AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1386110


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1386110 MtrunA17_Chr7g0266171 98.776 245 3 0 1 245 1 245 1.12e-177 488
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1386110 AT3G52760.1 69.261 257 67 4 1 245 1 257 5.66e-123 350
Msa1386110 AT2G36300.1 67.681 263 59 5 1 245 1 255 4.59e-115 330

Find 69 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCACAGGGTGGTTTCTT+TGG 0.102268 tig0020787:+19100 Msa1386110:CDS
TGCGGGTCGAACTGGAAATT+TGG 0.234078 tig0020787:+19003 Msa1386110:CDS
TTCCACAGGGTGGTTTCTTT+GGG 0.249145 tig0020787:+19101 Msa1386110:CDS
GGAACCACCACCGCCGATTC+CGG 0.282867 tig0020787:-18723 None:intergenic
GAACCACCACCGCCGATTCC+GGG 0.362735 tig0020787:-18722 None:intergenic
GTATTCATCCGGATCAAATC+TGG 0.368693 tig0020787:+18786 Msa1386110:CDS
AACGCCATATAGAGAAGAAT+TGG 0.368721 tig0020787:-18881 None:intergenic
TGGTCCAATTCTTCTCTATA+TGG 0.369922 tig0020787:+18877 Msa1386110:CDS
CATACTTGTACCAGTGTTGT+TGG 0.376301 tig0020787:+19031 Msa1386110:CDS
AACATGCTTGCGGGTCGAAC+TGG 0.383114 tig0020787:+18995 Msa1386110:CDS
CGGTATGGTTCACGCGAAAC+GGG 0.383392 tig0020787:-18832 None:intergenic
CTCCTCGTCGTCGAAGTTAA+CGG 0.422501 tig0020787:-18744 None:intergenic
TTTGGTTGGAACGGTGGTAT+TGG 0.423341 tig0020787:-18623 None:intergenic
ATGAAAGGGATGCCGGAGTT+TGG 0.440296 tig0020787:-18641 None:intergenic
ACGGTATGGTTCACGCGAAA+CGG 0.443655 tig0020787:-18833 None:intergenic
ATCTACTCTGGTCCCGGAAT+CGG 0.456452 tig0020787:+18710 Msa1386110:CDS
CTTGTGGTTTCTCTTACTGA+TGG 0.457923 tig0020787:+19175 Msa1386110:CDS
GGTCCCGGAATCGGCGGTGG+TGG 0.458366 tig0020787:+18719 Msa1386110:CDS
TGGACCGGAGGAGGTGGAAA+CGG 0.462451 tig0020787:-18687 None:intergenic
CACAAAGACGCTGATCTCTC+TGG 0.477534 tig0020787:+18857 Msa1386110:CDS
GGAGGAGGTGGAAACGGCGG+CGG 0.482053 tig0020787:-18681 None:intergenic
CCGACGGAGGCGAAGGATAC+CGG 0.487942 tig0020787:-18563 None:intergenic
ATTCCGGGACCAGAGTAGAT+TGG 0.488100 tig0020787:-18707 None:intergenic
TGGGCTACTAGAGCTTCAAC+GGG 0.501360 tig0020787:+19151 Msa1386110:CDS
CGATGTCAAATGACATGAAA+GGG 0.504894 tig0020787:-18655 None:intergenic
CCGGTATCCTTCGCCTCCGT+CGG 0.505190 tig0020787:+18563 Msa1386110:CDS
TCTGGTCCCGGAATCGGCGG+TGG 0.508217 tig0020787:+18716 Msa1386110:CDS
GTAGATTGGACCGGAGGAGG+TGG 0.509987 tig0020787:-18693 None:intergenic
GCCGCCGTTTCCACCTCCTC+CGG 0.518829 tig0020787:+18683 Msa1386110:CDS
ATGCCGGAGTTTGGTTGGAA+CGG 0.525630 tig0020787:-18632 None:intergenic
TAGAGCTTCAACGGGGCTTG+TGG 0.525930 tig0020787:+19159 Msa1386110:CDS
GGGGTTACCGACGGAGGCGA+AGG 0.531653 tig0020787:-18570 None:intergenic
AAGGGATGCCGGAGTTTGGT+TGG 0.532020 tig0020787:-18637 None:intergenic
CGACGAGCTCGGTATTCATC+CGG 0.535700 tig0020787:+18775 Msa1386110:CDS
ATACCGAGCTCGTCGAGAAG+AGG 0.537974 tig0020787:-18767 None:intergenic
GGTCCAATCTACTCTGGTCC+CGG 0.538685 tig0020787:+18704 Msa1386110:CDS
CAGCGTCTTTGTGAACGGTA+TGG 0.542374 tig0020787:-18847 None:intergenic
CCTTTCATGTCATTTGACAT+CGG 0.544673 tig0020787:+18656 Msa1386110:CDS
TTGTCCGGCGGGGTTACCGA+CGG 0.546984 tig0020787:-18579 None:intergenic
ATGGGCTACTAGAGCTTCAA+CGG 0.549815 tig0020787:+19150 Msa1386110:CDS
TACTCTGGTCCCGGAATCGG+CGG 0.561190 tig0020787:+18713 Msa1386110:CDS
TGTCTCTTTCAATTACTCGC+CGG 0.564214 tig0020787:+18905 Msa1386110:CDS
GAGCCTCTTCTCGACGAGCT+CGG 0.565975 tig0020787:+18764 Msa1386110:CDS
AAATGACATGAAAGGGATGC+CGG 0.570013 tig0020787:-18648 None:intergenic
GCCTCCGTCGGTAACCCCGC+CGG 0.572655 tig0020787:+18575 Msa1386110:CDS
TCCTCCGGTCCAATCTACTC+TGG 0.574768 tig0020787:+18698 Msa1386110:CDS
AGAGTAGATTGGACCGGAGG+AGG 0.576450 tig0020787:-18696 None:intergenic
CCGGAGTTTGGTTGGAACGG+TGG 0.579512 tig0020787:-18629 None:intergenic
CCACCGTTCCAACCAAACTC+CGG 0.614609 tig0020787:+18629 Msa1386110:CDS
GGGACCAGAGTAGATTGGAC+CGG 0.616175 tig0020787:-18702 None:intergenic
GAGATCAGCGTCTTTGTGAA+CGG 0.616493 tig0020787:-18852 None:intergenic
TGGTTATTCTATGTTGCCAG+TGG 0.618449 tig0020787:+19051 Msa1386110:CDS
GGAACGGTGGTATTGGTACA+CGG 0.619478 tig0020787:-18616 None:intergenic
CCGATGTCAAATGACATGAA+AGG 0.627556 tig0020787:-18656 None:intergenic
GAAAGAATTCAAAGTCCCAC+CGG 0.628112 tig0020787:+18544 Msa1386110:CDS
TCCGGCGGGGTTACCGACGG+AGG 0.630208 tig0020787:-18576 None:intergenic
ACCAGAGTAGATTGGACCGG+AGG 0.633247 tig0020787:-18699 None:intergenic
ACGGAGGCGAAGGATACCGG+TGG 0.636258 tig0020787:-18560 None:intergenic
CATAGAATAACCAACAACAC+TGG 0.637689 tig0020787:-19041 None:intergenic
TTCCGTTAACTTCGACGACG+AGG 0.637706 tig0020787:+18742 Msa1386110:CDS
ATTGCAGAGAAAATCACCAC+TGG 0.645448 tig0020787:-19067 None:intergenic
ACCGGAGGAGGTGGAAACGG+CGG 0.648522 tig0020787:-18684 None:intergenic
AATCTGGAGCAAAATCAGAT+CGG 0.650716 tig0020787:+18802 Msa1386110:CDS
CGGAGGCGAAGGATACCGGT+GGG 0.687481 tig0020787:-18559 None:intergenic
AGCCCAAAGAAACCACCCTG+TGG 0.689092 tig0020787:-19103 None:intergenic
ATGGCTGTGATGAACATCGT+GGG 0.690522 tig0020787:+19194 Msa1386110:CDS
GATGGCTGTGATGAACATCG+TGG 0.761743 tig0020787:+19193 Msa1386110:CDS
GGGCTACTAGAGCTTCAACG+GGG 0.801660 tig0020787:+19152 Msa1386110:CDS
ACGGTGGTATTGGTACACGG+CGG 0.809815 tig0020787:-18613 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCAATTCGGTGTGATTTT+AGG + tig0020787:18932-18951 Msa1386110:CDS 30.0%
CGATGTCAAATGACATGAAA+GGG - tig0020787:18658-18677 None:intergenic 35.0%
! CCTTTCATGTCATTTGACAT+CGG + tig0020787:18656-18675 Msa1386110:CDS 35.0%
! ATTTTGCTCCAGATTTGATC+CGG - tig0020787:18797-18816 None:intergenic 35.0%
! AATCTGGAGCAAAATCAGAT+CGG + tig0020787:18802-18821 Msa1386110:CDS 35.0%
TGGTCCAATTCTTCTCTATA+TGG + tig0020787:18877-18896 Msa1386110:CDS 35.0%
AACGCCATATAGAGAAGAAT+TGG - tig0020787:18884-18903 None:intergenic 35.0%
! CACACCGAATTGAATTTTTC+CGG - tig0020787:18927-18946 None:intergenic 35.0%
!!! AATTCGGTGTGATTTTAGGT+TGG + tig0020787:18936-18955 Msa1386110:CDS 35.0%
CATAGAATAACCAACAACAC+TGG - tig0020787:19044-19063 None:intergenic 35.0%
!!! AATATCGCTTTTTCTTCCAC+AGG + tig0020787:19087-19106 Msa1386110:CDS 35.0%
!!! ATATCGCTTTTTCTTCCACA+GGG + tig0020787:19088-19107 Msa1386110:CDS 35.0%
GAAAGAATTCAAAGTCCCAC+CGG + tig0020787:18544-18563 Msa1386110:CDS 40.0%
!! TTTTGAAGATTTTGTCCGGC+GGG - tig0020787:18593-18612 None:intergenic 40.0%
!!! GTTTTGAAGATTTTGTCCGG+CGG - tig0020787:18594-18613 None:intergenic 40.0%
!!! GCGGTTTTGAAGATTTTGTC+CGG - tig0020787:18597-18616 None:intergenic 40.0%
AAATGACATGAAAGGGATGC+CGG - tig0020787:18651-18670 None:intergenic 40.0%
CCGATGTCAAATGACATGAA+AGG - tig0020787:18659-18678 None:intergenic 40.0%
GTATTCATCCGGATCAAATC+TGG + tig0020787:18786-18805 Msa1386110:CDS 40.0%
TGTCTCTTTCAATTACTCGC+CGG + tig0020787:18905-18924 Msa1386110:CDS 40.0%
CTCGCCGGAAAAATTCAATT+CGG + tig0020787:18920-18939 Msa1386110:CDS 40.0%
!!! TGTGATTTTAGGTTGGATCG+TGG + tig0020787:18943-18962 Msa1386110:CDS 40.0%
!! CGTTGTTTTCAACATGCTTG+CGG + tig0020787:18985-19004 Msa1386110:CDS 40.0%
!! GTTGTTTTCAACATGCTTGC+GGG + tig0020787:18986-19005 Msa1386110:CDS 40.0%
CATACTTGTACCAGTGTTGT+TGG + tig0020787:19031-19050 Msa1386110:CDS 40.0%
TGGTTATTCTATGTTGCCAG+TGG + tig0020787:19051-19070 Msa1386110:CDS 40.0%
ATTGCAGAGAAAATCACCAC+TGG - tig0020787:19070-19089 None:intergenic 40.0%
! TCGCTGCGATTTTTGTTCTA+TGG + tig0020787:19131-19150 Msa1386110:CDS 40.0%
! CGCTGCGATTTTTGTTCTAT+GGG + tig0020787:19132-19151 Msa1386110:CDS 40.0%
CTTGTGGTTTCTCTTACTGA+TGG + tig0020787:19175-19194 Msa1386110:CDS 40.0%
! TTTGAAGATTTTGTCCGGCG+GGG - tig0020787:18592-18611 None:intergenic 45.0%
TTTGGTTGGAACGGTGGTAT+TGG - tig0020787:18626-18645 None:intergenic 45.0%
GAGATCAGCGTCTTTGTGAA+CGG - tig0020787:18855-18874 None:intergenic 45.0%
TTCCACAGGGTGGTTTCTTT+GGG + tig0020787:19101-19120 Msa1386110:CDS 45.0%
! ATGGGCTACTAGAGCTTCAA+CGG + tig0020787:19150-19169 Msa1386110:CDS 45.0%
ATGGCTGTGATGAACATCGT+GGG + tig0020787:19194-19213 Msa1386110:CDS 45.0%
!! GGAACGGTGGTATTGGTACA+CGG - tig0020787:18619-18638 None:intergenic 50.0%
ATGCCGGAGTTTGGTTGGAA+CGG - tig0020787:18635-18654 None:intergenic 50.0%
ATGAAAGGGATGCCGGAGTT+TGG - tig0020787:18644-18663 None:intergenic 50.0%
ATTCCGGGACCAGAGTAGAT+TGG - tig0020787:18710-18729 None:intergenic 50.0%
ATCTACTCTGGTCCCGGAAT+CGG + tig0020787:18710-18729 Msa1386110:CDS 50.0%
TTCCGTTAACTTCGACGACG+AGG + tig0020787:18742-18761 Msa1386110:CDS 50.0%
CTCCTCGTCGTCGAAGTTAA+CGG - tig0020787:18747-18766 None:intergenic 50.0%
ACGGTATGGTTCACGCGAAA+CGG - tig0020787:18836-18855 None:intergenic 50.0%
CAGCGTCTTTGTGAACGGTA+TGG - tig0020787:18850-18869 None:intergenic 50.0%
CACAAAGACGCTGATCTCTC+TGG + tig0020787:18857-18876 Msa1386110:CDS 50.0%
TGCGGGTCGAACTGGAAATT+TGG + tig0020787:19003-19022 Msa1386110:CDS 50.0%
!!! TCGCTTTTTCTTCCACAGGG+TGG + tig0020787:19091-19110 Msa1386110:CDS 50.0%
CTTCCACAGGGTGGTTTCTT+TGG + tig0020787:19100-19119 Msa1386110:CDS 50.0%
TGGGCTACTAGAGCTTCAAC+GGG + tig0020787:19151-19170 Msa1386110:CDS 50.0%
GATGGCTGTGATGAACATCG+TGG + tig0020787:19193-19212 Msa1386110:CDS 50.0%
!! ACGGTGGTATTGGTACACGG+CGG - tig0020787:18616-18635 None:intergenic 55.0%
CCACCGTTCCAACCAAACTC+CGG + tig0020787:18629-18648 Msa1386110:CDS 55.0%
AAGGGATGCCGGAGTTTGGT+TGG - tig0020787:18640-18659 None:intergenic 55.0%
AGAGTAGATTGGACCGGAGG+AGG - tig0020787:18699-18718 None:intergenic 55.0%
TCCTCCGGTCCAATCTACTC+TGG + tig0020787:18698-18717 Msa1386110:CDS 55.0%
ACCAGAGTAGATTGGACCGG+AGG - tig0020787:18702-18721 None:intergenic 55.0%
GGGACCAGAGTAGATTGGAC+CGG - tig0020787:18705-18724 None:intergenic 55.0%
GGTCCAATCTACTCTGGTCC+CGG + tig0020787:18704-18723 Msa1386110:CDS 55.0%
ATACCGAGCTCGTCGAGAAG+AGG - tig0020787:18770-18789 None:intergenic 55.0%
CGACGAGCTCGGTATTCATC+CGG + tig0020787:18775-18794 Msa1386110:CDS 55.0%
CGGTATGGTTCACGCGAAAC+GGG - tig0020787:18835-18854 None:intergenic 55.0%
AACATGCTTGCGGGTCGAAC+TGG + tig0020787:18995-19014 Msa1386110:CDS 55.0%
AGCCCAAAGAAACCACCCTG+TGG - tig0020787:19106-19125 None:intergenic 55.0%
GGGCTACTAGAGCTTCAACG+GGG + tig0020787:19152-19171 Msa1386110:CDS 55.0%
TAGAGCTTCAACGGGGCTTG+TGG + tig0020787:19159-19178 Msa1386110:CDS 55.0%
CCGGAGTTTGGTTGGAACGG+TGG - tig0020787:18632-18651 None:intergenic 60.0%
TGGACCGGAGGAGGTGGAAA+CGG - tig0020787:18690-18709 None:intergenic 60.0%
GTAGATTGGACCGGAGGAGG+TGG - tig0020787:18696-18715 None:intergenic 60.0%
TACTCTGGTCCCGGAATCGG+CGG + tig0020787:18713-18732 Msa1386110:CDS 60.0%
GAGCCTCTTCTCGACGAGCT+CGG + tig0020787:18764-18783 Msa1386110:CDS 60.0%
CGGAGGCGAAGGATACCGGT+GGG - tig0020787:18562-18581 None:intergenic 65.0%
ACGGAGGCGAAGGATACCGG+TGG - tig0020787:18563-18582 None:intergenic 65.0%
CCGACGGAGGCGAAGGATAC+CGG - tig0020787:18566-18585 None:intergenic 65.0%
CCGGTATCCTTCGCCTCCGT+CGG + tig0020787:18563-18582 Msa1386110:CDS 65.0%
TTGTCCGGCGGGGTTACCGA+CGG - tig0020787:18582-18601 None:intergenic 65.0%
ACCGGAGGAGGTGGAAACGG+CGG - tig0020787:18687-18706 None:intergenic 65.0%
!! GAACCACCACCGCCGATTCC+GGG - tig0020787:18725-18744 None:intergenic 65.0%
!! GGAACCACCACCGCCGATTC+CGG - tig0020787:18726-18745 None:intergenic 65.0%
GGGGTTACCGACGGAGGCGA+AGG - tig0020787:18573-18592 None:intergenic 70.0%
GGAGGAGGTGGAAACGGCGG+CGG - tig0020787:18684-18703 None:intergenic 70.0%
GCCGCCGTTTCCACCTCCTC+CGG + tig0020787:18683-18702 Msa1386110:CDS 70.0%
TCTGGTCCCGGAATCGGCGG+TGG + tig0020787:18716-18735 Msa1386110:CDS 70.0%
GCCTCCGTCGGTAACCCCGC+CGG + tig0020787:18575-18594 Msa1386110:CDS 75.0%
TCCGGCGGGGTTACCGACGG+AGG - tig0020787:18579-18598 None:intergenic 75.0%
GGTCCCGGAATCGGCGGTGG+TGG + tig0020787:18719-18738 Msa1386110:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0020787 gene 18539 19276 18539 ID=Msa1386110;Name=Msa1386110
tig0020787 mRNA 18539 19276 18539 ID=Msa1386110-mRNA-1;Parent=Msa1386110;Name=Msa1386110-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|245
tig0020787 exon 18539 19276 18539 ID=Msa1386110-mRNA-1:exon:9993;Parent=Msa1386110-mRNA-1
tig0020787 CDS 18539 19276 18539 ID=Msa1386110-mRNA-1:cds;Parent=Msa1386110-mRNA-1
Gene Sequence

>Msa1386110

ATGGAGAAAGAATTCAAAGTCCCACCGGTATCCTTCGCCTCCGTCGGTAACCCCGCCGGACAAAATCTTCAAAACCGCCGTGTACCAATACCACCGTTCCAACCAAACTCCGGCATCCCTTTCATGTCATTTGACATCGGCTCCGCCGCCGTTTCCACCTCCTCCGGTCCAATCTACTCTGGTCCCGGAATCGGCGGTGGTGGTTCCGTTAACTTCGACGACGAGGAGCCTCTTCTCGACGAGCTCGGTATTCATCCGGATCAAATCTGGAGCAAAATCAGATCGGTTCTTAACCCGTTTCGCGTGAACCATACCGTTCACAAAGACGCTGATCTCTCTGGTCCAATTCTTCTCTATATGGCGTTTTGTCTCTTTCAATTACTCGCCGGAAAAATTCAATTCGGTGTGATTTTAGGTTGGATCGTGGTTTCTTCGATCTTTTTGTACGTTGTTTTCAACATGCTTGCGGGTCGAACTGGAAATTTGGATCTACATACTTGTACCAGTGTTGTTGGTTATTCTATGTTGCCAGTGGTGATTTTCTCTGCAATATCGCTTTTTCTTCCACAGGGTGGTTTCTTTGGGCTTGCTATCGCTGCGATTTTTGTTCTATGGGCTACTAGAGCTTCAACGGGGCTTGTGGTTTCTCTTACTGATGGCTGTGATGAACATCGTGGGTTGATAGCGTACGCGTGTTTCTTGATTTACACTCTGTTTTCGTTGCTTGTGATTTTCTAG

Protein sequence

>Msa1386110

MEKEFKVPPVSFASVGNPAGQNLQNRRVPIPPFQPNSGIPFMSFDIGSAAVSTSSGPIYSGPGIGGGGSVNFDDEEPLLDELGIHPDQIWSKIRSVLNPFRVNHTVHKDADLSGPILLYMAFCLFQLLAGKIQFGVILGWIVVSSIFLYVVFNMLAGRTGNLDLHTCTSVVGYSMLPVVIFSAISLFLPQGGFFGLAIAAIFVLWATRASTGLVVSLTDGCDEHRGLIAYACFLIYTLFSLLVIF*