AlfalfaGEDB | Msa0633860

M. sativa cultivar zhongmu-4 / Msa0633860

KEGG: -
GO: GO:0000003 GO:0000902 GO:0000904 GO:0001101 GO:0003006 GO:0003674 GO:0005215 GO:0005319 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006649 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0007275 GO:0008150 GO:0009414 GO:0009415 GO:0009505 GO:0009506 GO:0009507 GO:0009534 GO:0009536 GO:0009579 GO:0009628 GO:0009653 GO:0009791 GO:0009826 GO:0009888 GO:0009889 GO:0009987 GO:0010026 GO:0010035 GO:0010154 GO:0010556 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016049 GO:0019222 GO:0022412 GO:0022414 GO:0030054 GO:0030154 GO:0030312 GO:0031410 GO:0031976 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032504 GO:0032989 GO:0033036 GO:0040007 GO:0042221 GO:0042335 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0048316 GO:0048468 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0060255 GO:0060560 GO:0061024 GO:0061458 GO:0065007 GO:0071702 GO:0071840 GO:0071944 GO:0090376 GO:0090378 GO:0090558 GO:0090627 GO:0097708 GO:0098791 GO:1901700 GO:1901957
PFAM: PF00234.25

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0633860	KEH29195.1	85.047	107	16	0	1	107	58	164	5.21e-58	187

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0633860	sp\|A0AT31\|NLTP5_LENCU	72.973	111	30	0	3	113	4	114	4.65e-52	163

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003470	Msa0633860	0.800060	1.720799e-48	-8.615850e-47
Msa0008330	Msa0633860	0.819094	1.390766e-52	-8.615850e-47
Msa0017120	Msa0633860	0.817196	3.737794e-52	-8.615850e-47
Msa0057260	Msa0633860	0.825406	4.770521e-54	-8.615850e-47
Msa0059960	Msa0633860	-0.806305	8.773415e-50	-8.615850e-47
Msa0065390	Msa0633860	0.828445	8.954362e-55	-8.615850e-47
Msa0072820	Msa0633860	0.804749	1.860672e-49	-8.615850e-47
Msa0089210	Msa0633860	0.804900	1.730266e-49	-8.615850e-47
Msa0094420	Msa0633860	0.805753	1.146201e-49	-8.615850e-47
Msa0103780	Msa0633860	0.829154	6.033093e-55	-8.615850e-47
Msa0110710	Msa0633860	0.829474	5.044819e-55	-8.615850e-47
Msa0607280	Msa0633860	0.816170	6.348942e-52	-8.615850e-47
Msa0614230	Msa0633860	0.816533	5.265844e-52	-8.615850e-47
Msa0617120	Msa0633860	0.812197	4.785935e-51	-8.615850e-47
Msa0633860	Msa0634270	0.855000	8.456940e-62	-8.615850e-47
Msa0633860	Msa0634420	0.906556	1.307328e-80	-8.615850e-47
Msa0633860	Msa0638710	0.825790	3.868423e-54	-8.615850e-47
Msa0633860	Msa0640880	0.841349	5.013661e-58	-8.615850e-47
Msa0633860	Msa0652360	0.801006	1.103669e-48	-8.615850e-47
Msa0633860	Msa0660820	0.825941	3.562092e-54	-8.615850e-47
Msa0633860	Msa0663650	0.816468	5.445740e-52	-8.615850e-47
Msa0633860	Msa0702070	0.837239	5.845278e-57	-8.615850e-47
Msa0633860	Msa0702120	0.830913	2.246803e-55	-8.615850e-47
Msa0633860	Msa0720270	0.811302	7.494306e-51	-8.615850e-47
Msa0633860	Msa0738870	0.807762	4.315006e-50	-8.615850e-47
Msa0633860	Msa0738970	0.804808	1.808367e-49	-8.615850e-47
Msa0633860	Msa0773080	0.804734	1.873475e-49	-8.615850e-47
Msa0633860	Msa0838660	0.833772	4.401728e-56	-8.615850e-47
Msa0633860	Msa0859390	0.808603	2.856204e-50	-8.615850e-47
Msa0633860	Msa0894530	0.800041	1.735953e-48	-8.615850e-47
Msa0633860	Msa0931480	0.860794	1.610541e-63	-8.615850e-47
Msa0633860	Msa0955310	0.814152	1.782237e-51	-8.615850e-47
Msa0633860	Msa1004500	0.811866	5.651511e-51	-8.615850e-47
Msa0633860	Msa1022310	0.818984	1.473613e-52	-8.615850e-47
Msa0633860	Msa1027790	0.806368	8.508950e-50	-8.615850e-47
Msa0633860	Msa1035090	0.804786	1.827314e-49	-8.615850e-47
Msa0633860	Msa1061180	0.816118	6.519215e-52	-8.615850e-47
Msa0633860	Msa1103620	0.807660	4.535982e-50	-8.615850e-47
Msa0633860	Msa1106560	0.802388	5.745662e-49	-8.615850e-47
Msa0633860	Msa1106580	0.810182	1.308711e-50	-8.615850e-47
Msa0633860	Msa1112610	0.800080	1.704695e-48	-8.615850e-47
Msa0633860	Msa1116010	0.850864	1.288571e-60	-8.615850e-47
Msa0633860	Msa1134400	0.816101	6.577796e-52	-8.615850e-47
Msa0633860	Msa1162470	0.819920	9.015586e-53	-8.615850e-47
Msa0633860	Msa1180410	0.810004	1.429699e-50	-8.615850e-47
Msa0633860	Msa1199210	0.806306	8.769655e-50	-8.615850e-47
Msa0633860	Msa1208510	0.857976	1.130928e-62	-8.615850e-47
Msa0633860	Msa1219250	0.807460	5.000060e-50	-8.615850e-47
Msa0633860	Msa1220740	0.825469	4.608204e-54	-8.615850e-47
Msa0633860	Msa1221260	0.804523	2.073916e-49	-8.615850e-47
Msa0633860	Msa1229640	0.849480	3.147506e-60	-8.615850e-47
Msa0633860	Msa1232010	0.809005	2.343055e-50	-8.615850e-47
Msa0633860	Msa1232860	0.806784	6.953101e-50	-8.615850e-47
Msa0633860	Msa1237750	0.814546	1.458252e-51	-8.615850e-47
Msa0633860	Msa1238730	0.802257	6.113763e-49	-8.615850e-47
Msa0633860	Msa1244180	0.801497	8.755685e-49	-8.615850e-47
Msa0633860	Msa1254210	0.812779	3.571100e-51	-8.615850e-47
Msa0633860	Msa1258810	0.846256	2.434330e-59	-8.615850e-47
Msa0633860	Msa1263590	0.826546	2.557054e-54	-8.615850e-47
Msa0633860	Msa1263670	0.828159	1.049543e-54	-8.615850e-47
Msa0633860	Msa1272340	0.847125	1.409674e-59	-8.615850e-47
Msa0633860	Msa1274740	0.803627	3.185075e-49	-8.615850e-47
Msa0633860	Msa1275290	0.802187	6.319246e-49	-8.615850e-47
Msa0633860	Msa1284030	0.833070	6.586805e-56	-8.615850e-47
Msa0633860	Msa1293530	0.803228	3.853660e-49	-8.615850e-47
Msa0633860	Msa1302150	0.808459	3.065259e-50	-8.615850e-47
Msa0633860	Msa1310250	0.841834	3.735223e-58	-8.615850e-47
Msa0633860	Msa1310300	0.800385	1.477817e-48	-8.615850e-47
Msa0633860	Msa1312730	0.811946	5.428121e-51	-8.615850e-47
Msa0633860	Msa1323060	0.817488	3.213656e-52	-8.615850e-47
Msa0633860	Msa1325650	0.809943	1.473590e-50	-8.615850e-47
Msa0633860	Msa1330240	0.863173	3.007004e-64	-8.615850e-47
Msa0633860	Msa1334650	0.818339	2.064210e-52	-8.615850e-47
Msa0633860	Msa1347030	0.931336	4.389954e-94	-8.615850e-47
Msa0633860	Msa1354040	0.817688	2.896418e-52	-8.615850e-47
Msa0633860	Msa1365210	0.817097	3.935226e-52	-8.615850e-47
Msa0633860	Msa1369860	0.856175	3.842155e-62	-8.615850e-47
Msa0633860	Msa1388610	0.818209	2.208289e-52	-8.615850e-47
Msa0633860	Msa1392680	0.806803	6.889538e-50	-8.615850e-47
Msa0633860	Msa1405770	0.817156	3.816181e-52	-8.615850e-47
Msa0633860	Msa1419930	0.808019	3.803893e-50	-8.615850e-47
Msa0633860	Msa1426870	0.815417	9.343445e-52	-8.615850e-47
Msa0633860	Msa1437150	0.803777	2.964550e-49	-8.615850e-47
Msa0122180	Msa0633860	0.824016	1.014042e-53	-8.615850e-47
Msa0122210	Msa0633860	0.801356	9.357086e-49	-8.615850e-47
Msa0123900	Msa0633860	0.806359	8.548987e-50	-8.615850e-47
Msa0141670	Msa0633860	0.802774	4.783076e-49	-8.615850e-47
Msa0150210	Msa0633860	0.826227	3.045581e-54	-8.615850e-47
Msa0157020	Msa0633860	0.826371	2.815090e-54	-8.615850e-47
Msa0202630	Msa0633860	0.801372	9.287851e-49	-8.615850e-47
Msa0207180	Msa0633860	0.801068	1.072081e-48	-8.615850e-47
Msa0224700	Msa0633860	0.804967	1.674969e-49	-8.615850e-47
Msa0227490	Msa0633860	0.809792	1.588558e-50	-8.615850e-47
Msa0227960	Msa0633860	0.812640	3.829388e-51	-8.615850e-47
Msa0230400	Msa0633860	0.841510	4.549510e-58	-8.615850e-47
Msa0380060	Msa0633860	0.817751	2.803760e-52	-8.615850e-47
Msa0383570	Msa0633860	0.816973	4.194406e-52	-8.615850e-47
Msa0413180	Msa0633860	0.815735	7.940182e-52	-8.615850e-47
Msa0429270	Msa0633860	0.829418	5.205580e-55	-8.615850e-47
Msa0274780	Msa0633860	0.847848	8.916570e-60	-8.615850e-47
Msa0315480	Msa0633860	0.817718	2.851532e-52	-8.615850e-47
Msa0320540	Msa0633860	0.801060	1.075904e-48	-8.615850e-47
Msa0485280	Msa0633860	0.814097	1.832559e-51	-8.615850e-47
Msa0519200	Msa0633860	0.808244	3.406113e-50	-8.615850e-47
Msa0537870	Msa0633860	0.933928	8.868213e-96	-8.615850e-47
Msa0537880	Msa0633860	0.996214	9.371571e-225	-8.615850e-47
Msa0538260	Msa0633860	0.909704	4.240853e-82	-8.615850e-47
Msa0538270	Msa0633860	0.905420	4.373322e-80	-8.615850e-47
Msa0538570	Msa0633860	0.855612	5.611715e-62	-8.615850e-47
Msa0560200	Msa0633860	0.813657	2.290492e-51	-8.615850e-47
Msa0561510	Msa0633860	0.805569	1.253256e-49	-8.615850e-47
Msa0581340	Msa0633860	0.801523	8.651240e-49	-8.615850e-47
Msa0594150	Msa0633860	0.828919	6.878111e-55	-8.615850e-47
Msa0594180	Msa0633860	0.834808	2.419278e-56	-8.615850e-47
Msa0594390	Msa0633860	0.850873	1.281367e-60	-8.615850e-47
Msa0595780	Msa0633860	0.808945	2.413317e-50	-8.615850e-47
Msa0600390	Msa0633860	0.832152	1.112410e-55	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0633860	MtrunA17_Chr4g0012951	85.047	107	16	0	1	107	1	107	1.39e-61	184
Msa0633860	MtrunA17_Chr4g0012991	85.047	107	16	0	1	107	56	162	5.14e-60	182
Msa0633860	MtrunA17_Chr4g0013001	84.112	107	17	0	1	107	1	107	1.09e-59	179
Msa0633860	MtrunA17_Chr4g0012961	83.178	107	18	0	1	107	1	107	3.52e-59	178
Msa0633860	MtrunA17_Chr4g0013261	62.281	114	40	2	1	112	1	113	6.15e-42	134
Msa0633860	MtrunA17_Chr4g0013251	62.281	114	40	2	1	112	1	113	5.60e-41	132
Msa0633860	MtrunA17_Chr4g0012901	48.598	107	53	2	1	105	5	111	5.19e-29	102
Msa0633860	MtrunA17_Chr4g0013211	44.545	110	61	0	1	110	1	110	1.72e-27	98.2
Msa0633860	MtrunA17_Chr7g0244691	45.045	111	58	2	4	112	7	116	5.63e-27	97.1
Msa0633860	MtrunA17_Chr7g0244831	44.348	115	59	4	2	112	3	116	2.92e-25	92.4
Msa0633860	MtrunA17_Chr4g0012891	42.609	115	59	3	5	112	9	123	9.15e-25	91.7
Msa0633860	MtrunA17_Chr7g0244641	40.367	109	63	2	4	110	7	115	1.40e-24	90.9
Msa0633860	MtrunA17_Chr7g0244671	38.739	111	66	1	4	112	7	117	1.41e-24	91.7
Msa0633860	MtrunA17_Chr7g0244841	42.342	111	60	3	4	112	7	115	2.12e-24	90.5
Msa0633860	MtrunA17_Chr2g0309241	42.991	107	59	2	1	106	1	106	1.07e-22	85.9
Msa0633860	MtrunA17_Chr4g0012931	39.823	113	65	1	1	110	1	113	5.14e-22	84.7
Msa0633860	MtrunA17_Chr7g0244811	38.835	103	61	1	1	101	5	107	9.72e-21	81.3
Msa0633860	MtrunA17_Chr7g0244741	40.179	112	64	1	4	112	7	118	6.33e-19	76.6
Msa0633860	MtrunA17_Chr4g0012921	39.823	113	65	2	1	110	1	113	1.59e-18	75.5
Msa0633860	MtrunA17_Chr7g0244681	40.367	109	62	2	4	110	7	114	3.05e-18	74.7
Msa0633860	MtrunA17_Chr3g0107481	35.780	109	69	1	5	112	3	111	3.34e-16	69.3
Msa0633860	MtrunA17_Chr7g0233431	30.841	107	73	1	5	110	20	126	8.41e-16	68.6
Msa0633860	MtrunA17_Chr7g0244721	36.364	110	66	3	4	110	7	115	1.58e-15	67.8
Msa0633860	MtrunA17_Chr7g0244821	37.615	109	64	4	4	110	7	113	1.75e-15	67.8
Msa0633860	MtrunA17_Chr7g0244761	37.615	109	65	3	4	110	32	139	2.14e-15	67.8
Msa0633860	MtrunA17_Chr7g0244791	36.036	111	64	2	4	110	12	119	8.27e-15	65.9
Msa0633860	MtrunA17_Chr1g0165761	40.741	108	61	2	5	110	8	114	1.00e-14	65.5
Msa0633860	MtrunA17_Chr3g0105001	36.111	108	61	4	4	104	7	113	2.85e-14	64.3
Msa0633860	MtrunA17_Chr3g0104971	36.111	108	61	4	4	104	7	113	5.99e-14	63.5
Msa0633860	MtrunA17_Chr3g0104981	36.111	108	61	4	4	104	7	113	6.25e-14	63.5
Msa0633860	MtrunA17_Chr3g0105021	37.864	103	56	4	9	104	12	113	6.67e-14	63.5
Msa0633860	MtrunA17_Chr3g0105011	37.864	103	56	4	9	104	12	113	6.67e-14	63.5
Msa0633860	MtrunA17_Chr7g0244661	38.889	108	58	3	4	109	7	108	1.62e-13	62.4
Msa0633860	MtrunA17_Chr3g0104991	37.864	103	56	4	9	104	12	113	1.73e-13	62.4
Msa0633860	MtrunA17_Chr3g0104951	35.185	108	62	4	4	104	7	113	1.79e-13	62.4
Msa0633860	MtrunA17_Chr3g0104931	35.185	108	62	4	4	104	7	113	1.79e-13	62.4
Msa0633860	MtrunA17_Chr3g0104941	35.185	108	62	4	4	104	7	113	1.79e-13	62.4
Msa0633860	MtrunA17_Chr3g0104921	36.893	103	57	4	9	104	2	103	2.59e-13	61.6
Msa0633860	MtrunA17_Chr3g0104911	36.893	103	57	4	9	104	2	103	2.59e-13	61.6
Msa0633860	MtrunA17_Chr3g0105031	37.143	105	58	4	9	106	2	105	2.69e-13	61.6
Msa0633860	MtrunA17_Chr3g0104901	35.088	114	63	5	1	104	1	113	3.15e-13	61.6
Msa0633860	MtrunA17_Chr3g0095991	34.906	106	63	4	3	104	10	113	5.48e-13	61.2
Msa0633860	MtrunA17_Chr3g0105041	35.185	108	62	4	4	104	7	113	6.11e-13	61.2
Msa0633860	MtrunA17_Chr7g0244751	33.636	110	72	1	4	112	7	116	6.64e-13	60.8
Msa0633860	MtrunA17_Chr3g0104961	35.185	108	62	4	4	104	7	113	1.19e-12	60.5
Msa0633860	MtrunA17_Chr3g0095931	31.683	101	63	3	10	105	13	112	1.56e-12	59.7
Msa0633860	MtrunA17_Chr3g0101431	35.922	103	58	4	9	104	11	112	2.35e-12	59.7
Msa0633860	MtrunA17_Chr3g0095951	32.407	108	65	4	4	104	7	113	5.12e-12	58.5
Msa0633860	MtrunA17_Chr3g0096021	32.407	108	65	4	4	104	7	113	5.88e-12	58.5
Msa0633860	MtrunA17_Chr3g0096031	32.407	108	65	4	4	104	7	113	1.01e-11	57.8
Msa0633860	MtrunA17_Chr3g0094521	30.189	106	68	4	10	112	18	120	2.42e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0633860	AT5G59320.1	59.596	99	38	1	8	106	10	106	4.36e-35	117
Msa0633860	AT2G38540.1	61.111	108	36	6	4	107	5	110	5.39e-32	110
Msa0633860	AT5G59310.1	56.000	100	39	2	8	107	10	104	7.64e-31	107
Msa0633860	AT2G38530.1	58.036	112	43	4	4	112	5	115	1.76e-29	103
Msa0633860	AT3G51600.1	52.336	107	47	4	4	107	5	110	1.50e-25	93.6
Msa0633860	AT2G15050.3	47.115	104	51	3	4	103	5	108	1.93e-25	93.6
Msa0633860	AT2G15050.2	47.115	104	51	3	4	103	5	108	1.97e-25	93.2
Msa0633860	AT2G15050.1	47.115	104	51	3	4	103	5	108	2.51e-25	93.2
Msa0633860	AT3G51590.1	45.098	102	53	3	6	106	8	107	1.82e-24	90.9
Msa0633860	AT4G33355.1	53.846	78	36	0	24	101	28	105	6.05e-22	84.3
Msa0633860	AT4G33355.2	53.846	78	36	0	24	101	28	105	6.26e-22	84.3
Msa0633860	AT5G01870.1	44.231	104	50	4	9	105	4	106	2.55e-21	82.8
Msa0633860	AT3G08770.1	45.455	88	44	3	22	106	18	104	9.34e-18	73.6
Msa0633860	AT3G08770.2	45.455	88	44	3	22	106	18	104	1.11e-17	73.6
Msa0633860	AT2G18370.1	40.449	89	51	2	15	102	16	103	3.05e-16	69.7

Find 46 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTTACTTAGTGAAATATTT+TGG	0.134106	4_3:+20041806	Msa0633860:three_prime_UTR
AGTGCTGCTGGTGCTATTTC+TGG	0.136175	4_3:+20041052	Msa0633860:CDS
TTATTCTTAATAGCATTAAA+TGG	0.274471	4_3:-20041689	None:intergenic
CCATGCATTGCATATCTTAG+AGG	0.320070	4_3:+20040929	Msa0633860:CDS
GTTGCAGCTCCAAAGTTATA+TGG	0.346531	4_3:+20041640	Msa0633860:CDS
AATGTTAACACCGCATTTGC+CGG	0.346833	4_3:-20041104	None:intergenic
CTTCTTATACCAGACTGTAT+TGG	0.369553	4_3:+20041753	Msa0633860:three_prime_UTR
CCATCTCCAGCATGCTGTGC+TGG	0.375274	4_3:+20040962	Msa0633860:CDS
AACACAGCTGCTGGTCTTCC+CGG	0.392611	4_3:+20041085	Msa0633860:CDS
TTGCAAGCAGCCTGACGGTC+AGG	0.421711	4_3:-20041019	None:intergenic
ATCTCATGTGGAACAGTAAC+TGG	0.426922	4_3:+20040896	Msa0633860:CDS
GAGAAGTTTCCATATAACTT+TGG	0.427067	4_3:-20041649	None:intergenic
GGTAGAGGTACTGATCTTGT+AGG	0.430936	4_3:-20041128	None:intergenic
AAGTCAAAGGAAATAAAGTT+GGG	0.431539	4_3:+20041953	Msa0633860:three_prime_UTR
TAAGTCAAAGGAAATAAAGT+TGG	0.441141	4_3:+20041952	Msa0633860:three_prime_UTR
CACCACCACGCCTGACCGTC+AGG	0.443141	4_3:+20041009	Msa0633860:CDS
ACGGTCAGGCGTGGTGGTGG+CGG	0.445777	4_3:-20041005	None:intergenic
GTCAGGCGTGGTGGTGGCGG+CGG	0.446691	4_3:-20041002	None:intergenic
CAGTCTGGTATAAGAAGGAT+AGG	0.473305	4_3:-20041747	None:intergenic
TTCACTCCAGCACAGCATGC+TGG	0.485039	4_3:-20040968	None:intergenic
AACTGCTTGAAAAGTGCTGC+TGG	0.486030	4_3:+20041040	Msa0633860:CDS
ATGTTAACACCGCATTTGCC+GGG	0.493990	4_3:-20041103	None:intergenic
GCATATCTTAGAGGTGGTGC+TGG	0.504129	4_3:+20040938	Msa0633860:CDS
CTGACGGTCAGGCGTGGTGG+TGG	0.517462	4_3:-20041008	None:intergenic
AAGAATAATAATGAGACTGA+TGG	0.524929	4_3:+20041704	Msa0633860:three_prime_UTR
GTGCATGATCGTTGCACCTA+TGG	0.527274	4_3:+20040862	Msa0633860:CDS
CAATACAGTCTGGTATAAGA+AGG	0.533082	4_3:-20041752	None:intergenic
CCAGCACAGCATGCTGGAGA+TGG	0.550113	4_3:-20040962	None:intergenic
TTGAATGCTAACACAGCTGC+TGG	0.551046	4_3:+20041076	Msa0633860:CDS
TCTATCTACTAATAAGTCAA+AGG	0.554766	4_3:+20041940	Msa0633860:three_prime_UTR
GTAGAGGTACTGATCTTGTA+GGG	0.565695	4_3:-20041127	None:intergenic
AGCAGCCTGACGGTCAGGCG+TGG	0.565783	4_3:-20041014	None:intergenic
TAAGACAATCCAATACAGTC+TGG	0.567330	4_3:-20041762	None:intergenic
TGGTTATATTTACAGCATCA+GGG	0.568642	4_3:+20041605	Msa0633860:intron
AGCCTGACGGTCAGGCGTGG+TGG	0.576862	4_3:-20041011	None:intergenic
GAGATTGCAGCATCTGCCAT+AGG	0.577723	4_3:-20040878	None:intergenic
CCTCTAAGATATGCAATGCA+TGG	0.588805	4_3:-20040929	None:intergenic
GCTGGTCTTCCCGGCAAATG+CGG	0.592170	4_3:+20041094	Msa0633860:CDS
TGCATTGCATATCTTAGAGG+TGG	0.623803	4_3:+20040932	Msa0633860:CDS
AGCAGTTGCAAGCAGCCTGA+CGG	0.624471	4_3:-20041024	None:intergenic
AATAATAATGAGACTGATGG+AGG	0.629956	4_3:+20041707	Msa0633860:three_prime_UTR
ATGGAGGCTCATCTTATGCG+TGG	0.640602	4_3:+20041723	Msa0633860:three_prime_UTR
AGTCTGGTATAAGAAGGATA+GGG	0.652754	4_3:-20041746	None:intergenic
GCAGATGCTGCAATCTCATG+TGG	0.654132	4_3:+20040884	Msa0633860:CDS
AGTCAAAGGAAATAAAGTTG+GGG	0.655094	4_3:+20041954	Msa0633860:three_prime_UTR
ATAAGAGTAATGACATAACC+AGG	0.665259	4_3:-20041836	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTATTGGTTATTTATTTTT+TGG	+	chr4_3:20041512-20041531	Msa0633860:intron	10.0%
!!!	TTTATTGGTTATTTATTTTT+TGG	+	chr4_3:20041512-20041531	Msa0633860:intron	10.0%
!!!	TTTTTCTTACTTCTAAAATT+TGG	-	chr4_3:20041368-20041387	None:intergenic	15.0%
!!!	AAACATTTTTGTGAATTTTT+TGG	+	chr4_3:20041430-20041449	Msa0633860:intron	15.0%
!!!	TTATTTTTTGGTGTTATTAT+TGG	+	chr4_3:20041524-20041543	Msa0633860:intron	15.0%
!!	TTATTCTTAATAGCATTAAA+TGG	-	chr4_3:20041692-20041711	None:intergenic	15.0%
!!!	TTTTTCTTACTTCTAAAATT+TGG	-	chr4_3:20041368-20041387	None:intergenic	15.0%
!!!	AAACATTTTTGTGAATTTTT+TGG	+	chr4_3:20041430-20041449	Msa0633860:intron	15.0%
!!!	TTATTTTTTGGTGTTATTAT+TGG	+	chr4_3:20041524-20041543	Msa0633860:intron	15.0%
!!	TTATTCTTAATAGCATTAAA+TGG	-	chr4_3:20041692-20041711	None:intergenic	15.0%
!!!	TTATTGGTTAATATTCAAGT+CGG	+	chr4_3:20041540-20041559	Msa0633860:intron	20.0%
!!!	TGACTAGTTTTTTTTTTGTT+TGG	+	chr4_3:20041585-20041604	Msa0633860:intron	20.0%
!!!	AGTTACTTAGTGAAATATTT+TGG	+	chr4_3:20041806-20041825	Msa0633860:three_prime_UTR	20.0%
!!!	GTTACTTAGTGAAATATTTT+GGG	+	chr4_3:20041807-20041826	Msa0633860:three_prime_UTR	20.0%
!!!	TTATTGGTTAATATTCAAGT+CGG	+	chr4_3:20041540-20041559	Msa0633860:intron	20.0%
!!!	TGACTAGTTTTTTTTTTGTT+TGG	+	chr4_3:20041585-20041604	Msa0633860:intron	20.0%
!!!	AGTTACTTAGTGAAATATTT+TGG	+	chr4_3:20041806-20041825	Msa0633860:three_prime_UTR	20.0%
!!!	GTTACTTAGTGAAATATTTT+GGG	+	chr4_3:20041807-20041826	Msa0633860:three_prime_UTR	20.0%
!	GAAAAATATAGTAAGAGTGA+AGG	-	chr4_3:20041185-20041204	None:intergenic	25.0%
!	AAGAATAATAATGAGACTGA+TGG	+	chr4_3:20041704-20041723	Msa0633860:three_prime_UTR	25.0%
!	TCTATCTACTAATAAGTCAA+AGG	+	chr4_3:20041940-20041959	Msa0633860:three_prime_UTR	25.0%
!	TAAGTCAAAGGAAATAAAGT+TGG	+	chr4_3:20041952-20041971	Msa0633860:three_prime_UTR	25.0%
!	AAGTCAAAGGAAATAAAGTT+GGG	+	chr4_3:20041953-20041972	Msa0633860:three_prime_UTR	25.0%
!	GAAAAATATAGTAAGAGTGA+AGG	-	chr4_3:20041185-20041204	None:intergenic	25.0%
!	AAGAATAATAATGAGACTGA+TGG	+	chr4_3:20041704-20041723	Msa0633860:three_prime_UTR	25.0%
!	TCTATCTACTAATAAGTCAA+AGG	+	chr4_3:20041940-20041959	Msa0633860:three_prime_UTR	25.0%
!	TAAGTCAAAGGAAATAAAGT+TGG	+	chr4_3:20041952-20041971	Msa0633860:three_prime_UTR	25.0%
!	AAGTCAAAGGAAATAAAGTT+GGG	+	chr4_3:20041953-20041972	Msa0633860:three_prime_UTR	25.0%
	TTAAGAAACGCTGTATAAAG+AGG	+	chr4_3:20041216-20041235	Msa0633860:intron	30.0%
	TTGGTTATATTTACAGCATC+AGG	+	chr4_3:20041604-20041623	Msa0633860:intron	30.0%
	TGGTTATATTTACAGCATCA+GGG	+	chr4_3:20041605-20041624	Msa0633860:intron	30.0%
	GAGAAGTTTCCATATAACTT+TGG	-	chr4_3:20041652-20041671	None:intergenic	30.0%
	AATAATAATGAGACTGATGG+AGG	+	chr4_3:20041707-20041726	Msa0633860:three_prime_UTR	30.0%
!!!	AAATATTTTGGGTTTGTACC+TGG	+	chr4_3:20041818-20041837	Msa0633860:three_prime_UTR	30.0%
	ATAAGAGTAATGACATAACC+AGG	-	chr4_3:20041839-20041858	None:intergenic	30.0%
	AGTCAAAGGAAATAAAGTTG+GGG	+	chr4_3:20041954-20041973	Msa0633860:three_prime_UTR	30.0%
	TTAAGAAACGCTGTATAAAG+AGG	+	chr4_3:20041216-20041235	Msa0633860:intron	30.0%
	TTGGTTATATTTACAGCATC+AGG	+	chr4_3:20041604-20041623	Msa0633860:intron	30.0%
	TGGTTATATTTACAGCATCA+GGG	+	chr4_3:20041605-20041624	Msa0633860:intron	30.0%
	GAGAAGTTTCCATATAACTT+TGG	-	chr4_3:20041652-20041671	None:intergenic	30.0%
	AATAATAATGAGACTGATGG+AGG	+	chr4_3:20041707-20041726	Msa0633860:three_prime_UTR	30.0%
!!!	AAATATTTTGGGTTTGTACC+TGG	+	chr4_3:20041818-20041837	Msa0633860:three_prime_UTR	30.0%
	ATAAGAGTAATGACATAACC+AGG	-	chr4_3:20041839-20041858	None:intergenic	30.0%
	AGTCAAAGGAAATAAAGTTG+GGG	+	chr4_3:20041954-20041973	Msa0633860:three_prime_UTR	30.0%
	CTGATCTACTGCTTCTTTAT+TGG	+	chr4_3:20041497-20041516	Msa0633860:intron	35.0%
	AGTCTGGTATAAGAAGGATA+GGG	-	chr4_3:20041749-20041768	None:intergenic	35.0%
	CAATACAGTCTGGTATAAGA+AGG	-	chr4_3:20041755-20041774	None:intergenic	35.0%
	CTTCTTATACCAGACTGTAT+TGG	+	chr4_3:20041753-20041772	Msa0633860:three_prime_UTR	35.0%
	TAAGACAATCCAATACAGTC+TGG	-	chr4_3:20041765-20041784	None:intergenic	35.0%
	CTGATCTACTGCTTCTTTAT+TGG	+	chr4_3:20041497-20041516	Msa0633860:intron	35.0%
	AGTCTGGTATAAGAAGGATA+GGG	-	chr4_3:20041749-20041768	None:intergenic	35.0%
	CAATACAGTCTGGTATAAGA+AGG	-	chr4_3:20041755-20041774	None:intergenic	35.0%
	CTTCTTATACCAGACTGTAT+TGG	+	chr4_3:20041753-20041772	Msa0633860:three_prime_UTR	35.0%
	TAAGACAATCCAATACAGTC+TGG	-	chr4_3:20041765-20041784	None:intergenic	35.0%
	ATCTCATGTGGAACAGTAAC+TGG	+	chr4_3:20040896-20040915	Msa0633860:CDS	40.0%
!!	CCTCTAAGATATGCAATGCA+TGG	-	chr4_3:20040932-20040951	None:intergenic	40.0%
	CCATGCATTGCATATCTTAG+AGG	+	chr4_3:20040929-20040948	Msa0633860:CDS	40.0%
	TGCATTGCATATCTTAGAGG+TGG	+	chr4_3:20040932-20040951	Msa0633860:CDS	40.0%
	AATGTTAACACCGCATTTGC+CGG	-	chr4_3:20041107-20041126	None:intergenic	40.0%
!!	GTAGAGGTACTGATCTTGTA+GGG	-	chr4_3:20041130-20041149	None:intergenic	40.0%
	AAGAACTTACGTAGCACAGT+TGG	-	chr4_3:20041152-20041171	None:intergenic	40.0%
	AACGCTGTATAAAGAGGTCT+AGG	+	chr4_3:20041222-20041241	Msa0633860:intron	40.0%
	TATTCAAGTCGGTCGAAAAG+TGG	+	chr4_3:20041551-20041570	Msa0633860:intron	40.0%
	GTTGCAGCTCCAAAGTTATA+TGG	+	chr4_3:20041640-20041659	Msa0633860:CDS	40.0%
	CAGTCTGGTATAAGAAGGAT+AGG	-	chr4_3:20041750-20041769	None:intergenic	40.0%
	ATCTCATGTGGAACAGTAAC+TGG	+	chr4_3:20040896-20040915	Msa0633860:CDS	40.0%
!!	CCTCTAAGATATGCAATGCA+TGG	-	chr4_3:20040932-20040951	None:intergenic	40.0%
	CCATGCATTGCATATCTTAG+AGG	+	chr4_3:20040929-20040948	Msa0633860:CDS	40.0%
	TGCATTGCATATCTTAGAGG+TGG	+	chr4_3:20040932-20040951	Msa0633860:CDS	40.0%
	AATGTTAACACCGCATTTGC+CGG	-	chr4_3:20041107-20041126	None:intergenic	40.0%
!!	GTAGAGGTACTGATCTTGTA+GGG	-	chr4_3:20041130-20041149	None:intergenic	40.0%
	AAGAACTTACGTAGCACAGT+TGG	-	chr4_3:20041152-20041171	None:intergenic	40.0%
	AACGCTGTATAAAGAGGTCT+AGG	+	chr4_3:20041222-20041241	Msa0633860:intron	40.0%
	TATTCAAGTCGGTCGAAAAG+TGG	+	chr4_3:20041551-20041570	Msa0633860:intron	40.0%
	GTTGCAGCTCCAAAGTTATA+TGG	+	chr4_3:20041640-20041659	Msa0633860:CDS	40.0%
	CAGTCTGGTATAAGAAGGAT+AGG	-	chr4_3:20041750-20041769	None:intergenic	40.0%
!!	AACTGCTTGAAAAGTGCTGC+TGG	+	chr4_3:20041040-20041059	Msa0633860:CDS	45.0%
	TTGAATGCTAACACAGCTGC+TGG	+	chr4_3:20041076-20041095	Msa0633860:CDS	45.0%
	ATGTTAACACCGCATTTGCC+GGG	-	chr4_3:20041106-20041125	None:intergenic	45.0%
!!	GGTAGAGGTACTGATCTTGT+AGG	-	chr4_3:20041131-20041150	None:intergenic	45.0%
!!	TTACGTAGCACAGTTGGTAG+AGG	-	chr4_3:20041146-20041165	None:intergenic	45.0%
!!	AACTGCTTGAAAAGTGCTGC+TGG	+	chr4_3:20041040-20041059	Msa0633860:CDS	45.0%
	TTGAATGCTAACACAGCTGC+TGG	+	chr4_3:20041076-20041095	Msa0633860:CDS	45.0%
	ATGTTAACACCGCATTTGCC+GGG	-	chr4_3:20041106-20041125	None:intergenic	45.0%
!!	GGTAGAGGTACTGATCTTGT+AGG	-	chr4_3:20041131-20041150	None:intergenic	45.0%
!!	TTACGTAGCACAGTTGGTAG+AGG	-	chr4_3:20041146-20041165	None:intergenic	45.0%
!!!	AAAAATTATAATATTTTTAG+AGG	+	chr4_3:20041383-20041402	Msa0633860:intron	5.0%
!!!	CTTTATAATAATTTTTTTTT+AGG	+	chr4_3:20041459-20041478	Msa0633860:intron	5.0%
!!!	AAAAATTATAATATTTTTAG+AGG	+	chr4_3:20041383-20041402	Msa0633860:intron	5.0%
!!!	CTTTATAATAATTTTTTTTT+AGG	+	chr4_3:20041459-20041478	Msa0633860:intron	5.0%
	GTGCATGATCGTTGCACCTA+TGG	+	chr4_3:20040862-20040881	Msa0633860:CDS	50.0%
	GAGATTGCAGCATCTGCCAT+AGG	-	chr4_3:20040881-20040900	None:intergenic	50.0%
	GCAGATGCTGCAATCTCATG+TGG	+	chr4_3:20040884-20040903	Msa0633860:CDS	50.0%
	GCATATCTTAGAGGTGGTGC+TGG	+	chr4_3:20040938-20040957	Msa0633860:CDS	50.0%
!!	AGTGCTGCTGGTGCTATTTC+TGG	+	chr4_3:20041052-20041071	Msa0633860:CDS	50.0%
	ATGGAGGCTCATCTTATGCG+TGG	+	chr4_3:20041723-20041742	Msa0633860:three_prime_UTR	50.0%
	GTGCATGATCGTTGCACCTA+TGG	+	chr4_3:20040862-20040881	Msa0633860:CDS	50.0%
	GAGATTGCAGCATCTGCCAT+AGG	-	chr4_3:20040881-20040900	None:intergenic	50.0%
	GCAGATGCTGCAATCTCATG+TGG	+	chr4_3:20040884-20040903	Msa0633860:CDS	50.0%
	GCATATCTTAGAGGTGGTGC+TGG	+	chr4_3:20040938-20040957	Msa0633860:CDS	50.0%
!!	AGTGCTGCTGGTGCTATTTC+TGG	+	chr4_3:20041052-20041071	Msa0633860:CDS	50.0%
	ATGGAGGCTCATCTTATGCG+TGG	+	chr4_3:20041723-20041742	Msa0633860:three_prime_UTR	50.0%
	TTCACTCCAGCACAGCATGC+TGG	-	chr4_3:20040971-20040990	None:intergenic	55.0%
	AGCAGTTGCAAGCAGCCTGA+CGG	-	chr4_3:20041027-20041046	None:intergenic	55.0%
!!	AACACAGCTGCTGGTCTTCC+CGG	+	chr4_3:20041085-20041104	Msa0633860:CDS	55.0%
	TTCACTCCAGCACAGCATGC+TGG	-	chr4_3:20040971-20040990	None:intergenic	55.0%
	AGCAGTTGCAAGCAGCCTGA+CGG	-	chr4_3:20041027-20041046	None:intergenic	55.0%
!!	AACACAGCTGCTGGTCTTCC+CGG	+	chr4_3:20041085-20041104	Msa0633860:CDS	55.0%
	CCAGCACAGCATGCTGGAGA+TGG	-	chr4_3:20040965-20040984	None:intergenic	60.0%
	CCATCTCCAGCATGCTGTGC+TGG	+	chr4_3:20040962-20040981	Msa0633860:CDS	60.0%
	TTGCAAGCAGCCTGACGGTC+AGG	-	chr4_3:20041022-20041041	None:intergenic	60.0%
!!	GCTGGTCTTCCCGGCAAATG+CGG	+	chr4_3:20041094-20041113	Msa0633860:CDS	60.0%
	CCAGCACAGCATGCTGGAGA+TGG	-	chr4_3:20040965-20040984	None:intergenic	60.0%
	CCATCTCCAGCATGCTGTGC+TGG	+	chr4_3:20040962-20040981	Msa0633860:CDS	60.0%
	TTGCAAGCAGCCTGACGGTC+AGG	-	chr4_3:20041022-20041041	None:intergenic	60.0%
!!	GCTGGTCTTCCCGGCAAATG+CGG	+	chr4_3:20041094-20041113	Msa0633860:CDS	60.0%
!!	ACGGTCAGGCGTGGTGGTGG+CGG	-	chr4_3:20041008-20041027	None:intergenic	70.0%
	CTGACGGTCAGGCGTGGTGG+TGG	-	chr4_3:20041011-20041030	None:intergenic	70.0%
	CACCACCACGCCTGACCGTC+AGG	+	chr4_3:20041009-20041028	Msa0633860:CDS	70.0%
	AGCCTGACGGTCAGGCGTGG+TGG	-	chr4_3:20041014-20041033	None:intergenic	70.0%
	AGCAGCCTGACGGTCAGGCG+TGG	-	chr4_3:20041017-20041036	None:intergenic	70.0%
!!	ACGGTCAGGCGTGGTGGTGG+CGG	-	chr4_3:20041008-20041027	None:intergenic	70.0%
	CTGACGGTCAGGCGTGGTGG+TGG	-	chr4_3:20041011-20041030	None:intergenic	70.0%
	CACCACCACGCCTGACCGTC+AGG	+	chr4_3:20041009-20041028	Msa0633860:CDS	70.0%
	AGCCTGACGGTCAGGCGTGG+TGG	-	chr4_3:20041014-20041033	None:intergenic	70.0%
	AGCAGCCTGACGGTCAGGCG+TGG	-	chr4_3:20041017-20041036	None:intergenic	70.0%
!!	GTCAGGCGTGGTGGTGGCGG+CGG	-	chr4_3:20041005-20041024	None:intergenic	75.0%
!!	GTCAGGCGTGGTGGTGGCGG+CGG	-	chr4_3:20041005-20041024	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	20040824	20042005	20040824	ID=Msa0633860;Name=Msa0633860
chr4_3	mRNA	20040824	20042005	20040824	ID=Msa0633860-mRNA-1;Parent=Msa0633860;Name=Msa0633860-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|0\|0.5\|1\|1\|1\|2\|309\|130
chr4_3	exon	20040824	20041139	20040824	ID=Msa0633860-mRNA-1:exon:5395;Parent=Msa0633860-mRNA-1
chr4_3	exon	20041620	20042005	20041620	ID=Msa0633860-mRNA-1:exon:5396;Parent=Msa0633860-mRNA-1
chr4_3	CDS	20040824	20041139	20040824	ID=Msa0633860-mRNA-1:cds;Parent=Msa0633860-mRNA-1
chr4_3	CDS	20041620	20041696	20041620	ID=Msa0633860-mRNA-1:cds;Parent=Msa0633860-mRNA-1
chr4_3	three_prime_UTR	20041697	20042005	20041697	ID=Msa0633860-mRNA-1:three_prime_utr;Parent=Msa0633860-mRNA-1

Gene Sequence

>Msa0633860

ATGGCAAGCATGAAGGTAGCATGTGTTGTTTTGATGATGTGCATGATCGTTGCACCTATGGCAGATGCTGCAATCTCATGTGGAACAGTAACTGGTGCTCTTTCTCCATGCATTGCATATCTTAGAGGTGGTGCTGGTCCATCTCCAGCATGCTGTGCTGGAGTGAAGAGACTTAACGCCGCCGCCACCACCACGCCTGACCGTCAGGCTGCTTGCAACTGCTTGAAAAGTGCTGCTGGTGCTATTTCTGGTTTGAATGCTAACACAGCTGCTGGTCTTCCCGGCAAATGCGGTGTTAACATTCCCTACAAGATCACATCAGGGCTTGAAGATAATGTTGCAGCTCCAAAGTTATATGGAAACTTCTCACTAGTATATGAGAGTGCCATTTAA

Protein sequence

>Msa0633860

MASMKVACVVLMMCMIVAPMADAAISCGTVTGALSPCIAYLRGGAGPSPACCAGVKRLNAAATTTPDRQAACNCLKSAAGAISGLNANTAAGLPGKCGVNIPYKITSGLEDNVAAPKLYGNFSLVYESAI*