AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1427660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1427660 MtrunA17_Chr7g0266171 98.776 245 3 0 1 245 1 245 1.12e-177 488
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1427660 AT3G52760.1 69.261 257 67 4 1 245 1 257 5.66e-123 350
Msa1427660 AT2G36300.1 67.681 263 59 5 1 245 1 255 4.59e-115 330

Find 69 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCACAGGGTGGTTTCTT+TGG 0.102268 tig0026197:+39541 Msa1427660:CDS
TGCGGGTCGAACTGGAAATT+TGG 0.234078 tig0026197:+39444 Msa1427660:CDS
TTCCACAGGGTGGTTTCTTT+GGG 0.249145 tig0026197:+39542 Msa1427660:CDS
GGAACCACCACCGCCGATTC+CGG 0.282867 tig0026197:-39164 None:intergenic
GAACCACCACCGCCGATTCC+GGG 0.362735 tig0026197:-39163 None:intergenic
GTATTCATCCGGATCAAATC+TGG 0.368693 tig0026197:+39227 Msa1427660:CDS
AACGCCATATAGAGAAGAAT+TGG 0.368721 tig0026197:-39322 None:intergenic
TGGTCCAATTCTTCTCTATA+TGG 0.369922 tig0026197:+39318 Msa1427660:CDS
CATACTTGTACCAGTGTTGT+TGG 0.376301 tig0026197:+39472 Msa1427660:CDS
AACATGCTTGCGGGTCGAAC+TGG 0.383114 tig0026197:+39436 Msa1427660:CDS
CGGTATGGTTCACGCGAAAC+GGG 0.383392 tig0026197:-39273 None:intergenic
CTCCTCGTCGTCGAAGTTAA+CGG 0.422501 tig0026197:-39185 None:intergenic
TTTGGTTGGAACGGTGGTAT+TGG 0.423341 tig0026197:-39064 None:intergenic
ATGAAAGGGATGCCGGAGTT+TGG 0.440296 tig0026197:-39082 None:intergenic
ACGGTATGGTTCACGCGAAA+CGG 0.443655 tig0026197:-39274 None:intergenic
ATCTACTCTGGTCCCGGAAT+CGG 0.456452 tig0026197:+39151 Msa1427660:CDS
CTTGTGGTTTCTCTTACTGA+TGG 0.457923 tig0026197:+39616 Msa1427660:CDS
GGTCCCGGAATCGGCGGTGG+TGG 0.458366 tig0026197:+39160 Msa1427660:CDS
TGGACCGGAGGAGGTGGAAA+CGG 0.462451 tig0026197:-39128 None:intergenic
CACAAAGACGCTGATCTCTC+TGG 0.477534 tig0026197:+39298 Msa1427660:CDS
GGAGGAGGTGGAAACGGCGG+CGG 0.482053 tig0026197:-39122 None:intergenic
CCGACGGAGGCGAAGGATAC+CGG 0.487942 tig0026197:-39004 None:intergenic
ATTCCGGGACCAGAGTAGAT+TGG 0.488100 tig0026197:-39148 None:intergenic
TGGGCTACTAGAGCTTCAAC+GGG 0.501360 tig0026197:+39592 Msa1427660:CDS
CGATGTCAAATGACATGAAA+GGG 0.504894 tig0026197:-39096 None:intergenic
CCGGTATCCTTCGCCTCCGT+CGG 0.505190 tig0026197:+39004 Msa1427660:CDS
TCTGGTCCCGGAATCGGCGG+TGG 0.508217 tig0026197:+39157 Msa1427660:CDS
GTAGATTGGACCGGAGGAGG+TGG 0.509987 tig0026197:-39134 None:intergenic
GCCGCCGTTTCCACCTCCTC+CGG 0.518829 tig0026197:+39124 Msa1427660:CDS
ATGCCGGAGTTTGGTTGGAA+CGG 0.525630 tig0026197:-39073 None:intergenic
TAGAGCTTCAACGGGGCTTG+TGG 0.525930 tig0026197:+39600 Msa1427660:CDS
GGGGTTACCGACGGAGGCGA+AGG 0.531653 tig0026197:-39011 None:intergenic
AAGGGATGCCGGAGTTTGGT+TGG 0.532020 tig0026197:-39078 None:intergenic
CGACGAGCTCGGTATTCATC+CGG 0.535700 tig0026197:+39216 Msa1427660:CDS
ATACCGAGCTCGTCGAGAAG+AGG 0.537974 tig0026197:-39208 None:intergenic
GGTCCAATCTACTCTGGTCC+CGG 0.538685 tig0026197:+39145 Msa1427660:CDS
CAGCGTCTTTGTGAACGGTA+TGG 0.542374 tig0026197:-39288 None:intergenic
CCTTTCATGTCATTTGACAT+CGG 0.544673 tig0026197:+39097 Msa1427660:CDS
TTGTCCGGCGGGGTTACCGA+CGG 0.546984 tig0026197:-39020 None:intergenic
ATGGGCTACTAGAGCTTCAA+CGG 0.549815 tig0026197:+39591 Msa1427660:CDS
TACTCTGGTCCCGGAATCGG+CGG 0.561190 tig0026197:+39154 Msa1427660:CDS
TGTCTCTTTCAATTACTCGC+CGG 0.564214 tig0026197:+39346 Msa1427660:CDS
GAGCCTCTTCTCGACGAGCT+CGG 0.565975 tig0026197:+39205 Msa1427660:CDS
AAATGACATGAAAGGGATGC+CGG 0.570013 tig0026197:-39089 None:intergenic
GCCTCCGTCGGTAACCCCGC+CGG 0.572655 tig0026197:+39016 Msa1427660:CDS
TCCTCCGGTCCAATCTACTC+TGG 0.574768 tig0026197:+39139 Msa1427660:CDS
AGAGTAGATTGGACCGGAGG+AGG 0.576450 tig0026197:-39137 None:intergenic
CCGGAGTTTGGTTGGAACGG+TGG 0.579512 tig0026197:-39070 None:intergenic
CCACCGTTCCAACCAAACTC+CGG 0.614609 tig0026197:+39070 Msa1427660:CDS
GGGACCAGAGTAGATTGGAC+CGG 0.616175 tig0026197:-39143 None:intergenic
GAGATCAGCGTCTTTGTGAA+CGG 0.616493 tig0026197:-39293 None:intergenic
TGGTTATTCTATGTTGCCAG+TGG 0.618449 tig0026197:+39492 Msa1427660:CDS
GGAACGGTGGTATTGGTACA+CGG 0.619478 tig0026197:-39057 None:intergenic
CCGATGTCAAATGACATGAA+AGG 0.627556 tig0026197:-39097 None:intergenic
GAAAGAATTCAAAGTCCCAC+CGG 0.628112 tig0026197:+38985 Msa1427660:CDS
TCCGGCGGGGTTACCGACGG+AGG 0.630208 tig0026197:-39017 None:intergenic
ACCAGAGTAGATTGGACCGG+AGG 0.633247 tig0026197:-39140 None:intergenic
ACGGAGGCGAAGGATACCGG+TGG 0.636258 tig0026197:-39001 None:intergenic
CATAGAATAACCAACAACAC+TGG 0.637689 tig0026197:-39482 None:intergenic
TTCCGTTAACTTCGACGACG+AGG 0.637706 tig0026197:+39183 Msa1427660:CDS
ATTGCAGAGAAAATCACCAC+TGG 0.645448 tig0026197:-39508 None:intergenic
ACCGGAGGAGGTGGAAACGG+CGG 0.648522 tig0026197:-39125 None:intergenic
AATCTGGAGCAAAATCAGAT+CGG 0.650716 tig0026197:+39243 Msa1427660:CDS
CGGAGGCGAAGGATACCGGT+GGG 0.687481 tig0026197:-39000 None:intergenic
AGCCCAAAGAAACCACCCTG+TGG 0.689092 tig0026197:-39544 None:intergenic
ATGGCTGTGATGAACATCGT+GGG 0.690522 tig0026197:+39635 Msa1427660:CDS
GATGGCTGTGATGAACATCG+TGG 0.761743 tig0026197:+39634 Msa1427660:CDS
GGGCTACTAGAGCTTCAACG+GGG 0.801660 tig0026197:+39593 Msa1427660:CDS
ACGGTGGTATTGGTACACGG+CGG 0.809815 tig0026197:-39054 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCAATTCGGTGTGATTTT+AGG + tig0026197:39373-39392 Msa1427660:CDS 30.0%
CGATGTCAAATGACATGAAA+GGG - tig0026197:39099-39118 None:intergenic 35.0%
! CCTTTCATGTCATTTGACAT+CGG + tig0026197:39097-39116 Msa1427660:CDS 35.0%
! ATTTTGCTCCAGATTTGATC+CGG - tig0026197:39238-39257 None:intergenic 35.0%
! AATCTGGAGCAAAATCAGAT+CGG + tig0026197:39243-39262 Msa1427660:CDS 35.0%
TGGTCCAATTCTTCTCTATA+TGG + tig0026197:39318-39337 Msa1427660:CDS 35.0%
AACGCCATATAGAGAAGAAT+TGG - tig0026197:39325-39344 None:intergenic 35.0%
! CACACCGAATTGAATTTTTC+CGG - tig0026197:39368-39387 None:intergenic 35.0%
!!! AATTCGGTGTGATTTTAGGT+TGG + tig0026197:39377-39396 Msa1427660:CDS 35.0%
CATAGAATAACCAACAACAC+TGG - tig0026197:39485-39504 None:intergenic 35.0%
!!! AATATCGCTTTTTCTTCCAC+AGG + tig0026197:39528-39547 Msa1427660:CDS 35.0%
!!! ATATCGCTTTTTCTTCCACA+GGG + tig0026197:39529-39548 Msa1427660:CDS 35.0%
GAAAGAATTCAAAGTCCCAC+CGG + tig0026197:38985-39004 Msa1427660:CDS 40.0%
!! TTTTGAAGATTTTGTCCGGC+GGG - tig0026197:39034-39053 None:intergenic 40.0%
!!! GTTTTGAAGATTTTGTCCGG+CGG - tig0026197:39035-39054 None:intergenic 40.0%
!!! GCGGTTTTGAAGATTTTGTC+CGG - tig0026197:39038-39057 None:intergenic 40.0%
AAATGACATGAAAGGGATGC+CGG - tig0026197:39092-39111 None:intergenic 40.0%
CCGATGTCAAATGACATGAA+AGG - tig0026197:39100-39119 None:intergenic 40.0%
GTATTCATCCGGATCAAATC+TGG + tig0026197:39227-39246 Msa1427660:CDS 40.0%
TGTCTCTTTCAATTACTCGC+CGG + tig0026197:39346-39365 Msa1427660:CDS 40.0%
CTCGCCGGAAAAATTCAATT+CGG + tig0026197:39361-39380 Msa1427660:CDS 40.0%
!!! TGTGATTTTAGGTTGGATCG+TGG + tig0026197:39384-39403 Msa1427660:CDS 40.0%
!! CGTTGTTTTCAACATGCTTG+CGG + tig0026197:39426-39445 Msa1427660:CDS 40.0%
!! GTTGTTTTCAACATGCTTGC+GGG + tig0026197:39427-39446 Msa1427660:CDS 40.0%
CATACTTGTACCAGTGTTGT+TGG + tig0026197:39472-39491 Msa1427660:CDS 40.0%
TGGTTATTCTATGTTGCCAG+TGG + tig0026197:39492-39511 Msa1427660:CDS 40.0%
ATTGCAGAGAAAATCACCAC+TGG - tig0026197:39511-39530 None:intergenic 40.0%
! TCGCTGCGATTTTTGTTCTA+TGG + tig0026197:39572-39591 Msa1427660:CDS 40.0%
! CGCTGCGATTTTTGTTCTAT+GGG + tig0026197:39573-39592 Msa1427660:CDS 40.0%
CTTGTGGTTTCTCTTACTGA+TGG + tig0026197:39616-39635 Msa1427660:CDS 40.0%
! TTTGAAGATTTTGTCCGGCG+GGG - tig0026197:39033-39052 None:intergenic 45.0%
TTTGGTTGGAACGGTGGTAT+TGG - tig0026197:39067-39086 None:intergenic 45.0%
GAGATCAGCGTCTTTGTGAA+CGG - tig0026197:39296-39315 None:intergenic 45.0%
TTCCACAGGGTGGTTTCTTT+GGG + tig0026197:39542-39561 Msa1427660:CDS 45.0%
! ATGGGCTACTAGAGCTTCAA+CGG + tig0026197:39591-39610 Msa1427660:CDS 45.0%
ATGGCTGTGATGAACATCGT+GGG + tig0026197:39635-39654 Msa1427660:CDS 45.0%
!! GGAACGGTGGTATTGGTACA+CGG - tig0026197:39060-39079 None:intergenic 50.0%
ATGCCGGAGTTTGGTTGGAA+CGG - tig0026197:39076-39095 None:intergenic 50.0%
ATGAAAGGGATGCCGGAGTT+TGG - tig0026197:39085-39104 None:intergenic 50.0%
ATTCCGGGACCAGAGTAGAT+TGG - tig0026197:39151-39170 None:intergenic 50.0%
ATCTACTCTGGTCCCGGAAT+CGG + tig0026197:39151-39170 Msa1427660:CDS 50.0%
TTCCGTTAACTTCGACGACG+AGG + tig0026197:39183-39202 Msa1427660:CDS 50.0%
CTCCTCGTCGTCGAAGTTAA+CGG - tig0026197:39188-39207 None:intergenic 50.0%
ACGGTATGGTTCACGCGAAA+CGG - tig0026197:39277-39296 None:intergenic 50.0%
CAGCGTCTTTGTGAACGGTA+TGG - tig0026197:39291-39310 None:intergenic 50.0%
CACAAAGACGCTGATCTCTC+TGG + tig0026197:39298-39317 Msa1427660:CDS 50.0%
TGCGGGTCGAACTGGAAATT+TGG + tig0026197:39444-39463 Msa1427660:CDS 50.0%
!!! TCGCTTTTTCTTCCACAGGG+TGG + tig0026197:39532-39551 Msa1427660:CDS 50.0%
CTTCCACAGGGTGGTTTCTT+TGG + tig0026197:39541-39560 Msa1427660:CDS 50.0%
TGGGCTACTAGAGCTTCAAC+GGG + tig0026197:39592-39611 Msa1427660:CDS 50.0%
GATGGCTGTGATGAACATCG+TGG + tig0026197:39634-39653 Msa1427660:CDS 50.0%
!! ACGGTGGTATTGGTACACGG+CGG - tig0026197:39057-39076 None:intergenic 55.0%
CCACCGTTCCAACCAAACTC+CGG + tig0026197:39070-39089 Msa1427660:CDS 55.0%
AAGGGATGCCGGAGTTTGGT+TGG - tig0026197:39081-39100 None:intergenic 55.0%
AGAGTAGATTGGACCGGAGG+AGG - tig0026197:39140-39159 None:intergenic 55.0%
TCCTCCGGTCCAATCTACTC+TGG + tig0026197:39139-39158 Msa1427660:CDS 55.0%
ACCAGAGTAGATTGGACCGG+AGG - tig0026197:39143-39162 None:intergenic 55.0%
GGGACCAGAGTAGATTGGAC+CGG - tig0026197:39146-39165 None:intergenic 55.0%
GGTCCAATCTACTCTGGTCC+CGG + tig0026197:39145-39164 Msa1427660:CDS 55.0%
ATACCGAGCTCGTCGAGAAG+AGG - tig0026197:39211-39230 None:intergenic 55.0%
CGACGAGCTCGGTATTCATC+CGG + tig0026197:39216-39235 Msa1427660:CDS 55.0%
CGGTATGGTTCACGCGAAAC+GGG - tig0026197:39276-39295 None:intergenic 55.0%
AACATGCTTGCGGGTCGAAC+TGG + tig0026197:39436-39455 Msa1427660:CDS 55.0%
AGCCCAAAGAAACCACCCTG+TGG - tig0026197:39547-39566 None:intergenic 55.0%
GGGCTACTAGAGCTTCAACG+GGG + tig0026197:39593-39612 Msa1427660:CDS 55.0%
TAGAGCTTCAACGGGGCTTG+TGG + tig0026197:39600-39619 Msa1427660:CDS 55.0%
CCGGAGTTTGGTTGGAACGG+TGG - tig0026197:39073-39092 None:intergenic 60.0%
TGGACCGGAGGAGGTGGAAA+CGG - tig0026197:39131-39150 None:intergenic 60.0%
GTAGATTGGACCGGAGGAGG+TGG - tig0026197:39137-39156 None:intergenic 60.0%
TACTCTGGTCCCGGAATCGG+CGG + tig0026197:39154-39173 Msa1427660:CDS 60.0%
GAGCCTCTTCTCGACGAGCT+CGG + tig0026197:39205-39224 Msa1427660:CDS 60.0%
CGGAGGCGAAGGATACCGGT+GGG - tig0026197:39003-39022 None:intergenic 65.0%
ACGGAGGCGAAGGATACCGG+TGG - tig0026197:39004-39023 None:intergenic 65.0%
CCGACGGAGGCGAAGGATAC+CGG - tig0026197:39007-39026 None:intergenic 65.0%
CCGGTATCCTTCGCCTCCGT+CGG + tig0026197:39004-39023 Msa1427660:CDS 65.0%
TTGTCCGGCGGGGTTACCGA+CGG - tig0026197:39023-39042 None:intergenic 65.0%
ACCGGAGGAGGTGGAAACGG+CGG - tig0026197:39128-39147 None:intergenic 65.0%
!! GAACCACCACCGCCGATTCC+GGG - tig0026197:39166-39185 None:intergenic 65.0%
!! GGAACCACCACCGCCGATTC+CGG - tig0026197:39167-39186 None:intergenic 65.0%
GGGGTTACCGACGGAGGCGA+AGG - tig0026197:39014-39033 None:intergenic 70.0%
GGAGGAGGTGGAAACGGCGG+CGG - tig0026197:39125-39144 None:intergenic 70.0%
GCCGCCGTTTCCACCTCCTC+CGG + tig0026197:39124-39143 Msa1427660:CDS 70.0%
TCTGGTCCCGGAATCGGCGG+TGG + tig0026197:39157-39176 Msa1427660:CDS 70.0%
GCCTCCGTCGGTAACCCCGC+CGG + tig0026197:39016-39035 Msa1427660:CDS 75.0%
TCCGGCGGGGTTACCGACGG+AGG - tig0026197:39020-39039 None:intergenic 75.0%
GGTCCCGGAATCGGCGGTGG+TGG + tig0026197:39160-39179 Msa1427660:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0026197 gene 38980 39717 38980 ID=Msa1427660;Name=Msa1427660
tig0026197 mRNA 38980 39717 38980 ID=Msa1427660-mRNA-1;Parent=Msa1427660;Name=Msa1427660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|245
tig0026197 exon 38980 39717 38980 ID=Msa1427660-mRNA-1:exon:5548;Parent=Msa1427660-mRNA-1
tig0026197 CDS 38980 39717 38980 ID=Msa1427660-mRNA-1:cds;Parent=Msa1427660-mRNA-1
Gene Sequence

>Msa1427660

ATGGAGAAAGAATTCAAAGTCCCACCGGTATCCTTCGCCTCCGTCGGTAACCCCGCCGGACAAAATCTTCAAAACCGCCGTGTACCAATACCACCGTTCCAACCAAACTCCGGCATCCCTTTCATGTCATTTGACATCGGCTCCGCCGCCGTTTCCACCTCCTCCGGTCCAATCTACTCTGGTCCCGGAATCGGCGGTGGTGGTTCCGTTAACTTCGACGACGAGGAGCCTCTTCTCGACGAGCTCGGTATTCATCCGGATCAAATCTGGAGCAAAATCAGATCGGTTCTTAACCCGTTTCGCGTGAACCATACCGTTCACAAAGACGCTGATCTCTCTGGTCCAATTCTTCTCTATATGGCGTTTTGTCTCTTTCAATTACTCGCCGGAAAAATTCAATTCGGTGTGATTTTAGGTTGGATCGTGGTTTCTTCGATCTTTTTGTACGTTGTTTTCAACATGCTTGCGGGTCGAACTGGAAATTTGGATCTACATACTTGTACCAGTGTTGTTGGTTATTCTATGTTGCCAGTGGTGATTTTCTCTGCAATATCGCTTTTTCTTCCACAGGGTGGTTTCTTTGGGCTTGCTATCGCTGCGATTTTTGTTCTATGGGCTACTAGAGCTTCAACGGGGCTTGTGGTTTCTCTTACTGATGGCTGTGATGAACATCGTGGGTTGATAGCGTACGCGTGTTTCTTGATTTACACTCTGTTTTCGTTGCTTGTGATTTTCTAG

Protein sequence

>Msa1427660

MEKEFKVPPVSFASVGNPAGQNLQNRRVPIPPFQPNSGIPFMSFDIGSAAVSTSSGPIYSGPGIGGGGSVNFDDEEPLLDELGIHPDQIWSKIRSVLNPFRVNHTVHKDADLSGPILLYMAFCLFQLLAGKIQFGVILGWIVVSSIFLYVVFNMLAGRTGNLDLHTCTSVVGYSMLPVVIFSAISLFLPQGGFFGLAIAAIFVLWATRASTGLVVSLTDGCDEHRGLIAYACFLIYTLFSLLVIF*