Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97617.t1 | XP_013448647.1 | 94.8 | 58 | 3 | 0 | 1 | 58 | 144 | 201 | 4.50E-26 | 126.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97617.t1 | B7FGJ5 | 94.8 | 58 | 3 | 0 | 1 | 58 | 107 | 164 | 3.3e-26 | 126.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene97617 | 0.800142 | 1.66E-48 | -1.69E-46 |
| MS.gene049171 | MS.gene97617 | 0.85713 | 2.01E-62 | -1.69E-46 |
| MS.gene049217 | MS.gene97617 | 0.801326 | 9.49E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene97617 | 0.837123 | 6.26E-57 | -1.69E-46 |
| MS.gene049232 | MS.gene97617 | 0.816407 | 5.62E-52 | -1.69E-46 |
| MS.gene049285 | MS.gene97617 | 0.82526 | 5.17E-54 | -1.69E-46 |
| MS.gene049320 | MS.gene97617 | 0.838477 | 2.81E-57 | -1.69E-46 |
| MS.gene049609 | MS.gene97617 | 0.821356 | 4.22E-53 | -1.69E-46 |
| MS.gene049633 | MS.gene97617 | 0.819877 | 9.22E-53 | -1.69E-46 |
| MS.gene049805 | MS.gene97617 | 0.845452 | 4.03E-59 | -1.69E-46 |
| MS.gene05010 | MS.gene97617 | 0.838777 | 2.35E-57 | -1.69E-46 |
| MS.gene050163 | MS.gene97617 | 0.801052 | 1.08E-48 | -1.69E-46 |
| MS.gene050323 | MS.gene97617 | 0.835084 | 2.06E-56 | -1.69E-46 |
| MS.gene050333 | MS.gene97617 | 0.810807 | 9.59E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene97617 | 0.851117 | 1.09E-60 | -1.69E-46 |
| MS.gene050518 | MS.gene97617 | 0.80779 | 4.25E-50 | -1.69E-46 |
| MS.gene050577 | MS.gene97617 | 0.810475 | 1.13E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene97617 | 0.832994 | 6.88E-56 | -1.69E-46 |
| MS.gene050612 | MS.gene97617 | 0.836456 | 9.26E-57 | -1.69E-46 |
| MS.gene050675 | MS.gene97617 | 0.803798 | 2.93E-49 | -1.69E-46 |
| MS.gene050699 | MS.gene97617 | 0.821117 | 4.79E-53 | -1.69E-46 |
| MS.gene050747 | MS.gene97617 | 0.833666 | 4.68E-56 | -1.69E-46 |
| MS.gene050772 | MS.gene97617 | 0.835077 | 2.07E-56 | -1.69E-46 |
| MS.gene05080 | MS.gene97617 | 0.839388 | 1.63E-57 | -1.69E-46 |
| MS.gene05081 | MS.gene97617 | 0.810782 | 9.71E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene97617 | 0.848519 | 5.82E-60 | -1.69E-46 |
| MS.gene050871 | MS.gene97617 | 0.847686 | 9.88E-60 | -1.69E-46 |
| MS.gene050950 | MS.gene97617 | 0.822144 | 2.77E-53 | -1.69E-46 |
| MS.gene050986 | MS.gene97617 | 0.82601 | 3.43E-54 | -1.69E-46 |
| MS.gene05141 | MS.gene97617 | -0.800623 | 1.32E-48 | -1.69E-46 |
| MS.gene051551 | MS.gene97617 | 0.823544 | 1.31E-53 | -1.69E-46 |
| MS.gene051666 | MS.gene97617 | 0.806341 | 8.62E-50 | -1.69E-46 |
| MS.gene051694 | MS.gene97617 | 0.809111 | 2.22E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene97617 | 0.806745 | 7.09E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene97617 | 0.809958 | 1.46E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene97617 | 0.819546 | 1.10E-52 | -1.69E-46 |
| MS.gene052504 | MS.gene97617 | 0.815309 | 9.88E-52 | -1.69E-46 |
| MS.gene052506 | MS.gene97617 | 0.831971 | 1.23E-55 | -1.69E-46 |
| MS.gene052615 | MS.gene97617 | 0.809805 | 1.58E-50 | -1.69E-46 |
| MS.gene052820 | MS.gene97617 | 0.818061 | 2.39E-52 | -1.69E-46 |
| MS.gene052822 | MS.gene97617 | 0.815497 | 8.97E-52 | -1.69E-46 |
| MS.gene052823 | MS.gene97617 | 0.81753 | 3.14E-52 | -1.69E-46 |
| MS.gene052825 | MS.gene97617 | 0.819016 | 1.45E-52 | -1.69E-46 |
| MS.gene052966 | MS.gene97617 | 0.840449 | 8.64E-58 | -1.69E-46 |
| MS.gene053103 | MS.gene97617 | 0.807207 | 5.66E-50 | -1.69E-46 |
| MS.gene053104 | MS.gene97617 | 0.826199 | 3.09E-54 | -1.69E-46 |
| MS.gene053203 | MS.gene97617 | 0.838774 | 2.35E-57 | -1.69E-46 |
| MS.gene053207 | MS.gene97617 | 0.815479 | 9.05E-52 | -1.69E-46 |
| MS.gene053213 | MS.gene97617 | 0.832565 | 8.79E-56 | -1.69E-46 |
| MS.gene053286 | MS.gene97617 | 0.830038 | 3.68E-55 | -1.69E-46 |
| MS.gene053436 | MS.gene97617 | 0.812336 | 4.46E-51 | -1.69E-46 |
| MS.gene053574 | MS.gene97617 | 0.822262 | 2.60E-53 | -1.69E-46 |
| MS.gene053626 | MS.gene97617 | 0.824527 | 7.69E-54 | -1.69E-46 |
| MS.gene053632 | MS.gene97617 | 0.809459 | 1.87E-50 | -1.69E-46 |
| MS.gene053824 | MS.gene97617 | 0.821587 | 3.73E-53 | -1.69E-46 |
| MS.gene053916 | MS.gene97617 | 0.815848 | 7.49E-52 | -1.69E-46 |
| MS.gene054003 | MS.gene97617 | 0.835662 | 1.47E-56 | -1.69E-46 |
| MS.gene054408 | MS.gene97617 | 0.812609 | 3.89E-51 | -1.69E-46 |
| MS.gene054595 | MS.gene97617 | 0.863876 | 1.82E-64 | -1.69E-46 |
| MS.gene054600 | MS.gene97617 | 0.812776 | 3.58E-51 | -1.69E-46 |
| MS.gene054642 | MS.gene97617 | 0.835401 | 1.72E-56 | -1.69E-46 |
| MS.gene05470 | MS.gene97617 | 0.80389 | 2.81E-49 | -1.69E-46 |
| MS.gene054927 | MS.gene97617 | 0.801478 | 8.84E-49 | -1.69E-46 |
| MS.gene054954 | MS.gene97617 | 0.815311 | 9.87E-52 | -1.69E-46 |
| MS.gene055206 | MS.gene97617 | 0.830494 | 2.85E-55 | -1.69E-46 |
| MS.gene055289 | MS.gene97617 | 0.811615 | 6.41E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene97617 | 0.84255 | 2.41E-58 | -1.69E-46 |
| MS.gene055502 | MS.gene97617 | 0.852573 | 4.22E-61 | -1.69E-46 |
| MS.gene055577 | MS.gene97617 | 0.836115 | 1.13E-56 | -1.69E-46 |
| MS.gene055722 | MS.gene97617 | 0.816819 | 4.54E-52 | -1.69E-46 |
| MS.gene055748 | MS.gene97617 | 0.809721 | 1.65E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene97617 | 0.808168 | 3.54E-50 | -1.69E-46 |
| MS.gene05592 | MS.gene97617 | 0.815291 | 9.97E-52 | -1.69E-46 |
| MS.gene055941 | MS.gene97617 | 0.827436 | 1.57E-54 | -1.69E-46 |
| MS.gene056040 | MS.gene97617 | 0.80944 | 1.89E-50 | -1.69E-46 |
| MS.gene056166 | MS.gene97617 | 0.81045 | 1.15E-50 | -1.69E-46 |
| MS.gene056374 | MS.gene97617 | 0.833852 | 4.20E-56 | -1.69E-46 |
| MS.gene056504 | MS.gene97617 | 0.825698 | 4.07E-54 | -1.69E-46 |
| MS.gene056510 | MS.gene97617 | 0.836949 | 6.93E-57 | -1.69E-46 |
| MS.gene056657 | MS.gene97617 | 0.811657 | 6.28E-51 | -1.69E-46 |
| MS.gene056878 | MS.gene97617 | 0.804971 | 1.67E-49 | -1.69E-46 |
| MS.gene057086 | MS.gene97617 | 0.836581 | 8.60E-57 | -1.69E-46 |
| MS.gene057331 | MS.gene97617 | 0.821395 | 4.13E-53 | -1.69E-46 |
| MS.gene057416 | MS.gene97617 | 0.847301 | 1.26E-59 | -1.69E-46 |
| MS.gene057856 | MS.gene97617 | 0.805097 | 1.57E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene97617 | 0.846018 | 2.83E-59 | -1.69E-46 |
| MS.gene058419 | MS.gene97617 | 0.844105 | 9.29E-59 | -1.69E-46 |
| MS.gene058544 | MS.gene97617 | 0.834138 | 3.56E-56 | -1.69E-46 |
| MS.gene058773 | MS.gene97617 | 0.836838 | 7.40E-57 | -1.69E-46 |
| MS.gene058838 | MS.gene97617 | 0.836189 | 1.08E-56 | -1.69E-46 |
| MS.gene059022 | MS.gene97617 | 0.832857 | 7.44E-56 | -1.69E-46 |
| MS.gene059023 | MS.gene97617 | 0.825328 | 4.98E-54 | -1.69E-46 |
| MS.gene059667 | MS.gene97617 | 0.809159 | 2.17E-50 | -1.69E-46 |
| MS.gene059677 | MS.gene97617 | 0.813824 | 2.10E-51 | -1.69E-46 |
| MS.gene059718 | MS.gene97617 | 0.828105 | 1.08E-54 | -1.69E-46 |
| MS.gene059756 | MS.gene97617 | 0.803948 | 2.73E-49 | -1.69E-46 |
| MS.gene059801 | MS.gene97617 | 0.805394 | 1.36E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene97617 | 0.825247 | 5.20E-54 | -1.69E-46 |
| MS.gene060482 | MS.gene97617 | 0.810389 | 1.18E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene97617 | 0.828206 | 1.02E-54 | -1.69E-46 |
| MS.gene06077 | MS.gene97617 | 0.833283 | 5.83E-56 | -1.69E-46 |
| MS.gene06092 | MS.gene97617 | 0.850198 | 1.98E-60 | -1.69E-46 |
| MS.gene060982 | MS.gene97617 | 0.820792 | 5.69E-53 | -1.69E-46 |
| MS.gene061150 | MS.gene97617 | 0.832352 | 9.93E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97617.t1 | MTR_7g056413 | 94.828 | 58 | 3 | 0 | 1 | 58 | 144 | 201 | 1.25e-34 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCCTTATGGGCATGTTTA+TGG | 0.209138 | 7.4:-47433108 | MS.gene97617:CDS |
| CCTTCGCCAGTTGTTCCTTA+TGG | 0.209497 | 7.4:-47433120 | MS.gene97617:CDS |
| CTTCGCCAGTTGTTCCTTAT+GGG | 0.271859 | 7.4:-47433119 | MS.gene97617:CDS |
| CCATCCATAACTATCGTATA+CGG | 0.371058 | 7.4:+47433047 | None:intergenic |
| TGGGTCATCACCACGATTGC+TGG | 0.388653 | 7.4:-47433008 | MS.gene97617:CDS |
| ACATGCCCATAAGGAACAAC+TGG | 0.446939 | 7.4:+47433114 | None:intergenic |
| GCACCATAAACATGCCCATA+AGG | 0.479031 | 7.4:+47433105 | None:intergenic |
| CTTCTTCGCGCCAGCAATCG+TGG | 0.498790 | 7.4:+47432998 | None:intergenic |
| CGATTGCTGGCGCGAAGAAG+AGG | 0.521477 | 7.4:-47432995 | MS.gene97617:CDS |
| CATGTTTATGGTGCTTGTCA+AGG | 0.539807 | 7.4:-47433096 | MS.gene97617:CDS |
| AGGCCCGTATACGATAGTTA+TGG | 0.549421 | 7.4:-47433051 | MS.gene97617:CDS |
| TGGTGATGACCCACGTAACA+AGG | 0.557049 | 7.4:+47433018 | None:intergenic |
| GTTTATGGTGCTTGTCAAGG+TGG | 0.563579 | 7.4:-47433093 | MS.gene97617:CDS |
| TGGCGAAGGATATGCAAGAA+TGG | 0.564765 | 7.4:+47433134 | None:intergenic |
| ATGGAGTGGGCCTTGTTACG+TGG | 0.565179 | 7.4:-47433028 | MS.gene97617:CDS |
| TACGATAGTTATGGATGGAG+TGG | 0.565922 | 7.4:-47433042 | MS.gene97617:CDS |
| ACGATAGTTATGGATGGAGT+GGG | 0.583338 | 7.4:-47433041 | MS.gene97617:CDS |
| GGCCTCCCATAAAACTGTGG+GGG | 0.585436 | 7.4:+47433069 | None:intergenic |
| CATCCATAACTATCGTATAC+GGG | 0.586554 | 7.4:+47433048 | None:intergenic |
| CGGGCCTCCCATAAAACTGT+GGG | 0.590176 | 7.4:+47433067 | None:intergenic |
| TGGAGTGGGCCTTGTTACGT+GGG | 0.611082 | 7.4:-47433027 | MS.gene97617:CDS |
| GCCTCCCATAAAACTGTGGG+GGG | 0.618515 | 7.4:+47433070 | None:intergenic |
| CCATAAGGAACAACTGGCGA+AGG | 0.625985 | 7.4:+47433120 | None:intergenic |
| CCGTATACGATAGTTATGGA+TGG | 0.632117 | 7.4:-47433047 | MS.gene97617:CDS |
| GGATATGCAAGAATGGACAT+TGG | 0.650120 | 7.4:+47433141 | None:intergenic |
| ACGGGCCTCCCATAAAACTG+TGG | 0.672573 | 7.4:+47433066 | None:intergenic |
| GGGCCTCCCATAAAACTGTG+GGG | 0.723372 | 7.4:+47433068 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CATCCATAACTATCGTATAC+GGG | + | chr7.4:47433079-47433098 | None:intergenic | 35.0% | |
| CCATCCATAACTATCGTATA+CGG | + | chr7.4:47433080-47433099 | None:intergenic | 35.0% | |
| ACGATAGTTATGGATGGAGT+GGG | - | chr7.4:47433083-47433102 | MS.gene97617:CDS | 40.0% | |
| CATGTTTATGGTGCTTGTCA+AGG | - | chr7.4:47433028-47433047 | MS.gene97617:CDS | 40.0% | |
| CCGTATACGATAGTTATGGA+TGG | - | chr7.4:47433077-47433096 | MS.gene97617:CDS | 40.0% | |
| GTTCCTTATGGGCATGTTTA+TGG | - | chr7.4:47433016-47433035 | MS.gene97617:CDS | 40.0% | |
| TACGATAGTTATGGATGGAG+TGG | - | chr7.4:47433082-47433101 | MS.gene97617:CDS | 40.0% | |
| ACATGCCCATAAGGAACAAC+TGG | + | chr7.4:47433013-47433032 | None:intergenic | 45.0% | |
| AGGCCCGTATACGATAGTTA+TGG | - | chr7.4:47433073-47433092 | MS.gene97617:CDS | 45.0% | |
| CTTCGCCAGTTGTTCCTTAT+GGG | - | chr7.4:47433005-47433024 | MS.gene97617:CDS | 45.0% | |
| GCACCATAAACATGCCCATA+AGG | + | chr7.4:47433022-47433041 | None:intergenic | 45.0% | |
| GTTTATGGTGCTTGTCAAGG+TGG | - | chr7.4:47433031-47433050 | MS.gene97617:CDS | 45.0% | |
| TGGCGAAGGATATGCAAGAA+TGG | + | chr7.4:47432993-47433012 | None:intergenic | 45.0% | |
| CCATAAGGAACAACTGGCGA+AGG | + | chr7.4:47433007-47433026 | None:intergenic | 50.0% | |
| CCTTCGCCAGTTGTTCCTTA+TGG | - | chr7.4:47433004-47433023 | MS.gene97617:CDS | 50.0% | |
| ! | TGGTGATGACCCACGTAACA+AGG | + | chr7.4:47433109-47433128 | None:intergenic | 50.0% |
| ACGGGCCTCCCATAAAACTG+TGG | + | chr7.4:47433061-47433080 | None:intergenic | 55.0% | |
| CGGGCCTCCCATAAAACTGT+GGG | + | chr7.4:47433060-47433079 | None:intergenic | 55.0% | |
| GCCTCCCATAAAACTGTGGG+GGG | + | chr7.4:47433057-47433076 | None:intergenic | 55.0% | |
| GGCCTCCCATAAAACTGTGG+GGG | + | chr7.4:47433058-47433077 | None:intergenic | 55.0% | |
| GGGCCTCCCATAAAACTGTG+GGG | + | chr7.4:47433059-47433078 | None:intergenic | 55.0% | |
| TGGGTCATCACCACGATTGC+TGG | - | chr7.4:47433116-47433135 | MS.gene97617:CDS | 55.0% | |
| !! | ATGGAGTGGGCCTTGTTACG+TGG | - | chr7.4:47433096-47433115 | MS.gene97617:CDS | 55.0% |
| !! | GTGGCCCCCCACAGTTTTAT+GGG | - | chr7.4:47433050-47433069 | MS.gene97617:CDS | 55.0% |
| !! | TGGAGTGGGCCTTGTTACGT+GGG | - | chr7.4:47433097-47433116 | MS.gene97617:CDS | 55.0% |
| CGATTGCTGGCGCGAAGAAG+AGG | - | chr7.4:47433129-47433148 | MS.gene97617:CDS | 60.0% | |
| CTTCTTCGCGCCAGCAATCG+TGG | + | chr7.4:47433129-47433148 | None:intergenic | 60.0% | |
| ! | GGTGGCCCCCCACAGTTTTA+TGG | - | chr7.4:47433049-47433068 | MS.gene97617:CDS | 60.0% |
| !! | GCCCCCCACAGTTTTATGGG+AGG | - | chr7.4:47433053-47433072 | MS.gene97617:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 47432986 | 47433160 | 47432986 | ID=MS.gene97617 |
| chr7.4 | mRNA | 47432986 | 47433160 | 47432986 | ID=MS.gene97617.t1;Parent=MS.gene97617 |
| chr7.4 | exon | 47432986 | 47433160 | 47432986 | ID=MS.gene97617.t1.exon1;Parent=MS.gene97617.t1 |
| chr7.4 | CDS | 47432986 | 47433160 | 47432986 | ID=cds.MS.gene97617.t1;Parent=MS.gene97617.t1 |
| Gene Sequence |
| Protein sequence |
