Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75815.t1 | AFK42793.1 | 98.7 | 300 | 4 | 0 | 1 | 300 | 14 | 313 | 2.40E-147 | 531.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75815.t1 | Q84X53 | 35.9 | 237 | 151 | 1 | 56 | 291 | 33 | 269 | 2.6e-33 | 144.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75815.t1 | G7IIY0 | 98.7 | 300 | 4 | 0 | 1 | 300 | 14 | 313 | 1.7e-147 | 531.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene75815.t1 | TR | mTERF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene75815 | 0.809026 | 2.32E-50 | -1.69E-46 |
| MS.gene049351 | MS.gene75815 | 0.821854 | 3.24E-53 | -1.69E-46 |
| MS.gene049356 | MS.gene75815 | 0.820748 | 5.82E-53 | -1.69E-46 |
| MS.gene049369 | MS.gene75815 | 0.828404 | 9.16E-55 | -1.69E-46 |
| MS.gene049427 | MS.gene75815 | 0.823161 | 1.61E-53 | -1.69E-46 |
| MS.gene049491 | MS.gene75815 | 0.822943 | 1.81E-53 | -1.69E-46 |
| MS.gene049614 | MS.gene75815 | 0.829523 | 4.91E-55 | -1.69E-46 |
| MS.gene049661 | MS.gene75815 | 0.846803 | 1.73E-59 | -1.69E-46 |
| MS.gene049662 | MS.gene75815 | 0.829114 | 6.17E-55 | -1.69E-46 |
| MS.gene049758 | MS.gene75815 | 0.813736 | 2.20E-51 | -1.69E-46 |
| MS.gene049805 | MS.gene75815 | 0.830609 | 2.67E-55 | -1.69E-46 |
| MS.gene050076 | MS.gene75815 | 0.851859 | 6.74E-61 | -1.69E-46 |
| MS.gene050128 | MS.gene75815 | 0.813046 | 3.12E-51 | -1.69E-46 |
| MS.gene050301 | MS.gene75815 | 0.844381 | 7.83E-59 | -1.69E-46 |
| MS.gene050397 | MS.gene75815 | 0.807965 | 3.91E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene75815 | 0.871364 | 7.23E-67 | -1.69E-46 |
| MS.gene05053 | MS.gene75815 | 0.80285 | 4.61E-49 | -1.69E-46 |
| MS.gene050767 | MS.gene75815 | 0.872172 | 3.90E-67 | -1.69E-46 |
| MS.gene050770 | MS.gene75815 | 0.872477 | 3.09E-67 | -1.69E-46 |
| MS.gene05101 | MS.gene75815 | 0.868358 | 6.93E-66 | -1.69E-46 |
| MS.gene05105 | MS.gene75815 | 0.810671 | 1.03E-50 | -1.69E-46 |
| MS.gene051471 | MS.gene75815 | 0.831968 | 1.24E-55 | -1.69E-46 |
| MS.gene051692 | MS.gene75815 | 0.869669 | 2.60E-66 | -1.69E-46 |
| MS.gene051877 | MS.gene75815 | 0.814916 | 1.21E-51 | -1.69E-46 |
| MS.gene052078 | MS.gene75815 | 0.838674 | 2.50E-57 | -1.69E-46 |
| MS.gene052178 | MS.gene75815 | 0.810395 | 1.18E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene75815 | 0.807991 | 3.86E-50 | -1.69E-46 |
| MS.gene052253 | MS.gene75815 | 0.811512 | 6.75E-51 | -1.69E-46 |
| MS.gene052271 | MS.gene75815 | 0.837239 | 5.84E-57 | -1.69E-46 |
| MS.gene052287 | MS.gene75815 | 0.819445 | 1.16E-52 | -1.69E-46 |
| MS.gene052290 | MS.gene75815 | 0.831787 | 1.37E-55 | -1.69E-46 |
| MS.gene052310 | MS.gene75815 | 0.837878 | 4.01E-57 | -1.69E-46 |
| MS.gene052322 | MS.gene75815 | 0.823982 | 1.03E-53 | -1.69E-46 |
| MS.gene052326 | MS.gene75815 | 0.838795 | 2.33E-57 | -1.69E-46 |
| MS.gene052340 | MS.gene75815 | 0.831338 | 1.77E-55 | -1.69E-46 |
| MS.gene052421 | MS.gene75815 | 0.837205 | 5.96E-57 | -1.69E-46 |
| MS.gene052481 | MS.gene75815 | 0.8188 | 1.62E-52 | -1.69E-46 |
| MS.gene052484 | MS.gene75815 | 0.811967 | 5.37E-51 | -1.69E-46 |
| MS.gene052485 | MS.gene75815 | 0.851816 | 6.94E-61 | -1.69E-46 |
| MS.gene053103 | MS.gene75815 | 0.84381 | 1.11E-58 | -1.69E-46 |
| MS.gene053104 | MS.gene75815 | 0.802443 | 5.60E-49 | -1.69E-46 |
| MS.gene053207 | MS.gene75815 | 0.801833 | 7.47E-49 | -1.69E-46 |
| MS.gene053209 | MS.gene75815 | 0.854044 | 1.60E-61 | -1.69E-46 |
| MS.gene053322 | MS.gene75815 | 0.846908 | 1.62E-59 | -1.69E-46 |
| MS.gene053449 | MS.gene75815 | 0.815199 | 1.04E-51 | -1.69E-46 |
| MS.gene053813 | MS.gene75815 | 0.861473 | 1.00E-63 | -1.69E-46 |
| MS.gene053836 | MS.gene75815 | 0.81618 | 6.31E-52 | -1.69E-46 |
| MS.gene054123 | MS.gene75815 | 0.835089 | 2.06E-56 | -1.69E-46 |
| MS.gene054376 | MS.gene75815 | 0.867286 | 1.53E-65 | -1.69E-46 |
| MS.gene05455 | MS.gene75815 | 0.811305 | 7.48E-51 | -1.69E-46 |
| MS.gene054802 | MS.gene75815 | 0.841165 | 5.61E-58 | -1.69E-46 |
| MS.gene054803 | MS.gene75815 | 0.840863 | 6.73E-58 | -1.69E-46 |
| MS.gene054804 | MS.gene75815 | 0.844011 | 9.84E-59 | -1.69E-46 |
| MS.gene05505 | MS.gene75815 | 0.818415 | 1.98E-52 | -1.69E-46 |
| MS.gene055116 | MS.gene75815 | 0.827265 | 1.72E-54 | -1.69E-46 |
| MS.gene055254 | MS.gene75815 | 0.843908 | 1.05E-58 | -1.69E-46 |
| MS.gene055277 | MS.gene75815 | 0.86091 | 1.49E-63 | -1.69E-46 |
| MS.gene055390 | MS.gene75815 | 0.833979 | 3.91E-56 | -1.69E-46 |
| MS.gene055397 | MS.gene75815 | 0.833451 | 5.29E-56 | -1.69E-46 |
| MS.gene055412 | MS.gene75815 | 0.841655 | 4.17E-58 | -1.69E-46 |
| MS.gene055422 | MS.gene75815 | 0.825806 | 3.83E-54 | -1.69E-46 |
| MS.gene055428 | MS.gene75815 | 0.836152 | 1.11E-56 | -1.69E-46 |
| MS.gene055499 | MS.gene75815 | 0.843704 | 1.19E-58 | -1.69E-46 |
| MS.gene055633 | MS.gene75815 | 0.800709 | 1.27E-48 | -1.69E-46 |
| MS.gene055647 | MS.gene75815 | 0.82218 | 2.72E-53 | -1.69E-46 |
| MS.gene055722 | MS.gene75815 | 0.822217 | 2.67E-53 | -1.69E-46 |
| MS.gene055802 | MS.gene75815 | 0.803854 | 2.86E-49 | -1.69E-46 |
| MS.gene05592 | MS.gene75815 | 0.829835 | 4.12E-55 | -1.69E-46 |
| MS.gene056191 | MS.gene75815 | 0.857168 | 1.96E-62 | -1.69E-46 |
| MS.gene056374 | MS.gene75815 | 0.820813 | 5.63E-53 | -1.69E-46 |
| MS.gene056407 | MS.gene75815 | 0.821179 | 4.63E-53 | -1.69E-46 |
| MS.gene056410 | MS.gene75815 | 0.826434 | 2.72E-54 | -1.69E-46 |
| MS.gene056609 | MS.gene75815 | 0.849857 | 2.47E-60 | -1.69E-46 |
| MS.gene056897 | MS.gene75815 | 0.817739 | 2.82E-52 | -1.69E-46 |
| MS.gene057011 | MS.gene75815 | 0.818852 | 1.58E-52 | -1.69E-46 |
| MS.gene05702 | MS.gene75815 | 0.827529 | 1.49E-54 | -1.69E-46 |
| MS.gene057124 | MS.gene75815 | 0.81228 | 4.59E-51 | -1.69E-46 |
| MS.gene057129 | MS.gene75815 | 0.864062 | 1.59E-64 | -1.69E-46 |
| MS.gene057146 | MS.gene75815 | 0.809048 | 2.29E-50 | -1.69E-46 |
| MS.gene057285 | MS.gene75815 | 0.820184 | 7.84E-53 | -1.69E-46 |
| MS.gene057295 | MS.gene75815 | 0.854605 | 1.10E-61 | -1.69E-46 |
| MS.gene05733 | MS.gene75815 | 0.848561 | 5.67E-60 | -1.69E-46 |
| MS.gene057416 | MS.gene75815 | 0.821733 | 3.45E-53 | -1.69E-46 |
| MS.gene057449 | MS.gene75815 | 0.805454 | 1.32E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene75815 | 0.810878 | 9.26E-51 | -1.69E-46 |
| MS.gene057749 | MS.gene75815 | 0.804985 | 1.66E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene75815 | 0.813995 | 1.93E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene75815 | 0.820086 | 8.26E-53 | -1.69E-46 |
| MS.gene058006 | MS.gene75815 | 0.835103 | 2.04E-56 | -1.69E-46 |
| MS.gene058019 | MS.gene75815 | 0.806044 | 9.96E-50 | -1.69E-46 |
| MS.gene05811 | MS.gene75815 | 0.814752 | 1.31E-51 | -1.69E-46 |
| MS.gene058293 | MS.gene75815 | 0.865984 | 3.97E-65 | -1.69E-46 |
| MS.gene058323 | MS.gene75815 | 0.815291 | 9.97E-52 | -1.69E-46 |
| MS.gene05842 | MS.gene75815 | 0.802119 | 6.53E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene75815 | 0.857845 | 1.24E-62 | -1.69E-46 |
| MS.gene058787 | MS.gene75815 | 0.819858 | 9.31E-53 | -1.69E-46 |
| MS.gene058928 | MS.gene75815 | 0.824865 | 6.40E-54 | -1.69E-46 |
| MS.gene05907 | MS.gene75815 | 0.831906 | 1.28E-55 | -1.69E-46 |
| MS.gene059326 | MS.gene75815 | 0.807436 | 5.06E-50 | -1.69E-46 |
| MS.gene059518 | MS.gene75815 | 0.804919 | 1.71E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene75815 | 0.816535 | 5.26E-52 | -1.69E-46 |
| MS.gene059597 | MS.gene75815 | 0.840058 | 1.09E-57 | -1.69E-46 |
| MS.gene05959 | MS.gene75815 | 0.802504 | 5.44E-49 | -1.69E-46 |
| MS.gene059676 | MS.gene75815 | 0.826463 | 2.68E-54 | -1.69E-46 |
| MS.gene059688 | MS.gene75815 | 0.852874 | 3.47E-61 | -1.69E-46 |
| MS.gene059692 | MS.gene75815 | 0.867172 | 1.66E-65 | -1.69E-46 |
| MS.gene059695 | MS.gene75815 | 0.863231 | 2.89E-64 | -1.69E-46 |
| MS.gene059708 | MS.gene75815 | 0.804715 | 1.89E-49 | -1.69E-46 |
| MS.gene059711 | MS.gene75815 | 0.813 | 3.19E-51 | -1.69E-46 |
| MS.gene059894 | MS.gene75815 | 0.847632 | 1.02E-59 | -1.69E-46 |
| MS.gene059960 | MS.gene75815 | 0.825466 | 4.62E-54 | -1.69E-46 |
| MS.gene059974 | MS.gene75815 | 0.833661 | 4.69E-56 | -1.69E-46 |
| MS.gene060005 | MS.gene75815 | 0.82831 | 9.65E-55 | -1.69E-46 |
| MS.gene06037 | MS.gene75815 | 0.862366 | 5.33E-64 | -1.69E-46 |
| MS.gene060403 | MS.gene75815 | 0.824044 | 9.99E-54 | -1.69E-46 |
| MS.gene060469 | MS.gene75815 | 0.808783 | 2.61E-50 | -1.69E-46 |
| MS.gene060804 | MS.gene75815 | 0.822333 | 2.51E-53 | -1.69E-46 |
| MS.gene060818 | MS.gene75815 | 0.821126 | 4.76E-53 | -1.69E-46 |
| MS.gene060837 | MS.gene75815 | 0.819424 | 1.17E-52 | -1.69E-46 |
| MS.gene061041 | MS.gene75815 | 0.812472 | 4.17E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene75815 | 0.855562 | 5.81E-62 | -1.69E-46 |
| MS.gene061051 | MS.gene75815 | -0.835932 | 1.26E-56 | -1.69E-46 |
| MS.gene061200 | MS.gene75815 | 0.85434 | 1.31E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene75815 | MS.gene77650 | PPI |
| MS.gene92189 | MS.gene75815 | PPI |
| MS.gene75815 | MS.gene039043 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75815.t1 | MTR_2g049780 | 98.667 | 300 | 4 | 0 | 1 | 300 | 14 | 313 | 0.0 | 601 |
| MS.gene75815.t1 | MTR_5g041630 | 42.491 | 273 | 151 | 1 | 30 | 296 | 25 | 297 | 8.47e-68 | 214 |
| MS.gene75815.t1 | MTR_2g067230 | 39.076 | 238 | 140 | 2 | 56 | 293 | 69 | 301 | 1.20e-56 | 186 |
| MS.gene75815.t1 | MTR_7g081270 | 40.654 | 214 | 123 | 4 | 81 | 291 | 67 | 279 | 1.07e-41 | 145 |
| MS.gene75815.t1 | MTR_2g034600 | 30.165 | 242 | 154 | 6 | 57 | 297 | 322 | 549 | 3.84e-25 | 105 |
| MS.gene75815.t1 | MTR_2g034600 | 30.165 | 242 | 154 | 6 | 57 | 297 | 326 | 553 | 4.32e-25 | 105 |
| MS.gene75815.t1 | MTR_2g034600 | 30.165 | 242 | 154 | 6 | 57 | 297 | 261 | 488 | 4.35e-25 | 104 |
| MS.gene75815.t1 | MTR_4g070060 | 28.112 | 249 | 170 | 6 | 55 | 296 | 344 | 590 | 1.51e-21 | 95.1 |
| MS.gene75815.t1 | MTR_5g068860 | 26.136 | 264 | 154 | 7 | 69 | 293 | 223 | 484 | 2.94e-19 | 88.2 |
| MS.gene75815.t1 | MTR_6g003960 | 25.000 | 228 | 159 | 3 | 70 | 291 | 366 | 587 | 9.51e-18 | 84.0 |
| MS.gene75815.t1 | MTR_5g094610 | 24.215 | 223 | 154 | 4 | 57 | 268 | 227 | 445 | 5.23e-17 | 81.3 |
| MS.gene75815.t1 | MTR_1g115415 | 25.556 | 270 | 160 | 5 | 63 | 293 | 185 | 452 | 9.04e-17 | 80.9 |
| MS.gene75815.t1 | MTR_4g084630 | 23.600 | 250 | 181 | 4 | 52 | 291 | 69 | 318 | 1.48e-16 | 79.0 |
| MS.gene75815.t1 | MTR_0320s0040 | 35.556 | 90 | 57 | 1 | 56 | 145 | 48 | 136 | 2.70e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75815.t1 | AT2G34620 | 66.212 | 293 | 92 | 3 | 9 | 295 | 3 | 294 | 2.15e-128 | 368 |
| MS.gene75815.t1 | AT2G03050 | 48.101 | 237 | 122 | 1 | 56 | 291 | 32 | 268 | 2.09e-69 | 217 |
| MS.gene75815.t1 | AT2G03050 | 48.101 | 237 | 122 | 1 | 56 | 291 | 33 | 269 | 2.43e-69 | 217 |
| MS.gene75815.t1 | AT2G36000 | 37.037 | 243 | 144 | 4 | 58 | 296 | 75 | 312 | 1.45e-50 | 170 |
| MS.gene75815.t1 | AT2G36000 | 36.364 | 242 | 147 | 3 | 58 | 296 | 75 | 312 | 1.49e-50 | 169 |
| MS.gene75815.t1 | AT3G18870 | 37.705 | 244 | 143 | 4 | 51 | 291 | 31 | 268 | 1.67e-45 | 155 |
| MS.gene75815.t1 | AT2G21710 | 28.340 | 247 | 168 | 6 | 57 | 296 | 353 | 597 | 5.43e-24 | 102 |
| MS.gene75815.t1 | AT4G14605 | 29.787 | 235 | 160 | 5 | 58 | 291 | 237 | 467 | 2.41e-21 | 94.4 |
| MS.gene75815.t1 | AT4G14605 | 26.816 | 179 | 126 | 3 | 101 | 278 | 212 | 386 | 9.36e-13 | 68.9 |
| MS.gene75815.t1 | AT4G14605 | 29.787 | 235 | 160 | 5 | 58 | 291 | 237 | 467 | 2.41e-21 | 94.4 |
| MS.gene75815.t1 | AT4G14605 | 26.816 | 179 | 126 | 3 | 101 | 278 | 212 | 386 | 9.36e-13 | 68.9 |
| MS.gene75815.t1 | AT1G78930 | 25.328 | 229 | 167 | 2 | 65 | 291 | 348 | 574 | 2.44e-19 | 88.6 |
| MS.gene75815.t1 | AT5G55580 | 24.771 | 218 | 160 | 4 | 60 | 273 | 226 | 443 | 2.72e-16 | 79.3 |
| MS.gene75815.t1 | AT5G55580 | 24.771 | 218 | 160 | 4 | 60 | 273 | 120 | 337 | 2.73e-16 | 79.0 |
| MS.gene75815.t1 | AT4G02990 | 26.667 | 270 | 157 | 8 | 63 | 293 | 225 | 492 | 1.07e-15 | 77.8 |
| MS.gene75815.t1 | AT4G02990 | 26.667 | 270 | 157 | 8 | 63 | 293 | 225 | 492 | 1.07e-15 | 77.8 |
| MS.gene75815.t1 | AT4G38160 | 24.506 | 253 | 180 | 4 | 52 | 293 | 70 | 322 | 2.66e-15 | 75.5 |
| MS.gene75815.t1 | AT4G38160 | 24.506 | 253 | 180 | 4 | 52 | 293 | 70 | 322 | 2.66e-15 | 75.5 |
| MS.gene75815.t1 | AT4G38160 | 24.506 | 253 | 180 | 4 | 52 | 293 | 70 | 322 | 2.71e-15 | 75.9 |
| MS.gene75815.t1 | AT4G38160 | 24.506 | 253 | 180 | 4 | 52 | 293 | 70 | 322 | 4.29e-15 | 75.1 |
| MS.gene75815.t1 | AT2G44020 | 25.481 | 208 | 119 | 2 | 86 | 293 | 286 | 457 | 6.71e-15 | 75.1 |
| MS.gene75815.t1 | AT2G21710 | 25.243 | 206 | 145 | 6 | 57 | 255 | 353 | 556 | 5.46e-13 | 69.7 |
| MS.gene75815.t1 | AT2G21710 | 24.878 | 205 | 145 | 6 | 57 | 254 | 353 | 555 | 4.33e-12 | 67.0 |
Find 60 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTAAGCATTTGGAGAGTA+TGG | 0.202455 | 2.1:+42466120 | MS.gene75815:CDS |
| AAAGTACTGATGATGAATTT+AGG | 0.234140 | 2.1:+42466380 | MS.gene75815:CDS |
| TTACCAAGATTGTGTTCTTT+TGG | 0.237636 | 2.1:+42466066 | MS.gene75815:CDS |
| CTGTATGAAGGTTAGGGTTT+TGG | 0.245384 | 2.1:-42465751 | None:intergenic |
| ATGCTTGAAGTGAGAATCTT+AGG | 0.252141 | 2.1:-42465863 | None:intergenic |
| TTTGGGTTTGGGAGTTAAAT+TGG | 0.262027 | 2.1:-42465612 | None:intergenic |
| GAAGTAATGGGCATTGATTC+AGG | 0.276282 | 2.1:+42465716 | MS.gene75815:CDS |
| TGTGGAAATGAAGGGGATAT+TGG | 0.279926 | 2.1:+42466243 | MS.gene75815:CDS |
| AAGTACTGATGATGAATTTA+GGG | 0.305836 | 2.1:+42466381 | MS.gene75815:CDS |
| GTCTTTATGTTGGATACCTT+TGG | 0.310081 | 2.1:-42465816 | None:intergenic |
| GGACACATGCCAAAGATTCT+TGG | 0.310114 | 2.1:-42465842 | None:intergenic |
| AAAGACCTTCCAAGAATCTT+TGG | 0.314251 | 2.1:+42465833 | MS.gene75815:CDS |
| AATGTAGCTGTATGAAGGTT+AGG | 0.329816 | 2.1:-42465758 | None:intergenic |
| TATTCCTAAACTTAAGCATT+TGG | 0.349186 | 2.1:+42466111 | MS.gene75815:CDS |
| CTCTCCAAATGCTTAAGTTT+AGG | 0.351147 | 2.1:-42466115 | None:intergenic |
| ATTCTTTGTCTTGAAGTAAT+GGG | 0.356794 | 2.1:+42465704 | MS.gene75815:CDS |
| GGAAACAAGAAAGGTTATAA+TGG | 0.358255 | 2.1:-42465795 | None:intergenic |
| TAGTTTGGAGAATAGGATAA+AGG | 0.362241 | 2.1:+42466300 | MS.gene75815:CDS |
| GATTCTTTGTCTTGAAGTAA+TGG | 0.416623 | 2.1:+42465703 | MS.gene75815:CDS |
| ACACTAGAAGTAAGCAATCT+TGG | 0.430968 | 2.1:-42465974 | None:intergenic |
| AAAGGTTATAATGGAATGAA+TGG | 0.434056 | 2.1:-42465786 | None:intergenic |
| ATTAAGAGATTTAGAAGCAT+TGG | 0.444652 | 2.1:+42466042 | MS.gene75815:CDS |
| GATACCTTTGGAAACAAGAA+AGG | 0.447305 | 2.1:-42465804 | None:intergenic |
| TTTAGGGAGTTGATAAAGAA+GGG | 0.454349 | 2.1:+42466397 | MS.gene75815:CDS |
| AGAGCTATGGTTCTAGCAGC+TGG | 0.458017 | 2.1:-42465533 | None:intergenic |
| AATAAGTGTTCTTTCAACAT+TGG | 0.460379 | 2.1:-42466092 | None:intergenic |
| GGTTAGACACATGGAGGTTG+TGG | 0.463307 | 2.1:+42466321 | MS.gene75815:CDS |
| TTCTTGGAAGGTCTTTATGT+TGG | 0.468294 | 2.1:-42465826 | None:intergenic |
| ATGTAGCTGTATGAAGGTTA+GGG | 0.471857 | 2.1:-42465757 | None:intergenic |
| CACATGGAGGTTGTGGAAAG+TGG | 0.473398 | 2.1:+42466328 | MS.gene75815:CDS |
| TTGGTTGAAGGAGGTGATGA+TGG | 0.480821 | 2.1:-42465593 | None:intergenic |
| TAAAGAAGAGGCTAGGTGTA+TGG | 0.485234 | 2.1:+42466150 | MS.gene75815:CDS |
| CTTAAGCATTTGGAGAGTAT+GGG | 0.487893 | 2.1:+42466121 | MS.gene75815:CDS |
| AAACCAAAAGAACACAATCT+TGG | 0.492208 | 2.1:-42466069 | None:intergenic |
| ATTTAGGGAGTTGATAAAGA+AGG | 0.499226 | 2.1:+42466396 | MS.gene75815:CDS |
| TTGGGAGTTAAATTGGTTGA+AGG | 0.501320 | 2.1:-42465605 | None:intergenic |
| GGAGTTAAATTGGTTGAAGG+AGG | 0.504389 | 2.1:-42465602 | None:intergenic |
| TCATGAGTTGGTGTGTAAAG+TGG | 0.520744 | 2.1:-42465653 | None:intergenic |
| ATAACCTTTCTTGTTTCCAA+AGG | 0.524436 | 2.1:+42465800 | MS.gene75815:CDS |
| ATCAGATGAGACTGAACATA+GGG | 0.547332 | 2.1:-42465567 | None:intergenic |
| AGGCTAGGTGTATGGTGTTG+AGG | 0.553273 | 2.1:+42466158 | MS.gene75815:CDS |
| ATTCTAATGTAGCTGTATGA+AGG | 0.553387 | 2.1:-42465763 | None:intergenic |
| ATTGCTTACTTCTAGTGTGG+TGG | 0.553869 | 2.1:+42465979 | MS.gene75815:CDS |
| CTGTTAAGATAAAACAAAGC+AGG | 0.557661 | 2.1:-42466013 | None:intergenic |
| ATACTAAAAGTCAACAATGC+AGG | 0.557976 | 2.1:-42466184 | None:intergenic |
| AAGATTGCTTACTTCTAGTG+TGG | 0.564951 | 2.1:+42465976 | MS.gene75815:CDS |
| GGAAGATGAGTGTAGAGCTA+TGG | 0.568612 | 2.1:-42465546 | None:intergenic |
| GGAAATGAAGGGGATATTGG+AGG | 0.569285 | 2.1:+42466246 | MS.gene75815:CDS |
| TAGGGAGTTGATAAAGAAGG+GGG | 0.580226 | 2.1:+42466399 | MS.gene75815:CDS |
| ACATGGAGGTTGTGGAAAGT+GGG | 0.587398 | 2.1:+42466329 | MS.gene75815:CDS |
| GAGCTATGGTTCTAGCAGCT+GGG | 0.599605 | 2.1:-42465532 | None:intergenic |
| TATGGGATTTACTAAAGAAG+AGG | 0.599972 | 2.1:+42466138 | MS.gene75815:CDS |
| TATCAGATGAGACTGAACAT+AGG | 0.607408 | 2.1:-42465568 | None:intergenic |
| TTAGGGAGTTGATAAAGAAG+GGG | 0.610325 | 2.1:+42466398 | MS.gene75815:CDS |
| GAGTTGGTGTGTAAAGTGGA+TGG | 0.622825 | 2.1:-42465649 | None:intergenic |
| GATTTACTAAAGAAGAGGCT+AGG | 0.635507 | 2.1:+42466143 | MS.gene75815:CDS |
| TAGGATAAAGGTTAGACACA+TGG | 0.648833 | 2.1:+42466312 | MS.gene75815:CDS |
| ACATGCCAAAGATTCTTGGA+AGG | 0.652767 | 2.1:-42465838 | None:intergenic |
| AAACTAAAAGAGAAATACTG+AGG | 0.668925 | 2.1:-42466283 | None:intergenic |
| GATAAAGGTTAGACACATGG+AGG | 0.698797 | 2.1:+42466315 | MS.gene75815:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAGAAAAATACTCAAATTT+AGG | - | chr2.1:42466226-42466245 | None:intergenic | 20.0% |
| !!! | TAAATTTGAGTATTTTTCTG+TGG | + | chr2.1:42466225-42466244 | MS.gene75815:CDS | 20.0% |
| !!! | TTGTTTTATCTTAACAGATT+AGG | + | chr2.1:42466019-42466038 | MS.gene75815:CDS | 20.0% |
| ! | AAACTAAAAGAGAAATACTG+AGG | - | chr2.1:42466286-42466305 | None:intergenic | 25.0% |
| ! | AATAAGTGTTCTTTCAACAT+TGG | - | chr2.1:42466095-42466114 | None:intergenic | 25.0% |
| ! | ATTCTTTGTCTTGAAGTAAT+GGG | + | chr2.1:42465704-42465723 | MS.gene75815:CDS | 25.0% |
| ! | TATTCCTAAACTTAAGCATT+TGG | + | chr2.1:42466111-42466130 | MS.gene75815:CDS | 25.0% |
| ! | TGTATGAGAAAATCAAAAAC+TGG | - | chr2.1:42465905-42465924 | None:intergenic | 25.0% |
| !! | AAAGGTTATAATGGAATGAA+TGG | - | chr2.1:42465789-42465808 | None:intergenic | 25.0% |
| !! | ATTAAGAGATTTAGAAGCAT+TGG | + | chr2.1:42466042-42466061 | MS.gene75815:CDS | 25.0% |
| !! | TATTTTTCTGTGGAAATGAA+GGG | + | chr2.1:42466235-42466254 | MS.gene75815:CDS | 25.0% |
| !!! | AAAGTACTGATGATGAATTT+AGG | + | chr2.1:42466380-42466399 | MS.gene75815:CDS | 25.0% |
| !!! | AAGTACTGATGATGAATTTA+GGG | + | chr2.1:42466381-42466400 | MS.gene75815:CDS | 25.0% |
| !!! | TCAGTATTTCTCTTTTAGTT+TGG | + | chr2.1:42466285-42466304 | MS.gene75815:CDS | 25.0% |
| AAACCAAAAGAACACAATCT+TGG | - | chr2.1:42466072-42466091 | None:intergenic | 30.0% | |
| ATACTAAAAGTCAACAATGC+AGG | - | chr2.1:42466187-42466206 | None:intergenic | 30.0% | |
| ATTCTAATGTAGCTGTATGA+AGG | - | chr2.1:42465766-42465785 | None:intergenic | 30.0% | |
| CTGTTAAGATAAAACAAAGC+AGG | - | chr2.1:42466016-42466035 | None:intergenic | 30.0% | |
| GATTCTTTGTCTTGAAGTAA+TGG | + | chr2.1:42465703-42465722 | MS.gene75815:CDS | 30.0% | |
| GGAAACAAGAAAGGTTATAA+TGG | - | chr2.1:42465798-42465817 | None:intergenic | 30.0% | |
| TAGTTTGGAGAATAGGATAA+AGG | + | chr2.1:42466300-42466319 | MS.gene75815:CDS | 30.0% | |
| TATGGGATTTACTAAAGAAG+AGG | + | chr2.1:42466138-42466157 | MS.gene75815:CDS | 30.0% | |
| ! | ATAACCTTTCTTGTTTCCAA+AGG | + | chr2.1:42465800-42465819 | MS.gene75815:CDS | 30.0% |
| ! | ATTTTTCTGTGGAAATGAAG+GGG | + | chr2.1:42466236-42466255 | MS.gene75815:CDS | 30.0% |
| ! | CTTTTAAGCATCAATGACAA+AGG | - | chr2.1:42466364-42466383 | None:intergenic | 30.0% |
| ! | GTATTTTTCTGTGGAAATGA+AGG | + | chr2.1:42466234-42466253 | MS.gene75815:CDS | 30.0% |
| ! | TTACCAAGATTGTGTTCTTT+TGG | + | chr2.1:42466066-42466085 | MS.gene75815:CDS | 30.0% |
| !! | ATTTAGGGAGTTGATAAAGA+AGG | + | chr2.1:42466396-42466415 | MS.gene75815:CDS | 30.0% |
| !! | TTTAGGGAGTTGATAAAGAA+GGG | + | chr2.1:42466397-42466416 | MS.gene75815:CDS | 30.0% |
| !!! | ACTTTTCTAAAGCTATGATC+AGG | - | chr2.1:42465941-42465960 | None:intergenic | 30.0% |
| !!! | TCTCTTTTAGTTTGGAGAAT+AGG | + | chr2.1:42466293-42466312 | MS.gene75815:CDS | 30.0% |
| AAAGACCTTCCAAGAATCTT+TGG | + | chr2.1:42465833-42465852 | MS.gene75815:CDS | 35.0% | |
| AAGATTGCTTACTTCTAGTG+TGG | + | chr2.1:42465976-42465995 | MS.gene75815:CDS | 35.0% | |
| AATGTAGCTGTATGAAGGTT+AGG | - | chr2.1:42465761-42465780 | None:intergenic | 35.0% | |
| ACACTAGAAGTAAGCAATCT+TGG | - | chr2.1:42465977-42465996 | None:intergenic | 35.0% | |
| ACTTAAGCATTTGGAGAGTA+TGG | + | chr2.1:42466120-42466139 | MS.gene75815:CDS | 35.0% | |
| ATCAGATGAGACTGAACATA+GGG | - | chr2.1:42465570-42465589 | None:intergenic | 35.0% | |
| ATGCTTGAAGTGAGAATCTT+AGG | - | chr2.1:42465866-42465885 | None:intergenic | 35.0% | |
| ATGTAGCTGTATGAAGGTTA+GGG | - | chr2.1:42465760-42465779 | None:intergenic | 35.0% | |
| CTCTCCAAATGCTTAAGTTT+AGG | - | chr2.1:42466118-42466137 | None:intergenic | 35.0% | |
| GATTTACTAAAGAAGAGGCT+AGG | + | chr2.1:42466143-42466162 | MS.gene75815:CDS | 35.0% | |
| GTCTTTATGTTGGATACCTT+TGG | - | chr2.1:42465819-42465838 | None:intergenic | 35.0% | |
| TAGGATAAAGGTTAGACACA+TGG | + | chr2.1:42466312-42466331 | MS.gene75815:CDS | 35.0% | |
| TATCAGATGAGACTGAACAT+AGG | - | chr2.1:42465571-42465590 | None:intergenic | 35.0% | |
| TTCTTGGAAGGTCTTTATGT+TGG | - | chr2.1:42465829-42465848 | None:intergenic | 35.0% | |
| TTGGGAGTTAAATTGGTTGA+AGG | - | chr2.1:42465608-42465627 | None:intergenic | 35.0% | |
| ! | AGTGAGAGTTTTTCATGAGT+TGG | - | chr2.1:42465668-42465687 | None:intergenic | 35.0% |
| ! | CTTAAGCATTTGGAGAGTAT+GGG | + | chr2.1:42466121-42466140 | MS.gene75815:CDS | 35.0% |
| ! | GATACCTTTGGAAACAAGAA+AGG | - | chr2.1:42465807-42465826 | None:intergenic | 35.0% |
| ! | TGTATGAAGGTTAGGGTTTT+GGG | - | chr2.1:42465753-42465772 | None:intergenic | 35.0% |
| ! | TTTGGGTTTGGGAGTTAAAT+TGG | - | chr2.1:42465615-42465634 | None:intergenic | 35.0% |
| !! | TTAGGGAGTTGATAAAGAAG+GGG | + | chr2.1:42466398-42466417 | MS.gene75815:CDS | 35.0% |
| !!! | ATGGTTTTGAAGGAGACTTT+TGG | - | chr2.1:42465633-42465652 | None:intergenic | 35.0% |
| !!! | TGGTTTTGAAGGAGACTTTT+GGG | - | chr2.1:42465632-42465651 | None:intergenic | 35.0% |
| !!! | TGTAAAGTGGATGGTTTTGA+AGG | - | chr2.1:42465643-42465662 | None:intergenic | 35.0% |
| ACATGCCAAAGATTCTTGGA+AGG | - | chr2.1:42465841-42465860 | None:intergenic | 40.0% | |
| ATTGCTTACTTCTAGTGTGG+TGG | + | chr2.1:42465979-42465998 | MS.gene75815:CDS | 40.0% | |
| GATAAAGGTTAGACACATGG+AGG | + | chr2.1:42466315-42466334 | MS.gene75815:CDS | 40.0% | |
| GGAGTTAAATTGGTTGAAGG+AGG | - | chr2.1:42465605-42465624 | None:intergenic | 40.0% | |
| TAAAGAAGAGGCTAGGTGTA+TGG | + | chr2.1:42466150-42466169 | MS.gene75815:CDS | 40.0% | |
| TGTGGAAATGAAGGGGATAT+TGG | + | chr2.1:42466243-42466262 | MS.gene75815:CDS | 40.0% | |
| ! | CTGTATGAAGGTTAGGGTTT+TGG | - | chr2.1:42465754-42465773 | None:intergenic | 40.0% |
| !! | GAAGTAATGGGCATTGATTC+AGG | + | chr2.1:42465716-42465735 | MS.gene75815:CDS | 40.0% |
| !! | TAGGGAGTTGATAAAGAAGG+GGG | + | chr2.1:42466399-42466418 | MS.gene75815:CDS | 40.0% |
| !! | TCATGAGTTGGTGTGTAAAG+TGG | - | chr2.1:42465656-42465675 | None:intergenic | 40.0% |
| !!! | TGAAGGAGACTTTTGGGTTT+GGG | - | chr2.1:42465626-42465645 | None:intergenic | 40.0% |
| !!! | TTGAAGGAGACTTTTGGGTT+TGG | - | chr2.1:42465627-42465646 | None:intergenic | 40.0% |
| ACATGGAGGTTGTGGAAAGT+GGG | + | chr2.1:42466329-42466348 | MS.gene75815:CDS | 45.0% | |
| GGAAATGAAGGGGATATTGG+AGG | + | chr2.1:42466246-42466265 | MS.gene75815:CDS | 45.0% | |
| GGAAGATGAGTGTAGAGCTA+TGG | - | chr2.1:42465549-42465568 | None:intergenic | 45.0% | |
| GGACACATGCCAAAGATTCT+TGG | - | chr2.1:42465845-42465864 | None:intergenic | 45.0% | |
| TTGGTTGAAGGAGGTGATGA+TGG | - | chr2.1:42465596-42465615 | None:intergenic | 45.0% | |
| !! | GAGTTGGTGTGTAAAGTGGA+TGG | - | chr2.1:42465652-42465671 | None:intergenic | 45.0% |
| CACATGGAGGTTGTGGAAAG+TGG | + | chr2.1:42466328-42466347 | MS.gene75815:CDS | 50.0% | |
| GGTTAGACACATGGAGGTTG+TGG | + | chr2.1:42466321-42466340 | MS.gene75815:CDS | 50.0% | |
| ! | AGGCTAGGTGTATGGTGTTG+AGG | + | chr2.1:42466158-42466177 | MS.gene75815:CDS | 50.0% |
| !! | AGAGCTATGGTTCTAGCAGC+TGG | - | chr2.1:42465536-42465555 | None:intergenic | 50.0% |
| !! | GAGCTATGGTTCTAGCAGCT+GGG | - | chr2.1:42465535-42465554 | None:intergenic | 50.0% |
| !! | GAGTTGATAAAGAAGGGGGC+TGG | + | chr2.1:42466403-42466422 | MS.gene75815:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 42465527 | 42466429 | 42465527 | ID=MS.gene75815 |
| chr2.1 | mRNA | 42465527 | 42466429 | 42465527 | ID=MS.gene75815.t1;Parent=MS.gene75815 |
| chr2.1 | exon | 42465527 | 42466429 | 42465527 | ID=MS.gene75815.t1.exon1;Parent=MS.gene75815.t1 |
| chr2.1 | CDS | 42465527 | 42466429 | 42465527 | ID=cds.MS.gene75815.t1;Parent=MS.gene75815.t1 |
| Gene Sequence |
| Protein sequence |
