AlfalfaGEDB | MS.gene057806

M. sativa cultivar XinJiangDaYe / MS.gene057806

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057806.t1	AES89321.1	95.3	86	4	0	1	86	1	86	6.30E-40	172.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057806.t1	P33080	70.0	90	23	1	1	86	1	90	1.6e-31	136.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057806.t1	G7JL46	95.3	86	4	0	1	86	1	86	4.5e-40	172.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056191	MS.gene057806	0.805403	1.36E-49	-1.69E-46
MS.gene057782	MS.gene057806	0.805095	1.58E-49	-1.69E-46
MS.gene057806	MS.gene062971	0.813834	2.09E-51	-1.69E-46
MS.gene057806	MS.gene27514	0.803031	4.23E-49	-1.69E-46
MS.gene057806	MS.gene88768	0.801652	8.14E-49	-1.69E-46
MS.gene057806	MS.gene92773	0.805811	1.11E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene057806	MS.gene002380	PPI
MS.gene062979	MS.gene057806	PPI
MS.gene057806	MS.gene68165	PPI
MS.gene01358	MS.gene057806	PPI
MS.gene057806	MS.gene50677	PPI
MS.gene057806	MS.gene50684	PPI
MS.gene057806	MS.gene045366	PPI
MS.gene057790	MS.gene057806	PPI
MS.gene40326	MS.gene057806	PPI
MS.gene062952	MS.gene057806	PPI
MS.gene057806	MS.gene045362	PPI
MS.gene057806	MS.gene23372	PPI
MS.gene057806	MS.gene014668	PPI
MS.gene057806	MS.gene50765	PPI
MS.gene057806	MS.gene68164	PPI
MS.gene50676	MS.gene057806	PPI
MS.gene057806	MS.gene68759	PPI
MS.gene057806	MS.gene003586	PPI
MS.gene23370	MS.gene057806	PPI
MS.gene77529	MS.gene057806	PPI
MS.gene014667	MS.gene057806	PPI
MS.gene057806	MS.gene003627	PPI
MS.gene003594	MS.gene057806	PPI
MS.gene50665	MS.gene057806	PPI
MS.gene057806	MS.gene79891	PPI
MS.gene057778	MS.gene057806	PPI
MS.gene057806	MS.gene045364	PPI
MS.gene057806	MS.gene50687	PPI
MS.gene50763	MS.gene057806	PPI
MS.gene057806	MS.gene014669	PPI
MS.gene057806	MS.gene057808	PPI
MS.gene045365	MS.gene057806	PPI
MS.gene50659	MS.gene057806	PPI
MS.gene75316	MS.gene057806	PPI
MS.gene057806	MS.gene77526	PPI
MS.gene057806	MS.gene68163	PPI
MS.gene003624	MS.gene057806	PPI
MS.gene057806	MS.gene75315	PPI
MS.gene031201	MS.gene057806	PPI
MS.gene057806	MS.gene057818	PPI
MS.gene057806	MS.gene79889	PPI
MS.gene057806	MS.gene045368	PPI
MS.gene057806	MS.gene031200	PPI
MS.gene057806	MS.gene045360	PPI
MS.gene057806	MS.gene014222	PPI
MS.gene057806	MS.gene79888	PPI
MS.gene057806	MS.gene75676	PPI
MS.gene057806	MS.gene75678	PPI
MS.gene77523	MS.gene057806	PPI
MS.gene057806	MS.gene031199	PPI
MS.gene057806	MS.gene014223	PPI
MS.gene057806	MS.gene75673	PPI
MS.gene057806	MS.gene79885	PPI
MS.gene79890	MS.gene057806	PPI
MS.gene003615	MS.gene057806	PPI
MS.gene062973	MS.gene057806	PPI
MS.gene057806	MS.gene50764	PPI
MS.gene003602	MS.gene057806	PPI
MS.gene057806	MS.gene062963	PPI
MS.gene047517	MS.gene057806	PPI
MS.gene057806	MS.gene003577	PPI
MS.gene062984	MS.gene057806	PPI
MS.gene057806	MS.gene50675	PPI
MS.gene40327	MS.gene057806	PPI
MS.gene057806	MS.gene014670	PPI
MS.gene057806	MS.gene75314	PPI
MS.gene057806	MS.gene01764	PPI
MS.gene003591	MS.gene057806	PPI
MS.gene003587	MS.gene057806	PPI
MS.gene057806	MS.gene75679	PPI
MS.gene057806	MS.gene40325	PPI
MS.gene003590	MS.gene057806	PPI
MS.gene062943	MS.gene057806	PPI
MS.gene063112	MS.gene057806	PPI
MS.gene003608	MS.gene057806	PPI
MS.gene057806	MS.gene68760	PPI
MS.gene057806	MS.gene031198	PPI
MS.gene057806	MS.gene057797	PPI
MS.gene057806	MS.gene003614	PPI
MS.gene057806	MS.gene77528	PPI
MS.gene057806	MS.gene79886	PPI
MS.gene057806	MS.gene014671	PPI
MS.gene057806	MS.gene003596	PPI
MS.gene057806	MS.gene062988	PPI
MS.gene057806	MS.gene23371	PPI
MS.gene057806	MS.gene75672	PPI
MS.gene50766	MS.gene057806	PPI
MS.gene057806	MS.gene75674	PPI
MS.gene057806	MS.gene50654	PPI
MS.gene057806	MS.gene062974	PPI
MS.gene75677	MS.gene057806	PPI
MS.gene003625	MS.gene057806	PPI
MS.gene057806	MS.gene003583	PPI
MS.gene057806	MS.gene062951	PPI
MS.gene057806	MS.gene057801	PPI
MS.gene057806	MS.gene77527	PPI
MS.gene057806	MS.gene77524	PPI
MS.gene057806	MS.gene057795	PPI
MS.gene057806	MS.gene68162	PPI
MS.gene057806	MS.gene045367	PPI
MS.gene057806	MS.gene045363	PPI
MS.gene057806	MS.gene75675	PPI
MS.gene045361	MS.gene057806	PPI
MS.gene057806	MS.gene79887	PPI
MS.gene057806	MS.gene50652	PPI
MS.gene057806	MS.gene68761	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057806.t1	MTR_4g072630	95.349	86	4	0	1	86	1	86	1.07e-58	174
MS.gene057806.t1	MTR_4g072465	89.535	86	9	0	1	86	1	86	5.06e-55	165
MS.gene057806.t1	MTR_4g072490	89.535	86	8	1	1	86	1	85	4.88e-54	162
MS.gene057806.t1	MTR_4g072740	88.372	86	9	1	1	86	1	85	3.22e-53	160
MS.gene057806.t1	MTR_4g072510	95.349	86	4	0	1	86	1	86	1.25e-50	154
MS.gene057806.t1	MTR_4g072670	93.023	86	6	0	1	86	1	86	1.06e-49	151
MS.gene057806.t1	MTR_4g072580	84.884	86	12	1	1	86	1	85	1.21e-49	151
MS.gene057806.t1	MTR_4g072370	82.955	88	13	1	1	86	1	88	1.29e-49	151
MS.gene057806.t1	MTR_4g072540	93.023	86	6	0	1	86	1	86	3.22e-49	150
MS.gene057806.t1	MTR_4g072470	83.721	86	13	1	1	86	1	85	1.01e-48	149
MS.gene057806.t1	MTR_4g072830	82.759	87	13	1	1	85	1	87	1.35e-48	149
MS.gene057806.t1	MTR_4g072720	90.698	86	8	0	1	86	1	86	7.11e-48	147
MS.gene057806.t1	MTR_4g072600	81.395	86	15	1	1	86	1	85	6.95e-47	144
MS.gene057806.t1	MTR_4g072610	80.233	86	15	1	1	86	1	84	1.43e-45	141
MS.gene057806.t1	MTR_4g072650	79.070	86	16	1	1	86	1	84	8.27e-45	139
MS.gene057806.t1	MTR_4g072330	76.404	89	17	2	1	86	1	88	9.73e-45	139
MS.gene057806.t1	MTR_3g084170	73.333	90	20	1	1	86	1	90	1.23e-44	139
MS.gene057806.t1	MTR_4g072870	72.826	92	18	2	1	86	1	91	2.18e-44	138
MS.gene057806.t1	MTR_3g084150	72.222	90	21	1	1	86	1	90	5.78e-44	137
MS.gene057806.t1	MTR_3g084240	72.222	90	21	1	1	86	1	90	1.57e-43	136
MS.gene057806.t1	MTR_5g008350	84.000	75	11	1	1	75	1	74	2.93e-43	135
MS.gene057806.t1	MTR_3g084220	71.111	90	22	1	1	86	1	90	3.57e-43	135
MS.gene057806.t1	MTR_3g084180	71.111	90	22	1	1	86	1	90	4.97e-43	135
MS.gene057806.t1	MTR_3g110038	71.111	90	22	1	1	86	1	90	1.07e-42	134
MS.gene057806.t1	MTR_4g072690	81.395	86	15	1	1	86	1	85	1.17e-42	134
MS.gene057806.t1	MTR_8g461410	70.000	90	23	1	1	86	1	90	1.64e-42	134
MS.gene057806.t1	MTR_4g072240	68.132	91	24	1	1	86	1	91	6.79e-42	132
MS.gene057806.t1	MTR_3g110032	69.231	91	23	1	1	86	1	91	1.09e-41	131
MS.gene057806.t1	MTR_4g072590	79.070	86	17	1	1	86	1	85	2.19e-41	130
MS.gene057806.t1	MTR_4g050700	69.231	91	23	1	1	86	1	91	4.91e-41	130
MS.gene057806.t1	MTR_2g012410	69.231	91	23	1	1	86	1	91	4.91e-41	130
MS.gene057806.t1	MTR_4g072300	65.934	91	26	1	1	86	1	91	7.84e-41	129
MS.gene057806.t1	MTR_4g072450	79.070	86	17	1	1	86	1	85	1.11e-40	129
MS.gene057806.t1	MTR_4g072220	67.033	91	25	1	1	86	1	91	1.16e-40	129
MS.gene057806.t1	MTR_4g072880	68.132	91	24	1	1	86	1	91	1.66e-40	128
MS.gene057806.t1	MTR_4g072890	65.934	91	26	1	1	86	1	91	1.82e-40	128
MS.gene057806.t1	MTR_4g072310	68.132	91	24	1	1	86	1	91	2.09e-40	128
MS.gene057806.t1	MTR_4g072480	66.667	90	26	1	1	86	1	90	2.55e-40	128
MS.gene057806.t1	MTR_4g072380	73.563	87	21	2	1	86	1	86	6.61e-40	127
MS.gene057806.t1	MTR_4g072260	64.835	91	27	1	1	86	1	91	7.63e-40	127
MS.gene057806.t1	MTR_3g110042	69.663	89	23	1	1	85	1	89	1.21e-39	126
MS.gene057806.t1	MTR_4g072250	71.429	91	20	2	1	86	1	90	1.61e-39	126
MS.gene057806.t1	MTR_4g072820	74.713	87	19	2	1	86	1	85	2.39e-39	125
MS.gene057806.t1	MTR_3g084260	67.033	91	25	1	1	86	1	91	2.63e-39	125
MS.gene057806.t1	MTR_4g072730	64.444	90	28	1	1	86	1	90	6.02e-39	124
MS.gene057806.t1	MTR_4g072620	63.333	90	29	1	1	86	1	90	6.36e-39	124
MS.gene057806.t1	MTR_4g072680	64.444	90	28	1	1	86	1	90	1.38e-38	124
MS.gene057806.t1	MTR_4g072570	66.667	90	26	1	1	86	1	90	1.53e-38	124
MS.gene057806.t1	MTR_3g084250	66.667	90	26	1	1	86	1	90	1.84e-38	123
MS.gene057806.t1	MTR_4g072850	68.478	92	23	1	1	86	1	92	2.00e-38	123
MS.gene057806.t1	MTR_4g072270	64.835	91	27	1	1	86	1	91	2.09e-38	123
MS.gene057806.t1	MTR_4g072500	67.778	90	25	1	1	86	1	90	2.56e-38	123
MS.gene057806.t1	MTR_4g072860	70.652	92	20	2	1	86	1	91	3.87e-38	122
MS.gene057806.t1	MTR_4g072530	66.667	90	26	1	1	86	1	90	1.05e-37	121
MS.gene057806.t1	MTR_4g072660	65.116	86	29	1	1	86	1	85	2.36e-37	120
MS.gene057806.t1	MTR_4g072640	67.033	91	24	2	1	86	1	90	2.83e-37	120
MS.gene057806.t1	MTR_4g072460	62.222	90	30	1	1	86	1	90	4.68e-37	120
MS.gene057806.t1	MTR_3g117630	73.973	73	19	0	14	86	18	90	6.80e-37	119
MS.gene057806.t1	MTR_3g084210	65.556	90	27	1	1	86	1	90	4.06e-36	117
MS.gene057806.t1	MTR_4g072230	65.934	91	24	2	1	86	1	89	5.02e-36	117
MS.gene057806.t1	MTR_8g461420	67.778	90	25	1	1	86	1	90	5.23e-36	117
MS.gene057806.t1	MTR_3g084190	66.667	90	26	1	1	86	1	90	5.77e-36	117
MS.gene057806.t1	MTR_3g084230	66.667	90	26	1	1	86	1	90	2.99e-35	115
MS.gene057806.t1	MTR_3g110045	68.182	88	24	1	3	86	62	149	4.06e-35	117
MS.gene057806.t1	MTR_3g084160	65.556	90	27	1	1	86	1	90	8.66e-35	114
MS.gene057806.t1	MTR_5g008370	65.556	90	27	1	1	86	1	90	1.59e-34	115
MS.gene057806.t1	MTR_5g008360	66.667	78	26	0	9	86	4	81	1.79e-34	113
MS.gene057806.t1	MTR_4g072940	64.444	90	26	1	3	86	33	122	3.59e-33	111
MS.gene057806.t1	MTR_3g084200	69.444	72	18	1	1	68	1	72	1.11e-31	106
MS.gene057806.t1	MTR_4g072560	53.608	97	33	3	1	85	1	97	8.02e-31	104
MS.gene057806.t1	MTR_4g072770	60.465	86	28	1	1	86	1	80	1.81e-30	103
MS.gene057806.t1	MTR_4g072910	63.636	77	27	1	10	86	25	100	4.79e-30	102
MS.gene057806.t1	MTR_4g072550	66.197	71	24	0	16	86	31	101	5.42e-30	102
MS.gene057806.t1	MTR_4g072980	53.261	92	36	2	1	85	1	92	2.25e-29	100
MS.gene057806.t1	MTR_3g117640	66.176	68	22	1	20	86	33	100	3.01e-29	100
MS.gene057806.t1	MTR_4g072290	58.140	86	27	2	1	86	1	77	1.08e-26	93.6
MS.gene057806.t1	MTR_4g072280	60.000	75	25	1	1	70	1	75	2.31e-26	92.8
MS.gene057806.t1	MTR_4g072750	64.865	74	20	2	1	68	1	74	4.28e-25	89.7
MS.gene057806.t1	MTR_3g110035	62.500	64	24	0	23	86	73	136	7.01e-25	90.5
MS.gene057806.t1	MTR_3g117610	60.000	65	25	1	19	83	43	106	2.92e-23	85.9
MS.gene057806.t1	MTR_3g113310	55.224	67	29	1	19	85	40	105	1.48e-22	84.0
MS.gene057806.t1	MTR_6g077850	53.731	67	31	0	20	86	33	99	2.35e-22	83.2
MS.gene057806.t1	MTR_4g072190	52.055	73	34	1	11	83	26	97	3.97e-22	82.4
MS.gene057806.t1	MTR_4g072930	50.617	81	35	2	11	86	30	110	1.74e-21	81.3
MS.gene057806.t1	MTR_8g461400	47.778	90	17	2	1	86	1	64	3.62e-20	76.6
MS.gene057806.t1	MTR_5g021820	46.429	84	42	2	4	85	21	103	1.08e-19	76.6
MS.gene057806.t1	MTR_8g076040	54.688	64	28	1	20	83	40	102	2.04e-18	73.2
MS.gene057806.t1	MTR_4g072840	49.425	87	16	3	1	86	1	60	2.24e-18	72.0
MS.gene057806.t1	MTR_2g043960	57.627	59	24	1	20	78	75	132	7.49e-18	73.2
MS.gene057806.t1	MTR_4g124750	55.932	59	25	1	20	78	68	125	1.77e-17	72.0
MS.gene057806.t1	MTR_1g061570	48.101	79	38	2	5	81	1	78	7.32e-16	67.4
MS.gene057806.t1	MTR_1g094750	51.613	62	29	1	20	81	49	109	8.47e-16	67.0
MS.gene057806.t1	MTR_1g009150	50.725	69	33	1	13	81	63	130	1.21e-15	68.2
MS.gene057806.t1	MTR_5g008380	90.625	32	3	0	55	86	246	277	4.37e-15	68.2
MS.gene057806.t1	MTR_4g005310	48.387	62	31	1	21	81	62	123	5.74e-15	65.9
MS.gene057806.t1	MTR_1g063950	46.774	62	32	1	20	81	47	107	7.82e-15	64.7
MS.gene057806.t1	MTR_7g101740	50.000	64	31	1	20	83	39	101	8.34e-15	64.3
MS.gene057806.t1	MTR_7g118010	49.231	65	30	2	21	83	23	86	1.30e-14	63.5
MS.gene057806.t1	MTR_4g025830	43.662	71	36	2	18	85	76	145	4.76e-14	63.5
MS.gene057806.t1	MTR_4g005320	49.180	61	30	1	21	81	70	129	4.88e-14	63.5
MS.gene057806.t1	MTR_3g105900	66.667	42	14	0	20	61	1171	1212	5.38e-14	65.9
MS.gene057806.t1	MTR_1g024085	52.542	59	27	1	20	78	36	93	6.08e-14	62.8
MS.gene057806.t1	MTR_0388s0020	41.026	78	42	2	11	85	65	141	9.18e-14	62.4
MS.gene057806.t1	MTR_3g092220	44.928	69	35	2	12	78	24	91	1.04e-13	61.6
MS.gene057806.t1	MTR_8g461210	43.421	76	38	2	7	78	17	91	1.89e-13	60.8
MS.gene057806.t1	MTR_4g072160	45.333	75	39	2	8	81	23	96	5.73e-13	60.1
MS.gene057806.t1	MTR_3g109160	50.820	61	29	1	21	81	69	128	6.20e-13	60.8
MS.gene057806.t1	MTR_8g026730	44.444	63	32	2	21	81	27	88	2.87e-12	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057806.t1	AT4G38840	73.529	68	17	1	20	86	32	99	1.27e-33	111
MS.gene057806.t1	AT4G34800	58.333	84	32	1	1	81	1	84	1.18e-30	103
MS.gene057806.t1	AT2G21210	55.556	81	34	1	8	86	15	95	1.32e-30	103
MS.gene057806.t1	AT2G21210	55.556	81	34	1	8	86	82	162	7.44e-30	103
MS.gene057806.t1	AT5G18080	59.459	74	29	1	14	86	17	90	2.05e-29	100
MS.gene057806.t1	AT4G34810	61.538	78	26	1	10	83	37	114	6.50e-29	100
MS.gene057806.t1	AT5G18020	58.108	74	30	1	14	86	17	90	1.69e-28	98.6
MS.gene057806.t1	AT4G38850	56.098	82	28	2	8	86	13	89	1.88e-28	98.2
MS.gene057806.t1	AT5G18050	57.692	78	29	2	10	86	16	90	2.31e-28	98.2
MS.gene057806.t1	AT4G38825	57.692	78	27	2	10	86	17	89	2.32e-28	98.2
MS.gene057806.t1	AT5G18060	63.636	66	23	1	22	86	25	90	2.81e-28	97.8
MS.gene057806.t1	AT5G18030	56.962	79	30	2	9	86	13	88	3.57e-28	97.4
MS.gene057806.t1	AT2G21200	59.722	72	28	1	15	85	15	86	4.80e-28	97.1
MS.gene057806.t1	AT5G18010	56.757	74	31	1	14	86	17	90	1.12e-27	96.3
MS.gene057806.t1	AT4G34770	58.333	84	33	1	5	86	19	102	8.45e-27	94.7
MS.gene057806.t1	AT3G03820	55.696	79	32	1	11	86	17	95	2.71e-26	93.2
MS.gene057806.t1	AT4G13790	49.412	85	40	2	3	85	8	91	8.74e-26	91.7
MS.gene057806.t1	AT3G03840	53.846	78	34	1	11	86	17	94	1.89e-25	90.9
MS.gene057806.t1	AT4G34790	68.657	67	18	2	21	84	39	105	2.40e-25	90.9
MS.gene057806.t1	AT3G03850	61.194	67	24	1	22	86	26	92	4.25e-25	89.7
MS.gene057806.t1	AT3G03830	55.696	79	29	2	11	86	17	92	3.82e-24	87.4
MS.gene057806.t1	AT4G38860	49.412	85	41	1	4	86	21	105	8.81e-24	87.0
MS.gene057806.t1	AT2G21220	48.780	82	39	2	4	83	21	101	4.48e-22	82.4
MS.gene057806.t1	AT4G34780	57.143	70	27	1	19	85	26	95	7.80e-22	82.0
MS.gene057806.t1	AT1G75580	58.209	67	27	1	19	85	42	107	1.25e-21	81.6
MS.gene057806.t1	AT4G34760	56.923	65	27	1	19	83	41	104	5.30e-21	80.1
MS.gene057806.t1	AT2G16580	56.923	65	27	1	19	83	42	105	1.36e-20	79.0
MS.gene057806.t1	AT4G36110	53.125	64	29	1	20	83	39	101	7.81e-19	74.3
MS.gene057806.t1	AT1G19830	58.824	68	26	2	19	85	46	112	1.38e-18	73.9
MS.gene057806.t1	AT2G18010	53.125	64	29	1	20	83	47	109	2.06e-18	73.6
MS.gene057806.t1	AT3G43120	55.932	59	25	1	20	78	78	135	3.50e-18	74.3
MS.gene057806.t1	AT5G20810	57.627	59	24	1	20	78	78	135	2.18e-17	72.4
MS.gene057806.t1	AT5G20810	57.627	59	24	1	20	78	78	135	3.01e-17	72.4
MS.gene057806.t1	AT3G20220	50.000	62	29	2	21	81	47	107	2.61e-15	65.9
MS.gene057806.t1	AT5G66260	50.000	66	32	1	21	86	34	98	5.69e-15	64.3
MS.gene057806.t1	AT2G37030	50.794	63	30	1	20	82	48	109	1.24e-14	64.3
MS.gene057806.t1	AT1G75590	49.153	59	29	1	20	78	41	98	1.60e-13	62.0
MS.gene057806.t1	AT3G51200	43.902	82	40	3	5	81	19	99	1.64e-13	60.8
MS.gene057806.t1	AT3G61900	40.506	79	42	3	12	86	20	97	2.29e-13	61.2
MS.gene057806.t1	AT5G10990	44.000	75	36	2	4	78	29	97	3.28e-13	61.2
MS.gene057806.t1	AT2G46690	42.857	77	40	2	5	78	8	83	3.64e-13	60.5
MS.gene057806.t1	AT3G53250	46.032	63	30	1	20	82	51	109	4.62e-13	60.1
MS.gene057806.t1	AT4G34750	43.750	64	35	1	20	83	36	98	5.01e-13	60.5
MS.gene057806.t1	AT4G34750	43.750	64	35	1	20	83	36	98	5.01e-13	60.5
MS.gene057806.t1	AT3G53250	46.032	63	30	1	20	82	82	140	8.71e-13	60.1
MS.gene057806.t1	AT1G19840	45.333	75	35	2	4	78	29	97	9.54e-13	60.1
MS.gene057806.t1	AT4G31320	47.541	61	31	1	21	81	80	139	3.25e-12	59.3
MS.gene057806.t1	AT2G24400	49.180	61	30	1	21	81	64	123	5.49e-12	58.5
MS.gene057806.t1	AT4G00880	43.590	78	38	3	3	78	13	86	7.80e-12	57.0
MS.gene057806.t1	AT2G45210	45.902	61	29	2	16	73	74	133	1.24e-11	57.4
MS.gene057806.t1	AT3G60690	42.647	68	35	2	21	85	88	154	1.60e-11	57.0
MS.gene057806.t1	AT4G12410	47.541	61	28	2	21	78	76	135	2.82e-11	56.2

Find 22 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGCTTGGTTCAACAGTTCT+TGG	0.219724	4.4:+36713848	None:intergenic
GTTTGGTTCAAATATGATAT+AGG	0.322369	4.4:+36713877	None:intergenic
GGCATCATCTTCAAAAGGTT+TGG	0.355226	4.4:-36713959	MS.gene057806:CDS
TTTGGTTCAAATATGATATA+GGG	0.362947	4.4:+36713878	None:intergenic
TACACTGCTAGATACCCTTT+TGG	0.399276	4.4:+36713931	None:intergenic
ACAGTTCTTGGAATGAGGTT+TGG	0.410916	4.4:+36713860	None:intergenic
ATCTGATGATACTTGGTAAA+CGG	0.429253	4.4:+36713986	None:intergenic
ATTCAAATTGTTCTTCAGCT+TGG	0.462367	4.4:+36713833	None:intergenic
AAAGGTTTGGACGTGCCAAA+AGG	0.464288	4.4:-36713946	MS.gene057806:CDS
GTTCAACAGTTCTTGGAATG+AGG	0.477913	4.4:+36713855	None:intergenic
TCAAGGGCATCATCTTCAAA+AGG	0.490872	4.4:-36713964	MS.gene057806:CDS
TACCAAGTATCATCAGATCA+AGG	0.490956	4.4:-36713981	MS.gene057806:CDS
GCCCTTGATCTGATGATACT+TGG	0.512708	4.4:+36713979	None:intergenic
AAGGTTTGGACGTGCCAAAA+GGG	0.538442	4.4:-36713945	MS.gene057806:CDS
TTTGAATATGACCATCCAAT+GGG	0.542663	4.4:-36713817	MS.gene057806:CDS
ATTTGAATATGACCATCCAA+TGG	0.559485	4.4:-36713818	MS.gene057806:CDS
AAGAAAATCTCTTCTCTGCA+AGG	0.560279	4.4:+36713781	None:intergenic
TAGTTAGACCACCCATTGGA+TGG	0.598853	4.4:+36713806	None:intergenic
ACCAAGTATCATCAGATCAA+GGG	0.600732	4.4:-36713980	MS.gene057806:CDS
GGAATAGTTAGACCACCCAT+TGG	0.610953	4.4:+36713802	None:intergenic
GAATATGACCATCCAATGGG+TGG	0.646056	4.4:-36713814	MS.gene057806:CDS
GGGTATCTAGCAGTGTACGT+AGG	0.686244	4.4:-36713925	MS.gene057806:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGGTTCAAATATGATATA+GGG	+	chr4.4:36713875-36713894	None:intergenic	20.0%
!!	GTTTGGTTCAAATATGATAT+AGG	+	chr4.4:36713876-36713895	None:intergenic	25.0%
	ATCTGATGATACTTGGTAAA+CGG	+	chr4.4:36713767-36713786	None:intergenic	30.0%
	ATTCAAATTGTTCTTCAGCT+TGG	+	chr4.4:36713920-36713939	None:intergenic	30.0%
	ATTTGAATATGACCATCCAA+TGG	-	chr4.4:36713932-36713951	MS.gene057806:CDS	30.0%
	TTTGAATATGACCATCCAAT+GGG	-	chr4.4:36713933-36713952	MS.gene057806:CDS	30.0%
	AAGAAAATCTCTTCTCTGCA+AGG	+	chr4.4:36713972-36713991	None:intergenic	35.0%
	ACCAAGTATCATCAGATCAA+GGG	-	chr4.4:36713770-36713789	MS.gene057806:CDS	35.0%
	ACGTAGGAGAAAAAATGAAG+CGG	-	chr4.4:36713841-36713860	MS.gene057806:CDS	35.0%
	TACCAAGTATCATCAGATCA+AGG	-	chr4.4:36713769-36713788	MS.gene057806:CDS	35.0%
	ACAGTTCTTGGAATGAGGTT+TGG	+	chr4.4:36713893-36713912	None:intergenic	40.0%
	GGCATCATCTTCAAAAGGTT+TGG	-	chr4.4:36713791-36713810	MS.gene057806:CDS	40.0%
	GTTCAACAGTTCTTGGAATG+AGG	+	chr4.4:36713898-36713917	None:intergenic	40.0%
!	TACACTGCTAGATACCCTTT+TGG	+	chr4.4:36713822-36713841	None:intergenic	40.0%
!!	TCAAGGGCATCATCTTCAAA+AGG	-	chr4.4:36713786-36713805	MS.gene057806:CDS	40.0%
	AAAGGTTTGGACGTGCCAAA+AGG	-	chr4.4:36713804-36713823	MS.gene057806:CDS	45.0%
	AAGGTTTGGACGTGCCAAAA+GGG	-	chr4.4:36713805-36713824	MS.gene057806:CDS	45.0%
	CAGCTTGGTTCAACAGTTCT+TGG	+	chr4.4:36713905-36713924	None:intergenic	45.0%
	GAATATGACCATCCAATGGG+TGG	-	chr4.4:36713936-36713955	MS.gene057806:CDS	45.0%
	GCCCTTGATCTGATGATACT+TGG	+	chr4.4:36713774-36713793	None:intergenic	45.0%
	GGAATAGTTAGACCACCCAT+TGG	+	chr4.4:36713951-36713970	None:intergenic	45.0%
	TAGTTAGACCACCCATTGGA+TGG	+	chr4.4:36713947-36713966	None:intergenic	45.0%
	GGGTATCTAGCAGTGTACGT+AGG	-	chr4.4:36713825-36713844	MS.gene057806:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	36713756	36714016	36713756	ID=MS.gene057806
chr4.4	mRNA	36713756	36714016	36713756	ID=MS.gene057806.t1;Parent=MS.gene057806
chr4.4	exon	36713756	36714016	36713756	ID=MS.gene057806.t1.exon1;Parent=MS.gene057806.t1
chr4.4	CDS	36713756	36714016	36713756	ID=cds.MS.gene057806.t1;Parent=MS.gene057806.t1

Gene Sequence	>MS.gene057806 ATGGGTTTCCGTTTACCAAGTATCATCAGATCAAGGGCATCATCTTCAAAAGGTTTGGACGTGCCAAAAGGGTATCTAGCAGTGTACGTAGGAGAAAAAATGAAGCGGTTTGTGATCCCTATATCATATTTGAACCAAACCTCATTCCAAGAACTGTTGAACCAAGCTGAAGAACAATTTGAATATGACCATCCAATGGGTGGTCTAACTATTCCTTGCAGAGAAGAGATTTTCTTAGATATCACTTCTCACTTGAATTGA
Protein sequence	>MS.gene057806.t1 MGFRLPSIIRSRASSSKGLDVPKGYLAVYVGEKMKRFVIPISYLNQTSFQELLNQAEEQFEYDHPMGGLTIPCREEIFLDITSHLN