AlfalfaGEDB | MS.gene068529

M. sativa cultivar XinJiangDaYe / MS.gene068529

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068529.t1	XP_003590511.1	100	136	0	0	1	136	1	136	1.00E-44	189.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068529.t1	O22582	100.0	97	0	0	40	136	51	147	3.6e-46	185.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068529.t1	G7IBM3	100.0	136	0	0	1	136	1	136	7.4e-45	189.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene068529	0.818917	1.53E-52	-1.69E-46
MS.gene049159	MS.gene068529	0.840704	7.41E-58	-1.69E-46
MS.gene049254	MS.gene068529	0.832009	1.21E-55	-1.69E-46
MS.gene049343	MS.gene068529	0.829749	4.32E-55	-1.69E-46
MS.gene049435	MS.gene068529	0.807456	5.01E-50	-1.69E-46
MS.gene049452	MS.gene068529	0.819124	1.37E-52	-1.69E-46
MS.gene049821	MS.gene068529	0.822636	2.13E-53	-1.69E-46
MS.gene049850	MS.gene068529	0.810312	1.23E-50	-1.69E-46
MS.gene049983	MS.gene068529	0.830056	3.64E-55	-1.69E-46
MS.gene050605	MS.gene068529	0.809419	1.91E-50	-1.69E-46
MS.gene050901	MS.gene068529	0.808718	2.70E-50	-1.69E-46
MS.gene050905	MS.gene068529	-0.805796	1.12E-49	-1.69E-46
MS.gene051066	MS.gene068529	0.80096	1.13E-48	-1.69E-46
MS.gene051198	MS.gene068529	0.830151	3.45E-55	-1.69E-46
MS.gene051328	MS.gene068529	0.80229	6.02E-49	-1.69E-46
MS.gene051597	MS.gene068529	0.805702	1.17E-49	-1.69E-46
MS.gene051744	MS.gene068529	0.822993	1.76E-53	-1.69E-46
MS.gene05188	MS.gene068529	0.821606	3.69E-53	-1.69E-46
MS.gene051975	MS.gene068529	0.811841	5.72E-51	-1.69E-46
MS.gene052012	MS.gene068529	0.812238	4.69E-51	-1.69E-46
MS.gene052516	MS.gene068529	0.801063	1.07E-48	-1.69E-46
MS.gene052519	MS.gene068529	0.816868	4.43E-52	-1.69E-46
MS.gene052582	MS.gene068529	0.824106	9.66E-54	-1.69E-46
MS.gene052584	MS.gene068529	0.827663	1.38E-54	-1.69E-46
MS.gene052773	MS.gene068529	0.829449	5.12E-55	-1.69E-46
MS.gene052863	MS.gene068529	0.810974	8.82E-51	-1.69E-46
MS.gene053008	MS.gene068529	0.835587	1.54E-56	-1.69E-46
MS.gene05308	MS.gene068529	0.816687	4.86E-52	-1.69E-46
MS.gene053226	MS.gene068529	0.843433	1.41E-58	-1.69E-46
MS.gene053542	MS.gene068529	0.802802	4.72E-49	-1.69E-46
MS.gene05364	MS.gene068529	0.813306	2.74E-51	-1.69E-46
MS.gene053931	MS.gene068529	0.812854	3.44E-51	-1.69E-46
MS.gene05415	MS.gene068529	0.81471	1.34E-51	-1.69E-46
MS.gene054236	MS.gene068529	0.803125	4.05E-49	-1.69E-46
MS.gene054651	MS.gene068529	0.835837	1.33E-56	-1.69E-46
MS.gene054657	MS.gene068529	0.836964	6.87E-57	-1.69E-46
MS.gene054658	MS.gene068529	0.81997	8.78E-53	-1.69E-46
MS.gene054775	MS.gene068529	0.814864	1.24E-51	-1.69E-46
MS.gene05517	MS.gene068529	0.834971	2.20E-56	-1.69E-46
MS.gene055222	MS.gene068529	0.83003	3.69E-55	-1.69E-46
MS.gene055512	MS.gene068529	0.804545	2.05E-49	-1.69E-46
MS.gene055513	MS.gene068529	0.803045	4.20E-49	-1.69E-46
MS.gene055623	MS.gene068529	0.813679	2.27E-51	-1.69E-46
MS.gene055694	MS.gene068529	0.826776	2.25E-54	-1.69E-46
MS.gene055818	MS.gene068529	0.804522	2.07E-49	-1.69E-46
MS.gene055841	MS.gene068529	0.810512	1.11E-50	-1.69E-46
MS.gene055926	MS.gene068529	0.804387	2.21E-49	-1.69E-46
MS.gene056050	MS.gene068529	0.826565	2.53E-54	-1.69E-46
MS.gene056173	MS.gene068529	0.802387	5.75E-49	-1.69E-46
MS.gene056174	MS.gene068529	0.814063	1.86E-51	-1.69E-46
MS.gene056215	MS.gene068529	0.83302	6.78E-56	-1.69E-46
MS.gene056308	MS.gene068529	0.805373	1.38E-49	-1.69E-46
MS.gene056366	MS.gene068529	0.813061	3.10E-51	-1.69E-46
MS.gene056506	MS.gene068529	0.800015	1.76E-48	-1.69E-46
MS.gene056595	MS.gene068529	0.822625	2.14E-53	-1.69E-46
MS.gene056776	MS.gene068529	0.82218	2.72E-53	-1.69E-46
MS.gene05690	MS.gene068529	0.805468	1.32E-49	-1.69E-46
MS.gene057034	MS.gene068529	0.816825	4.53E-52	-1.69E-46
MS.gene057208	MS.gene068529	0.852567	4.24E-61	-1.69E-46
MS.gene057444	MS.gene068529	0.838364	3.00E-57	-1.69E-46
MS.gene057598	MS.gene068529	0.830083	3.59E-55	-1.69E-46
MS.gene057693	MS.gene068529	0.822576	2.20E-53	-1.69E-46
MS.gene057855	MS.gene068529	0.852975	3.24E-61	-1.69E-46
MS.gene057915	MS.gene068529	0.807552	4.78E-50	-1.69E-46
MS.gene058009	MS.gene068529	0.815166	1.06E-51	-1.69E-46
MS.gene058010	MS.gene068529	0.819394	1.19E-52	-1.69E-46
MS.gene058146	MS.gene068529	0.814746	1.32E-51	-1.69E-46
MS.gene05847	MS.gene068529	0.817568	3.08E-52	-1.69E-46
MS.gene058548	MS.gene068529	0.812495	4.12E-51	-1.69E-46
MS.gene058549	MS.gene068529	0.80026	1.57E-48	-1.69E-46
MS.gene058595	MS.gene068529	0.818073	2.37E-52	-1.69E-46
MS.gene058625	MS.gene068529	0.809464	1.87E-50	-1.69E-46
MS.gene058781	MS.gene068529	0.817136	3.86E-52	-1.69E-46
MS.gene058783	MS.gene068529	0.829578	4.76E-55	-1.69E-46
MS.gene058976	MS.gene068529	0.86747	1.34E-65	-1.69E-46
MS.gene058979	MS.gene068529	0.86615	3.52E-65	-1.69E-46
MS.gene059625	MS.gene068529	0.812561	3.98E-51	-1.69E-46
MS.gene05979	MS.gene068529	0.82546	4.63E-54	-1.69E-46
MS.gene059842	MS.gene068529	0.838679	2.49E-57	-1.69E-46
MS.gene060310	MS.gene068529	0.835125	2.01E-56	-1.69E-46
MS.gene060365	MS.gene068529	0.810414	1.17E-50	-1.69E-46
MS.gene060429	MS.gene068529	0.847338	1.23E-59	-1.69E-46
MS.gene060572	MS.gene068529	0.80261	5.17E-49	-1.69E-46
MS.gene060803	MS.gene068529	0.825657	4.16E-54	-1.69E-46
MS.gene060935	MS.gene068529	0.82516	5.45E-54	-1.69E-46
MS.gene060937	MS.gene068529	0.811259	7.66E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene068529	MS.gene81051	PPI
MS.gene066348	MS.gene068529	PPI
MS.gene035396	MS.gene068529	PPI
MS.gene049824	MS.gene068529	PPI
MS.gene75219	MS.gene068529	PPI
MS.gene068529	MS.gene066301	PPI
MS.gene025426	MS.gene068529	PPI
MS.gene068529	MS.gene051008	PPI
MS.gene004127	MS.gene068529	PPI
MS.gene025035	MS.gene068529	PPI
MS.gene068529	MS.gene84498	PPI
MS.gene068529	MS.gene27326	PPI
MS.gene066300	MS.gene068529	PPI
MS.gene26950	MS.gene068529	PPI
MS.gene041623	MS.gene068529	PPI
MS.gene004126	MS.gene068529	PPI
MS.gene025425	MS.gene068529	PPI
MS.gene068529	MS.gene65249	PPI
MS.gene068529	MS.gene08465	PPI
MS.gene049822	MS.gene068529	PPI
MS.gene63143	MS.gene068529	PPI
MS.gene068529	MS.gene99525	PPI
MS.gene65007	MS.gene068529	PPI
MS.gene24295	MS.gene068529	PPI
MS.gene63953	MS.gene068529	PPI
MS.gene068529	MS.gene64653	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068529.t1	MTR_1g068600	99.265	136	1	0	1	136	1	136	1.04e-93	266
MS.gene068529.t1	MTR_4g063240	85.034	147	11	2	1	136	1	147	1.77e-81	236
MS.gene068529.t1	MTR_4g063220	85.811	148	9	2	1	136	1	148	4.40e-78	228
MS.gene068529.t1	MTR_4g063990	85.811	148	9	2	1	136	1	148	4.40e-78	228
MS.gene068529.t1	MTR_4g063200	85.811	148	9	2	1	136	1	148	4.40e-78	228
MS.gene068529.t1	MTR_4g064020	85.811	148	9	2	1	136	1	148	4.40e-78	228
MS.gene068529.t1	MTR_1g090780	91.304	138	9	2	1	136	1	137	5.11e-78	227
MS.gene068529.t1	MTR_1g492910	91.304	138	9	2	1	136	1	137	5.89e-78	227
MS.gene068529.t1	MTR_4g071180	85.811	148	9	2	1	136	1	148	4.30e-77	225
MS.gene068529.t1	MTR_4g070240	85.811	148	9	2	1	136	1	148	4.30e-77	225
MS.gene068529.t1	MTR_4g088025	86.713	143	12	2	1	136	1	143	9.26e-76	221
MS.gene068529.t1	MTR_4g070245	84.459	148	11	2	1	136	1	148	1.54e-75	221
MS.gene068529.t1	MTR_4g063275	83.108	148	13	2	1	136	1	148	4.86e-75	220
MS.gene068529.t1	MTR_4g064983	84.722	144	10	2	1	132	1	144	2.53e-73	219
MS.gene068529.t1	MTR_7g099960	95.370	108	4	1	30	136	31	138	2.89e-64	192
MS.gene068529.t1	MTR_2g084480	62.921	89	33	0	47	135	136	224	9.85e-38	128
MS.gene068529.t1	MTR_8g069990	71.591	88	23	2	8	95	3	88	6.01e-35	117
MS.gene068529.t1	MTR_4g063270	90.196	51	5	0	74	124	1	51	5.77e-27	95.5
MS.gene068529.t1	MTR_7g114580	90.244	41	4	0	96	136	11	51	7.05e-20	77.4
MS.gene068529.t1	MTR_4g032777	39.744	78	47	0	43	120	33	110	1.43e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068529.t1	AT3G45980	83.333	150	11	2	1	136	1	150	6.00e-77	225
MS.gene068529.t1	AT2G37470	88.406	138	13	2	1	135	1	138	7.78e-76	221
MS.gene068529.t1	AT1G07790	83.784	148	12	2	1	136	1	148	2.33e-75	221
MS.gene068529.t1	AT2G28720	83.444	151	10	2	1	136	1	151	2.26e-74	218
MS.gene068529.t1	AT3G53650	87.681	138	15	2	1	136	1	138	1.55e-73	216
MS.gene068529.t1	AT5G59910	87.407	135	14	1	5	136	16	150	6.19e-73	214
MS.gene068529.t1	AT3G46030	88.372	129	12	1	11	136	17	145	1.85e-71	211
MS.gene068529.t1	AT5G02570	83.571	140	11	2	1	136	1	132	1.41e-68	203
MS.gene068529.t1	AT3G09480	80.147	136	17	1	1	136	1	126	1.23e-65	195
MS.gene068529.t1	AT5G22880	81.119	143	17	2	4	136	3	145	2.62e-65	195
MS.gene068529.t1	AT1G08170	62.353	85	32	0	49	133	151	235	8.86e-38	128

Find 30 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGAAAATGTAAATCTTAT+AGG	0.106260	1.1:-50225247	None:intergenic
AATTCAAGAACTCGTAAATT+TGG	0.207431	1.1:-50225496	None:intergenic
TTCTTTCGAGATCTTCTTCT+CGG	0.293193	1.1:-50225181	None:intergenic
GCTGTGCGTTTGGTTCTTCC+TGG	0.321985	1.1:+50225429	MS.gene068529:CDS
TTTGAAGAAATACCTATATC+AGG	0.334896	1.1:-50225288	None:intergenic
GAGATCTTCTTCTCGGCTCT+TGG	0.362586	1.1:-50225174	None:intergenic
GATTCAAACCGCTGTGCGTT+TGG	0.390239	1.1:+50225419	MS.gene068529:CDS
TTTGGTTCTTCCTGGTGAAT+TGG	0.398260	1.1:+50225437	MS.gene068529:CDS
TTTCTCTGCCTTGGGAGCCA+TGG	0.402280	1.1:-50225103	None:intergenic
AAACAGGTTCATCCTGATAT+AGG	0.421612	1.1:+50225276	MS.gene068529:CDS
AGGTATTTCTTCAAAAGCTA+TGG	0.429032	1.1:+50225296	MS.gene068529:CDS
CTTGTTGTAACGAGCCAATC+TGG	0.445538	1.1:-50225373	None:intergenic
TCCGCGGGTTTCTTCTCAGC+GGG	0.464857	1.1:-50225129	None:intergenic
TCTGCTGGTGATTTCTCCGC+GGG	0.470002	1.1:-50225144	None:intergenic
CGCTCAGGAAGCTTCCAGAT+TGG	0.475450	1.1:+50225359	MS.gene068529:CDS
TGCTGTTTCTGAAGGAACTA+AGG	0.493611	1.1:+50225467	MS.gene068529:CDS
GCATGTTTCGCCAATTCACC+AGG	0.516345	1.1:-50225447	None:intergenic
GGTATTTCTTCAAAAGCTAT+GGG	0.517184	1.1:+50225297	MS.gene068529:CDS
GCGAAACATGCTGTTTCTGA+AGG	0.530754	1.1:+50225459	MS.gene068529:CDS
ATTCAGATCCATGGCTCCCA+AGG	0.541897	1.1:+50225095	None:intergenic
GAAACCGACGATTACGTCAC+GGG	0.556515	1.1:+50225395	MS.gene068529:CDS
CTCTGCTGGTGATTTCTCCG+CGG	0.560516	1.1:-50225145	None:intergenic
GAGAAGAAGATCTCGAAAGA+AGG	0.564931	1.1:+50225183	MS.gene068529:CDS
TATCTTTGAGAAACTCGCTC+AGG	0.586744	1.1:+50225344	MS.gene068529:CDS
ATCTCCCGTGACGTAATCGT+CGG	0.590908	1.1:-50225399	None:intergenic
CTCCGCGGGTTTCTTCTCAG+CGG	0.628471	1.1:-50225130	None:intergenic
AAGAAGATCTCGAAAGAAGG+AGG	0.628797	1.1:+50225186	MS.gene068529:CDS
AGAAACCGACGATTACGTCA+CGG	0.672005	1.1:+50225394	MS.gene068529:CDS
ACCCGCTGAGAAGAAACCCG+CGG	0.690098	1.1:+50225128	MS.gene068529:CDS
AGGAAGAACCAAACGCACAG+CGG	0.757762	1.1:-50225427	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTGAAAATGTAAATCTTAT+AGG	-	chr1.1:50225250-50225269	None:intergenic	20.0%
!!!	CTATAAGATTTACATTTTCA+AGG	+	chr1.1:50225248-50225267	MS.gene068529:CDS	20.0%
!	TTTGAAGAAATACCTATATC+AGG	-	chr1.1:50225291-50225310	None:intergenic	25.0%
	AGGTATTTCTTCAAAAGCTA+TGG	+	chr1.1:50225296-50225315	MS.gene068529:CDS	30.0%
	GGTATTTCTTCAAAAGCTAT+GGG	+	chr1.1:50225297-50225316	MS.gene068529:CDS	30.0%
	AAACAGGTTCATCCTGATAT+AGG	+	chr1.1:50225276-50225295	MS.gene068529:CDS	35.0%
	TTCTTTCGAGATCTTCTTCT+CGG	-	chr1.1:50225184-50225203	None:intergenic	35.0%
!!	CATTTTCAAGGTTCTCAAAC+AGG	+	chr1.1:50225260-50225279	MS.gene068529:CDS	35.0%
	AAGAAGATCTCGAAAGAAGG+AGG	+	chr1.1:50225186-50225205	MS.gene068529:CDS	40.0%
	GAGAAGAAGATCTCGAAAGA+AGG	+	chr1.1:50225183-50225202	MS.gene068529:CDS	40.0%
!	TATCTTTGAGAAACTCGCTC+AGG	+	chr1.1:50225344-50225363	MS.gene068529:CDS	40.0%
!!	TGCTGTTTCTGAAGGAACTA+AGG	+	chr1.1:50225467-50225486	MS.gene068529:CDS	40.0%
!!	TTTGGTTCTTCCTGGTGAAT+TGG	+	chr1.1:50225437-50225456	MS.gene068529:CDS	40.0%
	AGAAACCGACGATTACGTCA+CGG	+	chr1.1:50225394-50225413	MS.gene068529:CDS	45.0%
!	CTTGTTGTAACGAGCCAATC+TGG	-	chr1.1:50225376-50225395	None:intergenic	45.0%
!!	GCGAAACATGCTGTTTCTGA+AGG	+	chr1.1:50225459-50225478	MS.gene068529:CDS	45.0%
	AGGAAGAACCAAACGCACAG+CGG	-	chr1.1:50225430-50225449	None:intergenic	50.0%
	ATCTCCCGTGACGTAATCGT+CGG	-	chr1.1:50225402-50225421	None:intergenic	50.0%
	GAAACCGACGATTACGTCAC+GGG	+	chr1.1:50225395-50225414	MS.gene068529:CDS	50.0%
	GAGATCTTCTTCTCGGCTCT+TGG	-	chr1.1:50225177-50225196	None:intergenic	50.0%
	GATTCAAACCGCTGTGCGTT+TGG	+	chr1.1:50225419-50225438	MS.gene068529:CDS	50.0%
	GCATGTTTCGCCAATTCACC+AGG	-	chr1.1:50225450-50225469	None:intergenic	50.0%
!!	GCTCTTGGCTTTTTCTCTGC+TGG	-	chr1.1:50225162-50225181	None:intergenic	50.0%
!!!	AGCGGGTTTTTTCTCTGCCT+TGG	-	chr1.1:50225115-50225134	None:intergenic	50.0%
!!!	GCGGGTTTTTTCTCTGCCTT+GGG	-	chr1.1:50225114-50225133	None:intergenic	50.0%
	CGCTCAGGAAGCTTCCAGAT+TGG	+	chr1.1:50225359-50225378	MS.gene068529:CDS	55.0%
	CTCTGCTGGTGATTTCTCCG+CGG	-	chr1.1:50225148-50225167	None:intergenic	55.0%
	TCTGCTGGTGATTTCTCCGC+GGG	-	chr1.1:50225147-50225166	None:intergenic	55.0%
!!	GCTGTGCGTTTGGTTCTTCC+TGG	+	chr1.1:50225429-50225448	MS.gene068529:CDS	55.0%
	ACCCGCTGAGAAGAAACCCG+CGG	+	chr1.1:50225128-50225147	MS.gene068529:CDS	60.0%
	CTCCGCGGGTTTCTTCTCAG+CGG	-	chr1.1:50225133-50225152	None:intergenic	60.0%
	TCCGCGGGTTTCTTCTCAGC+GGG	-	chr1.1:50225132-50225151	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	50225105	50225515	50225105	ID=MS.gene068529
chr1.1	mRNA	50225105	50225515	50225105	ID=MS.gene068529.t1;Parent=MS.gene068529
chr1.1	exon	50225105	50225515	50225105	ID=MS.gene068529.t1.exon1;Parent=MS.gene068529.t1
chr1.1	CDS	50225105	50225515	50225105	ID=cds.MS.gene068529.t1;Parent=MS.gene068529.t1

Gene Sequence

>MS.gene068529

ATGGCTCCCAAGGCAGAGAAAAAACCCGCTGAGAAGAAACCCGCGGAGAAATCACCAGCAGAGAAAAAGCCAAGAGCCGAGAAGAAGATCTCGAAAGAAGGAGGCGATAAGAAGAAGAAGCGCGTGAAGAAGAGCGTTGAAACCTATAAGATTTACATTTTCAAGGTTCTCAAACAGGTTCATCCTGATATAGGTATTTCTTCAAAAGCTATGGGAATTATGAACAGTTTCATCAATGATATCTTTGAGAAACTCGCTCAGGAAGCTTCCAGATTGGCTCGTTACAACAAGAAACCGACGATTACGTCACGGGAGATTCAAACCGCTGTGCGTTTGGTTCTTCCTGGTGAATTGGCGAAACATGCTGTTTCTGAAGGAACTAAGGCTGTTACCAAATTTACGAGTTCTTGA

Protein sequence

>MS.gene068529.t1

MAPKAEKKPAEKKPAEKSPAEKKPRAEKKISKEGGDKKKKRVKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQEASRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS