_4g067270	39.130	138	81	2	6	140	5	142	1.96e-25	94.7
MS.gene033361.t1	MTR_1g032070	40.741	135	79	1	6	139	46	180	1.68e-24	93.6
MS.gene033361.t1	MTR_7g075040	38.000	150	83	3	1	140	42	191	1.91e-23	90.9
MS.gene033361.t1	MTR_1g046950	34.752	141	81	2	6	139	4	140	2.28e-23	89.0
MS.gene033361.t1	MTR_1g047100	34.559	136	87	1	6	139	4	139	6.68e-23	87.8
MS.gene033361.t1	MTR_6g079570	37.037	135	84	1	6	139	26	160	3.61e-22	87.0
MS.gene033361.t1	MTR_3g083470	38.519	135	72	3	6	139	93	217	4.34e-21	85.5
MS.gene033361.t1	MTR_7g087610	35.000	140	81	3	6	139	12	147	7.52e-21	82.8
MS.gene033361.t1	MTR_6g025320	35.714	140	80	3	6	139	12	147	8.55e-21	82.8
MS.gene033361.t1	MTR_5g088320	35.714	140	80	3	6	139	12	147	9.32e-21	82.4
MS.gene033361.t1	MTR_4g086260	35.821	134	85	1	6	139	57	189	1.09e-20	83.6
MS.gene033361.t1	MTR_1g076650	34.286	140	83	2	5	139	11	146	7.79e-20	80.1
MS.gene033361.t1	MTR_8g036075	35.417	144	84	3	6	140	49	192	3.62e-19	79.7
MS.gene033361.t1	MTR_7g089760	34.783	138	87	2	6	140	5	142	4.91e-19	78.2
MS.gene033361.t1	MTR_7g090450	33.793	145	82	4	6	140	73	213	6.10e-19	79.3
MS.gene033361.t1	MTR_1g041285	33.824	136	89	1	5	139	11	146	1.11e-18	77.4
MS.gene033361.t1	MTR_8g066630	32.847	137	82	2	6	135	17	150	3.99e-18	76.3
MS.gene033361.t1	MTR_2g098890	37.143	140	83	2	6	140	64	203	7.10e-18	76.6
MS.gene033361.t1	MTR_1g071150	32.593	135	90	1	6	139	12	146	7.36e-18	75.1
MS.gene033361.t1	MTR_5g017510	33.333	135	89	1	6	139	48	182	7.72e-18	75.9
MS.gene033361.t1	MTR_5g017560	33.333	135	89	1	6	139	48	182	7.72e-18	75.9
MS.gene033361.t1	MTR_7g074240	32.667	150	88	2	3	139	21	170	4.91e-17	73.6
MS.gene033361.t1	MTR_4g115170	33.766	154	83	3	6	140	64	217	3.33e-16	72.4
MS.gene033361.t1	MTR_5g017550	32.353	136	88	2	6	139	48	181	4.28e-16	71.2
MS.gene033361.t1	MTR_5g027000	32.031	128	86	1	14	140	30	157	3.66e-15	68.6
MS.gene033361.t1	MTR_5g009830	36.220	127	76	2	15	139	380	503	4.89e-15	70.9
MS.gene033361.t1	MTR_5g008705	31.538	130	82	2	14	139	2	128	7.96e-15	66.6
MS.gene033361.t1	MTR_4g112460	29.927	137	93	1	6	139	11	147	1.08e-14	67.4
MS.gene033361.t1	MTR_3g088655	32.667	150	88	4	3	139	5	154	3.98e-14	65.5
MS.gene033361.t1	MTR_8g099095	33.858	127	79	2	15	139	380	503	6.59e-14	67.8
MS.gene033361.t1	MTR_5g092810	32.576	132	84	2	10	139	401	529	9.41e-14	67.4
MS.gene033361.t1	MTR_6g007613	31.111	135	92	1	6	139	29	163	1.30e-13	64.7
MS.gene033361.t1	MTR_1g026190	28.148	135	92	2	7	139	388	519	2.31e-13	66.2
MS.gene033361.t1	MTR_5g008695	33.043	115	66	2	31	138	2	112	3.54e-13	62.0
MS.gene033361.t1	MTR_0168s0120	33.043	115	67	3	31	139	1	111	3.88e-13	62.0
MS.gene033361.t1	MTR_6g025320	33.043	115	67	3	31	139	1	111	3.88e-13	62.0
MS.gene033361.t1	MTR_3g051770	30.882	136	88	2	10	139	392	527	4.13e-13	65.5
MS.gene033361.t1	MTR_4g132070	32.283	127	81	2	15	139	405	528	1.38e-12	63.9
MS.gene033361.t1	MTR_1g030440	30.345	145	84	3	6	139	22	160	1.42e-12	61.6
MS.gene033361.t1	MTR_7g074020	30.370	135	93	1	6	139	30	164	1.44e-12	61.6
MS.gene033361.t1	MTR_8g069915	26.277	137	98	1	6	139	12	148	4.41e-12	60.5
MS.gene033361.t1	MTR_5g089320	28.467	137	93	2	5	139	372	505	5.97e-12	62.0
MS.gene033361.t1	MTR_0028s0170	29.921	127	84	2	15	139	392	515	9.52e-12	61.6
MS.gene033361.t1	MTR_4g066660	31.387	137	87	3	7	140	378	510	1.31e-11	61.2
MS.gene033361.t1	MTR_7g115040	31.852	135	91	1	6	139	12	146	1.31e-11	58.9
MS.gene033361.t1	MTR_1g052530	31.746	126	86	0	15	140	366	491	2.33e-11	60.5
MS.gene033361.t1	MTR_7g068710	29.078	141	97	1	3	140	355	495	8.42e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene033361.t1	AT1G76650	47.794	136	71	0	4	139	41	176	5.23e-37	125
MS.gene033361.t1	AT1G76650	47.794	136	71	0	4	139	41	176	5.23e-37	125
MS.gene033361.t1	AT5G42380	47.794	136	69	1	6	139	49	184	4.75e-34	117
MS.gene033361.t1	AT1G76650	46.324	136	63	1	4	139	41	166	3.93e-33	114
MS.gene033361.t1	AT1G76640	44.604	139	74	1	4	139	20	158	1.73e-32	112
MS.gene033361.t1	AT3G50770	44.755	143	73	2	3	139	61	203	9.09e-32	112
MS.gene033361.t1	AT1G24620	45.185	135	73	1	6	139	37	171	7.72e-31	109
MS.gene033361.t1	AT3G01830	43.885	139	71	4	6	140	11	146	2.06e-28	102
MS.gene033361.t1	AT1G73630	36.496	137	84	1	3	139	17	150	5.37e-26	96.3
MS.gene033361.t1	AT1G18210	38.129	139	83	1	1	139	18	153	9.46e-26	95.9
MS.gene033361.t1	AT1G18210	38.129	139	83	1	1	139	18	153	9.46e-26	95.9
MS.gene033361.t1	AT5G37770	35.971	139	84	1	6	139	17	155	1.02e-25	95.5
MS.gene033361.t1	AT5G37770	35.971	139	84	1	6	139	17	155	1.02e-25	95.5
MS.gene033361.t1	AT1G66400	33.577	137	88	1	6	139	15	151	8.32e-24	90.5
MS.gene033361.t1	AT4G03290	39.007	141	80	2	6	140	5	145	5.21e-23	88.6
MS.gene033361.t1	AT1G05990	38.129	139	82	2	6	140	5	143	8.86e-22	85.1
MS.gene033361.t1	AT3G59440	35.714	140	88	1	3	140	48	187	1.83e-21	85.5
MS.gene033361.t1	AT4G12860	37.956	137	82	2	6	139	5	141	2.20e-21	84.3
MS.gene033361.t1	AT5G37780	36.429	140	79	3	6	139	12	147	3.78e-21	83.6
MS.gene033361.t1	AT1G66410	36.429	140	79	3	6	139	12	147	3.78e-21	83.6
MS.gene033361.t1	AT5G21274	36.429	140	79	3	6	139	12	147	3.91e-21	83.6
MS.gene033361.t1	AT3G56800	35.714	140	80	3	6	139	12	147	6.35e-21	83.2
MS.gene033361.t1	AT2G27030	35.714	140	80	3	6	139	12	147	6.35e-21	83.2
MS.gene033361.t1	AT2G41110	35.714	140	80	3	6	139	12	147	6.35e-21	83.2
MS.gene033361.t1	AT3G43810	35.714	140	80	3	6	139	12	147	8.96e-21	82.8
MS.gene033361.t1	AT2G43290	34.722	144	84	3	6	140	65	207	1.40e-20	84.0
MS.gene033361.t1	AT2G27030	35.714	140	80	3	6	139	12	147	1.46e-20	82.8
MS.gene033361.t1	AT3G22930	34.307	137	88	2	5	139	34	170	2.53e-20	82.0
MS.gene033361.t1	AT5G17470	35.294	136	81	2	7	139	7	138	3.13e-20	81.3
MS.gene033361.t1	AT3G43810	35.714	140	80	3	6	139	12	147	3.57e-20	82.8
MS.gene033361.t1	AT2G15680	37.778	135	81	2	6	139	50	182	4.12e-20	82.0
MS.gene033361.t1	AT3G07490	36.957	138	84	2	6	140	5	142	6.21e-20	80.5
MS.gene033361.t1	AT4G14640	34.532	139	82	2	6	139	13	147	1.29e-18	77.0
MS.gene033361.t1	AT2G36180	34.328	134	84	2	10	139	4	137	1.60e-18	76.6
MS.gene033361.t1	AT3G10190	32.143	140	93	2	3	140	67	206	2.47e-18	77.8
MS.gene033361.t1	AT2G41110	32.895	152	80	4	6	139	12	159	3.82e-18	76.3
MS.gene033361.t1	AT2G41100	28.947	152	90	1	6	139	66	217	1.86e-17	76.6
MS.gene033361.t1	AT2G41100	28.947	152	90	1	6	139	66	217	1.86e-17	76.6
MS.gene033361.t1	AT2G41100	28.947	152	90	1	6	139	66	217	1.86e-17	76.6
MS.gene033361.t1	AT2G41100	28.947	152	90	1	6	139	101	252	2.95e-17	76.6
MS.gene033361.t1	AT2G41100	27.152	151	93	1	6	139	12	162	3.46e-14	68.2
MS.gene033361.t1	AT2G41100	28.947	152	90	1	6	139	101	252	2.95e-17	76.6
MS.gene033361.t1	AT2G41100	27.152	151	93	1	6	139	12	162	3.46e-14	68.2
MS.gene033361.t1	AT5G37780	32.903	155	79	4	6	139	12	162	4.47e-17	73.6
MS.gene033361.t1	AT3G50360	31.852	135	91	1	6	139	27	161	1.21e-15	69.7
MS.gene033361.t1	AT4G23650	34.884	129	79	2	14	140	391	516	2.07e-15	72.0
MS.gene033361.t1	AT5G37780	30.723	166	79	4	6	139	12	173	2.43e-15	69.3
MS.gene033361.t1	AT3G51920	31.852	135	91	1	6	139	12	146	4.32e-15	68.2
MS.gene033361.t1	AT4G20780	30.769	156	87	3	5	139	28	183	6.47e-15	68.6
MS.gene033361.t1	AT3G03400	33.088	136	82	4	3	132	2	134	8.59e-15	67.0
MS.gene033361.t1	AT5G37780	33.858	127	72	3	20	139	13	134	8.86e-15	67.0
MS.gene033361.t1	AT1G66410	34.127	126	73	3	20	139	36	157	9.86e-15	67.4
MS.gene033361.t1	AT1G18530	29.197	137	94	1	6	139	7	143	1.22e-14	67.0
MS.gene033361.t1	AT4G37010	28.676	136	96	1	6	140	31	166	2.21e-14	66.6
MS.gene033361.t1	AT4G37010	28.676	136	96	1	6	140	27	162	2.75e-14	66.2
MS.gene033361.t1	AT4G04695	31.111	135	88	2	7	139	337	468	2.96e-14	68.9
MS.gene033361.t1	AT4G04700	31.852	135	87	2	7	139	337	468	6.61e-14	67.8
MS.gene033361.t1	AT2G41090	31.111	135	90	2	6	139	12	144	1.10e-13	65.1
MS.gene033361.t1	AT1G66410	33.913	115	66	3	31	139	1	111	1.75e-13	62.8
MS.gene033361.t1	AT1G66410	33.913	115	66	3	31	139	1	111	1.75e-13	62.8
MS.gene033361.t1	AT4G14640	32.800	125	75	2	20	139	9	129	2.52e-13	63.2
MS.gene033361.t1	AT1G62820	35.714	126	72	4	14	135	21	141	3.67e-13	62.8
MS.gene033361.t1	AT3G43810	33.043	115	67	3	31	139	1	111	4.06e-13	62.0
MS.gene033361.t1	AT3G43810	33.043	115	67	3	31	139	1	111	4.06e-13	62.0
MS.gene033361.t1	AT1G12310	35.714	126	72	4	14	135	21	141	5.27e-13	62.4
MS.gene033361.t1	AT3G51850	30.952	126	87	0	15	140	368	493	6.53e-13	65.1
MS.gene033361.t1	AT2G41410	31.884	138	90	3	6	139	70	207	6.53e-13	63.5
MS.gene033361.t1	AT1G32250	29.286	140	86	2	6	135	16	152	1.12e-12	62.0
MS.gene033361.t1	AT5G44460	29.530	149	92	3	5	140	27	175	1.14e-12	62.4
MS.gene033361.t1	AT1G76040	33.071	127	80	2	15	139	187	310	1.25e-12	63.9
MS.gene033361.t1	AT1G76040	33.071	127	80	2	15	139	187	310	1.25e-12	63.9
MS.gene033361.t1	AT2G31500	34.014	147	85	5	3	140	365	508	1.47e-12	63.9
MS.gene033361.t1	AT4G04720	31.061	132	86	2	10	139	388	516	1.59e-12	63.9
MS.gene033361.t1	AT4G04720	31.061	132	86	2	10	139	388	516	1.59e-12	63.9
MS.gene033361.t1	AT1G76040	33.071	127	80	2	15	139	425	548	1.73e-12	63.9
MS.gene033361.t1	AT1G50700	30.303	132	87	2	10	139	382	510	1.78e-12	63.5
MS.gene033361.t1	AT5G19360	32.283	127	81	2	15	139	382	505	2.27e-12	63.5
MS.gene033361.t1	AT3G01830	41.509	106	55	4	6	107	11	113	2.32e-12	60.5
MS.gene033361.t1	AT3G25600	27.737	137	96	1	6	139	12	148	2.35e-12	61.2
MS.gene033361.t1	AT1G74740	29.545	132	91	2	10	140	368	498	3.39e-12	62.8
MS.gene033361.t1	AT3G20410	31.061	132	86	2	10	139	400	528	4.03e-12	62.8
MS.gene033361.t1	AT3G20410	31.061	132	86	2	10	139	400	528	4.03e-12	62.8
MS.gene033361.t1	AT3G03000	30.303	132	90	1	6	135	20	151	4.22e-12	60.5
MS.gene033361.t1	AT4G21940	32.283	127	81	2	15	139	415	538	4.29e-12	62.8
MS.gene033361.t1	AT4G21940	32.283	127	81	2	15	139	415	538	4.34e-12	62.8
MS.gene033361.t1	AT5G12180	31.496	127	82	2	15	139	387	510	1.49e-11	61.2
MS.gene033361.t1	AT5G12480	31.496	127	86	1	15	140	279	405	3.74e-11	59.7
MS.gene033361.t1	AT2G41100	29.630	108	58	1	50	139	20	127	6.82e-11	57.8
MS.gene033361.t1	AT1G61950	30.075	133	88	2	10	140	408	537	7.11e-11	58.9

Find 35 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGGATTTAATAACGCTTA+TGG	0.268235	8.4:+21979736	MS.gene033361:CDS
AACATCCTCTTCAAACTCTT+TGG	0.271094	8.4:-21979842	None:intergenic
CATCACTTTACATTCTTCAA+TGG	0.327118	8.4:-21979888	None:intergenic
GATGATTGGAAGGTTTGATT+TGG	0.354750	8.4:+21979907	MS.gene033361:CDS
TTAATAACGCTTATGGAATC+CGG	0.394530	8.4:+21979743	MS.gene033361:CDS
AGATTTCACCGAACGAGTTA+AGG	0.395728	8.4:+21979618	MS.gene033361:CDS
AAGAAAAGTTGAAGGATTTG+AGG	0.418092	8.4:+21979774	MS.gene033361:CDS
GGAAATTGCTATTGAGGCAT+TGG	0.422915	8.4:+21979685	MS.gene033361:CDS
TGGTGATGGGCTATTGAGTT+TGG	0.423743	8.4:+21979715	MS.gene033361:CDS
AGAAAAGTTGAAGGATTTGA+GGG	0.426919	8.4:+21979775	MS.gene033361:CDS
AGGAGTCGAATGACGAAGAT+TGG	0.473202	8.4:+21979638	MS.gene033361:CDS
ATTCGACTCCTTAACTCGTT+CGG	0.474759	8.4:-21979626	None:intergenic
GAAGAATGTAAAGTGATGAT+TGG	0.486801	8.4:+21979893	MS.gene033361:CDS
TAATAACGCTTATGGAATCC+GGG	0.494077	8.4:+21979744	MS.gene033361:CDS
AGAAGTGGAAATTGCTATTG+AGG	0.499700	8.4:+21979679	MS.gene033361:CDS
AATGTAAAGTGATGATTGGA+AGG	0.500397	8.4:+21979897	MS.gene033361:CDS
AGAGTTTCAATTGAAAGAAG+TGG	0.502556	8.4:+21979664	MS.gene033361:CDS
TGAAGAATTCAGAATCATGA+TGG	0.506151	8.4:+21979952	MS.gene033361:CDS
AGGTTTGATTTGGATGGTGA+TGG	0.507936	8.4:+21979917	MS.gene033361:CDS
ATTGGAAGGTTTGATTTGGA+TGG	0.510943	8.4:+21979911	MS.gene033361:CDS
TGATGGGCTATTGAGTTTGG+AGG	0.520299	8.4:+21979718	MS.gene033361:CDS
TTTGAAATGTATGATAATGA+AGG	0.534288	8.4:+21979803	MS.gene033361:CDS
ATGTATGATAATGAAGGATG+TGG	0.537649	8.4:+21979809	MS.gene033361:CDS
GCATTGGATTCTGATGGTGA+TGG	0.540839	8.4:+21979701	MS.gene033361:CDS
TACTTTGATGAAGACGGTGA+TGG	0.542295	8.4:+21979593	MS.gene033361:CDS
GGGAGAAGAAGAAAAGTTGA+AGG	0.545380	8.4:+21979766	MS.gene033361:CDS
CTAAGTTACTTTGATGAAGA+CGG	0.574980	8.4:+21979587	MS.gene033361:CDS
AGTCGAATGACGAAGATTGG+CGG	0.582253	8.4:+21979641	MS.gene033361:CDS
CATTGGATTCTGATGGTGAT+GGG	0.600002	8.4:+21979702	MS.gene033361:CDS
TAACACCAAAGAGTTTGAAG+AGG	0.608054	8.4:+21979837	MS.gene033361:CDS
AATTAACATGAGGAGTAACA+TGG	0.611175	8.4:+21979550	None:intergenic
ATTGAGGCATTGGATTCTGA+TGG	0.616237	8.4:+21979695	MS.gene033361:CDS
TGTATGATAATGAAGGATGT+GGG	0.638642	8.4:+21979810	MS.gene033361:CDS
AATAACGCTTATGGAATCCG+GGG	0.638897	8.4:+21979745	MS.gene033361:CDS
ATAACGCTTATGGAATCCGG+GGG	0.690847	8.4:+21979746	MS.gene033361:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGAAATGTATGATAATGA+AGG	+	chr8.4:21979803-21979822	MS.gene033361:CDS	20.0%
	AAGAAAAGTTGAAGGATTTG+AGG	+	chr8.4:21979774-21979793	MS.gene033361:CDS	30.0%
	AAGAGGATGTTGAAAAAGTT+GGG	+	chr8.4:21979854-21979873	MS.gene033361:CDS	30.0%
	AATGTAAAGTGATGATTGGA+AGG	+	chr8.4:21979897-21979916	MS.gene033361:CDS	30.0%
	AGAAAAGTTGAAGGATTTGA+GGG	+	chr8.4:21979775-21979794	MS.gene033361:CDS	30.0%
	AGAGTTTCAATTGAAAGAAG+TGG	+	chr8.4:21979664-21979683	MS.gene033361:CDS	30.0%
	CATCACTTTACATTCTTCAA+TGG	-	chr8.4:21979891-21979910	None:intergenic	30.0%
	GAAGAATGTAAAGTGATGAT+TGG	+	chr8.4:21979893-21979912	MS.gene033361:CDS	30.0%
	TGAAGAATTCAGAATCATGA+TGG	+	chr8.4:21979952-21979971	MS.gene033361:CDS	30.0%
	TTAATAACGCTTATGGAATC+CGG	+	chr8.4:21979743-21979762	MS.gene033361:CDS	30.0%
!	ATGTATGATAATGAAGGATG+TGG	+	chr8.4:21979809-21979828	MS.gene033361:CDS	30.0%
!	CTAAGTTACTTTGATGAAGA+CGG	+	chr8.4:21979587-21979606	MS.gene033361:CDS	30.0%
!	TGTATGATAATGAAGGATGT+GGG	+	chr8.4:21979810-21979829	MS.gene033361:CDS	30.0%
	AACATCCTCTTCAAACTCTT+TGG	-	chr8.4:21979845-21979864	None:intergenic	35.0%
	AGAGGATGTTGAAAAAGTTG+GGG	+	chr8.4:21979855-21979874	MS.gene033361:CDS	35.0%
	GAAGAGGATGTTGAAAAAGT+TGG	+	chr8.4:21979853-21979872	MS.gene033361:CDS	35.0%
	GATGATTGGAAGGTTTGATT+TGG	+	chr8.4:21979907-21979926	MS.gene033361:CDS	35.0%
	GGAGGATTTAATAACGCTTA+TGG	+	chr8.4:21979736-21979755	MS.gene033361:CDS	35.0%
	TAACACCAAAGAGTTTGAAG+AGG	+	chr8.4:21979837-21979856	MS.gene033361:CDS	35.0%
	TAATAACGCTTATGGAATCC+GGG	+	chr8.4:21979744-21979763	MS.gene033361:CDS	35.0%
!	AGAAGTGGAAATTGCTATTG+AGG	+	chr8.4:21979679-21979698	MS.gene033361:CDS	35.0%
!!	ATTGGAAGGTTTGATTTGGA+TGG	+	chr8.4:21979911-21979930	MS.gene033361:CDS	35.0%
	AATAACGCTTATGGAATCCG+GGG	+	chr8.4:21979745-21979764	MS.gene033361:CDS	40.0%
	AGATTTCACCGAACGAGTTA+AGG	+	chr8.4:21979618-21979637	MS.gene033361:CDS	40.0%
	ATTCGACTCCTTAACTCGTT+CGG	-	chr8.4:21979629-21979648	None:intergenic	40.0%
	GAGGATGTTGAAAAAGTTGG+GGG	+	chr8.4:21979856-21979875	MS.gene033361:CDS	40.0%
	GGGAGAAGAAGAAAAGTTGA+AGG	+	chr8.4:21979766-21979785	MS.gene033361:CDS	40.0%
	TACTTTGATGAAGACGGTGA+TGG	+	chr8.4:21979593-21979612	MS.gene033361:CDS	40.0%
!	CATTGGATTCTGATGGTGAT+GGG	+	chr8.4:21979702-21979721	MS.gene033361:CDS	40.0%
!	GGAAATTGCTATTGAGGCAT+TGG	+	chr8.4:21979685-21979704	MS.gene033361:CDS	40.0%
!!	AGGTTTGATTTGGATGGTGA+TGG	+	chr8.4:21979917-21979936	MS.gene033361:CDS	40.0%
!!	ATTGAGGCATTGGATTCTGA+TGG	+	chr8.4:21979695-21979714	MS.gene033361:CDS	40.0%
	AGGAGTCGAATGACGAAGAT+TGG	+	chr8.4:21979638-21979657	MS.gene033361:CDS	45.0%
	AGTCGAATGACGAAGATTGG+CGG	+	chr8.4:21979641-21979660	MS.gene033361:CDS	45.0%
	ATAACGCTTATGGAATCCGG+GGG	+	chr8.4:21979746-21979765	MS.gene033361:CDS	45.0%
!	CAACTTTTCTTCTTCTCCCC+CGG	-	chr8.4:21979765-21979784	None:intergenic	45.0%
!	GCATTGGATTCTGATGGTGA+TGG	+	chr8.4:21979701-21979720	MS.gene033361:CDS	45.0%
!!	TGATGGGCTATTGAGTTTGG+AGG	+	chr8.4:21979718-21979737	MS.gene033361:CDS	45.0%
!!	TGGTGATGGGCTATTGAGTT+TGG	+	chr8.4:21979715-21979734	MS.gene033361:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	21979557	21979979	21979557	ID=MS.gene033361
chr8.4	mRNA	21979557	21979979	21979557	ID=MS.gene033361.t1;Parent=MS.gene033361
chr8.4	exon	21979557	21979979	21979557	ID=MS.gene033361.t1.exon1;Parent=MS.gene033361.t1
chr8.4	CDS	21979557	21979979	21979557	ID=cds.MS.gene033361.t1;Parent=MS.gene033361.t1

Gene Sequence

>MS.gene033361

ATGAGGAGTAACATGGAATTTGAGCGTGTACTAAGTTACTTTGATGAAGACGGTGATGGAAAGATTTCACCGAACGAGTTAAGGAGTCGAATGACGAAGATTGGCGGAGAGTTTCAATTGAAAGAAGTGGAAATTGCTATTGAGGCATTGGATTCTGATGGTGATGGGCTATTGAGTTTGGAGGATTTAATAACGCTTATGGAATCCGGGGGAGAAGAAGAAAAGTTGAAGGATTTGAGGGAAGCTTTTGAAATGTATGATAATGAAGGATGTGGGTTCATAACACCAAAGAGTTTGAAGAGGATGTTGAAAAAGTTGGGGGATTCTAAGTCCATTGAAGAATGTAAAGTGATGATTGGAAGGTTTGATTTGGATGGTGATGGTGTGCTTAGTTTTGAAGAATTCAGAATCATGATGGAGTGA

Protein sequence

>MS.gene033361.t1

MRSNMEFERVLSYFDEDGDGKISPNELRSRMTKIGGEFQLKEVEIAIEALDSDGDGLLSLEDLITLMESGGEEEKLKDLREAFEMYDNEGCGFITPKSLKRMLKKLGDSKSIEECKVMIGRFDLDGDGVLSFEEFRIMME