Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071258.t1 | XP_003595673.1 | 94.2 | 670 | 33 | 2 | 1 | 664 | 1 | 670 | 0.00E+00 | 1213.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071258.t1 | Q9C9C0 | 67.7 | 678 | 196 | 6 | 4 | 664 | 5 | 676 | 7.1e-249 | 861.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071258.t1 | G7ISZ4 | 94.2 | 670 | 33 | 2 | 1 | 664 | 1 | 670 | 0.0e+00 | 1213.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene071258 | 0.824958 | 6.09E-54 | -1.69E-46 |
| MS.gene049356 | MS.gene071258 | 0.815896 | 7.31E-52 | -1.69E-46 |
| MS.gene049369 | MS.gene071258 | 0.820694 | 5.99E-53 | -1.69E-46 |
| MS.gene049427 | MS.gene071258 | 0.81893 | 1.52E-52 | -1.69E-46 |
| MS.gene049609 | MS.gene071258 | 0.819171 | 1.34E-52 | -1.69E-46 |
| MS.gene049615 | MS.gene071258 | 0.801112 | 1.05E-48 | -1.69E-46 |
| MS.gene050520 | MS.gene071258 | 0.83024 | 3.28E-55 | -1.69E-46 |
| MS.gene050538 | MS.gene071258 | 0.846156 | 2.59E-59 | -1.69E-46 |
| MS.gene050593 | MS.gene071258 | 0.860214 | 2.41E-63 | -1.69E-46 |
| MS.gene050670 | MS.gene071258 | 0.860726 | 1.69E-63 | -1.69E-46 |
| MS.gene050747 | MS.gene071258 | 0.857859 | 1.22E-62 | -1.69E-46 |
| MS.gene050772 | MS.gene071258 | 0.829414 | 5.22E-55 | -1.69E-46 |
| MS.gene050812 | MS.gene071258 | 0.834176 | 3.49E-56 | -1.69E-46 |
| MS.gene05081 | MS.gene071258 | 0.801961 | 7.03E-49 | -1.69E-46 |
| MS.gene050950 | MS.gene071258 | 0.858048 | 1.08E-62 | -1.69E-46 |
| MS.gene05105 | MS.gene071258 | 0.830703 | 2.53E-55 | -1.69E-46 |
| MS.gene051255 | MS.gene071258 | 0.818552 | 1.85E-52 | -1.69E-46 |
| MS.gene051360 | MS.gene071258 | 0.818376 | 2.02E-52 | -1.69E-46 |
| MS.gene051387 | MS.gene071258 | 0.877962 | 4.13E-69 | -1.69E-46 |
| MS.gene051525 | MS.gene071258 | 0.82951 | 4.94E-55 | -1.69E-46 |
| MS.gene051666 | MS.gene071258 | 0.829158 | 6.02E-55 | -1.69E-46 |
| MS.gene051734 | MS.gene071258 | 0.817305 | 3.53E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene071258 | 0.826284 | 2.95E-54 | -1.69E-46 |
| MS.gene051941 | MS.gene071258 | 0.806541 | 7.82E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene071258 | 0.804544 | 2.05E-49 | -1.69E-46 |
| MS.gene052346 | MS.gene071258 | 0.830368 | 3.06E-55 | -1.69E-46 |
| MS.gene052414 | MS.gene071258 | 0.831146 | 1.97E-55 | -1.69E-46 |
| MS.gene052421 | MS.gene071258 | 0.812511 | 4.09E-51 | -1.69E-46 |
| MS.gene052481 | MS.gene071258 | 0.805308 | 1.42E-49 | -1.69E-46 |
| MS.gene052484 | MS.gene071258 | 0.801494 | 8.77E-49 | -1.69E-46 |
| MS.gene052485 | MS.gene071258 | 0.836686 | 8.09E-57 | -1.69E-46 |
| MS.gene052501 | MS.gene071258 | 0.826291 | 2.94E-54 | -1.69E-46 |
| MS.gene052503 | MS.gene071258 | 0.800837 | 1.19E-48 | -1.69E-46 |
| MS.gene052505 | MS.gene071258 | 0.814936 | 1.20E-51 | -1.69E-46 |
| MS.gene052615 | MS.gene071258 | 0.80154 | 8.58E-49 | -1.69E-46 |
| MS.gene052870 | MS.gene071258 | 0.825223 | 5.27E-54 | -1.69E-46 |
| MS.gene052872 | MS.gene071258 | 0.821303 | 4.34E-53 | -1.69E-46 |
| MS.gene052966 | MS.gene071258 | 0.809356 | 1.97E-50 | -1.69E-46 |
| MS.gene052982 | MS.gene071258 | 0.828655 | 7.97E-55 | -1.69E-46 |
| MS.gene053104 | MS.gene071258 | 0.814303 | 1.65E-51 | -1.69E-46 |
| MS.gene053107 | MS.gene071258 | 0.870834 | 1.08E-66 | -1.69E-46 |
| MS.gene053169 | MS.gene071258 | 0.846656 | 1.89E-59 | -1.69E-46 |
| MS.gene053207 | MS.gene071258 | 0.821804 | 3.32E-53 | -1.69E-46 |
| MS.gene053213 | MS.gene071258 | 0.820287 | 7.43E-53 | -1.69E-46 |
| MS.gene053286 | MS.gene071258 | 0.815536 | 8.79E-52 | -1.69E-46 |
| MS.gene053436 | MS.gene071258 | 0.824623 | 7.30E-54 | -1.69E-46 |
| MS.gene053449 | MS.gene071258 | 0.802033 | 6.80E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene071258 | 0.825447 | 4.66E-54 | -1.69E-46 |
| MS.gene053574 | MS.gene071258 | 0.843621 | 1.25E-58 | -1.69E-46 |
| MS.gene053824 | MS.gene071258 | 0.832926 | 7.15E-56 | -1.69E-46 |
| MS.gene054408 | MS.gene071258 | 0.816335 | 5.83E-52 | -1.69E-46 |
| MS.gene054556 | MS.gene071258 | 0.818794 | 1.63E-52 | -1.69E-46 |
| MS.gene054600 | MS.gene071258 | 0.815253 | 1.02E-51 | -1.69E-46 |
| MS.gene05494 | MS.gene071258 | 0.808371 | 3.20E-50 | -1.69E-46 |
| MS.gene05505 | MS.gene071258 | 0.817892 | 2.60E-52 | -1.69E-46 |
| MS.gene055116 | MS.gene071258 | 0.801939 | 7.10E-49 | -1.69E-46 |
| MS.gene055224 | MS.gene071258 | 0.858287 | 9.14E-63 | -1.69E-46 |
| MS.gene055369 | MS.gene071258 | 0.853203 | 2.79E-61 | -1.69E-46 |
| MS.gene055412 | MS.gene071258 | 0.806244 | 9.04E-50 | -1.69E-46 |
| MS.gene055539 | MS.gene071258 | 0.836492 | 9.06E-57 | -1.69E-46 |
| MS.gene055577 | MS.gene071258 | 0.839559 | 1.47E-57 | -1.69E-46 |
| MS.gene055620 | MS.gene071258 | 0.816035 | 6.81E-52 | -1.69E-46 |
| MS.gene055647 | MS.gene071258 | 0.803055 | 4.18E-49 | -1.69E-46 |
| MS.gene055722 | MS.gene071258 | 0.803574 | 3.27E-49 | -1.69E-46 |
| MS.gene055741 | MS.gene071258 | 0.838713 | 2.44E-57 | -1.69E-46 |
| MS.gene055744 | MS.gene071258 | 0.837247 | 5.82E-57 | -1.69E-46 |
| MS.gene055909 | MS.gene071258 | 0.830178 | 3.40E-55 | -1.69E-46 |
| MS.gene055911 | MS.gene071258 | 0.835133 | 2.00E-56 | -1.69E-46 |
| MS.gene05592 | MS.gene071258 | 0.801942 | 7.10E-49 | -1.69E-46 |
| MS.gene055941 | MS.gene071258 | 0.841004 | 6.18E-58 | -1.69E-46 |
| MS.gene056166 | MS.gene071258 | 0.820201 | 7.77E-53 | -1.69E-46 |
| MS.gene056407 | MS.gene071258 | 0.838614 | 2.59E-57 | -1.69E-46 |
| MS.gene056410 | MS.gene071258 | 0.802233 | 6.18E-49 | -1.69E-46 |
| MS.gene056422 | MS.gene071258 | 0.802569 | 5.27E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene071258 | 0.820517 | 6.58E-53 | -1.69E-46 |
| MS.gene05664 | MS.gene071258 | 0.819141 | 1.36E-52 | -1.69E-46 |
| MS.gene05666 | MS.gene071258 | 0.843862 | 1.08E-58 | -1.69E-46 |
| MS.gene056796 | MS.gene071258 | 0.815003 | 1.16E-51 | -1.69E-46 |
| MS.gene056877 | MS.gene071258 | 0.849797 | 2.57E-60 | -1.69E-46 |
| MS.gene056897 | MS.gene071258 | 0.809945 | 1.47E-50 | -1.69E-46 |
| MS.gene056947 | MS.gene071258 | 0.842795 | 2.08E-58 | -1.69E-46 |
| MS.gene057011 | MS.gene071258 | 0.83298 | 6.94E-56 | -1.69E-46 |
| MS.gene057019 | MS.gene071258 | 0.833765 | 4.42E-56 | -1.69E-46 |
| MS.gene057076 | MS.gene071258 | 0.806228 | 9.11E-50 | -1.69E-46 |
| MS.gene057124 | MS.gene071258 | 0.828206 | 1.02E-54 | -1.69E-46 |
| MS.gene057331 | MS.gene071258 | 0.855233 | 7.24E-62 | -1.69E-46 |
| MS.gene057332 | MS.gene071258 | 0.821335 | 4.26E-53 | -1.69E-46 |
| MS.gene057416 | MS.gene071258 | 0.82494 | 6.15E-54 | -1.69E-46 |
| MS.gene057658 | MS.gene071258 | 0.804355 | 2.25E-49 | -1.69E-46 |
| MS.gene057734 | MS.gene071258 | 0.805223 | 1.48E-49 | -1.69E-46 |
| MS.gene058007 | MS.gene071258 | 0.820134 | 8.05E-53 | -1.69E-46 |
| MS.gene058019 | MS.gene071258 | 0.827511 | 1.50E-54 | -1.69E-46 |
| MS.gene058120 | MS.gene071258 | 0.811677 | 6.21E-51 | -1.69E-46 |
| MS.gene058130 | MS.gene071258 | 0.811031 | 8.58E-51 | -1.69E-46 |
| MS.gene058293 | MS.gene071258 | 0.808687 | 2.74E-50 | -1.69E-46 |
| MS.gene058323 | MS.gene071258 | 0.83858 | 2.64E-57 | -1.69E-46 |
| MS.gene058365 | MS.gene071258 | 0.809019 | 2.33E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene071258 | 0.848883 | 4.61E-60 | -1.69E-46 |
| MS.gene058444 | MS.gene071258 | 0.820825 | 5.59E-53 | -1.69E-46 |
| MS.gene058700 | MS.gene071258 | 0.886175 | 4.30E-72 | -1.69E-46 |
| MS.gene058838 | MS.gene071258 | 0.806943 | 6.44E-50 | -1.69E-46 |
| MS.gene058948 | MS.gene071258 | 0.860445 | 2.06E-63 | -1.69E-46 |
| MS.gene059023 | MS.gene071258 | 0.827965 | 1.17E-54 | -1.69E-46 |
| MS.gene05907 | MS.gene071258 | 0.803895 | 2.80E-49 | -1.69E-46 |
| MS.gene059305 | MS.gene071258 | 0.806396 | 8.40E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene071258 | 0.838011 | 3.70E-57 | -1.69E-46 |
| MS.gene059420 | MS.gene071258 | 0.819542 | 1.10E-52 | -1.69E-46 |
| MS.gene059676 | MS.gene071258 | 0.822223 | 2.66E-53 | -1.69E-46 |
| MS.gene059680 | MS.gene071258 | 0.803055 | 4.18E-49 | -1.69E-46 |
| MS.gene059688 | MS.gene071258 | 0.816615 | 5.05E-52 | -1.69E-46 |
| MS.gene059692 | MS.gene071258 | 0.801301 | 9.61E-49 | -1.69E-46 |
| MS.gene059695 | MS.gene071258 | 0.810144 | 1.33E-50 | -1.69E-46 |
| MS.gene059960 | MS.gene071258 | 0.801505 | 8.73E-49 | -1.69E-46 |
| MS.gene060193 | MS.gene071258 | 0.842477 | 2.52E-58 | -1.69E-46 |
| MS.gene060403 | MS.gene071258 | 0.810551 | 1.09E-50 | -1.69E-46 |
| MS.gene060804 | MS.gene071258 | 0.844829 | 5.93E-59 | -1.69E-46 |
| MS.gene060835 | MS.gene071258 | 0.852622 | 4.09E-61 | -1.69E-46 |
| MS.gene060837 | MS.gene071258 | 0.855156 | 7.62E-62 | -1.69E-46 |
| MS.gene06092 | MS.gene071258 | 0.854876 | 9.19E-62 | -1.69E-46 |
| MS.gene061051 | MS.gene071258 | -0.80988 | 1.52E-50 | -1.69E-46 |
| MS.gene061154 | MS.gene071258 | 0.856884 | 2.38E-62 | -1.69E-46 |
| MS.gene061200 | MS.gene071258 | 0.811156 | 8.06E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071258.t1 | MTR_2g058970 | 94.925 | 670 | 28 | 2 | 1 | 664 | 1 | 670 | 0.0 | 1229 |
| MS.gene071258.t1 | MTR_4g112860 | 78.983 | 590 | 123 | 1 | 75 | 663 | 68 | 657 | 0.0 | 969 |
| MS.gene071258.t1 | MTR_4g112860 | 78.419 | 468 | 100 | 1 | 75 | 541 | 68 | 535 | 0.0 | 770 |
| MS.gene071258.t1 | MTR_4g112860 | 76.980 | 404 | 92 | 1 | 75 | 477 | 68 | 471 | 0.0 | 657 |
| MS.gene071258.t1 | MTR_4g112820 | 60.385 | 520 | 126 | 5 | 70 | 589 | 41 | 480 | 0.0 | 586 |
| MS.gene071258.t1 | MTR_4g112860 | 72.590 | 332 | 90 | 1 | 75 | 405 | 68 | 399 | 0.0 | 521 |
| MS.gene071258.t1 | MTR_5g025810 | 41.561 | 551 | 169 | 11 | 77 | 624 | 34 | 434 | 3.70e-116 | 358 |
| MS.gene071258.t1 | MTR_4g114810 | 42.780 | 554 | 155 | 17 | 98 | 638 | 65 | 469 | 8.21e-110 | 340 |
| MS.gene071258.t1 | MTR_4g134260 | 51.685 | 356 | 104 | 6 | 303 | 658 | 135 | 422 | 1.89e-99 | 311 |
| MS.gene071258.t1 | MTR_4g134260 | 66.667 | 111 | 36 | 1 | 122 | 231 | 27 | 137 | 9.28e-43 | 160 |
| MS.gene071258.t1 | MTR_4g130360 | 42.697 | 445 | 150 | 10 | 74 | 493 | 75 | 439 | 1.44e-97 | 308 |
| MS.gene071258.t1 | MTR_4g130360 | 74.603 | 63 | 14 | 1 | 542 | 602 | 390 | 452 | 1.23e-20 | 95.9 |
| MS.gene071258.t1 | MTR_4g123500 | 40.125 | 481 | 236 | 12 | 136 | 605 | 11 | 450 | 8.51e-94 | 298 |
| MS.gene071258.t1 | MTR_8g022470 | 50.000 | 370 | 128 | 10 | 232 | 598 | 1 | 316 | 8.44e-91 | 285 |
| MS.gene071258.t1 | MTR_5g082990 | 47.090 | 378 | 119 | 11 | 196 | 566 | 6 | 309 | 1.13e-89 | 284 |
| MS.gene071258.t1 | MTR_8g022500 | 62.844 | 218 | 70 | 5 | 387 | 601 | 222 | 431 | 1.79e-69 | 235 |
| MS.gene071258.t1 | MTR_8g022500 | 34.595 | 185 | 67 | 4 | 74 | 257 | 56 | 187 | 6.71e-21 | 97.1 |
| MS.gene071258.t1 | MTR_4g133130 | 48.207 | 251 | 64 | 6 | 353 | 597 | 37 | 227 | 4.85e-51 | 186 |
| MS.gene071258.t1 | MTR_5g083090 | 44.920 | 187 | 72 | 7 | 102 | 285 | 1 | 159 | 6.01e-32 | 122 |
| MS.gene071258.t1 | MTR_5g083100 | 55.639 | 133 | 42 | 4 | 284 | 414 | 37 | 154 | 2.18e-31 | 128 |
| MS.gene071258.t1 | MTR_5g083100 | 68.657 | 67 | 18 | 1 | 531 | 594 | 177 | 243 | 5.40e-20 | 93.6 |
| MS.gene071258.t1 | MTR_4g130550 | 37.853 | 177 | 102 | 2 | 390 | 566 | 65 | 233 | 5.03e-31 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071258.t1 | AT1G73990 | 68.627 | 663 | 183 | 6 | 11 | 664 | 30 | 676 | 0.0 | 904 |
| MS.gene071258.t1 | AT1G73990 | 69.074 | 540 | 142 | 6 | 11 | 541 | 30 | 553 | 0.0 | 747 |
Find 156 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTATGATCCTCGAATTTC+TGG | 0.156422 | 2.1:-35510659 | MS.gene071258:CDS |
| AAATGGACAGTTGGAATTTC+AGG | 0.181698 | 2.1:-35506574 | MS.gene071258:CDS |
| CATACATTAAATTTGATATA+AGG | 0.278743 | 2.1:+35507929 | None:intergenic |
| GCAGAAAGTCTTACCTTAAC+TGG | 0.282389 | 2.1:-35505019 | MS.gene071258:CDS |
| TTTGAATTCGAACCTATTAC+TGG | 0.295958 | 2.1:-35511837 | MS.gene071258:CDS |
| ATATGCATTCTGTGCAGATT+TGG | 0.331313 | 2.1:+35502268 | None:intergenic |
| AACTGCCAACGGTCGATTAC+AGG | 0.347139 | 2.1:-35507515 | MS.gene071258:intron |
| AGGTAGCACAGGGGAGAGTT+TGG | 0.351330 | 2.1:-35502092 | MS.gene071258:CDS |
| CATGGTTTGGTTGATGCTAT+TGG | 0.357229 | 2.1:-35502048 | MS.gene071258:CDS |
| GGTGTTGCGGCGGTAGAGGG+GGG | 0.359266 | 2.1:+35512061 | None:intergenic |
| GCAAATAGCTCTGCTTCATC+TGG | 0.360163 | 2.1:+35502290 | None:intergenic |
| AGGTGTTGCGGCGGTAGAGG+GGG | 0.360641 | 2.1:+35512060 | None:intergenic |
| GACAAAATGGGGTTGTCGTT+GGG | 0.368893 | 2.1:+35501695 | None:intergenic |
| ACGTGCTTTCAACTCCGGTT+AGG | 0.374016 | 2.1:+35501804 | None:intergenic |
| AAATTCCTCCAACCAGTAAT+AGG | 0.377007 | 2.1:+35511825 | None:intergenic |
| CACAGTTTAAAGTATCAATA+TGG | 0.378673 | 2.1:+35510627 | None:intergenic |
| CTTACCTTAACTGGTTCAAT+TGG | 0.381447 | 2.1:-35505010 | MS.gene071258:CDS |
| AAGGTGTTGCGGCGGTAGAG+GGG | 0.381937 | 2.1:+35512059 | None:intergenic |
| AAAACCAGTCATTGCTTCAA+TGG | 0.389485 | 2.1:-35505098 | MS.gene071258:CDS |
| ATAGCCATCATCCGGGCCTC+AGG | 0.402955 | 2.1:-35506538 | MS.gene071258:CDS |
| TACAATGAACCTTCGTGGCC+AGG | 0.408769 | 2.1:-35511739 | MS.gene071258:intron |
| AGGGAGCATTAGTCGAGTTA+AGG | 0.410229 | 2.1:-35506518 | MS.gene071258:CDS |
| CTGTTCGTCCTGAGGTATAT+TGG | 0.410992 | 2.1:+35501987 | None:intergenic |
| CACTCCAATTGAACCAGTTA+AGG | 0.411932 | 2.1:+35505006 | None:intergenic |
| TTGGAGGAATTTCGTCGTTA+AGG | 0.417101 | 2.1:-35511814 | MS.gene071258:CDS |
| ATTGAGAACTTTATAAATGA+AGG | 0.419440 | 2.1:-35507985 | MS.gene071258:CDS |
| ACAGGAAATACTCCCGGGTT+AGG | 0.423190 | 2.1:-35506597 | MS.gene071258:intron |
| AGATGCAGCATCACATGGTT+TGG | 0.425370 | 2.1:-35502061 | MS.gene071258:CDS |
| CGTGCTTTCAACTCCGGTTA+GGG | 0.429338 | 2.1:+35501805 | None:intergenic |
| AATACTCCCGGGTTAGGAAA+TGG | 0.431438 | 2.1:-35506591 | MS.gene071258:CDS |
| CACGTTGAAGGAACTGCTAC+AGG | 0.432578 | 2.1:-35501786 | MS.gene071258:CDS |
| CCTATGATCCTCGAATTTCT+GGG | 0.434964 | 2.1:-35510658 | MS.gene071258:CDS |
| AGGTAGAGAGGGACTAGATC+TGG | 0.444443 | 2.1:+35501853 | None:intergenic |
| ATAAGGTGTTGCGGCGGTAG+AGG | 0.455950 | 2.1:+35512057 | None:intergenic |
| TTCGAATTCAAATTCTCCAC+TGG | 0.457392 | 2.1:+35511848 | None:intergenic |
| CAATATACCTCAGGACGAAC+AGG | 0.460640 | 2.1:-35501986 | MS.gene071258:intron |
| AAGAACTGATAGCCATCATC+CGG | 0.468277 | 2.1:-35506546 | MS.gene071258:CDS |
| CCCAGAAATTCGAGGATCAT+AGG | 0.470601 | 2.1:+35510658 | None:intergenic |
| TAGATAATATCTATACAAAC+TGG | 0.471417 | 2.1:-35508125 | MS.gene071258:CDS |
| GGGAGCATTAGTCGAGTTAA+GGG | 0.472364 | 2.1:-35506517 | MS.gene071258:CDS |
| TTAGGATGTGGAGAGAAATT+CGG | 0.477457 | 2.1:-35505135 | MS.gene071258:intron |
| GAGGCAAGAGCGTCACCTCC+AGG | 0.482340 | 2.1:+35505816 | None:intergenic |
| TTAAAATCTACAGGGAACTA+AGG | 0.483269 | 2.1:+35501657 | None:intergenic |
| TGACAAAATGGGGTTGTCGT+TGG | 0.483685 | 2.1:+35501694 | None:intergenic |
| GCAGGTTACTGACATGTTGA+AGG | 0.485150 | 2.1:-35507565 | MS.gene071258:intron |
| AGATGCTCATTGCGTATCCA+TGG | 0.485437 | 2.1:-35511788 | MS.gene071258:CDS |
| GTGTTGCGGCGGTAGAGGGG+GGG | 0.488093 | 2.1:+35512062 | None:intergenic |
| GGCGCACGGAGTTCCCTAAC+CGG | 0.489745 | 2.1:-35501818 | MS.gene071258:CDS |
| CAATTAAGTGTCTCTAGCTC+AGG | 0.490023 | 2.1:-35506493 | MS.gene071258:CDS |
| TAAGGTGTTGCGGCGGTAGA+GGG | 0.494514 | 2.1:+35512058 | None:intergenic |
| GACTAATGCTCCCTGAGGCC+CGG | 0.494942 | 2.1:+35506527 | None:intergenic |
| CAACTGTCCATTTCCTAACC+CGG | 0.498705 | 2.1:+35506584 | None:intergenic |
| TTAATAAAGAAATCATATCA+AGG | 0.499995 | 2.1:-35503506 | MS.gene071258:CDS |
| CTTCAACGTGCTTTCAACTC+CGG | 0.501193 | 2.1:+35501799 | None:intergenic |
| CGATGGAGTTCAAGCTAGGA+TGG | 0.505001 | 2.1:-35501750 | MS.gene071258:CDS |
| GCTCCCTGAGGCCCGGATGA+TGG | 0.505280 | 2.1:+35506534 | None:intergenic |
| GCGAAACTGTATGAGAAGAT+TGG | 0.507637 | 2.1:-35503531 | MS.gene071258:CDS |
| CAAGATGGAGGAGGTAGCAC+AGG | 0.509168 | 2.1:-35502103 | MS.gene071258:CDS |
| TTGCCAATCCTTTCCACTTG+CGG | 0.509346 | 2.1:+35508222 | None:intergenic |
| GTTCTTACAATGAACCTTCG+TGG | 0.510345 | 2.1:-35511744 | MS.gene071258:CDS |
| CGTGCGCCTAATAAAATCTC+AGG | 0.511141 | 2.1:+35501833 | None:intergenic |
| GTGAAGAGTGGATTCTCTCC+GGG | 0.511809 | 2.1:-35510722 | MS.gene071258:CDS |
| TTTCCGATGGAGTTCAAGCT+AGG | 0.515610 | 2.1:-35501754 | MS.gene071258:CDS |
| AGTGAAGAGTGGATTCTCTC+CGG | 0.516240 | 2.1:-35510723 | MS.gene071258:CDS |
| AGAACATACAGTCATAGATC+TGG | 0.517426 | 2.1:+35502217 | None:intergenic |
| GGAGGATACTACATGGCAAT+GGG | 0.520535 | 2.1:-35505058 | MS.gene071258:CDS |
| TATTGCCATAGCAAAATTGA+AGG | 0.523316 | 2.1:-35502010 | MS.gene071258:CDS |
| GGAAGAATCAAAAGCGCGAA+GGG | 0.529032 | 2.1:+35511941 | None:intergenic |
| ATTATCAGAATTGACAGTCC+TGG | 0.529856 | 2.1:-35505834 | MS.gene071258:CDS |
| TCGAACCTATTACTGGTTGG+AGG | 0.530867 | 2.1:-35511830 | MS.gene071258:CDS |
| GATACTTTAAACTGTGGGTG+GGG | 0.532084 | 2.1:-35510620 | MS.gene071258:CDS |
| GGTTTGGTTGATGCTATTGG+TGG | 0.533991 | 2.1:-35502045 | MS.gene071258:CDS |
| TAATATCTATACAAACTGGC+TGG | 0.538238 | 2.1:-35508121 | MS.gene071258:CDS |
| CGGAGTTGAAAGCACGTTGA+AGG | 0.539872 | 2.1:-35501798 | MS.gene071258:CDS |
| TGATACTTTAAACTGTGGGT+GGG | 0.540969 | 2.1:-35510621 | MS.gene071258:CDS |
| ATTGGCAAGTACAAAAGTGC+AGG | 0.541412 | 2.1:-35508207 | MS.gene071258:CDS |
| GGTTCAATTGGAGTGGTCAC+AGG | 0.541619 | 2.1:-35504998 | MS.gene071258:intron |
| AAAGAAAAGTAAGCACTTGG+AGG | 0.543239 | 2.1:+35509718 | None:intergenic |
| TTAAATACAGGAAATACTCC+CGG | 0.544002 | 2.1:-35506603 | MS.gene071258:intron |
| TTGATACTTTAAACTGTGGG+TGG | 0.545472 | 2.1:-35510622 | MS.gene071258:CDS |
| AATTCGAACCTATTACTGGT+TGG | 0.546364 | 2.1:-35511833 | MS.gene071258:CDS |
| GAACCGCAAGTGGAAAGGAT+TGG | 0.547009 | 2.1:-35508225 | MS.gene071258:CDS |
| AACTGTCCATTTCCTAACCC+GGG | 0.547411 | 2.1:+35506585 | None:intergenic |
| CTCTACCTAGGTCGACAAGA+TGG | 0.547751 | 2.1:-35502118 | MS.gene071258:intron |
| TCTTCTCATACAGTTTCGCT+AGG | 0.547877 | 2.1:+35503535 | None:intergenic |
| AACAAAGAAAAGTAAGCACT+TGG | 0.548235 | 2.1:+35509715 | None:intergenic |
| TAAATACAGGAAATACTCCC+GGG | 0.550900 | 2.1:-35506602 | MS.gene071258:intron |
| TTCTACCTGTAATCGACCGT+TGG | 0.551223 | 2.1:+35507510 | None:intergenic |
| CCTTTACGAACACGTTCCCA+TGG | 0.553283 | 2.1:+35511771 | None:intergenic |
| ATTGAAGGCCAATATACCTC+AGG | 0.554095 | 2.1:-35501995 | MS.gene071258:CDS |
| CCATGGGAACGTGTTCGTAA+AGG | 0.554425 | 2.1:-35511771 | MS.gene071258:CDS |
| CTTAACTGGTTCAATTGGAG+TGG | 0.555659 | 2.1:-35505005 | MS.gene071258:CDS |
| GGAGTTCAAGCTAGGATGGA+TGG | 0.557065 | 2.1:-35501746 | MS.gene071258:CDS |
| AATAAAATCTCAGGTAGAGA+GGG | 0.563178 | 2.1:+35501842 | None:intergenic |
| TCAGCCATTGAAGCAATGAC+TGG | 0.563949 | 2.1:+35505094 | None:intergenic |
| TAATAAAGAAATCATATCAA+GGG | 0.565337 | 2.1:-35503505 | MS.gene071258:CDS |
| AAATTTAATGTATGATGATG+AGG | 0.565513 | 2.1:-35507920 | MS.gene071258:intron |
| TGGAGGATACTACATGGCAA+TGG | 0.566417 | 2.1:-35505059 | MS.gene071258:CDS |
| ATATTGATACTTTAAACTGT+GGG | 0.566491 | 2.1:-35510625 | MS.gene071258:CDS |
| TACCAGAACTTACAAATCAG+AGG | 0.568309 | 2.1:+35505797 | None:intergenic |
| AATAGGAGTTGAACCGCAAG+TGG | 0.570611 | 2.1:-35508235 | MS.gene071258:CDS |
| CAGCTTACCTCTGAGGAATG+AGG | 0.572547 | 2.1:+35509675 | None:intergenic |
| CATTGCTTCAATGGCTGATG+TGG | 0.572578 | 2.1:-35505089 | MS.gene071258:CDS |
| AAGAAAAGTAAGCACTTGGA+GGG | 0.573901 | 2.1:+35509719 | None:intergenic |
| TCGAATTCAAATTCTCCACT+GGG | 0.574482 | 2.1:+35511849 | None:intergenic |
| CATCCTAGCTTGAACTCCAT+CGG | 0.576476 | 2.1:+35501751 | None:intergenic |
| GAGTTGAACCGCAAGTGGAA+AGG | 0.579458 | 2.1:-35508230 | MS.gene071258:CDS |
| TAGCCATCATCCGGGCCTCA+GGG | 0.580655 | 2.1:-35506537 | MS.gene071258:CDS |
| CGGGTTAGGAAATGGACAGT+TGG | 0.581781 | 2.1:-35506583 | MS.gene071258:CDS |
| CATATTGATACTTTAAACTG+TGG | 0.585266 | 2.1:-35510626 | MS.gene071258:CDS |
| CGTTGAAGGAACTGCTACAG+GGG | 0.585808 | 2.1:-35501784 | MS.gene071258:CDS |
| CTATGGCAATAGCTCGGGAA+AGG | 0.585943 | 2.1:+35502022 | None:intergenic |
| TAATAAAATCTCAGGTAGAG+AGG | 0.589084 | 2.1:+35501841 | None:intergenic |
| GATGCTCATTGCGTATCCAT+GGG | 0.597452 | 2.1:-35511787 | MS.gene071258:CDS |
| GTTGATAAGCTGAAAGAAGA+GGG | 0.598500 | 2.1:-35507952 | MS.gene071258:CDS |
| AGATAGACCCCAGAAATTCG+AGG | 0.602106 | 2.1:+35510650 | None:intergenic |
| ATGGCTGATGTGGCAGCAAG+TGG | 0.602616 | 2.1:-35505079 | MS.gene071258:CDS |
| GATAAAGTCTCTTCTGCCAA+AGG | 0.606342 | 2.1:-35508099 | MS.gene071258:intron |
| GAAAGACCAGCTTACCTCTG+AGG | 0.608420 | 2.1:+35509668 | None:intergenic |
| AAGATGGAGGAGGTAGCACA+GGG | 0.610247 | 2.1:-35502102 | MS.gene071258:CDS |
| CTAGGTCGACAAGATGGAGG+AGG | 0.611196 | 2.1:-35502112 | MS.gene071258:intron |
| GACATGTTGAAGGAGAGACT+TGG | 0.612900 | 2.1:-35507555 | MS.gene071258:CDS |
| GAGAAGATTCGTACTGTCAG+AGG | 0.617688 | 2.1:-35506448 | MS.gene071258:intron |
| ACGTTGAAGGAACTGCTACA+GGG | 0.618985 | 2.1:-35501785 | MS.gene071258:CDS |
| AGTTGATAAGCTGAAAGAAG+AGG | 0.622772 | 2.1:-35507953 | MS.gene071258:CDS |
| AATAAAGAAATCATATCAAG+GGG | 0.623523 | 2.1:-35503504 | MS.gene071258:CDS |
| TAACTAACCTGTTCGTCCTG+AGG | 0.625627 | 2.1:+35501979 | None:intergenic |
| TGGACAGTTGGAATTTCAGG+TGG | 0.627222 | 2.1:-35506571 | MS.gene071258:CDS |
| GGTAAAGATGCAGCATCACA+TGG | 0.630228 | 2.1:-35502066 | MS.gene071258:CDS |
| AGGAAGAATCAAAAGCGCGA+AGG | 0.632352 | 2.1:+35511940 | None:intergenic |
| GCTTGTAGGAAAATTTGTCG+TGG | 0.632736 | 2.1:-35509806 | MS.gene071258:intron |
| AGAACTGATAGCCATCATCC+GGG | 0.633122 | 2.1:-35506545 | MS.gene071258:CDS |
| TAGCTCAGGCATCACTGCGG+AGG | 0.634924 | 2.1:-35506479 | MS.gene071258:CDS |
| ATATCTGATCAAGTGAAGAG+TGG | 0.635175 | 2.1:-35510734 | MS.gene071258:CDS |
| AGAAAAGTAAGCACTTGGAG+GGG | 0.636043 | 2.1:+35509720 | None:intergenic |
| GTAAAGTAGAAGAAATTCGA+AGG | 0.637376 | 2.1:-35510598 | MS.gene071258:CDS |
| AGCAAGTGGAGGATACTACA+TGG | 0.638303 | 2.1:-35505065 | MS.gene071258:CDS |
| TACCTAGGTCGACAAGATGG+AGG | 0.644757 | 2.1:-35502115 | MS.gene071258:intron |
| GCTATGCCAGGTAACTGTTG+TGG | 0.645235 | 2.1:-35501882 | MS.gene071258:intron |
| CTATGATCCTCGAATTTCTG+GGG | 0.650704 | 2.1:-35510657 | MS.gene071258:CDS |
| ATTTGAGGTAGAGAAAGTCC+CGG | 0.652101 | 2.1:+35510704 | None:intergenic |
| AGAATGAAACCTGGCCACGA+AGG | 0.657224 | 2.1:+35511730 | None:intergenic |
| ATGAATTATTAAAATCTACA+GGG | 0.660702 | 2.1:+35501649 | None:intergenic |
| GCACAGGGGAGAGTTTGGAC+TGG | 0.665356 | 2.1:-35502087 | MS.gene071258:CDS |
| GCTGATGTGGCAGCAAGTGG+AGG | 0.665663 | 2.1:-35505076 | MS.gene071258:CDS |
| TCTCTTCACATGCACATGCG+AGG | 0.666552 | 2.1:+35509749 | None:intergenic |
| ATCAGAATTGACAGTCCTGG+AGG | 0.668918 | 2.1:-35505831 | MS.gene071258:CDS |
| ATTAGCTCGAACAACCATGT+CGG | 0.671002 | 2.1:-35508178 | MS.gene071258:CDS |
| ATATAAACAATTTCGAGACA+AGG | 0.676264 | 2.1:-35502249 | MS.gene071258:CDS |
| ATGATGATGATAAGGTGTTG+CGG | 0.697257 | 2.1:+35512048 | None:intergenic |
| GCTGATGAAGATTATCCCAG+TGG | 0.718921 | 2.1:-35511864 | MS.gene071258:CDS |
| GTTCAAGCCTCATTCCTCAG+AGG | 0.722696 | 2.1:-35509682 | MS.gene071258:intron |
| AACTCGACTAATGCTCCCTG+AGG | 0.724123 | 2.1:+35506522 | None:intergenic |
| CTCTAGCTCAGGCATCACTG+CGG | 0.730860 | 2.1:-35506482 | MS.gene071258:CDS |
| AGATGGAGGAGGTAGCACAG+GGG | 0.746624 | 2.1:-35502101 | MS.gene071258:CDS |
| ATGATGATAAGGTGTTGCGG+CGG | 0.786339 | 2.1:+35512051 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 35501664 | 35512108 | 35501664 | ID=MS.gene071258 |
| chr2.1 | mRNA | 35501664 | 35512108 | 35501664 | ID=MS.gene071258.t1;Parent=MS.gene071258 |
| chr2.1 | exon | 35511740 | 35512108 | 35511740 | ID=MS.gene071258.t1.exon1;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35511740 | 35512108 | 35511740 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| chr2.1 | exon | 35510573 | 35510756 | 35510573 | ID=MS.gene071258.t1.exon2;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35510573 | 35510756 | 35510573 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| chr2.1 | exon | 35509683 | 35509820 | 35509683 | ID=MS.gene071258.t1.exon3;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35509683 | 35509820 | 35509683 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| chr2.1 | exon | 35508100 | 35508270 | 35508100 | ID=MS.gene071258.t1.exon4;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35508100 | 35508270 | 35508100 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| chr2.1 | exon | 35507921 | 35508024 | 35507921 | ID=MS.gene071258.t1.exon5;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35507921 | 35508024 | 35507921 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| chr2.1 | exon | 35507516 | 35507583 | 35507516 | ID=MS.gene071258.t1.exon6;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35507516 | 35507583 | 35507516 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| chr2.1 | exon | 35506449 | 35506615 | 35506449 | ID=MS.gene071258.t1.exon7;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35506449 | 35506615 | 35506449 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| chr2.1 | exon | 35505810 | 35505879 | 35505810 | ID=MS.gene071258.t1.exon8;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35505810 | 35505879 | 35505810 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| chr2.1 | exon | 35504999 | 35505153 | 35504999 | ID=MS.gene071258.t1.exon9;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35504999 | 35505153 | 35504999 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| chr2.1 | exon | 35503462 | 35503567 | 35503462 | ID=MS.gene071258.t1.exon10;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35503462 | 35503567 | 35503462 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| chr2.1 | exon | 35502226 | 35502313 | 35502226 | ID=MS.gene071258.t1.exon11;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35502226 | 35502313 | 35502226 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| chr2.1 | exon | 35501987 | 35502130 | 35501987 | ID=MS.gene071258.t1.exon12;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35501987 | 35502130 | 35501987 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| chr2.1 | exon | 35501664 | 35501894 | 35501664 | ID=MS.gene071258.t1.exon13;Parent=MS.gene071258.t1 |
| chr2.1 | CDS | 35501664 | 35501894 | 35501664 | ID=cds.MS.gene071258.t1;Parent=MS.gene071258.t1 |
| Gene Sequence |
| Protein sequence |
