AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1408780


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1408780 MtrunA17_Chr8g0336901 66.176 136 36 3 28 155 1 134 2.38e-52 162
Msa1408780 MtrunA17_Chr8g0336891 67.742 124 36 3 13 134 101 222 7.68e-44 144
Msa1408780 MtrunA17_Chr8g0336921 43.590 117 61 4 16 130 37 150 2.84e-18 78.2
Msa1408780 MtrunA17_Chr7g0221241 34.058 138 81 4 8 144 34 162 9.82e-13 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTGATCAATTTGGCCTTA+TGG 0.174747 tig0023416:+31986 Msa1408780:CDS
TCTGATCAATTTGGCCTTAT+GGG 0.206934 tig0023416:+31987 Msa1408780:CDS
AACAAGAGTAAGAGTGAAAT+TGG 0.208263 tig0023416:-31913 None:intergenic
GAACATTGCTCTGATCAATT+TGG 0.249003 tig0023416:+31978 Msa1408780:CDS
TAAAGAAGTGTTTAAAGATT+TGG 0.260488 tig0023416:+31702 Msa1408780:CDS
ATTTCACTCTTACTCTTGTT+GGG 0.276326 tig0023416:+31916 Msa1408780:CDS
TTTCTCAGACAGTACTTTGC+TGG 0.310858 tig0023416:-32052 None:intergenic
AATTTCACTCTTACTCTTGT+TGG 0.319682 tig0023416:+31915 Msa1408780:CDS
GTTGTAGTTGCAAGAGAGTT+TGG 0.331524 tig0023416:-31873 None:intergenic
AAAATTCCTCCGCAACTTAG+AGG 0.340276 tig0023416:+32102 Msa1408780:CDS
TAGCCTTGACACTGATTTCA+AGG 0.344509 tig0023416:+31956 Msa1408780:CDS
GAAATTGGGAAAATAGTAAC+AGG 0.404325 tig0023416:-31898 None:intergenic
TTCTCAGACAGTACTTTGCT+GGG 0.420636 tig0023416:-32051 None:intergenic
GCAATGAAGTGCACAAATGA+TGG 0.482030 tig0023416:+31769 Msa1408780:CDS
TGTGAAAATAAGAACCCATA+AGG 0.488209 tig0023416:-32001 None:intergenic
GCAACTTAGAGGTAGATTGC+CGG 0.494987 tig0023416:+32113 Msa1408780:CDS
CCGATAGCAATGCGGTTTCA+AGG 0.497144 tig0023416:+31565 Msa1408780:CDS
TTTCACTCTTACTCTTGTTG+GGG 0.497838 tig0023416:+31917 Msa1408780:CDS
CCTTGAAACCGCATTGCTAT+CGG 0.508859 tig0023416:-31565 None:intergenic
ACAAGAGTAAGAGTGAAATT+GGG 0.519071 tig0023416:-31912 None:intergenic
AATCAGTGTCAAGGCTAGAA+AGG 0.525707 tig0023416:-31950 None:intergenic
CAATGAAGTGCACAAATGAT+GGG 0.531410 tig0023416:+31770 Msa1408780:CDS
GTTCACAACTTTAGCCGCAA+TGG 0.546781 tig0023416:+31744 Msa1408780:CDS
AGCAAGTGTCTACATCATCA+TGG 0.594333 tig0023416:+31828 Msa1408780:CDS
GTTCCTTGAAATCAGTGTCA+AGG 0.600166 tig0023416:-31959 None:intergenic
GGCAATCTACCTCTAAGTTG+CGG 0.630114 tig0023416:-32111 None:intergenic
GCACTTCATTGCAACCATTG+CGG 0.643939 tig0023416:-31758 None:intergenic
AATCTACCTCTAAGTTGCGG+AGG 0.644985 tig0023416:-32108 None:intergenic
AAGCATTCTACAATCTACAC+CGG 0.658127 tig0023416:-32132 None:intergenic
AATGAAGTGCACAAATGATG+GGG 0.734597 tig0023416:+31771 Msa1408780:CDS
TTGCAGCTCCGATAGCAATG+CGG 0.739864 tig0023416:+31557 Msa1408780:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAACACCTTGTT+TGG + tig0023416:31651-31670 Msa1408780:intron 20.0%
!!! AAAAAAAAAACACCTTGTTT+GGG + tig0023416:31652-31671 Msa1408780:intron 20.0%
!!! TAAAGAAGTGTTTAAAGATT+TGG + tig0023416:31702-31721 Msa1408780:CDS 20.0%
! GAAAATGAATATCATAGTTG+AGG - tig0023416:31606-31625 None:intergenic 25.0%
!!! AAATCAAACAGATTTTTGAG+AGG + tig0023416:32073-32092 Msa1408780:CDS 25.0%
GAAATTGGGAAAATAGTAAC+AGG - tig0023416:31901-31920 None:intergenic 30.0%
ACAAGAGTAAGAGTGAAATT+GGG - tig0023416:31915-31934 None:intergenic 30.0%
AACAAGAGTAAGAGTGAAAT+TGG - tig0023416:31916-31935 None:intergenic 30.0%
AATTTCACTCTTACTCTTGT+TGG + tig0023416:31915-31934 Msa1408780:CDS 30.0%
ATTTCACTCTTACTCTTGTT+GGG + tig0023416:31916-31935 Msa1408780:CDS 30.0%
TGTGAAAATAAGAACCCATA+AGG - tig0023416:32004-32023 None:intergenic 30.0%
CAATGAAGTGCACAAATGAT+GGG + tig0023416:31770-31789 Msa1408780:CDS 35.0%
AATGAAGTGCACAAATGATG+GGG + tig0023416:31771-31790 Msa1408780:CDS 35.0%
TTTCACTCTTACTCTTGTTG+GGG + tig0023416:31917-31936 Msa1408780:CDS 35.0%
GAACATTGCTCTGATCAATT+TGG + tig0023416:31978-31997 Msa1408780:CDS 35.0%
!! TCTGATCAATTTGGCCTTAT+GGG + tig0023416:31987-32006 Msa1408780:CDS 35.0%
ATGCATCAATCACCCAAACA+AGG - tig0023416:31667-31686 None:intergenic 40.0%
GTGATTGATGCATGTGAAAC+AGG + tig0023416:31674-31693 Msa1408780:intron 40.0%
GCAATGAAGTGCACAAATGA+TGG + tig0023416:31769-31788 Msa1408780:CDS 40.0%
AGCAAGTGTCTACATCATCA+TGG + tig0023416:31828-31847 Msa1408780:CDS 40.0%
GTTGTAGTTGCAAGAGAGTT+TGG - tig0023416:31876-31895 None:intergenic 40.0%
AATCAGTGTCAAGGCTAGAA+AGG - tig0023416:31953-31972 None:intergenic 40.0%
!! TAGCCTTGACACTGATTTCA+AGG + tig0023416:31956-31975 Msa1408780:CDS 40.0%
GTTCCTTGAAATCAGTGTCA+AGG - tig0023416:31962-31981 None:intergenic 40.0%
!! CTCTGATCAATTTGGCCTTA+TGG + tig0023416:31986-32005 Msa1408780:CDS 40.0%
TTCTCAGACAGTACTTTGCT+GGG - tig0023416:32054-32073 None:intergenic 40.0%
TTTCTCAGACAGTACTTTGC+TGG - tig0023416:32055-32074 None:intergenic 40.0%
AAAATTCCTCCGCAACTTAG+AGG + tig0023416:32102-32121 Msa1408780:CDS 40.0%
CCTTGAAACCGCATTGCTAT+CGG - tig0023416:31568-31587 None:intergenic 45.0%
GTTCACAACTTTAGCCGCAA+TGG + tig0023416:31744-31763 Msa1408780:CDS 45.0%
GCACTTCATTGCAACCATTG+CGG - tig0023416:31761-31780 None:intergenic 45.0%
AATCTACCTCTAAGTTGCGG+AGG - tig0023416:32111-32130 None:intergenic 45.0%
GGCAATCTACCTCTAAGTTG+CGG - tig0023416:32114-32133 None:intergenic 45.0%
GCAACTTAGAGGTAGATTGC+CGG + tig0023416:32113-32132 Msa1408780:CDS 45.0%
!!! TTTTTTTTTTTTTTTAGAAT+TGG - tig0023416:31640-31659 None:intergenic 5.0%
TTGCAGCTCCGATAGCAATG+CGG + tig0023416:31557-31576 Msa1408780:CDS 50.0%
CCGATAGCAATGCGGTTTCA+AGG + tig0023416:31565-31584 Msa1408780:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0023416 gene 31552 32140 31552 ID=Msa1408780;Name=Msa1408780
tig0023416 mRNA 31552 32140 31552 ID=Msa1408780-mRNA-1;Parent=Msa1408780;Name=Msa1408780-mRNA-1;_AED=0.52;_eAED=0.52;_QI=0|0|0|0.5|1|1|2|0|159
tig0023416 exon 31552 31586 31552 ID=Msa1408780-mRNA-1:exon:25702;Parent=Msa1408780-mRNA-1
tig0023416 exon 31696 32140 31696 ID=Msa1408780-mRNA-1:exon:25703;Parent=Msa1408780-mRNA-1
tig0023416 CDS 31552 31586 31552 ID=Msa1408780-mRNA-1:cds;Parent=Msa1408780-mRNA-1
tig0023416 CDS 31696 32140 31696 ID=Msa1408780-mRNA-1:cds;Parent=Msa1408780-mRNA-1
Gene Sequence

>Msa1408780

ATGGATTGCAGCTCCGATAGCAATGCGGTTTCAAGGAATAATAAAGAAGTGTTTAAAGATTTGGCAGAAATCTTTTATAAAATGTTCACAACTTTAGCCGCAATGGTTGCAATGAAGTGCACAAATGATGGGGATAAGCTATTAGCAAAAAACTATACTATATTTTCAGCAAGTGTCTACATCATCATGGCATACTTCTTAATTTGTGTGCTCCAAACTCTCTTGCAACTACAACATCCTGTTACTATTTTCCCAATTTCACTCTTACTCTTGTTGGGGTATGCTGTTTCCTTTCTAGCCTTGACACTGATTTCAAGGAACATTGCTCTGATCAATTTGGCCTTATGGGTTCTTATTTTCACATTTGTTGTCATCTTCAATAAATATCAACCCAGCAAAGTACTGTCTGAGAAAATCAAACAGATTTTTGAGAGGTATATCAAAATTCCTCCGCAACTTAGAGGTAGATTGCCGGTGTAG

Protein sequence

>Msa1408780

MDCSSDSNAVSRNNKEVFKDLAEIFYKMFTTLAAMVAMKCTNDGDKLLAKNYTIFSASVYIIMAYFLICVLQTLLQLQHPVTIFPISLLLLLGYAVSFLALTLISRNIALINLALWVLIFTFVVIFNKYQPSKVLSEKIKQIFERYIKIPPQLRGRLPV*