AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003370.t1 MTR_3g106420 96.050 481 18 1 1 481 1 480 0.0 938
MS.gene003370.t1 MTR_3g106480 91.858 479 38 1 3 481 1 478 0.0 897
MS.gene003370.t1 MTR_3g106485 93.528 479 26 2 3 481 1 474 0.0 883
MS.gene003370.t1 MTR_1g083190 92.723 481 26 1 1 481 1 472 0.0 876
MS.gene003370.t1 MTR_3g106430 91.841 478 34 2 3 480 1 473 0.0 838
MS.gene003370.t1 MTR_1g073400 89.562 479 45 1 3 481 1 474 0.0 830
MS.gene003370.t1 MTR_1g115145 87.292 480 53 4 3 481 1 473 0.0 825
MS.gene003370.t1 MTR_1g071130 84.551 479 44 2 3 481 1 449 0.0 824
MS.gene003370.t1 MTR_3g106470 83.891 478 20 3 3 480 32 452 0.0 765
MS.gene003370.t1 MTR_8g063830 79.828 466 52 5 3 467 1 425 0.0 669
MS.gene003370.t1 MTR_3g065540 64.348 115 14 1 42 156 14 101 3.58e-40 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003370.t1 AT5G25260 72.689 476 115 3 3 477 1 462 0.0 717
MS.gene003370.t1 AT5G25250 72.574 474 115 2 3 476 1 459 0.0 714
MS.gene003370.t1 AT5G64870 69.979 473 130 2 4 476 3 463 0.0 650

Find 117 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGAGTATTTCTCATATT+TGG 0.101480 4.1:-28027944 MS.gene003370:CDS
TTGAGCCTAAGATTAGTATT+TGG 0.251063 4.1:-28026525 MS.gene003370:CDS
CATGAGTATTTCTCATATTT+GGG 0.284347 4.1:-28027943 MS.gene003370:CDS
CGAAGAAGGCTGTGGCTCTA+AGG 0.317541 4.1:-28027615 MS.gene003370:CDS
GTCAACCCTAGCTTGATTCT+TGG 0.318930 4.1:+28027900 None:intergenic
GCTAAGATGAAAGGTGAAAT+TGG 0.319472 4.1:-28027868 MS.gene003370:CDS
CAATGCAAATGTTAAGCAAT+TGG 0.339200 4.1:-28027980 MS.gene003370:CDS
AGGGATTACTTGATGATAAA+TGG 0.347159 4.1:-28026598 MS.gene003370:CDS
CATCAAGAATGAAAATTTAC+CGG 0.347355 4.1:-28028428 None:intergenic
CTCCCCGCTGTCTTCACAAT+TGG 0.348065 4.1:-28028234 MS.gene003370:CDS
CATGTTGCCTCCTGCTTGGA+TGG 0.350630 4.1:-28026383 MS.gene003370:CDS
ACTTCGAATGTGTAGTTTAC+CGG 0.360846 4.1:+28028291 None:intergenic
CAGCTTGAGTTGAATCAATT+CGG 0.364537 4.1:-28028015 MS.gene003370:CDS
GTGAAATTGGTTCAAAATTG+AGG 0.384625 4.1:-28027855 MS.gene003370:CDS
TTAGTCCAAATACTAATCTT+AGG 0.384650 4.1:+28026520 None:intergenic
AGAGCTATACGCAAAGAAAA+AGG 0.384802 4.1:-28026710 MS.gene003370:CDS
AAGATTAGTATTTGGACTAA+TGG 0.384982 4.1:-28026517 MS.gene003370:CDS
TCAGAATACCTTGTGATAAC+AGG 0.405430 4.1:-28028393 MS.gene003370:CDS
TGTAGGTACAAGAAGCAAAC+TGG 0.406248 4.1:-28026852 MS.gene003370:intron
TGGTCCTCGTGTTGACGATC+AGG 0.407219 4.1:-28028214 MS.gene003370:CDS
GGCTTGGACCAAGGCGGCTC+AGG 0.413477 4.1:-28027653 MS.gene003370:CDS
ATCATGAGGTGAAATAAGTT+TGG 0.419407 4.1:+28028173 None:intergenic
TTCAGCCACCTGAGCCGCCT+TGG 0.429757 4.1:+28027645 None:intergenic
TGAAATTGGTTCAAAATTGA+GGG 0.433449 4.1:-28027854 MS.gene003370:CDS
AAGAAAGCATGGATTATTCC+CGG 0.443908 4.1:-28028342 MS.gene003370:CDS
CGGTCAATCCTGCACCGTCT+TGG 0.457748 4.1:-28028322 MS.gene003370:CDS
AACGAGTTCATTAACGTGGT+TGG 0.460140 4.1:+28028143 None:intergenic
CACCGTCTTGGACCTTTCAC+CGG 0.467604 4.1:-28028310 MS.gene003370:CDS
CAAGACGGTGCAGGATTGAC+CGG 0.470050 4.1:+28028323 None:intergenic
GGCTGAAGTTGAGGCGAAGA+AGG 0.470534 4.1:-28027629 MS.gene003370:CDS
CACGTTAATGAACTCGTTCA+AGG 0.472122 4.1:-28028138 MS.gene003370:CDS
GCGGAAGGAATTGCGACACT+TGG 0.472658 4.1:-28026685 MS.gene003370:CDS
TTGGACCAAGGCGGCTCAGG+TGG 0.476684 4.1:-28027650 MS.gene003370:CDS
GAAACAACTACACAGCTGTA+AGG 0.490449 4.1:-28026618 MS.gene003370:CDS
AAGACTGTCCATGAGCAAAC+AGG 0.492575 4.1:-28026406 MS.gene003370:CDS
ATTCTGATGCTTTCGCGACC+CGG 0.494061 4.1:+28028409 None:intergenic
GAGGGCAGGAGAGGGTGACA+AGG 0.500083 4.1:-28027770 MS.gene003370:CDS
GGACCAATTGTGAAGACAGC+GGG 0.501529 4.1:+28028231 None:intergenic
CAGGCATGTTGCCTCCTGCT+TGG 0.503335 4.1:-28026387 MS.gene003370:CDS
TGCTTCCATGACGATGGAGG+AGG 0.505459 4.1:-28028085 MS.gene003370:CDS
AGCAATTCTGTATGAGAAGA+AGG 0.509643 4.1:-28026800 MS.gene003370:CDS
TAAGTTTGGCATATTTGAGA+AGG 0.518370 4.1:+28028187 None:intergenic
ATGTTGCCTCCTGCTTGGAT+GGG 0.519619 4.1:-28026382 MS.gene003370:CDS
AAGACAGCGGGGAGAACAAA+AGG 0.520506 4.1:+28028243 None:intergenic
GCAACATGCCTGTTTGCTCA+TGG 0.521020 4.1:+28026398 None:intergenic
AAGCAATTGGTGGATGTGCC+TGG 0.526695 4.1:-28027967 MS.gene003370:CDS
GAGAACAAAAGGAAGTTTCT+CGG 0.527868 4.1:+28028254 None:intergenic
GGCAGGAGAGGGTGACAAGG+AGG 0.532326 4.1:-28027767 MS.gene003370:CDS
ATTAGTATTTGGACTAATGG+TGG 0.532835 4.1:-28026514 MS.gene003370:CDS
TGAAAGGTCCAAGACGGTGC+AGG 0.535402 4.1:+28028314 None:intergenic
AGCAACTTCTTTCATAGCCA+TGG 0.536125 4.1:+28026459 None:intergenic
CTAAGGGAAGCTGAGTTACA+AGG 0.541402 4.1:-28027598 MS.gene003370:CDS
GGCTCAAGCTAATTCTGAGT+TGG 0.541784 4.1:-28027689 MS.gene003370:CDS
GGCTCAGGTGGCTGAAGTTG+AGG 0.543832 4.1:-28027638 MS.gene003370:CDS
GAAGAAGGCTGTGGCTCTAA+GGG 0.545005 4.1:-28027614 MS.gene003370:CDS
ACTAATGGTGGTGATAATAG+TGG 0.546356 4.1:-28026502 MS.gene003370:CDS
GTTCATTAACGTGGTTGGAG+TGG 0.546435 4.1:+28028148 None:intergenic
TGGAAGCCAAGAATCAAGCT+AGG 0.548842 4.1:-28027906 MS.gene003370:CDS
CGCTGCTTCCATGACGATGG+AGG 0.549128 4.1:-28028088 MS.gene003370:CDS
GATAAGGATCCCATCCAAGC+AGG 0.550974 4.1:+28026373 None:intergenic
TGCGATGCAGAGGGCAGGAG+AGG 0.552588 4.1:-28027779 MS.gene003370:CDS
GTTATTGCGATGCAGAGGGC+AGG 0.553673 4.1:-28027784 MS.gene003370:CDS
AGTTGAGGCGAAGAAGGCTG+TGG 0.558891 4.1:-28027623 MS.gene003370:CDS
CGGAAGGAATTGCGACACTT+GGG 0.562902 4.1:-28026684 MS.gene003370:CDS
GCAGGAGAGGGTGACAAGGA+GGG 0.564117 4.1:-28027766 MS.gene003370:CDS
TGCAAATGTTAAGCAATTGG+TGG 0.565284 4.1:-28027977 MS.gene003370:CDS
ATTGAACACTAGCTTTGCTA+AGG 0.566032 4.1:+28027521 None:intergenic
CTCGTTCAAGGAATCATCGA+AGG 0.568467 4.1:-28028126 MS.gene003370:CDS
ATGTGTAGTTTACCGGTGAA+AGG 0.570075 4.1:+28028298 None:intergenic
AGTACACTTCTCAATGCACT+TGG 0.570595 4.1:-28026640 MS.gene003370:CDS
GAAGAAAGCGGCTTGGACCA+AGG 0.572319 4.1:-28027662 MS.gene003370:CDS
GGCAGAAGCACAGAAAGCGT+CGG 0.572484 4.1:-28026773 MS.gene003370:CDS
GAAAACCTCCTCCATCGTCA+TGG 0.573319 4.1:+28028080 None:intergenic
ATGGCTATGAAAGAAGTTGC+TGG 0.575925 4.1:-28026457 MS.gene003370:CDS
ATTGGTTCAAAATTGAGGGA+AGG 0.580771 4.1:-28027850 MS.gene003370:CDS
GCGACACTTGGGAATGCTCA+AGG 0.583461 4.1:-28026673 MS.gene003370:CDS
TGGCTAAGAAGAAAGCGGCT+TGG 0.585206 4.1:-28027669 MS.gene003370:CDS
AAACAACTACACAGCTGTAA+GGG 0.585417 4.1:-28026617 MS.gene003370:CDS
ATCAAGAATGAAAATTTACC+GGG 0.586313 4.1:-28028427 None:intergenic
CGAAGGTTATTGCGATGCAG+AGG 0.587186 4.1:-28027789 MS.gene003370:CDS
GCGATGCAGAGGGCAGGAGA+GGG 0.587192 4.1:-28027778 MS.gene003370:CDS
AAGACGGTGCAGGATTGACC+GGG 0.589066 4.1:+28028324 None:intergenic
GAATCAAAGAGAGGCTGAAG+TGG 0.589954 4.1:-28027710 MS.gene003370:CDS
CCTCGCTGCTTCCATGACGA+TGG 0.591174 4.1:-28028091 MS.gene003370:CDS
CTTGAACGAGTTCATTAACG+TGG 0.594211 4.1:+28028139 None:intergenic
TCTGTATGAGAAGAAGGCAG+AGG 0.600808 4.1:-28026794 MS.gene003370:CDS
AGTTACAAGGTGAAGTTGAG+AGG 0.609202 4.1:-28027585 MS.gene003370:CDS
TTTGGGTCAAAAGACGCAGA+TGG 0.613836 4.1:-28027926 MS.gene003370:CDS
GTGTCAGAAGCTAAGATGAA+AGG 0.614706 4.1:-28027877 MS.gene003370:CDS
GTAGGTACAAGAAGCAAACT+GGG 0.614898 4.1:-28026851 MS.gene003370:intron
ACAAGGAGGGAATTAAAGTG+AGG 0.616761 4.1:-28027753 MS.gene003370:CDS
TCATGGACAGTCTTAAACAA+AGG 0.619848 4.1:+28026415 None:intergenic
GATTGCTAAGATTAATGCTG+AGG 0.627521 4.1:-28026560 MS.gene003370:CDS
TCGTTCAAGGAATCATCGAA+GGG 0.628409 4.1:-28028125 MS.gene003370:CDS
TGAGTTGGCTAAGAAGAAAG+CGG 0.631786 4.1:-28027674 MS.gene003370:CDS
TATGAGAAATACTCATGACC+AGG 0.635081 4.1:+28027949 None:intergenic
GAAGGTTATTGCGATGCAGA+GGG 0.636072 4.1:-28027788 MS.gene003370:CDS
GGAAGCCAAGAATCAAGCTA+GGG 0.640292 4.1:-28027905 MS.gene003370:CDS
TGATAAAACTGAAGGTGCCA+TGG 0.647694 4.1:-28026476 MS.gene003370:CDS
TGGACAGTCTTAAACAAAGG+TGG 0.649112 4.1:+28026418 None:intergenic
TAGTGTTCAATATGAAACCA+AGG 0.651992 4.1:-28027509 MS.gene003370:intron
GAAAATTGATGCAGAGACGA+AGG 0.652587 4.1:-28027806 MS.gene003370:CDS
GATTACTTGATGATAAATGG+TGG 0.658453 4.1:-28026595 MS.gene003370:CDS
AATGGTGGTGATAATAGTGG+TGG 0.661841 4.1:-28026499 MS.gene003370:CDS
AAGGATCCCATCCAAGCAGG+AGG 0.669522 4.1:+28026376 None:intergenic
TGAAAATACCTGTTATCACA+AGG 0.669974 4.1:+28028385 None:intergenic
TACCGGTGAAAGGTCCAAGA+CGG 0.673165 4.1:+28028308 None:intergenic
TGGTTGGAGTGGCGATCATG+AGG 0.675682 4.1:+28028159 None:intergenic
AGTTTATGAGAATCAAAGAG+AGG 0.679157 4.1:-28027719 MS.gene003370:CDS
AGTGGTGGTGATAAAACTGA+AGG 0.688224 4.1:-28026484 MS.gene003370:CDS
CTTTCCTGATCGTCAACACG+AGG 0.689163 4.1:+28028210 None:intergenic
CGTTCAAGGAATCATCGAAG+GGG 0.697360 4.1:-28028124 MS.gene003370:CDS
GACCAATTGTGAAGACAGCG+GGG 0.714278 4.1:+28028232 None:intergenic
ATTAATGCTGAGGCTATACG+CGG 0.715276 4.1:-28026550 MS.gene003370:CDS
AGGACCAATTGTGAAGACAG+CGG 0.726189 4.1:+28028230 None:intergenic
CCATCGTCATGGAAGCAGCG+AGG 0.728643 4.1:+28028091 None:intergenic
GAAAGCGGCTTGGACCAAGG+CGG 0.735762 4.1:-28027659 MS.gene003370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAGATGTATAATAAAT+AGG - chr4.1:28027587-28027606 MS.gene003370:CDS 10.0%
!! AAAAAGATGTATAATAAATA+GGG - chr4.1:28027588-28027607 MS.gene003370:CDS 10.0%
!! AAAAGATGTATAATAAATAG+GGG - chr4.1:28027589-28027608 MS.gene003370:CDS 15.0%
!! AAAGAAATAATTAATGTAGT+TGG - chr4.1:28027621-28027640 MS.gene003370:CDS 15.0%
!! AAGTTATATATATATTTGCT+TGG + chr4.1:28027304-28027323 None:intergenic 15.0%
!! TTAAATATTTACTTAAGTGT+TGG - chr4.1:28027853-28027872 MS.gene003370:CDS 15.0%
!!! TTTTTTGGTGATTAAAATTT+TGG - chr4.1:28027898-28027917 MS.gene003370:CDS 15.0%
!! AGTGAATTATAATTTCATGA+TGG - chr4.1:28027357-28027376 MS.gene003370:intron 20.0%
!! GACAAAAAAAATTCTCAAAA+GGG - chr4.1:28027671-28027690 MS.gene003370:CDS 20.0%
!! GTAATTTGAATTGAAGAATA+TGG - chr4.1:28027754-28027773 MS.gene003370:CDS 20.0%
!! GTAGTATAAAATTAATCATG+TGG - chr4.1:28027714-28027733 MS.gene003370:CDS 20.0%
!!! ATAACTATGGATTACTTTTT+TGG - chr4.1:28027883-28027902 MS.gene003370:CDS 20.0%
! AAGATTAGTATTTGGACTAA+TGG - chr4.1:28028260-28028279 MS.gene003370:CDS 25.0%
! AATCAGCAATAAATAGCATA+TGG + chr4.1:28027564-28027583 None:intergenic 25.0%
! AATGTAGTTGGAAATAACAA+AGG - chr4.1:28027633-28027652 MS.gene003370:CDS 25.0%
! ATGTAGTTGGAAATAACAAA+GGG - chr4.1:28027634-28027653 MS.gene003370:CDS 25.0%
! CATGAGTATTTCTCATATTT+GGG - chr4.1:28026834-28026853 MS.gene003370:CDS 25.0%
! GGACAAAAAAAATTCTCAAA+AGG - chr4.1:28027670-28027689 MS.gene003370:CDS 25.0%
! TCATGAGTATTTCTCATATT+TGG - chr4.1:28026833-28026852 MS.gene003370:CDS 25.0%
! TTAGTCCAAATACTAATCTT+AGG + chr4.1:28028260-28028279 None:intergenic 25.0%
!! ATTAAAATTTTGGATGGTGT+AGG - chr4.1:28027908-28027927 MS.gene003370:CDS 25.0%
!! TCAAAAGGGTCTTATAATTA+GGG - chr4.1:28027685-28027704 MS.gene003370:CDS 25.0%
!! TGAAATTGGTTCAAAATTGA+GGG - chr4.1:28026923-28026942 MS.gene003370:intron 25.0%
!! TGTTGGCTATTATATAACTA+TGG - chr4.1:28027870-28027889 MS.gene003370:CDS 25.0%
!!! TTGGTGATTAAAATTTTGGA+TGG - chr4.1:28027902-28027921 MS.gene003370:CDS 25.0%
AAACAAGCTGAAAAGTGAAA+AGG + chr4.1:28027795-28027814 None:intergenic 30.0%
AGTTTATGAGAATCAAAGAG+AGG - chr4.1:28027058-28027077 MS.gene003370:intron 30.0%
ATCATGAGGTGAAATAAGTT+TGG + chr4.1:28026607-28026626 None:intergenic 30.0%
ATTAGTATTTGGACTAATGG+TGG - chr4.1:28028263-28028282 MS.gene003370:CDS 30.0%
CAATGCAAATGTTAAGCAAT+TGG - chr4.1:28026797-28026816 MS.gene003370:CDS 30.0%
TAAACTAACTCTCTCTCTTA+AGG + chr4.1:28027535-28027554 None:intergenic 30.0%
TAAGTTTGGCATATTTGAGA+AGG + chr4.1:28026593-28026612 None:intergenic 30.0%
TAGTGTTCAATATGAAACCA+AGG - chr4.1:28027268-28027287 MS.gene003370:intron 30.0%
TCAAACTCAAAAAGAAAGCA+TGG - chr4.1:28026424-28026443 MS.gene003370:CDS 30.0%
TGAAAATACCTGTTATCACA+AGG + chr4.1:28026395-28026414 None:intergenic 30.0%
TGTAGTTGGAAATAACAAAG+GGG - chr4.1:28027635-28027654 MS.gene003370:CDS 30.0%
TTGAGCCTAAGATTAGTATT+TGG - chr4.1:28028252-28028271 MS.gene003370:CDS 30.0%
! AACAAAGGGGTCTTATAAAA+AGG - chr4.1:28027648-28027667 MS.gene003370:CDS 30.0%
! ACAAAGGGGTCTTATAAAAA+GGG - chr4.1:28027649-28027668 MS.gene003370:CDS 30.0%
! AGGGATTACTTGATGATAAA+TGG - chr4.1:28028179-28028198 MS.gene003370:CDS 30.0%
! ATCATGTGGTAATTAACTTG+TGG - chr4.1:28027728-28027747 MS.gene003370:CDS 30.0%
! CTCAAAAGGGTCTTATAATT+AGG - chr4.1:28027684-28027703 MS.gene003370:CDS 30.0%
! GATTACTTGATGATAAATGG+TGG - chr4.1:28028182-28028201 MS.gene003370:CDS 30.0%
! GTGAAATTGGTTCAAAATTG+AGG - chr4.1:28026922-28026941 MS.gene003370:intron 30.0%
! TTAAGAGCTTTTGTCAGATA+AGG + chr4.1:28028423-28028442 None:intergenic 30.0%
AAACAACTACACAGCTGTAA+GGG - chr4.1:28028160-28028179 MS.gene003370:CDS 35.0%
AATCACGGGAATTAAGAAAG+TGG - chr4.1:28027460-28027479 MS.gene003370:intron 35.0%
ACTTCGAATGTGTAGTTTAC+CGG + chr4.1:28026489-28026508 None:intergenic 35.0%
AGAGCTATACGCAAAGAAAA+AGG - chr4.1:28028067-28028086 MS.gene003370:CDS 35.0%
AGCAAAAGGGTCCTATAATT+AGG + chr4.1:28027438-28027457 None:intergenic 35.0%
AGCAATTCTGTATGAGAAGA+AGG - chr4.1:28027977-28027996 MS.gene003370:CDS 35.0%
GAAACAGTTGTGATCTATGT+TGG - chr4.1:28027820-28027839 MS.gene003370:CDS 35.0%
GAGAACAAAAGGAAGTTTCT+CGG + chr4.1:28026526-28026545 None:intergenic 35.0%
GATTGCTAAGATTAATGCTG+AGG - chr4.1:28028217-28028236 MS.gene003370:CDS 35.0%
GCAAAAGGGTCCTATAATTA+GGG + chr4.1:28027437-28027456 None:intergenic 35.0%
GCTAAGATGAAAGGTGAAAT+TGG - chr4.1:28026909-28026928 MS.gene003370:intron 35.0%
GCTGTACAAGAAACAAAAAG+AGG - chr4.1:28027950-28027969 MS.gene003370:CDS 35.0%
TATGAGAAATACTCATGACC+AGG + chr4.1:28026831-28026850 None:intergenic 35.0%
TCAGAATACCTTGTGATAAC+AGG - chr4.1:28026384-28026403 MS.gene003370:CDS 35.0%
TCATGGACAGTCTTAAACAA+AGG + chr4.1:28028365-28028384 None:intergenic 35.0%
TGCAAATGTTAAGCAATTGG+TGG - chr4.1:28026800-28026819 MS.gene003370:CDS 35.0%
! AAGAAAGCATGGATTATTCC+CGG - chr4.1:28026435-28026454 MS.gene003370:CDS 35.0%
! AAGGGTCTTATAATTAGGGA+CGG - chr4.1:28027689-28027708 MS.gene003370:CDS 35.0%
! ACTAATGGTGGTGATAATAG+TGG - chr4.1:28028275-28028294 MS.gene003370:CDS 35.0%
! ATTGGTTCAAAATTGAGGGA+AGG - chr4.1:28026927-28026946 MS.gene003370:intron 35.0%
!! ATTCCCGTGATTTTAGCAAA+AGG + chr4.1:28027452-28027471 None:intergenic 35.0%
!! ATTGAACACTAGCTTTGCTA+AGG + chr4.1:28027259-28027278 None:intergenic 35.0%
!! CAGCTTGAGTTGAATCAATT+CGG - chr4.1:28026762-28026781 MS.gene003370:CDS 35.0%
!! TTCCCGTGATTTTAGCAAAA+GGG + chr4.1:28027451-28027470 None:intergenic 35.0%
AACGAGTTCATTAACGTGGT+TGG + chr4.1:28026637-28026656 None:intergenic 40.0%
AAGCTGAAAAGTGAAAAGGC+TGG + chr4.1:28027791-28027810 None:intergenic 40.0%
AAGGGTCCTATAATTAGGGA+CGG + chr4.1:28027433-28027452 None:intergenic 40.0%
ACAAGGAGGGAATTAAAGTG+AGG - chr4.1:28027024-28027043 MS.gene003370:intron 40.0%
AGCAACTTCTTTCATAGCCA+TGG + chr4.1:28028321-28028340 None:intergenic 40.0%
AGTACACTTCTCAATGCACT+TGG - chr4.1:28028137-28028156 MS.gene003370:CDS 40.0%
AGTTACAAGGTGAAGTTGAG+AGG - chr4.1:28027192-28027211 MS.gene003370:intron 40.0%
ATACGCAAAGAAAAAGGAGG+CGG - chr4.1:28028073-28028092 MS.gene003370:CDS 40.0%
ATGGCTATGAAAGAAGTTGC+TGG - chr4.1:28028320-28028339 MS.gene003370:CDS 40.0%
ATGTGTAGTTTACCGGTGAA+AGG + chr4.1:28026482-28026501 None:intergenic 40.0%
ATTAATGCTGAGGCTATACG+CGG - chr4.1:28028227-28028246 MS.gene003370:CDS 40.0%
CACGTTAATGAACTCGTTCA+AGG - chr4.1:28026639-28026658 MS.gene003370:CDS 40.0%
CTTGAACGAGTTCATTAACG+TGG + chr4.1:28026641-28026660 None:intergenic 40.0%
GAAAATTGATGCAGAGACGA+AGG - chr4.1:28026971-28026990 MS.gene003370:intron 40.0%
GAAACAACTACACAGCTGTA+AGG - chr4.1:28028159-28028178 MS.gene003370:CDS 40.0%
GCTATACGCAAAGAAAAAGG+AGG - chr4.1:28028070-28028089 MS.gene003370:CDS 40.0%
GTAGGTACAAGAAGCAAACT+GGG - chr4.1:28027926-28027945 MS.gene003370:CDS 40.0%
GTGTCAGAAGCTAAGATGAA+AGG - chr4.1:28026900-28026919 MS.gene003370:intron 40.0%
TCGTTCAAGGAATCATCGAA+GGG - chr4.1:28026652-28026671 MS.gene003370:CDS 40.0%
TGCTTGGACTAAACTAACCT+TGG + chr4.1:28027288-28027307 None:intergenic 40.0%
TGGACAGTCTTAAACAAAGG+TGG + chr4.1:28028362-28028381 None:intergenic 40.0%
TGTAGGTACAAGAAGCAAAC+TGG - chr4.1:28027925-28027944 MS.gene003370:CDS 40.0%
! AATGGTGGTGATAATAGTGG+TGG - chr4.1:28028278-28028297 MS.gene003370:CDS 40.0%
! AGTGGTGGTGATAAAACTGA+AGG - chr4.1:28028293-28028312 MS.gene003370:CDS 40.0%
! GACCCTTTTGCTAAAATCAC+GGG - chr4.1:28027446-28027465 MS.gene003370:intron 40.0%
! GGACCCTTTTGCTAAAATCA+CGG - chr4.1:28027445-28027464 MS.gene003370:intron 40.0%
! TGAGTTGGCTAAGAAGAAAG+CGG - chr4.1:28027103-28027122 MS.gene003370:intron 40.0%
!! TGATAAAACTGAAGGTGCCA+TGG - chr4.1:28028301-28028320 MS.gene003370:CDS 40.0%
AAGACTGTCCATGAGCAAAC+AGG - chr4.1:28028371-28028390 MS.gene003370:CDS 45.0%
AGGACCAATTGTGAAGACAG+CGG + chr4.1:28026550-28026569 None:intergenic 45.0%
CGTTCAAGGAATCATCGAAG+GGG - chr4.1:28026653-28026672 MS.gene003370:CDS 45.0%
CTAAGGGAAGCTGAGTTACA+AGG - chr4.1:28027179-28027198 MS.gene003370:intron 45.0%
CTACCTCCGTCCCTAATTAT+AGG - chr4.1:28027424-28027443 MS.gene003370:intron 45.0%
CTCGTTCAAGGAATCATCGA+AGG - chr4.1:28026651-28026670 MS.gene003370:CDS 45.0%
GAAGGTTATTGCGATGCAGA+GGG - chr4.1:28026989-28027008 MS.gene003370:intron 45.0%
GAATCAAAGAGAGGCTGAAG+TGG - chr4.1:28027067-28027086 MS.gene003370:intron 45.0%
GGAAGCCAAGAATCAAGCTA+GGG - chr4.1:28026872-28026891 MS.gene003370:intron 45.0%
GGCTCAAGCTAATTCTGAGT+TGG - chr4.1:28027088-28027107 MS.gene003370:intron 45.0%
GTCAACCCTAGCTTGATTCT+TGG + chr4.1:28026880-28026899 None:intergenic 45.0%
GTTCATTAACGTGGTTGGAG+TGG + chr4.1:28026632-28026651 None:intergenic 45.0%
TCTGTATGAGAAGAAGGCAG+AGG - chr4.1:28027983-28028002 MS.gene003370:CDS 45.0%
TGGAAGCCAAGAATCAAGCT+AGG - chr4.1:28026871-28026890 MS.gene003370:intron 45.0%
TTTGGGTCAAAAGACGCAGA+TGG - chr4.1:28026851-28026870 MS.gene003370:intron 45.0%
! GGTCTTATAATTAGGGACGG+AGG - chr4.1:28027692-28027711 MS.gene003370:CDS 45.0%
AAGACAGCGGGGAGAACAAA+AGG + chr4.1:28026537-28026556 None:intergenic 50.0%
ATGTTGCCTCCTGCTTGGAT+GGG - chr4.1:28028395-28028414 MS.gene003370:CDS 50.0%
ATTCTGATGCTTTCGCGACC+CGG + chr4.1:28026371-28026390 None:intergenic 50.0%
CGAAGGTTATTGCGATGCAG+AGG - chr4.1:28026988-28027007 MS.gene003370:intron 50.0%
CGGAAGGAATTGCGACACTT+GGG - chr4.1:28028093-28028112 MS.gene003370:CDS 50.0%
CTTTCCTGATCGTCAACACG+AGG + chr4.1:28026570-28026589 None:intergenic 50.0%
GAAAACCTCCTCCATCGTCA+TGG + chr4.1:28026700-28026719 None:intergenic 50.0%
GACCAATTGTGAAGACAGCG+GGG + chr4.1:28026548-28026567 None:intergenic 50.0%
GATAAGGATCCCATCCAAGC+AGG + chr4.1:28028407-28028426 None:intergenic 50.0%
GCAAAGAAAAAGGAGGCGGA+AGG - chr4.1:28028077-28028096 MS.gene003370:CDS 50.0%
GCAACATGCCTGTTTGCTCA+TGG + chr4.1:28028382-28028401 None:intergenic 50.0%
GGACCAATTGTGAAGACAGC+GGG + chr4.1:28026549-28026568 None:intergenic 50.0%
GGTCCTATAATTAGGGACGG+AGG + chr4.1:28027430-28027449 None:intergenic 50.0%
TACCGGTGAAAGGTCCAAGA+CGG + chr4.1:28026472-28026491 None:intergenic 50.0%
TGGCTAAGAAGAAAGCGGCT+TGG - chr4.1:28027108-28027127 MS.gene003370:intron 50.0%
! AAGCAATTGGTGGATGTGCC+TGG - chr4.1:28026810-28026829 MS.gene003370:CDS 50.0%
! ACGATGGAGGAGGTTTTCAG+AGG - chr4.1:28026702-28026721 MS.gene003370:CDS 50.0%
!! GAAGAAGGCTGTGGCTCTAA+GGG - chr4.1:28027163-28027182 MS.gene003370:intron 50.0%
AAGACGGTGCAGGATTGACC+GGG + chr4.1:28026456-28026475 None:intergenic 55.0%
AAGGATCCCATCCAAGCAGG+AGG + chr4.1:28028404-28028423 None:intergenic 55.0%
AGTTGAGGCGAAGAAGGCTG+TGG - chr4.1:28027154-28027173 MS.gene003370:intron 55.0%
CAAGACGGTGCAGGATTGAC+CGG + chr4.1:28026457-28026476 None:intergenic 55.0%
CACCGTCTTGGACCTTTCAC+CGG - chr4.1:28026467-28026486 MS.gene003370:CDS 55.0%
CATGTTGCCTCCTGCTTGGA+TGG - chr4.1:28028394-28028413 MS.gene003370:CDS 55.0%
CTCCCCGCTGTCTTCACAAT+TGG - chr4.1:28026543-28026562 MS.gene003370:CDS 55.0%
GCGACACTTGGGAATGCTCA+AGG - chr4.1:28028104-28028123 MS.gene003370:CDS 55.0%
GCGGAAGGAATTGCGACACT+TGG - chr4.1:28028092-28028111 MS.gene003370:CDS 55.0%
GGCTGAAGTTGAGGCGAAGA+AGG - chr4.1:28027148-28027167 MS.gene003370:intron 55.0%
GTTATTGCGATGCAGAGGGC+AGG - chr4.1:28026993-28027012 MS.gene003370:intron 55.0%
TGAAAGGTCCAAGACGGTGC+AGG + chr4.1:28026466-28026485 None:intergenic 55.0%
TGCTTCCATGACGATGGAGG+AGG - chr4.1:28026692-28026711 MS.gene003370:CDS 55.0%
TGGTTGGAGTGGCGATCATG+AGG + chr4.1:28026621-28026640 None:intergenic 55.0%
! CGAAGAAGGCTGTGGCTCTA+AGG - chr4.1:28027162-28027181 MS.gene003370:intron 55.0%
! GAAGAAAGCGGCTTGGACCA+AGG - chr4.1:28027115-28027134 MS.gene003370:intron 55.0%
! GGCAGAAGCACAGAAAGCGT+CGG - chr4.1:28028004-28028023 MS.gene003370:CDS 55.0%
! TGGTCCTCGTGTTGACGATC+AGG - chr4.1:28026563-28026582 MS.gene003370:CDS 55.0%
CAGGCATGTTGCCTCCTGCT+TGG - chr4.1:28028390-28028409 MS.gene003370:CDS 60.0%
CCATCGTCATGGAAGCAGCG+AGG + chr4.1:28026689-28026708 None:intergenic 60.0%
CCTCGCTGCTTCCATGACGA+TGG - chr4.1:28026686-28026705 MS.gene003370:CDS 60.0%
CGCTGCTTCCATGACGATGG+AGG - chr4.1:28026689-28026708 MS.gene003370:CDS 60.0%
CGGTCAATCCTGCACCGTCT+TGG - chr4.1:28026455-28026474 MS.gene003370:CDS 60.0%
GCAGGAGAGGGTGACAAGGA+GGG - chr4.1:28027011-28027030 MS.gene003370:intron 60.0%
GGCTCAGGTGGCTGAAGTTG+AGG - chr4.1:28027139-28027158 MS.gene003370:intron 60.0%
! GAAAGCGGCTTGGACCAAGG+CGG - chr4.1:28027118-28027137 MS.gene003370:intron 60.0%
GAGGGCAGGAGAGGGTGACA+AGG - chr4.1:28027007-28027026 MS.gene003370:intron 65.0%
GCGATGCAGAGGGCAGGAGA+GGG - chr4.1:28026999-28027018 MS.gene003370:intron 65.0%
GGCAGGAGAGGGTGACAAGG+AGG - chr4.1:28027010-28027029 MS.gene003370:intron 65.0%
TGCGATGCAGAGGGCAGGAG+AGG - chr4.1:28026998-28027017 MS.gene003370:intron 65.0%
TTCAGCCACCTGAGCCGCCT+TGG + chr4.1:28027135-28027154 None:intergenic 65.0%
TTGGACCAAGGCGGCTCAGG+TGG - chr4.1:28027127-28027146 MS.gene003370:intron 65.0%
GGCTTGGACCAAGGCGGCTC+AGG - chr4.1:28027124-28027143 MS.gene003370:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 28026357 28028442 28026357 ID=MS.gene003370
chr4.1 mRNA 28026357 28028442 28026357 ID=MS.gene003370.t1;Parent=MS.gene003370
chr4.1 exon 28027510 28028442 28027510 ID=MS.gene003370.t1.exon1;Parent=MS.gene003370.t1
chr4.1 CDS 28027510 28028442 28027510 ID=cds.MS.gene003370.t1;Parent=MS.gene003370.t1
chr4.1 exon 28026357 28026869 28026357 ID=MS.gene003370.t1.exon2;Parent=MS.gene003370.t1
chr4.1 CDS 28026357 28026869 28026357 ID=cds.MS.gene003370.t1;Parent=MS.gene003370.t1
Gene Sequence

>MS.gene003370

ATGAAAATTTACCGGGTCGCGAAAGCATCAGAATACCTTGTGATAACAGGTATTTTCATCAAAGACATCAAACTCAAAAAGAAAGCATGGATTATTCCCGGTCAATCCTGCACCGTCTTGGACCTTTCACCGGTAAACTACACATTCGAAGTTCAAGCTATGAGTGCCGAGAAACTTCCTTTTGTTCTCCCCGCTGTCTTCACAATTGGTCCTCGTGTTGACGATCAGGAAAGCCTTCTCAAATATGCCAAACTTATTTCACCTCATGATCGCCACTCCAACCACGTTAATGAACTCGTTCAAGGAATCATCGAAGGGGAGACACGTGTCCTCGCTGCTTCCATGACGATGGAGGAGGTTTTCAGAGGAACAAAACAGTTTAAACAAGAAGTATTCGACAAAGTTCAGCTTGAGTTGAATCAATTCGGATTATTGATTTACAATGCAAATGTTAAGCAATTGGTGGATGTGCCTGGTCATGAGTATTTCTCATATTTGGGTCAAAAGACGCAGATGGAAGCCAAGAATCAAGCTAGGGTTGACGTGTCAGAAGCTAAGATGAAAGGTGAAATTGGTTCAAAATTGAGGGAAGGACAAACGCTGCAGAATGCAGCGAAAATTGATGCAGAGACGAAGGTTATTGCGATGCAGAGGGCAGGAGAGGGTGACAAGGAGGGAATTAAAGTGAGGAGTGAAGTGAAAGTTTATGAGAATCAAAGAGAGGCTGAAGTGGCTCAAGCTAATTCTGAGTTGGCTAAGAAGAAAGCGGCTTGGACCAAGGCGGCTCAGGTGGCTGAAGTTGAGGCGAAGAAGGCTGTGGCTCTAAGGGAAGCTGAGTTACAAGGTGAAGTTGAGAGGATGAATGCTTTGACTACTACTGAAAAGCTTAAAGCAGAGTTCCTTAGCAAAGCTAGTGTTCAATATGAAACCAAGGTTAGTTTAGTCCAAGCAAATATATATATAACTTCATTCATTTTTTTTTTGCTGAAATTAAATAGTAAGTGAATTATAATTTCATGATGGAAGAGTTGTTGAACATTTATCAGTTAACTTACTGTATCATATTACTACCTCCGTCCCTAATTATAGGACCCTTTTGCTAAAATCACGGGAATTAAGAAAGTGGATATTTGTATTAAAGTTGTTTGTAATCACTATTGTTTTTACAATTTTATCCTTAAGAGAGAGAGTTAGTTTATGTTTTCCATATGCTATTTATTGCTGATTGAAAAAAAAGATGTATAATAAATAGGGGCATGTATGTAAAGAAATAATTAATGTAGTTGGAAATAACAAAGGGGTCTTATAAAAAGGGACAAAAAAAATTCTCAAAAGGGTCTTATAATTAGGGACGGAGGTAGTATAAAATTAATCATGTGGTAATTAACTTGTGGTATGTAATTTGAATTGAAGAATATGGAAATATAACTACCAGCCTTTTCACTTTTCAGCTTGTTTAACATGAAACAGTTGTGATCTATGTTGGATTAATGAATTTAAATATTTACTTAAGTGTTGGCTATTATATAACTATGGATTACTTTTTTGGTGATTAAAATTTTGGATGGTGTAGGTACAAGAAGCAAACTGGGAGCTGTACAAGAAACAAAAAGAGGCTGAAGCAATTCTGTATGAGAAGAAGGCAGAGGCAGAAGCACAGAAAGCGTCGGCAGATGCAACATTTTATGCTCGCAAGCAAGAAGCTGAAGCAGAGCTATACGCAAAGAAAAAGGAGGCGGAAGGAATTGCGACACTTGGGAATGCTCAAGGAGCGTATGTAAGTACACTTCTCAATGCACTTGGAAACAACTACACAGCTGTAAGGGATTACTTGATGATAAATGGTGGCATGTTTCAAGAGATTGCTAAGATTAATGCTGAGGCTATACGCGGACTTGAGCCTAAGATTAGTATTTGGACTAATGGTGGTGATAATAGTGGTGGTGATAAAACTGAAGGTGCCATGGCTATGAAAGAAGTTGCTGGTGTCTACAAGATGTTGCCACCTTTGTTTAAGACTGTCCATGAGCAAACAGGCATGTTGCCTCCTGCTTGGATGGGATCCTTATCTGACAAAAGCTCTTAA
Protein sequence

>MS.gene003370.t1

MKIYRVAKASEYLVITGIFIKDIKLKKKAWIIPGQSCTVLDLSPVNYTFEVQAMSAEKLPFVLPAVFTIGPRVDDQESLLKYAKLISPHDRHSNHVNELVQGIIEGETRVLAASMTMEEVFRGTKQFKQEVFDKVQLELNQFGLLIYNANVKQLVDVPGHEYFSYLGQKTQMEAKNQARVDVSEAKMKGEIGSKLREGQTLQNAAKIDAETKVIAMQRAGEGDKEGIKVRSEVKVYENQREAEVAQANSELAKKKAAWTKAAQVAEVEAKKAVALREAELQGEVERMNALTTTEKLKAEFLSKASVQYETKVQEANWELYKKQKEAEAILYEKKAEAEAQKASADATFYARKQEAEAELYAKKKEAEGIATLGNAQGAYVSTLLNALGNNYTAVRDYLMINGGMFQEIAKINAEAIRGLEPKISIWTNGGDNSGGDKTEGAMAMKEVAGVYKMLPPLFKTVHEQTGMLPPAWMGSLSDKSS