Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59215.t1 | XP_003592116.1 | 97.7 | 386 | 9 | 0 | 1 | 386 | 1 | 386 | 5.70E-209 | 736.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59215.t1 | G7ICZ3 | 97.7 | 386 | 9 | 0 | 1 | 386 | 1 | 386 | 4.1e-209 | 736.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene59215 | 0.808429 | 3.11E-50 | -1.69E-46 |
| MS.gene049159 | MS.gene59215 | 0.860213 | 2.42E-63 | -1.69E-46 |
| MS.gene049254 | MS.gene59215 | 0.821408 | 4.10E-53 | -1.69E-46 |
| MS.gene049283 | MS.gene59215 | 0.825192 | 5.36E-54 | -1.69E-46 |
| MS.gene049368 | MS.gene59215 | 0.800146 | 1.65E-48 | -1.69E-46 |
| MS.gene049404 | MS.gene59215 | 0.800847 | 1.19E-48 | -1.69E-46 |
| MS.gene049424 | MS.gene59215 | 0.800649 | 1.31E-48 | -1.69E-46 |
| MS.gene049435 | MS.gene59215 | 0.81189 | 5.58E-51 | -1.69E-46 |
| MS.gene049452 | MS.gene59215 | 0.845135 | 4.90E-59 | -1.69E-46 |
| MS.gene049653 | MS.gene59215 | 0.806089 | 9.74E-50 | -1.69E-46 |
| MS.gene049850 | MS.gene59215 | 0.801847 | 7.42E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene59215 | 0.8674 | 1.41E-65 | -1.69E-46 |
| MS.gene050442 | MS.gene59215 | 0.808742 | 2.67E-50 | -1.69E-46 |
| MS.gene050559 | MS.gene59215 | 0.812184 | 4.82E-51 | -1.69E-46 |
| MS.gene050561 | MS.gene59215 | 0.811444 | 6.98E-51 | -1.69E-46 |
| MS.gene050605 | MS.gene59215 | 0.800248 | 1.58E-48 | -1.69E-46 |
| MS.gene050654 | MS.gene59215 | 0.842035 | 3.31E-58 | -1.69E-46 |
| MS.gene050689 | MS.gene59215 | 0.809457 | 1.87E-50 | -1.69E-46 |
| MS.gene050759 | MS.gene59215 | 0.852771 | 3.71E-61 | -1.69E-46 |
| MS.gene050941 | MS.gene59215 | 0.834729 | 2.53E-56 | -1.69E-46 |
| MS.gene050962 | MS.gene59215 | 0.840743 | 7.23E-58 | -1.69E-46 |
| MS.gene051198 | MS.gene59215 | 0.84247 | 2.54E-58 | -1.69E-46 |
| MS.gene051279 | MS.gene59215 | 0.806281 | 8.88E-50 | -1.69E-46 |
| MS.gene051363 | MS.gene59215 | 0.804201 | 2.42E-49 | -1.69E-46 |
| MS.gene051368 | MS.gene59215 | 0.83969 | 1.36E-57 | -1.69E-46 |
| MS.gene051800 | MS.gene59215 | 0.831373 | 1.73E-55 | -1.69E-46 |
| MS.gene051856 | MS.gene59215 | 0.801886 | 7.29E-49 | -1.69E-46 |
| MS.gene052108 | MS.gene59215 | 0.800461 | 1.43E-48 | -1.69E-46 |
| MS.gene052463 | MS.gene59215 | 0.801879 | 7.31E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene59215 | 0.820565 | 6.42E-53 | -1.69E-46 |
| MS.gene052519 | MS.gene59215 | 0.821795 | 3.34E-53 | -1.69E-46 |
| MS.gene052582 | MS.gene59215 | 0.802079 | 6.65E-49 | -1.69E-46 |
| MS.gene052674 | MS.gene59215 | 0.826027 | 3.40E-54 | -1.69E-46 |
| MS.gene052675 | MS.gene59215 | 0.822935 | 1.81E-53 | -1.69E-46 |
| MS.gene052677 | MS.gene59215 | 0.825551 | 4.41E-54 | -1.69E-46 |
| MS.gene052863 | MS.gene59215 | 0.80769 | 4.47E-50 | -1.69E-46 |
| MS.gene052954 | MS.gene59215 | 0.841792 | 3.83E-58 | -1.69E-46 |
| MS.gene053006 | MS.gene59215 | 0.824981 | 6.01E-54 | -1.69E-46 |
| MS.gene053008 | MS.gene59215 | 0.835098 | 2.04E-56 | -1.69E-46 |
| MS.gene053079 | MS.gene59215 | 0.82877 | 7.48E-55 | -1.69E-46 |
| MS.gene053226 | MS.gene59215 | 0.823451 | 1.38E-53 | -1.69E-46 |
| MS.gene05350 | MS.gene59215 | 0.800751 | 1.24E-48 | -1.69E-46 |
| MS.gene053931 | MS.gene59215 | 0.8436 | 1.27E-58 | -1.69E-46 |
| MS.gene05415 | MS.gene59215 | 0.81718 | 3.77E-52 | -1.69E-46 |
| MS.gene054236 | MS.gene59215 | 0.803743 | 3.01E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene59215 | 0.833812 | 4.30E-56 | -1.69E-46 |
| MS.gene054651 | MS.gene59215 | 0.849717 | 2.70E-60 | -1.69E-46 |
| MS.gene054657 | MS.gene59215 | 0.820862 | 5.48E-53 | -1.69E-46 |
| MS.gene054893 | MS.gene59215 | 0.804019 | 2.64E-49 | -1.69E-46 |
| MS.gene054994 | MS.gene59215 | 0.816141 | 6.45E-52 | -1.69E-46 |
| MS.gene05510 | MS.gene59215 | 0.818799 | 1.62E-52 | -1.69E-46 |
| MS.gene05517 | MS.gene59215 | 0.833525 | 5.07E-56 | -1.69E-46 |
| MS.gene055222 | MS.gene59215 | 0.828218 | 1.02E-54 | -1.69E-46 |
| MS.gene055227 | MS.gene59215 | 0.817442 | 3.29E-52 | -1.69E-46 |
| MS.gene055228 | MS.gene59215 | 0.81918 | 1.33E-52 | -1.69E-46 |
| MS.gene055230 | MS.gene59215 | 0.810109 | 1.36E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene59215 | 0.814503 | 1.49E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene59215 | 0.829448 | 5.12E-55 | -1.69E-46 |
| MS.gene055841 | MS.gene59215 | 0.825093 | 5.66E-54 | -1.69E-46 |
| MS.gene055926 | MS.gene59215 | 0.826461 | 2.68E-54 | -1.69E-46 |
| MS.gene056045 | MS.gene59215 | 0.802162 | 6.39E-49 | -1.69E-46 |
| MS.gene056162 | MS.gene59215 | 0.800916 | 1.15E-48 | -1.69E-46 |
| MS.gene056174 | MS.gene59215 | 0.842518 | 2.46E-58 | -1.69E-46 |
| MS.gene056480 | MS.gene59215 | 0.827017 | 1.97E-54 | -1.69E-46 |
| MS.gene056699 | MS.gene59215 | 0.809277 | 2.05E-50 | -1.69E-46 |
| MS.gene056776 | MS.gene59215 | 0.845925 | 3.00E-59 | -1.69E-46 |
| MS.gene05686 | MS.gene59215 | 0.818494 | 1.90E-52 | -1.69E-46 |
| MS.gene05690 | MS.gene59215 | 0.814326 | 1.63E-51 | -1.69E-46 |
| MS.gene057034 | MS.gene59215 | 0.808152 | 3.56E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene59215 | 0.851898 | 6.58E-61 | -1.69E-46 |
| MS.gene057371 | MS.gene59215 | 0.80313 | 4.04E-49 | -1.69E-46 |
| MS.gene057392 | MS.gene59215 | 0.802206 | 6.26E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene59215 | 0.807983 | 3.87E-50 | -1.69E-46 |
| MS.gene057598 | MS.gene59215 | 0.816659 | 4.93E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene59215 | 0.816249 | 6.10E-52 | -1.69E-46 |
| MS.gene057855 | MS.gene59215 | 0.823053 | 1.70E-53 | -1.69E-46 |
| MS.gene057869 | MS.gene59215 | 0.83844 | 2.87E-57 | -1.69E-46 |
| MS.gene057915 | MS.gene59215 | 0.80894 | 2.42E-50 | -1.69E-46 |
| MS.gene058008 | MS.gene59215 | 0.845181 | 4.77E-59 | -1.69E-46 |
| MS.gene058009 | MS.gene59215 | 0.867954 | 9.35E-66 | -1.69E-46 |
| MS.gene058010 | MS.gene59215 | 0.841468 | 4.66E-58 | -1.69E-46 |
| MS.gene058146 | MS.gene59215 | 0.805238 | 1.47E-49 | -1.69E-46 |
| MS.gene058548 | MS.gene59215 | 0.825101 | 5.63E-54 | -1.69E-46 |
| MS.gene058595 | MS.gene59215 | 0.823917 | 1.07E-53 | -1.69E-46 |
| MS.gene058781 | MS.gene59215 | 0.840988 | 6.24E-58 | -1.69E-46 |
| MS.gene058783 | MS.gene59215 | 0.868067 | 8.60E-66 | -1.69E-46 |
| MS.gene058979 | MS.gene59215 | 0.839754 | 1.31E-57 | -1.69E-46 |
| MS.gene059283 | MS.gene59215 | 0.833177 | 6.20E-56 | -1.69E-46 |
| MS.gene059842 | MS.gene59215 | 0.851574 | 8.12E-61 | -1.69E-46 |
| MS.gene060011 | MS.gene59215 | 0.850233 | 1.94E-60 | -1.69E-46 |
| MS.gene060259 | MS.gene59215 | 0.806055 | 9.90E-50 | -1.69E-46 |
| MS.gene060310 | MS.gene59215 | 0.81185 | 5.70E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene59215 | 0.828714 | 7.71E-55 | -1.69E-46 |
| MS.gene060435 | MS.gene59215 | 0.8008 | 1.22E-48 | -1.69E-46 |
| MS.gene06052 | MS.gene59215 | 0.831095 | 2.03E-55 | -1.69E-46 |
| MS.gene060777 | MS.gene59215 | 0.802451 | 5.58E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene59215 | 0.810959 | 8.89E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene59215 | 0.816448 | 5.50E-52 | -1.69E-46 |
| MS.gene060932 | MS.gene59215 | 0.850723 | 1.41E-60 | -1.69E-46 |
| MS.gene060935 | MS.gene59215 | 0.837636 | 4.63E-57 | -1.69E-46 |
| MS.gene060937 | MS.gene59215 | 0.826528 | 2.58E-54 | -1.69E-46 |
| MS.gene060998 | MS.gene59215 | 0.810232 | 1.28E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59215.t1 | MTR_1g098920 | 98.000 | 450 | 9 | 0 | 1 | 450 | 1 | 450 | 0.0 | 918 |
| MS.gene59215.t1 | MTR_6g035315 | 71.429 | 427 | 111 | 5 | 24 | 445 | 22 | 442 | 0.0 | 543 |
| MS.gene59215.t1 | MTR_2g098080 | 47.696 | 369 | 154 | 9 | 89 | 449 | 79 | 416 | 5.89e-95 | 293 |
| MS.gene59215.t1 | MTR_7g103390 | 46.000 | 250 | 107 | 7 | 10 | 252 | 1 | 229 | 3.01e-59 | 201 |
| MS.gene59215.t1 | MTR_1g061640 | 58.642 | 162 | 59 | 3 | 92 | 252 | 95 | 249 | 6.32e-55 | 190 |
| MS.gene59215.t1 | MTR_1g061640 | 48.193 | 83 | 43 | 0 | 365 | 447 | 400 | 482 | 3.41e-19 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59215.t1 | AT1G21200 | 58.043 | 460 | 161 | 10 | 1 | 447 | 1 | 441 | 3.67e-163 | 468 |
| MS.gene59215.t1 | AT1G21200 | 58.043 | 460 | 161 | 10 | 1 | 447 | 1 | 441 | 3.67e-163 | 468 |
| MS.gene59215.t1 | AT1G76870 | 50.112 | 445 | 151 | 14 | 7 | 445 | 2 | 381 | 8.39e-118 | 350 |
| MS.gene59215.t1 | AT1G76870 | 50.112 | 445 | 151 | 14 | 7 | 445 | 2 | 381 | 8.39e-118 | 350 |
| MS.gene59215.t1 | AT1G76870 | 50.112 | 445 | 151 | 14 | 7 | 445 | 2 | 381 | 8.39e-118 | 350 |
| MS.gene59215.t1 | AT3G10040 | 33.034 | 445 | 214 | 16 | 28 | 447 | 29 | 414 | 3.06e-61 | 206 |
Find 103 sgRNAs with CRISPR-Local
Find 119 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAGGGAGTAGAGGTAAAA+AGG | 0.185988 | 1.3:-68000346 | MS.gene59215:CDS |
| CAAAAGTATTAGCATGATTT+TGG | 0.192898 | 1.3:+67999680 | None:intergenic |
| TGCAGTTAGCTCTCCGTAAT+AGG | 0.247641 | 1.3:-67999867 | MS.gene59215:CDS |
| CAAAATCATGCTAATACTTT+TGG | 0.261782 | 1.3:-67999679 | MS.gene59215:CDS |
| AGTATTTCAATGACAGATTA+TGG | 0.262386 | 1.3:-68000447 | MS.gene59215:CDS |
| TGATTTACCTGGATCAATTC+AGG | 0.278938 | 1.3:-68000601 | MS.gene59215:CDS |
| GTTCTTACCATAATTGTAAT+AGG | 0.279481 | 1.3:-67999927 | MS.gene59215:CDS |
| ATTACAGCTGTTTCTTATAT+CGG | 0.281358 | 1.3:-68000267 | MS.gene59215:CDS |
| CGTGGTGAGGGGCGGTTAAA+TGG | 0.283530 | 1.3:-67999733 | MS.gene59215:CDS |
| AATGCAGCCTATTACAATTA+TGG | 0.286210 | 1.3:+67999920 | None:intergenic |
| AGAGCAAAAGTTACAAATTC+AGG | 0.286726 | 1.3:-67999506 | MS.gene59215:CDS |
| AATTCAATGATCTTAATAAA+AGG | 0.306147 | 1.3:-68000104 | MS.gene59215:CDS |
| AAAACCATCCTCCATAAAAC+TGG | 0.318988 | 1.3:+68000380 | None:intergenic |
| CTCGTATCAGCGTGGACAAA+TGG | 0.372153 | 1.3:-67999620 | MS.gene59215:CDS |
| CAGATTATGGTAAGGGAGAA+AGG | 0.389421 | 1.3:-68000434 | MS.gene59215:CDS |
| TTTGGTAGCTTTGATTTACC+TGG | 0.427242 | 1.3:-68000612 | MS.gene59215:CDS |
| GGTGTTGGTGGTAATTCTTT+TGG | 0.433044 | 1.3:-68000630 | MS.gene59215:CDS |
| TGCATTGAAGATTGCAATGC+AGG | 0.434565 | 1.3:+67999886 | None:intergenic |
| TATCATGATCATCCCTATTA+CGG | 0.438626 | 1.3:+67999854 | None:intergenic |
| AGATTATGGTAAGGGAGAAA+GGG | 0.443869 | 1.3:-68000433 | MS.gene59215:CDS |
| TCGTATCAGCGTGGACAAAT+GGG | 0.448709 | 1.3:-67999619 | MS.gene59215:CDS |
| GCAGTTAGCTCTCCGTAATA+GGG | 0.450748 | 1.3:-67999866 | MS.gene59215:CDS |
| GGGAACTTCTTGTGAGGTTG+TGG | 0.453879 | 1.3:-68000052 | MS.gene59215:CDS |
| TGTGAAGTGGACTGATAATA+TGG | 0.455278 | 1.3:-68000301 | MS.gene59215:CDS |
| TCTGATGGTGTTGGTGGAAG+TGG | 0.459755 | 1.3:-68000231 | MS.gene59215:CDS |
| ACAAATTCAGGCTGAGAAGT+TGG | 0.463771 | 1.3:-67999494 | MS.gene59215:CDS |
| AATTGTGCTTCCCGTGGTGA+GGG | 0.470364 | 1.3:-67999745 | MS.gene59215:CDS |
| TTTGCTCTTCTAACTGAACT+TGG | 0.476208 | 1.3:+67999521 | None:intergenic |
| AAAAGCTTAATGATATGTTA+GGG | 0.479717 | 1.3:-68000077 | MS.gene59215:CDS |
| TTCAATGACAGATTATGGTA+AGG | 0.481181 | 1.3:-68000442 | MS.gene59215:CDS |
| CTTCATCATGATCATCAAGC+TGG | 0.494557 | 1.3:+67999812 | None:intergenic |
| GCAGAGATTTAGCAAGCAAA+AGG | 0.497660 | 1.3:-67999446 | MS.gene59215:CDS |
| CAAGCAAAAGGACCGGGAGT+TGG | 0.497817 | 1.3:-67999434 | MS.gene59215:CDS |
| CGCCTTGGCAGCGTGTGAAG+TGG | 0.500864 | 1.3:-68000314 | MS.gene59215:CDS |
| TCACAAGGAGGAATGATTCA+AGG | 0.506693 | 1.3:-68000654 | MS.gene59215:CDS |
| TTATCTTTCCGAGAAAGAAA+AGG | 0.509656 | 1.3:-68000001 | MS.gene59215:CDS |
| GGCTTTAGAACTGAAGCAAA+AGG | 0.514387 | 1.3:-67999362 | MS.gene59215:CDS |
| GATGGCTTTCCACTCACAAT+GGG | 0.519217 | 1.3:-68000492 | MS.gene59215:CDS |
| AAAAGGGATCGTCGTCGCCT+TGG | 0.519575 | 1.3:-68000329 | MS.gene59215:CDS |
| AGGGTTGTCGCTACATTCAT+CGG | 0.521754 | 1.3:-68000520 | MS.gene59215:CDS |
| TCAATGACAGATTATGGTAA+GGG | 0.522539 | 1.3:-68000441 | MS.gene59215:CDS |
| GAAGCTTGAAAATGAACGTA+TGG | 0.528051 | 1.3:-67999383 | MS.gene59215:CDS |
| CAGCATGGTTACAGAAGCAA+TGG | 0.529621 | 1.3:-67999555 | MS.gene59215:CDS |
| CTTAATGATATGTTAGGGAG+GGG | 0.532863 | 1.3:-68000072 | MS.gene59215:CDS |
| GATTTAGCAAGCAAAAGGAC+CGG | 0.534224 | 1.3:-67999441 | MS.gene59215:CDS |
| ATTTAACCGCCCCTCACCAC+GGG | 0.535675 | 1.3:+67999735 | None:intergenic |
| GGACAAATGGGGCAGTCTGA+TGG | 0.540710 | 1.3:-67999607 | MS.gene59215:CDS |
| AAGTGGAAGAAGGAAATTTG+CGG | 0.541346 | 1.3:-68000214 | MS.gene59215:CDS |
| TTTAAAAGCATCGTGTTCAT+CGG | 0.543314 | 1.3:+67999771 | None:intergenic |
| AATGAGTCTCGGCTTTAACT+AGG | 0.543954 | 1.3:-67999338 | None:intergenic |
| GACTCAGCTTCTCCAACTCC+CGG | 0.544178 | 1.3:+67999422 | None:intergenic |
| TAAATTCCATCTAAAAGCGC+AGG | 0.553826 | 1.3:+68000024 | None:intergenic |
| GCTTAATGATATGTTAGGGA+GGG | 0.553961 | 1.3:-68000073 | MS.gene59215:CDS |
| AGAACTTCTGATGGTGTTGG+TGG | 0.554130 | 1.3:-68000237 | MS.gene59215:CDS |
| GGAATGATTCAAGGTGGTGT+TGG | 0.555541 | 1.3:-68000645 | MS.gene59215:CDS |
| ATTAGCTTGATGTTGGTGAA+TGG | 0.556372 | 1.3:+68000551 | None:intergenic |
| GACGCGGATTAGCTTGATGT+TGG | 0.561054 | 1.3:+68000544 | None:intergenic |
| CAATTGTGCTTCCCGTGGTG+AGG | 0.561935 | 1.3:-67999746 | MS.gene59215:CDS |
| TTCATCATGATCATCAAGCT+GGG | 0.564029 | 1.3:+67999813 | None:intergenic |
| CATCAAGCTAATCCGCGTCA+AGG | 0.564490 | 1.3:-68000540 | MS.gene59215:CDS |
| ATGATTCAAGGTGGTGTTGG+TGG | 0.565750 | 1.3:-68000642 | MS.gene59215:CDS |
| AGCTTAATGATATGTTAGGG+AGG | 0.573431 | 1.3:-68000074 | MS.gene59215:CDS |
| ATCAAGCTAATCCGCGTCAA+GGG | 0.574042 | 1.3:-68000539 | MS.gene59215:CDS |
| AGGGAGGGGAACTTCTTGTG+AGG | 0.575992 | 1.3:-68000058 | MS.gene59215:CDS |
| AAGCAAAAGGAAATGAGTCT+CGG | 0.578972 | 1.3:-67999349 | MS.gene59215:CDS |
| TGTCAAGATTATTTCAACAA+AGG | 0.579146 | 1.3:-67999643 | MS.gene59215:CDS |
| CTCTGATCACAGCTCTGCAA+AGG | 0.583764 | 1.3:+68000468 | None:intergenic |
| ATTTAGCAAGCAAAAGGACC+GGG | 0.584138 | 1.3:-67999440 | MS.gene59215:CDS |
| GTGCTTCCCGTGGTGAGGGG+CGG | 0.584219 | 1.3:-67999741 | MS.gene59215:CDS |
| GTCCACTTCACACGCTGCCA+AGG | 0.587195 | 1.3:+68000312 | None:intergenic |
| CTACATTCATCGGTGCAAGA+TGG | 0.587584 | 1.3:-68000510 | MS.gene59215:CDS |
| GTGTTGGTGGAAGTGGAAGA+AGG | 0.588046 | 1.3:-68000224 | MS.gene59215:CDS |
| AGATGGCTTTCCACTCACAA+TGG | 0.589107 | 1.3:-68000493 | MS.gene59215:CDS |
| TGATGTTGGTGAATGGTATG+AGG | 0.591910 | 1.3:+68000558 | None:intergenic |
| TGATGAACCTGAATTGATCC+AGG | 0.599004 | 1.3:+68000594 | None:intergenic |
| ATTGTGCTTCCCGTGGTGAG+GGG | 0.600048 | 1.3:-67999744 | MS.gene59215:CDS |
| GGTCAACAGGAAGGGAGTAG+AGG | 0.601547 | 1.3:-68000354 | MS.gene59215:CDS |
| TAGAGAAACAAAGATTCAAG+TGG | 0.601786 | 1.3:-67999468 | MS.gene59215:CDS |
| AAAGACAATTGTGCTTCCCG+TGG | 0.602217 | 1.3:-67999751 | MS.gene59215:CDS |
| TGCAAAGGTCCCATTGTGAG+TGG | 0.608379 | 1.3:+68000483 | None:intergenic |
| GATTATGGTAAGGGAGAAAG+GGG | 0.609744 | 1.3:-68000432 | MS.gene59215:CDS |
| AGCTGTTTCTTATATCGGTG+AGG | 0.609938 | 1.3:-68000262 | MS.gene59215:CDS |
| CATTTAACCGCCCCTCACCA+CGG | 0.615478 | 1.3:+67999734 | None:intergenic |
| CGTATCAGCGTGGACAAATG+GGG | 0.619035 | 1.3:-67999618 | MS.gene59215:CDS |
| GTTGGTGAATGGTATGAGGA+TGG | 0.620557 | 1.3:+68000562 | None:intergenic |
| GATTGCAATGCAGGATCATG+TGG | 0.628342 | 1.3:+67999895 | None:intergenic |
| GGTGAGGATAGAACTTCTGA+TGG | 0.630725 | 1.3:-68000246 | MS.gene59215:CDS |
| AGCATGGTTACAGAAGCAAT+GGG | 0.632029 | 1.3:-67999554 | MS.gene59215:CDS |
| TCGAAAGTCATGGCTGAAAG+AGG | 0.632554 | 1.3:-68000159 | MS.gene59215:CDS |
| AAACCATCCTCCATAAAACT+GGG | 0.637742 | 1.3:+68000381 | None:intergenic |
| GATAGAACTTCTGATGGTGT+TGG | 0.639977 | 1.3:-68000240 | MS.gene59215:CDS |
| AAGAAATTGAAACAAAACCA+AGG | 0.641410 | 1.3:-67999703 | MS.gene59215:CDS |
| TGTTTCGCCGCAGCAATGTG+AGG | 0.648167 | 1.3:-68000130 | MS.gene59215:CDS |
| AATTTATCCTCACATTGCTG+CGG | 0.655462 | 1.3:+68000123 | None:intergenic |
| CTGAAAACATGAGAGCAGCA+TGG | 0.667038 | 1.3:-67999570 | MS.gene59215:CDS |
| ATGGATGTGAATTTGTCACA+AGG | 0.679998 | 1.3:-68000669 | MS.gene59215:CDS |
| AACAAAGGCTCGTATCAGCG+TGG | 0.694218 | 1.3:-67999628 | MS.gene59215:CDS |
| TGTAGCGACAACCCTTGACG+CGG | 0.703142 | 1.3:+68000528 | None:intergenic |
| GATGTGAATTTGTCACAAGG+AGG | 0.706028 | 1.3:-68000666 | MS.gene59215:CDS |
| ATTATGGTAAGGGAGAAAGG+GGG | 0.719074 | 1.3:-68000431 | MS.gene59215:CDS |
| GAGATGTGTGTCGAAAGTCA+TGG | 0.729050 | 1.3:-68000169 | MS.gene59215:CDS |
| CAAGGAGGAATGATTCAAGG+TGG | 0.742066 | 1.3:-68000651 | MS.gene59215:CDS |
| AGTGGACTGATAATATGGTG+AGG | 0.762323 | 1.3:-68000296 | MS.gene59215:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTCAATGATCTTAATAAA+AGG | - | chr1.3:67999904-67999923 | MS.gene59215:CDS | 15.0% |
| !! | AAAAAGCTTAATGATATGTT+AGG | - | chr1.3:67999930-67999949 | MS.gene59215:CDS | 20.0% |
| !! | AAAAGCTTAATGATATGTTA+GGG | - | chr1.3:67999931-67999950 | MS.gene59215:CDS | 20.0% |
| ! | AAGAAATTGAAACAAAACCA+AGG | - | chr1.3:68000305-68000324 | MS.gene59215:CDS | 25.0% |
| ! | AGTATTTCAATGACAGATTA+TGG | - | chr1.3:67999561-67999580 | MS.gene59215:CDS | 25.0% |
| ! | GTTCTTACCATAATTGTAAT+AGG | - | chr1.3:68000081-68000100 | MS.gene59215:CDS | 25.0% |
| ! | TGTCAAGATTATTTCAACAA+AGG | - | chr1.3:68000365-68000384 | MS.gene59215:CDS | 25.0% |
| !! | CAAAATCATGCTAATACTTT+TGG | - | chr1.3:68000329-68000348 | MS.gene59215:CDS | 25.0% |
| !!! | ATTACAGCTGTTTCTTATAT+CGG | - | chr1.3:67999741-67999760 | MS.gene59215:CDS | 25.0% |
| !!! | CAAAAGTATTAGCATGATTT+TGG | + | chr1.3:68000331-68000350 | None:intergenic | 25.0% |
| AATGCAGCCTATTACAATTA+TGG | + | chr1.3:68000091-68000110 | None:intergenic | 30.0% | |
| AGAGCAAAAGTTACAAATTC+AGG | - | chr1.3:68000502-68000521 | MS.gene59215:CDS | 30.0% | |
| TAGAGAAACAAAGATTCAAG+TGG | - | chr1.3:68000540-68000559 | MS.gene59215:CDS | 30.0% | |
| TATCATGATCATCCCTATTA+CGG | + | chr1.3:68000157-68000176 | None:intergenic | 30.0% | |
| TCAATGACAGATTATGGTAA+GGG | - | chr1.3:67999567-67999586 | MS.gene59215:CDS | 30.0% | |
| TTATCTTTCCGAGAAAGAAA+AGG | - | chr1.3:68000007-68000026 | MS.gene59215:CDS | 30.0% | |
| TTCAATGACAGATTATGGTA+AGG | - | chr1.3:67999566-67999585 | MS.gene59215:CDS | 30.0% | |
| !! | TTTAAAAGCATCGTGTTCAT+CGG | + | chr1.3:68000240-68000259 | None:intergenic | 30.0% |
| AAAACCATCCTCCATAAAAC+TGG | + | chr1.3:67999631-67999650 | None:intergenic | 35.0% | |
| AAACCATCCTCCATAAAACT+GGG | + | chr1.3:67999630-67999649 | None:intergenic | 35.0% | |
| AAGCAAAAGGAAATGAGTCT+CGG | - | chr1.3:68000659-68000678 | MS.gene59215:CDS | 35.0% | |
| AAGTGGAAGAAGGAAATTTG+CGG | - | chr1.3:67999794-67999813 | MS.gene59215:CDS | 35.0% | |
| AATTTATCCTCACATTGCTG+CGG | + | chr1.3:67999888-67999907 | None:intergenic | 35.0% | |
| AGATTATGGTAAGGGAGAAA+GGG | - | chr1.3:67999575-67999594 | MS.gene59215:CDS | 35.0% | |
| AGCTTAATGATATGTTAGGG+AGG | - | chr1.3:67999934-67999953 | MS.gene59215:CDS | 35.0% | |
| ATGGATGTGAATTTGTCACA+AGG | - | chr1.3:67999339-67999358 | MS.gene59215:CDS | 35.0% | |
| CTTAATGATATGTTAGGGAG+GGG | - | chr1.3:67999936-67999955 | MS.gene59215:CDS | 35.0% | |
| GAAGCTTGAAAATGAACGTA+TGG | - | chr1.3:68000625-68000644 | MS.gene59215:CDS | 35.0% | |
| GCTTAATGATATGTTAGGGA+GGG | - | chr1.3:67999935-67999954 | MS.gene59215:CDS | 35.0% | |
| TGATTTACCTGGATCAATTC+AGG | - | chr1.3:67999407-67999426 | MS.gene59215:CDS | 35.0% | |
| TTCATCATGATCATCAAGCT+GGG | + | chr1.3:68000198-68000217 | None:intergenic | 35.0% | |
| TTTGCTCTTCTAACTGAACT+TGG | + | chr1.3:68000490-68000509 | None:intergenic | 35.0% | |
| ! | AACTTCGTCCTTTTCTTTCT+CGG | + | chr1.3:68000018-68000037 | None:intergenic | 35.0% |
| ! | ATTAGCTTGATGTTGGTGAA+TGG | + | chr1.3:67999460-67999479 | None:intergenic | 35.0% |
| ! | TAAATTCCATCTAAAAGCGC+AGG | + | chr1.3:67999987-68000006 | None:intergenic | 35.0% |
| !! | TATTAGCATGATTTTGGCCT+TGG | + | chr1.3:68000325-68000344 | None:intergenic | 35.0% |
| !! | TGTGAAGTGGACTGATAATA+TGG | - | chr1.3:67999707-67999726 | MS.gene59215:CDS | 35.0% |
| !! | TTTGGTAGCTTTGATTTACC+TGG | - | chr1.3:67999396-67999415 | MS.gene59215:CDS | 35.0% |
| !!! | TTTATGGAGGATGGTTTTGA+TGG | - | chr1.3:67999633-67999652 | MS.gene59215:CDS | 35.0% |
| ACAAATTCAGGCTGAGAAGT+TGG | - | chr1.3:68000514-68000533 | MS.gene59215:CDS | 40.0% | |
| AGCATGGTTACAGAAGCAAT+GGG | - | chr1.3:68000454-68000473 | MS.gene59215:CDS | 40.0% | |
| ATTATGGTAAGGGAGAAAGG+GGG | - | chr1.3:67999577-67999596 | MS.gene59215:CDS | 40.0% | |
| ATTTAGCAAGCAAAAGGACC+GGG | - | chr1.3:68000568-68000587 | MS.gene59215:CDS | 40.0% | |
| CAGATTATGGTAAGGGAGAA+AGG | - | chr1.3:67999574-67999593 | MS.gene59215:CDS | 40.0% | |
| CTTCATCATGATCATCAAGC+TGG | + | chr1.3:68000199-68000218 | None:intergenic | 40.0% | |
| GAAGGGAGTAGAGGTAAAAA+GGG | - | chr1.3:67999663-67999682 | MS.gene59215:CDS | 40.0% | |
| GATGTGAATTTGTCACAAGG+AGG | - | chr1.3:67999342-67999361 | MS.gene59215:CDS | 40.0% | |
| GATTATGGTAAGGGAGAAAG+GGG | - | chr1.3:67999576-67999595 | MS.gene59215:CDS | 40.0% | |
| GATTTAGCAAGCAAAAGGAC+CGG | - | chr1.3:68000567-68000586 | MS.gene59215:CDS | 40.0% | |
| GCAGAGATTTAGCAAGCAAA+AGG | - | chr1.3:68000562-68000581 | MS.gene59215:CDS | 40.0% | |
| GGGGAAAAATTGTACAAGTG+AGG | - | chr1.3:67999596-67999615 | MS.gene59215:CDS | 40.0% | |
| TCACAAGGAGGAATGATTCA+AGG | - | chr1.3:67999354-67999373 | MS.gene59215:CDS | 40.0% | |
| TGATGAACCTGAATTGATCC+AGG | + | chr1.3:67999417-67999436 | None:intergenic | 40.0% | |
| TGCATTGAAGATTGCAATGC+AGG | + | chr1.3:68000125-68000144 | None:intergenic | 40.0% | |
| ! | ATTTGCGGTTTTGCAGAAGA+AGG | - | chr1.3:67999809-67999828 | MS.gene59215:CDS | 40.0% |
| ! | GAAAATCCTGCGCTTTTAGA+TGG | - | chr1.3:67999978-67999997 | MS.gene59215:CDS | 40.0% |
| ! | GATAGAACTTCTGATGGTGT+TGG | - | chr1.3:67999768-67999787 | MS.gene59215:CDS | 40.0% |
| ! | TGATGTTGGTGAATGGTATG+AGG | + | chr1.3:67999453-67999472 | None:intergenic | 40.0% |
| ! | TTTGCGGTTTTGCAGAAGAA+GGG | - | chr1.3:67999810-67999829 | MS.gene59215:CDS | 40.0% |
| ! | TTTTGCAGAAGAAGGGTAAG+TGG | - | chr1.3:67999817-67999836 | MS.gene59215:CDS | 40.0% |
| !! | AGCTGTTTCTTATATCGGTG+AGG | - | chr1.3:67999746-67999765 | MS.gene59215:CDS | 40.0% |
| !! | AGTGGACTGATAATATGGTG+AGG | - | chr1.3:67999712-67999731 | MS.gene59215:CDS | 40.0% |
| !! | GGCTTTAGAACTGAAGCAAA+AGG | - | chr1.3:68000646-68000665 | MS.gene59215:CDS | 40.0% |
| !! | GTTTTGATGGTCAACAGGAA+GGG | - | chr1.3:67999646-67999665 | MS.gene59215:CDS | 40.0% |
| !!! | GGTGTTGGTGGTAATTCTTT+TGG | - | chr1.3:67999378-67999397 | MS.gene59215:CDS | 40.0% |
| AAAGACAATTGTGCTTCCCG+TGG | - | chr1.3:68000257-68000276 | MS.gene59215:CDS | 45.0% | |
| AGATGGCTTTCCACTCACAA+TGG | - | chr1.3:67999515-67999534 | MS.gene59215:CDS | 45.0% | |
| AGGGTTGTCGCTACATTCAT+CGG | - | chr1.3:67999488-67999507 | MS.gene59215:CDS | 45.0% | |
| ATCAAGCTAATCCGCGTCAA+GGG | - | chr1.3:67999469-67999488 | MS.gene59215:CDS | 45.0% | |
| CAAGGAGGAATGATTCAAGG+TGG | - | chr1.3:67999357-67999376 | MS.gene59215:CDS | 45.0% | |
| CAGCATGGTTACAGAAGCAA+TGG | - | chr1.3:68000453-68000472 | MS.gene59215:CDS | 45.0% | |
| CTACATTCATCGGTGCAAGA+TGG | - | chr1.3:67999498-67999517 | MS.gene59215:CDS | 45.0% | |
| CTGAAAACATGAGAGCAGCA+TGG | - | chr1.3:68000438-68000457 | MS.gene59215:CDS | 45.0% | |
| GAGATGTGTGTCGAAAGTCA+TGG | - | chr1.3:67999839-67999858 | MS.gene59215:CDS | 45.0% | |
| GATGGCTTTCCACTCACAAT+GGG | - | chr1.3:67999516-67999535 | MS.gene59215:CDS | 45.0% | |
| GATTGCAATGCAGGATCATG+TGG | + | chr1.3:68000116-68000135 | None:intergenic | 45.0% | |
| GGAAGGGAGTAGAGGTAAAA+AGG | - | chr1.3:67999662-67999681 | MS.gene59215:CDS | 45.0% | |
| GGAATGATTCAAGGTGGTGT+TGG | - | chr1.3:67999363-67999382 | MS.gene59215:CDS | 45.0% | |
| GGTGAGGATAGAACTTCTGA+TGG | - | chr1.3:67999762-67999781 | MS.gene59215:CDS | 45.0% | |
| TCGAAAGTCATGGCTGAAAG+AGG | - | chr1.3:67999849-67999868 | MS.gene59215:CDS | 45.0% | |
| TCGTATCAGCGTGGACAAAT+GGG | - | chr1.3:68000389-68000408 | MS.gene59215:CDS | 45.0% | |
| ! | AGAACTTCTGATGGTGTTGG+TGG | - | chr1.3:67999771-67999790 | MS.gene59215:CDS | 45.0% |
| ! | ATGATTCAAGGTGGTGTTGG+TGG | - | chr1.3:67999366-67999385 | MS.gene59215:CDS | 45.0% |
| ! | CATGCTGCTCTCATGTTTTC+AGG | + | chr1.3:68000440-68000459 | None:intergenic | 45.0% |
| ! | GCAGTTAGCTCTCCGTAATA+GGG | - | chr1.3:68000142-68000161 | MS.gene59215:CDS | 45.0% |
| ! | GTTGGTGAATGGTATGAGGA+TGG | + | chr1.3:67999449-67999468 | None:intergenic | 45.0% |
| ! | TGCAGTTAGCTCTCCGTAAT+AGG | - | chr1.3:68000141-68000160 | MS.gene59215:CDS | 45.0% |
| !! | GGTTTTGATGGTCAACAGGA+AGG | - | chr1.3:67999645-67999664 | MS.gene59215:CDS | 45.0% |
| !!! | GGATGGTTTTGATGGTCAAC+AGG | - | chr1.3:67999641-67999660 | MS.gene59215:CDS | 45.0% |
| AACAAAGGCTCGTATCAGCG+TGG | - | chr1.3:68000380-68000399 | MS.gene59215:CDS | 50.0% | |
| AATTGTGCTTCCCGTGGTGA+GGG | - | chr1.3:68000263-68000282 | MS.gene59215:CDS | 50.0% | |
| CATCAAGCTAATCCGCGTCA+AGG | - | chr1.3:67999468-67999487 | MS.gene59215:CDS | 50.0% | |
| CGTATCAGCGTGGACAAATG+GGG | - | chr1.3:68000390-68000409 | MS.gene59215:CDS | 50.0% | |
| CTCGTATCAGCGTGGACAAA+TGG | - | chr1.3:68000388-68000407 | MS.gene59215:CDS | 50.0% | |
| CTCTGATCACAGCTCTGCAA+AGG | + | chr1.3:67999543-67999562 | None:intergenic | 50.0% | |
| GGGAACTTCTTGTGAGGTTG+TGG | - | chr1.3:67999956-67999975 | MS.gene59215:CDS | 50.0% | |
| TGCAAAGGTCCCATTGTGAG+TGG | + | chr1.3:67999528-67999547 | None:intergenic | 50.0% | |
| ! | GGAAGACGAGCCCAGTTTTA+TGG | - | chr1.3:67999617-67999636 | MS.gene59215:CDS | 50.0% |
| ! | GTGTTGGTGGAAGTGGAAGA+AGG | - | chr1.3:67999784-67999803 | MS.gene59215:CDS | 50.0% |
| !! | AGACGAGCCCAGTTTTATGG+AGG | - | chr1.3:67999620-67999639 | MS.gene59215:CDS | 50.0% |
| !! | GACGCGGATTAGCTTGATGT+TGG | + | chr1.3:67999467-67999486 | None:intergenic | 50.0% |
| !! | GAGCCCAGTTTTATGGAGGA+TGG | - | chr1.3:67999624-67999643 | MS.gene59215:CDS | 50.0% |
| !! | TCTGATGGTGTTGGTGGAAG+TGG | - | chr1.3:67999777-67999796 | MS.gene59215:CDS | 50.0% |
| AAAAGGGATCGTCGTCGCCT+TGG | - | chr1.3:67999679-67999698 | MS.gene59215:CDS | 55.0% | |
| AGGGAGGGGAACTTCTTGTG+AGG | - | chr1.3:67999950-67999969 | MS.gene59215:CDS | 55.0% | |
| ATTGTGCTTCCCGTGGTGAG+GGG | - | chr1.3:68000264-68000283 | MS.gene59215:CDS | 55.0% | |
| ATTTAACCGCCCCTCACCAC+GGG | + | chr1.3:68000276-68000295 | None:intergenic | 55.0% | |
| CAAGCAAAAGGACCGGGAGT+TGG | - | chr1.3:68000574-68000593 | MS.gene59215:CDS | 55.0% | |
| CAATTGTGCTTCCCGTGGTG+AGG | - | chr1.3:68000262-68000281 | MS.gene59215:CDS | 55.0% | |
| CATTTAACCGCCCCTCACCA+CGG | + | chr1.3:68000277-68000296 | None:intergenic | 55.0% | |
| GACTCAGCTTCTCCAACTCC+CGG | + | chr1.3:68000589-68000608 | None:intergenic | 55.0% | |
| GGACAAATGGGGCAGTCTGA+TGG | - | chr1.3:68000401-68000420 | MS.gene59215:CDS | 55.0% | |
| GGTCAACAGGAAGGGAGTAG+AGG | - | chr1.3:67999654-67999673 | MS.gene59215:CDS | 55.0% | |
| TGTAGCGACAACCCTTGACG+CGG | + | chr1.3:67999483-67999502 | None:intergenic | 55.0% | |
| TGTTTCGCCGCAGCAATGTG+AGG | - | chr1.3:67999878-67999897 | MS.gene59215:CDS | 55.0% | |
| CGTGGTGAGGGGCGGTTAAA+TGG | - | chr1.3:68000275-68000294 | MS.gene59215:CDS | 60.0% | |
| GTCCACTTCACACGCTGCCA+AGG | + | chr1.3:67999699-67999718 | None:intergenic | 60.0% | |
| CGCCTTGGCAGCGTGTGAAG+TGG | - | chr1.3:67999694-67999713 | MS.gene59215:CDS | 65.0% | |
| GTGCTTCCCGTGGTGAGGGG+CGG | - | chr1.3:68000267-68000286 | MS.gene59215:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 67999339 | 68000691 | 67999339 | ID=MS.gene59215 |
| chr1.3 | mRNA | 67999339 | 68000691 | 67999339 | ID=MS.gene59215.t1;Parent=MS.gene59215 |
| chr1.3 | exon | 67999339 | 68000691 | 67999339 | ID=MS.gene59215.t1.exon1;Parent=MS.gene59215.t1 |
| chr1.3 | CDS | 67999339 | 68000691 | 67999339 | ID=cds.MS.gene59215.t1;Parent=MS.gene59215.t1 |
| Gene Sequence |
| Protein sequence |
