Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37952.t1 | RHN58005.1 | 100 | 73 | 0 | 0 | 1 | 73 | 121 | 193 | 2.60E-31 | 144.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37952.t1 | Q9FM67 | 80.8 | 73 | 14 | 0 | 1 | 73 | 137 | 209 | 2.2e-26 | 119.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37952.t1 | G7KEF3 | 100.0 | 73 | 0 | 0 | 1 | 73 | 143 | 215 | 1.9e-31 | 144.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene37952 | 0.835457 | 1.66E-56 | -1.69E-46 |
| MS.gene049151 | MS.gene37952 | 0.805359 | 1.39E-49 | -1.69E-46 |
| MS.gene049153 | MS.gene37952 | 0.840804 | 6.97E-58 | -1.69E-46 |
| MS.gene049260 | MS.gene37952 | 0.820904 | 5.36E-53 | -1.69E-46 |
| MS.gene049284 | MS.gene37952 | 0.813168 | 2.93E-51 | -1.69E-46 |
| MS.gene049297 | MS.gene37952 | 0.827911 | 1.20E-54 | -1.69E-46 |
| MS.gene049346 | MS.gene37952 | 0.826529 | 2.58E-54 | -1.69E-46 |
| MS.gene049351 | MS.gene37952 | 0.815339 | 9.72E-52 | -1.69E-46 |
| MS.gene049378 | MS.gene37952 | 0.809104 | 2.23E-50 | -1.69E-46 |
| MS.gene049389 | MS.gene37952 | 0.80832 | 3.28E-50 | -1.69E-46 |
| MS.gene049491 | MS.gene37952 | 0.821664 | 3.58E-53 | -1.69E-46 |
| MS.gene049758 | MS.gene37952 | 0.886921 | 2.24E-72 | -1.69E-46 |
| MS.gene049910 | MS.gene37952 | 0.853978 | 1.67E-61 | -1.69E-46 |
| MS.gene049915 | MS.gene37952 | 0.814956 | 1.18E-51 | -1.69E-46 |
| MS.gene050128 | MS.gene37952 | 0.846763 | 1.77E-59 | -1.69E-46 |
| MS.gene050226 | MS.gene37952 | 0.810385 | 1.18E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene37952 | 0.810547 | 1.09E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene37952 | 0.811165 | 8.02E-51 | -1.69E-46 |
| MS.gene050301 | MS.gene37952 | 0.880914 | 3.71E-70 | -1.69E-46 |
| MS.gene050397 | MS.gene37952 | 0.855172 | 7.54E-62 | -1.69E-46 |
| MS.gene050398 | MS.gene37952 | 0.80448 | 2.12E-49 | -1.69E-46 |
| MS.gene050404 | MS.gene37952 | 0.81126 | 7.65E-51 | -1.69E-46 |
| MS.gene050406 | MS.gene37952 | 0.830724 | 2.50E-55 | -1.69E-46 |
| MS.gene050426 | MS.gene37952 | -0.830462 | 2.90E-55 | -1.69E-46 |
| MS.gene050464 | MS.gene37952 | 0.858867 | 6.13E-63 | -1.69E-46 |
| MS.gene050529 | MS.gene37952 | 0.857013 | 2.18E-62 | -1.69E-46 |
| MS.gene050558 | MS.gene37952 | 0.807139 | 5.85E-50 | -1.69E-46 |
| MS.gene050623 | MS.gene37952 | -0.844822 | 5.96E-59 | -1.69E-46 |
| MS.gene050642 | MS.gene37952 | 0.881926 | 1.60E-70 | -1.69E-46 |
| MS.gene050650 | MS.gene37952 | 0.819591 | 1.07E-52 | -1.69E-46 |
| MS.gene050670 | MS.gene37952 | 0.848358 | 6.45E-60 | -1.69E-46 |
| MS.gene050971 | MS.gene37952 | 0.811173 | 7.99E-51 | -1.69E-46 |
| MS.gene05101 | MS.gene37952 | 0.825968 | 3.51E-54 | -1.69E-46 |
| MS.gene051471 | MS.gene37952 | 0.828761 | 7.51E-55 | -1.69E-46 |
| MS.gene051582 | MS.gene37952 | 0.832483 | 9.21E-56 | -1.69E-46 |
| MS.gene051662 | MS.gene37952 | 0.812155 | 4.89E-51 | -1.69E-46 |
| MS.gene051663 | MS.gene37952 | 0.824028 | 1.01E-53 | -1.69E-46 |
| MS.gene051860 | MS.gene37952 | 0.861043 | 1.35E-63 | -1.69E-46 |
| MS.gene051983 | MS.gene37952 | 0.885456 | 8.00E-72 | -1.69E-46 |
| MS.gene052011 | MS.gene37952 | -0.835314 | 1.80E-56 | -1.69E-46 |
| MS.gene052077 | MS.gene37952 | 0.87137 | 7.20E-67 | -1.69E-46 |
| MS.gene052078 | MS.gene37952 | 0.857632 | 1.43E-62 | -1.69E-46 |
| MS.gene052178 | MS.gene37952 | 0.855568 | 5.78E-62 | -1.69E-46 |
| MS.gene052188 | MS.gene37952 | 0.878726 | 2.22E-69 | -1.69E-46 |
| MS.gene052253 | MS.gene37952 | 0.856258 | 3.63E-62 | -1.69E-46 |
| MS.gene052309 | MS.gene37952 | -0.822826 | 1.92E-53 | -1.69E-46 |
| MS.gene052310 | MS.gene37952 | 0.848513 | 5.84E-60 | -1.69E-46 |
| MS.gene052340 | MS.gene37952 | 0.884592 | 1.68E-71 | -1.69E-46 |
| MS.gene052485 | MS.gene37952 | 0.827463 | 1.54E-54 | -1.69E-46 |
| MS.gene052600 | MS.gene37952 | 0.802585 | 5.23E-49 | -1.69E-46 |
| MS.gene05316 | MS.gene37952 | 0.825218 | 5.29E-54 | -1.69E-46 |
| MS.gene053209 | MS.gene37952 | 0.821747 | 3.42E-53 | -1.69E-46 |
| MS.gene053557 | MS.gene37952 | 0.864477 | 1.18E-64 | -1.69E-46 |
| MS.gene053559 | MS.gene37952 | 0.805161 | 1.53E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene37952 | 0.911532 | 5.47E-83 | -1.69E-46 |
| MS.gene053907 | MS.gene37952 | 0.851923 | 6.47E-61 | -1.69E-46 |
| MS.gene053928 | MS.gene37952 | 0.831195 | 1.92E-55 | -1.69E-46 |
| MS.gene054011 | MS.gene37952 | 0.810609 | 1.06E-50 | -1.69E-46 |
| MS.gene054123 | MS.gene37952 | 0.827797 | 1.28E-54 | -1.69E-46 |
| MS.gene054183 | MS.gene37952 | 0.832121 | 1.13E-55 | -1.69E-46 |
| MS.gene054349 | MS.gene37952 | 0.839698 | 1.36E-57 | -1.69E-46 |
| MS.gene054473 | MS.gene37952 | 0.85186 | 6.74E-61 | -1.69E-46 |
| MS.gene054930 | MS.gene37952 | 0.857562 | 1.50E-62 | -1.69E-46 |
| MS.gene054942 | MS.gene37952 | 0.822671 | 2.09E-53 | -1.69E-46 |
| MS.gene054983 | MS.gene37952 | 0.86419 | 1.45E-64 | -1.69E-46 |
| MS.gene055116 | MS.gene37952 | 0.842328 | 2.77E-58 | -1.69E-46 |
| MS.gene055277 | MS.gene37952 | 0.851619 | 7.89E-61 | -1.69E-46 |
| MS.gene055390 | MS.gene37952 | 0.821985 | 3.02E-53 | -1.69E-46 |
| MS.gene055422 | MS.gene37952 | 0.886953 | 2.18E-72 | -1.69E-46 |
| MS.gene055428 | MS.gene37952 | 0.862727 | 4.13E-64 | -1.69E-46 |
| MS.gene05553 | MS.gene37952 | 0.860819 | 1.58E-63 | -1.69E-46 |
| MS.gene055604 | MS.gene37952 | 0.831309 | 1.80E-55 | -1.69E-46 |
| MS.gene055712 | MS.gene37952 | 0.840025 | 1.11E-57 | -1.69E-46 |
| MS.gene055859 | MS.gene37952 | 0.804309 | 2.30E-49 | -1.69E-46 |
| MS.gene056191 | MS.gene37952 | 0.818323 | 2.08E-52 | -1.69E-46 |
| MS.gene056314 | MS.gene37952 | 0.800336 | 1.51E-48 | -1.69E-46 |
| MS.gene056407 | MS.gene37952 | 0.831595 | 1.53E-55 | -1.69E-46 |
| MS.gene056609 | MS.gene37952 | 0.803845 | 2.87E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene37952 | 0.866192 | 3.41E-65 | -1.69E-46 |
| MS.gene056659 | MS.gene37952 | 0.862279 | 5.67E-64 | -1.69E-46 |
| MS.gene056723 | MS.gene37952 | 0.818116 | 2.32E-52 | -1.69E-46 |
| MS.gene056899 | MS.gene37952 | -0.837139 | 6.20E-57 | -1.69E-46 |
| MS.gene056979 | MS.gene37952 | 0.887441 | 1.42E-72 | -1.69E-46 |
| MS.gene05702 | MS.gene37952 | 0.859681 | 3.50E-63 | -1.69E-46 |
| MS.gene057121 | MS.gene37952 | 0.847682 | 9.91E-60 | -1.69E-46 |
| MS.gene057245 | MS.gene37952 | 0.830553 | 2.75E-55 | -1.69E-46 |
| MS.gene057439 | MS.gene37952 | 0.840978 | 6.28E-58 | -1.69E-46 |
| MS.gene057448 | MS.gene37952 | 0.82753 | 1.49E-54 | -1.69E-46 |
| MS.gene057449 | MS.gene37952 | 0.821062 | 4.93E-53 | -1.69E-46 |
| MS.gene057450 | MS.gene37952 | 0.834124 | 3.59E-56 | -1.69E-46 |
| MS.gene057469 | MS.gene37952 | 0.802222 | 6.22E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene37952 | 0.8113 | 7.50E-51 | -1.69E-46 |
| MS.gene057754 | MS.gene37952 | 0.808396 | 3.16E-50 | -1.69E-46 |
| MS.gene057823 | MS.gene37952 | 0.805641 | 1.21E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene37952 | 0.896236 | 4.48E-76 | -1.69E-46 |
| MS.gene058082 | MS.gene37952 | 0.837453 | 5.15E-57 | -1.69E-46 |
| MS.gene05811 | MS.gene37952 | 0.883428 | 4.52E-71 | -1.69E-46 |
| MS.gene058142 | MS.gene37952 | 0.887222 | 1.72E-72 | -1.69E-46 |
| MS.gene058233 | MS.gene37952 | 0.818992 | 1.47E-52 | -1.69E-46 |
| MS.gene058276 | MS.gene37952 | 0.848306 | 6.66E-60 | -1.69E-46 |
| MS.gene058293 | MS.gene37952 | 0.807818 | 4.20E-50 | -1.69E-46 |
| MS.gene058435 | MS.gene37952 | 0.801282 | 9.69E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene37952 | 0.845649 | 3.56E-59 | -1.69E-46 |
| MS.gene058710 | MS.gene37952 | 0.855796 | 4.96E-62 | -1.69E-46 |
| MS.gene058749 | MS.gene37952 | 0.848337 | 6.53E-60 | -1.69E-46 |
| MS.gene058787 | MS.gene37952 | 0.835289 | 1.83E-56 | -1.69E-46 |
| MS.gene058833 | MS.gene37952 | 0.800372 | 1.49E-48 | -1.69E-46 |
| MS.gene058845 | MS.gene37952 | 0.856371 | 3.37E-62 | -1.69E-46 |
| MS.gene05886 | MS.gene37952 | 0.835104 | 2.04E-56 | -1.69E-46 |
| MS.gene058928 | MS.gene37952 | 0.825402 | 4.78E-54 | -1.69E-46 |
| MS.gene05907 | MS.gene37952 | 0.884005 | 2.77E-71 | -1.69E-46 |
| MS.gene059247 | MS.gene37952 | 0.811548 | 6.63E-51 | -1.69E-46 |
| MS.gene059285 | MS.gene37952 | 0.863571 | 2.26E-64 | -1.69E-46 |
| MS.gene059294 | MS.gene37952 | 0.82214 | 2.78E-53 | -1.69E-46 |
| MS.gene059420 | MS.gene37952 | 0.827031 | 1.96E-54 | -1.69E-46 |
| MS.gene059518 | MS.gene37952 | 0.874059 | 9.08E-68 | -1.69E-46 |
| MS.gene059597 | MS.gene37952 | 0.862234 | 5.86E-64 | -1.69E-46 |
| MS.gene05959 | MS.gene37952 | 0.825516 | 4.49E-54 | -1.69E-46 |
| MS.gene059676 | MS.gene37952 | 0.845745 | 3.35E-59 | -1.69E-46 |
| MS.gene059688 | MS.gene37952 | 0.834593 | 2.74E-56 | -1.69E-46 |
| MS.gene059692 | MS.gene37952 | 0.85011 | 2.10E-60 | -1.69E-46 |
| MS.gene059695 | MS.gene37952 | 0.841252 | 5.32E-58 | -1.69E-46 |
| MS.gene059708 | MS.gene37952 | 0.820994 | 5.11E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene37952 | 0.911035 | 9.59E-83 | -1.69E-46 |
| MS.gene059894 | MS.gene37952 | 0.89286 | 1.07E-74 | -1.69E-46 |
| MS.gene059899 | MS.gene37952 | 0.850451 | 1.68E-60 | -1.69E-46 |
| MS.gene060247 | MS.gene37952 | 0.812552 | 4.00E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene37952 | 0.838867 | 2.23E-57 | -1.69E-46 |
| MS.gene060403 | MS.gene37952 | 0.801186 | 1.01E-48 | -1.69E-46 |
| MS.gene060407 | MS.gene37952 | 0.852372 | 4.82E-61 | -1.69E-46 |
| MS.gene060476 | MS.gene37952 | 0.804567 | 2.03E-49 | -1.69E-46 |
| MS.gene060495 | MS.gene37952 | 0.808952 | 2.41E-50 | -1.69E-46 |
| MS.gene06090 | MS.gene37952 | 0.80517 | 1.52E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene37952 | 0.905497 | 4.03E-80 | -1.69E-46 |
| MS.gene061051 | MS.gene37952 | -0.803376 | 3.59E-49 | -1.69E-46 |
| MS.gene061154 | MS.gene37952 | 0.808582 | 2.89E-50 | -1.69E-46 |
| MS.gene061159 | MS.gene37952 | 0.829159 | 6.01E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37952.t1 | MTR_5g095770 | 100.000 | 73 | 0 | 0 | 1 | 73 | 143 | 215 | 5.77e-46 | 145 |
| MS.gene37952.t1 | MTR_7g080300 | 47.945 | 73 | 37 | 1 | 1 | 72 | 137 | 209 | 2.33e-17 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37952.t1 | AT5G55710 | 80.822 | 73 | 14 | 0 | 1 | 73 | 137 | 209 | 3.01e-35 | 118 |
| MS.gene37952.t1 | AT2G47840 | 48.649 | 74 | 36 | 1 | 1 | 72 | 135 | 208 | 8.18e-16 | 68.6 |
Find 13 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGCTCTTCTTCTTGCATTT+TGG | 0.091593 | 5.1:+76491984 | MS.gene37952:CDS |
| TTGTTGGTGTGTTTGATTTA+TGG | 0.141836 | 5.1:+76491964 | MS.gene37952:CDS |
| ATTGGATTTGTTGATGAGTT+TGG | 0.285770 | 5.1:+76491921 | MS.gene37952:CDS |
| TTTAATCCAAGAGGTGGGTT+TGG | 0.383345 | 5.1:+76491898 | MS.gene37952:CDS |
| AACCCTCTTTCCAAAAGATC+AGG | 0.429515 | 5.1:-76491877 | None:intergenic |
| TCCAATCCAAACCCACCTCT+TGG | 0.441020 | 5.1:-76491904 | None:intergenic |
| TCCAAGAGGTGGGTTTGGAT+TGG | 0.441807 | 5.1:+76491903 | MS.gene37952:CDS |
| TCAGCAGCATCAGCAACAAT+AGG | 0.464175 | 5.1:-76492024 | None:intergenic |
| TTGTTGCTGATGCTGCTGAT+AGG | 0.467577 | 5.1:+76492028 | MS.gene37952:CDS |
| AGAGGGTTTAATCCAAGAGG+TGG | 0.570836 | 5.1:+76491892 | MS.gene37952:CDS |
| GAGGGTTTAATCCAAGAGGT+GGG | 0.602525 | 5.1:+76491893 | MS.gene37952:CDS |
| TCAGCAACAATAGGCAACCT+AGG | 0.619404 | 5.1:-76492015 | None:intergenic |
| GAAAGAGGGTTTAATCCAAG+AGG | 0.654426 | 5.1:+76491889 | MS.gene37952:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTGTTGGTGTGTTTGATTTA+TGG | + | chr5.1:76491964-76491983 | MS.gene37952:CDS | 30.0% |
| !! | ATTGGATTTGTTGATGAGTT+TGG | + | chr5.1:76491921-76491940 | MS.gene37952:CDS | 30.0% |
| !! | GTTGATTTTTCCTGATCTTT+TGG | + | chr5.1:76491867-76491886 | MS.gene37952:CDS | 30.0% |
| !! | CACTGTGTTTTTGTTCTTGT+TGG | + | chr5.1:76491948-76491967 | MS.gene37952:CDS | 35.0% |
| !! | TTCCTGATCTTTTGGAAAGA+GGG | + | chr5.1:76491875-76491894 | MS.gene37952:CDS | 35.0% |
| !! | TTTCCTGATCTTTTGGAAAG+AGG | + | chr5.1:76491874-76491893 | MS.gene37952:CDS | 35.0% |
| AACCCTCTTTCCAAAAGATC+AGG | - | chr5.1:76491880-76491899 | None:intergenic | 40.0% | |
| GAAAGAGGGTTTAATCCAAG+AGG | + | chr5.1:76491889-76491908 | MS.gene37952:CDS | 40.0% | |
| ! | GGCTCTTCTTCTTGCATTTT+GGG | + | chr5.1:76491985-76492004 | MS.gene37952:CDS | 40.0% |
| ! | TGGCTCTTCTTCTTGCATTT+TGG | + | chr5.1:76491984-76492003 | MS.gene37952:CDS | 40.0% |
| ! | TTTAATCCAAGAGGTGGGTT+TGG | + | chr5.1:76491898-76491917 | MS.gene37952:CDS | 40.0% |
| AGAGGGTTTAATCCAAGAGG+TGG | + | chr5.1:76491892-76491911 | MS.gene37952:CDS | 45.0% | |
| GAGGGTTTAATCCAAGAGGT+GGG | + | chr5.1:76491893-76491912 | MS.gene37952:CDS | 45.0% | |
| TCAGCAACAATAGGCAACCT+AGG | - | chr5.1:76492018-76492037 | None:intergenic | 45.0% | |
| TCAGCAGCATCAGCAACAAT+AGG | - | chr5.1:76492027-76492046 | None:intergenic | 45.0% | |
| TTGTTGCTGATGCTGCTGAT+AGG | + | chr5.1:76492028-76492047 | MS.gene37952:CDS | 45.0% | |
| TCCAATCCAAACCCACCTCT+TGG | - | chr5.1:76491907-76491926 | None:intergenic | 50.0% | |
| ! | TCCAAGAGGTGGGTTTGGAT+TGG | + | chr5.1:76491903-76491922 | MS.gene37952:CDS | 50.0% |
| !! | GCATTTTGGGTCAGCTACCT+AGG | + | chr5.1:76491998-76492017 | MS.gene37952:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 76491841 | 76492062 | 76491841 | ID=MS.gene37952 |
| chr5.1 | mRNA | 76491841 | 76492062 | 76491841 | ID=MS.gene37952.t1;Parent=MS.gene37952 |
| chr5.1 | exon | 76491841 | 76492062 | 76491841 | ID=MS.gene37952.t1.exon1;Parent=MS.gene37952.t1 |
| chr5.1 | CDS | 76491841 | 76492062 | 76491841 | ID=cds.MS.gene37952.t1;Parent=MS.gene37952.t1 |
| Gene Sequence |
| Protein sequence |
