AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0833300


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833300 MtrunA17_Chr5g0398621 90.722 97 8 1 2 98 152 247 3.81e-57 176
Msa0833300 MtrunA17_Chr5g0398591 65.000 100 23 3 1 96 9 100 2.20e-33 112
Msa0833300 MtrunA17_Chr5g0398601 68.000 75 18 2 25 95 4 76 8.75e-22 88.2
Msa0833300 MtrunA17_Chr7g0245841 54.455 101 38 1 3 95 179 279 2.73e-17 74.7
Msa0833300 MtrunA17_Chr1g0179461 39.326 89 52 1 3 89 198 286 8.30e-16 70.9
Msa0833300 MtrunA17_Chr8g0351761 59.211 76 30 1 3 77 194 269 1.65e-15 70.1
Msa0833300 MtrunA17_Chr7g0247431 40.000 80 48 0 3 82 209 288 4.72e-15 68.6
Msa0833300 MtrunA17_Chr1g0188211 54.118 85 38 1 3 86 185 269 3.03e-14 66.2
Msa0833300 MtrunA17_Chr6g0476061 42.222 90 48 1 5 94 184 269 1.57e-13 64.3
Msa0833300 MtrunA17_Chr1g0190011 46.479 71 34 2 2 71 161 228 7.84e-13 62.4
Msa0833300 MtrunA17_Chr5g0397551 47.761 67 34 1 1 67 184 249 3.04e-12 60.8
Msa0833300 MtrunA17_Chr4g0056761 53.933 89 40 1 3 90 196 284 5.19e-12 60.1
Msa0833300 MtrunA17_Chr6g0465361 37.209 86 51 2 6 89 18 102 1.28e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833300 AT4G12080.1 51.042 96 43 2 3 94 222 317 1.18e-21 87.4
Msa0833300 AT2G33620.1 43.689 103 45 1 3 92 215 317 2.66e-18 78.2
Msa0833300 AT2G33620.4 43.689 103 45 1 3 92 215 317 2.66e-18 78.2
Msa0833300 AT2G33620.2 43.689 103 45 1 3 92 215 317 2.66e-18 78.2
Msa0833300 AT2G33620.3 43.689 103 45 1 3 92 215 317 2.66e-18 78.2
Msa0833300 AT5G62260.1 48.052 77 40 0 3 79 212 288 2.51e-17 75.5
Msa0833300 AT5G62260.3 48.052 77 40 0 3 79 212 288 2.51e-17 75.5
Msa0833300 AT4G22770.2 52.174 92 43 1 3 93 202 293 6.51e-15 68.6
Msa0833300 AT4G22770.1 52.174 92 43 1 3 93 202 293 6.51e-15 68.6
Msa0833300 AT5G51590.1 50.769 65 32 0 3 67 230 294 1.99e-14 67.4
Msa0833300 AT4G17950.1 42.708 96 52 2 3 95 275 370 2.71e-14 67.0
Msa0833300 AT4G25320.1 44.444 72 40 0 3 74 218 289 4.08e-14 66.6
Msa0833300 AT4G00200.2 50.562 89 43 1 3 91 175 262 2.69e-13 63.9
Msa0833300 AT4G00200.1 50.562 89 43 1 3 91 175 262 2.69e-13 63.9
Msa0833300 AT5G62260.2 44.615 65 36 0 3 67 212 276 9.78e-13 62.4
Msa0833300 AT2G45850.1 41.250 80 44 1 3 82 214 290 1.23e-12 62.4
Msa0833300 AT2G45850.2 41.250 80 44 1 3 82 214 290 1.23e-12 62.4

Find 25 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATAGGCTGTTATGTATT+TGG 0.193670 5_4:-6483654 None:intergenic
TATGAAATTCGAACTTTATC+TGG 0.233802 5_4:+6483149 Msa0833300:CDS
AATAATGCAAATTGAATTTC+AGG 0.249584 5_4:+6483121 None:intergenic
GACCAGGTACTTACATTTAC+AGG 0.268526 5_4:-6483296 None:intergenic
CGCGGAAGGCGGAGTATATC+TGG 0.344559 5_4:+6483194 Msa0833300:CDS
TGGCATGAGTGTTTCTTTAG+TGG 0.383981 5_4:+6483217 Msa0833300:CDS
TTCTGCTCATGTTGTTCACT+TGG 0.400794 5_4:-6483570 None:intergenic
GCAGTAGACATGGGAGCAGC+TGG 0.426144 5_4:-6483627 None:intergenic
GTTGTTGGCGGTCGTGTAGC+TGG 0.449009 5_4:+6483254 Msa0833300:CDS
GCTGCTCCCATGTCTACTGC+TGG 0.473409 5_4:+6483630 Msa0833300:CDS
ATCTGGTTTGTTCATGCCAG+AGG 0.521232 5_4:+6483166 Msa0833300:CDS
GTTTCTTTAGTGGATCTACA+TGG 0.535434 5_4:+6483227 Msa0833300:CDS
TGCCAGAGGAAAAGCGCGGA+AGG 0.539759 5_4:+6483180 Msa0833300:CDS
ACAGGACTTGCAGCCACCAA+TGG 0.541015 5_4:-6483278 None:intergenic
ATAATGCAAATTGAATTTCA+GGG 0.542355 5_4:+6483122 None:intergenic
CGGTCGTGTAGCTGGTCCAT+TGG 0.545745 5_4:+6483262 Msa0833300:CDS
TCGTGTAGCTGGTCCATTGG+TGG 0.554685 5_4:+6483265 Msa0833300:CDS
GATCTACATGGACATGTTGT+TGG 0.565136 5_4:+6483239 Msa0833300:CDS
ATTTGGACCAGCAGTAGACA+TGG 0.578513 5_4:-6483637 None:intergenic
CTACATGGACATGTTGTTGG+CGG 0.596833 5_4:+6483242 Msa0833300:CDS
GATATGTATCAGGTAGTGGT+AGG 0.603294 5_4:+6483537 Msa0833300:intron
CAGAGGAAAAGCGCGGAAGG+CGG 0.629538 5_4:+6483183 Msa0833300:CDS
TTTGGACCAGCAGTAGACAT+GGG 0.688042 5_4:-6483636 None:intergenic
GGAAGGCGGAGTATATCTGG+TGG 0.708998 5_4:+6483197 Msa0833300:CDS
TTCATGCCAGAGGAAAAGCG+CGG 0.748400 5_4:+6483176 Msa0833300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATGAAATTCGAACTTTATC+TGG + chr5_4:6483149-6483168 Msa0833300:CDS 25.0%
! GCTTATGTAGTTATTTATCT+TGG + chr5_4:6483365-6483384 Msa0833300:intron 25.0%
! ATGCAATTCTGATAATTTAG+TGG + chr5_4:6483459-6483478 Msa0833300:intron 25.0%
GCTAAAAACACAGAATAATG+AGG + chr5_4:6483593-6483612 Msa0833300:CDS 30.0%
! GTTTCTTTAGTGGATCTACA+TGG + chr5_4:6483227-6483246 Msa0833300:CDS 35.0%
TCTGTTTCCTGCTAAAGAAA+TGG + chr5_4:6483418-6483437 Msa0833300:intron 35.0%
! ATAGTTGCCATTTCTTTAGC+AGG - chr5_4:6483428-6483447 None:intergenic 35.0%
ATTCTCATCACTGTTTCCAA+TGG + chr5_4:6483511-6483530 Msa0833300:intron 35.0%
CCTGATACATATCAATCCAT+TGG - chr5_4:6483530-6483549 None:intergenic 35.0%
!! CCAATGGATTGATATGTATC+AGG + chr5_4:6483527-6483546 Msa0833300:intron 35.0%
GATTGATATGTATCAGGTAG+TGG + chr5_4:6483533-6483552 Msa0833300:intron 35.0%
TGGCATGAGTGTTTCTTTAG+TGG + chr5_4:6483217-6483236 Msa0833300:CDS 40.0%
GATCTACATGGACATGTTGT+TGG + chr5_4:6483239-6483258 Msa0833300:CDS 40.0%
GTCCTGTAAATGTAAGTACC+TGG + chr5_4:6483294-6483313 Msa0833300:intron 40.0%
GACCAGGTACTTACATTTAC+AGG - chr5_4:6483299-6483318 None:intergenic 40.0%
TAAATGTAAGTACCTGGTCC+TGG + chr5_4:6483300-6483319 Msa0833300:intron 40.0%
AAAAGACATATTCCCTAGCC+AGG - chr5_4:6483321-6483340 None:intergenic 40.0%
TTCGCAAACTAAAGTCTAGC+AGG + chr5_4:6483340-6483359 Msa0833300:intron 40.0%
TCGCAAACTAAAGTCTAGCA+GGG + chr5_4:6483341-6483360 Msa0833300:intron 40.0%
! GATATGTATCAGGTAGTGGT+AGG + chr5_4:6483537-6483556 Msa0833300:intron 40.0%
TTCTGCTCATGTTGTTCACT+TGG - chr5_4:6483573-6483592 None:intergenic 40.0%
!! ATCTGGTTTGTTCATGCCAG+AGG + chr5_4:6483166-6483185 Msa0833300:CDS 45.0%
CTACATGGACATGTTGTTGG+CGG + chr5_4:6483242-6483261 Msa0833300:CDS 45.0%
TTTGGACCAGCAGTAGACAT+GGG - chr5_4:6483639-6483658 None:intergenic 45.0%
ATTTGGACCAGCAGTAGACA+TGG - chr5_4:6483640-6483659 None:intergenic 45.0%
TTCATGCCAGAGGAAAAGCG+CGG + chr5_4:6483176-6483195 Msa0833300:CDS 50.0%
TAAGTACCTGGTCCTGGCTA+GGG + chr5_4:6483306-6483325 Msa0833300:intron 50.0%
GGAAGGCGGAGTATATCTGG+TGG + chr5_4:6483197-6483216 Msa0833300:CDS 55.0%
TCGTGTAGCTGGTCCATTGG+TGG + chr5_4:6483265-6483284 Msa0833300:CDS 55.0%
ACAGGACTTGCAGCCACCAA+TGG - chr5_4:6483281-6483300 None:intergenic 55.0%
GTAAGTACCTGGTCCTGGCT+AGG + chr5_4:6483305-6483324 Msa0833300:intron 55.0%
CATATTCCCTAGCCAGGACC+AGG - chr5_4:6483315-6483334 None:intergenic 55.0%
! TGCCAGAGGAAAAGCGCGGA+AGG + chr5_4:6483180-6483199 Msa0833300:CDS 60.0%
! CAGAGGAAAAGCGCGGAAGG+CGG + chr5_4:6483183-6483202 Msa0833300:CDS 60.0%
CGCGGAAGGCGGAGTATATC+TGG + chr5_4:6483194-6483213 Msa0833300:CDS 60.0%
GTTGTTGGCGGTCGTGTAGC+TGG + chr5_4:6483254-6483273 Msa0833300:CDS 60.0%
CGGTCGTGTAGCTGGTCCAT+TGG + chr5_4:6483262-6483281 Msa0833300:CDS 60.0%
GCAGTAGACATGGGAGCAGC+TGG - chr5_4:6483630-6483649 None:intergenic 60.0%
GCTGCTCCCATGTCTACTGC+TGG + chr5_4:6483630-6483649 Msa0833300:CDS 60.0%
! CGCCTTCCGCGCTTTTCCTC+TGG - chr5_4:6483185-6483204 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 6483125 6483665 6483125 ID=Msa0833300;Name=Msa0833300
chr5_4 mRNA 6483125 6483665 6483125 ID=Msa0833300-mRNA-1;Parent=Msa0833300;Name=Msa0833300-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|1|1|2|0|98
chr5_4 exon 6483125 6483304 6483125 ID=Msa0833300-mRNA-1:exon:2815;Parent=Msa0833300-mRNA-1
chr5_4 exon 6483549 6483665 6483549 ID=Msa0833300-mRNA-1:exon:2816;Parent=Msa0833300-mRNA-1
chr5_4 CDS 6483125 6483304 6483125 ID=Msa0833300-mRNA-1:cds;Parent=Msa0833300-mRNA-1
chr5_4 CDS 6483549 6483665 6483549 ID=Msa0833300-mRNA-1:cds;Parent=Msa0833300-mRNA-1
Gene Sequence

>Msa0833300

ATGCAAATTGAATTTCAGGGTAGATATGAAATTCGAACTTTATCTGGTTTGTTCATGCCAGAGGAAAAGCGCGGAAGGCGGAGTATATCTGGTGGCATGAGTGTTTCTTTAGTGGATCTACATGGACATGTTGTTGGCGGTCGTGTAGCTGGTCCATTGGTGGCTGCAAGTCCTGTAAATGTAGTGGTAGGCAGTTTTCTGCCAAGTGAACAACATGAGCAGAAGCTAAAAACACAGAATAATGAGGTAATTTCAACTCCAGCTGCTCCCATGTCTACTGCTGGTCCAAATACATAA

Protein sequence

>Msa0833300

MQIEFQGRYEIRTLSGLFMPEEKRGRRSISGGMSVSLVDLHGHVVGGRVAGPLVAASPVNVVVGSFLPSEQHEQKLKTQNNEVISTPAAPMSTAGPNT*