AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024494.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024494.01.T01 MTR_5g012120 82.203 118 5 2 1 117 1 103 1.29e-62 192
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024494.01.T01 AT1G04590 46.053 76 23 3 44 118 122 180 3.44e-13 64.7
MsG0580024494.01.T01 AT1G04590 46.053 76 23 3 44 118 122 180 3.56e-13 64.7

Find 31 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTGTTGTGAGAAGAAATT+TGG 0.218146 5:-5545798 None:intergenic
GAAATACCAAAGCATTATAT+TGG 0.265891 5:+5545749 MsG0580024494.01.T01:CDS
GATAGTAAGGAGGCTATTTA+TGG 0.272982 5:+5546169 MsG0580024494.01.T01:CDS
CTCTTGATGCATGGGTTGCT+TGG 0.290402 5:+5546194 MsG0580024494.01.T01:CDS
TATCACGAAGCTGCATCATT+AGG 0.312015 5:-5546135 None:intergenic
AAATACCAAAGCATTATATT+GGG 0.356702 5:+5545750 MsG0580024494.01.T01:CDS
AATGATGCAGCTTCGTGATA+TGG 0.356783 5:+5546138 MsG0580024494.01.T01:CDS
TCTTGATGCATGGGTTGCTT+GGG 0.383776 5:+5546195 MsG0580024494.01.T01:CDS
CTTCGAAATCTTGCTAGAAA+AGG 0.405557 5:-5545275 None:intergenic
ATCACGAAGCTGCATCATTA+GGG 0.405973 5:-5546134 None:intergenic
TTCTCCCCAATATAATGCTT+TGG 0.412939 5:-5545755 None:intergenic
ATATTTCTCAATGAACCAAT+TGG 0.419484 5:-5546228 None:intergenic
TTTATGGTGCTCTTGATGCA+TGG 0.435289 5:+5546185 MsG0580024494.01.T01:CDS
TTATGGTGCTCTTGATGCAT+GGG 0.444864 5:+5546186 MsG0580024494.01.T01:CDS
AGAGAGAACAAGTGAATGAA+GGG 0.445044 5:-5545207 None:intergenic
TTCTCTCCACATTTCACAGC+AGG 0.446461 5:+5545246 MsG0580024494.01.T01:five_prime_UTR
AAGAGAGAACAAGTGAATGA+AGG 0.452083 5:-5545208 None:intergenic
TGTGGAGAGAATAAAAGTTT+CGG 0.454466 5:-5545234 None:intergenic
TTGGGAGCAAAATTTCCAAT+TGG 0.483107 5:+5546213 MsG0580024494.01.T01:CDS
CTACTAGCTTTCTGTTCAGA+AGG 0.486635 5:-5545725 None:intergenic
TATGGATGATACCGATAGTA+AGG 0.509177 5:+5546156 MsG0580024494.01.T01:CDS
TGTCTGCGAATATGATGGTT+GGG 0.523771 5:-5545687 None:intergenic
TTGTCTGCGAATATGATGGT+TGG 0.577007 5:-5545688 None:intergenic
ATAAATAGCCTCCTTACTAT+CGG 0.582438 5:-5546167 None:intergenic
TCTAGCAAGATTTCGAAGCA+AGG 0.586523 5:+5545280 MsG0580024494.01.T01:five_prime_UTR
AAATCGAGGCGATCTCCACA+TGG 0.619578 5:-5545514 None:intergenic
ATCGATCCTGCTGTGAAATG+TGG 0.619764 5:-5545252 None:intergenic
AATACCAAAGCATTATATTG+GGG 0.621135 5:+5545751 MsG0580024494.01.T01:CDS
GGATGATACCGATAGTAAGG+AGG 0.675030 5:+5546159 MsG0580024494.01.T01:CDS
CAAAACAATCAACAACCATG+TGG 0.714494 5:+5545499 MsG0580024494.01.T01:exon
CTTACGAGATACGAAAATCG+AGG 0.725219 5:-5545528 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GGGGGGGGGGGGGGGGGGGG+GGG + Chr5:5545855-5545874 MsG0580024494.01.T01:intron 100.0%
!! AAATTCATGAATTGAAATGA+GGG - Chr5:5545345-5545364 None:intergenic 20.0%
!!! AAATACCAAAGCATTATATT+GGG + Chr5:5545750-5545769 MsG0580024494.01.T01:CDS 20.0%
!!! TGTTATCCTAGTTTTTTTTT+GGG + Chr5:5545835-5545854 MsG0580024494.01.T01:intron 20.0%
!!! TTATATTGATAGTTTGAGTA+TGG + Chr5:5546074-5546093 MsG0580024494.01.T01:intron 20.0%
! CAAATTCATGAATTGAAATG+AGG - Chr5:5545346-5545365 None:intergenic 25.0%
!! AATACCAAAGCATTATATTG+GGG + Chr5:5545751-5545770 MsG0580024494.01.T01:CDS 25.0%
!! GAAATACCAAAGCATTATAT+TGG + Chr5:5545749-5545768 MsG0580024494.01.T01:CDS 25.0%
!!! ATTTGATTATTTCTGTGTTC+TGG + Chr5:5545367-5545386 MsG0580024494.01.T01:intron 25.0%
!!! GTGTATTTTTTCTGTTTTTC+AGG + Chr5:5545607-5545626 MsG0580024494.01.T01:intron 25.0%
!!! GTGTTATCCTAGTTTTTTTT+TGG + Chr5:5545834-5545853 MsG0580024494.01.T01:intron 25.0%
!!! GTTATCCTAGTTTTTTTTTG+GGG + Chr5:5545836-5545855 MsG0580024494.01.T01:intron 25.0%
!!! TTATCCTAGTTTTTTTTTGG+GGG + Chr5:5545837-5545856 MsG0580024494.01.T01:intron 25.0%
ATAAATAGCCTCCTTACTAT+CGG - Chr5:5546170-5546189 None:intergenic 30.0%
ATAAGCTTCAACAACGTTAA+TGG - Chr5:5545572-5545591 None:intergenic 30.0%
ATACAATTGTTGTGTGTTCT+GGG + Chr5:5545396-5545415 MsG0580024494.01.T01:intron 30.0%
TACTGTTGTGAGAAGAAATT+TGG - Chr5:5545801-5545820 None:intergenic 30.0%
TATACAATTGTTGTGTGTTC+TGG + Chr5:5545395-5545414 MsG0580024494.01.T01:intron 30.0%
TGTGGAGAGAATAAAAGTTT+CGG - Chr5:5545237-5545256 None:intergenic 30.0%
!!! TATCCTAGTTTTTTTTTGGG+GGG + Chr5:5545838-5545857 MsG0580024494.01.T01:intron 30.0%
AAGAGAGAACAAGTGAATGA+AGG - Chr5:5545211-5545230 None:intergenic 35.0%
AGAGAGAACAAGTGAATGAA+GGG - Chr5:5545210-5545229 None:intergenic 35.0%
ATAGTTTGAGTATGGTGACA+GGG + Chr5:5546082-5546101 MsG0580024494.01.T01:intron 35.0%
CAAAACAATCAACAACCATG+TGG + Chr5:5545499-5545518 MsG0580024494.01.T01:exon 35.0%
CTTCGAAATCTTGCTAGAAA+AGG - Chr5:5545278-5545297 None:intergenic 35.0%
GATAGTAAGGAGGCTATTTA+TGG + Chr5:5546169-5546188 MsG0580024494.01.T01:CDS 35.0%
TATGGATGATACCGATAGTA+AGG + Chr5:5546156-5546175 MsG0580024494.01.T01:CDS 35.0%
TTCTCCCCAATATAATGCTT+TGG - Chr5:5545758-5545777 None:intergenic 35.0%
TTGGGAGCAAAATTTCCAAT+TGG + Chr5:5546213-5546232 MsG0580024494.01.T01:CDS 35.0%
! GATTTTGTCTGCGAATATGA+TGG - Chr5:5545695-5545714 None:intergenic 35.0%
!!! ATCCTAGTTTTTTTTTGGGG+GGG + Chr5:5545839-5545858 MsG0580024494.01.T01:intron 35.0%
!!! TGACAGGGGTTTCAATTTTT+TGG + Chr5:5546097-5546116 MsG0580024494.01.T01:intron 35.0%
AATGATGCAGCTTCGTGATA+TGG + Chr5:5546138-5546157 MsG0580024494.01.T01:CDS 40.0%
ATCACGAAGCTGCATCATTA+GGG - Chr5:5546137-5546156 None:intergenic 40.0%
CTTACGAGATACGAAAATCG+AGG - Chr5:5545531-5545550 None:intergenic 40.0%
GATAGTTTGAGTATGGTGAC+AGG + Chr5:5546081-5546100 MsG0580024494.01.T01:intron 40.0%
TAGTTTGAGTATGGTGACAG+GGG + Chr5:5546083-5546102 MsG0580024494.01.T01:intron 40.0%
TATCACGAAGCTGCATCATT+AGG - Chr5:5546138-5546157 None:intergenic 40.0%
TCTAGCAAGATTTCGAAGCA+AGG + Chr5:5545280-5545299 MsG0580024494.01.T01:five_prime_UTR 40.0%
TGTCTGCGAATATGATGGTT+GGG - Chr5:5545690-5545709 None:intergenic 40.0%
TTATGGTGCTCTTGATGCAT+GGG + Chr5:5546186-5546205 MsG0580024494.01.T01:CDS 40.0%
TTGTCTGCGAATATGATGGT+TGG - Chr5:5545691-5545710 None:intergenic 40.0%
TTTATGGTGCTCTTGATGCA+TGG + Chr5:5546185-5546204 MsG0580024494.01.T01:CDS 40.0%
!! CTACTAGCTTTCTGTTCAGA+AGG - Chr5:5545728-5545747 None:intergenic 40.0%
!!! TCCTAGTTTTTTTTTGGGGG+GGG + Chr5:5545840-5545859 MsG0580024494.01.T01:intron 40.0%
AATCCTTGGACCAAATACCG+AGG - Chr5:5545888-5545907 None:intergenic 45.0%
AGCGAGAGCAAAGTTCTGTA+TGG + Chr5:5545949-5545968 MsG0580024494.01.T01:intron 45.0%
ATCGATCCTGCTGTGAAATG+TGG - Chr5:5545255-5545274 None:intergenic 45.0%
CTAGTGAGTAACACACTCCT+AGG + Chr5:5545995-5546014 MsG0580024494.01.T01:intron 45.0%
GGATGATACCGATAGTAAGG+AGG + Chr5:5546159-5546178 MsG0580024494.01.T01:CDS 45.0%
TCCAAGGATTCACTAATCCG+AGG + Chr5:5545898-5545917 MsG0580024494.01.T01:intron 45.0%
TTCAATTGAGCTCCCGCTAT+GGG - Chr5:5545930-5545949 None:intergenic 45.0%
TTCTCTCCACATTTCACAGC+AGG + Chr5:5545246-5545265 MsG0580024494.01.T01:five_prime_UTR 45.0%
! CTAGGAGTGTGTTACTCACT+AGG - Chr5:5545997-5546016 None:intergenic 45.0%
! TCTTGATGCATGGGTTGCTT+GGG + Chr5:5546195-5546214 MsG0580024494.01.T01:CDS 45.0%
!! TCCTCGGATTAGTGAATCCT+TGG - Chr5:5545902-5545921 None:intergenic 45.0%
!!! CCTAGTTTTTTTTTGGGGGG+GGG + Chr5:5545841-5545860 MsG0580024494.01.T01:intron 45.0%
!!! CTAGTTTTTTTTTGGGGGGG+GGG + Chr5:5545842-5545861 MsG0580024494.01.T01:intron 45.0%
!!! TAGTTTTTTTTTGGGGGGGG+GGG + Chr5:5545843-5545862 MsG0580024494.01.T01:intron 45.0%
AAATCGAGGCGATCTCCACA+TGG - Chr5:5545517-5545536 None:intergenic 50.0%
CACTAGGTCTCACGTTGTGT+TGG - Chr5:5545981-5546000 None:intergenic 50.0%
CCCCCCCCCAAAAAAAAACT+AGG - Chr5:5545844-5545863 None:intergenic 50.0%
CTTCAATTGAGCTCCCGCTA+TGG - Chr5:5545931-5545950 None:intergenic 50.0%
CTTCACCATGCCAACCACTT+GGG + Chr5:5546033-5546052 MsG0580024494.01.T01:intron 50.0%
TTCACCATGCCAACCACTTG+GGG + Chr5:5546034-5546053 MsG0580024494.01.T01:intron 50.0%
! CTCTTGATGCATGGGTTGCT+TGG + Chr5:5546194-5546213 MsG0580024494.01.T01:CDS 50.0%
!!! AGTTTTTTTTTGGGGGGGGG+GGG + Chr5:5545844-5545863 MsG0580024494.01.T01:intron 50.0%
CCAAGTGGTTGGCATGGTGA+AGG - Chr5:5546035-5546054 None:intergenic 55.0%
CCTTCACCATGCCAACCACT+TGG + Chr5:5546032-5546051 MsG0580024494.01.T01:intron 55.0%
TCACCATGCCAACCACTTGG+GGG + Chr5:5546035-5546054 MsG0580024494.01.T01:intron 55.0%
TCCGAGGACCAATCCCATAG+CGG + Chr5:5545914-5545933 MsG0580024494.01.T01:intron 55.0%
TTGAGCTCCCGCTATGGGAT+TGG - Chr5:5545925-5545944 None:intergenic 55.0%
!!! GTTTTTTTTTGGGGGGGGGG+GGG + Chr5:5545845-5545864 MsG0580024494.01.T01:intron 55.0%
!!! TTTTTTTTTGGGGGGGGGGG+GGG + Chr5:5545846-5545865 MsG0580024494.01.T01:intron 55.0%
AGGCTGGAGAGTCGAGACCT+AGG - Chr5:5546015-5546034 None:intergenic 60.0%
CAAACCCCTTCACCCCCAAG+TGG - Chr5:5546050-5546069 None:intergenic 60.0%
CCAACCACTTGGGGGTGAAG+GGG + Chr5:5546043-5546062 MsG0580024494.01.T01:intron 60.0%
CCGAGGACCAATCCCATAGC+GGG + Chr5:5545915-5545934 MsG0580024494.01.T01:intron 60.0%
GCCAACCACTTGGGGGTGAA+GGG + Chr5:5546042-5546061 MsG0580024494.01.T01:intron 60.0%
GGGCCTCGGTATTTGGTCCA+AGG + Chr5:5545882-5545901 MsG0580024494.01.T01:intron 60.0%
GTGGTTGGCATGGTGAAGGC+TGG - Chr5:5546031-5546050 None:intergenic 60.0%
TCACCCCCAAGTGGTTGGCA+TGG - Chr5:5546041-5546060 None:intergenic 60.0%
TGCCAACCACTTGGGGGTGA+AGG + Chr5:5546041-5546060 MsG0580024494.01.T01:intron 60.0%
!! CCCGCTATGGGATTGGTCCT+CGG - Chr5:5545918-5545937 None:intergenic 60.0%
!!! TTTTTTTTGGGGGGGGGGGG+GGG + Chr5:5545847-5545866 MsG0580024494.01.T01:intron 60.0%
CCCCTTCACCCCCAAGTGGT+TGG - Chr5:5546046-5546065 None:intergenic 65.0%
!!! TTTTTTTGGGGGGGGGGGGG+GGG + Chr5:5545848-5545867 MsG0580024494.01.T01:intron 65.0%
!!! TTTTTTGGGGGGGGGGGGGG+GGG + Chr5:5545849-5545868 MsG0580024494.01.T01:intron 70.0%
GGGGGGGGGGCCTCGGTATT+TGG + Chr5:5545875-5545894 MsG0580024494.01.T01:intron 75.0%
!! TTTTTGGGGGGGGGGGGGGG+GGG + Chr5:5545850-5545869 MsG0580024494.01.T01:intron 75.0%
!!! TTTTGGGGGGGGGGGGGGGG+GGG + Chr5:5545851-5545870 MsG0580024494.01.T01:intron 80.0%
! TTTGGGGGGGGGGGGGGGGG+GGG + Chr5:5545852-5545871 MsG0580024494.01.T01:intron 85.0%
! TTGGGGGGGGGGGGGGGGGG+GGG + Chr5:5545853-5545872 MsG0580024494.01.T01:intron 90.0%
! GGGGGGGGGGGGGGGGGCCT+CGG + Chr5:5545868-5545887 MsG0580024494.01.T01:intron 95.0%
! TGGGGGGGGGGGGGGGGGGG+GGG + Chr5:5545854-5545873 MsG0580024494.01.T01:intron 95.0%
Chromosome Type Strat End Strand Name
Chr5 gene 5545187 5546243 5545187 ID=MsG0580024494.01;Name=MsG0580024494.01
Chr5 mRNA 5545187 5546243 5545187 ID=MsG0580024494.01.T01;Parent=MsG0580024494.01;Name=MsG0580024494.01.T01;_AED=0.47;_eAED=0.50;_QI=199|0.66|0.75|1|0|0|4|0|119
Chr5 exon 5545187 5545301 5545187 ID=MsG0580024494.01.T01:exon:19088;Parent=MsG0580024494.01.T01
Chr5 exon 5545432 5545545 5545432 ID=MsG0580024494.01.T01:exon:19089;Parent=MsG0580024494.01.T01
Chr5 exon 5545629 5545817 5545629 ID=MsG0580024494.01.T01:exon:19090;Parent=MsG0580024494.01.T01
Chr5 exon 5546103 5546243 5546103 ID=MsG0580024494.01.T01:exon:19091;Parent=MsG0580024494.01.T01
Chr5 five_prime_UTR 5545187 5545301 5545187 ID=MsG0580024494.01.T01:five_prime_utr;Parent=MsG0580024494.01.T01
Chr5 five_prime_UTR 5545432 5545515 5545432 ID=MsG0580024494.01.T01:five_prime_utr;Parent=MsG0580024494.01.T01
Chr5 CDS 5545516 5545545 5545516 ID=MsG0580024494.01.T01:cds;Parent=MsG0580024494.01.T01
Chr5 CDS 5545629 5545817 5545629 ID=MsG0580024494.01.T01:cds;Parent=MsG0580024494.01.T01
Chr5 CDS 5546103 5546243 5546103 ID=MsG0580024494.01.T01:cds;Parent=MsG0580024494.01.T01
Gene Sequence

>MsG0580024494.01.T01

ATGTGGAGATCGCCTCGATTTTCGTATCTCGTTACTAGATTGAGTCAAGTTAACATAAATGTCAATCGATGTTACAGTCAAATGTTGTCCCAACCATCATATTCGCAGACAAAATCTGAATCTATTCCTTCTGAACAGAAAGCTAGTAGAGAAATACCAAAGCATTATATTGGGGAGAATGTGTCTCGAAAAGATAGAACCAAATTTCTTCTCACAACAGGGTTTCAATTTTTTGGTGTTTTGATTAAATCCCTAATGATGCAGCTTCGTGATATGGATGATACCGATAGTAAGGAGGCTATTTATGGTGCTCTTGATGCATGGGTTGCTTGGGAGCAAAATTTCCAATTGGTTCATTGA

Protein sequence

>MsG0580024494.01.T01

MWRSPRFSYLVTRLSQVNINVNRCYSQMLSQPSYSQTKSESIPSEQKASREIPKHYIGENVSRKDRTKFLLTTGFQFFGVLIKSLMMQLRDMDDTDSKEAIYGALDAWVAWEQNFQLVH*