AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0979850


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0979850 MtrunA17_Chr1g0207391 96.183 393 15 0 27 419 31 423 0.0 779
Msa0979850 MtrunA17_Chr7g0226311 40.841 333 183 4 86 415 11 332 6.50e-84 260
Msa0979850 MtrunA17_Chr6g0477141 42.763 304 172 1 64 365 120 423 4.51e-81 257
Msa0979850 MtrunA17_Chr6g0478531 39.945 363 206 3 65 416 19 380 8.65e-81 254
Msa0979850 MtrunA17_Chr8g0391981 40.566 318 176 5 90 399 83 395 2.39e-76 243
Msa0979850 MtrunA17_Chr1g0172731 34.557 327 200 7 49 365 28 350 5.44e-62 206
Msa0979850 MtrunA17_Chr1g0190171 34.557 327 200 7 49 365 28 350 6.87e-62 205
Msa0979850 MtrunA17_Chr1g0182341 32.039 309 172 6 69 365 45 327 1.20e-46 164
Msa0979850 MtrunA17_Chr2g0321401 30.508 236 148 6 49 271 27 259 1.43e-32 124
Msa0979850 MtrunA17_Chr2g0285471 32.474 194 118 5 88 271 1 191 1.00e-29 114
Msa0979850 MtrunA17_Chr8g0334561 28.692 237 153 6 48 271 27 260 7.56e-29 114
Msa0979850 MtrunA17_Chr4g0018411 39.216 153 80 4 266 416 9 150 2.48e-28 109
Msa0979850 MtrunA17_Chr7g0267201 33.750 160 96 2 91 240 1 160 1.31e-22 94.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0979850 AT5G35695.1 61.458 192 58 4 234 420 26 206 6.79e-76 235
Msa0979850 AT5G41980.1 36.193 373 208 8 55 416 11 364 8.32e-65 212
Msa0979850 AT1G43722.1 34.074 270 167 4 70 329 51 319 2.41e-44 157
Msa0979850 AT5G28950.1 70.408 98 28 1 167 263 1 98 1.52e-41 144
Msa0979850 AT3G30525.1 34.591 159 97 3 195 347 1 158 3.99e-24 98.6
Msa0979850 AT5G28730.1 27.311 238 144 4 86 318 25 238 6.02e-21 92.8
Msa0979850 AT3G55350.1 22.801 307 189 10 81 358 68 355 8.94e-13 70.1

Find 51 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCGTCATGCGTCTTTA+AGG 0.095606 6_4:-17184478 Msa0979850:CDS
GTAATTCGTCGTCGAGTTAA+TGG 0.220577 6_4:+17185410 None:intergenic
TTATACAATATCGAGCATTT+TGG 0.240408 6_4:+17185201 None:intergenic
TATATACATAAATCATTAAA+TGG 0.273521 6_4:-17185371 Msa0979850:CDS
AATGTGGTTGAGAGAATATT+TGG 0.317602 6_4:-17184455 Msa0979850:CDS
TCATTATTGTCTTCATGAAT+AGG 0.320777 6_4:+17184257 None:intergenic
AGCCATATACATGTATATTA+TGG 0.329326 6_4:-17185501 Msa0979850:CDS
TGCCATAATATACATGTATA+TGG 0.329982 6_4:+17185499 None:intergenic
GATACAAGATTTATTTGTAT+TGG 0.337150 6_4:-17185257 Msa0979850:CDS
CTCAATAGCACCTGATTTGA+TGG 0.344546 6_4:-17185108 Msa0979850:CDS
AGCTGCTTGTAATTTCGATT+TGG 0.365004 6_4:-17184816 Msa0979850:CDS
CAAAGCATCATTTAGCAACT+TGG 0.377464 6_4:+17184748 None:intergenic
GATTGCATTGGAGCTATTGA+TGG 0.391567 6_4:-17184920 Msa0979850:CDS
AACGAGAAGATGCTAATCTA+TGG 0.395264 6_4:-17184205 Msa0979850:CDS
CATTTAGCAACTTGGAATCA+TGG 0.400539 6_4:+17184756 None:intergenic
TGTATAATTAGAAATACATT+TGG 0.402713 6_4:-17185185 Msa0979850:CDS
AATCTATGGAGGACTAATAT+AGG 0.403864 6_4:-17184191 Msa0979850:CDS
GTTGGTTTAGCCATCAAATC+AGG 0.412864 6_4:+17185098 None:intergenic
GCGTCGTTCTTACAGATTGT+CGG 0.432319 6_4:-17185224 Msa0979850:CDS
GACTCGTTAGAATGTTCAAC+TGG 0.440308 6_4:+17184302 None:intergenic
CATATTCCTGCATTGGTAAG+AGG 0.460409 6_4:-17184893 Msa0979850:CDS
TGCTAAATGATGCTTTGAAA+AGG 0.482649 6_4:-17184742 Msa0979850:CDS
GATGCTTTGAAAAGGAAGAA+TGG 0.484883 6_4:-17184734 Msa0979850:CDS
TCGACGACGAATTACAAGTA+AGG 0.491797 6_4:-17185402 Msa0979850:CDS
CACTTGCAGCAAATTGTGAT+CGG 0.496654 6_4:+17185162 None:intergenic
CATAACTTTCGAAAGACATC+AGG 0.498455 6_4:+17185311 None:intergenic
ATTTAGCAACTTGGAATCAT+GGG 0.505901 6_4:+17184757 None:intergenic
CTAATATAGGTTCAGATATG+TGG 0.511004 6_4:-17184178 Msa0979850:CDS
TGATCACATACCTTGAAGTA+AGG 0.519561 6_4:+17185032 None:intergenic
TTTGCAGGCACACTTGAGGT+TGG 0.522108 6_4:+17185080 None:intergenic
CCATGTACGTTCTTAGCGGG+TGG 0.526839 6_4:-17184790 Msa0979850:CDS
TATGTGGAGAGATGCTACTT+AGG 0.527173 6_4:-17184162 None:intergenic
TCTCGTCCTCTTACCAATGC+AGG 0.530406 6_4:+17184887 None:intergenic
TAATGACCCTGAAAATGAAA+AGG 0.542906 6_4:-17184510 Msa0979850:CDS
AGTCCATGTACGTTCTTAGC+GGG 0.544261 6_4:-17184793 Msa0979850:CDS
GTACGTTCTTAGCGGGTGGG+AGG 0.544285 6_4:-17184786 Msa0979850:CDS
TACGTTCTTAGCGGGTGGGA+GGG 0.546796 6_4:-17184785 Msa0979850:CDS
GAGTCCATGTACGTTCTTAG+CGG 0.547275 6_4:-17184794 Msa0979850:CDS
GACACAAGCAGAACTTGTGT+TGG 0.559273 6_4:-17184378 Msa0979850:CDS
TGGTACACATATTCCTGCAT+TGG 0.559514 6_4:-17184900 Msa0979850:CDS
CATGTACGTTCTTAGCGGGT+GGG 0.565324 6_4:-17184789 Msa0979850:CDS
TCATGCGTCTTTAAGGAATG+TGG 0.573917 6_4:-17184471 Msa0979850:CDS
ATGTTCAACTGGAAATTCAT+CGG 0.590852 6_4:+17184313 None:intergenic
CGACGACGAATTACAAGTAA+GGG 0.598127 6_4:-17185401 Msa0979850:CDS
AGCAGTTATCGTGATCGTCA+TGG 0.601808 6_4:-17184860 Msa0979850:CDS
ATAAATCTTGTATCCACCAA+AGG 0.606437 6_4:+17185266 None:intergenic
TGATATCGCACACCTCGAAG+TGG 0.613190 6_4:+17184557 None:intergenic
GTTATCGTGATCGTCATGGA+AGG 0.625280 6_4:-17184856 Msa0979850:CDS
AATTCATGCTTTAATGCACG+TGG 0.633739 6_4:-17185477 Msa0979850:CDS
GAGAAGATGCTAATCTATGG+AGG 0.639211 6_4:-17184202 Msa0979850:CDS
AATGGACTAAAAGTGCCCCA+CGG 0.730602 6_4:-17184716 Msa0979850:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAATAAATTTTTTTTA+TGG - chr6_4:17184785-17184804 Msa0979850:CDS 0.0%
!! TATATACATAAATCATTAAA+TGG - chr6_4:17184379-17184398 Msa0979850:CDS 10.0%
!!! TTATATATAAACAAACTTTT+AGG - chr6_4:17184808-17184827 Msa0979850:CDS 10.0%
!! TGTATAATTAGAAATACATT+TGG - chr6_4:17184565-17184584 Msa0979850:CDS 15.0%
!!! TTAATTAGGTAAATATTTTC+TGG - chr6_4:17185132-17185151 Msa0979850:CDS 15.0%
!!! TAATTAGGTAAATATTTTCT+GGG - chr6_4:17185133-17185152 Msa0979850:CDS 15.0%
!! GATACAAGATTTATTTGTAT+TGG - chr6_4:17184493-17184512 Msa0979850:CDS 20.0%
!!! AAATTTTCACAAGATTTTGA+AGG - chr6_4:17184612-17184631 Msa0979850:CDS 20.0%
!! AAAAATTAATTACTTACCGT+GGG + chr6_4:17185053-17185072 None:intergenic 20.0%
!! TAAAAATTAATTACTTACCG+TGG + chr6_4:17185054-17185073 None:intergenic 20.0%
!! TCTGTATTGTCAATTTAATT+AGG - chr6_4:17185118-17185137 Msa0979850:CDS 20.0%
!!! AATTAGGTAAATATTTTCTG+GGG - chr6_4:17185134-17185153 Msa0979850:CDS 20.0%
! AGCCATATACATGTATATTA+TGG - chr6_4:17184249-17184268 Msa0979850:CDS 25.0%
! TGCCATAATATACATGTATA+TGG + chr6_4:17184254-17184273 None:intergenic 25.0%
! TATAAGAGAAAAAACACCTT+TGG - chr6_4:17184468-17184487 Msa0979850:CDS 25.0%
!! TTATACAATATCGAGCATTT+TGG + chr6_4:17184552-17184571 None:intergenic 25.0%
!! AAGATTTTATCCTTACTTCA+AGG - chr6_4:17184708-17184727 Msa0979850:intron 25.0%
! AAAATTAATTACTTACCGTG+GGG + chr6_4:17185052-17185071 None:intergenic 25.0%
!! ATTAGGTAAATATTTTCTGG+GGG - chr6_4:17185135-17185154 Msa0979850:CDS 25.0%
!! ATAACTCCTTTTCATTTTCA+GGG + chr6_4:17185249-17185268 None:intergenic 25.0%
!! AATAACTCCTTTTCATTTTC+AGG + chr6_4:17185250-17185269 None:intergenic 25.0%
! TCATTATTGTCTTCATGAAT+AGG + chr6_4:17185496-17185515 None:intergenic 25.0%
! CAGTTTTTGAATATGATGTG+TGG - chr6_4:17184301-17184320 Msa0979850:CDS 30.0%
ATAAATCTTGTATCCACCAA+AGG + chr6_4:17184487-17184506 None:intergenic 30.0%
!!! ACAAACTTTTAGGATTGCAT+TGG - chr6_4:17184818-17184837 Msa0979850:CDS 30.0%
ATTTAGCAACTTGGAATCAT+GGG + chr6_4:17184996-17185015 None:intergenic 30.0%
!! TGCTAAATGATGCTTTGAAA+AGG - chr6_4:17185008-17185027 Msa0979850:intron 30.0%
! TATCATCTACAAGATTTTGC+AGG - chr6_4:17185211-17185230 Msa0979850:CDS 30.0%
TAATGACCCTGAAAATGAAA+AGG - chr6_4:17185240-17185259 Msa0979850:CDS 30.0%
AATGTGGTTGAGAGAATATT+TGG - chr6_4:17185295-17185314 Msa0979850:CDS 30.0%
TGTGTCTTAAACAAAAATGG+AGG + chr6_4:17185358-17185377 None:intergenic 30.0%
GCTTGTGTCTTAAACAAAAA+TGG + chr6_4:17185361-17185380 None:intergenic 30.0%
ATGTTCAACTGGAAATTCAT+CGG + chr6_4:17185440-17185459 None:intergenic 30.0%
AATCTATGGAGGACTAATAT+AGG - chr6_4:17185559-17185578 Msa0979850:CDS 30.0%
CTAATATAGGTTCAGATATG+TGG - chr6_4:17185572-17185591 Msa0979850:CDS 30.0%
AATTCATGCTTTAATGCACG+TGG - chr6_4:17184273-17184292 Msa0979850:CDS 35.0%
CATAACTTTCGAAAGACATC+AGG + chr6_4:17184442-17184461 None:intergenic 35.0%
AAGAGAAAAAACACCTTTGG+TGG - chr6_4:17184471-17184490 Msa0979850:CDS 35.0%
AAGTGTGCCTGCAAAAATAA+GGG - chr6_4:17184678-17184697 Msa0979850:intron 35.0%
TGATCACATACCTTGAAGTA+AGG + chr6_4:17184721-17184740 None:intergenic 35.0%
AGCTGCTTGTAATTTCGATT+TGG - chr6_4:17184934-17184953 Msa0979850:intron 35.0%
CATTTAGCAACTTGGAATCA+TGG + chr6_4:17184997-17185016 None:intergenic 35.0%
! CAAAGCATCATTTAGCAACT+TGG + chr6_4:17185005-17185024 None:intergenic 35.0%
!! GATGCTTTGAAAAGGAAGAA+TGG - chr6_4:17185016-17185035 Msa0979850:intron 35.0%
!! AAATATTTTCTGGGGGATTG+CGG - chr6_4:17185142-17185161 Msa0979850:CDS 35.0%
!!! AAATTTTTAGCTCCACTTCG+AGG - chr6_4:17185181-17185200 Msa0979850:CDS 35.0%
AACGAGAAGATGCTAATCTA+TGG - chr6_4:17185545-17185564 Msa0979850:CDS 35.0%
GTAATTCGTCGTCGAGTTAA+TGG + chr6_4:17184343-17184362 None:intergenic 40.0%
TCGACGACGAATTACAAGTA+AGG - chr6_4:17184348-17184367 Msa0979850:CDS 40.0%
CGACGACGAATTACAAGTAA+GGG - chr6_4:17184349-17184368 Msa0979850:CDS 40.0%
CACTTGCAGCAAATTGTGAT+CGG + chr6_4:17184591-17184610 None:intergenic 40.0%
! CTCAATAGCACCTGATTTGA+TGG - chr6_4:17184642-17184661 Msa0979850:intron 40.0%
! GTTGGTTTAGCCATCAAATC+AGG + chr6_4:17184655-17184674 None:intergenic 40.0%
!! TATTTTTGCAGGCACACTTG+AGG + chr6_4:17184677-17184696 None:intergenic 40.0%
CAAGTGTGCCTGCAAAAATA+AGG - chr6_4:17184677-17184696 Msa0979850:intron 40.0%
!!! CTGCTTTCCCTTATTTTTGC+AGG + chr6_4:17184688-17184707 None:intergenic 40.0%
GATTGCATTGGAGCTATTGA+TGG - chr6_4:17184830-17184849 Msa0979850:CDS 40.0%
TGGTACACATATTCCTGCAT+TGG - chr6_4:17184850-17184869 Msa0979850:CDS 40.0%
! CATATTCCTGCATTGGTAAG+AGG - chr6_4:17184857-17184876 Msa0979850:CDS 40.0%
! CTACAAGATTTTGCAGGTCA+CGG - chr6_4:17185217-17185236 Msa0979850:CDS 40.0%
ATCTTCGTCATGCGTCTTTA+AGG - chr6_4:17185272-17185291 Msa0979850:CDS 40.0%
! TCATGCGTCTTTAAGGAATG+TGG - chr6_4:17185279-17185298 Msa0979850:CDS 40.0%
GACTCGTTAGAATGTTCAAC+TGG + chr6_4:17185451-17185470 None:intergenic 40.0%
GAGAAGATGCTAATCTATGG+AGG - chr6_4:17185548-17185567 Msa0979850:CDS 40.0%
GCGTCGTTCTTACAGATTGT+CGG - chr6_4:17184526-17184545 Msa0979850:CDS 45.0%
AGCAGTTATCGTGATCGTCA+TGG - chr6_4:17184890-17184909 Msa0979850:CDS 45.0%
GTTATCGTGATCGTCATGGA+AGG - chr6_4:17184894-17184913 Msa0979850:CDS 45.0%
GAGTCCATGTACGTTCTTAG+CGG - chr6_4:17184956-17184975 Msa0979850:intron 45.0%
AGTCCATGTACGTTCTTAGC+GGG - chr6_4:17184957-17184976 Msa0979850:intron 45.0%
!! AATGGACTAAAAGTGCCCCA+CGG - chr6_4:17185034-17185053 Msa0979850:intron 45.0%
GACACAAGCAGAACTTGTGT+TGG - chr6_4:17185372-17185391 Msa0979850:CDS 45.0%
TTTGCAGGCACACTTGAGGT+TGG + chr6_4:17184673-17184692 None:intergenic 50.0%
TCTCGTCCTCTTACCAATGC+AGG + chr6_4:17184866-17184885 None:intergenic 50.0%
! CATGTACGTTCTTAGCGGGT+GGG - chr6_4:17184961-17184980 Msa0979850:intron 50.0%
TGATATCGCACACCTCGAAG+TGG + chr6_4:17185196-17185215 None:intergenic 50.0%
CCACCCGCTAAGAACGTACA+TGG + chr6_4:17184963-17184982 None:intergenic 55.0%
CCATGTACGTTCTTAGCGGG+TGG - chr6_4:17184960-17184979 Msa0979850:intron 55.0%
! TACGTTCTTAGCGGGTGGGA+GGG - chr6_4:17184965-17184984 Msa0979850:intron 55.0%
! GTACGTTCTTAGCGGGTGGG+AGG - chr6_4:17184964-17184983 Msa0979850:intron 60.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 17184163 17185609 17184163 ID=Msa0979850;Name=Msa0979850
chr6_4 mRNA 17184163 17185609 17184163 ID=Msa0979850-mRNA-1;Parent=Msa0979850;Name=Msa0979850-mRNA-1;_AED=0.33;_eAED=0.39;_QI=0|0|0|0.66|1|1|3|0|420
chr6_4 exon 17185043 17185609 17185043 ID=Msa0979850-mRNA-1:exon:5477;Parent=Msa0979850-mRNA-1
chr6_4 exon 17184717 17184942 17184717 ID=Msa0979850-mRNA-1:exon:5476;Parent=Msa0979850-mRNA-1
chr6_4 exon 17184163 17184632 17184163 ID=Msa0979850-mRNA-1:exon:5475;Parent=Msa0979850-mRNA-1
chr6_4 CDS 17185043 17185609 17185043 ID=Msa0979850-mRNA-1:cds;Parent=Msa0979850-mRNA-1
chr6_4 CDS 17184717 17184942 17184717 ID=Msa0979850-mRNA-1:cds;Parent=Msa0979850-mRNA-1
chr6_4 CDS 17184163 17184632 17184163 ID=Msa0979850-mRNA-1:cds;Parent=Msa0979850-mRNA-1
Gene Sequence

>Msa0979850

ATGAGTGAAGATATATGTACTGAAGCTATAGATGATGATGATGATGATGATGCAAATGATGAAGAAGCAGATGAAGAATTTTATGAAGCCATATACATGTATATTATGGCAATTCATGCTTTAATGCACGTGGTAAATCAGTTTTTGAATATGATGTGTGGTGAACATGTTGAACGTCCATTAACTCGACGACGAATTACAAGTAAGGGATATGACTATATACATAAATCATTAAATGGAGATTCTGAAATCTTTCGACAAGTATATAGAATGTATCCTGATGTCTTTCGAAAGTTATGCATGATTATAAGAGAAAAAACACCTTTGGTGGATACAAGATTTATTTGTATTGGAGAAATGCTTGCGTCGTTCTTACAGATTGTCGGCCAAAATGCTCGATATTGTATAATTAGAAATACATTTGGCCGATCACAATTTGCTGCAAGTGAAAATTTTCACAAGATTTTGAAGGCTTTGAACTCAATAGCACCTGATTTGATGGCTAAACCAACCTCAAGTGTGCCTGCAAAAATAAGGGAAAGCAGAAGATTTTATCCTTACTTCAAGGATTGCATTGGAGCTATTGATGGTACACATATTCCTGCATTGGTAAGAGGACGAGATGTAAGCAGTTATCGTGATCGTCATGGAAGGATATCACAAAATGTATTAGCTGCTTGTAATTTCGATTTGGAGTCCATGTACGTTCTTAGCGGGTGGGAGGGTTCAGCCCATGATTCCAAGTTGCTAAATGATGCTTTGAAAAGGAAGAATGGACTAAAAGTGCCCCACGGTAAATATTTTCTGGGGGATTGCGGATTTGCTAATCGACGCAAATTTTTAGCTCCACTTCGAGGTGTGCGATATCATCTACAAGATTTTGCAGGTCACGGTAATGACCCTGAAAATGAAAAGGAGTTATTCAATCTTCGTCATGCGTCTTTAAGGAATGTGGTTGAGAGAATATTTGGTATTTTTAAATCGCGTTTCACAATTTTTAAGTCAGCACCTCCATTTTTGTTTAAGACACAAGCAGAACTTGTGTTGGCATGTGCAGCACTTCATAACTTTCTTCGCAAAGAATGTCGTTCCGATGAATTTCCAGTTGAACATTCTAACGAGTCTTCATCTTCATCTTCAATGTTACCTATTCATGAAGACAATAATGATGAACTCAATGTTCAAACACAAGAGCAAGAACGAGAAGATGCTAATCTATGGAGGACTAATATAGGTTCAGATATGTGGAGAGATGCTACTTAG

Protein sequence

>Msa0979850

MSEDICTEAIDDDDDDDANDEEADEEFYEAIYMYIMAIHALMHVVNQFLNMMCGEHVERPLTRRRITSKGYDYIHKSLNGDSEIFRQVYRMYPDVFRKLCMIIREKTPLVDTRFICIGEMLASFLQIVGQNARYCIIRNTFGRSQFAASENFHKILKALNSIAPDLMAKPTSSVPAKIRESRRFYPYFKDCIGAIDGTHIPALVRGRDVSSYRDRHGRISQNVLAACNFDLESMYVLSGWEGSAHDSKLLNDALKRKNGLKVPHGKYFLGDCGFANRRKFLAPLRGVRYHLQDFAGHGNDPENEKELFNLRHASLRNVVERIFGIFKSRFTIFKSAPPFLFKTQAELVLACAALHNFLRKECRSDEFPVEHSNESSSSSSMLPIHEDNNDELNVQTQEQEREDANLWRTNIGSDMWRDAT*