AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38339


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38339.t1 MTR_7g045270 78.899 109 20 1 1 106 1 109 5.22e-58 179
MS.gene38339.t1 MTR_4g036465 31.604 212 114 5 46 229 66 274 2.36e-24 97.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38339.t1 AT4G25170 27.679 224 118 5 47 226 118 341 1.39e-22 94.4
MS.gene38339.t1 AT4G25170 25.941 239 118 5 47 226 118 356 1.97e-20 89.0
MS.gene38339.t1 AT5G61490 30.412 194 113 5 47 226 70 255 2.17e-16 76.3

Find 37 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTGGAGTTGCCTGATTC+AGG 0.234726 7.3:-57414422 MS.gene38339:intron
GCAAGCTGATCCTGCTGTTC+AGG 0.270311 7.3:-57414910 MS.gene38339:intron
TAGCCATGATTACAATTAAA+AGG 0.291956 7.3:+57413844 None:intergenic
TGTGTTGCAGCAAAGGCTTC+TGG 0.299635 7.3:-57414438 MS.gene38339:CDS
TTATCTTCTTGTTGCTGTAA+TGG 0.335551 7.3:-57415778 MS.gene38339:CDS
ATCATCTGATGAAGCCATTT+GGG 0.369589 7.3:-57414477 MS.gene38339:CDS
TGCAATCTCAAGGATATAAT+AGG 0.380708 7.3:-57414956 MS.gene38339:CDS
TATCATCTGATGAAGCCATT+TGG 0.380798 7.3:-57414478 MS.gene38339:CDS
ACTCTTATGATTATGATTCA+AGG 0.400681 7.3:-57414983 MS.gene38339:CDS
GTAAAATACAGACCTGAATC+AGG 0.413501 7.3:+57414410 None:intergenic
TCAGCGTAGAAATCTTAAAC+TGG 0.444519 7.3:-57414025 MS.gene38339:CDS
TGACACTGTTCCATCTCAAA+TGG 0.457616 7.3:-57415698 MS.gene38339:CDS
CATTATCAATCCCAGGGGAC+TGG 0.459985 7.3:+57413931 None:intergenic
CCTGGGATTGATAATGCAAC+AGG 0.460382 7.3:-57413924 MS.gene38339:CDS
AATGATTTATTCCAGTCCCC+TGG 0.471686 7.3:-57413942 MS.gene38339:CDS
TATCATGAAAGGAAAAGTCT+TGG 0.473359 7.3:-57413994 MS.gene38339:CDS
ATTACAGCAACAAGAAGATA+AGG 0.483282 7.3:+57415780 None:intergenic
AAGAGAGAAACCTGAACAGC+AGG 0.493868 7.3:+57414900 None:intergenic
TAAGAAATGTGTTGCAGCAA+AGG 0.518969 7.3:-57414445 MS.gene38339:CDS
TCCTCAACTTCCATTTGAGA+TGG 0.521148 7.3:+57415688 None:intergenic
GATGGAACAGTGTCAAATAC+AGG 0.521605 7.3:+57415706 None:intergenic
TCTTATGACAGCATCCCAAA+TGG 0.524517 7.3:+57414463 None:intergenic
AGAAAACAAGAGATAACATC+AGG 0.536095 7.3:-57415745 MS.gene38339:CDS
ATGATTTATTCCAGTCCCCT+GGG 0.537176 7.3:-57413941 MS.gene38339:CDS
CCTGTTGCATTATCAATCCC+AGG 0.540379 7.3:+57413924 None:intergenic
TCAAGGATTGTGCAATCTCA+AGG 0.559981 7.3:-57414966 MS.gene38339:CDS
CATCATTCATCAATGGGAGG+TGG 0.560723 7.3:-57415853 None:intergenic
TACAATTAAAAGGATAACAA+TGG 0.569576 7.3:+57413854 None:intergenic
TCCATCTCAAATGGAAGTTG+AGG 0.577676 7.3:-57415689 MS.gene38339:CDS
AACAGGTGATACATCACAGT+TGG 0.603158 7.3:-57413907 MS.gene38339:CDS
CTGTTGCATTATCAATCCCA+GGG 0.603976 7.3:+57413925 None:intergenic
GATCTGTTGATCTATGATTG+TGG 0.615202 7.3:+57415010 None:intergenic
TCTCTTTGTTCTGAACTCTG+AGG 0.618675 7.3:+57414050 None:intergenic
TTATTGGCGTTACTCAACTG+AGG 0.661229 7.3:+57415805 None:intergenic
AAACAAGAGATAACATCAGG+TGG 0.661795 7.3:-57415742 MS.gene38339:CDS
AATCATCATTCATCAATGGG+AGG 0.669157 7.3:-57415856 None:intergenic
TGTTGCATTATCAATCCCAG+GGG 0.757453 7.3:+57413926 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAAGAAATATAATTAATTT+AGG - chr7.3:57414185-57414204 MS.gene38339:intron 10.0%
!! TCGTAAAATGAAATATATAA+TGG - chr7.3:57415517-57415536 MS.gene38339:intron 15.0%
!! AATTTAGTGTAATTATGTGT+TGG - chr7.3:57415435-57415454 MS.gene38339:intron 20.0%
!! GAACTTTAAATTCAAGAAAA+TGG - chr7.3:57414281-57414300 MS.gene38339:intron 20.0%
!! TACAATTAAAAGGATAACAA+TGG + chr7.3:57415811-57415830 None:intergenic 20.0%
!!! GAAAAATAAAAGGAACTTTT+GGG - chr7.3:57414259-57414278 MS.gene38339:intron 20.0%
!!! TATCCTTTTAATTGTAATCA+TGG - chr7.3:57415815-57415834 MS.gene38339:CDS 20.0%
!!! TGATTTTCTCAAATTATTAG+CGG + chr7.3:57415412-57415431 None:intergenic 20.0%
!!! TGGATATTTTATATCATGAA+AGG - chr7.3:57415657-57415676 MS.gene38339:intron 20.0%
!!! TTAGATGAAATTGTTTGTTT+TGG - chr7.3:57414114-57414133 MS.gene38339:intron 20.0%
!!! TTCTTAGTGAATATTTCTTT+TGG + chr7.3:57415331-57415350 None:intergenic 20.0%
! AAAGAGTAAGAAGTAACATA+AGG + chr7.3:57414057-57414076 None:intergenic 25.0%
! AAATATTCACTAAGAAGAGA+AGG - chr7.3:57415335-57415354 MS.gene38339:intron 25.0%
! AAGAGTAAGAAGTAACATAA+GGG + chr7.3:57414056-57414075 None:intergenic 25.0%
! ACTCTTATGATTATGATTCA+AGG - chr7.3:57414679-57414698 MS.gene38339:intron 25.0%
! AGAAGGAATGATATATAACA+CGG - chr7.3:57415352-57415371 MS.gene38339:intron 25.0%
! AGTTTCAAAACACAATCAAT+TGG - chr7.3:57414824-57414843 MS.gene38339:intron 25.0%
! CTTTATATTGCTACTAAATG+CGG - chr7.3:57414385-57414404 MS.gene38339:intron 25.0%
! TAGCCATGATTACAATTAAA+AGG + chr7.3:57415821-57415840 None:intergenic 25.0%
!! ATATTTCATTTTACGAAGCA+TGG + chr7.3:57415513-57415532 None:intergenic 25.0%
!! CGAAAAATAAAAGGAACTTT+TGG - chr7.3:57414258-57414277 MS.gene38339:intron 25.0%
!! CTTTTTACAAGTTACTACAA+AGG + chr7.3:57414534-57414553 None:intergenic 25.0%
!! TTAGTGAATATTTCTTTTGG+TGG + chr7.3:57415328-57415347 None:intergenic 25.0%
!!! AAAAGTTTTTAAGAATCGTG+TGG - chr7.3:57414549-57414568 MS.gene38339:intron 25.0%
!!! ACAAGAAGATAAGGTTTTAT+TGG + chr7.3:57413876-57413895 None:intergenic 25.0%
!!! ACTCTTTTGAGATAAATTAG+AGG - chr7.3:57414070-57414089 MS.gene38339:intron 25.0%
!!! TATTTTACTAACTTGTGGAA+TGG - chr7.3:57415267-57415286 MS.gene38339:intron 25.0%
!!! TTGATTGTGTTTTGAAACTA+TGG + chr7.3:57414823-57414842 None:intergenic 25.0%
AGAAAACAAGAGATAACATC+AGG - chr7.3:57413917-57413936 MS.gene38339:CDS 30.0%
AGATAAATTAGAGGTCTTCT+TGG - chr7.3:57414079-57414098 MS.gene38339:intron 30.0%
ATTACAGCAACAAGAAGATA+AGG + chr7.3:57413885-57413904 None:intergenic 30.0%
CTACTAAATGCGGAAATAAA+CGG - chr7.3:57414395-57414414 MS.gene38339:intron 30.0%
GATAAATTAGAGGTCTTCTT+GGG - chr7.3:57414080-57414099 MS.gene38339:intron 30.0%
TAAGAAATGTTACGACCATT+AGG + chr7.3:57414485-57414504 None:intergenic 30.0%
TATCATGAAAGGAAAAGTCT+TGG - chr7.3:57415668-57415687 MS.gene38339:CDS 30.0%
TCTGACACAACAATTTGAAA+AGG + chr7.3:57414613-57414632 None:intergenic 30.0%
TGCAATCTCAAGGATATAAT+AGG - chr7.3:57414706-57414725 MS.gene38339:intron 30.0%
TGGAAGAAAACAATGTTAGA+TGG + chr7.3:57415573-57415592 None:intergenic 30.0%
TTATCTTCTTGTTGCTGTAA+TGG - chr7.3:57413884-57413903 MS.gene38339:CDS 30.0%
TTGTTGCTGCGAAAAATAAA+AGG - chr7.3:57414249-57414268 MS.gene38339:intron 30.0%
! ACTCAATACAAAAGGTTTTG+AGG + chr7.3:57414462-57414481 None:intergenic 30.0%
! ATTTTTCGCAGCAACAAATA+TGG + chr7.3:57414246-57414265 None:intergenic 30.0%
! GGAAAGAAAGCTGAATATTT+TGG + chr7.3:57414513-57414532 None:intergenic 30.0%
! GTCTGTATTTTACTAACTTG+TGG - chr7.3:57415262-57415281 MS.gene38339:intron 30.0%
!!! AACTCGTTGCTTTAAGATTT+TGG - chr7.3:57414923-57414942 MS.gene38339:CDS 30.0%
AAACAAGAGATAACATCAGG+TGG - chr7.3:57413920-57413939 MS.gene38339:CDS 35.0%
ATCATCTGATGAAGCCATTT+GGG - chr7.3:57415185-57415204 MS.gene38339:intron 35.0%
CACAGTTGGATGAAAAAGTT+AGG - chr7.3:57415769-57415788 MS.gene38339:CDS 35.0%
CCATTAGGACTCAATACAAA+AGG + chr7.3:57414470-57414489 None:intergenic 35.0%
GATCTGTTGATCTATGATTG+TGG + chr7.3:57414655-57414674 None:intergenic 35.0%
GGTTAATGTAGTCGAAATTG+CGG - chr7.3:57414416-57414435 MS.gene38339:intron 35.0%
GTAAAATACAGACCTGAATC+AGG + chr7.3:57415255-57415274 None:intergenic 35.0%
GTGACTTATATGTCGTACAT+CGG - chr7.3:57414857-57414876 MS.gene38339:intron 35.0%
TAAGAAATGTGTTGCAGCAA+AGG - chr7.3:57415217-57415236 MS.gene38339:intron 35.0%
TATCATCTGATGAAGCCATT+TGG - chr7.3:57415184-57415203 MS.gene38339:intron 35.0%
TCAGCGTAGAAATCTTAAAC+TGG - chr7.3:57415637-57415656 MS.gene38339:intron 35.0%
TCTCAAATTATTAGCGGTGT+TGG + chr7.3:57415406-57415425 None:intergenic 35.0%
TTATATGTCGTACATCGGAT+AGG - chr7.3:57414862-57414881 MS.gene38339:intron 35.0%
! CCTTTTGTATTGAGTCCTAA+TGG - chr7.3:57414467-57414486 MS.gene38339:CDS 35.0%
! GTGTAATTATGTGTTGGTGT+CGG - chr7.3:57415441-57415460 MS.gene38339:intron 35.0%
AACAGGTGATACATCACAGT+TGG - chr7.3:57415755-57415774 MS.gene38339:CDS 40.0%
AATGATTTATTCCAGTCCCC+TGG - chr7.3:57415720-57415739 MS.gene38339:CDS 40.0%
ATGATTTATTCCAGTCCCCT+GGG - chr7.3:57415721-57415740 MS.gene38339:CDS 40.0%
CTGTTGCATTATCAATCCCA+GGG + chr7.3:57415740-57415759 None:intergenic 40.0%
CTTAATCCTTAAAGCCGTGT+CGG + chr7.3:57415033-57415052 None:intergenic 40.0%
GATGGAACAGTGTCAAATAC+AGG + chr7.3:57413959-57413978 None:intergenic 40.0%
TACATCGGATAGGAAAGTAG+AGG - chr7.3:57414872-57414891 MS.gene38339:intron 40.0%
TCAAGGATTGTGCAATCTCA+AGG - chr7.3:57414696-57414715 MS.gene38339:intron 40.0%
TCCATCTCAAATGGAAGTTG+AGG - chr7.3:57413973-57413992 MS.gene38339:CDS 40.0%
TCCTCAACTTCCATTTGAGA+TGG + chr7.3:57413977-57413996 None:intergenic 40.0%
TCTTATGACAGCATCCCAAA+TGG + chr7.3:57415202-57415221 None:intergenic 40.0%
TGACACTGTTCCATCTCAAA+TGG - chr7.3:57413964-57413983 MS.gene38339:CDS 40.0%
TGTTGCATTATCAATCCCAG+GGG + chr7.3:57415739-57415758 None:intergenic 40.0%
TTAATCCTTAAAGCCGTGTC+GGG + chr7.3:57415032-57415051 None:intergenic 40.0%
TTATTGGCGTTACTCAACTG+AGG + chr7.3:57413860-57413879 None:intergenic 40.0%
TTGCGGTTATGTTGTCATTG+CGG - chr7.3:57414433-57414452 MS.gene38339:CDS 40.0%
! GATTAAGCGTGTCTTGTTGT+GGG + chr7.3:57415480-57415499 None:intergenic 40.0%
! GTCTTTTACCTTTAGCTGGA+TGG + chr7.3:57415593-57415612 None:intergenic 40.0%
! GTTTTCTTCCATCCAGCTAA+AGG - chr7.3:57415582-57415601 MS.gene38339:intron 40.0%
! TCTCTTTGTTCTGAACTCTG+AGG + chr7.3:57415615-57415634 None:intergenic 40.0%
! TGAGGTCTTTTACCTTTAGC+TGG + chr7.3:57415597-57415616 None:intergenic 40.0%
! TGTGCTGATGAAATGTGTCT+CGG - chr7.3:57415131-57415150 MS.gene38339:intron 40.0%
AACAAGACACGCTTAATCCG+AGG - chr7.3:57415482-57415501 MS.gene38339:intron 45.0%
AAGAGAGAAACCTGAACAGC+AGG + chr7.3:57414765-57414784 None:intergenic 45.0%
CCTGTTGCATTATCAATCCC+AGG + chr7.3:57415741-57415760 None:intergenic 45.0%
TAGAGTCTCTCTCACTTGTG+TGG - chr7.3:57414955-57414974 MS.gene38339:CDS 45.0%
! GGATTAAGCGTGTCTTGTTG+TGG + chr7.3:57415481-57415500 None:intergenic 45.0%
!! CCTGGGATTGATAATGCAAC+AGG - chr7.3:57415738-57415757 MS.gene38339:CDS 45.0%
CATTATCAATCCCAGGGGAC+TGG + chr7.3:57415734-57415753 None:intergenic 50.0%
TACGAAGCATGGACACTCCT+CGG + chr7.3:57415502-57415521 None:intergenic 50.0%
TCTGCCAGTCTGCTTTGATG+CGG - chr7.3:57414982-57415001 MS.gene38339:CDS 50.0%
TGTGTTGCAGCAAAGGCTTC+TGG - chr7.3:57415224-57415243 MS.gene38339:intron 50.0%
! GTCTTGTTGTGGGACATCTG+CGG + chr7.3:57415470-57415489 None:intergenic 50.0%
!! CTTCTGGAGTTGCCTGATTC+AGG - chr7.3:57415240-57415259 MS.gene38339:intron 50.0%
AAAGCCGTGTCGGGTCATGA+GGG + chr7.3:57415023-57415042 None:intergenic 55.0%
CATGACCCGACACGGCTTTA+AGG - chr7.3:57415024-57415043 MS.gene38339:CDS 55.0%
CTGCCAGTCTGCTTTGATGC+GGG - chr7.3:57414983-57415002 MS.gene38339:CDS 55.0%
GCAAGCTGATCCTGCTGTTC+AGG - chr7.3:57414752-57414771 MS.gene38339:intron 55.0%
TAAAGCCGTGTCGGGTCATG+AGG + chr7.3:57415024-57415043 None:intergenic 55.0%
AAGCCGTGTCGGGTCATGAG+GGG + chr7.3:57415022-57415041 None:intergenic 60.0%
ACTCCCCTCATGACCCGACA+CGG - chr7.3:57415016-57415035 MS.gene38339:CDS 60.0%
CCACCCGCATCAAAGCAGAC+TGG + chr7.3:57414989-57415008 None:intergenic 60.0%
CCAGTCTGCTTTGATGCGGG+TGG - chr7.3:57414986-57415005 MS.gene38339:CDS 60.0%
CGGGTCATGAGGGGAGTCCA+GGG + chr7.3:57415013-57415032 None:intergenic 65.0%
GCTTTGATGCGGGTGGTCCC+TGG - chr7.3:57414993-57415012 MS.gene38339:CDS 65.0%
TCGGGTCATGAGGGGAGTCC+AGG + chr7.3:57415014-57415033 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 57413821 57415863 57413821 ID=MS.gene38339
chr7.3 mRNA 57413821 57415863 57413821 ID=MS.gene38339.t1;Parent=MS.gene38339
chr7.3 exon 57415667 57415863 57415667 ID=MS.gene38339.t1.exon1;Parent=MS.gene38339.t1
chr7.3 CDS 57415667 57415863 57415667 ID=cds.MS.gene38339.t1;Parent=MS.gene38339.t1
chr7.3 exon 57414911 57415052 57414911 ID=MS.gene38339.t1.exon2;Parent=MS.gene38339.t1
chr7.3 CDS 57414911 57415052 57414911 ID=cds.MS.gene38339.t1;Parent=MS.gene38339.t1
chr7.3 exon 57414423 57414516 57414423 ID=MS.gene38339.t1.exon3;Parent=MS.gene38339.t1
chr7.3 CDS 57414423 57414516 57414423 ID=cds.MS.gene38339.t1;Parent=MS.gene38339.t1
chr7.3 exon 57413821 57414086 57413821 ID=MS.gene38339.t1.exon4;Parent=MS.gene38339.t1
chr7.3 CDS 57413821 57414086 57413821 ID=cds.MS.gene38339.t1;Parent=MS.gene38339.t1
Gene Sequence

>MS.gene38339

ATGGGAGGTGGAACTATGCATATTGATCATCTTGTACCTCAGTTGAGTAACGCCAATAAAACCTTATCTTCTTGTTGCTGTAATGGTCAGTTCTGCAGAAAACAAGAGATAACATCAGGTGGTAATCTCTATACTCCTGTATTTGACACTGTTCCATCTCAAATGGAAGTTGAGGATGCAATTTCAGCTCTTCAAGAGTGAGTTTCTTGTTTTGTGAATTAACTAGTTTATTCCCTTATGTTACTTCTTACTCTTTTGAGATAAATTAGAGGTCTTCTTGGGAAGCTTTTTGATTAGATGAAATTGTTTGTTTTGGAATATATATCAAGAAAAGATAATTAATGTAGTGCTGTGATATTGTGCACTAAGAAATATAATTAATTTAGGATTTCTTTAAATTCAAGAAAATAGTATTATAGCTTCCATATTTGTTGCTGCGAAAAATAAAAGGAACTTTTGGGAACTTTAAATTCAAGAAAATGGTAGTATTATAACTTCAACTTTATTAGTGTCATTTTTTTTTTTTGACAATATAGTGATTTTAAAAAATTGCTGTCTGCAATGCTTTATATTGCTACTAAATGCGGAAATAAACGGTTAATGTAGTCGAAATTGCGGTTATGTTGTCATTGCGGAGACCTCAAAACCTTTTGTATTGAGTCCTAATGGTCGTAACATTTCTTATTACACCAAAATATTCAGCTTTCTTTCCTTTGTAGTAACTTGTAAAAAGTTTTTAAGAATCGTGTGGCAAAATTTTATACAAGCAATAATAACAAGTTGAGCATTCCTTTTCAAATTGTTGTGTCAGATTTATGAAAGCAGTATCAACCACAATCATAGATCAACAGATCGCAGACTCTTATGATTATGATTCAAGGATTGTGCAATCTCAAGGATATAATAGGCTTTACAATGCTCTTCAGTTGCTGCAAGCTGATCCTGCTGTTCAGGTTTCTCTCTTGACTTGTATAGTTTCAAAACATTGAAAAGCATTTTCCATAGTTTCAAAACACAATCAATTGGTGTTGCAAGTGTGACTTATATGTCGTACATCGGATAGGAAAGTAGAGGTTGAACACTTTATAAGAAAAATGACACAAACTCGTTGCTTTAAGATTTTGGATTAAAGTGTAGAGTCTCTCTCACTTGTGTGGTTGCTCTGCCAGTCTGCTTTGATGCGGGTGGTCCCTGGACTCCCCTCATGACCCGACACGGCTTTAAGGATTAAGAGACATCATGAAAATTCGAATCTTTTTAACGATATTTTATTTGTTTTTCTGACTTTAGTTGTTGATATTGTTTAATTTTGTGCTGATGAAATGTGTCTCGGATTATAACTTGTAGTGTTTAGTGATTTCACTATCATCTGATGAAGCCATTTGGGATGCTGTCATAAGAAATGTGTTGCAGCAAAGGCTTCTGGAGTTGCCTGATTCAGGTCTGTATTTTACTAACTTGTGGAATGGAATTATGAAAGACGAAATGAAAACTATAACAACGACCACCAAAAGAAATATTCACTAAGAAGAGAAGGAATGATATATAACACGGATACAGACACAGACACAACACTGACATGCCAACACCGCTAATAATTTGAGAAAATCACATAATTTAGTGTAATTATGTGTTGGTGTCGGACACCGCAGATGTCCCACAACAAGACACGCTTAATCCGAGGAGTGTCCATGCTTCGTAAAATGAAATATATAATGGTTTTTCTTTCATAATATCTCACAAGTGTTTCCATCTAACATTGTTTTCTTCCATCCAGCTAAAGGTAAAAGACCTCAGAGTTCAGAACAAAGAGAGATCAGCGTAGAAATCTTAAACTGGATATTTTATATCATGAAAGGAAAAGTCTTGGAACTGATACAAAGTTTTCAGTCACTTATGAATGATTTATTCCAGTCCCCTGGGATTGATAATGCAACAGGTGATACATCACAGTTGGATGAAAAAGTTAGGTCATCTACACTTCTTTCCATTGTTATCCTTTTAATTGTAATCATGGCTAGATATCAAAGTCGTCAAGTCTAA

Protein sequence

>MS.gene38339.t1

MGGGTMHIDHLVPQLSNANKTLSSCCCNGQFCRKQEITSGGNLYTPVFDTVPSQMEVEDAISALQEFMKAVSTTIIDQQIADSYDYDSRIVQSQGYNRLYNALQLLQADPAVQCLVISLSSDEAIWDAVIRNVLQQRLLELPDSAKGKRPQSSEQREISVEILNWIFYIMKGKVLELIQSFQSLMNDLFQSPGIDNATGDTSQLDEKVRSSTLLSIVILLIVIMARYQSRQV