Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1310120 | A0A396GD97 | 66.176 | 136 | 36 | 3 | 28 | 155 | 1 | 134 | 1.23e-48 | 162 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015750 | Msa1310120 | 0.806350 | 8.582843e-50 | -8.615850e-47 |
| Msa0016570 | Msa1310120 | 0.812142 | 4.920022e-51 | -8.615850e-47 |
| Msa0092630 | Msa1310120 | 0.805695 | 1.178888e-49 | -8.615850e-47 |
| Msa0102070 | Msa1310120 | 0.837042 | 6.562775e-57 | -8.615850e-47 |
| Msa0636170 | Msa1310120 | 0.810242 | 1.270798e-50 | -8.615850e-47 |
| Msa0639610 | Msa1310120 | 0.810490 | 1.123339e-50 | -8.615850e-47 |
| Msa0689390 | Msa1310120 | 0.863640 | 2.155305e-64 | -8.615850e-47 |
| Msa0726140 | Msa1310120 | 0.825250 | 5.193113e-54 | -8.615850e-47 |
| Msa0729800 | Msa1310120 | 0.810318 | 1.223227e-50 | -8.615850e-47 |
| Msa0743810 | Msa1310120 | 0.868810 | 4.948815e-66 | -8.615850e-47 |
| Msa0745060 | Msa1310120 | 0.836691 | 8.065420e-57 | -8.615850e-47 |
| Msa0767720 | Msa1310120 | 0.826875 | 2.134270e-54 | -8.615850e-47 |
| Msa0783160 | Msa1310120 | 0.866920 | 2.002863e-65 | -8.615850e-47 |
| Msa0787600 | Msa1310120 | 0.842659 | 2.258587e-58 | -8.615850e-47 |
| Msa0808010 | Msa1310120 | 0.810254 | 1.263137e-50 | -8.615850e-47 |
| Msa0808020 | Msa1310120 | 0.805802 | 1.119309e-49 | -8.615850e-47 |
| Msa0811080 | Msa1310120 | 0.803986 | 2.682687e-49 | -8.615850e-47 |
| Msa0811270 | Msa1310120 | 0.802358 | 5.827822e-49 | -8.615850e-47 |
| Msa0825860 | Msa1310120 | 0.844637 | 6.683676e-59 | -8.615850e-47 |
| Msa0142220 | Msa1310120 | 0.825262 | 5.158827e-54 | -8.615850e-47 |
| Msa0145750 | Msa1310120 | 0.800258 | 1.567928e-48 | -8.615850e-47 |
| Msa0153910 | Msa1310120 | 0.831909 | 1.277761e-55 | -8.615850e-47 |
| Msa0227480 | Msa1310120 | 0.844643 | 6.658667e-59 | -8.615850e-47 |
| Msa0232850 | Msa1310120 | 0.813431 | 2.569058e-51 | -8.615850e-47 |
| Msa0235140 | Msa1310120 | 0.801210 | 1.002744e-48 | -8.615850e-47 |
| Msa1230430 | Msa1310120 | 0.824130 | 9.536920e-54 | -8.615850e-47 |
| Msa1269670 | Msa1310120 | 0.834644 | 2.660846e-56 | -8.615850e-47 |
| Msa1270230 | Msa1310120 | 0.842048 | 3.279867e-58 | -8.615850e-47 |
| Msa1270240 | Msa1310120 | 0.822591 | 2.182899e-53 | -8.615850e-47 |
| Msa1270270 | Msa1310120 | 0.827466 | 1.540773e-54 | -8.615850e-47 |
| Msa1273230 | Msa1310120 | 0.819513 | 1.116566e-52 | -8.615850e-47 |
| Msa1283330 | Msa1310120 | 0.857712 | 1.353977e-62 | -8.615850e-47 |
| Msa1285930 | Msa1310120 | 0.888653 | 4.879569e-73 | -8.615850e-47 |
| Msa1287800 | Msa1310120 | 0.856817 | 2.489799e-62 | -8.615850e-47 |
| Msa1287810 | Msa1310120 | 0.868278 | 7.352599e-66 | -8.615850e-47 |
| Msa1287830 | Msa1310120 | 0.808608 | 2.848441e-50 | -8.615850e-47 |
| Msa1289430 | Msa1310120 | 0.826451 | 2.692993e-54 | -8.615850e-47 |
| Msa1297210 | Msa1310120 | 0.822700 | 2.058535e-53 | -8.615850e-47 |
| Msa1307890 | Msa1310120 | 0.823226 | 1.552333e-53 | -8.615850e-47 |
| Msa1310120 | Msa1369430 | 0.806961 | 6.379528e-50 | -8.615850e-47 |
| Msa1310120 | Msa1397150 | 0.806221 | 9.140590e-50 | -8.615850e-47 |
| Msa1310120 | Msa1397580 | 0.800116 | 1.676202e-48 | -8.615850e-47 |
| Msa1310120 | Msa1402790 | 0.817567 | 3.084777e-52 | -8.615850e-47 |
| Msa1310120 | Msa1404000 | 0.823681 | 1.215188e-53 | -8.615850e-47 |
| Msa1310120 | Msa1408780 | 0.965738 | 5.376025e-125 | -8.615850e-47 |
| Msa1310120 | Msa1415310 | 0.812445 | 4.225450e-51 | -8.615850e-47 |
| Msa1310120 | Msa1422180 | 0.827255 | 1.730983e-54 | -8.615850e-47 |
| Msa1310120 | Msa1433120 | 0.816852 | 4.465691e-52 | -8.615850e-47 |
| Msa0384800 | Msa1310120 | 0.829281 | 5.619456e-55 | -8.615850e-47 |
| Msa0414410 | Msa1310120 | 0.816796 | 4.596769e-52 | -8.615850e-47 |
| Msa0414420 | Msa1310120 | 0.843875 | 1.070565e-58 | -8.615850e-47 |
| Msa0435230 | Msa1310120 | 0.800703 | 1.272717e-48 | -8.615850e-47 |
| Msa0436780 | Msa1310120 | 0.827150 | 1.833720e-54 | -8.615850e-47 |
| Msa0438620 | Msa1310120 | 0.806440 | 8.215888e-50 | -8.615850e-47 |
| Msa0452900 | Msa1310120 | 0.857319 | 1.769652e-62 | -8.615850e-47 |
| Msa0453120 | Msa1310120 | 0.814366 | 1.597771e-51 | -8.615850e-47 |
| Msa0454910 | Msa1310120 | 0.813957 | 1.967500e-51 | -8.615850e-47 |
| Msa0979850 | Msa1310120 | 0.809581 | 1.763074e-50 | -8.615850e-47 |
| Msa0981270 | Msa1310120 | 0.801707 | 7.929692e-49 | -8.615850e-47 |
| Msa0989060 | Msa1310120 | 0.854455 | 1.217077e-61 | -8.615850e-47 |
| Msa1016280 | Msa1310120 | 0.860874 | 1.523456e-63 | -8.615850e-47 |
| Msa1023050 | Msa1310120 | 0.802218 | 6.227498e-49 | -8.615850e-47 |
| Msa1024240 | Msa1310120 | 0.807674 | 4.503756e-50 | -8.615850e-47 |
| Msa1025360 | Msa1310120 | 0.868064 | 8.618276e-66 | -8.615850e-47 |
| Msa1025370 | Msa1310120 | 0.853150 | 2.889436e-61 | -8.615850e-47 |
| Msa1044830 | Msa1310120 | 0.857601 | 1.460866e-62 | -8.615850e-47 |
| Msa1044840 | Msa1310120 | 0.867796 | 1.050963e-65 | -8.615850e-47 |
| Msa1060840 | Msa1310120 | 0.829622 | 4.644220e-55 | -8.615850e-47 |
| Msa1087490 | Msa1310120 | 0.899183 | 2.549350e-77 | -8.615850e-47 |
| Msa1092990 | Msa1310120 | 0.832647 | 8.390413e-56 | -8.615850e-47 |
| Msa1100980 | Msa1310120 | 0.866674 | 2.399409e-65 | -8.615850e-47 |
| Msa0251320 | Msa1310120 | 0.803334 | 3.663240e-49 | -8.615850e-47 |
| Msa0267000 | Msa1310120 | 0.854806 | 9.628935e-62 | -8.615850e-47 |
| Msa0267010 | Msa1310120 | 0.864222 | 1.420618e-64 | -8.615850e-47 |
| Msa0270520 | Msa1310120 | 0.806106 | 9.664649e-50 | -8.615850e-47 |
| Msa0304170 | Msa1310120 | 0.806362 | 8.536662e-50 | -8.615850e-47 |
| Msa0306460 | Msa1310120 | 0.801631 | 8.221420e-49 | -8.615850e-47 |
| Msa0342240 | Msa1310120 | 0.855482 | 6.125660e-62 | -8.615850e-47 |
| Msa0360510 | Msa1310120 | 0.825197 | 5.345098e-54 | -8.615850e-47 |
| Msa1109440 | Msa1310120 | 0.853903 | 1.756253e-61 | -8.615850e-47 |
| Msa1121200 | Msa1310120 | 0.810961 | 8.885558e-51 | -8.615850e-47 |
| Msa1135220 | Msa1310120 | 0.803462 | 3.447002e-49 | -8.615850e-47 |
| Msa1135740 | Msa1310120 | 0.846819 | 1.709337e-59 | -8.615850e-47 |
| Msa1146110 | Msa1310120 | 0.828311 | 9.649792e-55 | -8.615850e-47 |
| Msa1149360 | Msa1310120 | 0.816250 | 6.091810e-52 | -8.615850e-47 |
| Msa1150370 | Msa1310120 | 0.832163 | 1.105909e-55 | -8.615850e-47 |
| Msa1173920 | Msa1310120 | 0.805191 | 1.503439e-49 | -8.615850e-47 |
| Msa1182090 | Msa1310120 | 0.816059 | 6.721673e-52 | -8.615850e-47 |
| Msa1187940 | Msa1310120 | 0.826927 | 2.073672e-54 | -8.615850e-47 |
| Msa1187950 | Msa1310120 | 0.831233 | 1.875137e-55 | -8.615850e-47 |
| Msa1192640 | Msa1310120 | 0.868218 | 7.689143e-66 | -8.615850e-47 |
| Msa1211030 | Msa1310120 | 0.860752 | 1.659196e-63 | -8.615850e-47 |
| Msa0485030 | Msa1310120 | 0.802514 | 5.412401e-49 | -8.615850e-47 |
| Msa0564890 | Msa1310120 | 0.845359 | 4.266173e-59 | -8.615850e-47 |
| Msa0846350 | Msa1310120 | 0.804261 | 2.351257e-49 | -8.615850e-47 |
| Msa0848960 | Msa1310120 | 0.801303 | 9.597037e-49 | -8.615850e-47 |
| Msa0865320 | Msa1310120 | 0.829430 | 5.170937e-55 | -8.615850e-47 |
| Msa0872230 | Msa1310120 | 0.805742 | 1.152439e-49 | -8.615850e-47 |
| Msa0885290 | Msa1310120 | 0.814093 | 1.836575e-51 | -8.615850e-47 |
| Msa0907260 | Msa1310120 | 0.828497 | 8.698935e-55 | -8.615850e-47 |
| Msa0956270 | Msa1310120 | 0.833532 | 5.052525e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1310120 | MtrunA17_Chr8g0336901 | 66.176 | 136 | 36 | 3 | 28 | 155 | 1 | 134 | 2.38e-52 | 162 |
| Msa1310120 | MtrunA17_Chr8g0336891 | 67.742 | 124 | 36 | 3 | 13 | 134 | 101 | 222 | 7.68e-44 | 144 |
| Msa1310120 | MtrunA17_Chr8g0336921 | 43.590 | 117 | 61 | 4 | 16 | 130 | 37 | 150 | 2.84e-18 | 78.2 |
| Msa1310120 | MtrunA17_Chr7g0221241 | 34.058 | 138 | 81 | 4 | 8 | 144 | 34 | 162 | 9.82e-13 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTGATCAATTTGGCCTTA+TGG | 0.174747 | 8_4:+6663821 | Msa1310120:CDS |
| TCTGATCAATTTGGCCTTAT+GGG | 0.206934 | 8_4:+6663822 | Msa1310120:CDS |
| AACAAGAGTAAGAGTGAAAT+TGG | 0.208263 | 8_4:-6663748 | Msa1310130:CDS |
| GAACATTGCTCTGATCAATT+TGG | 0.249003 | 8_4:+6663813 | Msa1310120:CDS |
| TAAAGAAGTGTTTAAAGATT+TGG | 0.260488 | 8_4:+6663537 | Msa1310120:CDS |
| ATTTCACTCTTACTCTTGTT+GGG | 0.276326 | 8_4:+6663751 | Msa1310120:CDS |
| TTTCTCAGACAGTACTTTGC+TGG | 0.310858 | 8_4:-6663887 | None:intergenic |
| AATTTCACTCTTACTCTTGT+TGG | 0.319682 | 8_4:+6663750 | Msa1310120:CDS |
| GTTGTAGTTGCAAGAGAGTT+TGG | 0.331524 | 8_4:-6663708 | Msa1310130:CDS |
| AAAATTCCTCCGCAACTTAG+AGG | 0.340276 | 8_4:+6663937 | Msa1310120:CDS |
| TAGCCTTGACACTGATTTCA+AGG | 0.344509 | 8_4:+6663791 | Msa1310120:CDS |
| GAAATTGGGAAAATAGTAAC+AGG | 0.404325 | 8_4:-6663733 | Msa1310130:CDS |
| TTCTCAGACAGTACTTTGCT+GGG | 0.420636 | 8_4:-6663886 | None:intergenic |
| GCAATGAAGTGCACAAATGA+TGG | 0.482030 | 8_4:+6663604 | Msa1310120:CDS |
| TGTGAAAATAAGAACCCATA+AGG | 0.488209 | 8_4:-6663836 | Msa1310130:CDS |
| GCAACTTAGAGGTAGATTGC+CGG | 0.494987 | 8_4:+6663948 | Msa1310120:CDS |
| CCGATAGCAATGCGGTTTCA+AGG | 0.497144 | 8_4:+6663400 | Msa1310120:CDS |
| TTTCACTCTTACTCTTGTTG+GGG | 0.497838 | 8_4:+6663752 | Msa1310120:CDS |
| CCTTGAAACCGCATTGCTAT+CGG | 0.508859 | 8_4:-6663400 | None:intergenic |
| ACAAGAGTAAGAGTGAAATT+GGG | 0.519071 | 8_4:-6663747 | Msa1310130:CDS |
| AATCAGTGTCAAGGCTAGAA+AGG | 0.525707 | 8_4:-6663785 | Msa1310130:CDS |
| CAATGAAGTGCACAAATGAT+GGG | 0.531410 | 8_4:+6663605 | Msa1310120:CDS |
| GTTCACAACTTTAGCCGCAA+TGG | 0.546781 | 8_4:+6663579 | Msa1310120:CDS |
| AGCAAGTGTCTACATCATCA+TGG | 0.594333 | 8_4:+6663663 | Msa1310120:CDS |
| GTTCCTTGAAATCAGTGTCA+AGG | 0.600166 | 8_4:-6663794 | Msa1310130:CDS |
| GGCAATCTACCTCTAAGTTG+CGG | 0.630114 | 8_4:-6663946 | None:intergenic |
| GCACTTCATTGCAACCATTG+CGG | 0.643939 | 8_4:-6663593 | Msa1310130:CDS |
| AATCTACCTCTAAGTTGCGG+AGG | 0.644985 | 8_4:-6663943 | None:intergenic |
| AAGCATTCTACAATCTACAC+CGG | 0.658127 | 8_4:-6663967 | None:intergenic |
| AATGAAGTGCACAAATGATG+GGG | 0.734597 | 8_4:+6663606 | Msa1310120:CDS |
| TTGCAGCTCCGATAGCAATG+CGG | 0.739864 | 8_4:+6663392 | Msa1310120:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAACACCTTGTT+TGG | + | chr8_4:6663486-6663505 | Msa1310120:intron | 20.0% |
| !!! | AAAAAAAAAACACCTTGTTT+GGG | + | chr8_4:6663487-6663506 | Msa1310120:intron | 20.0% |
| !!! | TAAAGAAGTGTTTAAAGATT+TGG | + | chr8_4:6663537-6663556 | Msa1310120:CDS | 20.0% |
| ! | GAAAATGAATATCATAGTTG+AGG | - | chr8_4:6663441-6663460 | None:intergenic | 25.0% |
| !!! | AAATCAAACAGATTTTTGAG+AGG | + | chr8_4:6663908-6663927 | Msa1310120:CDS | 25.0% |
| GAAATTGGGAAAATAGTAAC+AGG | - | chr8_4:6663736-6663755 | Msa1310130:CDS | 30.0% | |
| ACAAGAGTAAGAGTGAAATT+GGG | - | chr8_4:6663750-6663769 | Msa1310130:CDS | 30.0% | |
| AACAAGAGTAAGAGTGAAAT+TGG | - | chr8_4:6663751-6663770 | Msa1310130:CDS | 30.0% | |
| AATTTCACTCTTACTCTTGT+TGG | + | chr8_4:6663750-6663769 | Msa1310120:CDS | 30.0% | |
| ATTTCACTCTTACTCTTGTT+GGG | + | chr8_4:6663751-6663770 | Msa1310120:CDS | 30.0% | |
| TGTGAAAATAAGAACCCATA+AGG | - | chr8_4:6663839-6663858 | Msa1310130:CDS | 30.0% | |
| CAATGAAGTGCACAAATGAT+GGG | + | chr8_4:6663605-6663624 | Msa1310120:CDS | 35.0% | |
| AATGAAGTGCACAAATGATG+GGG | + | chr8_4:6663606-6663625 | Msa1310120:CDS | 35.0% | |
| TTTCACTCTTACTCTTGTTG+GGG | + | chr8_4:6663752-6663771 | Msa1310120:CDS | 35.0% | |
| GAACATTGCTCTGATCAATT+TGG | + | chr8_4:6663813-6663832 | Msa1310120:CDS | 35.0% | |
| !! | TCTGATCAATTTGGCCTTAT+GGG | + | chr8_4:6663822-6663841 | Msa1310120:CDS | 35.0% |
| ATGCATCAATCACCCAAACA+AGG | - | chr8_4:6663502-6663521 | None:intergenic | 40.0% | |
| GTGATTGATGCATGTGAAAC+AGG | + | chr8_4:6663509-6663528 | Msa1310120:intron | 40.0% | |
| GCAATGAAGTGCACAAATGA+TGG | + | chr8_4:6663604-6663623 | Msa1310120:CDS | 40.0% | |
| AGCAAGTGTCTACATCATCA+TGG | + | chr8_4:6663663-6663682 | Msa1310120:CDS | 40.0% | |
| GTTGTAGTTGCAAGAGAGTT+TGG | - | chr8_4:6663711-6663730 | Msa1310130:CDS | 40.0% | |
| AATCAGTGTCAAGGCTAGAA+AGG | - | chr8_4:6663788-6663807 | Msa1310130:CDS | 40.0% | |
| !! | TAGCCTTGACACTGATTTCA+AGG | + | chr8_4:6663791-6663810 | Msa1310120:CDS | 40.0% |
| GTTCCTTGAAATCAGTGTCA+AGG | - | chr8_4:6663797-6663816 | Msa1310130:CDS | 40.0% | |
| !! | CTCTGATCAATTTGGCCTTA+TGG | + | chr8_4:6663821-6663840 | Msa1310120:CDS | 40.0% |
| TTCTCAGACAGTACTTTGCT+GGG | - | chr8_4:6663889-6663908 | None:intergenic | 40.0% | |
| TTTCTCAGACAGTACTTTGC+TGG | - | chr8_4:6663890-6663909 | None:intergenic | 40.0% | |
| AAAATTCCTCCGCAACTTAG+AGG | + | chr8_4:6663937-6663956 | Msa1310120:CDS | 40.0% | |
| CCTTGAAACCGCATTGCTAT+CGG | - | chr8_4:6663403-6663422 | None:intergenic | 45.0% | |
| GTTCACAACTTTAGCCGCAA+TGG | + | chr8_4:6663579-6663598 | Msa1310120:CDS | 45.0% | |
| GCACTTCATTGCAACCATTG+CGG | - | chr8_4:6663596-6663615 | Msa1310130:CDS | 45.0% | |
| AATCTACCTCTAAGTTGCGG+AGG | - | chr8_4:6663946-6663965 | None:intergenic | 45.0% | |
| GGCAATCTACCTCTAAGTTG+CGG | - | chr8_4:6663949-6663968 | None:intergenic | 45.0% | |
| GCAACTTAGAGGTAGATTGC+CGG | + | chr8_4:6663948-6663967 | Msa1310120:CDS | 45.0% | |
| !!! | TTTTTTTTTTTTTTTAGAAT+TGG | - | chr8_4:6663475-6663494 | None:intergenic | 5.0% |
| TTGCAGCTCCGATAGCAATG+CGG | + | chr8_4:6663392-6663411 | Msa1310120:CDS | 50.0% | |
| CCGATAGCAATGCGGTTTCA+AGG | + | chr8_4:6663400-6663419 | Msa1310120:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 6663387 | 6663975 | 6663387 | ID=Msa1310120;Name=Msa1310120 |
| chr8_4 | mRNA | 6663387 | 6663975 | 6663387 | ID=Msa1310120-mRNA-1;Parent=Msa1310120;Name=Msa1310120-mRNA-1;_AED=0.52;_eAED=0.52;_QI=0|0|0|0.5|1|1|2|0|159 |
| chr8_4 | exon | 6663387 | 6663421 | 6663387 | ID=Msa1310120-mRNA-1:exon:2246;Parent=Msa1310120-mRNA-1 |
| chr8_4 | exon | 6663531 | 6663975 | 6663531 | ID=Msa1310120-mRNA-1:exon:2247;Parent=Msa1310120-mRNA-1 |
| chr8_4 | CDS | 6663387 | 6663421 | 6663387 | ID=Msa1310120-mRNA-1:cds;Parent=Msa1310120-mRNA-1 |
| chr8_4 | CDS | 6663531 | 6663975 | 6663531 | ID=Msa1310120-mRNA-1:cds;Parent=Msa1310120-mRNA-1 |
| Gene Sequence |
| Protein sequence |
