AlfalfaGEDB | Msa1196450

M. sativa cultivar zhongmu-4 / Msa1196450

KEGG: K09338
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009965 GO:0010016 GO:0010033 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0034285 GO:0042221 GO:0043565 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048598 GO:0048608 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080022 GO:0080090 GO:0090698 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1901700 GO:1903506 GO:1905392 GO:2000112 GO:2001141
PFAM: PF00046.32

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1196450	XP_013445749.1	100.000	282	0	0	1	282	1	282	0.0	586

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1196450	sp\|Q8LC03\|ATB13_ARATH	64.189	296	84	8	1	282	7	294	8.44e-119	345

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1196450	A0A072U1W4	100.000	282	0	0	1	282	1	282	0.0	586

TFs/TRs:

Gene ID	Type	Classification
Msa1196450	TF	HB-HD-ZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0005890	Msa1196450	0.814209	1.731323e-51	-8.615850e-47
Msa0007480	Msa1196450	0.800910	1.154555e-48	-8.615850e-47
Msa0018160	Msa1196450	0.805177	1.514020e-49	-8.615850e-47
Msa0036870	Msa1196450	0.823006	1.746867e-53	-8.615850e-47
Msa0043230	Msa1196450	0.817651	2.952908e-52	-8.615850e-47
Msa0074350	Msa1196450	0.845516	3.869125e-59	-8.615850e-47
Msa0074510	Msa1196450	0.835740	1.407000e-56	-8.615850e-47
Msa0075220	Msa1196450	0.801707	7.929393e-49	-8.615850e-47
Msa0087940	Msa1196450	0.803306	3.711750e-49	-8.615850e-47
Msa0090400	Msa1196450	0.834124	3.592486e-56	-8.615850e-47
Msa0117480	Msa1196450	0.834318	3.213092e-56	-8.615850e-47
Msa0625630	Msa1196450	0.804722	1.885069e-49	-8.615850e-47
Msa0626450	Msa1196450	0.827571	1.453681e-54	-8.615850e-47
Msa0626490	Msa1196450	0.824824	6.547356e-54	-8.615850e-47
Msa0637560	Msa1196450	0.810337	1.212257e-50	-8.615850e-47
Msa0637580	Msa1196450	0.807585	4.705125e-50	-8.615850e-47
Msa0652770	Msa1196450	0.817579	3.065043e-52	-8.615850e-47
Msa0663300	Msa1196450	0.806558	7.760952e-50	-8.615850e-47
Msa0664060	Msa1196450	0.807721	4.401158e-50	-8.615850e-47
Msa0664600	Msa1196450	0.827162	1.822198e-54	-8.615850e-47
Msa0683570	Msa1196450	0.816091	6.613220e-52	-8.615850e-47
Msa0700460	Msa1196450	0.845015	5.284058e-59	-8.615850e-47
Msa0724910	Msa1196450	0.804021	2.637687e-49	-8.615850e-47
Msa0727640	Msa1196450	0.807621	4.621989e-50	-8.615850e-47
Msa0738040	Msa1196450	0.824093	9.726420e-54	-8.615850e-47
Msa0756490	Msa1196450	0.803909	2.783765e-49	-8.615850e-47
Msa0768990	Msa1196450	0.815301	9.916291e-52	-8.615850e-47
Msa0795870	Msa1196450	0.813137	2.981103e-51	-8.615850e-47
Msa0829300	Msa1196450	0.814958	1.181834e-51	-8.615850e-47
Msa0829330	Msa1196450	0.830551	2.755616e-55	-8.615850e-47
Msa0833650	Msa1196450	0.808256	3.385998e-50	-8.615850e-47
Msa0835490	Msa1196450	0.823438	1.385276e-53	-8.615850e-47
Msa0134610	Msa1196450	0.811923	5.491150e-51	-8.615850e-47
Msa0136650	Msa1196450	0.828077	1.098337e-54	-8.615850e-47
Msa0137130	Msa1196450	0.803211	3.883901e-49	-8.615850e-47
Msa0137880	Msa1196450	0.840097	1.067409e-57	-8.615850e-47
Msa0146040	Msa1196450	0.815813	7.626076e-52	-8.615850e-47
Msa0182320	Msa1196450	0.827751	1.315866e-54	-8.615850e-47
Msa0230800	Msa1196450	0.804698	1.906700e-49	-8.615850e-47
Msa0378720	Msa1196450	0.805220	1.483198e-49	-8.615850e-47
Msa0380620	Msa1196450	0.818100	2.337261e-52	-8.615850e-47
Msa0428150	Msa1196450	0.832403	9.646105e-56	-8.615850e-47
Msa0430260	Msa1196450	0.827722	1.337126e-54	-8.615850e-47
Msa0436910	Msa1196450	0.808211	3.461463e-50	-8.615850e-47
Msa0462500	Msa1196450	0.838319	3.086937e-57	-8.615850e-47
Msa0474730	Msa1196450	0.809459	1.872842e-50	-8.615850e-47
Msa0475790	Msa1196450	0.830208	3.342077e-55	-8.615850e-47
Msa1003740	Msa1196450	0.805253	1.459469e-49	-8.615850e-47
Msa1003800	Msa1196450	0.828654	7.971827e-55	-8.615850e-47
Msa1003930	Msa1196450	0.807760	4.319346e-50	-8.615850e-47
Msa1018690	Msa1196450	0.802986	4.324927e-49	-8.615850e-47
Msa1021590	Msa1196450	0.801730	7.845020e-49	-8.615850e-47
Msa1034600	Msa1196450	0.837659	4.563095e-57	-8.615850e-47
Msa1034780	Msa1196450	0.829948	3.868757e-55	-8.615850e-47
Msa1037640	Msa1196450	0.804438	2.159763e-49	-8.615850e-47
Msa1041290	Msa1196450	-0.813586	2.374526e-51	-8.615850e-47
Msa1042160	Msa1196450	0.817423	3.323746e-52	-8.615850e-47
Msa1042190	Msa1196450	0.817763	2.785714e-52	-8.615850e-47
Msa1044680	Msa1196450	0.823594	1.273772e-53	-8.615850e-47
Msa1045140	Msa1196450	0.831297	1.808310e-55	-8.615850e-47
Msa1074370	Msa1196450	0.869689	2.565676e-66	-8.615850e-47
Msa1080000	Msa1196450	0.822395	2.423851e-53	-8.615850e-47
Msa1096960	Msa1196450	0.847110	1.422497e-59	-8.615850e-47
Msa1105640	Msa1196450	0.810161	1.322427e-50	-8.615850e-47
Msa0239190	Msa1196450	0.804241	2.374012e-49	-8.615850e-47
Msa0255090	Msa1196450	0.844251	8.484932e-59	-8.615850e-47
Msa0259380	Msa1196450	0.842694	2.211492e-58	-8.615850e-47
Msa0267110	Msa1196450	0.852191	5.426751e-61	-8.615850e-47
Msa0292400	Msa1196450	0.827259	1.726674e-54	-8.615850e-47
Msa0294160	Msa1196450	0.815916	7.233351e-52	-8.615850e-47
Msa0304930	Msa1196450	0.801496	8.760523e-49	-8.615850e-47
Msa0349710	Msa1196450	0.817545	3.119619e-52	-8.615850e-47
Msa1113340	Msa1196450	0.844231	8.593512e-59	-8.615850e-47
Msa1114900	Msa1196450	0.823646	1.238167e-53	-8.615850e-47
Msa1115670	Msa1196450	0.807573	4.732505e-50	-8.615850e-47
Msa1132840	Msa1196450	0.814602	1.417351e-51	-8.615850e-47
Msa1140220	Msa1196450	0.830056	3.640478e-55	-8.615850e-47
Msa1162150	Msa1196450	0.817187	3.755159e-52	-8.615850e-47
Msa1167470	Msa1196450	0.822345	2.489547e-53	-8.615850e-47
Msa1174840	Msa1196450	0.818560	1.839070e-52	-8.615850e-47
Msa1192090	Msa1196450	0.865266	6.688884e-65	-8.615850e-47
Msa1196440	Msa1196450	0.866950	1.958895e-65	-8.615850e-47
Msa1196450	Msa1204340	0.803699	3.077800e-49	-8.615850e-47
Msa1196450	Msa1216090	0.805428	1.341411e-49	-8.615850e-47
Msa1196450	Msa1216110	0.850237	1.933089e-60	-8.615850e-47
Msa1196450	Msa1234710	0.825898	3.645924e-54	-8.615850e-47
Msa1196450	Msa1240770	0.815887	7.341208e-52	-8.615850e-47
Msa1196450	Msa1287200	0.827164	1.819499e-54	-8.615850e-47
Msa1196450	Msa1303560	0.803824	2.899085e-49	-8.615850e-47
Msa1196450	Msa1303650	0.809498	1.836576e-50	-8.615850e-47
Msa1196450	Msa1315130	0.835619	1.509760e-56	-8.615850e-47
Msa1196450	Msa1315160	0.829828	4.137396e-55	-8.615850e-47
Msa1196450	Msa1321340	0.803913	2.778252e-49	-8.615850e-47
Msa1196450	Msa1333480	0.802601	5.193564e-49	-8.615850e-47
Msa1196450	Msa1363260	0.801903	7.229476e-49	-8.615850e-47
Msa1196450	Msa1369150	0.804759	1.851182e-49	-8.615850e-47
Msa1196450	Msa1393780	0.800598	1.337094e-48	-8.615850e-47
Msa1196450	Msa1412000	0.811256	7.667472e-51	-8.615850e-47
Msa1196450	Msa1440960	0.823333	1.465965e-53	-8.615850e-47
Msa1196450	Msa1454140	0.839074	1.968964e-57	-8.615850e-47
Msa0483910	Msa1196450	0.825570	4.363051e-54	-8.615850e-47
Msa0500380	Msa1196450	0.821497	3.912026e-53	-8.615850e-47
Msa0527860	Msa1196450	0.827734	1.328487e-54	-8.615850e-47
Msa0530780	Msa1196450	0.809497	1.838088e-50	-8.615850e-47
Msa0532070	Msa1196450	0.800733	1.255082e-48	-8.615850e-47
Msa0563940	Msa1196450	0.813593	2.366873e-51	-8.615850e-47
Msa0580370	Msa1196450	0.813438	2.559069e-51	-8.615850e-47
Msa0582450	Msa1196450	0.805680	1.187572e-49	-8.615850e-47
Msa0586190	Msa1196450	0.806796	6.913300e-50	-8.615850e-47
Msa0587620	Msa1196450	0.804264	2.347715e-49	-8.615850e-47
Msa0597790	Msa1196450	0.812852	3.441737e-51	-8.615850e-47
Msa0600930	Msa1196450	0.839525	1.504369e-57	-8.615850e-47
Msa0869780	Msa1196450	0.808217	3.452627e-50	-8.615850e-47
Msa0876310	Msa1196450	0.817683	2.904614e-52	-8.615850e-47
Msa0894790	Msa1196450	0.803128	4.040800e-49	-8.615850e-47
Msa0942640	Msa1196450	0.806529	7.870693e-50	-8.615850e-47
Msa0945110	Msa1196450	0.830979	2.164076e-55	-8.615850e-47
Msa0945190	Msa1196450	0.823670	1.222297e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1196450	MtrunA17_Chr8g0362151	100.000	282	0	0	1	282	1	282	0.0	586
Msa1196450	MtrunA17_Chr5g0416391	81.818	286	42	6	1	282	4	283	1.33e-150	422
Msa1196450	MtrunA17_Chr6g0453021	50.498	301	98	10	1	279	1	272	1.69e-84	254
Msa1196450	MtrunA17_Chr7g0217111	52.465	284	113	9	1	275	1	271	3.95e-82	248
Msa1196450	MtrunA17_Chr3g0123691	51.656	151	60	4	33	180	26	166	3.66e-39	139
Msa1196450	MtrunA17_Chr6g0450991	64.948	97	34	0	76	172	66	162	6.53e-39	137
Msa1196450	MtrunA17_Chr3g0119291	49.689	161	62	3	64	213	46	198	1.03e-37	134
Msa1196450	MtrunA17_Chr5g0415941	66.667	96	32	0	77	172	87	182	4.74e-37	134
Msa1196450	MtrunA17_Chr4g0060031	58.475	118	46	2	52	169	73	187	9.47e-37	133
Msa1196450	MtrunA17_Chr8g0381741	62.245	98	37	0	75	172	52	149	2.19e-36	132
Msa1196450	MtrunA17_Chr8g0362751	65.625	96	33	0	77	172	89	184	3.70e-36	130
Msa1196450	MtrunA17_Chr7g0265521	45.652	138	59	2	44	172	21	151	5.79e-33	120
Msa1196450	MtrunA17_Chr1g0179581	56.522	92	38	1	65	156	18	107	1.03e-27	103
Msa1196450	MtrunA17_Chr3g0127511	53.846	91	42	0	79	169	40	130	3.88e-27	105
Msa1196450	MtrunA17_Chr8g0346251	38.000	150	82	3	84	222	48	197	4.85e-25	100
Msa1196450	MtrunA17_Chr4g0055851	40.496	121	69	1	43	163	89	206	5.94e-21	90.1
Msa1196450	MtrunA17_Chr5g0403691	46.809	94	50	0	77	170	53	146	2.30e-19	84.3
Msa1196450	MtrunA17_Chr5g0403701	47.778	90	47	0	77	166	53	142	2.34e-19	84.0
Msa1196450	MtrunA17_Chr5g0399491	41.463	123	70	2	43	163	100	222	7.10e-19	84.3
Msa1196450	MtrunA17_Chr4g0054421	45.263	95	52	0	69	163	119	213	8.27e-19	84.0
Msa1196450	MtrunA17_Chr7g0258831	46.939	98	48	1	77	174	160	253	3.29e-18	82.8
Msa1196450	MtrunA17_Chr5g0400781	44.000	100	56	0	69	168	115	214	3.74e-18	82.0
Msa1196450	MtrunA17_Chr1g0175651	42.609	115	62	1	60	174	137	247	4.34e-18	82.4
Msa1196450	MtrunA17_Chr2g0308611	39.669	121	69	1	54	174	177	293	1.64e-17	81.3
Msa1196450	MtrunA17_Chr8g0335131	46.739	92	49	0	77	168	146	237	2.28e-17	80.5
Msa1196450	MtrunA17_Chr4g0071411	45.882	85	46	0	80	164	91	175	6.05e-17	77.8
Msa1196450	MtrunA17_Chr1g0151971	45.455	88	48	0	77	164	130	217	1.11e-16	78.2
Msa1196450	MtrunA17_Chr4g0044091	43.434	99	52	1	77	175	65	159	2.40e-15	72.8
Msa1196450	MtrunA17_Chr3g0135331	48.611	72	37	0	77	148	145	216	4.26e-14	71.2
Msa1196450	MtrunA17_Chr2g0299031	37.615	109	64	2	59	163	60	168	9.34e-12	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1196450	AT1G69780.1	64.189	296	84	8	1	282	7	294	8.58e-120	345
Msa1196450	AT1G26960.1	55.363	289	81	9	1	282	8	255	5.23e-95	281
Msa1196450	AT3G01220.1	48.057	283	114	9	1	275	16	273	1.07e-74	230
Msa1196450	AT5G15150.1	44.300	307	111	11	1	275	22	300	1.47e-71	223
Msa1196450	AT4G40060.1	57.576	132	52	2	59	186	39	170	4.71e-40	141
Msa1196450	AT5G65310.2	48.824	170	70	4	52	206	19	186	7.52e-40	140
Msa1196450	AT5G65310.1	48.824	170	70	4	52	206	37	204	8.71e-40	141
Msa1196450	AT3G01470.1	48.052	154	65	1	34	172	10	163	6.06e-39	137
Msa1196450	AT2G22430.1	66.667	99	33	0	74	172	59	157	8.64e-39	138
Msa1196450	AT1G27045.1	40.306	196	94	5	8	195	12	192	2.63e-31	116
Msa1196450	AT1G27045.4	42.857	161	75	4	8	166	12	157	1.64e-29	112
Msa1196450	AT1G27045.2	48.800	125	58	1	77	195	17	141	4.02e-28	107
Msa1196450	AT1G27045.5	48.800	125	58	1	77	195	25	149	4.60e-28	107
Msa1196450	AT1G27045.3	48.800	125	58	1	77	195	25	149	4.60e-28	107
Msa1196450	AT2G46680.1	52.688	93	44	0	78	170	33	125	8.27e-28	108
Msa1196450	AT2G46680.2	53.333	90	42	0	78	167	33	122	4.57e-27	106
Msa1196450	AT3G61890.1	51.111	90	44	0	80	169	33	122	1.28e-25	102
Msa1196450	AT5G03790.1	52.273	88	42	0	77	164	77	164	1.58e-24	99.0
Msa1196450	AT5G03790.2	55.000	80	36	0	77	156	77	156	1.49e-23	95.5
Msa1196450	AT2G18550.1	46.341	123	56	3	77	196	61	176	1.65e-22	93.2
Msa1196450	AT2G36610.1	43.590	117	51	3	58	166	59	168	1.95e-22	92.0
Msa1196450	AT4G36740.1	48.544	103	46	1	68	170	46	141	6.98e-22	91.3
Msa1196450	AT4G36740.2	48.544	103	46	1	68	170	47	142	7.50e-22	91.3
Msa1196450	AT5G66700.1	45.378	119	56	2	61	172	57	173	3.41e-21	89.7
Msa1196450	AT5G53980.1	44.554	101	56	0	72	172	6	106	1.04e-20	86.7
Msa1196450	AT2G01430.1	43.396	106	60	0	58	163	120	225	5.02e-18	82.0
Msa1196450	AT5G47370.1	42.708	96	55	0	69	164	122	217	2.26e-17	80.5
Msa1196450	AT4G37790.1	41.228	114	63	1	61	174	110	219	3.87e-17	79.7
Msa1196450	AT2G22800.1	46.667	90	48	0	75	164	111	200	4.95e-17	79.3
Msa1196450	AT5G06710.1	43.434	99	56	0	66	164	179	277	8.23e-17	79.7
Msa1196450	AT4G17460.1	41.414	99	58	0	65	163	123	221	1.18e-16	78.6
Msa1196450	AT3G60390.1	42.991	107	57	1	64	170	149	251	1.62e-16	78.6
Msa1196450	AT2G44910.1	44.681	94	48	1	77	170	163	252	5.28e-15	74.3
Msa1196450	AT4G16780.1	42.157	102	55	1	69	170	121	218	8.91e-15	73.2
Msa1196450	AT1G70920.1	37.903	124	69	2	57	180	50	165	9.54e-15	71.6
Msa1196450	AT1G70920.2	37.903	124	69	2	57	180	20	135	1.71e-14	70.5
Msa1196450	AT2G01430.2	50.704	71	35	0	59	129	121	191	2.56e-13	67.8

Find 64 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCCTTCTGTTTCTTTATTA+AGG	0.139873	8_1:-45654920	None:intergenic
GATTGCATGCAATTATTTAA+TGG	0.196144	8_1:+45655691	Msa1196450:three_prime_UTR
TTTCAAAAGGAAATGGAAAT+TGG	0.200276	8_1:-45653340	None:intergenic
TCAATTAATTGATCAAATAA+AGG	0.264717	8_1:+45655225	Msa1196450:exon
TTGATCTCTTGGTGCAAGTT+TGG	0.272211	8_1:-45653485	None:intergenic
CTGAATGTCTTGTCTTGAAT+TGG	0.288821	8_1:-45655099	None:intergenic
TTGCAACTTTACATATAATA+AGG	0.291015	8_1:+45653213	Msa1196450:five_prime_UTR
TGAAATCAAAAGTGCATGTT+TGG	0.326942	8_1:+45655289	Msa1196450:three_prime_UTR
ATGAATATTTCAAAAGGAAA+TGG	0.330552	8_1:-45653347	None:intergenic
GGATTTGAGTAAAGAACTAT+TGG	0.342549	8_1:+45653531	Msa1196450:five_prime_UTR
AAGACAATATGATACTATTA+AGG	0.363112	8_1:+45654188	Msa1196450:CDS
GTTTGTAGCATGAAATTTGC+TGG	0.365160	8_1:-45653585	None:intergenic
AACCAAGACAGATTGCTATA+TGG	0.383913	8_1:+45654103	Msa1196450:CDS
TCCGATGATGGATCGCAGTT+AGG	0.391738	8_1:+45653964	Msa1196450:CDS
GTCTTGAAGAAGATGGAAAT+AGG	0.399749	8_1:-45655046	None:intergenic
CAATTAATTGATCAAATAAA+GGG	0.400147	8_1:+45655226	Msa1196450:exon
AATCTCTTCCTTTATTTCTG+TGG	0.403872	8_1:-45655715	None:intergenic
AGCATTTGACAGTGAATGTT+CGG	0.411555	8_1:-45655434	None:intergenic
ACAAGAGAAGATTGATCTCT+TGG	0.413112	8_1:-45653496	None:intergenic
CCGATGATGGATCGCAGTTA+GGG	0.414857	8_1:+45653965	Msa1196450:CDS
ATGCAGCTAGCAAGGGCTCT+TGG	0.429987	8_1:+45654075	Msa1196450:CDS
TAAACTTTGATGTATAGAAT+AGG	0.433223	8_1:+45655480	Msa1196450:three_prime_UTR
ATCTCTTCCTTTATTTCTGT+GGG	0.438371	8_1:-45655714	None:intergenic
TGGATGATCAATCTGGACTA+TGG	0.444127	8_1:+45655180	Msa1196450:CDS
CCATCTTCTTCAAGACCAGC+AGG	0.460199	8_1:+45655053	Msa1196450:CDS
CTTGTCTGTTGAGTAGATAA+AGG	0.476490	8_1:-45655017	None:intergenic
GTTTCGATAGTAATAGATCA+TGG	0.478644	8_1:+45655625	Msa1196450:three_prime_UTR
TTCTCTTGTTATTCAACATG+TGG	0.480787	8_1:+45653510	Msa1196450:five_prime_UTR
AGCTGATGAGGAACTCCTGC+TGG	0.491266	8_1:-45655068	None:intergenic
AAATATTTGATAGTACTAGT+TGG	0.492736	8_1:+45655663	Msa1196450:three_prime_UTR
TGTGCCATGGATGATCAATC+TGG	0.494499	8_1:+45655173	Msa1196450:CDS
CCCTAACTGCGATCCATCAT+CGG	0.502143	8_1:-45653965	None:intergenic
CTATATGGTTTCAAAACAGA+AGG	0.506981	8_1:+45654118	Msa1196450:CDS
ATAATTGAGTTGAGTGAAGG+TGG	0.507171	8_1:-45653636	None:intergenic
CCTGCTGGTCTTGAAGAAGA+TGG	0.521993	8_1:-45655053	None:intergenic
ATCTGGACTATGGCCATGGT+TGG	0.522424	8_1:+45655190	Msa1196450:CDS
AACCATATAGCAATCTGTCT+TGG	0.526039	8_1:-45654105	None:intergenic
AGAGGAAAATGCAGCTAGCA+AGG	0.526858	8_1:+45654067	Msa1196450:CDS
CATCAAAGTTTACCAAAACA+AGG	0.536310	8_1:-45655469	None:intergenic
TTAAGGTTGATTGATTCAGT+TGG	0.542418	8_1:-45654903	None:intergenic
GAGGAAGAATTATCCGATGA+TGG	0.563562	8_1:+45653952	Msa1196450:CDS
GGTTTCAAAACAGAAGGGCT+AGG	0.568856	8_1:+45654124	Msa1196450:CDS
TAGTCCAGATTGATCATCCA+TGG	0.576768	8_1:-45655177	None:intergenic
CTCTTGGTGCAAGTTTGGAA+AGG	0.581165	8_1:-45653480	None:intergenic
AATTGGAAGAAAACGTTGAG+AGG	0.589855	8_1:-45653323	None:intergenic
TTGGTTTGAAAGAGCTGATG+AGG	0.592238	8_1:-45655080	None:intergenic
AACCTTAATAAAGAAACAGA+AGG	0.601474	8_1:+45654918	Msa1196450:CDS
GTTATAATTGAGTTGAGTGA+AGG	0.601931	8_1:-45653639	None:intergenic
GAAGAAGAGAAGGCTTAACA+TGG	0.612268	8_1:+45653990	Msa1196450:CDS
ATCAATCTGGACTATGGCCA+TGG	0.615676	8_1:+45655186	Msa1196450:CDS
AGTTAGGGGAGAAGAAGAGA+AGG	0.616244	8_1:+45653980	Msa1196450:CDS
ATTGAGTTGAGTGAAGGTGG+AGG	0.620969	8_1:-45653633	None:intergenic
TAGGTGGAAGACAAAACAGT+TGG	0.622940	8_1:+45654143	Msa1196450:CDS
TATATGGTTTCAAAACAGAA+GGG	0.626685	8_1:+45654119	Msa1196450:CDS
GAGGAAAATGCAGCTAGCAA+GGG	0.631443	8_1:+45654068	Msa1196450:CDS
AAGTGCTGTTGCTCCAACCA+TGG	0.633621	8_1:-45655203	None:intergenic
GTAATCTTGAGGTGCACATG+AGG	0.639812	8_1:-45653661	None:intergenic
CGATGATGGATCGCAGTTAG+GGG	0.649440	8_1:+45653966	Msa1196450:CDS
GAAACAAGCTTGAACCTGAG+AGG	0.652955	8_1:+45654049	Msa1196450:CDS
AAAGTACCATGGTAATCTTG+AGG	0.656226	8_1:-45653672	None:intergenic
TGGAGAAGAAGCTAATGTCG+AGG	0.659865	8_1:+45653933	Msa1196450:CDS
TTCAAAACAGAAGGGCTAGG+TGG	0.663747	8_1:+45654127	Msa1196450:CDS
TGTGCACCTCAAGATTACCA+TGG	0.714676	8_1:+45653666	Msa1196450:CDS
TCAAGCTTGATATTTCAACA+AGG	0.737294	8_1:+45654976	Msa1196450:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTATATGAATATTTCAAA+AGG	-	chr8_1:45653356-45653375	None:intergenic	10.0%
!!!	TAATTTTTTTTACCAAAATG+AGG	-	chr8_1:45654760-45654779	None:intergenic	15.0%
!!	TCAATTAATTGATCAAATAA+AGG	+	chr8_1:45655225-45655244	Msa1196450:exon	15.0%
!!	CAATTAATTGATCAAATAAA+GGG	+	chr8_1:45655226-45655245	Msa1196450:exon	15.0%
!!!	TATATCAAGCATAATTTTTA+TGG	+	chr8_1:45655506-45655525	Msa1196450:three_prime_UTR	15.0%
!!	TTGCAACTTTACATATAATA+AGG	+	chr8_1:45653213-45653232	Msa1196450:five_prime_UTR	20.0%
!!	ATGAATATTTCAAAAGGAAA+TGG	-	chr8_1:45653350-45653369	None:intergenic	20.0%
!!	ACATTGCTAGATTTAAATAA+AGG	-	chr8_1:45653804-45653823	None:intergenic	20.0%
!!	AAGACAATATGATACTATTA+AGG	+	chr8_1:45654188-45654207	Msa1196450:CDS	20.0%
!!!	AACTTTTTATTCTTTACTCA+TGG	+	chr8_1:45654269-45654288	Msa1196450:intron	20.0%
!!	GACACATAAATTAACAAATA+TGG	-	chr8_1:45654428-45654447	None:intergenic	20.0%
!!	AAATTGATGAAAGGAAATAA+AGG	-	chr8_1:45654642-45654661	None:intergenic	20.0%
!!	TTGTTAAAGTTATCATTAGT+TGG	-	chr8_1:45654667-45654686	None:intergenic	20.0%
!!	TAAACTTTGATGTATAGAAT+AGG	+	chr8_1:45655480-45655499	Msa1196450:three_prime_UTR	20.0%
!!!	AAATATTTGATAGTACTAGT+TGG	+	chr8_1:45655663-45655682	Msa1196450:three_prime_UTR	20.0%
!	TTTCAAAAGGAAATGGAAAT+TGG	-	chr8_1:45653343-45653362	None:intergenic	25.0%
!!!	ATTTTTTGCTTGCAATGATA+TGG	+	chr8_1:45653554-45653573	Msa1196450:exon	25.0%
!!	CATAGATCTTTTTCCTAAAA+AGG	-	chr8_1:45653893-45653912	None:intergenic	25.0%
!!!	AAAAGATCTATGTCATTTTC+AGG	+	chr8_1:45653901-45653920	Msa1196450:CDS	25.0%
!!!	AAAGATCTATGTCATTTTCA+GGG	+	chr8_1:45653902-45653921	Msa1196450:CDS	25.0%
!	TATATGGTTTCAAAACAGAA+GGG	+	chr8_1:45654119-45654138	Msa1196450:CDS	25.0%
!!!	CTTTTAGAACAACATTAGAT+TGG	-	chr8_1:45654357-45654376	None:intergenic	25.0%
!!	AAGAGATGTTCAAGTATTTT+GGG	+	chr8_1:45654484-45654503	Msa1196450:intron	25.0%
!!!	GTAAAAAGCTTTTTTTTGCT+TGG	-	chr8_1:45654819-45654838	None:intergenic	25.0%
!	AACCTTAATAAAGAAACAGA+AGG	+	chr8_1:45654918-45654937	Msa1196450:CDS	25.0%
!!	ATCCTTCTGTTTCTTTATTA+AGG	-	chr8_1:45654923-45654942	None:intergenic	25.0%
!	AAAAATTGATCACATGGTTA+AGG	+	chr8_1:45655127-45655146	Msa1196450:CDS	25.0%
!!	AGAAGAAATAGTTTTCAGAT+TGG	+	chr8_1:45655250-45655269	Msa1196450:three_prime_UTR	25.0%
!!!	AAAGTTGAAAAACCTTGTTT+TGG	+	chr8_1:45655457-45655476	Msa1196450:three_prime_UTR	25.0%
!!	AAGCATAATTTTTATGGTTG+AGG	+	chr8_1:45655512-45655531	Msa1196450:three_prime_UTR	25.0%
!	GATTGCATGCAATTATTTAA+TGG	+	chr8_1:45655691-45655710	Msa1196450:three_prime_UTR	25.0%
!	TAGCATAAGATGTTTTGCAT+TGG	+	chr8_1:45653409-45653428	Msa1196450:five_prime_UTR	30.0%
!!	TTCTCTTGTTATTCAACATG+TGG	+	chr8_1:45653510-45653529	Msa1196450:five_prime_UTR	30.0%
	GGATTTGAGTAAAGAACTAT+TGG	+	chr8_1:45653531-45653550	Msa1196450:five_prime_UTR	30.0%
!!	GTTATAATTGAGTTGAGTGA+AGG	-	chr8_1:45653642-45653661	None:intergenic	30.0%
!!	CATGTTTTAAATGTTGATGC+AGG	+	chr8_1:45653856-45653875	Msa1196450:intron	30.0%
	CTATATGGTTTCAAAACAGA+AGG	+	chr8_1:45654118-45654137	Msa1196450:CDS	30.0%
!	CAAGAGATGTTCAAGTATTT+TGG	+	chr8_1:45654483-45654502	Msa1196450:intron	30.0%
!!	TAGTTGGTGAAATTGATGAA+AGG	-	chr8_1:45654651-45654670	None:intergenic	30.0%
	GAAAGTAACTGTGTAATGAT+AGG	-	chr8_1:45654705-45654724	None:intergenic	30.0%
!	TTAAGGTTGATTGATTCAGT+TGG	-	chr8_1:45654906-45654925	None:intergenic	30.0%
	TCAAGCTTGATATTTCAACA+AGG	+	chr8_1:45654976-45654995	Msa1196450:CDS	30.0%
	GTGTCAAAAAATTGATCACA+TGG	+	chr8_1:45655121-45655140	Msa1196450:CDS	30.0%
!	TGAAATCAAAAGTGCATGTT+TGG	+	chr8_1:45655289-45655308	Msa1196450:three_prime_UTR	30.0%
	CATCAAAGTTTACCAAAACA+AGG	-	chr8_1:45655472-45655491	None:intergenic	30.0%
	GTTTCGATAGTAATAGATCA+TGG	+	chr8_1:45655625-45655644	Msa1196450:three_prime_UTR	30.0%
	AATTGGAAGAAAACGTTGAG+AGG	-	chr8_1:45653326-45653345	None:intergenic	35.0%
	ACAAGAGAAGATTGATCTCT+TGG	-	chr8_1:45653499-45653518	None:intergenic	35.0%
	GTTTGTAGCATGAAATTTGC+TGG	-	chr8_1:45653588-45653607	None:intergenic	35.0%
!!	ATAATTGAGTTGAGTGAAGG+TGG	-	chr8_1:45653639-45653658	None:intergenic	35.0%
!	AAAGTACCATGGTAATCTTG+AGG	-	chr8_1:45653675-45653694	None:intergenic	35.0%
!!	TCTAGAGAAAGAAAGTACCA+TGG	-	chr8_1:45653686-45653705	None:intergenic	35.0%
!!	GTTTTAAATGTTGATGCAGG+AGG	+	chr8_1:45653859-45653878	Msa1196450:intron	35.0%
!!	TCATTTTCAGGGATAGAACT+TGG	+	chr8_1:45653913-45653932	Msa1196450:CDS	35.0%
!!!	CTTGAGAAGAGTTTTGAGTT+AGG	+	chr8_1:45654027-45654046	Msa1196450:CDS	35.0%
	AACCAAGACAGATTGCTATA+TGG	+	chr8_1:45654103-45654122	Msa1196450:CDS	35.0%
	AACCATATAGCAATCTGTCT+TGG	-	chr8_1:45654108-45654127	None:intergenic	35.0%
	GAATCAAAAACTTCAGACTG+AGG	+	chr8_1:45654236-45654255	Msa1196450:CDS	35.0%
	AAAGAAAACATGTCCTTGAG+TGG	-	chr8_1:45654727-45654746	None:intergenic	35.0%
!	AGGACATGTTTTCTTTTGCA+TGG	+	chr8_1:45654731-45654750	Msa1196450:intron	35.0%
!	TTTGCATGGTTTCCTCATTT+TGG	+	chr8_1:45654745-45654764	Msa1196450:intron	35.0%
!	TCAATGCTTGTTGCAGATAT+TGG	+	chr8_1:45654863-45654882	Msa1196450:intron	35.0%
	CTTGTCTGTTGAGTAGATAA+AGG	-	chr8_1:45655020-45655039	None:intergenic	35.0%
	GTCTTGAAGAAGATGGAAAT+AGG	-	chr8_1:45655049-45655068	None:intergenic	35.0%
	CTGAATGTCTTGTCTTGAAT+TGG	-	chr8_1:45655102-45655121	None:intergenic	35.0%
	AGCATTTGACAGTGAATGTT+CGG	-	chr8_1:45655437-45655456	None:intergenic	35.0%
!	TTGATCTCTTGGTGCAAGTT+TGG	-	chr8_1:45653488-45653507	None:intergenic	40.0%
	TTAAATGTTGATGCAGGAGG+AGG	+	chr8_1:45653862-45653881	Msa1196450:intron	40.0%
	GAGGAAGAATTATCCGATGA+TGG	+	chr8_1:45653952-45653971	Msa1196450:CDS	40.0%
	GAAGAAGAGAAGGCTTAACA+TGG	+	chr8_1:45653990-45654009	Msa1196450:CDS	40.0%
	TAGGTGGAAGACAAAACAGT+TGG	+	chr8_1:45654143-45654162	Msa1196450:CDS	40.0%
!	ACACAGTTACTTTCCACTCA+AGG	+	chr8_1:45654711-45654730	Msa1196450:intron	40.0%
!	TTGGTTTGAAAGAGCTGATG+AGG	-	chr8_1:45655083-45655102	None:intergenic	40.0%
!	GAGTAACATGTTTTGTGCCA+TGG	+	chr8_1:45655160-45655179	Msa1196450:CDS	40.0%
	TAGTCCAGATTGATCATCCA+TGG	-	chr8_1:45655180-45655199	None:intergenic	40.0%
	TGGATGATCAATCTGGACTA+TGG	+	chr8_1:45655180-45655199	Msa1196450:CDS	40.0%
!	CTCTTGGTGCAAGTTTGGAA+AGG	-	chr8_1:45653483-45653502	None:intergenic	45.0%
!!	ATTGAGTTGAGTGAAGGTGG+AGG	-	chr8_1:45653636-45653655	None:intergenic	45.0%
	GTAATCTTGAGGTGCACATG+AGG	-	chr8_1:45653664-45653683	None:intergenic	45.0%
	TGTGCACCTCAAGATTACCA+TGG	+	chr8_1:45653666-45653685	Msa1196450:CDS	45.0%
	AACTGCAATTGAGGCTGCAT+CGG	-	chr8_1:45653750-45653769	None:intergenic	45.0%
	CGACAACACAACTGCAATTG+AGG	-	chr8_1:45653759-45653778	None:intergenic	45.0%
	CAATTGCAGTTGTGTTGTCG+TGG	+	chr8_1:45653759-45653778	Msa1196450:intron	45.0%
!!!	GGAGGAGGAGTATCCTTTTT+AGG	+	chr8_1:45653877-45653896	Msa1196450:intron	45.0%
	TGGAGAAGAAGCTAATGTCG+AGG	+	chr8_1:45653933-45653952	Msa1196450:CDS	45.0%
	AGTTAGGGGAGAAGAAGAGA+AGG	+	chr8_1:45653980-45653999	Msa1196450:CDS	45.0%
	GAAACAAGCTTGAACCTGAG+AGG	+	chr8_1:45654049-45654068	Msa1196450:CDS	45.0%
	AGAGGAAAATGCAGCTAGCA+AGG	+	chr8_1:45654067-45654086	Msa1196450:CDS	45.0%
	GAGGAAAATGCAGCTAGCAA+GGG	+	chr8_1:45654068-45654087	Msa1196450:CDS	45.0%
	GGTTTCAAAACAGAAGGGCT+AGG	+	chr8_1:45654124-45654143	Msa1196450:CDS	45.0%
	TTCAAAACAGAAGGGCTAGG+TGG	+	chr8_1:45654127-45654146	Msa1196450:CDS	45.0%
	CGACAAGAGCTGAATTTCAG+TGG	+	chr8_1:45654320-45654339	Msa1196450:intron	45.0%
	TGTGCCATGGATGATCAATC+TGG	+	chr8_1:45655173-45655192	Msa1196450:CDS	45.0%
	ATCAATCTGGACTATGGCCA+TGG	+	chr8_1:45655186-45655205	Msa1196450:CDS	45.0%
!!!	AATGTAATATTATTTTTTTA+TGG	+	chr8_1:45653819-45653838	Msa1196450:intron	5.0%
!!!	ATGTAATATTATTTTTTTAT+GGG	+	chr8_1:45653820-45653839	Msa1196450:intron	5.0%
!!!	TTTTTCTATAAAATAATAAA+AGG	+	chr8_1:45655542-45655561	Msa1196450:three_prime_UTR	5.0%
!!!	TTTTCTATAAAATAATAAAA+GGG	+	chr8_1:45655543-45655562	Msa1196450:three_prime_UTR	5.0%
!!!	TTTTTTTTTTTTTTTTCATT+TGG	+	chr8_1:45655573-45655592	Msa1196450:three_prime_UTR	5.0%
!!	TCCGATGATGGATCGCAGTT+AGG	+	chr8_1:45653964-45653983	Msa1196450:CDS	50.0%
	CCCTAACTGCGATCCATCAT+CGG	-	chr8_1:45653968-45653987	None:intergenic	50.0%
!!	CCGATGATGGATCGCAGTTA+GGG	+	chr8_1:45653965-45653984	Msa1196450:CDS	50.0%
!!	CGATGATGGATCGCAGTTAG+GGG	+	chr8_1:45653966-45653985	Msa1196450:CDS	50.0%
!	GCTAGCTGCATTTTCCTCTC+AGG	-	chr8_1:45654066-45654085	None:intergenic	50.0%
!!	CCTGCTGGTCTTGAAGAAGA+TGG	-	chr8_1:45655056-45655075	None:intergenic	50.0%
	CCATCTTCTTCAAGACCAGC+AGG	+	chr8_1:45655053-45655072	Msa1196450:CDS	50.0%
	ATCTGGACTATGGCCATGGT+TGG	+	chr8_1:45655190-45655209	Msa1196450:CDS	50.0%
!	AAGTGCTGTTGCTCCAACCA+TGG	-	chr8_1:45655206-45655225	None:intergenic	50.0%
	TGTTGTCGTGGCTCAGATCG+CGG	+	chr8_1:45653771-45653790	Msa1196450:intron	55.0%
	ATGCAGCTAGCAAGGGCTCT+TGG	+	chr8_1:45654075-45654094	Msa1196450:CDS	55.0%
	AGCTGATGAGGAACTCCTGC+TGG	-	chr8_1:45655071-45655090	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	45653207	45655724	45653207	ID=Msa1196450;Name=Msa1196450
chr8_1	mRNA	45653207	45655724	45653207	ID=Msa1196450-mRNA-1;Parent=Msa1196450;Name=Msa1196450-mRNA-1;_AED=0.05;_eAED=0.05;_QI=366\|1\|1\|1\|1\|1\|3\|492\|282
chr8_1	exon	45653207	45653687	45653207	ID=Msa1196450-mRNA-1:exon:14594;Parent=Msa1196450-mRNA-1
chr8_1	exon	45653878	45654257	45653878	ID=Msa1196450-mRNA-1:exon:14595;Parent=Msa1196450-mRNA-1
chr8_1	exon	45654879	45655724	45654879	ID=Msa1196450-mRNA-1:exon:14596;Parent=Msa1196450-mRNA-1
chr8_1	five_prime_UTR	45653207	45653572	45653207	ID=Msa1196450-mRNA-1:five_prime_utr;Parent=Msa1196450-mRNA-1
chr8_1	CDS	45653573	45653687	45653573	ID=Msa1196450-mRNA-1:cds;Parent=Msa1196450-mRNA-1
chr8_1	CDS	45653878	45654257	45653878	ID=Msa1196450-mRNA-1:cds;Parent=Msa1196450-mRNA-1
chr8_1	CDS	45654879	45655232	45654879	ID=Msa1196450-mRNA-1:cds;Parent=Msa1196450-mRNA-1
chr8_1	three_prime_UTR	45655233	45655724	45655233	ID=Msa1196450-mRNA-1:three_prime_utr;Parent=Msa1196450-mRNA-1

Gene Sequence

>Msa1196450

ATGGCGTTCTTTCCAGCAAATTTCATGCTACAAACTTCTCATCAAGATGAACATCATCAACCTCCACCTTCACTCAACTCAATTATAACCTCATGTGCACCTCAAGATTACCATGGAGGAGGAGTATCCTTTTTAGGAAAAAGATCTATGTCATTTTCAGGGATAGAACTTGGAGAAGAAGCTAATGTCGAGGAAGAATTATCCGATGATGGATCGCAGTTAGGGGAGAAGAAGAGAAGGCTTAACATGGAACAAGTGAAGACACTTGAGAAGAGTTTTGAGTTAGGAAACAAGCTTGAACCTGAGAGGAAAATGCAGCTAGCAAGGGCTCTTGGATTGCAACCAAGACAGATTGCTATATGGTTTCAAAACAGAAGGGCTAGGTGGAAGACAAAACAGTTGGAGAAAGACTATGATGTTCTCAAAAGACAATATGATACTATTAAGGCTGATAATGATGCACTTCAAGCTCAGAATCAAAAACTTCAGACTGAGATATTGGCACTGAAAAATAGAGAACCAACTGAATCAATCAACCTTAATAAAGAAACAGAAGGATCAAGCAGCAACAGAAGTGAAAACAGTTCAGATATCAAGCTTGATATTTCAACAAGGACACAAGCTATTGATAGTCCTTTATCTACTCAACAGACAAGCATAAACCTATTTCCATCTTCTTCAAGACCAGCAGGAGTTCCTCATCAGCTCTTTCAAACCAATTCAAGACAAGACATTCAGTGTCAAAAAATTGATCACATGGTTAAGGAAGAGAGCTTGAGTAACATGTTTTGTGCCATGGATGATCAATCTGGACTATGGCCATGGTTGGAGCAACAGCACTTCAATTAA

Protein sequence

>Msa1196450

MAFFPANFMLQTSHQDEHHQPPPSLNSIITSCAPQDYHGGGVSFLGKRSMSFSGIELGEEANVEEELSDDGSQLGEKKRRLNMEQVKTLEKSFELGNKLEPERKMQLARALGLQPRQIAIWFQNRRARWKTKQLEKDYDVLKRQYDTIKADNDALQAQNQKLQTEILALKNREPTESINLNKETEGSSSNRSENSSDIKLDISTRTQAIDSPLSTQQTSINLFPSSSRPAGVPHQLFQTNSRQDIQCQKIDHMVKEESLSNMFCAMDDQSGLWPWLEQQHFN*