Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022198.01.T01 | XP_003607854.1 | 82.979 | 235 | 16 | 2 | 1 | 211 | 150 | 384 | 2.37E-134 | 395 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022198.01.T01 | Q9MA87 | 57.025 | 242 | 69 | 6 | 1 | 208 | 156 | 396 | 3.69E-85 | 263 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022198.01.T01 | G7JIR1 | 82.979 | 235 | 16 | 2 | 1 | 211 | 150 | 384 | 1.13e-134 | 395 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048178.01 | MsG0480022198.01 | 0.818326 | 2.077929e-52 | 1.550613e-49 |
| MsG0080048205.01 | MsG0480022198.01 | 0.811987 | 5.316928e-51 | 3.339567e-48 |
| MsG0080048809.01 | MsG0480022198.01 | -0.844864 | 5.806523e-59 | 9.500444e-56 |
| MsG0080048982.01 | MsG0480022198.01 | 0.835234 | 1.889196e-56 | 2.296300e-53 |
| MsG0180000271.01 | MsG0480022198.01 | 0.819483 | 1.134435e-52 | 8.741051e-50 |
| MsG0180000673.01 | MsG0480022198.01 | 0.800613 | 1.327868e-48 | 6.210302e-46 |
| MsG0180000680.01 | MsG0480022198.01 | 0.803743 | 3.012867e-49 | 1.525972e-46 |
| MsG0180000797.01 | MsG0480022198.01 | 0.800007 | 1.763299e-48 | 8.121078e-46 |
| MsG0180000855.01 | MsG0480022198.01 | 0.802721 | 4.904610e-49 | 2.419990e-46 |
| MsG0180001312.01 | MsG0480022198.01 | 0.848122 | 7.492708e-60 | 1.360880e-56 |
| MsG0180001663.01 | MsG0480022198.01 | 0.821517 | 3.870498e-53 | 3.155746e-50 |
| MsG0180001904.01 | MsG0480022198.01 | 0.824219 | 9.088776e-54 | 8.000083e-51 |
| MsG0180001922.01 | MsG0480022198.01 | 0.810562 | 1.083874e-50 | 6.554992e-48 |
| MsG0180002071.01 | MsG0480022198.01 | 0.806260 | 8.967437e-50 | 4.845498e-47 |
| MsG0180002955.01 | MsG0480022198.01 | 0.807954 | 3.926586e-50 | 2.216695e-47 |
| MsG0180004085.01 | MsG0480022198.01 | 0.855785 | 4.995837e-62 | 1.166883e-58 |
| MsG0180004599.01 | MsG0480022198.01 | 0.836296 | 1.016926e-56 | 1.276294e-53 |
| MsG0180004600.01 | MsG0480022198.01 | 0.852222 | 5.318944e-61 | 1.103806e-57 |
| MsG0180004602.01 | MsG0480022198.01 | 0.809683 | 1.676377e-50 | 9.907031e-48 |
| MsG0180005050.01 | MsG0480022198.01 | 0.800199 | 1.611914e-48 | 7.459939e-46 |
| MsG0180005160.01 | MsG0480022198.01 | 0.864686 | 1.017103e-64 | 3.221478e-61 |
| MsG0180005304.01 | MsG0480022198.01 | 0.803342 | 3.649186e-49 | 1.829210e-46 |
| MsG0180005354.01 | MsG0480022198.01 | 0.827113 | 1.872071e-54 | 1.790157e-51 |
| MsG0180005398.01 | MsG0480022198.01 | 0.809120 | 2.214027e-50 | 1.288967e-47 |
| MsG0180005505.01 | MsG0480022198.01 | 0.807733 | 4.376199e-50 | 2.456298e-47 |
| MsG0180005743.01 | MsG0480022198.01 | 0.820637 | 6.174309e-53 | 4.911941e-50 |
| MsG0180005960.01 | MsG0480022198.01 | 0.827551 | 1.469399e-54 | 1.423189e-51 |
| MsG0180006016.01 | MsG0480022198.01 | 0.829851 | 4.084446e-55 | 4.231745e-52 |
| MsG0180006138.01 | MsG0480022198.01 | 0.802991 | 4.314409e-49 | 2.143270e-46 |
| MsG0280006356.01 | MsG0480022198.01 | 0.826666 | 2.393497e-54 | 2.259320e-51 |
| MsG0280006440.01 | MsG0480022198.01 | 0.811448 | 6.966816e-51 | 4.313753e-48 |
| MsG0280010614.01 | MsG0480022198.01 | 0.800513 | 1.391645e-48 | 6.492109e-46 |
| MsG0280010784.01 | MsG0480022198.01 | 0.851135 | 1.080623e-60 | 2.165883e-57 |
| MsG0280010901.01 | MsG0480022198.01 | 0.801880 | 7.308913e-49 | 3.529872e-46 |
| MsG0280011131.01 | MsG0480022198.01 | 0.827109 | 1.876018e-54 | 1.793699e-51 |
| MsG0280011191.01 | MsG0480022198.01 | 0.807365 | 5.237245e-50 | 2.911514e-47 |
| MsG0280011235.01 | MsG0480022198.01 | 0.842159 | 3.065808e-58 | 4.607489e-55 |
| MsG0280011376.01 | MsG0480022198.01 | 0.808714 | 2.704311e-50 | 1.557341e-47 |
| MsG0280011410.01 | MsG0480022198.01 | 0.840510 | 8.327621e-58 | 1.188896e-54 |
| MsG0380011537.01 | MsG0480022198.01 | 0.830689 | 2.548989e-55 | 2.705585e-52 |
| MsG0380011754.01 | MsG0480022198.01 | 0.822901 | 1.848015e-53 | 1.567320e-50 |
| MsG0380011870.01 | MsG0480022198.01 | 0.802209 | 6.252494e-49 | 3.044903e-46 |
| MsG0380011895.01 | MsG0480022198.01 | 0.837902 | 3.951720e-57 | 5.206927e-54 |
| MsG0380012020.01 | MsG0480022198.01 | 0.823431 | 1.390102e-53 | 1.196634e-50 |
| MsG0380012279.01 | MsG0480022198.01 | -0.809088 | 2.249363e-50 | 1.308385e-47 |
| MsG0380012458.01 | MsG0480022198.01 | 0.803148 | 4.002048e-49 | 1.996222e-46 |
| MsG0380014551.01 | MsG0480022198.01 | -0.823114 | 1.648549e-53 | 1.406616e-50 |
| MsG0380014561.01 | MsG0480022198.01 | 0.804034 | 2.621966e-49 | 1.337971e-46 |
| MsG0380014573.01 | MsG0480022198.01 | 0.812492 | 4.124988e-51 | 2.626589e-48 |
| MsG0380014770.01 | MsG0480022198.01 | 0.832438 | 9.450555e-56 | 1.056330e-52 |
| MsG0480018473.01 | MsG0480022198.01 | 0.814594 | 1.422759e-51 | 9.587012e-49 |
| MsG0480018635.01 | MsG0480022198.01 | 0.822842 | 1.907882e-53 | 1.615376e-50 |
| MsG0480019335.01 | MsG0480022198.01 | 0.802252 | 6.128647e-49 | 2.987953e-46 |
| MsG0480019434.01 | MsG0480022198.01 | 0.823581 | 1.282353e-53 | 1.108631e-50 |
| MsG0480020416.01 | MsG0480022198.01 | 0.804930 | 1.705167e-49 | 8.904930e-47 |
| MsG0480020964.01 | MsG0480022198.01 | 0.806060 | 9.880482e-50 | 5.311571e-47 |
| MsG0480021021.01 | MsG0480022198.01 | 0.804338 | 2.265968e-49 | 1.165344e-46 |
| MsG0480021240.01 | MsG0480022198.01 | 0.806135 | 9.530008e-50 | 5.132823e-47 |
| MsG0480021426.01 | MsG0480022198.01 | 0.810748 | 9.880204e-51 | 6.005227e-48 |
| MsG0480021695.01 | MsG0480022198.01 | 0.804174 | 2.451752e-49 | 1.255577e-46 |
| MsG0480022108.01 | MsG0480022198.01 | 0.805112 | 1.561943e-49 | 8.194650e-47 |
| MsG0480022198.01 | MsG0480022480.01 | 0.801142 | 1.035235e-48 | 4.907287e-46 |
| MsG0480022198.01 | MsG0480022597.01 | 0.815372 | 9.561181e-52 | 6.580113e-49 |
| MsG0480022198.01 | MsG0480022933.01 | 0.832983 | 6.922618e-56 | 7.864878e-53 |
| MsG0480022198.01 | MsG0480023224.01 | 0.830693 | 2.543501e-55 | 2.700058e-52 |
| MsG0480022198.01 | MsG0480023248.01 | 0.830670 | 2.576608e-55 | 2.733334e-52 |
| MsG0480022198.01 | MsG0480023270.01 | 0.817763 | 2.786308e-52 | 2.047324e-49 |
| MsG0480022198.01 | MsG0480023427.01 | 0.804641 | 1.959314e-49 | 1.015531e-46 |
| MsG0480022198.01 | MsG0480023718.01 | 0.802232 | 6.186270e-49 | 3.014453e-46 |
| MsG0480022198.01 | MsG0480023937.01 | 0.819392 | 1.190005e-52 | 9.145150e-50 |
| MsG0480022198.01 | MsG0480024014.01 | 0.812247 | 4.667914e-51 | 2.952818e-48 |
| MsG0480022198.01 | MsG0580024114.01 | 0.845891 | 3.060324e-59 | 5.175778e-56 |
| MsG0480022198.01 | MsG0580024160.01 | 0.804291 | 2.317779e-49 | 1.190561e-46 |
| MsG0480022198.01 | MsG0580024433.01 | 0.810377 | 1.188143e-50 | 7.150223e-48 |
| MsG0480022198.01 | MsG0580024472.01 | 0.803752 | 3.000050e-49 | 1.519834e-46 |
| MsG0480022198.01 | MsG0580024581.01 | 0.814343 | 1.617260e-51 | 1.082398e-48 |
| MsG0480022198.01 | MsG0580025008.01 | 0.804757 | 1.853388e-49 | 9.635541e-47 |
| MsG0480022198.01 | MsG0580025009.01 | 0.821107 | 4.814586e-53 | 3.881166e-50 |
| MsG0480022198.01 | MsG0580025026.01 | 0.826357 | 2.835853e-54 | 2.653465e-51 |
| MsG0480022198.01 | MsG0580025328.01 | 0.804836 | 1.783800e-49 | 9.292978e-47 |
| MsG0480022198.01 | MsG0580025749.01 | 0.800629 | 1.317935e-48 | 6.166472e-46 |
| MsG0480022198.01 | MsG0580025758.01 | 0.817772 | 2.772974e-52 | 2.038055e-49 |
| MsG0480022198.01 | MsG0580025907.01 | 0.834727 | 2.535429e-56 | 3.034475e-53 |
| MsG0480022198.01 | MsG0580026023.01 | 0.808738 | 2.672620e-50 | 1.540042e-47 |
| MsG0480022198.01 | MsG0580027129.01 | 0.818912 | 1.529745e-52 | 1.160275e-49 |
| MsG0480022198.01 | MsG0580027228.01 | 0.804600 | 1.998611e-49 | 1.034790e-46 |
| MsG0480022198.01 | MsG0580028289.01 | 0.814585 | 1.429370e-51 | 9.629125e-49 |
| MsG0480022198.01 | MsG0680030656.01 | 0.843515 | 1.336615e-58 | 2.096279e-55 |
| MsG0480022198.01 | MsG0680030685.01 | 0.809781 | 1.596547e-50 | 9.460071e-48 |
| MsG0480022198.01 | MsG0680030839.01 | 0.800509 | 1.393892e-48 | 6.501999e-46 |
| MsG0480022198.01 | MsG0680031114.01 | 0.807081 | 6.015719e-50 | 3.319611e-47 |
| MsG0480022198.01 | MsG0680031334.01 | 0.812300 | 4.543949e-51 | 2.878462e-48 |
| MsG0480022198.01 | MsG0680031377.01 | -0.832975 | 6.952982e-56 | 7.897422e-53 |
| MsG0480022198.01 | MsG0680032058.01 | 0.819764 | 9.787137e-53 | 7.600539e-50 |
| MsG0480022198.01 | MsG0680032380.01 | 0.839019 | 2.035616e-57 | 2.775073e-54 |
| MsG0480022198.01 | MsG0680034067.01 | 0.813391 | 2.620895e-51 | 1.709463e-48 |
| MsG0480022198.01 | MsG0680034219.01 | 0.806563 | 7.739971e-50 | 4.214520e-47 |
| MsG0480022198.01 | MsG0680034450.01 | 0.821948 | 3.077033e-53 | 2.539684e-50 |
| MsG0480022198.01 | MsG0680034914.01 | 0.810613 | 1.056402e-50 | 6.397757e-48 |
| MsG0480022198.01 | MsG0680035844.01 | 0.827729 | 1.331873e-54 | 1.296711e-51 |
| MsG0480022198.01 | MsG0780035958.01 | 0.818191 | 2.229262e-52 | 1.657360e-49 |
| MsG0480022198.01 | MsG0780036289.01 | 0.831573 | 1.545890e-55 | 1.684474e-52 |
| MsG0480022198.01 | MsG0780036950.01 | 0.801149 | 1.031569e-48 | 4.890865e-46 |
| MsG0480022198.01 | MsG0780037512.01 | 0.802434 | 5.619732e-49 | 2.752641e-46 |
| MsG0480022198.01 | MsG0780038092.01 | 0.823298 | 1.493745e-53 | 1.281050e-50 |
| MsG0480022198.01 | MsG0780038231.01 | 0.811159 | 8.047393e-51 | 4.944734e-48 |
| MsG0480022198.01 | MsG0780038636.01 | 0.813133 | 2.986971e-51 | 1.934358e-48 |
| MsG0480022198.01 | MsG0780038644.01 | 0.828019 | 1.134383e-54 | 1.113929e-51 |
| MsG0480022198.01 | MsG0780038827.01 | 0.802526 | 5.381708e-49 | 2.642026e-46 |
| MsG0480022198.01 | MsG0780038833.01 | 0.824708 | 6.972191e-54 | 6.224809e-51 |
| MsG0480022198.01 | MsG0780038911.01 | 0.806482 | 8.052699e-50 | 4.375692e-47 |
| MsG0480022198.01 | MsG0780039143.01 | 0.805973 | 1.030400e-49 | 5.526996e-47 |
| MsG0480022198.01 | MsG0780039252.01 | 0.803271 | 3.774313e-49 | 1.888550e-46 |
| MsG0480022198.01 | MsG0780039405.01 | 0.800096 | 1.691539e-48 | 7.808181e-46 |
| MsG0480022198.01 | MsG0780039515.01 | -0.802953 | 4.392572e-49 | 2.180049e-46 |
| MsG0480022198.01 | MsG0780039777.01 | 0.803748 | 3.006501e-49 | 1.522917e-46 |
| MsG0480022198.01 | MsG0780040679.01 | 0.820439 | 6.855944e-53 | 5.424947e-50 |
| MsG0480022198.01 | MsG0780040712.01 | 0.811890 | 5.581495e-51 | 3.496422e-48 |
| MsG0480022198.01 | MsG0780041376.01 | 0.801844 | 7.433141e-49 | 3.586546e-46 |
| MsG0480022198.01 | MsG0880042055.01 | 0.815846 | 7.499964e-52 | 5.228661e-49 |
| MsG0480022198.01 | MsG0880042743.01 | 0.827641 | 1.398720e-54 | 1.358197e-51 |
| MsG0480022198.01 | MsG0880043172.01 | 0.806131 | 9.544731e-50 | 5.140296e-47 |
| MsG0480022198.01 | MsG0880043591.01 | 0.814249 | 1.696504e-51 | 1.132508e-48 |
| MsG0480022198.01 | MsG0880043804.01 | 0.804296 | 2.312828e-49 | 1.188154e-46 |
| MsG0480022198.01 | MsG0880044346.01 | 0.801811 | 7.548302e-49 | 3.639052e-46 |
| MsG0480022198.01 | MsG0880044573.01 | 0.846620 | 1.936874e-59 | 3.353496e-56 |
| MsG0480022198.01 | MsG0880044638.01 | 0.802180 | 6.338979e-49 | 3.084744e-46 |
| MsG0480022198.01 | MsG0880045106.01 | 0.811142 | 8.117810e-51 | 4.985807e-48 |
| MsG0480022198.01 | MsG0880045290.01 | 0.808112 | 3.633584e-50 | 2.059868e-47 |
| MsG0480022198.01 | MsG0880046209.01 | 0.811730 | 6.049110e-51 | 3.773506e-48 |
| MsG0480022198.01 | MsG0880046244.01 | 0.846940 | 1.583616e-59 | 2.770079e-56 |
| MsG0480022198.01 | MsG0880046733.01 | 0.816012 | 6.886609e-52 | 4.823294e-49 |
| MsG0480022198.01 | MsG0880047019.01 | 0.812018 | 5.235953e-51 | 3.291416e-48 |
| MsG0480022198.01 | MsG0880047143.01 | 0.810818 | 9.538641e-51 | 5.808566e-48 |
| MsG0480022198.01 | MsG0880047391.01 | 0.803390 | 3.566539e-49 | 1.790030e-46 |
| MsG0480022198.01 | MsG0880047425.01 | 0.829181 | 5.942774e-55 | 6.036961e-52 |
| MsG0480022198.01 | MsG0880047590.01 | 0.802220 | 6.219974e-49 | 3.029936e-46 |
| MsG0480022198.01 | MsG0880047594.01 | 0.807325 | 5.342511e-50 | 2.967006e-47 |
| MsG0380015614.01 | MsG0480022198.01 | 0.806710 | 7.205667e-50 | 3.938366e-47 |
| MsG0380015931.01 | MsG0480022198.01 | 0.804650 | 1.950624e-49 | 1.011275e-46 |
| MsG0380016182.01 | MsG0480022198.01 | 0.840117 | 1.054946e-57 | 1.487900e-54 |
| MsG0380016581.01 | MsG0480022198.01 | 0.832207 | 1.078251e-55 | 1.196900e-52 |
| MsG0380016624.01 | MsG0480022198.01 | 0.838796 | 2.323930e-57 | 3.146879e-54 |
| MsG0380017210.01 | MsG0480022198.01 | 0.806324 | 8.691866e-50 | 4.704058e-47 |
| MsG0380017220.01 | MsG0480022198.01 | 0.806955 | 6.396969e-50 | 3.518532e-47 |
| MsG0380017233.01 | MsG0480022198.01 | 0.809985 | 1.443051e-50 | 8.596410e-48 |
| MsG0380017286.01 | MsG0480022198.01 | 0.830809 | 2.381901e-55 | 2.537169e-52 |
| MsG0380017551.01 | MsG0480022198.01 | -0.854381 | 1.278136e-61 | 2.848985e-58 |
| MsG0380017941.01 | MsG0480022198.01 | 0.800812 | 1.209101e-48 | 5.683349e-46 |
| MsG0480018318.01 | MsG0480022198.01 | 0.811508 | 6.759091e-51 | 4.191825e-48 |
| MsG0480018321.01 | MsG0480022198.01 | 0.816453 | 5.486784e-52 | 3.889262e-49 |
| MsG0280006462.01 | MsG0480022198.01 | 0.821440 | 4.034198e-53 | 3.282126e-50 |
| MsG0280006935.01 | MsG0480022198.01 | 0.819295 | 1.251857e-52 | 9.594560e-50 |
| MsG0280007311.01 | MsG0480022198.01 | 0.811055 | 8.478016e-51 | 5.195380e-48 |
| MsG0280007610.01 | MsG0480022198.01 | 0.800472 | 1.418633e-48 | 6.611008e-46 |
| MsG0280007826.01 | MsG0480022198.01 | 0.815608 | 8.473422e-52 | 5.869671e-49 |
| MsG0280007864.01 | MsG0480022198.01 | -0.814140 | 1.792529e-51 | 1.193107e-48 |
| MsG0280008295.01 | MsG0480022198.01 | 0.812958 | 3.263043e-51 | 2.103178e-48 |
| MsG0280008907.01 | MsG0480022198.01 | 0.808520 | 2.975444e-50 | 1.704937e-47 |
| MsG0280008970.01 | MsG0480022198.01 | 0.809206 | 2.121654e-50 | 1.238067e-47 |
| MsG0280009024.01 | MsG0480022198.01 | 0.842375 | 2.686476e-58 | 4.065537e-55 |
| MsG0280009754.01 | MsG0480022198.01 | 0.808077 | 3.697798e-50 | 2.094292e-47 |
| MsG0280010389.01 | MsG0480022198.01 | 0.812773 | 3.580751e-51 | 2.296784e-48 |
| MsG0280010416.01 | MsG0480022198.01 | 0.832775 | 7.796017e-56 | 8.802100e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022198.01.T01 | MTR_4g083590 | 82.979 | 235 | 16 | 2 | 1 | 211 | 150 | 384 | 2.87e-138 | 395 |
| MsG0480022198.01.T01 | MTR_2g028900 | 63.333 | 240 | 59 | 4 | 1 | 211 | 154 | 393 | 4.77e-100 | 298 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022198.01.T01 | AT3G05320 | 57.025 | 242 | 69 | 6 | 1 | 208 | 156 | 396 | 3.76e-86 | 263 |
| MsG0480022198.01.T01 | AT3G05320 | 57.025 | 242 | 69 | 6 | 1 | 208 | 156 | 396 | 3.76e-86 | 263 |
| MsG0480022198.01.T01 | AT3G05320 | 57.025 | 242 | 69 | 6 | 1 | 208 | 156 | 396 | 3.76e-86 | 263 |
Find 40 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACACAAAAGATGATCAAAT+TGG | 0.268660 | 4:-69566298 | MsG0480022198.01.T01:CDS |
| CAAGACTCTGCAACATTTGC+TGG | 0.312126 | 4:-69565721 | MsG0480022198.01.T01:CDS |
| GTGGACAGTTCAAAGGGATT+TGG | 0.314132 | 4:-69566926 | MsG0480022198.01.T01:CDS |
| CTTTCTTGTGGCATGATCAT+TGG | 0.319430 | 4:-69566834 | MsG0480022198.01.T01:CDS |
| AACTTTGCATAGTCCTTAAC+AGG | 0.328734 | 4:+69566811 | None:intergenic |
| ATTGCAGACTGAATTAAATA+AGG | 0.349906 | 4:+69566402 | None:intergenic |
| ACTTCAAAATTCTTCAGTAT+TGG | 0.401889 | 4:-69566493 | MsG0480022198.01.T01:CDS |
| TAGTAGCAAGAATGAGATAA+TGG | 0.408374 | 4:-69566586 | MsG0480022198.01.T01:CDS |
| TTGAGGGCTGATATCTTTGT+TGG | 0.409638 | 4:-69566363 | MsG0480022198.01.T01:CDS |
| ACTGCAAGATAAAGAACAAC+TGG | 0.413135 | 4:+69566528 | None:intergenic |
| ATTCTTCAGTATTGGAAGGT+TGG | 0.422323 | 4:-69566485 | MsG0480022198.01.T01:CDS |
| ACCAAGTTTCTTCTCTCATA+AGG | 0.423974 | 4:+69566447 | None:intergenic |
| ATGAATTACCTTTATCATCA+TGG | 0.426306 | 4:+69566712 | None:intergenic |
| ACATGAGAGTGGAAATTGAT+TGG | 0.428798 | 4:-69566659 | MsG0480022198.01.T01:CDS |
| ATTGAGGTGATTAAGATTGA+TGG | 0.430449 | 4:-69566865 | MsG0480022198.01.T01:CDS |
| GTTGGTAGCCATGATGATAA+AGG | 0.445451 | 4:-69566720 | MsG0480022198.01.T01:CDS |
| GCATGATCATTGGCCTGTTA+AGG | 0.447996 | 4:-69566824 | MsG0480022198.01.T01:CDS |
| GTATTGGAAGGTTGGAAAGA+AGG | 0.448989 | 4:-69566477 | MsG0480022198.01.T01:CDS |
| ATGAGATAATGGAAAGAGTA+GGG | 0.459739 | 4:-69566575 | MsG0480022198.01.T01:CDS |
| ACACAAAAGATGATCAAATT+GGG | 0.466064 | 4:-69566297 | MsG0480022198.01.T01:CDS |
| GCCTCTATGATTAGAAAAGT+TGG | 0.474344 | 4:-69566738 | MsG0480022198.01.T01:intron |
| TCACATGTGATGTAGAGAAT+TGG | 0.475344 | 4:-69565751 | MsG0480022198.01.T01:intron |
| CAATGATCATGCCACAAGAA+AGG | 0.489335 | 4:+69566835 | None:intergenic |
| ACCTTATGAGAGAAGAAACT+TGG | 0.490608 | 4:-69566448 | MsG0480022198.01.T01:CDS |
| AATGAGATAATGGAAAGAGT+AGG | 0.495512 | 4:-69566576 | MsG0480022198.01.T01:CDS |
| TTGAGGTGATTAAGATTGAT+GGG | 0.506436 | 4:-69566864 | MsG0480022198.01.T01:CDS |
| AATGATCATGCCACAAGAAA+GGG | 0.506447 | 4:+69566836 | None:intergenic |
| TTGATTATGAAGTGTGTTTG+AGG | 0.513101 | 4:-69566380 | MsG0480022198.01.T01:CDS |
| CAAAATTCTTCAGTATTGGA+AGG | 0.520833 | 4:-69566489 | MsG0480022198.01.T01:CDS |
| GGTTGAATGCAGAAAGGAAG+TGG | 0.524410 | 4:-69566955 | None:intergenic |
| TGATTATGAAGTGTGTTTGA+GGG | 0.535787 | 4:-69566379 | MsG0480022198.01.T01:CDS |
| GTAGAAAGTGGACAGTTCAA+AGG | 0.550514 | 4:-69566933 | MsG0480022198.01.T01:CDS |
| GAAAGAGTAGGGAACATTGA+AGG | 0.553082 | 4:-69566564 | MsG0480022198.01.T01:CDS |
| GCATTATTTCAAATCTCACT+CGG | 0.564028 | 4:+69565670 | None:intergenic |
| TAGAAAGTGGACAGTTCAAA+GGG | 0.575946 | 4:-69566932 | MsG0480022198.01.T01:CDS |
| AGAAGTGGTTGAATGCAGAA+AGG | 0.615056 | 4:-69566961 | None:intergenic |
| AGAAAGGAAGTGGTAGAAAG+TGG | 0.623667 | 4:-69566945 | MsG0480022198.01.T01:CDS |
| CTCATGTGAACAGCAACATG+AGG | 0.653852 | 4:+69566675 | None:intergenic |
| TGTTGCTGTTCACATGAGAG+TGG | 0.664107 | 4:-69566670 | MsG0480022198.01.T01:CDS |
| TTGGGTGTGAATGAAATGTG+TGG | 0.683523 | 4:-69566279 | MsG0480022198.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAATTTTTTTTTATTAA+CGG | - | Chr4:69566597-69566616 | MsG0480022198.01.T01:CDS | 0.0% |
| !! | AAGGAAAAAAAAAATACATA+AGG | + | Chr4:69566840-69566859 | None:intergenic | 15.0% |
| !! | TTCTTTGCATATAATATAAA+AGG | - | Chr4:69566389-69566408 | MsG0480022198.01.T01:CDS | 15.0% |
| !!! | TTTTATATTATATGCAAAGA+AGG | + | Chr4:69566390-69566409 | None:intergenic | 15.0% |
| !! | AATATAAAAGGAAAATCGAA+TGG | - | Chr4:69566401-69566420 | MsG0480022198.01.T01:CDS | 20.0% |
| !! | CATACTTTAAATTTATGTAG+AGG | - | Chr4:69566694-69566713 | MsG0480022198.01.T01:CDS | 20.0% |
| !! | GTAATGTAATAAGATAATAC+AGG | + | Chr4:69566670-69566689 | None:intergenic | 20.0% |
| !!! | TGTATTTTTTTTTTCCTTAC+TGG | - | Chr4:69566842-69566861 | MsG0480022198.01.T01:CDS | 20.0% |
| ! | AACACAAAAGATGATCAAAT+TGG | - | Chr4:69566329-69566348 | MsG0480022198.01.T01:CDS | 25.0% |
| ! | ACACAAAAGATGATCAAATT+GGG | - | Chr4:69566330-69566349 | MsG0480022198.01.T01:CDS | 25.0% |
| ! | ACTTCAAAATTCTTCAGTAT+TGG | - | Chr4:69566134-69566153 | MsG0480022198.01.T01:intron | 25.0% |
| ! | ATGAATTACCTTTATCATCA+TGG | + | Chr4:69565918-69565937 | None:intergenic | 25.0% |
| ! | ATTGCAGACTGAATTAAATA+AGG | + | Chr4:69566228-69566247 | None:intergenic | 25.0% |
| ! | CAGTTGAAACAGTATAAAAA+TGG | - | Chr4:69565726-69565745 | MsG0480022198.01.T01:CDS | 25.0% |
| ! | TGATGTCGTAATAATGATAT+AGG | + | Chr4:69566549-69566568 | None:intergenic | 25.0% |
| ATATTTGTAACCCATCTCAT+AGG | + | Chr4:69566428-69566447 | None:intergenic | 30.0% | |
| ATTGAGGTGATTAAGATTGA+TGG | - | Chr4:69565762-69565781 | MsG0480022198.01.T01:intron | 30.0% | |
| GATGATTCATTGCAAAAAAC+TGG | - | Chr4:69565990-69566009 | MsG0480022198.01.T01:intron | 30.0% | |
| TAGTAGCAAGAATGAGATAA+TGG | - | Chr4:69566041-69566060 | MsG0480022198.01.T01:intron | 30.0% | |
| TGATTATGAAGTGTGTTTGA+GGG | - | Chr4:69566248-69566267 | MsG0480022198.01.T01:intron | 30.0% | |
| TTGAGGTGATTAAGATTGAT+GGG | - | Chr4:69565763-69565782 | MsG0480022198.01.T01:intron | 30.0% | |
| TTGATTATGAAGTGTGTTTG+AGG | - | Chr4:69566247-69566266 | MsG0480022198.01.T01:intron | 30.0% | |
| ! | ACCAACTTTTCTAATCATAG+AGG | + | Chr4:69565893-69565912 | None:intergenic | 30.0% |
| ! | CAAAATTCTTCAGTATTGGA+AGG | - | Chr4:69566138-69566157 | MsG0480022198.01.T01:intron | 30.0% |
| ! | CTTCACAACAAAAGCATAAT+CGG | - | Chr4:69566520-69566539 | MsG0480022198.01.T01:CDS | 30.0% |
| !! | AATGAGATAATGGAAAGAGT+AGG | - | Chr4:69566051-69566070 | MsG0480022198.01.T01:intron | 30.0% |
| !! | ATGAGATAATGGAAAGAGTA+GGG | - | Chr4:69566052-69566071 | MsG0480022198.01.T01:intron | 30.0% |
| !! | TGGTTCTTTTGATGATATTG+AGG | - | Chr4:69565746-69565765 | MsG0480022198.01.T01:intron | 30.0% |
| AACTTTGCATAGTCCTTAAC+AGG | + | Chr4:69565819-69565838 | None:intergenic | 35.0% | |
| AATGATCATGCCACAAGAAA+GGG | + | Chr4:69565794-69565813 | None:intergenic | 35.0% | |
| ACATGAGAGTGGAAATTGAT+TGG | - | Chr4:69565968-69565987 | MsG0480022198.01.T01:intron | 35.0% | |
| ACCAAGTTTCTTCTCTCATA+AGG | + | Chr4:69566183-69566202 | None:intergenic | 35.0% | |
| ACCTTATGAGAGAAGAAACT+TGG | - | Chr4:69566179-69566198 | MsG0480022198.01.T01:intron | 35.0% | |
| ACTGCAAGATAAAGAACAAC+TGG | + | Chr4:69566102-69566121 | None:intergenic | 35.0% | |
| ATACAGGCTCTATCAAACTA+TGG | + | Chr4:69566654-69566673 | None:intergenic | 35.0% | |
| GAATACAGTTGTTGAAACTG+AGG | - | Chr4:69566728-69566747 | MsG0480022198.01.T01:intron | 35.0% | |
| GACATTCTTCATGCTTCAAA+AGG | - | Chr4:69566797-69566816 | MsG0480022198.01.T01:intron | 35.0% | |
| GCCTCTATGATTAGAAAAGT+TGG | - | Chr4:69565889-69565908 | MsG0480022198.01.T01:intron | 35.0% | |
| TAGAAAGTGGACAGTTCAAA+GGG | - | Chr4:69565695-69565714 | MsG0480022198.01.T01:CDS | 35.0% | |
| TCACATGTGATGTAGAGAAT+TGG | - | Chr4:69566876-69566895 | MsG0480022198.01.T01:CDS | 35.0% | |
| TCTTGAGTAATTCATCAACC+AGG | + | Chr4:69565856-69565875 | None:intergenic | 35.0% | |
| TGAGACAAAATCAACCAGTA+AGG | + | Chr4:69566859-69566878 | None:intergenic | 35.0% | |
| ! | ATTCTTCAGTATTGGAAGGT+TGG | - | Chr4:69566142-69566161 | MsG0480022198.01.T01:intron | 35.0% |
| ! | CTAGCTTTCAACAAGAAATG+TGG | + | Chr4:69566489-69566508 | None:intergenic | 35.0% |
| AATCGAATGGTCCTATGAGA+TGG | - | Chr4:69566414-69566433 | MsG0480022198.01.T01:CDS | 40.0% | |
| AGAAAGGAAGTGGTAGAAAG+TGG | - | Chr4:69565682-69565701 | MsG0480022198.01.T01:CDS | 40.0% | |
| ATCGAATGGTCCTATGAGAT+GGG | - | Chr4:69566415-69566434 | MsG0480022198.01.T01:CDS | 40.0% | |
| ATGGGAAAAACCCTTTCTTG+TGG | - | Chr4:69565781-69565800 | MsG0480022198.01.T01:intron | 40.0% | |
| CAATGATCATGCCACAAGAA+AGG | + | Chr4:69565795-69565814 | None:intergenic | 40.0% | |
| CTTTCTTGTGGCATGATCAT+TGG | - | Chr4:69565793-69565812 | MsG0480022198.01.T01:intron | 40.0% | |
| GAAAGAGTAGGGAACATTGA+AGG | - | Chr4:69566063-69566082 | MsG0480022198.01.T01:intron | 40.0% | |
| GTAGAAAGTGGACAGTTCAA+AGG | - | Chr4:69565694-69565713 | MsG0480022198.01.T01:CDS | 40.0% | |
| GTATTGGAAGGTTGGAAAGA+AGG | - | Chr4:69566150-69566169 | MsG0480022198.01.T01:intron | 40.0% | |
| TGTGTGGAAGTGATGTAAGA+TGG | - | Chr4:69566364-69566383 | MsG0480022198.01.T01:CDS | 40.0% | |
| TTGAGGGCTGATATCTTTGT+TGG | - | Chr4:69566264-69566283 | MsG0480022198.01.T01:intron | 40.0% | |
| TTGGGTGTGAATGAAATGTG+TGG | - | Chr4:69566348-69566367 | MsG0480022198.01.T01:CDS | 40.0% | |
| !! | GTTGGTAGCCATGATGATAA+AGG | - | Chr4:69565907-69565926 | MsG0480022198.01.T01:intron | 40.0% |
| !!! | AGTTTTTGAGTGCTTAGTCC+TGG | - | Chr4:69565835-69565854 | MsG0480022198.01.T01:intron | 40.0% |
| CAAGACTCTGCAACATTTGC+TGG | - | Chr4:69566906-69566925 | MsG0480022198.01.T01:CDS | 45.0% | |
| CTCATGTGAACAGCAACATG+AGG | + | Chr4:69565955-69565974 | None:intergenic | 45.0% | |
| GTGCATAGCTGATAGCATGA+AGG | + | Chr4:69566463-69566482 | None:intergenic | 45.0% | |
| GTGGACAGTTCAAAGGGATT+TGG | - | Chr4:69565701-69565720 | MsG0480022198.01.T01:CDS | 45.0% | |
| ! | GCATGATCATTGGCCTGTTA+AGG | - | Chr4:69565803-69565822 | MsG0480022198.01.T01:intron | 45.0% |
| ! | TGTTGCTGTTCACATGAGAG+TGG | - | Chr4:69565957-69565976 | MsG0480022198.01.T01:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 69565678 | 69566971 | 69565678 | ID=MsG0480022198.01;Name=MsG0480022198.01 |
| Chr4 | mRNA | 69565678 | 69566971 | 69565678 | ID=MsG0480022198.01.T01;Parent=MsG0480022198.01;Name=MsG0480022198.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|0.66|1|1|3|0|237 |
| Chr4 | exon | 69566814 | 69566971 | 69566814 | ID=MsG0480022198.01.T01:exon:5339;Parent=MsG0480022198.01.T01 |
| Chr4 | exon | 69566272 | 69566746 | 69566272 | ID=MsG0480022198.01.T01:exon:5338;Parent=MsG0480022198.01.T01 |
| Chr4 | exon | 69565678 | 69565758 | 69565678 | ID=MsG0480022198.01.T01:exon:5337;Parent=MsG0480022198.01.T01 |
| Chr4 | CDS | 69566814 | 69566971 | 69566814 | ID=MsG0480022198.01.T01:cds;Parent=MsG0480022198.01.T01 |
| Chr4 | CDS | 69566272 | 69566746 | 69566272 | ID=MsG0480022198.01.T01:cds;Parent=MsG0480022198.01.T01 |
| Chr4 | CDS | 69565678 | 69565758 | 69565678 | ID=MsG0480022198.01.T01:cds;Parent=MsG0480022198.01.T01 |
| Gene Sequence |
| Protein sequence |
