Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78067.t1 | XP_013455324.1 | 92.7 | 123 | 9 | 0 | 1 | 123 | 30 | 152 | 1.10E-53 | 219.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78067.t1 | F4KDA6 | 32.2 | 143 | 75 | 3 | 1 | 123 | 1 | 141 | 1.5e-06 | 53.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78067.t1 | A0A072UU36 | 92.7 | 123 | 9 | 0 | 1 | 123 | 30 | 152 | 8.1e-54 | 219.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049173 | MS.gene78067 | 0.815188 | 1.05E-51 | -1.69E-46 |
| MS.gene049217 | MS.gene78067 | 0.807025 | 6.18E-50 | -1.69E-46 |
| MS.gene049222 | MS.gene78067 | 0.813627 | 2.33E-51 | -1.69E-46 |
| MS.gene049285 | MS.gene78067 | 0.810677 | 1.02E-50 | -1.69E-46 |
| MS.gene049369 | MS.gene78067 | 0.819088 | 1.40E-52 | -1.69E-46 |
| MS.gene049427 | MS.gene78067 | 0.852472 | 4.51E-61 | -1.69E-46 |
| MS.gene049491 | MS.gene78067 | 0.813243 | 2.83E-51 | -1.69E-46 |
| MS.gene049609 | MS.gene78067 | 0.833099 | 6.48E-56 | -1.69E-46 |
| MS.gene049614 | MS.gene78067 | 0.821816 | 3.30E-53 | -1.69E-46 |
| MS.gene049805 | MS.gene78067 | 0.853395 | 2.46E-61 | -1.69E-46 |
| MS.gene050333 | MS.gene78067 | 0.813249 | 2.82E-51 | -1.69E-46 |
| MS.gene050432 | MS.gene78067 | 0.807354 | 5.27E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene78067 | 0.828704 | 7.75E-55 | -1.69E-46 |
| MS.gene050747 | MS.gene78067 | 0.819946 | 8.89E-53 | -1.69E-46 |
| MS.gene050767 | MS.gene78067 | 0.802233 | 6.18E-49 | -1.69E-46 |
| MS.gene050770 | MS.gene78067 | 0.807184 | 5.72E-50 | -1.69E-46 |
| MS.gene050772 | MS.gene78067 | 0.84556 | 3.76E-59 | -1.69E-46 |
| MS.gene050950 | MS.gene78067 | 0.815813 | 7.63E-52 | -1.69E-46 |
| MS.gene05105 | MS.gene78067 | 0.822696 | 2.06E-53 | -1.69E-46 |
| MS.gene051228 | MS.gene78067 | 0.826053 | 3.35E-54 | -1.69E-46 |
| MS.gene051387 | MS.gene78067 | 0.811226 | 7.78E-51 | -1.69E-46 |
| MS.gene051666 | MS.gene78067 | 0.825555 | 4.40E-54 | -1.69E-46 |
| MS.gene051692 | MS.gene78067 | 0.842768 | 2.11E-58 | -1.69E-46 |
| MS.gene051694 | MS.gene78067 | 0.809203 | 2.13E-50 | -1.69E-46 |
| MS.gene051810 | MS.gene78067 | 0.810573 | 1.08E-50 | -1.69E-46 |
| MS.gene051858 | MS.gene78067 | 0.849448 | 3.21E-60 | -1.69E-46 |
| MS.gene052271 | MS.gene78067 | 0.805971 | 1.03E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene78067 | 0.825172 | 5.42E-54 | -1.69E-46 |
| MS.gene052379 | MS.gene78067 | 0.832953 | 7.04E-56 | -1.69E-46 |
| MS.gene052421 | MS.gene78067 | 0.823226 | 1.55E-53 | -1.69E-46 |
| MS.gene052481 | MS.gene78067 | 0.84303 | 1.80E-58 | -1.69E-46 |
| MS.gene052484 | MS.gene78067 | 0.869185 | 3.74E-66 | -1.69E-46 |
| MS.gene052485 | MS.gene78067 | 0.817115 | 3.90E-52 | -1.69E-46 |
| MS.gene052615 | MS.gene78067 | 0.831348 | 1.76E-55 | -1.69E-46 |
| MS.gene052870 | MS.gene78067 | 0.828412 | 9.12E-55 | -1.69E-46 |
| MS.gene052872 | MS.gene78067 | 0.822948 | 1.80E-53 | -1.69E-46 |
| MS.gene053103 | MS.gene78067 | 0.848131 | 7.45E-60 | -1.69E-46 |
| MS.gene053104 | MS.gene78067 | 0.846831 | 1.70E-59 | -1.69E-46 |
| MS.gene053207 | MS.gene78067 | 0.812479 | 4.15E-51 | -1.69E-46 |
| MS.gene053209 | MS.gene78067 | 0.816105 | 6.57E-52 | -1.69E-46 |
| MS.gene053213 | MS.gene78067 | 0.814298 | 1.65E-51 | -1.69E-46 |
| MS.gene053286 | MS.gene78067 | 0.806349 | 8.59E-50 | -1.69E-46 |
| MS.gene053322 | MS.gene78067 | 0.864674 | 1.03E-64 | -1.69E-46 |
| MS.gene053384 | MS.gene78067 | 0.818136 | 2.29E-52 | -1.69E-46 |
| MS.gene053449 | MS.gene78067 | 0.835613 | 1.52E-56 | -1.69E-46 |
| MS.gene053574 | MS.gene78067 | 0.822896 | 1.85E-53 | -1.69E-46 |
| MS.gene053824 | MS.gene78067 | 0.801653 | 8.14E-49 | -1.69E-46 |
| MS.gene053912 | MS.gene78067 | 0.82305 | 1.71E-53 | -1.69E-46 |
| MS.gene054408 | MS.gene78067 | 0.811271 | 7.61E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene78067 | 0.810157 | 1.33E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene78067 | 0.81809 | 2.35E-52 | -1.69E-46 |
| MS.gene054955 | MS.gene78067 | 0.800321 | 1.52E-48 | -1.69E-46 |
| MS.gene05505 | MS.gene78067 | 0.842943 | 1.90E-58 | -1.69E-46 |
| MS.gene055206 | MS.gene78067 | 0.830128 | 3.50E-55 | -1.69E-46 |
| MS.gene055212 | MS.gene78067 | 0.804408 | 2.19E-49 | -1.69E-46 |
| MS.gene055289 | MS.gene78067 | 0.801419 | 9.09E-49 | -1.69E-46 |
| MS.gene055397 | MS.gene78067 | 0.803645 | 3.16E-49 | -1.69E-46 |
| MS.gene055412 | MS.gene78067 | 0.836264 | 1.04E-56 | -1.69E-46 |
| MS.gene055499 | MS.gene78067 | 0.812908 | 3.35E-51 | -1.69E-46 |
| MS.gene055502 | MS.gene78067 | 0.811557 | 6.60E-51 | -1.69E-46 |
| MS.gene055636 | MS.gene78067 | 0.82318 | 1.59E-53 | -1.69E-46 |
| MS.gene055647 | MS.gene78067 | 0.820305 | 7.36E-53 | -1.69E-46 |
| MS.gene055722 | MS.gene78067 | 0.862116 | 6.36E-64 | -1.69E-46 |
| MS.gene05592 | MS.gene78067 | 0.846902 | 1.62E-59 | -1.69E-46 |
| MS.gene055941 | MS.gene78067 | 0.823654 | 1.23E-53 | -1.69E-46 |
| MS.gene056083 | MS.gene78067 | 0.805072 | 1.59E-49 | -1.69E-46 |
| MS.gene056374 | MS.gene78067 | 0.825677 | 4.11E-54 | -1.69E-46 |
| MS.gene056410 | MS.gene78067 | 0.800335 | 1.51E-48 | -1.69E-46 |
| MS.gene056511 | MS.gene78067 | 0.831107 | 2.01E-55 | -1.69E-46 |
| MS.gene056549 | MS.gene78067 | 0.833661 | 4.69E-56 | -1.69E-46 |
| MS.gene056609 | MS.gene78067 | 0.84392 | 1.04E-58 | -1.69E-46 |
| MS.gene056897 | MS.gene78067 | 0.865261 | 6.71E-65 | -1.69E-46 |
| MS.gene057011 | MS.gene78067 | 0.831782 | 1.37E-55 | -1.69E-46 |
| MS.gene057076 | MS.gene78067 | 0.809255 | 2.07E-50 | -1.69E-46 |
| MS.gene057129 | MS.gene78067 | 0.817909 | 2.58E-52 | -1.69E-46 |
| MS.gene057146 | MS.gene78067 | 0.834277 | 3.29E-56 | -1.69E-46 |
| MS.gene057331 | MS.gene78067 | 0.825706 | 4.05E-54 | -1.69E-46 |
| MS.gene057416 | MS.gene78067 | 0.838921 | 2.16E-57 | -1.69E-46 |
| MS.gene057856 | MS.gene78067 | 0.803542 | 3.32E-49 | -1.69E-46 |
| MS.gene057926 | MS.gene78067 | 0.811495 | 6.80E-51 | -1.69E-46 |
| MS.gene058006 | MS.gene78067 | 0.833778 | 4.39E-56 | -1.69E-46 |
| MS.gene058019 | MS.gene78067 | 0.802267 | 6.08E-49 | -1.69E-46 |
| MS.gene058323 | MS.gene78067 | 0.821447 | 4.02E-53 | -1.69E-46 |
| MS.gene058419 | MS.gene78067 | 0.812005 | 5.27E-51 | -1.69E-46 |
| MS.gene058660 | MS.gene78067 | 0.808586 | 2.88E-50 | -1.69E-46 |
| MS.gene058723 | MS.gene78067 | 0.803324 | 3.68E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene78067 | 0.804161 | 2.47E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene78067 | 0.824852 | 6.45E-54 | -1.69E-46 |
| MS.gene059029 | MS.gene78067 | 0.813296 | 2.75E-51 | -1.69E-46 |
| MS.gene05913 | MS.gene78067 | 0.818104 | 2.33E-52 | -1.69E-46 |
| MS.gene059305 | MS.gene78067 | 0.803348 | 3.64E-49 | -1.69E-46 |
| MS.gene05934 | MS.gene78067 | 0.827957 | 1.17E-54 | -1.69E-46 |
| MS.gene059366 | MS.gene78067 | 0.810138 | 1.34E-50 | -1.69E-46 |
| MS.gene059536 | MS.gene78067 | 0.864923 | 8.57E-65 | -1.69E-46 |
| MS.gene059597 | MS.gene78067 | 0.802106 | 6.57E-49 | -1.69E-46 |
| MS.gene059676 | MS.gene78067 | 0.80754 | 4.81E-50 | -1.69E-46 |
| MS.gene059688 | MS.gene78067 | 0.841338 | 5.05E-58 | -1.69E-46 |
| MS.gene059692 | MS.gene78067 | 0.83115 | 1.97E-55 | -1.69E-46 |
| MS.gene059695 | MS.gene78067 | 0.829689 | 4.47E-55 | -1.69E-46 |
| MS.gene059711 | MS.gene78067 | 0.80928 | 2.05E-50 | -1.69E-46 |
| MS.gene059718 | MS.gene78067 | 0.813455 | 2.54E-51 | -1.69E-46 |
| MS.gene059902 | MS.gene78067 | 0.844734 | 6.29E-59 | -1.69E-46 |
| MS.gene059960 | MS.gene78067 | 0.833946 | 3.98E-56 | -1.69E-46 |
| MS.gene060762 | MS.gene78067 | 0.824929 | 6.19E-54 | -1.69E-46 |
| MS.gene060804 | MS.gene78067 | 0.861302 | 1.13E-63 | -1.69E-46 |
| MS.gene060818 | MS.gene78067 | 0.872918 | 2.20E-67 | -1.69E-46 |
| MS.gene060832 | MS.gene78067 | 0.802957 | 4.38E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene78067 | 0.845302 | 4.42E-59 | -1.69E-46 |
| MS.gene06092 | MS.gene78067 | 0.812011 | 5.25E-51 | -1.69E-46 |
| MS.gene061041 | MS.gene78067 | 0.844225 | 8.63E-59 | -1.69E-46 |
| MS.gene061150 | MS.gene78067 | 0.806887 | 6.61E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene78067 | 0.840774 | 7.10E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78067.t1 | MTR_4g035825 | 92.683 | 123 | 9 | 0 | 1 | 123 | 30 | 152 | 3.64e-70 | 209 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78067.t1 | AT5G51545 | 35.616 | 146 | 66 | 4 | 1 | 123 | 1 | 141 | 1.43e-16 | 72.0 |
Find 25 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGAGAGTGGTGTTGAAAA+AGG | 0.238655 | 4.1:+64656354 | MS.gene78067:CDS |
| ATTCTTGCTTGGCTTGGTTT+TGG | 0.242979 | 4.1:+64656381 | MS.gene78067:CDS |
| GGTATTGCTCTTGCTGCTTC+AGG | 0.275480 | 4.1:+64656423 | MS.gene78067:CDS |
| AACAAAACCGGTGTTGGTTT+TGG | 0.305798 | 4.1:+64656201 | MS.gene78067:CDS |
| TGCTCTTGCTGCTTCAGGTC+AGG | 0.356798 | 4.1:+64656428 | MS.gene78067:CDS |
| GAGAAAGAGCTTCTATAATT+CGG | 0.362300 | 4.1:+64656268 | MS.gene78067:CDS |
| TCGGATTTGATTGTGAAGAT+AGG | 0.366284 | 4.1:-64656147 | None:intergenic |
| GGTTTCATTCTTGCTTGGCT+TGG | 0.405039 | 4.1:+64656375 | MS.gene78067:CDS |
| GGTTCGGTTATTCTTGTTGA+GGG | 0.408372 | 4.1:+64656402 | MS.gene78067:CDS |
| TTGCCCTTTCTGTTTCCGTT+TGG | 0.419619 | 4.1:-64656244 | None:intergenic |
| AGAGAATGAGTGATGTAGTT+TGG | 0.434413 | 4.1:-64656124 | None:intergenic |
| AACACCAAACGGAAACAGAA+AGG | 0.495671 | 4.1:+64656240 | MS.gene78067:CDS |
| GATTCAGTTCCCAACAAAAC+CGG | 0.500611 | 4.1:+64656189 | MS.gene78067:CDS |
| ACACCAAACGGAAACAGAAA+GGG | 0.501372 | 4.1:+64656241 | MS.gene78067:CDS |
| TGGTTCGGTTATTCTTGTTG+AGG | 0.511249 | 4.1:+64656401 | MS.gene78067:CDS |
| TTGGTGTTGTTGCCATTCGA+CGG | 0.523797 | 4.1:-64656225 | None:intergenic |
| AATTGTTCCGAAACGAAAGC+CGG | 0.532646 | 4.1:-64656309 | None:intergenic |
| CGAATGGCAACAACACCAAA+CGG | 0.552467 | 4.1:+64656229 | MS.gene78067:CDS |
| GTTCCCAACAAAACCGGTGT+TGG | 0.564166 | 4.1:+64656195 | MS.gene78067:CDS |
| ACTGAATCGGACGGTTCAGA+TGG | 0.575343 | 4.1:-64656174 | None:intergenic |
| GTGATGTAGTTTGGTGGTGA+AGG | 0.593400 | 4.1:-64656115 | None:intergenic |
| GAATGAGTGATGTAGTTTGG+TGG | 0.606738 | 4.1:-64656121 | None:intergenic |
| CGACGGACCAAAACCAACAC+CGG | 0.616034 | 4.1:-64656208 | None:intergenic |
| CAAGAAGTTCAAGAAGAGAG+TGG | 0.626455 | 4.1:+64656342 | MS.gene78067:CDS |
| TTGTTGGGAACTGAATCGGA+CGG | 0.685336 | 4.1:-64656183 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAGGTTTCATTCTTGCT+TGG | + | chr4.1:64656370-64656389 | MS.gene78067:CDS | 30.0% | |
| GAGAAAGAGCTTCTATAATT+CGG | + | chr4.1:64656268-64656287 | MS.gene78067:CDS | 30.0% | |
| AGAGAATGAGTGATGTAGTT+TGG | - | chr4.1:64656127-64656146 | None:intergenic | 35.0% | |
| TCGGATTTGATTGTGAAGAT+AGG | - | chr4.1:64656150-64656169 | None:intergenic | 35.0% | |
| AACACCAAACGGAAACAGAA+AGG | + | chr4.1:64656240-64656259 | MS.gene78067:CDS | 40.0% | |
| AATTGTTCCGAAACGAAAGC+CGG | - | chr4.1:64656312-64656331 | None:intergenic | 40.0% | |
| ACACCAAACGGAAACAGAAA+GGG | + | chr4.1:64656241-64656260 | MS.gene78067:CDS | 40.0% | |
| CAAGAAGTTCAAGAAGAGAG+TGG | + | chr4.1:64656342-64656361 | MS.gene78067:CDS | 40.0% | |
| GAATGAGTGATGTAGTTTGG+TGG | - | chr4.1:64656124-64656143 | None:intergenic | 40.0% | |
| GATTCAGTTCCCAACAAAAC+CGG | + | chr4.1:64656189-64656208 | MS.gene78067:CDS | 40.0% | |
| GCGTAATCCAGTTGAAAAAC+CGG | + | chr4.1:64656290-64656309 | MS.gene78067:CDS | 40.0% | |
| ! | GAAGAGAGTGGTGTTGAAAA+AGG | + | chr4.1:64656354-64656373 | MS.gene78067:CDS | 40.0% |
| ! | GGTTCGGTTATTCTTGTTGA+GGG | + | chr4.1:64656402-64656421 | MS.gene78067:CDS | 40.0% |
| ! | TGAAAAACCGGCTTTCGTTT+CGG | + | chr4.1:64656302-64656321 | MS.gene78067:CDS | 40.0% |
| !! | TGGTTCGGTTATTCTTGTTG+AGG | + | chr4.1:64656401-64656420 | MS.gene78067:CDS | 40.0% |
| !!! | AAAACCAACACCGGTTTTGT+TGG | - | chr4.1:64656202-64656221 | None:intergenic | 40.0% |
| !!! | AAACCAACACCGGTTTTGTT+GGG | - | chr4.1:64656201-64656220 | None:intergenic | 40.0% |
| !!! | AACAAAACCGGTGTTGGTTT+TGG | + | chr4.1:64656201-64656220 | MS.gene78067:CDS | 40.0% |
| !!! | ATTCTTGCTTGGCTTGGTTT+TGG | + | chr4.1:64656381-64656400 | MS.gene78067:CDS | 40.0% |
| !!! | GGTTTTGTTGGGAACTGAAT+CGG | - | chr4.1:64656190-64656209 | None:intergenic | 40.0% |
| CGAATGGCAACAACACCAAA+CGG | + | chr4.1:64656229-64656248 | MS.gene78067:CDS | 45.0% | |
| GGTTTCATTCTTGCTTGGCT+TGG | + | chr4.1:64656375-64656394 | MS.gene78067:CDS | 45.0% | |
| TTGCCCTTTCTGTTTCCGTT+TGG | - | chr4.1:64656247-64656266 | None:intergenic | 45.0% | |
| TTGTTGGGAACTGAATCGGA+CGG | - | chr4.1:64656186-64656205 | None:intergenic | 45.0% | |
| ! | GGACGGTTCAGATGGTTTTT+CGG | - | chr4.1:64656169-64656188 | None:intergenic | 45.0% |
| !! | ACGAAAGCCGGTTTTTCAAC+TGG | - | chr4.1:64656300-64656319 | None:intergenic | 45.0% |
| !! | GTGATGTAGTTTGGTGGTGA+AGG | - | chr4.1:64656118-64656137 | None:intergenic | 45.0% |
| !! | TTGGTGTTGTTGCCATTCGA+CGG | - | chr4.1:64656228-64656247 | None:intergenic | 45.0% |
| !!! | TGCTTGGCTTGGTTTTGGTT+CGG | + | chr4.1:64656386-64656405 | MS.gene78067:CDS | 45.0% |
| ACTGAATCGGACGGTTCAGA+TGG | - | chr4.1:64656177-64656196 | None:intergenic | 50.0% | |
| GTTCCCAACAAAACCGGTGT+TGG | + | chr4.1:64656195-64656214 | MS.gene78067:CDS | 50.0% | |
| ! | GGTATTGCTCTTGCTGCTTC+AGG | + | chr4.1:64656423-64656442 | MS.gene78067:CDS | 50.0% |
| !!! | GTTGGTTTTGGTCCGTCGAA+TGG | + | chr4.1:64656213-64656232 | MS.gene78067:CDS | 50.0% |
| CGACGGACCAAAACCAACAC+CGG | - | chr4.1:64656211-64656230 | None:intergenic | 55.0% | |
| ! | TGCTCTTGCTGCTTCAGGTC+AGG | + | chr4.1:64656428-64656447 | MS.gene78067:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 64656087 | 64656470 | 64656087 | ID=MS.gene78067 |
| chr4.1 | mRNA | 64656087 | 64656470 | 64656087 | ID=MS.gene78067.t1;Parent=MS.gene78067 |
| chr4.1 | exon | 64656087 | 64656470 | 64656087 | ID=MS.gene78067.t1.exon1;Parent=MS.gene78067.t1 |
| chr4.1 | CDS | 64656087 | 64656470 | 64656087 | ID=cds.MS.gene78067.t1;Parent=MS.gene78067.t1 |
| Gene Sequence |
| Protein sequence |
