AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066264


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066264.t1 MTR_1g099550 85.843 332 16 5 1 320 1 313 0.0 550
MS.gene066264.t1 MTR_8g099045 29.805 359 160 12 1 290 1 336 1.61e-25 105
MS.gene066264.t1 MTR_5g009870 38.767 227 89 12 104 295 159 370 6.02e-25 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066264.t1 AT5G52870 33.117 308 150 12 1 294 1 266 1.75e-28 112
MS.gene066264.t1 AT5G52870 33.117 308 150 12 1 294 1 266 1.92e-28 111
MS.gene066264.t1 AT1G64080 44.444 126 40 3 199 295 266 390 8.70e-18 84.0

Find 57 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGAAACTCGCTCTGTTT+TGG 0.159958 1.1:-71722839 None:intergenic
CTTTAAACTTCCTCAAGTTC+TGG 0.310344 1.1:+71722278 MS.gene066264:CDS
CACCGGCGGTTGGCTCTTTG+AGG 0.337288 1.1:+71722983 MS.gene066264:CDS
GAAACTTTGGTGGCAAAATT+CGG 0.355433 1.1:+71722797 MS.gene066264:CDS
GATTAAGCCTCTTTATGTTA+AGG 0.375290 1.1:+71722888 MS.gene066264:CDS
TTTGTTGTCGAAATCATTCA+TGG 0.384507 1.1:-71722401 None:intergenic
TTCGAAACCTTAACATAAAG+AGG 0.384723 1.1:-71722895 None:intergenic
GAAAGTGGTGACGAAGCATT+TGG 0.391796 1.1:+71723005 MS.gene066264:CDS
TTGGACATGTGGGTCGTCAT+TGG 0.416385 1.1:-71722425 None:intergenic
ATGGATGTTGTTGGTGGTGA+TGG 0.423562 1.1:+71723299 MS.gene066264:CDS
GAGCTTGTTCTCCGGCGAAC+AGG 0.426734 1.1:+71723058 MS.gene066264:CDS
TTCAGAAAACAGAGAAGAAC+TGG 0.432309 1.1:+71722637 MS.gene066264:CDS
GATTCTTCAATGGATGTTGT+TGG 0.446904 1.1:+71723290 MS.gene066264:intron
AGGTCGTCGGGTACGGTGGC+CGG 0.454518 1.1:+71723036 MS.gene066264:CDS
TGCTGATCTTAGAAGTGAAA+TGG 0.459585 1.1:-71722593 None:intergenic
TTCGAAGAGGTATGGTGATA+AGG 0.470661 1.1:+71722912 MS.gene066264:CDS
TAAGAGTAGGTCGTCGGGTA+CGG 0.475412 1.1:+71723029 MS.gene066264:CDS
GGTGGCAAAATTCGGAATCA+AGG 0.479447 1.1:+71722805 MS.gene066264:CDS
CCGTCTTCTTCACCGGCGGT+TGG 0.485938 1.1:+71722973 MS.gene066264:CDS
GTTAAGGTTTCGAAGAGGTA+TGG 0.486790 1.1:+71722904 MS.gene066264:CDS
GGTGATAAGGTGAAATTCTC+CGG 0.500103 1.1:+71722925 MS.gene066264:CDS
TGCTTCGCCGTCTTCTTCAC+CGG 0.518483 1.1:+71722966 MS.gene066264:CDS
CTGTTCTCTCTACTAAGCAT+TGG 0.523196 1.1:-71722769 None:intergenic
GAGAACAGCACAAGAAACTT+TGG 0.523674 1.1:+71722784 MS.gene066264:CDS
AAAGTGGTGACGAAGCATTT+GGG 0.526267 1.1:+71723006 MS.gene066264:CDS
AAGATGAGGAACAGTGATGT+TGG 0.527025 1.1:-71722320 None:intergenic
GAGGAAGTTTAAAGCATCCA+TGG 0.529668 1.1:-71722269 None:intergenic
AGCTACCGTCATCCCCTCGC+CGG 0.534216 1.1:-71722944 None:intergenic
CAAAACAGAGCGAGTTTCAA+AGG 0.536455 1.1:+71722840 MS.gene066264:CDS
TGTTGTTCTTCCAGAACTTG+AGG 0.537675 1.1:-71722288 None:intergenic
GGTTGGCTCTTTGAGGAAAG+TGG 0.542571 1.1:+71722990 MS.gene066264:CDS
TTGGGTAAGAGTAGGTCGTC+GGG 0.544095 1.1:+71723024 MS.gene066264:CDS
AAGGTGAAATTCTCCGGCGA+GGG 0.550532 1.1:+71722931 MS.gene066264:CDS
AAGGTAAAGATTCTGAAACT+TGG 0.555444 1.1:-71722616 None:intergenic
AGAAGTGAAATGGGTGATTG+TGG 0.566819 1.1:-71722583 None:intergenic
GGTGGCCGGAGCTTGTTCTC+CGG 0.566890 1.1:+71723050 MS.gene066264:CDS
ATTCTCCGGCGAGGGGATGA+CGG 0.568736 1.1:+71722939 MS.gene066264:CDS
GCTGATCTTAGAAGTGAAAT+GGG 0.571175 1.1:-71722592 None:intergenic
TTCCTCAAAGAGCCAACCGC+CGG 0.578853 1.1:-71722985 None:intergenic
CGAAGCATTTGGGTAAGAGT+AGG 0.585768 1.1:+71723016 MS.gene066264:CDS
TTTGGGTAAGAGTAGGTCGT+CGG 0.592489 1.1:+71723023 MS.gene066264:CDS
ACGCTGTTGCAGCAACATGA+TGG 0.598349 1.1:+71723090 MS.gene066264:CDS
GTATCATCACTCCTGTTCGC+CGG 0.598908 1.1:-71723069 None:intergenic
TCTTCAATGGATGTTGTTGG+TGG 0.599000 1.1:+71723293 MS.gene066264:CDS
CCAACCGCCGGTGAAGAAGA+CGG 0.604637 1.1:-71722973 None:intergenic
GTTCGCCGGAGAACAAGCTC+CGG 0.605965 1.1:-71723055 None:intergenic
GAGTAGGTCGTCGGGTACGG+TGG 0.623063 1.1:+71723032 MS.gene066264:CDS
AACAGCACAAGAAACTTTGG+TGG 0.628576 1.1:+71722787 MS.gene066264:CDS
TTCGCCGTCTTCTTCACCGG+CGG 0.640158 1.1:+71722969 MS.gene066264:CDS
TCTGTTTCTACAACAAGATG+AGG 0.645146 1.1:-71722334 None:intergenic
AAGAGATCCTTCAATTCCAG+AGG 0.646564 1.1:+71723138 MS.gene066264:CDS
AGGTGAAATTCTCCGGCGAG+GGG 0.653189 1.1:+71722932 MS.gene066264:CDS
GAATCAGAAGAAAGAAACCA+AGG 0.653413 1.1:+71722702 MS.gene066264:CDS
TAAGGTGAAATTCTCCGGCG+AGG 0.664840 1.1:+71722930 MS.gene066264:CDS
ATGAGGAACAGTGATGTTGG+AGG 0.666650 1.1:-71722317 None:intergenic
TTTATGTTAAGGTTTCGAAG+AGG 0.679040 1.1:+71722899 MS.gene066264:CDS
AATTCGGAATCAAGGAACAG+AGG 0.681760 1.1:+71722813 MS.gene066264:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATATGATTATTTTTGAA+AGG + chr1.1:71723210-71723229 MS.gene066264:intron 10.0%
!!! ATAATTTTCAGATTCTTCAA+TGG + chr1.1:71723280-71723299 MS.gene066264:intron 20.0%
!! TTCAACAAGAAATTCTTTTG+AGG + chr1.1:71723331-71723350 MS.gene066264:CDS 25.0%
!!! ATAAGAAGTGTTGTTTTTTG+AGG - chr1.1:71722505-71722524 None:intergenic 25.0%
GATTAAGCCTCTTTATGTTA+AGG + chr1.1:71722888-71722907 MS.gene066264:CDS 30.0%
TAGTTTCACAGAAAAAACCT+TGG - chr1.1:71722722-71722741 None:intergenic 30.0%
TTCGAAACCTTAACATAAAG+AGG - chr1.1:71722898-71722917 None:intergenic 30.0%
TTTATGTTAAGGTTTCGAAG+AGG + chr1.1:71722899-71722918 MS.gene066264:CDS 30.0%
! GTTTTCTGTTTTGTGAGTTT+TGG - chr1.1:71722452-71722471 None:intergenic 30.0%
! TGTGAAACAAATTTTACCTC+TGG - chr1.1:71723157-71723176 None:intergenic 30.0%
! TTTGTTGTCGAAATCATTCA+TGG - chr1.1:71722404-71722423 None:intergenic 30.0%
!! AAGGTAAAGATTCTGAAACT+TGG - chr1.1:71722619-71722638 None:intergenic 30.0%
!! ATTAGTTTCAGTTTTCTCGT+AGG - chr1.1:71722479-71722498 None:intergenic 30.0%
!!! AATTTTACCTCTGGAATTGA+AGG - chr1.1:71723148-71723167 None:intergenic 30.0%
!!! AGCACTTTTCTTTTTGAGAT+AGG - chr1.1:71722541-71722560 None:intergenic 30.0%
CTTTAAACTTCCTCAAGTTC+TGG + chr1.1:71722278-71722297 MS.gene066264:CDS 35.0%
GAATCAGAAGAAAGAAACCA+AGG + chr1.1:71722702-71722721 MS.gene066264:CDS 35.0%
GATTCTTCAATGGATGTTGT+TGG + chr1.1:71723290-71723309 MS.gene066264:intron 35.0%
GCTGATCTTAGAAGTGAAAT+GGG - chr1.1:71722595-71722614 None:intergenic 35.0%
TGCTGATCTTAGAAGTGAAA+TGG - chr1.1:71722596-71722615 None:intergenic 35.0%
TTCAGAAAACAGAGAAGAAC+TGG + chr1.1:71722637-71722656 MS.gene066264:CDS 35.0%
! AGTTCTTCTCTGTTTTCTGA+AGG - chr1.1:71722638-71722657 None:intergenic 35.0%
! GAAACTTTGGTGGCAAAATT+CGG + chr1.1:71722797-71722816 MS.gene066264:CDS 35.0%
!! TCTGTTTCTACAACAAGATG+AGG - chr1.1:71722337-71722356 None:intergenic 35.0%
!! TTTGAAACTCGCTCTGTTTT+GGG - chr1.1:71722841-71722860 None:intergenic 35.0%
!!! CTGTTTTGTGAGTTTTGGTT+TGG - chr1.1:71722447-71722466 None:intergenic 35.0%
!!! CTTTTTGAGATAGGTTCGTT+TGG - chr1.1:71722532-71722551 None:intergenic 35.0%
!!! GGTGATTGTGGTTTTGAAAT+GGG - chr1.1:71722574-71722593 None:intergenic 35.0%
!!! GGTTTTGAAATGGGTTCAAT+TGG - chr1.1:71722565-71722584 None:intergenic 35.0%
AACAGCACAAGAAACTTTGG+TGG + chr1.1:71722787-71722806 MS.gene066264:CDS 40.0%
AAGAGATCCTTCAATTCCAG+AGG + chr1.1:71723138-71723157 MS.gene066264:CDS 40.0%
AAGATGAGGAACAGTGATGT+TGG - chr1.1:71722323-71722342 None:intergenic 40.0%
AATTCGGAATCAAGGAACAG+AGG + chr1.1:71722813-71722832 MS.gene066264:CDS 40.0%
AGAAGTGAAATGGGTGATTG+TGG - chr1.1:71722586-71722605 None:intergenic 40.0%
CAAAACAGAGCGAGTTTCAA+AGG + chr1.1:71722840-71722859 MS.gene066264:CDS 40.0%
CTGTTCTCTCTACTAAGCAT+TGG - chr1.1:71722772-71722791 None:intergenic 40.0%
GAGAACAGCACAAGAAACTT+TGG + chr1.1:71722784-71722803 MS.gene066264:CDS 40.0%
GGTGATAAGGTGAAATTCTC+CGG + chr1.1:71722925-71722944 MS.gene066264:CDS 40.0%
GTTAAGGTTTCGAAGAGGTA+TGG + chr1.1:71722904-71722923 MS.gene066264:CDS 40.0%
TCTTCAATGGATGTTGTTGG+TGG + chr1.1:71723293-71723312 MS.gene066264:CDS 40.0%
TGTTGTTCTTCCAGAACTTG+AGG - chr1.1:71722291-71722310 None:intergenic 40.0%
! AAAGTGGTGACGAAGCATTT+GGG + chr1.1:71723006-71723025 MS.gene066264:CDS 40.0%
! TTCGAAGAGGTATGGTGATA+AGG + chr1.1:71722912-71722931 MS.gene066264:CDS 40.0%
!!! CTTTGAAACTCGCTCTGTTT+TGG - chr1.1:71722842-71722861 None:intergenic 40.0%
!!! GAGTTTTGGTTTGGACATGT+GGG - chr1.1:71722438-71722457 None:intergenic 40.0%
!!! GGGTGATTGTGGTTTTGAAA+TGG - chr1.1:71722575-71722594 None:intergenic 40.0%
!!! TGAGTTTTGGTTTGGACATG+TGG - chr1.1:71722439-71722458 None:intergenic 40.0%
ATGAGGAACAGTGATGTTGG+AGG - chr1.1:71722320-71722339 None:intergenic 45.0%
GGTGGCAAAATTCGGAATCA+AGG + chr1.1:71722805-71722824 MS.gene066264:CDS 45.0%
TTTGGGTAAGAGTAGGTCGT+CGG + chr1.1:71723023-71723042 MS.gene066264:CDS 45.0%
! CGAAGCATTTGGGTAAGAGT+AGG + chr1.1:71723016-71723035 MS.gene066264:CDS 45.0%
! GAAAGTGGTGACGAAGCATT+TGG + chr1.1:71723005-71723024 MS.gene066264:CDS 45.0%
!! ATGGATGTTGTTGGTGGTGA+TGG + chr1.1:71723299-71723318 MS.gene066264:CDS 45.0%
AAGGTGAAATTCTCCGGCGA+GGG + chr1.1:71722931-71722950 MS.gene066264:CDS 50.0%
ACGCTGTTGCAGCAACATGA+TGG + chr1.1:71723090-71723109 MS.gene066264:CDS 50.0%
GTATCATCACTCCTGTTCGC+CGG - chr1.1:71723072-71723091 None:intergenic 50.0%
TAAGAGTAGGTCGTCGGGTA+CGG + chr1.1:71723029-71723048 MS.gene066264:CDS 50.0%
TAAGGTGAAATTCTCCGGCG+AGG + chr1.1:71722930-71722949 MS.gene066264:CDS 50.0%
TTGGACATGTGGGTCGTCAT+TGG - chr1.1:71722428-71722447 None:intergenic 50.0%
TTGGGTAAGAGTAGGTCGTC+GGG + chr1.1:71723024-71723043 MS.gene066264:CDS 50.0%
! GGTTGGCTCTTTGAGGAAAG+TGG + chr1.1:71722990-71723009 MS.gene066264:CDS 50.0%
AGGTGAAATTCTCCGGCGAG+GGG + chr1.1:71722932-71722951 MS.gene066264:CDS 55.0%
TGCTTCGCCGTCTTCTTCAC+CGG + chr1.1:71722966-71722985 MS.gene066264:CDS 55.0%
TTCCTCAAAGAGCCAACCGC+CGG - chr1.1:71722988-71723007 None:intergenic 55.0%
ATTCTCCGGCGAGGGGATGA+CGG + chr1.1:71722939-71722958 MS.gene066264:CDS 60.0%
CCAACCGCCGGTGAAGAAGA+CGG - chr1.1:71722976-71722995 None:intergenic 60.0%
GTTCGCCGGAGAACAAGCTC+CGG - chr1.1:71723058-71723077 None:intergenic 60.0%
TTCGCCGTCTTCTTCACCGG+CGG + chr1.1:71722969-71722988 MS.gene066264:CDS 60.0%
! GAGCTTGTTCTCCGGCGAAC+AGG + chr1.1:71723058-71723077 MS.gene066264:CDS 60.0%
AGCTACCGTCATCCCCTCGC+CGG - chr1.1:71722947-71722966 None:intergenic 65.0%
CCGTCTTCTTCACCGGCGGT+TGG + chr1.1:71722973-71722992 MS.gene066264:CDS 65.0%
GAGTAGGTCGTCGGGTACGG+TGG + chr1.1:71723032-71723051 MS.gene066264:CDS 65.0%
! CACCGGCGGTTGGCTCTTTG+AGG + chr1.1:71722983-71723002 MS.gene066264:CDS 65.0%
! GGTGGCCGGAGCTTGTTCTC+CGG + chr1.1:71723050-71723069 MS.gene066264:CDS 65.0%
AGGTCGTCGGGTACGGTGGC+CGG + chr1.1:71723036-71723055 MS.gene066264:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 71722271 71723364 71722271 ID=MS.gene066264
chr1.1 mRNA 71722271 71723364 71722271 ID=MS.gene066264.t1;Parent=MS.gene066264
chr1.1 exon 71722271 71723159 71722271 ID=MS.gene066264.t1.exon1;Parent=MS.gene066264.t1
chr1.1 CDS 71722271 71723159 71722271 ID=cds.MS.gene066264.t1;Parent=MS.gene066264.t1
chr1.1 exon 71723291 71723364 71723291 ID=MS.gene066264.t1.exon2;Parent=MS.gene066264.t1
chr1.1 CDS 71723291 71723364 71723291 ID=cds.MS.gene066264.t1;Parent=MS.gene066264.t1
Gene Sequence

>MS.gene066264

ATGGATGCTTTAAACTTCCTCAAGTTCTGGAAGAACAACACAACAACCTCCAACATCACTGTTCCTCATCTTGTTGTAGAAACAGACAGCGAATCAGACGAAGATGACTCATTCTTCGACTTAGAACTCTCCATGAATGATTTCGACAACAAAACCAATGACGACCCACATGTCCAAACCAAAACTCACAAAACAGAAAACATTACCTACGAGAAAACTGAAACTAATGTTCCTCAAAAAACAACACTTCTTATGTCTCCAAACGAACCTATCTCAAAAAGAAAAGTGCTTCCAATTGAACCCATTTCAAAACCACAATCACCCATTTCACTTCTAAGATCAGCACCAAGTTTCAGAATCTTTACCTTCAGAAAACAGAGAAGAACTGGTTCTGAAAAAACAGACGAGTTTAGAAAAACAGAGTGTGAAAAGAATCAGAAGAAAGAAACCAAGGTTTTTTCTGTGAAACTAAATATAGAAGAAGATTTTCATTCTACTCCAATGCTTAGTAGAGAGAACAGCACAAGAAACTTTGGTGGCAAAATTCGGAATCAAGGAACAGAGGAACCCAAAACAGAGCGAGTTTCAAAGGAGATTTTGCAGAAATATTTGAAACTGATTAAGCCTCTTTATGTTAAGGTTTCGAAGAGGTATGGTGATAAGGTGAAATTCTCCGGCGAGGGGATGACGGTAGCTGCTTCGCCGTCTTCTTCACCGGCGGTTGGCTCTTTGAGGAAAGTGGTGACGAAGCATTTGGGTAAGAGTAGGTCGTCGGGTACGGTGGCCGGAGCTTGTTCTCCGGCGAACAGGAGTGATGATACGCTGTTGCAGCAACATGATGGAATTCAAAGTGCTATTCTTCATTGCAAGAGATCCTTCAATTCCAGAGGTAAAATTTGTTTCACAAATAATATTTTTTTATTCATATATTAATTATGCTAAATATGATTATTTTTGAAAGGATATGCTAAATATGATTTGCAATGTCAATTAATTATAGTTAATTTTTATAATTTTCAGATTCTTCAATGGATGTTGTTGGTGGTGATGGATCAATGTATTCAACAAGAAATTCTTTTGAGGATGAAGTGTGA

Protein sequence

>MS.gene066264.t1

MDALNFLKFWKNNTTTSNITVPHLVVETDSESDEDDSFFDLELSMNDFDNKTNDDPHVQTKTHKTENITYEKTETNVPQKTTLLMSPNEPISKRKVLPIEPISKPQSPISLLRSAPSFRIFTFRKQRRTGSEKTDEFRKTECEKNQKKETKVFSVKLNIEEDFHSTPMLSRENSTRNFGGKIRNQGTEEPKTERVSKEILQKYLKLIKPLYVKVSKRYGDKVKFSGEGMTVAASPSSSPAVGSLRKVVTKHLGKSRSSGTVAGACSPANRSDDTLLQQHDGIQSAILHCKRSFNSRDSSMDVVGGDGSMYSTRNSFEDEV