AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54380.t1 MTR_7g006040 98.684 228 3 0 1 228 167 394 1.20e-166 465
MS.gene54380.t1 MTR_8g064720 70.312 192 52 3 1 188 187 377 1.34e-86 262
MS.gene54380.t1 MTR_5g072900 38.824 170 89 5 33 189 219 386 8.90e-24 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54380.t1 AT1G18480 61.638 232 79 5 1 228 166 391 1.23e-91 275
MS.gene54380.t1 AT1G07010 36.471 170 95 4 33 190 204 372 4.45e-21 90.9
MS.gene54380.t1 AT1G07010 36.471 170 95 4 33 190 208 376 4.60e-21 90.9
MS.gene54380.t1 AT1G07010 36.471 170 95 4 33 190 219 387 5.97e-21 90.5

Find 70 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCTAGGGTTGCGGCTTTA+AGG 0.182522 7.4:-93611283 MS.gene54380:CDS
TTGAAAGAACATGTTGATTA+TGG 0.215908 7.4:-93611173 MS.gene54380:CDS
TGAGGTTAGTGATTGGTATA+AGG 0.246230 7.4:-93611132 MS.gene54380:CDS
AAGAGTTTCATGATGGGTTT+AGG 0.248900 7.4:-93611304 MS.gene54380:CDS
TGGTGTTAAGAGGATGATTA+TGG 0.249368 7.4:-93610985 MS.gene54380:CDS
TGGTGGTTTGCCCGAGGTTT+TGG 0.263789 7.4:-93610877 MS.gene54380:CDS
ACGTGGCGTTTCGTGGTGTT+AGG 0.288942 7.4:-93611328 MS.gene54380:CDS
TTATTCATCTGATTATATAA+AGG 0.310568 7.4:+93610816 None:intergenic
TAATGGTGTCTGCGAAAATA+AGG 0.312538 7.4:-93610937 MS.gene54380:CDS
TACACAAAATGTTACTGTTT+TGG 0.322237 7.4:-93611231 MS.gene54380:CDS
TTTATATAATCAGATGAATA+AGG 0.349298 7.4:-93610814 MS.gene54380:CDS
GACAATAAGGCGGCGAAAAC+CGG 0.357356 7.4:+93611096 None:intergenic
GAGGTTAGTGATTGGTATAA+GGG 0.359259 7.4:-93611131 MS.gene54380:CDS
GATTGGTATAAGGGTTTGTT+TGG 0.360244 7.4:-93611122 MS.gene54380:CDS
GGGAAGGTGGAAGAAGGGTT+TGG 0.362537 7.4:-93610777 MS.gene54380:CDS
CGAATGGACCGATTTCAAAA+AGG 0.363226 7.4:-93611259 MS.gene54380:CDS
ATTCAGAAGGAAGGGATTAA+TGG 0.366442 7.4:-93610954 MS.gene54380:CDS
AGAGTTTCATGATGGGTTTA+GGG 0.373309 7.4:-93611303 MS.gene54380:CDS
GCATTCCTTCCCCGACAATA+AGG 0.375199 7.4:+93611083 None:intergenic
TTCATGATGGGTTTAGGGCT+AGG 0.377495 7.4:-93611298 MS.gene54380:CDS
GGGGAAGGAATGCACTTGTT+TGG 0.383176 7.4:-93611073 MS.gene54380:CDS
TATGGGTTGGAGAAGATTAA+TGG 0.389368 7.4:-93611155 MS.gene54380:CDS
TGAAAGAACATGTTGATTAT+GGG 0.411575 7.4:-93611172 MS.gene54380:CDS
ATAAGGGTTTGTTTGGTAAC+CGG 0.411872 7.4:-93611115 MS.gene54380:CDS
TCATGATGGGTTTAGGGCTA+GGG 0.424497 7.4:-93611297 MS.gene54380:CDS
CGACGATTCCTGGTGTTAAG+AGG 0.425093 7.4:-93610995 MS.gene54380:CDS
ATGTAAAGGTTTGGAAGAAA+AGG 0.436989 7.4:-93611375 MS.gene54380:CDS
CGTGGCGTTTCGTGGTGTTA+GGG 0.450367 7.4:-93611327 MS.gene54380:CDS
CGCCGCCTTATTGTCGGGGA+AGG 0.455393 7.4:-93611088 MS.gene54380:CDS
AATGTTCACGTGGCGTTTCG+TGG 0.456234 7.4:-93611335 MS.gene54380:CDS
TCTGCGAAAATAAGGCGATT+AGG 0.459167 7.4:-93610929 MS.gene54380:CDS
GATGTTGGGAAGGTGGAAGA+AGG 0.466879 7.4:-93610783 MS.gene54380:CDS
AATAAGGAAAATGTGGATGT+TGG 0.471510 7.4:-93610798 MS.gene54380:CDS
TGGGTTTAGGGCTAGGGTTG+CGG 0.472943 7.4:-93611291 MS.gene54380:CDS
GGTGTTAAGAGGATGATTAT+GGG 0.476200 7.4:-93610984 MS.gene54380:CDS
AAATGTGGATGTTGGGAAGG+TGG 0.504316 7.4:-93610790 MS.gene54380:CDS
TCAGATGAATAAGGAAAATG+TGG 0.505988 7.4:-93610805 MS.gene54380:CDS
GTTAGGGAAGAGTTTCATGA+TGG 0.506126 7.4:-93611311 MS.gene54380:CDS
ATGTTGGGAAGGTGGAAGAA+GGG 0.512967 7.4:-93610782 MS.gene54380:CDS
AGATTGACCGTTATGGGGTG+AGG 0.524683 7.4:-93610854 MS.gene54380:CDS
GCCTTGACTTCCACTTGCCT+AGG 0.528320 7.4:+93610726 None:intergenic
GCAAGTGGAAGTCAAGGCTT+AGG 0.533119 7.4:-93610721 None:intergenic
ATGTCGAAAGGATGCGGTGG+TGG 0.533822 7.4:-93610894 MS.gene54380:CDS
CGGTCAATCTCCAAAACCTC+GGG 0.534492 7.4:+93610867 None:intergenic
TTAATGGTGAGGTTAGTGAT+TGG 0.539710 7.4:-93611139 MS.gene54380:CDS
ATAAGGAAAATGTGGATGTT+GGG 0.548289 7.4:-93610797 MS.gene54380:CDS
ACCTAGGCAAGTGGAAGTCA+AGG 0.549753 7.4:-93610727 MS.gene54380:CDS
TTAGGGAAGAGTTTCATGAT+GGG 0.558605 7.4:-93611310 MS.gene54380:CDS
ATGCGGTGGTGGTTTGCCCG+AGG 0.563566 7.4:-93610883 MS.gene54380:CDS
TTGTTGCTTAATAATCAAGA+TGG 0.574078 7.4:-93610753 MS.gene54380:CDS
ATCGATGTTGGTATGTCGAA+AGG 0.575221 7.4:-93610906 MS.gene54380:CDS
GTTGGAGAAGATTAATGGTG+AGG 0.579243 7.4:-93611150 MS.gene54380:CDS
GGGCATACGATTCAGAAGGA+AGG 0.584494 7.4:-93610963 MS.gene54380:CDS
TCAATATCCTCACCCCATAA+CGG 0.588832 7.4:+93610847 None:intergenic
ACGGTCAATCTCCAAAACCT+CGG 0.600316 7.4:+93610866 None:intergenic
GGCATACGATTCAGAAGGAA+GGG 0.601131 7.4:-93610962 MS.gene54380:CDS
AGAACATGTTGATTATGGGT+TGG 0.606486 7.4:-93611168 MS.gene54380:CDS
AAGGCGATTAGGATCGATGT+TGG 0.608483 7.4:-93610918 MS.gene54380:CDS
GGAAAATGTGGATGTTGGGA+AGG 0.610345 7.4:-93610793 MS.gene54380:CDS
ATAATCATCCTCTTAACACC+AGG 0.619952 7.4:+93610987 None:intergenic
GTTGCGGCTTTAAGGCCGAA+TGG 0.620496 7.4:-93611275 MS.gene54380:CDS
TTTGGAGATTGACCGTTATG+GGG 0.621026 7.4:-93610859 MS.gene54380:CDS
TATGGGGCATACGATTCAGA+AGG 0.628721 7.4:-93610967 MS.gene54380:CDS
ATAATCAAGATGGTAGACCT+AGG 0.629740 7.4:-93610743 MS.gene54380:CDS
GTGTTAAGAGGATGATTATG+GGG 0.631883 7.4:-93610983 MS.gene54380:CDS
AGATGGTAGACCTAGGCAAG+TGG 0.641426 7.4:-93610736 MS.gene54380:CDS
GTTGGTATGTCGAAAGGATG+CGG 0.642403 7.4:-93610900 MS.gene54380:CDS
TTCCTTCCCCGACAATAAGG+CGG 0.649751 7.4:+93611086 None:intergenic
TCCATTAGAAAATGTTCACG+TGG 0.684935 7.4:-93611345 MS.gene54380:CDS
GGTATGTCGAAAGGATGCGG+TGG 0.743976 7.4:-93610897 MS.gene54380:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTCATCTGATTATATAA+AGG + chr7.4:93611295-93611314 None:intergenic 15.0%
!! TTTATATAATCAGATGAATA+AGG - chr7.4:93611294-93611313 MS.gene54380:CDS 15.0%
! GAAAAATCTATGTAAAGGTT+TGG - chr7.4:93610724-93610743 MS.gene54380:CDS 25.0%
!! TACACAAAATGTTACTGTTT+TGG - chr7.4:93610877-93610896 MS.gene54380:CDS 25.0%
!! TGAAAGAACATGTTGATTAT+GGG - chr7.4:93610936-93610955 MS.gene54380:CDS 25.0%
!! TTGAAAGAACATGTTGATTA+TGG - chr7.4:93610935-93610954 MS.gene54380:CDS 25.0%
!! TTGTTGCTTAATAATCAAGA+TGG - chr7.4:93611355-93611374 MS.gene54380:CDS 25.0%
!!! GTAAAGAACCTTTTTGAAAT+CGG + chr7.4:93610860-93610879 None:intergenic 25.0%
AATAAGGAAAATGTGGATGT+TGG - chr7.4:93611310-93611329 MS.gene54380:CDS 30.0%
ATAAGGAAAATGTGGATGTT+GGG - chr7.4:93611311-93611330 MS.gene54380:CDS 30.0%
ATGTAAAGGTTTGGAAGAAA+AGG - chr7.4:93610733-93610752 MS.gene54380:CDS 30.0%
TCAGATGAATAAGGAAAATG+TGG - chr7.4:93611303-93611322 MS.gene54380:CDS 30.0%
! GATTCAATTTTTGTTCATGG+TGG - chr7.4:93610908-93610927 MS.gene54380:CDS 30.0%
! GGCTTAGAAAATTTTCAGAT+GGG - chr7.4:93611056-93611075 MS.gene54380:CDS 30.0%
! GGTGATTCAATTTTTGTTCA+TGG - chr7.4:93610905-93610924 MS.gene54380:CDS 30.0%
! TGGCTTAGAAAATTTTCAGA+TGG - chr7.4:93611055-93611074 MS.gene54380:CDS 30.0%
!!! AATGTTACTGTTTTGGTAGT+TGG - chr7.4:93610884-93610903 MS.gene54380:CDS 30.0%
ATAATCAAGATGGTAGACCT+AGG - chr7.4:93611365-93611384 MS.gene54380:CDS 35.0%
ATAATCATCCTCTTAACACC+AGG + chr7.4:93611124-93611143 None:intergenic 35.0%
ATTCAGAAGGAAGGGATTAA+TGG - chr7.4:93611154-93611173 MS.gene54380:CDS 35.0%
GAGGTTAGTGATTGGTATAA+GGG - chr7.4:93610977-93610996 MS.gene54380:CDS 35.0%
GGTGTTAAGAGGATGATTAT+GGG - chr7.4:93611124-93611143 MS.gene54380:CDS 35.0%
GTGTTAAGAGGATGATTATG+GGG - chr7.4:93611125-93611144 MS.gene54380:CDS 35.0%
TAATGGTGTCTGCGAAAATA+AGG - chr7.4:93611171-93611190 MS.gene54380:CDS 35.0%
TCCATTAGAAAATGTTCACG+TGG - chr7.4:93610763-93610782 MS.gene54380:CDS 35.0%
TGAGGTTAGTGATTGGTATA+AGG - chr7.4:93610976-93610995 MS.gene54380:CDS 35.0%
TTAATGGTGAGGTTAGTGAT+TGG - chr7.4:93610969-93610988 MS.gene54380:CDS 35.0%
TTAGGGAAGAGTTTCATGAT+GGG - chr7.4:93610798-93610817 MS.gene54380:CDS 35.0%
! AGAACATGTTGATTATGGGT+TGG - chr7.4:93610940-93610959 MS.gene54380:CDS 35.0%
! ATAAGGGTTTGTTTGGTAAC+CGG - chr7.4:93610993-93611012 MS.gene54380:CDS 35.0%
! GATTGGTATAAGGGTTTGTT+TGG - chr7.4:93610986-93611005 MS.gene54380:CDS 35.0%
! TGGTGTTAAGAGGATGATTA+TGG - chr7.4:93611123-93611142 MS.gene54380:CDS 35.0%
!! AAGAGTTTCATGATGGGTTT+AGG - chr7.4:93610804-93610823 MS.gene54380:CDS 35.0%
!! AGAGTTTCATGATGGGTTTA+GGG - chr7.4:93610805-93610824 MS.gene54380:CDS 35.0%
!! TATGGGTTGGAGAAGATTAA+TGG - chr7.4:93610953-93610972 MS.gene54380:CDS 35.0%
!! TTTTGGAGATTGACCGTTAT+GGG - chr7.4:93611248-93611267 MS.gene54380:CDS 35.0%
!!! CTTTTTGAAATCGGTCCATT+CGG + chr7.4:93610851-93610870 None:intergenic 35.0%
ATCGATGTTGGTATGTCGAA+AGG - chr7.4:93611202-93611221 MS.gene54380:CDS 40.0%
GTTAGGGAAGAGTTTCATGA+TGG - chr7.4:93610797-93610816 MS.gene54380:CDS 40.0%
GTTGGAGAAGATTAATGGTG+AGG - chr7.4:93610958-93610977 MS.gene54380:CDS 40.0%
TCAATATCCTCACCCCATAA+CGG + chr7.4:93611264-93611283 None:intergenic 40.0%
TCTGCGAAAATAAGGCGATT+AGG - chr7.4:93611179-93611198 MS.gene54380:CDS 40.0%
TTTGGAGATTGACCGTTATG+GGG - chr7.4:93611249-93611268 MS.gene54380:CDS 40.0%
! CGAATGGACCGATTTCAAAA+AGG - chr7.4:93610849-93610868 MS.gene54380:CDS 40.0%
! GCCACGTGAACATTTTCTAA+TGG + chr7.4:93610767-93610786 None:intergenic 40.0%
!!! GTTTTGGAGATTGACCGTTA+TGG - chr7.4:93611247-93611266 MS.gene54380:CDS 40.0%
AAATGTGGATGTTGGGAAGG+TGG - chr7.4:93611318-93611337 MS.gene54380:CDS 45.0%
AAGGCGATTAGGATCGATGT+TGG - chr7.4:93611190-93611209 MS.gene54380:CDS 45.0%
ACGGTCAATCTCCAAAACCT+CGG + chr7.4:93611245-93611264 None:intergenic 45.0%
ATGTTGGGAAGGTGGAAGAA+GGG - chr7.4:93611326-93611345 MS.gene54380:CDS 45.0%
GGAAAATGTGGATGTTGGGA+AGG - chr7.4:93611315-93611334 MS.gene54380:CDS 45.0%
GGCATACGATTCAGAAGGAA+GGG - chr7.4:93611146-93611165 MS.gene54380:CDS 45.0%
GTTGGTATGTCGAAAGGATG+CGG - chr7.4:93611208-93611227 MS.gene54380:CDS 45.0%
TATGGGGCATACGATTCAGA+AGG - chr7.4:93611141-93611160 MS.gene54380:CDS 45.0%
! CATGTTTTGTCGACGATTCC+TGG - chr7.4:93611103-93611122 MS.gene54380:CDS 45.0%
!! TCATGATGGGTTTAGGGCTA+GGG - chr7.4:93610811-93610830 MS.gene54380:CDS 45.0%
!! TTCATGATGGGTTTAGGGCT+AGG - chr7.4:93610810-93610829 MS.gene54380:CDS 45.0%
AATGTTCACGTGGCGTTTCG+TGG - chr7.4:93610773-93610792 MS.gene54380:CDS 50.0%
ACCTAGGCAAGTGGAAGTCA+AGG - chr7.4:93611381-93611400 MS.gene54380:CDS 50.0%
AGATGGTAGACCTAGGCAAG+TGG - chr7.4:93611372-93611391 MS.gene54380:CDS 50.0%
AGATTGACCGTTATGGGGTG+AGG - chr7.4:93611254-93611273 MS.gene54380:CDS 50.0%
CGGTCAATCTCCAAAACCTC+GGG + chr7.4:93611244-93611263 None:intergenic 50.0%
GACAATAAGGCGGCGAAAAC+CGG + chr7.4:93611015-93611034 None:intergenic 50.0%
GATGTTGGGAAGGTGGAAGA+AGG - chr7.4:93611325-93611344 MS.gene54380:CDS 50.0%
GCATTCCTTCCCCGACAATA+AGG + chr7.4:93611028-93611047 None:intergenic 50.0%
GGGCATACGATTCAGAAGGA+AGG - chr7.4:93611145-93611164 MS.gene54380:CDS 50.0%
GGGGAAGGAATGCACTTGTT+TGG - chr7.4:93611035-93611054 MS.gene54380:CDS 50.0%
TTCCTTCCCCGACAATAAGG+CGG + chr7.4:93611025-93611044 None:intergenic 50.0%
! CGACGATTCCTGGTGTTAAG+AGG - chr7.4:93611113-93611132 MS.gene54380:CDS 50.0%
!! GGTTTTCGCCGCCTTATTGT+CGG - chr7.4:93611014-93611033 MS.gene54380:CDS 50.0%
!! GTTTTCGCCGCCTTATTGTC+GGG - chr7.4:93611015-93611034 MS.gene54380:CDS 50.0%
!! TTTTCGCCGCCTTATTGTCG+GGG - chr7.4:93611016-93611035 MS.gene54380:CDS 50.0%
ACGTGGCGTTTCGTGGTGTT+AGG - chr7.4:93610780-93610799 MS.gene54380:CDS 55.0%
ATGTCGAAAGGATGCGGTGG+TGG - chr7.4:93611214-93611233 MS.gene54380:CDS 55.0%
GCCTTGACTTCCACTTGCCT+AGG + chr7.4:93611385-93611404 None:intergenic 55.0%
GGGAAGGTGGAAGAAGGGTT+TGG - chr7.4:93611331-93611350 MS.gene54380:CDS 55.0%
GGTATGTCGAAAGGATGCGG+TGG - chr7.4:93611211-93611230 MS.gene54380:CDS 55.0%
! CGTGGCGTTTCGTGGTGTTA+GGG - chr7.4:93610781-93610800 MS.gene54380:CDS 55.0%
! TGGGTTTAGGGCTAGGGTTG+CGG - chr7.4:93610817-93610836 MS.gene54380:CDS 55.0%
!! GTTGCGGCTTTAAGGCCGAA+TGG - chr7.4:93610833-93610852 MS.gene54380:CDS 55.0%
!! TGGTGGTTTGCCCGAGGTTT+TGG - chr7.4:93611231-93611250 MS.gene54380:CDS 55.0%
GGGCTAGGGTTGCGGCTTTA+AGG - chr7.4:93610825-93610844 MS.gene54380:CDS 60.0%
! ATGCGGTGGTGGTTTGCCCG+AGG - chr7.4:93611225-93611244 MS.gene54380:CDS 65.0%
! CGCCGCCTTATTGTCGGGGA+AGG - chr7.4:93611020-93611039 MS.gene54380:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 93610722 93611408 93610722 ID=MS.gene54380
chr7.4 mRNA 93610722 93611408 93610722 ID=MS.gene54380.t1;Parent=MS.gene54380
chr7.4 exon 93610722 93611408 93610722 ID=MS.gene54380.t1.exon1;Parent=MS.gene54380.t1
chr7.4 CDS 93610722 93611408 93610722 ID=cds.MS.gene54380.t1;Parent=MS.gene54380.t1
Gene Sequence

>MS.gene54380

ATGAAAAATCTATGTAAAGGTTTGGAAGAAAAGGTTGTAGATCCATTAGAAAATGTTCACGTGGCGTTTCGTGGTGTTAGGGAAGAGTTTCATGATGGGTTTAGGGCTAGGGTTGCGGCTTTAAGGCCGAATGGACCGATTTCAAAAAGGTTCTTTACACAAAATGTTACTGTTTTGGTAGTTGGTGATTCAATTTTTGTTCATGGTGGTTTGTTGAAAGAACATGTTGATTATGGGTTGGAGAAGATTAATGGTGAGGTTAGTGATTGGTATAAGGGTTTGTTTGGTAACCGGTTTTCGCCGCCTTATTGTCGGGGAAGGAATGCACTTGTTTGGCTTAGAAAATTTTCAGATGGGAGTTGTGATTGTTCGAGTCTTGAGCATGTTTTGTCGACGATTCCTGGTGTTAAGAGGATGATTATGGGGCATACGATTCAGAAGGAAGGGATTAATGGTGTCTGCGAAAATAAGGCGATTAGGATCGATGTTGGTATGTCGAAAGGATGCGGTGGTGGTTTGCCCGAGGTTTTGGAGATTGACCGTTATGGGGTGAGGATATTGACATCAAATCCTTTATATAATCAGATGAATAAGGAAAATGTGGATGTTGGGAAGGTGGAAGAAGGGTTTGGATTGTTGCTTAATAATCAAGATGGTAGACCTAGGCAAGTGGAAGTCAAGGCTTAG

Protein sequence

>MS.gene54380.t1

MKNLCKGLEEKVVDPLENVHVAFRGVREEFHDGFRARVAALRPNGPISKRFFTQNVTVLVVGDSIFVHGGLLKEHVDYGLEKINGEVSDWYKGLFGNRFSPPYCRGRNALVWLRKFSDGSCDCSSLEHVLSTIPGVKRMIMGHTIQKEGINGVCENKAIRIDVGMSKGCGGGLPEVLEIDRYGVRILTSNPLYNQMNKENVDVGKVEEGFGLLLNNQDGRPRQVEVKA